Moc10g03220 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g03220
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUbiquinol oxidase
Locationchr10: 2255009 .. 2256766 (-)
RNA-Seq ExpressionMoc10g03220
SyntenyMoc10g03220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCGGATTGTGATGAGGAATTTCTTTCGGGGTCTGATTAATGGGAGAAGCTTCAGTTCGATTGCCGGTGCCGGCGCCGGTGTTGGTGGTGTGAGGGTTTCTTTGGCGGCGGTGGCCTCGCCGGGGACGAAGTATCAACCGGTGACCGATGGGGGATTCCATTGGCGGAGGATGTTGAGCTCGTCGGCGGCGATGGCGGAGGAACAGGGGAAGGAGAAACTAGAACACAGGATAAAGGATGTAGAGAATCAGGAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGATAATGCTCTGGTTTCTAGCTATTGGGGGATTTACAGGCCGAAGATTACTAGAGAAGACGGCTCCGACTGGCCATGGAATTGCTTTATGGTATGAATTTGGTCCCTCTACTTTTTCCCGTGAACTGTTTGATAAAATAAAACCATGTTCCTTTTTATGGGGTAGTTCGATTTTCTTTGAAATTGCCGGATTTAGTTTTGGAAGCTGCGATTAAAATTTGAAACCTTATGGTCTAGTGTTTTGATTAGATCCACCGCTTCATGTTAAGCATAGAAATCGAGTTTCTGCAACTGTTGGTTTTTATGAGTTGGCTTTTAAAAACTGTTGCTGTAAATGCAGCCATGGGAAACCTACAGAGCGGACGTATCAATTGATCTTGGGAAGCACCACGAGCCGAAAGCTTTTCTGGACAAAGTCGCTTACCGCGTTGTGAAGCTTCTTCGAATTCCAACTGATATCTTCTTCCAGGTGGGTCATTAATTAGCATACATAATTCTGAGGGATTCGTTCAATTTTGAGAACAAACAAGAAAAATAAACGTTTACGTTGTCAAACAAGTCTCTGCTCCTATGGTCTATGGTCTAGAATTCGAGAAATCGTTCATTGTGGCTGGTCACAATATCATCTCTGATTCAGAATCTGTGTGTCCCCAATAACATGGATACGGATTTGAAACATGTTTTGTAATTTCTTCTTCATATATGTAGCTGTATAAAATCACATCAACCACATTGTTGTAGCCCGTCCTGGATTCTAAGTTTTGGATGTTGTGCAGAGGCGGTATGGTTGTCGGGCCGTGATGCTCGAGACGGTGGCAGCCGTCCCGGGGATGGTCGGAGGGATGCTGCTGCATCTGAAATCTCTGAGAAAGTTTGAGCACAGTGGAGGGTGGATCAAGGCCTTGCTTGAAGAAGCAGAGAATGAGAGGATGCATCTAATGACCATGATTGAGCTTGTGCAGCCCAAGTGGTACGAGCGGCTGCTGGTGATCACAGTGCAGGGCGTCTTCTTCAATGCCTTCTTTGTGCTCTATCTGATGTCCCCAAAATTGGCTCATAGAATTGTTGGCTATTTGGAAGAGGAAGCCATCCATTCCTACACCGAGTACTTGAAGGATATTGAGAACGGTAAGATCGAGAACGTGCCCGCTCCCGCCATTGCCATCGACTACTGGAGGCTGCCTAAGGATGCAAGGTTGAAGGATGTCATCATGGTTATTCGGGCCGATGAGGCGCACCACCGCGATGTCAACCATTTTGCTTCTGTGAGTAACTTGATTCTTTTGTGCTTGTAACTCTAGAAATGCTGTGTGGGAATCTACAAAAATTAGAATGCAACTGTTGCAGTGAACATATTTTGAACATGTTTCGCTGAAATGGCCTGGTTTTCTGTTGTGTGTAGGACATCCATTTCCAAGGAAAGGAACTGAAAGAATCAGCAGCCCCTCTTGGATATCACTAA

mRNA sequence

ATGAATCGGATTGTGATGAGGAATTTCTTTCGGGGTCTGATTAATGGGAGAAGCTTCAGTTCGATTGCCGGTGCCGGCGCCGGTGTTGGTGGTGTGAGGGTTTCTTTGGCGGCGGTGGCCTCGCCGGGGACGAAGTATCAACCGGTGACCGATGGGGGATTCCATTGGCGGAGGATGTTGAGCTCGTCGGCGGCGATGGCGGAGGAACAGGGGAAGGAGAAACTAGAACACAGGATAAAGGATGTAGAGAATCAGGAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGATAATGCTCTGGTTTCTAGCTATTGGGGGATTTACAGGCCGAAGATTACTAGAGAAGACGGCTCCGACTGGCCATGGAATTGCTTTATGCCATGGGAAACCTACAGAGCGGACGTATCAATTGATCTTGGGAAGCACCACGAGCCGAAAGCTTTTCTGGACAAAGTCGCTTACCGCGTTGTGAAGCTTCTTCGAATTCCAACTGATATCTTCTTCCAGAGGCGGTATGGTTGTCGGGCCGTGATGCTCGAGACGGTGGCAGCCGTCCCGGGGATGGTCGGAGGGATGCTGCTGCATCTGAAATCTCTGAGAAAGTTTGAGCACAGTGGAGGGTGGATCAAGGCCTTGCTTGAAGAAGCAGAGAATGAGAGGATGCATCTAATGACCATGATTGAGCTTGTGCAGCCCAAGTGGTACGAGCGGCTGCTGGTGATCACAGTGCAGGGCGTCTTCTTCAATGCCTTCTTTGTGCTCTATCTGATGTCCCCAAAATTGGCTCATAGAATTGTTGGCTATTTGGAAGAGGAAGCCATCCATTCCTACACCGAGTACTTGAAGGATATTGAGAACGGTAAGATCGAGAACGTGCCCGCTCCCGCCATTGCCATCGACTACTGGAGGCTGCCTAAGGATGCAAGGTTGAAGGATGTCATCATGGTTATTCGGGCCGATGAGGCGCACCACCGCGATGTCAACCATTTTGCTTCTGACATCCATTTCCAAGGAAAGGAACTGAAAGAATCAGCAGCCCCTCTTGGATATCACTAA

Coding sequence (CDS)

ATGAATCGGATTGTGATGAGGAATTTCTTTCGGGGTCTGATTAATGGGAGAAGCTTCAGTTCGATTGCCGGTGCCGGCGCCGGTGTTGGTGGTGTGAGGGTTTCTTTGGCGGCGGTGGCCTCGCCGGGGACGAAGTATCAACCGGTGACCGATGGGGGATTCCATTGGCGGAGGATGTTGAGCTCGTCGGCGGCGATGGCGGAGGAACAGGGGAAGGAGAAACTAGAACACAGGATAAAGGATGTAGAGAATCAGGAGCAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGATAATGCTCTGGTTTCTAGCTATTGGGGGATTTACAGGCCGAAGATTACTAGAGAAGACGGCTCCGACTGGCCATGGAATTGCTTTATGCCATGGGAAACCTACAGAGCGGACGTATCAATTGATCTTGGGAAGCACCACGAGCCGAAAGCTTTTCTGGACAAAGTCGCTTACCGCGTTGTGAAGCTTCTTCGAATTCCAACTGATATCTTCTTCCAGAGGCGGTATGGTTGTCGGGCCGTGATGCTCGAGACGGTGGCAGCCGTCCCGGGGATGGTCGGAGGGATGCTGCTGCATCTGAAATCTCTGAGAAAGTTTGAGCACAGTGGAGGGTGGATCAAGGCCTTGCTTGAAGAAGCAGAGAATGAGAGGATGCATCTAATGACCATGATTGAGCTTGTGCAGCCCAAGTGGTACGAGCGGCTGCTGGTGATCACAGTGCAGGGCGTCTTCTTCAATGCCTTCTTTGTGCTCTATCTGATGTCCCCAAAATTGGCTCATAGAATTGTTGGCTATTTGGAAGAGGAAGCCATCCATTCCTACACCGAGTACTTGAAGGATATTGAGAACGGTAAGATCGAGAACGTGCCCGCTCCCGCCATTGCCATCGACTACTGGAGGCTGCCTAAGGATGCAAGGTTGAAGGATGTCATCATGGTTATTCGGGCCGATGAGGCGCACCACCGCGATGTCAACCATTTTGCTTCTGACATCCATTTCCAAGGAAAGGAACTGAAAGAATCAGCAGCCCCTCTTGGATATCACTAA

Protein sequence

MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRMLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH
Homology
BLAST of Moc10g03220 vs. NCBI nr
Match: XP_022141101.1 (ubiquinol oxidase, mitochondrial [Momordica charantia])

HSP 1 Score: 708.8 bits (1828), Expect = 2.4e-200
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML
Sbjct: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG
Sbjct: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV
Sbjct: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER
Sbjct: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI
Sbjct: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH
Sbjct: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 354

BLAST of Moc10g03220 vs. NCBI nr
Match: AEN99850.1 (mitochondrial AOX2 [Citrullus lanatus])

HSP 1 Score: 622.1 bits (1603), Expect = 3.0e-174
Identity = 309/356 (86.80%), Postives = 328/356 (92.13%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAG--AGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRR 60
           MNRIV+R+F RGL+N RSF S+AG   G   GG++V+ AAVASPGT+YQP+ DGGF WRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  MLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITRE 120
           +LSSS A+AEEQGK KLE  IKD+ENQE       +KEKE +NALVSSYWGIYRPKITRE
Sbjct: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQE-------KKEKETENALVSSYWGIYRPKITRE 120

Query: 121 DGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180
           DGS+WPWNCFMPWETYRAD+SIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCR
Sbjct: 121 DGSEWPWNCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180

Query: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWY 240
           AVMLETVAAVPGMVGGMLLHLKSLRKF+HSG WIKALLEEAENERMHLMTMIELVQPKWY
Sbjct: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFQHSGVWIKALLEEAENERMHLMTMIELVQPKWY 240

Query: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAP 300
           ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDI+ GKIENVPAP
Sbjct: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAP 300

Query: 301 AIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIAIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESAAPLGYH
Sbjct: 301 AIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 349

BLAST of Moc10g03220 vs. NCBI nr
Match: XP_022985158.1 (ubiquinol oxidase, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 621.3 bits (1601), Expect = 5.1e-174
Identity = 309/354 (87.29%), Postives = 328/354 (92.66%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           MNRIV+R+F RGL+N RSF SIAG G+G GG++V+ AA ASP T+YQP+ DGGF WRR++
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSIAG-GSG-GGMKVTFAAAASPSTRYQPLADGGFQWRRLM 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           SSS A+AEEQGK KLE   KDVENQ        EKEKEKDNALVSSYWGIYRPKITREDG
Sbjct: 61  SSSTAVAEEQGKVKLEQGNKDVENQ--------EKEKEKDNALVSSYWGIYRPKITREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           S+WPWNCFMPWETYRADVSIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCRAV
Sbjct: 121 SEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLETVAAVPGMVGGMLLHLKSLR+F+HSGGWIKALLEEAENERMHLMTMIELVQPKWYER
Sbjct: 181 MLETVAAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           +LVITVQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDI+ GKIENVPAPAI
Sbjct: 241 MLVITVQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESAAPLGYH
Sbjct: 301 AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Moc10g03220 vs. NCBI nr
Match: ADD84880.1 (mitochondrial AOX2 [Citrullus lanatus])

HSP 1 Score: 620.9 bits (1600), Expect = 6.6e-174
Identity = 307/356 (86.24%), Postives = 326/356 (91.57%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAG--AGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRR 60
           MNRI +R+F RGL+N RSF S+AG   G   GG++V+ AAVASPGT+YQP+ DGGF WRR
Sbjct: 1   MNRIAIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  MLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITRE 120
           +LSSS A+ EEQGK KLE  IKD+ENQE       +KEKE +NALVSSYWGIYRPKITRE
Sbjct: 61  LLSSSTAVTEEQGKVKLEQGIKDIENQE-------KKEKETENALVSSYWGIYRPKITRE 120

Query: 121 DGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180
           DGS+WPWNCFMPWETYRAD+SIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCR
Sbjct: 121 DGSEWPWNCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180

Query: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWY 240
           AVMLETVAAVPGMVGGMLLHLKSLRKF+HSGGWIKALLEEAENERMHLMTMIELVQPKWY
Sbjct: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWY 240

Query: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAP 300
           ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDI+ GKIENVPAP
Sbjct: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAP 300

Query: 301 AIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIAIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESA PLGYH
Sbjct: 301 AIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAVPLGYH 349

BLAST of Moc10g03220 vs. NCBI nr
Match: KAG6576858.1 (Ubiquinol oxidase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.0 bits (1595), Expect = 2.5e-173
Identity = 308/354 (87.01%), Postives = 329/354 (92.94%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           MNRIV+R+F RGL+N RS  SIAG G+G GG++V+ AA ASP T+YQP+ DGGF WRR++
Sbjct: 1   MNRIVIRSFVRGLVNSRSSGSIAG-GSG-GGMKVTFAAAASPSTRYQPLADGGFQWRRLM 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           SSS+A+AEEQGK KLE   KDVENQ        EKEKEKDNALVSSYWGIYRPKITREDG
Sbjct: 61  SSSSAVAEEQGKVKLEQGNKDVENQ--------EKEKEKDNALVSSYWGIYRPKITREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           S+WPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV
Sbjct: 121 SEWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLETVAAVPGMVGGMLLHLKSLR+F+HSGGWIKALLEEAENERMHLMTMIELVQPKWYER
Sbjct: 181 MLETVAAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           +LVITVQGVFFNAFFVLY+MSPKLAHR+VGYLEEEAIHSYTEYLKDI+ GKIENVPAPAI
Sbjct: 241 MLVITVQGVFFNAFFVLYVMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESAAPLGYH
Sbjct: 301 AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Moc10g03220 vs. ExPASy Swiss-Prot
Match: Q41266 (Ubiquinol oxidase 2, mitochondrial OS=Glycine max OX=3847 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 474.9 bits (1221), Expect = 7.7e-133
Identity = 241/345 (69.86%), Postives = 276/345 (80.00%), Query Frame = 0

Query: 11  RGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGG-FHWRRMLSSSAAMAEE 70
           R L+NGR+ +     G  +G   +   A A          +G  F+W+R + S A     
Sbjct: 12  RALLNGRNQN-----GNRLGSAALMPYAAAETRLLCAGGANGWFFYWKRTMVSPA----- 71

Query: 71  QGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDGSDWPWNCFM 130
                        E +  EKEKEKEK K + + + SSYWGI RPK+ REDG++WPWNCFM
Sbjct: 72  -------------EAKVPEKEKEKEKAKAEKSVVESSYWGISRPKVVREDGTEWPWNCFM 131

Query: 131 PWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVP 190
           PWE+YR++VSIDL KHH PK  LDKVAYR VKLLRIPTD+FF+RRYGCRA+MLETVAAVP
Sbjct: 132 PWESYRSNVSIDLTKHHVPKNVLDKVAYRTVKLLRIPTDLFFKRRYGCRAMMLETVAAVP 191

Query: 191 GMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGV 250
           GMVGGMLLHL+SLRKF+ SGGWIKALLEEAENERMHLMTM+ELV+PKWYERLLV+ VQGV
Sbjct: 192 GMVGGMLLHLRSLRKFQQSGGWIKALLEEAENERMHLMTMVELVKPKWYERLLVLAVQGV 251

Query: 251 FFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAIAIDYWRLPK 310
           FFNAFFVLY++SPK+AHRIVGYLEEEAIHSYTEYLKD+E+G IENVPAPAIAIDYWRLPK
Sbjct: 252 FFNAFFVLYILSPKVAHRIVGYLEEEAIHSYTEYLKDLESGAIENVPAPAIAIDYWRLPK 311

Query: 311 DARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           DARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+E+ AP+GYH
Sbjct: 312 DARLKDVITVIRADEAHHRDVNHFASDIHFQGKELREAPAPIGYH 333

BLAST of Moc10g03220 vs. ExPASy Swiss-Prot
Match: Q40294 (Ubiquinol oxidase, mitochondrial OS=Mangifera indica OX=29780 GN=AOMI 1 PE=1 SV=2)

HSP 1 Score: 471.1 bits (1211), Expect = 1.1e-131
Identity = 227/299 (75.92%), Postives = 257/299 (85.95%), Query Frame = 0

Query: 56  WRRMLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKI 115
           WRRML S+A  AE Q                      KE+++EK +A+VS+YWGI RPKI
Sbjct: 42  WRRML-SNAGGAEAQ---------------------VKEQKEEKKDAMVSNYWGISRPKI 101

Query: 116 TREDGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRY 175
           TREDGS+WPWNCFMPWETYR+D+SIDL KHH P+ F+DK AYR VK+LR+PTDIFFQRRY
Sbjct: 102 TREDGSEWPWNCFMPWETYRSDLSIDLKKHHVPRTFMDKFAYRTVKILRVPTDIFFQRRY 161

Query: 176 GCRAVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQP 235
           GCRA+MLETVAAVPGMVGGMLLHLKSLRK E SGGWIKALLEEAENERMHLMTM+ELVQP
Sbjct: 162 GCRAMMLETVAAVPGMVGGMLLHLKSLRKLEQSGGWIKALLEEAENERMHLMTMVELVQP 221

Query: 236 KWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENV 295
           KWYERLLV+ VQGVFFN+FFVLY++SPKLAHRIVGYLEEEAIHSYTEYLKDI++G I+N+
Sbjct: 222 KWYERLLVLAVQGVFFNSFFVLYVLSPKLAHRIVGYLEEEAIHSYTEYLKDIDSGAIKNI 281

Query: 296 PAPAIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           PAPAIAIDYWRLPKDA LKDVI V+RADEAHHRDVNHFASD+  QGKEL+++ AP+GYH
Sbjct: 282 PAPAIAIDYWRLPKDATLKDVITVVRADEAHHRDVNHFASDVQVQGKELRDAPAPVGYH 318

BLAST of Moc10g03220 vs. ExPASy Swiss-Prot
Match: O22049 (Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 SV=2)

HSP 1 Score: 446.8 bits (1148), Expect = 2.3e-124
Identity = 223/305 (73.11%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 52  GGFHWRRMLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGI- 111
           G F+ R M  SSA+  E                ++ E    K+ +    +  V SYWGI 
Sbjct: 65  GNFNLRWMGMSSASAME----------------KKDENLTVKKGQNGGGSVAVPSYWGIE 124

Query: 112 -YRPKITREDGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDI 171
             + KITR+DGSDWPWNCFMPWETY+A++SIDL KHH PK   DKVAYR+VKLLRIPTDI
Sbjct: 125 TAKMKITRKDGSDWPWNCFMPWETYQANLSIDLKKHHVPKNIADKVAYRIVKLLRIPTDI 184

Query: 172 FFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTM 231
           FFQRRYGCRA+MLETVAAVPGMVGGMLLHLKS+RKFEHSGGWIKALLEEAENERMHLMTM
Sbjct: 185 FFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLMTM 244

Query: 232 IELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIEN 291
           +ELV+PKWYERLLV+ VQG+FFN+FFV Y++SP+LAHR+VGYLEEEAIHSYTE+LKDI+N
Sbjct: 245 MELVKPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKDIDN 304

Query: 292 GKIENVPAPAIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAA 351
           GKIENV APAIAIDYWRLPKDA LKDV+ VIRADEAHHRDVNHFASDI  QGKEL+E+AA
Sbjct: 305 GKIENVAAPAIAIDYWRLPKDATLKDVVTVIRADEAHHRDVNHFASDIRNQGKELREAAA 353

Query: 352 PLGYH 355
           P+GYH
Sbjct: 365 PIGYH 353

BLAST of Moc10g03220 vs. ExPASy Swiss-Prot
Match: O03376 (Alternative oxidase 3, mitochondrial OS=Glycine max OX=3847 GN=AOX3 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-121
Identity = 217/354 (61.30%), Postives = 264/354 (74.58%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           M  +++R+  R L+ G   S            ++S AA+     ++     G FH RRM 
Sbjct: 1   MKNVLVRSAARALLGGGGRSYYR---------QLSTAAIVEQRHQHGGGAFGSFHLRRMS 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           +                     E ++Q  E++K +     NA+V+SYWGI RPK+ REDG
Sbjct: 61  TLP-------------------EVKDQHSEEKKNEVNGTSNAVVTSYWGITRPKVRREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           ++WPWNCFMPW++Y +DVSID+ KHH PK+  DKVA+R VK LR+ +DI+F+ RYGC A+
Sbjct: 121 TEWPWNCFMPWDSYHSDVSIDVTKHHTPKSLTDKVAFRAVKFLRVLSDIYFKERYGCHAM 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLET+AAVPGMVGGMLLHLKSLRKF+HSGGWIKALLEEAENERMHLMTM+ELV+P W+ER
Sbjct: 181 MLETIAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMVELVKPSWHER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           LL+ T QGVFFNAFFV YL+SPK AHR VGYLEEEA+ SYT++L  IE+GK+ENVPAPAI
Sbjct: 241 LLIFTAQGVFFNAFFVFYLLSPKAAHRFVGYLEEEAVISYTQHLNAIESGKVENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDA LKDV+ VIRADEAHHRDVNHFASDIH QGKELKE+ AP+GYH
Sbjct: 301 AIDYWRLPKDATLKDVVTVIRADEAHHRDVNHFASDIHHQGKELKEAPAPIGYH 326

BLAST of Moc10g03220 vs. ExPASy Swiss-Prot
Match: O82807 (Ubiquinol oxidase 1a, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=AOX1A PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.4e-118
Identity = 216/335 (64.48%), Postives = 254/335 (75.82%), Query Frame = 0

Query: 29  VGGVRVSLAAVASP----GTKYQPVTDGGFH----WRRMLSSSAAMAEEQGKEKLEHRIK 88
           VGGVR+  A+  SP        +P   GG      W   L S++++A  +   K E    
Sbjct: 14  VGGVRLFTASATSPAAAAAAAARPFLAGGEAVPGVWGLRLMSTSSVASTEAAAKAE---- 73

Query: 89  DVENQEQEKEKEKEKEKEKDNALVSSYWGIYR-PKITREDGSDWPWNCFMPWETYRADVS 148
                     K+ + EKE    +V+SYWGI +  K+ REDG++W W+CF PWETY AD S
Sbjct: 74  ---------AKKADAEKE---VVVNSYWGIEQSKKLVREDGTEWKWSCFRPWETYTADTS 133

Query: 149 IDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHL 208
           IDL KHH PK  LDK+AY  VK LR PTDIFFQRRYGCRA+MLETVAAVPGMVGGMLLHL
Sbjct: 134 IDLTKHHVPKTLLDKIAYWTVKSLRFPTDIFFQRRYGCRAMMLETVAAVPGMVGGMLLHL 193

Query: 209 KSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYL 268
           +SLR+FE SGGWI+ LLEEAENERMHLMT +E+  PKWYER LVITVQGVFFNA+F+ YL
Sbjct: 194 RSLRRFEQSGGWIRTLLEEAENERMHLMTFMEVANPKWYERALVITVQGVFFNAYFLGYL 253

Query: 269 MSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAIAIDYWRLPKDARLKDVIMV 328
           +SPK AHR+VGYLEEEAIHSYTE+LKD+E GKI+NVPAPAIAIDYWRLP +A LKDV+ V
Sbjct: 254 LSPKFAHRVVGYLEEEAIHSYTEFLKDLEAGKIDNVPAPAIAIDYWRLPANATLKDVVTV 313

Query: 329 IRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           +RADEAHHRDVNHFASDIH+QG ELK++ AP+GYH
Sbjct: 314 VRADEAHHRDVNHFASDIHYQGMELKQTPAPIGYH 332

BLAST of Moc10g03220 vs. ExPASy TrEMBL
Match: A0A6J1CI07 (Ubiquinol oxidase OS=Momordica charantia OX=3673 GN=LOC111011576 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.2e-200
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML
Sbjct: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG
Sbjct: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV
Sbjct: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER
Sbjct: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI
Sbjct: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH
Sbjct: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 354

BLAST of Moc10g03220 vs. ExPASy TrEMBL
Match: G3G3W9 (Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.4e-174
Identity = 309/356 (86.80%), Postives = 328/356 (92.13%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAG--AGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRR 60
           MNRIV+R+F RGL+N RSF S+AG   G   GG++V+ AAVASPGT+YQP+ DGGF WRR
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  MLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITRE 120
           +LSSS A+AEEQGK KLE  IKD+ENQE       +KEKE +NALVSSYWGIYRPKITRE
Sbjct: 61  LLSSSTAVAEEQGKVKLEQGIKDIENQE-------KKEKETENALVSSYWGIYRPKITRE 120

Query: 121 DGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180
           DGS+WPWNCFMPWETYRAD+SIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCR
Sbjct: 121 DGSEWPWNCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180

Query: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWY 240
           AVMLETVAAVPGMVGGMLLHLKSLRKF+HSG WIKALLEEAENERMHLMTMIELVQPKWY
Sbjct: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFQHSGVWIKALLEEAENERMHLMTMIELVQPKWY 240

Query: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAP 300
           ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDI+ GKIENVPAP
Sbjct: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAP 300

Query: 301 AIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIAIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESAAPLGYH
Sbjct: 301 AIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 349

BLAST of Moc10g03220 vs. ExPASy TrEMBL
Match: A0A6J1JCS2 (Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111483245 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.5e-174
Identity = 309/354 (87.29%), Postives = 328/354 (92.66%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           MNRIV+R+F RGL+N RSF SIAG G+G GG++V+ AA ASP T+YQP+ DGGF WRR++
Sbjct: 1   MNRIVIRSFVRGLVNSRSFGSIAG-GSG-GGMKVTFAAAASPSTRYQPLADGGFQWRRLM 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           SSS A+AEEQGK KLE   KDVENQ        EKEKEKDNALVSSYWGIYRPKITREDG
Sbjct: 61  SSSTAVAEEQGKVKLEQGNKDVENQ--------EKEKEKDNALVSSYWGIYRPKITREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           S+WPWNCFMPWETYRADVSIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCRAV
Sbjct: 121 SEWPWNCFMPWETYRADVSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLETVAAVPGMVGGMLLHLKSLR+F+HSGGWIKALLEEAENERMHLMTMIELVQPKWYER
Sbjct: 181 MLETVAAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           +LVITVQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDI+ GKIENVPAPAI
Sbjct: 241 MLVITVQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESAAPLGYH
Sbjct: 301 AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Moc10g03220 vs. ExPASy TrEMBL
Match: D4P4J8 (Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.2e-174
Identity = 307/356 (86.24%), Postives = 326/356 (91.57%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAG--AGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRR 60
           MNRI +R+F RGL+N RSF S+AG   G   GG++V+ AAVASPGT+YQP+ DGGF WRR
Sbjct: 1   MNRIAIRSFVRGLVNSRSFGSVAGVAGGGSGGGMKVTFAAVASPGTRYQPMVDGGFQWRR 60

Query: 61  MLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITRE 120
           +LSSS A+ EEQGK KLE  IKD+ENQE       +KEKE +NALVSSYWGIYRPKITRE
Sbjct: 61  LLSSSTAVTEEQGKVKLEQGIKDIENQE-------KKEKETENALVSSYWGIYRPKITRE 120

Query: 121 DGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180
           DGS+WPWNCFMPWETYRAD+SIDLGKHHEPK FLDKVAYRVVKLLRIPTDIFFQRRYGCR
Sbjct: 121 DGSEWPWNCFMPWETYRADLSIDLGKHHEPKTFLDKVAYRVVKLLRIPTDIFFQRRYGCR 180

Query: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWY 240
           AVMLETVAAVPGMVGGMLLHLKSLRKF+HSGGWIKALLEEAENERMHLMTMIELVQPKWY
Sbjct: 181 AVMLETVAAVPGMVGGMLLHLKSLRKFQHSGGWIKALLEEAENERMHLMTMIELVQPKWY 240

Query: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAP 300
           ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDI+ GKIENVPAP
Sbjct: 241 ERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIDEGKIENVPAP 300

Query: 301 AIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIAIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESA PLGYH
Sbjct: 301 AIAIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAVPLGYH 349

BLAST of Moc10g03220 vs. ExPASy TrEMBL
Match: A0A6J1E6M0 (Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111430458 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.6e-173
Identity = 306/354 (86.44%), Postives = 326/354 (92.09%), Query Frame = 0

Query: 1   MNRIVMRNFFRGLINGRSFSSIAGAGAGVGGVRVSLAAVASPGTKYQPVTDGGFHWRRML 60
           MNR+V+R+  RGL+N RSF SIAG     GG++V+ AA ASP T+YQP+ DGGF WRR++
Sbjct: 1   MNRLVIRSVVRGLVNSRSFGSIAGDSG--GGMKVTFAAAASPSTRYQPLADGGFQWRRLM 60

Query: 61  SSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDG 120
           SSS+A+AEEQGK KLE   KDVENQ        EKEKEKDNALVSSYWGIYRPKITREDG
Sbjct: 61  SSSSAVAEEQGKVKLEQGNKDVENQ--------EKEKEKDNALVSSYWGIYRPKITREDG 120

Query: 121 SDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180
           S+WPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV
Sbjct: 121 SEWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAV 180

Query: 181 MLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240
           MLETVAAVPGMVGGMLLHLKSLR+F+HSGGWIKALLEEAENERMHLMTMIELVQPKWYER
Sbjct: 181 MLETVAAVPGMVGGMLLHLKSLRRFQHSGGWIKALLEEAENERMHLMTMIELVQPKWYER 240

Query: 241 LLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAI 300
           +LVITVQGVFFNAFFVLYLMSPKLAHR+VGYLEEEAIHSYTEYLKDI+ GKIENVPAPAI
Sbjct: 241 MLVITVQGVFFNAFFVLYLMSPKLAHRVVGYLEEEAIHSYTEYLKDIDEGKIENVPAPAI 300

Query: 301 AIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           AIDYWRLPKDARLKDVI VIRADEAHHRDVNHFASDIHFQGKEL+ESAAPLGYH
Sbjct: 301 AIDYWRLPKDARLKDVITVIRADEAHHRDVNHFASDIHFQGKELRESAAPLGYH 344

BLAST of Moc10g03220 vs. TAIR 10
Match: AT5G64210.1 (alternative oxidase 2 )

HSP 1 Score: 446.8 bits (1148), Expect = 1.6e-125
Identity = 223/305 (73.11%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 52  GGFHWRRMLSSSAAMAEEQGKEKLEHRIKDVENQEQEKEKEKEKEKEKDNALVSSYWGI- 111
           G F+ R M  SSA+  E                ++ E    K+ +    +  V SYWGI 
Sbjct: 65  GNFNLRWMGMSSASAME----------------KKDENLTVKKGQNGGGSVAVPSYWGIE 124

Query: 112 -YRPKITREDGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDI 171
             + KITR+DGSDWPWNCFMPWETY+A++SIDL KHH PK   DKVAYR+VKLLRIPTDI
Sbjct: 125 TAKMKITRKDGSDWPWNCFMPWETYQANLSIDLKKHHVPKNIADKVAYRIVKLLRIPTDI 184

Query: 172 FFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTM 231
           FFQRRYGCRA+MLETVAAVPGMVGGMLLHLKS+RKFEHSGGWIKALLEEAENERMHLMTM
Sbjct: 185 FFQRRYGCRAMMLETVAAVPGMVGGMLLHLKSIRKFEHSGGWIKALLEEAENERMHLMTM 244

Query: 232 IELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIEN 291
           +ELV+PKWYERLLV+ VQG+FFN+FFV Y++SP+LAHR+VGYLEEEAIHSYTE+LKDI+N
Sbjct: 245 MELVKPKWYERLLVMLVQGIFFNSFFVCYVISPRLAHRVVGYLEEEAIHSYTEFLKDIDN 304

Query: 292 GKIENVPAPAIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAA 351
           GKIENV APAIAIDYWRLPKDA LKDV+ VIRADEAHHRDVNHFASDI  QGKEL+E+AA
Sbjct: 305 GKIENVAAPAIAIDYWRLPKDATLKDVVTVIRADEAHHRDVNHFASDIRNQGKELREAAA 353

Query: 352 PLGYH 355
           P+GYH
Sbjct: 365 PIGYH 353

BLAST of Moc10g03220 vs. TAIR 10
Match: AT3G22370.1 (alternative oxidase 1A )

HSP 1 Score: 422.2 bits (1084), Expect = 4.2e-118
Identity = 201/285 (70.53%), Postives = 238/285 (83.51%), Query Frame = 0

Query: 73  EKLEHRIKDVENQEQEKEK---EKEKEKEKDNALVSSYWGIYRPKITREDGSDWPWNCFM 132
           EK   + +D   ++ E E    +      K +  ++SYWG+   KIT+EDGS+W WNCF 
Sbjct: 70  EKTPMKEEDANQKKTENESTGGDAAGGNNKGDKGIASYWGVEPNKITKEDGSEWKWNCFR 129

Query: 133 PWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVP 192
           PWETY+AD++IDL KHH P  FLD++AY  VK LR PTD+FFQRRYGCRA+MLETVAAVP
Sbjct: 130 PWETYKADITIDLKKHHVPTTFLDRIAYWTVKSLRWPTDLFFQRRYGCRAMMLETVAAVP 189

Query: 193 GMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGV 252
           GMVGGMLLH KSLR+FE SGGWIKALLEEAENERMHLMT +E+ +PKWYER LVITVQGV
Sbjct: 190 GMVGGMLLHCKSLRRFEQSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVITVQGV 249

Query: 253 FFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAIAIDYWRLPK 312
           FFNA+F+ YL+SPK AHR+VGYLEEEAIHSYTE+LK+++ G IENVPAPAIAIDYWRLP 
Sbjct: 250 FFNAYFLGYLISPKFAHRMVGYLEEEAIHSYTEFLKELDKGNIENVPAPAIAIDYWRLPA 309

Query: 313 DARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           DA L+DV+MV+RADEAHHRDVNHFASDIH+QG+ELKE+ AP+GYH
Sbjct: 310 DATLRDVVMVVRADEAHHRDVNHFASDIHYQGRELKEAPAPIGYH 354

BLAST of Moc10g03220 vs. TAIR 10
Match: AT3G27620.1 (alternative oxidase 1C )

HSP 1 Score: 413.3 bits (1061), Expect = 2.0e-115
Identity = 194/288 (67.36%), Postives = 235/288 (81.60%), Query Frame = 0

Query: 79  IKDVENQEQEKEKEKEKEK------------EKDNALVSSYWGIYRPKITREDGSDWPWN 138
           ++D      EK+K  E+E+             K   L+ SYWG+   KIT+EDG++W W+
Sbjct: 42  LRDFSKMTFEKKKTSEEEEGSGDGVKVNDQGNKGEQLIVSYWGVKPMKITKEDGTEWKWS 101

Query: 139 CFMPWETYRADVSIDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVA 198
           CF PWETY+AD++IDL KHH P    DK+AY +VK LR PTD+FFQRRYGCRA+MLETVA
Sbjct: 102 CFRPWETYKADLTIDLKKHHVPSTLPDKIAYWMVKSLRWPTDLFFQRRYGCRAIMLETVA 161

Query: 199 AVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITV 258
           AVPGMVGGML+H KSLR+FE SGGWIKALLEEAENERMHLMT +E+ +PKWYER LVI+V
Sbjct: 162 AVPGMVGGMLMHFKSLRRFEQSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVISV 221

Query: 259 QGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAIAIDYWR 318
           QGVFFNA+ + Y++SPK AHR+VGYLEEEAIHSYTE+LK+++NG IENVPAPAIA+DYWR
Sbjct: 222 QGVFFNAYLIGYIISPKFAHRMVGYLEEEAIHSYTEFLKELDNGNIENVPAPAIAVDYWR 281

Query: 319 LPKDARLKDVIMVIRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           L  DA L+DV+MV+RADEAHHRDVNH+ASDIH+QG ELKE+ AP+GYH
Sbjct: 282 LEADATLRDVVMVVRADEAHHRDVNHYASDIHYQGHELKEAPAPIGYH 329

BLAST of Moc10g03220 vs. TAIR 10
Match: AT1G32350.1 (alternative oxidase 1D )

HSP 1 Score: 409.1 bits (1050), Expect = 3.7e-114
Identity = 186/255 (72.94%), Postives = 219/255 (85.88%), Query Frame = 0

Query: 100 DNALVSSYWGIYRPKITREDGSDWPWNCFMPWETYRADVSIDLGKHHEPKAFLDKVAYRV 159
           D  ++S+YWGI   KIT+ DGS W WNCF PW++Y+ DVSID+ KHH+P  F DK AY  
Sbjct: 64  DGKVISTYWGIPPTKITKPDGSAWKWNCFQPWDSYKPDVSIDVTKHHKPSNFTDKFAYWT 123

Query: 160 VKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHLKSLRKFEHSGGWIKALLEEA 219
           V+ L+IP  +FFQR++ C A++LETVAAVPGMVGGMLLHLKSLR+FEHSGGWIKALLEEA
Sbjct: 124 VQTLKIPVQLFFQRKHMCHAMLLETVAAVPGMVGGMLLHLKSLRRFEHSGGWIKALLEEA 183

Query: 220 ENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYLMSPKLAHRIVGYLEEEAIHS 279
           ENERMHLMT IEL QPKWYER +V TVQGVFFNA+F+ Y++SPKLAHRI GYLEEEA++S
Sbjct: 184 ENERMHLMTFIELSQPKWYERAIVFTVQGVFFNAYFLAYVISPKLAHRITGYLEEEAVNS 243

Query: 280 YTEYLKDIENGKIENVPAPAIAIDYWRLPKDARLKDVIMVIRADEAHHRDVNHFASDIHF 339
           YTE+LKDI+ GK EN PAPAIAIDYWRLPKDA L+DV+ VIRADEAHHRD+NH+ASDI F
Sbjct: 244 YTEFLKDIDAGKFENSPAPAIAIDYWRLPKDATLRDVVYVIRADEAHHRDINHYASDIQF 303

Query: 340 QGKELKESAAPLGYH 355
           +G ELKE+ AP+GYH
Sbjct: 304 KGHELKEAPAPIGYH 318

BLAST of Moc10g03220 vs. TAIR 10
Match: AT3G22360.1 (alternative oxidase 1B )

HSP 1 Score: 409.1 bits (1050), Expect = 3.7e-114
Identity = 191/275 (69.45%), Postives = 229/275 (83.27%), Query Frame = 0

Query: 80  KDVENQEQEKEKEKEKEKEKDNALVSSYWGIYRPKITREDGSDWPWNCFMPWETYRADVS 139
           K    +E+     K  +  K   L+ SYWG+   KIT+EDG++W W+CF PWETY++D++
Sbjct: 51  KKKTTEEKGSSGGKADQGNKGEQLIVSYWGVKPMKITKEDGTEWKWSCFRPWETYKSDLT 110

Query: 140 IDLGKHHEPKAFLDKVAYRVVKLLRIPTDIFFQRRYGCRAVMLETVAAVPGMVGGMLLHL 199
           IDL KHH P    DK+AY  VK LR PTD+FFQRRYGCRA+MLETVAAVPGMVGGML+H 
Sbjct: 111 IDLKKHHVPSTLPDKLAYWTVKSLRWPTDLFFQRRYGCRAMMLETVAAVPGMVGGMLVHC 170

Query: 200 KSLRKFEHSGGWIKALLEEAENERMHLMTMIELVQPKWYERLLVITVQGVFFNAFFVLYL 259
           KSLR+FE SGGWIKALLEEAENERMHLMT +E+ +P WYER LVI VQG+FFNA+F+ YL
Sbjct: 171 KSLRRFEQSGGWIKALLEEAENERMHLMTFMEVAKPNWYERALVIAVQGIFFNAYFLGYL 230

Query: 260 MSPKLAHRIVGYLEEEAIHSYTEYLKDIENGKIENVPAPAIAIDYWRLPKDARLKDVIMV 319
           +SPK AHR+VGYLEEEAIHSYTE+LK+++NG IENVPAPAIAIDYWRL  DA L+DV+MV
Sbjct: 231 ISPKFAHRMVGYLEEEAIHSYTEFLKELDNGNIENVPAPAIAIDYWRLEADATLRDVVMV 290

Query: 320 IRADEAHHRDVNHFASDIHFQGKELKESAAPLGYH 355
           +RADEAHHRDVNH+ASDIH+QG+ELKE+ AP+GYH
Sbjct: 291 VRADEAHHRDVNHYASDIHYQGRELKEAPAPIGYH 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141101.12.4e-200100.00ubiquinol oxidase, mitochondrial [Momordica charantia][more]
AEN99850.13.0e-17486.80mitochondrial AOX2 [Citrullus lanatus][more]
XP_022985158.15.1e-17487.29ubiquinol oxidase, mitochondrial-like [Cucurbita maxima][more]
ADD84880.16.6e-17486.24mitochondrial AOX2 [Citrullus lanatus][more]
KAG6576858.12.5e-17387.01Ubiquinol oxidase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
Q412667.7e-13369.86Ubiquinol oxidase 2, mitochondrial OS=Glycine max OX=3847 GN=AOX2 PE=1 SV=2[more]
Q402941.1e-13175.92Ubiquinol oxidase, mitochondrial OS=Mangifera indica OX=29780 GN=AOMI 1 PE=1 SV=... [more]
O220492.3e-12473.11Ubiquinol oxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AOX2 PE=1 ... [more]
O033761.0e-12161.30Alternative oxidase 3, mitochondrial OS=Glycine max OX=3847 GN=AOX3 PE=1 SV=1[more]
O828072.4e-11864.48Ubiquinol oxidase 1a, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1CI071.2e-200100.00Ubiquinol oxidase OS=Momordica charantia OX=3673 GN=LOC111011576 PE=3 SV=1[more]
G3G3W91.4e-17486.80Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=3 SV=1[more]
A0A6J1JCS22.5e-17487.29Ubiquinol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111483245 PE=3 SV=1[more]
D4P4J83.2e-17486.24Ubiquinol oxidase OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A6J1E6M01.6e-17386.44Ubiquinol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111430458 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64210.11.6e-12573.11alternative oxidase 2 [more]
AT3G22370.14.2e-11870.53alternative oxidase 1A [more]
AT3G27620.12.0e-11567.36alternative oxidase 1C [more]
AT1G32350.13.7e-11472.94alternative oxidase 1D [more]
AT3G22360.13.7e-11469.45alternative oxidase 1B [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 65..102
NoneNo IPR availablePIRSRPIRSR005229-1PIRSR005229-1coord: 173..338
e-value: 1.7E-70
score: 234.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..98
NoneNo IPR availablePANTHERPTHR31803:SF20ALTERNATIVE OXIDASE 3, MITOCHONDRIALcoord: 31..354
IPR002680Alternative oxidasePIRSFPIRSF005229AOcoord: 34..350
e-value: 1.0E-108
score: 361.9
IPR002680Alternative oxidasePFAMPF01786AOXcoord: 144..334
e-value: 2.6E-80
score: 269.1
IPR002680Alternative oxidasePANTHERPTHR31803ALTERNATIVE OXIDASEcoord: 31..354
IPR002680Alternative oxidaseCDDcd01053AOXcoord: 171..338
e-value: 2.31171E-102
score: 296.811
IPR038659Alternative oxidase superfamilyGENE3D1.20.1260.140Alternative oxidasecoord: 120..351
e-value: 2.3E-96
score: 323.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g03220.1Moc10g03220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0070469 respirasome
molecular_function GO:0009916 alternative oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102721 ubiquinol:oxygen oxidoreductase activity