Moc10g03470 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g03470
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr10: 2471764 .. 2472478 (+)
RNA-Seq Expression	Moc10g03470
Synteny	Moc10g03470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGAAGAATACTGCAAAACAACCATTGAAATTGCGAGAGAATTGGTGGGAGGAATCTCAGAGAGCCTGGGGTTGGAAACCTGTAATCTAGAGAGAGCAGTGGATTTTGAATCGTGCTCAGTTTTGTTTGCAGCAAACTTTTATCCGCCATGTCCACAGCCAGAGCTTGCAATGGGTTTGCCATCTCACTCTGATCATTGCCTTCTCACCCTTCTTCTTCAGAACCAAATTTCTGGCCTTCAAATCTTGCATCAAGGCAACTGGGTGAATGTCAATCCCATTCCCAATTCATTTCTAGTTCATGTAGCTGACCAACTAGAGGTACAAATCCATTTTTCCTTTTTCCTTTTTTTTAGTTAAGGTAGAAAGATTTAAATCTCCGACCTTTCCGGAGGTAGGTAAGTAATAATTGATGAGTTGAATTTGTTTTTGGGTGTGGTAGATCTTGAGCAATGGTAAGTACAAGAGCGTTTTGCACAGAGCAACGGTGAACAATAAGGCCACAAGGTTGTCTATAGTCATGGCAGTTCGGCCATCACCTGAAACAGTGGTTGGTCCTGCACCTCAATTGCTGAATAAACTCACCAATCCTCCTTTGCTTAAGCACATCAAATACATAGATTATATGCAAATGGTGTATAGTAGCAATCTCCAAGATCCAACGCATTTGTCTACTTTGAATCTCGATCTTTATCGTATGTGTTTTTATTAG

mRNA sequence

ATGTTGGAAGAATACTGCAAAACAACCATTGAAATTGCGAGAGAATTGGTGGGAGGAATCTCAGAGAGCCTGGGGTTGGAAACCTGTAATCTAGAGAGAGCAGTGGATTTTGAATCGTGCTCAGTTTTGTTTGCAGCAAACTTTTATCCGCCATGTCCACAGCCAGAGCTTGCAATGGGTTTGCCATCTCACTCTGATCATTGCCTTCTCACCCTTCTTCTTCAGAACCAAATTTCTGGCCTTCAAATCTTGCATCAAGGCAACTGGGTGAATGTCAATCCCATTCCCAATTCATTTCTAGTTCATGTAGCTGACCAACTAGAGATCTTGAGCAATGGTAAGTACAAGAGCGTTTTGCACAGAGCAACGGTGAACAATAAGGCCACAAGGTTGTCTATAGTCATGGCAGTTCGGCCATCACCTGAAACAGTGGTTGGTCCTGCACCTCAATTGCTGAATAAACTCACCAATCCTCCTTTGCTTAAGCACATCAAATACATAGATTATATGCAAATGGTGTATAGTAGCAATCTCCAAGATCCAACGCATTTGTCTACTTTGAATCTCGATCTTTATCGTATGTGTTTTTATTAG

Coding sequence (CDS)

ATGTTGGAAGAATACTGCAAAACAACCATTGAAATTGCGAGAGAATTGGTGGGAGGAATCTCAGAGAGCCTGGGGTTGGAAACCTGTAATCTAGAGAGAGCAGTGGATTTTGAATCGTGCTCAGTTTTGTTTGCAGCAAACTTTTATCCGCCATGTCCACAGCCAGAGCTTGCAATGGGTTTGCCATCTCACTCTGATCATTGCCTTCTCACCCTTCTTCTTCAGAACCAAATTTCTGGCCTTCAAATCTTGCATCAAGGCAACTGGGTGAATGTCAATCCCATTCCCAATTCATTTCTAGTTCATGTAGCTGACCAACTAGAGATCTTGAGCAATGGTAAGTACAAGAGCGTTTTGCACAGAGCAACGGTGAACAATAAGGCCACAAGGTTGTCTATAGTCATGGCAGTTCGGCCATCACCTGAAACAGTGGTTGGTCCTGCACCTCAATTGCTGAATAAACTCACCAATCCTCCTTTGCTTAAGCACATCAAATACATAGATTATATGCAAATGGTGTATAGTAGCAATCTCCAAGATCCAACGCATTTGTCTACTTTGAATCTCGATCTTTATCGTATGTGTTTTTATTAG

Protein sequence

MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQDPTHLSTLNLDLYRMCFY

Homology

[Close]

BLAST of Moc10g03470 vs. NCBI nr
Match: XP_022141030.1 (flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia])

HSP 1 Score: 400.2 bits (1027), Expect = 1.0e-107
Identity = 196/197 (99.49%), Postives = 196/197 (99.49%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG
Sbjct: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSDHCLLTLLL NQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH
Sbjct: 61  LPSHSDHCLLTLLLHNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD 180
           RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD
Sbjct: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD 180

Query: 181 PTHLSTLNLDLYRMCFY 198
           PTHLSTLNLDLYRMCFY
Sbjct: 181 PTHLSTLNLDLYRMCFY 197

[Close]

BLAST of Moc10g03470 vs. NCBI nr
Match: XP_022141027.1 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_022159285.1 protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 318.2 bits (814), Expect = 5.1e-83
Identity = 159/180 (88.33%), Postives = 166/180 (92.22%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           +LEEYCK +IE+ARELV GISESLGLETC+LERAVDFESCS LFAANFYPP PQPELA G
Sbjct: 160 ILEEYCKRSIEMARELVRGISESLGLETCHLERAVDFESCSTLFAANFYPPYPQPELARG 219

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSD CLLTLLLQNQISGLQILHQGNWV+VNPIPNSFLV+VADQLEILSNGKYKSVLH
Sbjct: 220 LPSHSDQCLLTLLLQNQISGLQILHQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLH 279

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD 180
           RA VNNKATRLSI +AV  SPETVV PAPQLLNKLTNPPL KHIKY DYMQMV SSNLQD
Sbjct: 280 RAMVNNKATRLSIAVAVGSSPETVVSPAPQLLNKLTNPPLFKHIKYTDYMQMVQSSNLQD 339

[Close]

BLAST of Moc10g03470 vs. NCBI nr
Match: XP_038904929.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 280.0 bits (715), Expect = 1.5e-71
Identity = 140/192 (72.92%), Postives = 160/192 (83.33%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           +LEEYCK   E+ RELV GISESLGLE C L++A + ESCS++FAAN YPPCPQPELA G
Sbjct: 159 ILEEYCKRIREMTRELVRGISESLGLEECCLDKAANLESCSIVFAANLYPPCPQPELARG 218

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSDHCLLT+LLQNQI+GLQILH   W+NVNPIPNS LV+VADQLEILSNGKYKSVLH
Sbjct: 219 LPSHSDHCLLTILLQNQIAGLQILHHDKWLNVNPIPNSLLVNVADQLEILSNGKYKSVLH 278

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLT-NPPLLKHIKYIDYMQMVYSSNLQ 180
           RA VN+KATR+SI MAV PS ETVVGPAPQL+NK T NPPL K+IKYIDYM +V S+ LQ
Sbjct: 279 RAIVNDKATRISIAMAVGPSLETVVGPAPQLINKHTNNPPLFKNIKYIDYMGIVQSNKLQ 338

Query: 181 DPTHLSTLNLDL 192
             + L  + L +
Sbjct: 339 GKSTLDRIRLHI 350

[Close]

BLAST of Moc10g03470 vs. NCBI nr
Match: XP_022149149.1 (protein DMR6-LIKE OXYGENASE 1-like [Momordica charantia])

HSP 1 Score: 266.2 bits (679), Expect = 2.3e-67
Identity = 126/187 (67.38%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEYC+   E+AREL  GISESLGLE C LE+AV+ +SCS +   N YPPCPQ E+AMGLP
Sbjct: 186 EEYCRRVRELARELARGISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLP 245

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
            HSDHCLLT +LQN+I GLQILHQG WVNVNPIPNSFLV+ ADQLEI SNGKYKS+LHRA
Sbjct: 246 PHSDHCLLTFILQNKICGLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRA 305

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQDPT 182
            VN KATR+S+ +A+ PS +TVVGPAP+L+++LTNPPL KHIKY DYM++V +++LQ  +
Sbjct: 306 IVNKKATRMSVGIAIGPSLDTVVGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKS 365

Query: 183 HLSTLNL 190
            L  + L
Sbjct: 366 MLDLVRL 372

[Close]

BLAST of Moc10g03470 vs. NCBI nr
Match: XP_004137271.1 (protein DMR6-LIKE OXYGENASE 2 [Cucumis sativus] >KGN53731.1 hypothetical protein Csa_015236 [Cucumis sativus])

HSP 1 Score: 261.2 bits (666), Expect = 7.4e-66
Identity = 132/192 (68.75%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           +LEEYC +  E+ REL+ GISESLGLE C LE+A D ES  +LFAAN YPPCPQPELA G
Sbjct: 159 ILEEYCTSVREMTRELLRGISESLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARG 218

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSD CLLT+LL NQI+GLQILH   W NVNPIPNSF+++V DQLEILSNGKY+SVLH
Sbjct: 219 LPSHSDLCLLTILLTNQIAGLQILHHDKWFNVNPIPNSFIINVGDQLEILSNGKYESVLH 278

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLT-NPPLLKHIKYIDYMQMVYSSNLQ 180
           RA VN+KATR+SI MAV PS ETVVGPAPQL+N+ T NPP+ K IKY DYM+++ SS LQ
Sbjct: 279 RAKVNDKATRISIGMAVGPSHETVVGPAPQLVNEDTNNPPMFKSIKYKDYMEIMQSSQLQ 338

Query: 181 DPTHLSTLNLDL 192
           + + L    L L
Sbjct: 339 EKSILDRFRLHL 350

[Close]

BLAST of Moc10g03470 vs. ExPASy Swiss-Prot
Match: Q6Z244 (2-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD19 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.2e-36
Identity = 82/187 (43.85%), Postives = 111/187 (59.36%), Query Frame = 0


Query: 4   EYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQP-ELAMGLP 63
           EY   T  +  EL   ISESLGL    L  A++ ESC  +   N YP C +P E AMGL 
Sbjct: 147 EYATCTRALLLELTKAISESLGLAGGRLSEALNLESCFQILVGNHYPACSRPDEQAMGLS 206

Query: 64  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 123
           +HSDH LLTLL QN + GLQ+ H G W+   P+P SF V   DQLEI++NG+YK VLHRA
Sbjct: 207 AHSDHGLLTLLFQNGVDGLQVKHDGEWLLAKPLPGSFFVIAGDQLEIVTNGRYKGVLHRA 266

Query: 124 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQDPT 183
            V  + +R+S V  + P  +TVV P P++          + I+Y DYM+M  S+++ + T
Sbjct: 267 VVGGEQSRMSFVSLIGPCMDTVVEPLPEMAADGRGLE-FRGIRYRDYMEMQQSNSINEKT 326

Query: 184 HLSTLNL 190
            L  + +
Sbjct: 327 ALDIVRV 332

[Close]

BLAST of Moc10g03470 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.9e-36
Identity = 73/175 (41.71%), Postives = 114/175 (65.14%), Query Frame = 0


Query: 4   EYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLPS 63
           EY  +   +   L+  ISESLGLE+ ++   +   +  + F  N+YPPCP+PEL  GLP 
Sbjct: 163 EYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAF--NYYPPCPEPELTYGLPG 222

Query: 64  HSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRAT 123
           H D  ++T+LLQ+Q+SGLQ+     WV V+PIPN+F+V++ DQ++++SN KYKSVLHRA 
Sbjct: 223 HKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAV 282

Query: 124 VNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNL 179
           VN +  RLSI     PS + V+GPA +L+N+  +  + +   +++Y    ++ +L
Sbjct: 283 VNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDKFWNRSL 335

[Close]

BLAST of Moc10g03470 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.4e-36
Identity = 75/168 (44.64%), Postives = 106/168 (63.10%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEY K   E+   +   +S  LGLE   +  A   +    L   N+YPPCP+P+LA+G+ 
Sbjct: 173 EEYGKRMREVVDRIFKSLSLGLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVV 232

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
           +H+D   +T+L+ N++ GLQ+   G+W +V  IPN+ +VH+ DQ+EILSNGKYKSV HR 
Sbjct: 233 AHTDMSYITILVPNEVQGLQVFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRT 292

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYM 171
           TVN   TR+S  + + P  E  VGP P+LL++  NPP  K  KY DY+
Sbjct: 293 TVNKDKTRMSWPVFLEPPSEHEVGPIPKLLSE-ANPPKFKTKKYKDYV 339

[Close]

BLAST of Moc10g03470 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.9e-35
Identity = 73/167 (43.71%), Postives = 107/167 (64.07%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEY K   E+  +L   +S  LG+E   L+ A   +    +   N+YPPCP+P+LA+G+ 
Sbjct: 160 EEYAKYMREVVDKLFTYLSLGLGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVV 219

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
           +H+D   LT+L+ N++ GLQ+     W++   IPN+ ++H+ DQ+EILSNGKYK+VLHR 
Sbjct: 220 AHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRT 279

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDY 170
           TVN   TR+S  + + P  +TVVGP PQL++   NPP  K  K+ DY
Sbjct: 280 TVNKDKTRMSWPVFLEPPADTVVGPLPQLVDD-ENPPKYKAKKFKDY 325

[Close]

BLAST of Moc10g03470 vs. ExPASy Swiss-Prot
Match: Q41452 (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.1e-35
Identity = 76/168 (45.24%), Postives = 106/168 (63.10%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEY K   ++A  +   +S  LGLE   +  A   E    +   N+YPPCP+P+LA+G+ 
Sbjct: 177 EEYAKWLRKVADGIFRSLSLGLGLEGHEMMEAAGSEDIVYMLKINYYPPCPRPDLALGVV 236

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
           +H+D   +TLL+ N++   Q+   G+W +VN IPN+ +VH+ DQ+EILSNGKYKSV HR 
Sbjct: 237 AHTDMSYITLLVPNEV---QVFKDGHWYDVNYIPNAIIVHIGDQVEILSNGKYKSVYHRT 296

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYM 171
           TVN   TR+S  + + PS E  VGP P L+N+  NPP  K  KY DY+
Sbjct: 297 TVNKYKTRMSWPVFLEPSSEHEVGPIPNLINE-ANPPKFKTKKYKDYV 340

[Close]

BLAST of Moc10g03470 vs. ExPASy TrEMBL
Match: A0A6J1CGT6 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111011535 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 5.0e-108
Identity = 196/197 (99.49%), Postives = 196/197 (99.49%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG
Sbjct: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSDHCLLTLLL NQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH
Sbjct: 61  LPSHSDHCLLTLLLHNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD 180
           RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD
Sbjct: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD 180

Query: 181 PTHLSTLNLDLYRMCFY 198
           PTHLSTLNLDLYRMCFY
Sbjct: 181 PTHLSTLNLDLYRMCFY 197

[Close]

BLAST of Moc10g03470 vs. ExPASy TrEMBL
Match: A0A6J1CIQ0 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011533 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.5e-83
Identity = 159/180 (88.33%), Postives = 166/180 (92.22%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           +LEEYCK +IE+ARELV GISESLGLETC+LERAVDFESCS LFAANFYPP PQPELA G
Sbjct: 160 ILEEYCKRSIEMARELVRGISESLGLETCHLERAVDFESCSTLFAANFYPPYPQPELARG 219

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSD CLLTLLLQNQISGLQILHQGNWV+VNPIPNSFLV+VADQLEILSNGKYKSVLH
Sbjct: 220 LPSHSDQCLLTLLLQNQISGLQILHQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLH 279

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQD 180
           RA VNNKATRLSI +AV  SPETVV PAPQLLNKLTNPPL KHIKY DYMQMV SSNLQD
Sbjct: 280 RAMVNNKATRLSIAVAVGSSPETVVSPAPQLLNKLTNPPLFKHIKYTDYMQMVQSSNLQD 339

[Close]

BLAST of Moc10g03470 vs. ExPASy TrEMBL
Match: A0A6J1D661 (protein DMR6-LIKE OXYGENASE 1-like OS=Momordica charantia OX=3673 GN=LOC111017638 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.1e-67
Identity = 126/187 (67.38%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEYC+   E+AREL  GISESLGLE C LE+AV+ +SCS +   N YPPCPQ E+AMGLP
Sbjct: 186 EEYCRRVRELARELARGISESLGLEGCTLEKAVNLKSCSQILVGNLYPPCPQAEVAMGLP 245

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
            HSDHCLLT +LQN+I GLQILHQG WVNVNPIPNSFLV+ ADQLEI SNGKYKS+LHRA
Sbjct: 246 PHSDHCLLTFILQNKICGLQILHQGKWVNVNPIPNSFLVNTADQLEIFSNGKYKSLLHRA 305

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNLQDPT 182
            VN KATR+S+ +A+ PS +TVVGPAP+L+++LTNPPL KHIKY DYM++V +++LQ  +
Sbjct: 306 IVNKKATRMSVGIAIGPSLDTVVGPAPELVHQLTNPPLYKHIKYKDYMELVQTNDLQHKS 365

Query: 183 HLSTLNL 190
            L  + L
Sbjct: 366 MLDLVRL 372

[Close]

BLAST of Moc10g03470 vs. ExPASy TrEMBL
Match: A0A0A0L0R1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G112650 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.6e-66
Identity = 132/192 (68.75%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           +LEEYC +  E+ REL+ GISESLGLE C LE+A D ES  +LFAAN YPPCPQPELA G
Sbjct: 159 ILEEYCTSVREMTRELLRGISESLGLEGCFLEKATDLESSLILFAANLYPPCPQPELARG 218

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSD CLLT+LL NQI+GLQILH   W NVNPIPNSF+++V DQLEILSNGKY+SVLH
Sbjct: 219 LPSHSDLCLLTILLTNQIAGLQILHHDKWFNVNPIPNSFIINVGDQLEILSNGKYESVLH 278

Query: 121 RATVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLT-NPPLLKHIKYIDYMQMVYSSNLQ 180
           RA VN+KATR+SI MAV PS ETVVGPAPQL+N+ T NPP+ K IKY DYM+++ SS LQ
Sbjct: 279 RAKVNDKATRISIGMAVGPSHETVVGPAPQLVNEDTNNPPMFKSIKYKDYMEIMQSSQLQ 338

Query: 181 DPTHLSTLNLDL 192
           + + L    L L
Sbjct: 339 EKSILDRFRLHL 350

[Close]

BLAST of Moc10g03470 vs. ExPASy TrEMBL
Match: A0A6J1E3G6 (protein DMR6-LIKE OXYGENASE 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025698 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.0e-64
Identity = 127/145 (87.59%), Postives = 135/145 (93.10%), Query Frame = 0


Query: 1   MLEEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMG 60
           +LEEYCK +IE+ARELV GISESLGLETC+LERAVDFESCS LFAANFYPP PQPELA G
Sbjct: 160 ILEEYCKRSIEMARELVRGISESLGLETCHLERAVDFESCSTLFAANFYPPYPQPELARG 219

Query: 61  LPSHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLH 120
           LPSHSD CLLTLLLQNQISGLQILHQGNWV+VNPIPNSFLV+VADQLEILSNGKYKSVLH
Sbjct: 220 LPSHSDQCLLTLLLQNQISGLQILHQGNWVDVNPIPNSFLVNVADQLEILSNGKYKSVLH 279

Query: 121 RATVNNKATRLSIVMAVRPSPETVV 146
           RA VNNKATRLSI +AV  SPETV+
Sbjct: 280 RAMVNNKATRLSIAVAVGSSPETVI 304

[Close]

BLAST of Moc10g03470 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 3.5e-37
Identity = 73/175 (41.71%), Postives = 114/175 (65.14%), Query Frame = 0


Query: 4   EYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLPS 63
           EY  +   +   L+  ISESLGLE+ ++   +   +  + F  N+YPPCP+PEL  GLP 
Sbjct: 163 EYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAF--NYYPPCPEPELTYGLPG 222

Query: 64  HSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRAT 123
           H D  ++T+LLQ+Q+SGLQ+     WV V+PIPN+F+V++ DQ++++SN KYKSVLHRA 
Sbjct: 223 HKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAV 282

Query: 124 VNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYSSNL 179
           VN +  RLSI     PS + V+GPA +L+N+  +  + +   +++Y    ++ +L
Sbjct: 283 VNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDKFWNRSL 335

[Close]

BLAST of Moc10g03470 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 146.0 bits (367), Expect = 3.3e-35
Identity = 71/174 (40.80%), Postives = 109/174 (62.64%), Query Frame = 0


Query: 4   EYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLPS 63
           EY  +   +   L+  ISESLGL    +   +      +  A N+YP CPQPEL  GLP 
Sbjct: 161 EYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHM--AINYYPRCPQPELTYGLPG 220

Query: 64  HSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRAT 123
           H D  L+T+LLQ+++SGLQ+   G W+ VNP+PN+F+V++ DQ++++SN KYKSVLHRA 
Sbjct: 221 HKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAV 280

Query: 124 VNNKATRLSIVMAVRPSPETVVGPAPQLLN-KLTNPPLLKHIKYIDYMQMVYSS 177
           VN+   R+SI     PS + V+ PA +L+N +  +P + ++  Y +Y +  + +
Sbjct: 281 VNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDT 332

[Close]

BLAST of Moc10g03470 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 143.3 bits (360), Expect = 2.1e-34
Identity = 76/171 (44.44%), Postives = 108/171 (63.16%), Query Frame = 0


Query: 5   YCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLPSH 64
           Y K T E+   +V  I ESL ++  + E A + E  S +   N YPPCP+PEL +G+P H
Sbjct: 181 YAKETKEMFEMMVKAILESLEIDGSD-EAAKELEEGSQVVVVNCYPPCPEPELTLGMPPH 240

Query: 65  SDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRATV 124
           SD+  LTLLLQ+++ GLQIL++  WV V+PIP SF+V+V D LEI SNG+YKSVLHR  V
Sbjct: 241 SDYGFLTLLLQDEVEGLQILYRDEWVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLV 300

Query: 125 NNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDYMQMVYS 176
           N+   R+S+         +VV P+P+L++K  NP       +  ++Q + S
Sbjct: 301 NSTKPRISVASLHSFPLTSVVKPSPKLVDK-HNPSQYMDTDFTTFLQYITS 349

[Close]

BLAST of Moc10g03470 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 139.4 bits (350), Expect = 3.0e-33
Identity = 66/167 (39.52%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEY     +++  L+G +S+ LGL+   L+  +  E    +   N+YPPCP+P+LA+G+P
Sbjct: 160 EEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVP 219

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
           +H+D   +TLL+ N++ GLQ+    +W +   IP++ +VH+ DQ+  LSNG+YK+VLHR 
Sbjct: 220 AHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRT 279

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDY 170
           TV+ + TR+S  + + P  E +VGP P+L     NPP  K   + DY
Sbjct: 280 TVDKEKTRMSWPVFLEPPREKIVGPLPELTGD-DNPPKFKPFAFKDY 325

[Close]

BLAST of Moc10g03470 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 139.4 bits (350), Expect = 3.0e-33
Identity = 66/167 (39.52%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 3   EEYCKTTIEIARELVGGISESLGLETCNLERAVDFESCSVLFAANFYPPCPQPELAMGLP 62
           EEY     +++  L+G +S+ LGL+   L+  +  E    +   N+YPPCP+P+LA+G+P
Sbjct: 160 EEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVP 219

Query: 63  SHSDHCLLTLLLQNQISGLQILHQGNWVNVNPIPNSFLVHVADQLEILSNGKYKSVLHRA 122
           +H+D   +TLL+ N++ GLQ+    +W +   IP++ +VH+ DQ+  LSNG+YK+VLHR 
Sbjct: 220 AHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRT 279

Query: 123 TVNNKATRLSIVMAVRPSPETVVGPAPQLLNKLTNPPLLKHIKYIDY 170
           TV+ + TR+S  + + P  E +VGP P+L     NPP  K   + DY
Sbjct: 280 TVDKEKTRMSWPVFLEPPREKIVGPLPELTGD-DNPPKFKPFAFKDY 325

The following BLAST results are available for this feature:

BLAST of Moc10g03470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141030.1	1.0e-107	99.49	flavonol synthase/flavanone 3-hydroxylase-like [Momordica charantia]	[more]
XP_022141027.1	5.1e-83	88.33	protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_02215928...	[more]
XP_038904929.1	1.5e-71	72.92	protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida]	[more]
XP_022149149.1	2.3e-67	67.38	protein DMR6-LIKE OXYGENASE 1-like [Momordica charantia]	[more]
XP_004137271.1	7.4e-66	68.75	protein DMR6-LIKE OXYGENASE 2 [Cucumis sativus] >KGN53731.1 hypothetical protein...	[more]

BLAST of Moc10g03470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Z244	2.2e-36	43.85	2-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9ZSA8	4.9e-36	41.71	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]
Q07512	6.4e-36	44.64	Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ...	[more]
Q9ZWQ9	1.9e-35	43.71	Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ...	[more]
Q41452	4.1e-35	45.24	Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]

BLAST of Moc10g03470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGT6	5.0e-108	99.49	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CIQ0	2.5e-83	88.33	protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1D661	1.1e-67	67.38	protein DMR6-LIKE OXYGENASE 1-like OS=Momordica charantia OX=3673 GN=LOC11101763...	[more]
A0A0A0L0R1	3.6e-66	68.75	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A6J1E3G6	2.0e-64	87.59	protein DMR6-LIKE OXYGENASE 1-like isoform X2 OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of Moc10g03470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10500.1	3.5e-37	41.71	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	3.3e-35	40.80	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G36690.1	2.1e-34	44.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G08640.1	3.0e-33	39.52	flavonol synthase 1	[more]
AT5G08640.2	3.0e-33	39.52	flavonol synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..189 e-value: 5.1E-55 score: 189.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 44..136 e-value: 1.4E-24 score: 86.5
None	No IPR available	PANTHER	PTHR47991:SF61	PROTEIN DMR6-LIKE OXYGENASE 2-LIKE	coord: 2..179
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 2..179
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..179
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 36..139 score: 13.334182

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g03470.1	Moc10g03470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity