Moc10g03480 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g03480
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Glycosyltransferase AER61, uncharacterized
Location	chr10: 2472741 .. 2473332 (-)
RNA-Seq Expression	Moc10g03480
Synteny	Moc10g03480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAGACAGACATATTGGTATCCCCACATGGAGCACAGTTGACAAACCTGTTTCTAATGGACAGAAACAGCAGTGTTATGGAGTTTTTCCCCAAAGGATGGCTCAAGCTTGCAGGCATTGGCCAGTTCGTGTTCCGTTGGCTCGCAAGCTGGTCTGGAATGACGCATCAAGGTGATTGGAGAGACCCTCATGGCCCCGCCTGTCCCTATCCCGAACACGACCGTCGTTGCATGTCCGTTTATAAAAGTGGCACCATCGGGTACGCTTCTATTTTAGAATCCCATTTTCATCTCTAAACTTTTACCCGTTACCTGAGCTTCGAATGAAAGATTATTATATGCATTTATATTGAAATGTGAGTATACGGTCTAGTTTGTCTATAAATTTGAATATATTTACGATTATAATCAAAATAGAGAATAAAATAGAAAATTTTTCGTATATTTATTTTTGTTGTTGATGTTAAACAGATACAATAGAACACAGTTTTCTGAGTGGGCTAAGAATGTTCTGAATGAGGTGAAGATGAGAAAGATGGAAGAAGCAGCGGGCTCTGCAAATCATGTTCATGAATGTTTTTGTAACTAA

mRNA sequence

ATGGGGAAGACAGACATATTGGTATCCCCACATGGAGCACAGTTGACAAACCTGTTTCTAATGGACAGAAACAGCAGTGTTATGGAGTTTTTCCCCAAAGGATGGCTCAAGCTTGCAGGCATTGGCCAGTTCGTGTTCCGTTGGCTCGCAAGCTGGTCTGGAATGACGCATCAAGGTGATTGGAGAGACCCTCATGGCCCCGCCTGTCCCTATCCCGAACACGACCGTCGTTGCATGTCCGTTTATAAAAGTGGCACCATCGGATACAATAGAACACAGTTTTCTGAGTGGGCTAAGAATGTTCTGAATGAGGTGAAGATGAGAAAGATGGAAGAAGCAGCGGGCTCTGCAAATCATGTTCATGAATGTTTTTGTAACTAA

Coding sequence (CDS)

ATGGGGAAGACAGACATATTGGTATCCCCACATGGAGCACAGTTGACAAACCTGTTTCTAATGGACAGAAACAGCAGTGTTATGGAGTTTTTCCCCAAAGGATGGCTCAAGCTTGCAGGCATTGGCCAGTTCGTGTTCCGTTGGCTCGCAAGCTGGTCTGGAATGACGCATCAAGGTGATTGGAGAGACCCTCATGGCCCCGCCTGTCCCTATCCCGAACACGACCGTCGTTGCATGTCCGTTTATAAAAGTGGCACCATCGGATACAATAGAACACAGTTTTCTGAGTGGGCTAAGAATGTTCTGAATGAGGTGAAGATGAGAAAGATGGAAGAAGCAGCGGGCTCTGCAAATCATGTTCATGAATGTTTTTGTAACTAA

Protein sequence

MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGDWRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHVHECFCN

Homology

[Close]

BLAST of Moc10g03480 vs. NCBI nr
Match: XP_022141026.1 (uncharacterized protein LOC111011532 [Momordica charantia])

HSP 1 Score: 278.9 bits (712), Expect = 2.2e-71
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD
Sbjct: 316 MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 375

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHV 120
           WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHV
Sbjct: 376 WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHV 435

Query: 121 HECFCN 127
           HECFCN
Sbjct: 436 HECFCN 441

[Close]

BLAST of Moc10g03480 vs. NCBI nr
Match: XP_022984427.1 (uncharacterized protein LOC111482727 [Cucurbita maxima])

HSP 1 Score: 236.5 bits (602), Expect = 1.3e-58
Identity = 107/127 (84.25%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTN+FLMDRNSSVMEFFPKGWLKLAGIGQFV++W+ASWSGM HQG 
Sbjct: 333 MGKTDILVSPHGAQLTNMFLMDRNSSVMEFFPKGWLKLAGIGQFVYQWMASWSGMRHQGA 392

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAA-GSANH 120
           WRDPHG  CPY E DRRCMS++K GTIGYNRT FSEWAKNVLNEVK+RKM EAA  +ANH
Sbjct: 393 WRDPHGLTCPYNEDDRRCMSIFKGGTIGYNRTYFSEWAKNVLNEVKIRKMNEAAHATANH 452

Query: 121 VHECFCN 127
           VHEC CN
Sbjct: 453 VHECSCN 459

[Close]

BLAST of Moc10g03480 vs. NCBI nr
Match: XP_023541716.1 (uncharacterized protein LOC111801789 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235.3 bits (599), Expect = 2.8e-58
Identity = 106/127 (83.46%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTN+FLMDRNSSVMEFFPKGWLKLAGIGQFV++W+ASWSGM HQG 
Sbjct: 332 MGKTDILVSPHGAQLTNMFLMDRNSSVMEFFPKGWLKLAGIGQFVYQWMASWSGMRHQGA 391

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAA-GSANH 120
           WRDPHG  CPY E DRRCMS++K GTIGYNRT FSEWAKNVL+EVKMRKM+EAA  + NH
Sbjct: 392 WRDPHGLTCPYNEDDRRCMSIFKGGTIGYNRTYFSEWAKNVLDEVKMRKMDEAAQATTNH 451

Query: 121 VHECFCN 127
           VHEC CN
Sbjct: 452 VHECSCN 458

[Close]

BLAST of Moc10g03480 vs. NCBI nr
Match: XP_022942991.1 (uncharacterized protein LOC111447859 [Cucurbita moschata])

HSP 1 Score: 232.6 bits (592), Expect = 1.8e-57
Identity = 105/127 (82.68%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTN+FLMDRNSSVMEFFPKGWLKLAGIGQFV++W+ASWSGM HQG 
Sbjct: 333 MGKTDILVSPHGAQLTNMFLMDRNSSVMEFFPKGWLKLAGIGQFVYQWMASWSGMRHQGA 392

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAA-GSANH 120
           WRDP+G  CPY E DRRCMS++K GTIGYNRT FSEWAKNVLNEVK RKM+EAA  +ANH
Sbjct: 393 WRDPNGLTCPYNEDDRRCMSIFKGGTIGYNRTYFSEWAKNVLNEVKTRKMDEAAQATANH 452

Query: 121 VHECFCN 127
           VH+C CN
Sbjct: 453 VHQCSCN 459

[Close]

BLAST of Moc10g03480 vs. NCBI nr
Match: XP_038905835.1 (uncharacterized protein LOC120091787 [Benincasa hispida])

HSP 1 Score: 228.0 bits (580), Expect = 4.5e-56
Identity = 103/126 (81.75%), Postives = 111/126 (88.10%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQ+TN+ LM+RNSSVMEFFPKGWLKLAGIGQ+V+ W+ASWSGM HQG 
Sbjct: 1   MGKTDILVSPHGAQMTNMILMNRNSSVMEFFPKGWLKLAGIGQYVYHWIASWSGMRHQGA 60

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAG-SANH 120
           WRDPHGP CPY   DRRCMS+YKSGTIGYNRT FSEWAKNVLNEVKMRK+EEA    AN 
Sbjct: 61  WRDPHGPPCPYAPDDRRCMSIYKSGTIGYNRTHFSEWAKNVLNEVKMRKIEEATQVPANK 120

Query: 121 VHECFC 126
           VHEC C
Sbjct: 121 VHECSC 126

[Close]

BLAST of Moc10g03480 vs. ExPASy TrEMBL
Match: A0A6J1CHS3 (uncharacterized protein LOC111011532 OS=Momordica charantia OX=3673 GN=LOC111011532 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.1e-71
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD
Sbjct: 316 MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 375

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHV 120
           WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHV
Sbjct: 376 WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANHV 435

Query: 121 HECFCN 127
           HECFCN
Sbjct: 436 HECFCN 441

[Close]

BLAST of Moc10g03480 vs. ExPASy TrEMBL
Match: A0A6J1J255 (uncharacterized protein LOC111482727 OS=Cucurbita maxima OX=3661 GN=LOC111482727 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.1e-59
Identity = 107/127 (84.25%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTN+FLMDRNSSVMEFFPKGWLKLAGIGQFV++W+ASWSGM HQG 
Sbjct: 333 MGKTDILVSPHGAQLTNMFLMDRNSSVMEFFPKGWLKLAGIGQFVYQWMASWSGMRHQGA 392

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAA-GSANH 120
           WRDPHG  CPY E DRRCMS++K GTIGYNRT FSEWAKNVLNEVK+RKM EAA  +ANH
Sbjct: 393 WRDPHGLTCPYNEDDRRCMSIFKGGTIGYNRTYFSEWAKNVLNEVKIRKMNEAAHATANH 452

Query: 121 VHECFCN 127
           VHEC CN
Sbjct: 453 VHECSCN 459

[Close]

BLAST of Moc10g03480 vs. ExPASy TrEMBL
Match: A0A6J1FQH3 (uncharacterized protein LOC111447859 OS=Cucurbita moschata OX=3662 GN=LOC111447859 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.8e-58
Identity = 105/127 (82.68%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDILVSPHGAQLTN+FLMDRNSSVMEFFPKGWLKLAGIGQFV++W+ASWSGM HQG 
Sbjct: 333 MGKTDILVSPHGAQLTNMFLMDRNSSVMEFFPKGWLKLAGIGQFVYQWMASWSGMRHQGA 392

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAA-GSANH 120
           WRDP+G  CPY E DRRCMS++K GTIGYNRT FSEWAKNVLNEVK RKM+EAA  +ANH
Sbjct: 393 WRDPNGLTCPYNEDDRRCMSIFKGGTIGYNRTYFSEWAKNVLNEVKTRKMDEAAQATANH 452

Query: 121 VHECFCN 127
           VH+C CN
Sbjct: 453 VHQCSCN 459

[Close]

BLAST of Moc10g03480 vs. ExPASy TrEMBL
Match: A0A0A0KXZ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G112630 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.2e-51
Identity = 97/127 (76.38%), Postives = 109/127 (85.83%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           MGKTDIL+SPHGAQLTN+ LM+RNSSVMEFFPKGWL+LAGIGQ+V+ WLASWSGM HQG 
Sbjct: 330 MGKTDILISPHGAQLTNMILMNRNSSVMEFFPKGWLELAGIGQYVYHWLASWSGMRHQGA 389

Query: 61  WRDPHGP-ACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAG-SAN 120
           WRDP+    CPY   DRRCMS+YK+GTIGYNRT FSEWAK+VLNEVKMRKMEEA   + N
Sbjct: 390 WRDPNSTLPCPYSPGDRRCMSIYKAGTIGYNRTHFSEWAKSVLNEVKMRKMEEATKVTTN 449

Query: 121 HVHECFC 126
            +HEC C
Sbjct: 450 QIHECSC 456

[Close]

BLAST of Moc10g03480 vs. ExPASy TrEMBL
Match: A0A5J5BRR5 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_020423 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.2e-51
Identity = 92/115 (80.00%), Postives = 103/115 (89.57%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           M  TDILVSPHGAQLTN+F MDRNSSVMEFFPKGWLKLAG+GQ+V+ W+ASWSGM HQG 
Sbjct: 376 MSSTDILVSPHGAQLTNMFFMDRNSSVMEFFPKGWLKLAGVGQYVYHWIASWSGMRHQGA 435

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAG 116
           WRDP+G  CPYP+ DRRCMS+YKSG IGYN T F+EWA+NVLNEVKMRKMEEA+G
Sbjct: 436 WRDPNGDHCPYPDDDRRCMSIYKSGKIGYNETYFAEWARNVLNEVKMRKMEEASG 490

[Close]

BLAST of Moc10g03480 vs. TAIR 10
Match: AT4G33590.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G33600.1); Has 126 Blast hits to 126 proteins in 35 species: Archae - 0; Bacteria - 12; Metazoa - 0; Fungi - 21; Plants - 62; Viruses - 0; Other Eukaryotes - 31 (source: NCBI BLink). )

HSP 1 Score: 181.4 bits (459), Expect = 4.5e-46
Identity = 80/119 (67.23%), Postives = 95/119 (79.83%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           M KTD+LVSPHGAQLTNLFLMD+NSSVMEFFPKGWLKLAG+GQ VF+W A+WSGM H+G 
Sbjct: 339 MKKTDVLVSPHGAQLTNLFLMDKNSSVMEFFPKGWLKLAGVGQLVFQWGANWSGMRHEGS 398

Query: 61  WRDPHGPACPYPEHDRRCMSVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAGSANH 120
           W DP G  C +P+ DRRCMS+YK+  IGYN T F EWA+ VL +  +R+M+E A   NH
Sbjct: 399 WHDPVGEICQFPDTDRRCMSIYKNAMIGYNETYFGEWARRVLGKFSIREMKELA-ECNH 456

[Close]

BLAST of Moc10g03480 vs. TAIR 10
Match: AT4G33600.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: 4 anthesis, C globular stage, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G33590.1); Has 131 Blast hits to 131 proteins in 40 species: Archae - 0; Bacteria - 9; Metazoa - 12; Fungi - 24; Plants - 58; Viruses - 0; Other Eukaryotes - 28 (source: NCBI BLink). )

HSP 1 Score: 171.4 bits (433), Expect = 4.6e-43
Identity = 80/129 (62.02%), Postives = 92/129 (71.32%), Query Frame = 0


Query: 1   MGKTDILVSPHGAQLTNLFLMDRNSSVMEFFPKGWLKLAGIGQFVFRWLASWSGMTHQGD 60
           M  TD+LVSPHGAQLTNL LMDRNSSVMEF PKGW KLAG+GQ V++W   WSGM H+G 
Sbjct: 342 MRMTDVLVSPHGAQLTNLVLMDRNSSVMEFLPKGWRKLAGVGQLVYQWGTRWSGMRHEGS 401

Query: 61  WRDPHGPACPYPEHDRRCM-SVYKSGTIGYNRTQFSEWAKNVLNEVKMRKMEEAAG---S 120
           W DP G  C +P+ DRRCM SVYK+G IGYN T F EWAK+VL + K RKM    G   S
Sbjct: 402 WHDPDGEICQFPDTDRRCMSSVYKNGRIGYNETYFGEWAKSVLGKFKERKMANVVGRKHS 461

Query: 121 ANHVHECFC 126
              +  C+C
Sbjct: 462 YGSLDGCWC 470

The following BLAST results are available for this feature:

BLAST of Moc10g03480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141026.1	2.2e-71	100.00	uncharacterized protein LOC111011532 [Momordica charantia]	[more]
XP_022984427.1	1.3e-58	84.25	uncharacterized protein LOC111482727 [Cucurbita maxima]	[more]
XP_023541716.1	2.8e-58	83.46	uncharacterized protein LOC111801789 [Cucurbita pepo subsp. pepo]	[more]
XP_022942991.1	1.8e-57	82.68	uncharacterized protein LOC111447859 [Cucurbita moschata]	[more]
XP_038905835.1	4.5e-56	81.75	uncharacterized protein LOC120091787 [Benincasa hispida]	[more]

BLAST of Moc10g03480 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc10g03480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHS3	1.1e-71	100.00	uncharacterized protein LOC111011532 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1J255	6.1e-59	84.25	uncharacterized protein LOC111482727 OS=Cucurbita maxima OX=3661 GN=LOC111482727...	[more]
A0A6J1FQH3	8.8e-58	82.68	uncharacterized protein LOC111447859 OS=Cucurbita moschata OX=3662 GN=LOC1114478...	[more]
A0A0A0KXZ9	1.2e-51	76.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G112630 PE=4 SV=1	[more]
A0A5J5BRR5	1.2e-51	80.00	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_020423 PE=4 SV=1	[more]

BLAST of Moc10g03480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33590.1	4.5e-46	67.23	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT4G33600.1	4.6e-43	62.02	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007657	Glycosyltransferase 61	PFAM	PF04577	DUF563	coord: 3..57 e-value: 6.7E-9 score: 36.1
IPR007657	Glycosyltransferase 61	PANTHER	PTHR20961	GLYCOSYLTRANSFERASE	coord: 1..118
None	No IPR available	PANTHER	PTHR20961:SF94	TRANSMEMBRANE PROTEIN	coord: 1..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g03480.1	Moc10g03480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016763	pentosyltransferase activity
molecular_function	GO:0016757	glycosyltransferase activity