Moc10g03530 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g03530
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	proline-rich receptor-like protein kinase PERK2
Location	chr10: 2503671 .. 2504021 (+)
RNA-Seq Expression	Moc10g03530
Synteny	Moc10g03530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTCCAAGTTCTTGATCATTGCATTATTCATGGTCGCTTTGGCACTTTCGAGCGTCGACGCCGCCCGAGCTGCCCGGCATCTTCTGCAAACCGCTCCACCCTCTCTGCCCAACTTCCCGGTGGTGCCAATGGGGCTCCCGCCGATGCCGATACCGACTTTGCCAAACCCTACTACAGGGCCGAGTATTATTCCTTCGCTTCCAAATCTGCCTCAGCCCACATTGCCTAGGGTTACACTCCCTCCGCTCCCAAGCTTCCCGGTGAACATCCCGAGCGGGGCAACGCTGCCTTCTCTACCGTCGATCCCATTTCTCGCGCCTCCGCCAGCAACTTCTTCACCCTAA

mRNA sequence

ATGGCTTCTTCCAAGTTCTTGATCATTGCATTATTCATGGTCGCTTTGGCACTTTCGAGCGTCGACGCCGCCCGAGCTGCCCGGCATCTTCTGCAAACCGCTCCACCCTCTCTGCCCAACTTCCCGGTGGTGCCAATGGGGCTCCCGCCGATGCCGATACCGACTTTGCCAAACCCTACTACAGGGCCGAGTATTATTCCTTCGCTTCCAAATCTGCCTCAGCCCACATTGCCTAGGGTTACACTCCCTCCGCTCCCAAGCTTCCCGGTGAACATCCCGAGCGGGGCAACGCTGCCTTCTCTACCGTCGATCCCATTTCTCGCGCCTCCGCCAGCAACTTCTTCACCCTAA

Coding sequence (CDS)

ATGGCTTCTTCCAAGTTCTTGATCATTGCATTATTCATGGTCGCTTTGGCACTTTCGAGCGTCGACGCCGCCCGAGCTGCCCGGCATCTTCTGCAAACCGCTCCACCCTCTCTGCCCAACTTCCCGGTGGTGCCAATGGGGCTCCCGCCGATGCCGATACCGACTTTGCCAAACCCTACTACAGGGCCGAGTATTATTCCTTCGCTTCCAAATCTGCCTCAGCCCACATTGCCTAGGGTTACACTCCCTCCGCTCCCAAGCTTCCCGGTGAACATCCCGAGCGGGGCAACGCTGCCTTCTCTACCGTCGATCCCATTTCTCGCGCCTCCGCCAGCAACTTCTTCACCCTAA

Protein sequence

MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPTTGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP

Homology

[Close]

BLAST of Moc10g03530 vs. NCBI nr
Match: XP_022149166.1 (proline-rich receptor-like protein kinase PERK2 [Momordica charantia])

HSP 1 Score: 209.5 bits (532), Expect = 1.5e-50
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT
Sbjct: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP 117
           TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP
Sbjct: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP 116

[Close]

BLAST of Moc10g03530 vs. NCBI nr
Match: KAA0056641.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK07567.1 proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 124.8 bits (312), Expect = 4.9e-25
Identity = 82/133 (61.65%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIA FM  LALSS+D  +AARHLLQTAPP +PN PV+P G+P  P+P L NPT
Sbjct: 1   MASSKFLIIA-FMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIP--PLPALSNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLP------------------SLP 116
                   LPNLPQPTLP+V LPPLPS PVNIPSG  LP                  S P
Sbjct: 61  --------LPNLPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFP 120

[Close]

BLAST of Moc10g03530 vs. NCBI nr
Match: KAG6577257.1 (hypothetical protein SDJN03_24831, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015347.1 hypothetical protein SDJN02_22982, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 121.3 bits (303), Expect = 5.4e-24
Identity = 77/128 (60.16%), Postives = 85/128 (66.41%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIA FM  LALSS+D  +AARHLLQT PP LPN P +P G+PP          
Sbjct: 1   MASSKFLIIA-FMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPP---------- 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLP-------------SLPSIPFL 116
                +P+LPNLPQPTLP+V LPP PS PVNIPSGA LP             S PSIPFL
Sbjct: 61  -----LPTLPNLPQPTLPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFL 112

[Close]

BLAST of Moc10g03530 vs. NCBI nr
Match: XP_038904303.1 (protein PELPK1-like [Benincasa hispida])

HSP 1 Score: 119.0 bits (297), Expect = 2.7e-23
Identity = 81/126 (64.29%), Postives = 90/126 (71.43%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           M SSKFLIIA FM  LALSS+D  +AARHLLQTAPP +PN PV+P G+P  P+PTLPNPT
Sbjct: 1   MGSSKFLIIA-FMATLALSSIDLGQAARHLLQTAPPLVPNLPVIPTGIP--PLPTLPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPS------FPV----NIPSGATLP-SLPSIPFLAP 116
           T       LPNLPQPTLP+V LPPLPS       P     N PS  T+P S PSIPFL+P
Sbjct: 61  T-------LPNLPQPTLPKVNLPPLPSGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 116

[Close]

BLAST of Moc10g03530 vs. NCBI nr
Match: XP_023518061.1 (protein PELPK1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 114.4 bits (285), Expect = 6.6e-22
Identity = 77/131 (58.78%), Postives = 91/131 (69.47%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSK+LIIA FMV L  S++D  RAARHLLQTAPP++P  P +P   P  P+PT+PNPT
Sbjct: 1   MASSKYLIIA-FMVTLMFSAIDTGRAARHLLQTAPPNVPRLPSLP---PLPPLPTMPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRV--------TLPPLPSFPVNIPSG--------ATLPSLPSI 116
           TGP  IPS+P+LPQPTLP +         LPPLPSFP NIP+G          LP+LPSI
Sbjct: 61  TGPG-IPSIPSLPQPTLPTLPTTQPSLPKLPPLPSFPRNIPTGMALPPLPTTLLPNLPSI 120

[Close]

BLAST of Moc10g03530 vs. ExPASy TrEMBL
Match: A0A6J1D7J7 (proline-rich receptor-like protein kinase PERK2 OS=Momordica charantia OX=3673 GN=LOC111017657 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.3e-51
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT
Sbjct: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP 117
           TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP
Sbjct: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLPSLPSIPFLAPPPATSSP 116

[Close]

BLAST of Moc10g03530 vs. ExPASy TrEMBL
Match: A0A5D3C6P2 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00400 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.4e-25
Identity = 82/133 (61.65%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIA FM  LALSS+D  +AARHLLQTAPP +PN PV+P G+P  P+P L NPT
Sbjct: 1   MASSKFLIIA-FMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIP--PLPALSNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPSFPVNIPSGATLP------------------SLP 116
                   LPNLPQPTLP+V LPPLPS PVNIPSG  LP                  S P
Sbjct: 61  --------LPNLPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFP 120

[Close]

BLAST of Moc10g03530 vs. ExPASy TrEMBL
Match: A0A5A7ULD9 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold288G001670 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.3e-20
Identity = 75/126 (59.52%), Postives = 86/126 (68.25%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIA FM  LALSS++  +AARHLLQT PP +PN P +P G+P  P P LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIP--PFPALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPS------FPV----NIPSGATLP-SLPSIPFLAP 116
                   LPNLPQPT+P+V LPPLP+       P     N PS  T+P S PSIPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of Moc10g03530 vs. ExPASy TrEMBL
Match: A0A5D3C8R8 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00410 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.8e-20
Identity = 75/126 (59.52%), Postives = 86/126 (68.25%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIA FM  LALSS++  +AARHLLQT PP +PN P +P G+P  P P LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIP--PFPALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPS------FPV----NIPSGATLP-SLPSIPFLAP 116
                   LPNLPQPT+P+V LPPLP+       P     N PS  T+P S PSIPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of Moc10g03530 vs. ExPASy TrEMBL
Match: A0A1S3CJJ1 (proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC103501166 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.8e-20
Identity = 75/126 (59.52%), Postives = 86/126 (68.25%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAARHLLQTAPPSLPNFPVVPMGLPPMPIPTLPNPT 60
           MASSKFLIIA FM  LALSS++  +AARHLLQT PP +PN P +P G+P  P P LPNPT
Sbjct: 1   MASSKFLIIA-FMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIP--PFPALPNPT 60

Query: 61  TGPSIIPSLPNLPQPTLPRVTLPPLPS------FPV----NIPSGATLP-SLPSIPFLAP 116
                   LPNLPQPT+P+V LPPLP+       P     N PS  T+P S PSIPFL+P
Sbjct: 61  --------LPNLPQPTIPKVNLPPLPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSP 115

[Close]

BLAST of Moc10g03530 vs. TAIR 10
Match: AT4G38080.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 42.7 bits (99), Expect = 2.3e-04
Identity = 48/135 (35.56%), Postives = 62/135 (45.93%), Query Frame = 0


Query: 1   MASSKFLIIALFMVALALSSVDAARAA-RHLLQTAP-----------------PSLPNFP 60
           MA  K   +   ++AL  +S   + +A RHLLQ+ P                 P +P+ P
Sbjct: 1   MAPLKNSFVTSLVIALTFTSFFTSLSAHRHLLQSTPVTQPPALTFPPLPKTTMPPVPSLP 60

Query: 61  VVPMGLPPMPIPTLPNPTTGPSI----IPSLPNLPQPTLPRVTLPPLPS-FPVNIPSGAT 113
                  P P PTLP PT  P +    IPSLPN  QPT+P +     PS FP N P    
Sbjct: 61  TPGQQTLPQPQPTLPQPTGLPPMPSTQIPSLPNQVQPTIPNIPQINFPSNFPFNFPF--- 120

[Close]

BLAST of Moc10g03530 vs. TAIR 10
Match: AT5G15780.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 42.7 bits (99), Expect = 2.3e-04
Identity = 41/95 (43.16%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 34  APPSLPNFPVVPM--GLPPMP-IPTLPNPTTGPSIIPSLPNLPQ-PTLPRVTL------- 93
           +PPSLP  P++P    LP +P +PT P PT  P  IP++P LP  P LP V +       
Sbjct: 294 SPPSLPPIPLIPTPPTLPTIPLLPTPPTPTLPP--IPTIPTLPPLPVLPPVPIVNPPSLP 353

Query: 94  PPLPSFPVNIPSGATLPSLPSIPFL-APPPATSSP 117
           PP PSFPV +P    LP +P +P +   PPA   P
Sbjct: 354 PPPPSFPVPLPPVPGLPGIPPVPLIPGIPPAPLIP 386

The following BLAST results are available for this feature:

BLAST of Moc10g03530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149166.1	1.5e-50	100.00	proline-rich receptor-like protein kinase PERK2 [Momordica charantia]	[more]
KAA0056641.1	4.9e-25	61.65	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK0...	[more]
KAG6577257.1	5.4e-24	60.16	hypothetical protein SDJN03_24831, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038904303.1	2.7e-23	64.29	protein PELPK1-like [Benincasa hispida]	[more]
XP_023518061.1	6.6e-22	58.78	protein PELPK1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc10g03530 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc10g03530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7J7	7.3e-51	100.00	proline-rich receptor-like protein kinase PERK2 OS=Momordica charantia OX=3673 G...	[more]
A0A5D3C6P2	2.4e-25	61.65	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5A7ULD9	1.3e-20	59.52	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3C8R8	1.8e-20	59.52	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3CJJ1	1.8e-20	59.52	proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Moc10g03530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38080.1	2.3e-04	35.56	hydroxyproline-rich glycoprotein family protein	[more]
AT5G15780.1	2.3e-04	43.16	Pollen Ole e 1 allergen and extensin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044659	Protein PELPK-like	PANTHER	PTHR33088	MUCIN-2	coord: 1..116
None	No IPR available	PANTHER	PTHR33088:SF33	PROTEIN APP1-LIKE	coord: 1..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g03530.1	Moc10g03530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity