Moc10g03390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g03390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Fe2OG dioxygenase domain-containing protein
Location	chr10: 2376547 .. 2377063 (-)
RNA-Seq Expression	Moc10g03390
Synteny	Moc10g03390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTCCACCGGCAATGGCCAGCATCAAAACCATAGCTGAAACACTCAACTTTGCCTCCATTCCTTCCTCTTGCATCTTCTCCGGCGACGGTGGCGCTGTCGATGCAGCTCCACAAGGTGTTGGAGATTCGATCCCCACCATTGATTTCTCTCTTCTCACCACGGGTACTCCCGATCAACGAGCCAAAGTCGTGGATCAGCTCGGCAAGGCATGCCAGGACTGGGGTTTCTTCATGGTACGTAGATGTTGGGGTAGAGCTAGAAATTTGGTAGGAATGATTAAAGCAATTTGGTTTTATAATTTTCACTTAATTTTTACTTCATCTTTCTCATCTCAAAGTTCTAATTTGATTTTAGCGTCCCATCTTTTACCCGCCCAGAAAATTCATCCATTTTTTTGTATCTAAGTGTCATGTATTCAATATGTTAATGTAGAGACAATTGGTGAGACTTGACCAGGCAAACGGAATCGAAGCTCTTAAAATTATAGAGGCCCAGTTGAAAACAAGCTCATGA

mRNA sequence

ATGATTCCACCGGCAATGGCCAGCATCAAAACCATAGCTGAAACACTCAACTTTGCCTCCATTCCTTCCTCTTGCATCTTCTCCGGCGACGGTGGCGCTGTCGATGCAGCTCCACAAGGTGTTGGAGATTCGATCCCCACCATTGATTTCTCTCTTCTCACCACGGGTACTCCCGATCAACGAGCCAAAGTCGTGGATCAGCTCGGCAAGGCATGCCAGGACTGGGGTTTCTTCATGAGACAATTGGTGAGACTTGACCAGGCAAACGGAATCGAAGCTCTTAAAATTATAGAGGCCCAGTTGAAAACAAGCTCATGA

Coding sequence (CDS)

ATGATTCCACCGGCAATGGCCAGCATCAAAACCATAGCTGAAACACTCAACTTTGCCTCCATTCCTTCCTCTTGCATCTTCTCCGGCGACGGTGGCGCTGTCGATGCAGCTCCACAAGGTGTTGGAGATTCGATCCCCACCATTGATTTCTCTCTTCTCACCACGGGTACTCCCGATCAACGAGCCAAAGTCGTGGATCAGCTCGGCAAGGCATGCCAGGACTGGGGTTTCTTCATGAGACAATTGGTGAGACTTGACCAGGCAAACGGAATCGAAGCTCTTAAAATTATAGAGGCCCAGTTGAAAACAAGCTCATGA

Protein sequence

MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQRAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS

Homology

[Close]

BLAST of Moc10g03390 vs. NCBI nr
Match: XP_022159287.1 (uncharacterized protein LOC111025699 [Momordica charantia])

HSP 1 Score: 207.2 bits (526), Expect = 6.8e-50
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 1   MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
           MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ
Sbjct: 1   MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60

Query: 61  RAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS 106
           RAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS
Sbjct: 61  RAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS 105

[Close]

BLAST of Moc10g03390 vs. NCBI nr
Match: XP_022149149.1 (protein DMR6-LIKE OXYGENASE 1-like [Momordica charantia])

HSP 1 Score: 134.0 bits (336), Expect = 7.3e-28
Identity = 65/79 (82.28%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1   MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
           M  PAMAS+KTIA+T N  SIPSS +FS DGGAVDAAPQGV DSIPT+DFSLLT GTPDQ
Sbjct: 25  MASPAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQ 84

Query: 61  RAKVVDQLGKACQDWGFFM 80
           RA+VVDQLGKACQDWGFFM
Sbjct: 85  RAEVVDQLGKACQDWGFFM 103

[Close]

BLAST of Moc10g03390 vs. NCBI nr
Match: XP_022141027.1 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_022159285.1 protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 127.5 bits (319), Expect = 6.8e-26
Identity = 63/79 (79.75%), Postives = 69/79 (87.34%), Query Frame = 0


Query: 1  MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
          M PPA+AS+KTIAET N ASIPSS IFS + GA  AAP+GV DSIPTIDFSLLT GTPDQ
Sbjct: 1  MAPPAVASVKTIAETPNLASIPSSYIFSANDGAAKAAPRGVEDSIPTIDFSLLTMGTPDQ 60

Query: 61 RAKVVDQLGKACQDWGFFM 80
          RAKVVD+LGKACQDWGFF+
Sbjct: 61 RAKVVDELGKACQDWGFFV 79

[Close]

BLAST of Moc10g03390 vs. NCBI nr
Match: XP_022141029.1 (protein DMR6-LIKE OXYGENASE 1-like isoform X2 [Momordica charantia] >XP_022159286.1 protein DMR6-LIKE OXYGENASE 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 127.5 bits (319), Expect = 6.8e-26
Identity = 63/79 (79.75%), Postives = 69/79 (87.34%), Query Frame = 0


Query: 1  MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
          M PPA+AS+KTIAET N ASIPSS IFS + GA  AAP+GV DSIPTIDFSLLT GTPDQ
Sbjct: 1  MAPPAVASVKTIAETPNLASIPSSYIFSANDGAAKAAPRGVEDSIPTIDFSLLTMGTPDQ 60

Query: 61 RAKVVDQLGKACQDWGFFM 80
          RAKVVD+LGKACQDWGFF+
Sbjct: 61 RAKVVDELGKACQDWGFFV 79

[Close]

BLAST of Moc10g03390 vs. NCBI nr
Match: XP_023552481.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 105.5 bits (262), Expect = 2.8e-19
Identity = 58/85 (68.24%), Postives = 64/85 (75.29%), Query Frame = 0


Query: 1   MIPPAMASIKTIAETLNFASIPSSCIF--SGDGGAV----DAAPQGVGDSIPTIDFSLLT 60
           + P A+ S+K IAET N ASIPSS IF  S D   V    DAAP GV DSIPTIDFSLLT
Sbjct: 24  LCPMALPSVKAIAETPNLASIPSSYIFTTSDDSDDVATTADAAPHGVEDSIPTIDFSLLT 83

Query: 61  TGTPDQRAKVVDQLGKACQDWGFFM 80
           TGTP QR+KVV++LGKAC DWGFFM
Sbjct: 84  TGTPHQRSKVVNELGKACHDWGFFM 108

[Close]

BLAST of Moc10g03390 vs. ExPASy Swiss-Prot
Match: Q6Z244 (2-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD19 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 5.3e-05
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 0


Query: 24 SCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQRAKVVDQLGKACQDWGFFM 80
          S   + DG A  +        IP +D  +L  G  D+R++ +  LG+AC+DWGFFM
Sbjct: 14 SAAAAADGSATPS------QGIPVVDLGVLINGAADERSRAIRDLGRACEDWGFFM 63

[Close]

BLAST of Moc10g03390 vs. ExPASy TrEMBL
Match: A0A6J1DZF0 (uncharacterized protein LOC111025699 OS=Momordica charantia OX=3673 GN=LOC111025699 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.3e-50
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 1   MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
           MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ
Sbjct: 1   MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60

Query: 61  RAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS 106
           RAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS
Sbjct: 61  RAKVVDQLGKACQDWGFFMRQLVRLDQANGIEALKIIEAQLKTSS 105

[Close]

BLAST of Moc10g03390 vs. ExPASy TrEMBL
Match: A0A6J1D661 (protein DMR6-LIKE OXYGENASE 1-like OS=Momordica charantia OX=3673 GN=LOC111017638 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.5e-28
Identity = 65/79 (82.28%), Postives = 70/79 (88.61%), Query Frame = 0


Query: 1   MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
           M  PAMAS+KTIA+T N  SIPSS +FS DGGAVDAAPQGV DSIPT+DFSLLT GTPDQ
Sbjct: 25  MASPAMASVKTIAQTPNLTSIPSSYVFSADGGAVDAAPQGVEDSIPTVDFSLLTMGTPDQ 84

Query: 61  RAKVVDQLGKACQDWGFFM 80
           RA+VVDQLGKACQDWGFFM
Sbjct: 85  RAEVVDQLGKACQDWGFFM 103

[Close]

BLAST of Moc10g03390 vs. ExPASy TrEMBL
Match: A0A6J1E3G6 (protein DMR6-LIKE OXYGENASE 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025698 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 3.3e-26
Identity = 63/79 (79.75%), Postives = 69/79 (87.34%), Query Frame = 0


Query: 1  MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
          M PPA+AS+KTIAET N ASIPSS IFS + GA  AAP+GV DSIPTIDFSLLT GTPDQ
Sbjct: 1  MAPPAVASVKTIAETPNLASIPSSYIFSANDGAAKAAPRGVEDSIPTIDFSLLTMGTPDQ 60

Query: 61 RAKVVDQLGKACQDWGFFM 80
          RAKVVD+LGKACQDWGFF+
Sbjct: 61 RAKVVDELGKACQDWGFFV 79

[Close]

BLAST of Moc10g03390 vs. ExPASy TrEMBL
Match: A0A6J1CIQ0 (protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011533 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 3.3e-26
Identity = 63/79 (79.75%), Postives = 69/79 (87.34%), Query Frame = 0


Query: 1  MIPPAMASIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQ 60
          M PPA+AS+KTIAET N ASIPSS IFS + GA  AAP+GV DSIPTIDFSLLT GTPDQ
Sbjct: 1  MAPPAVASVKTIAETPNLASIPSSYIFSANDGAAKAAPRGVEDSIPTIDFSLLTMGTPDQ 60

Query: 61 RAKVVDQLGKACQDWGFFM 80
          RAKVVD+LGKACQDWGFF+
Sbjct: 61 RAKVVDELGKACQDWGFFV 79

[Close]

BLAST of Moc10g03390 vs. ExPASy TrEMBL
Match: A0A5A7UYU4 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold753G00070 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-17
Identity = 50/78 (64.10%), Postives = 58/78 (74.36%), Query Frame = 0


Query: 6  MASIKTIAETLNFASIPSSCIFSGDGG----AVDAAPQGVGDSIPTIDFSLLTTGTPDQR 65
          MAS+K+IA+T N  SIPSS IF+ D        DA+PQG  DSIP ID SLL  GTP QR
Sbjct: 1  MASVKSIADTPNLTSIPSSFIFATDDSFDDVTADASPQGAEDSIPIIDLSLLINGTPQQR 60

Query: 66 AKVVDQLGKACQDWGFFM 80
          AKVV++LGKAC+DWGFFM
Sbjct: 61 AKVVNELGKACEDWGFFM 78

[Close]

BLAST of Moc10g03390 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 40.8 bits (94), Expect = 7.9e-04
Identity = 22/71 (30.99%), Postives = 36/71 (50.70%), Query Frame = 0


Query: 8  SIKTIAETLNFASIPSSCIFSGDGGAVDAAPQGVGDSIPTIDFSLLTTGTPDQRAKVVDQ 67
          S++ + +     ++P   + S    A  A   G+ + IP ID SLL + T       +D+
Sbjct: 15 SVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSST--SMDSEIDK 74

Query: 68 LGKACQDWGFF 79
          L  AC++WGFF
Sbjct: 75 LDSACKEWGFF 83

The following BLAST results are available for this feature:

BLAST of Moc10g03390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159287.1	6.8e-50	100.00	uncharacterized protein LOC111025699 [Momordica charantia]	[more]
XP_022149149.1	7.3e-28	82.28	protein DMR6-LIKE OXYGENASE 1-like [Momordica charantia]	[more]
XP_022141027.1	6.8e-26	79.75	protein DMR6-LIKE OXYGENASE 1-like isoform X1 [Momordica charantia] >XP_02215928...	[more]
XP_022141029.1	6.8e-26	79.75	protein DMR6-LIKE OXYGENASE 1-like isoform X2 [Momordica charantia] >XP_02215928...	[more]
XP_023552481.1	2.8e-19	68.24	protein DMR6-LIKE OXYGENASE 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc10g03390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Z244	5.3e-05	37.50	2-oxoglutarate-dependent dioxygenase 19 OS=Oryza sativa subsp. japonica OX=39947...	[more]

BLAST of Moc10g03390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZF0	3.3e-50	100.00	uncharacterized protein LOC111025699 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1D661	3.5e-28	82.28	protein DMR6-LIKE OXYGENASE 1-like OS=Momordica charantia OX=3673 GN=LOC11101763...	[more]
A0A6J1E3G6	3.3e-26	79.75	protein DMR6-LIKE OXYGENASE 1-like isoform X2 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1CIQ0	3.3e-26	79.75	protein DMR6-LIKE OXYGENASE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=...	[more]
A0A5A7UYU4	1.6e-17	64.10	Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]

BLAST of Moc10g03390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25300.1	7.9e-04	30.99	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..99 e-value: 2.5E-10 score: 42.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 45..79 e-value: 8.5E-8 score: 33.0
None	No IPR available	PANTHER	PTHR10209:SF460	FLAVONOL SYNTHASE/FLAVANONE 3-HYDROXYLASE	coord: 9..79
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 9..79
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 20..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g03390.1	Moc10g03390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity