Moc10g03010 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g03010
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectin lyase-like superfamily protein
Locationchr10: 2063338 .. 2069944 (+)
RNA-Seq ExpressionMoc10g03010
SyntenyMoc10g03010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCCATCTTGCCTTAGTTTTCATTTTCTCCATTCATCTTTTAAGCTCATGGGTTGTATCAACTTCGCCCAATTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGCCAAACTGATGATTCACAAGTAAGTAATTTGAGCTATATTCTATTCTCTTTTTCTCCCTTCCAACCTACTGACTCAAATGAAAATCGAACACTTTCGGGTCAAGTCGGTTTTGTCTGAAAATTGAACCGAACCGACTAAATTACACCCCTAACAAAAGTTTTTTTTCTTCGTCTATCTTTGATCAGGCATTTTTGAGAGCTTGGAAGGATGCATGTGGAGCAACGGAAGGAATTCCAGCCTTACATGCACCAGTTGGAAAAATTTATATGTTGAATCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAACAAGTCATTTTCCATGTAATTATTCTTACCCCATTTCTTAATTATAATTTGCCCTTTTCTTACATTTTAAAATTTTTCAATCATTTTTTGTCATATATATATATATTTTTTATTTTTTATTATATGATCCAATATATTATAAGGTAAGAACATGTTTTCACATGATTTTGTTTTTTTGTTTCTTTTAGCTTGGAGGAACGGTAGTGGCTCCAACAAAGGATTCATGGCCAAGTGACGATAGAGATAGATGGATCCAATTTTCAGATATAGACGGTCTCATGGTCAATGGATCAGGACAATTTGATGGCCAAGGTTCTTCTTGGTGGAAAGATTGTGATAAACATTGCACACGCCCAACTGTAAGTCTTATTCGATCATCTTTTATATACTTTCTTGTTTCGTTAGAATTTGATTTTGATTGTTGAATTTATTAATGTGAATATTAATTATAATATATGGTTTTGCAGGCATTATTTTTACACAATTGCAATGGCCTCCAACTTAGAGAAATGAAGCACATTAATAGTGGCAGAAACCATATCTCCATAAATGCTTGTAATGATGCCATTATCTCGGACCTTCACATTATGGCTCCAGAAGATAGCCCTAACACAGATGGAATTGACATTTCTGGATCAAATAATGTTCTTATCCAAAATTCTTTTATGGGAACTGGTAATTAATTAATTAATTAGAAATTTTCAATATTGGACATTTTTATCTTTTGTTGAATTATTTTTATTATTTTTCAAATACTAAACTATATATTATACCAATCGAGTTTACATCAATTCTTTTTTAAATTAAATGTAGGTGACGATTGTATTGCTCTTAACAACGGATCTACCAATATCAACATTGTAGGCGTAACATGTGGCCCCGGCCATGGAATAAGGTAAAAATTAAAGACGTTCTTTTAGTAACTATTTATTGAACAAATATATGTAACATCTAATATTTTTACTTGTAACTTTTATCCATTATCATCTCCTCATAGTCTATCAATTTCAAAAACAAAAAATTAACCATTTTTTTTATCTCCCATTCCAATAAATAAATATTGATTGTTTTGTTTTGTTTGTGAACAGCATTGGGAGCTTAGGAGAAAATGGACAATATAATGTAGTTGAAGACATCCACGTAAAAAATTGTCTCTTAAAGGGCACTCAAAATGGAGTGAGAATCAAGACTTGGCAGGTGATAATTTATTTTCAATATTTATTCATGTACAATAGTATATATAAATATTTATTTAGTATATGTTTTTCTAGCACATAATTATATATCTCTTTTACAGAATGGGTACGGATATGCAAGGGGCATCACATTCGAACAAATAACACTAATAGATGTGAAAAACCCGATCATTATTGATCAGTATTACAGCGATCTTGCAAAAGCCCAAAAAAACAGGGTAAAACTTTTTTTAGTCCCATTATTTTATAGGTCTTTATCTACTATTATGCGTGTGTTTATAAATAATTTATTTGTTTTTTTTGTTGATTTTTTAGTTATTGCCCATAAAAGTGAGCGATGTGACGTATCGTGAAGTTCATGGAACATCAGCAAATGAGAATGCAATTATTTTGAACTGCTCTCGAAGTCGTTGCAGTAATATTATTATGGAAAATGTGAGCATAAAGAACATAGATCCTGGAAAAGAAGCAAAAATTCTTTGTCAAAATGTTGATGGAAGATCTACCTCAGCAATCCCAAGTGTGCCTTGTTTATCAAAGCAGAACTGAATTTTTTATATAATATTATGAGTTGTAATATTGGAGAAAAGGATTTAATGTTCCATCTCCTAATTTATAGTAGGTCTATCTAACAAAGTTTTGGCCTCATTCTAAAAGTCTATTTCTTTTCCATTTTTTATTTCGTATGTTTTTCTTACTCTATTTTATGACTCATTAATTCTTGAGACAATCTCTCAACTAATTGATGAGATTTAAAGATTTGTTGGATCATTATTTTTAAAATTGGAAAAAAAAATTTAGAAATTAAAAGTGTTAGAAAATACACCGGACGACAAGCTAAATCTTTCTACCAAAAATTCGAACATAACTTTTACATTTCAAAGGGGTTTTCAGGAAAGATTAGAGTAGATTTTTAAAATAATTCATAAAATAAGAATTTTTTTTTGAAGTGAAGTATTTATAATATTTAAGAAATTAAAATAGTTCGTCTATATTCATTCAAATAGATCTTTGTTTCAAAATTGTTGCTGTTTTTGCTTTTCAAAAAAAAAAAAAAAGATATTTGTCTCTAAAATGGAAAAAACATTAAAAAGACAAAATACAAACTTCCAAATCAAAAAGTGGCTGAAATGCGAAAAAAATGATTAATGATTAGAAAACACAATTATTATTACAGAAAATACAAAAGTGAAAAAAAAAATCGAAGAAATAATCTTTGAAAAATAAAGAAAAGTTCATATGAAATAAAAACTGATTGTATTTTTTTTATTTAATATCATACACCAATATAATTTGAAACAGGATTGAAATTTTAATTTCAAAATTATATTTAAATTACCATAAATAACATGCTTTTTTTTACAACTAAATAAAATGTTTTCAAATATGGTTCGAAATTAAAAAAATATATATCTATTAATAATTTAATCTTATAAAAATTAAAAGGTCAAATTTATTGTCATGGAGATTCATTTAAGGAAAACATATATTATAATTAGGGGTAGAGTAGGAATTATGTAAAAAAAGTCAAACCCATGATTTTATTGTCAATCCATTTCTGTAAATTTTATAAATTAGTGACATTTATGGAAAATTGGTATAAATTATAGCTAATTGGACACAATCTCTCTCCTATGTCTGAAGTCTGATTATCAAATGTAATATTCTAAAAGAAAACCTCAAATTTCTTTTCCATGTATAACCAGATTCTATCCATCAAATTCATTACTATAAAATAAAGAGATTCTATTCATCAATTGTATTATTTAATATCCAAATAAAATGGATGCATGATGAATAATACTCATAAAAAAAGGGTAAATAAGTAATTCTCATAATATGGATATTTAATATCTAACTTTTTGTAACAAAATCAAGAGATTCACGTGTGATTTTTCTTTCCATTAAAAAATTTGACTTATTTTTCTTATTATTATTTTATAGTAAAATAGATGGGGTAAAAATTTTAACTTATCATCTTCGAAGATAGATATCGGACAAAATTTGACTTATTTGAAGACAAACAGATAACTAAATTAATTAATACATTATTTTTGTTAGTTTAAGCCTCTAGTATTGAGCAACGAACACAAATTTCTTAAAATATAAATTATTACGTAATACAAAGAAAATGTTAATTCTTGCTTAGATGAGCCATAAGACATTTCTTTGATAAAAAAAAAAATAAGTTAATTAAAATAATGTTAATTATTAACTAACATAATTGAATGAGAGATACAGACGAACTAACATTTTTCGTTTCTGTTTTGTCTTTTGTCTTCTTTTTTTTTTTTTCCATCTCCATATTCTCAATTATTCATTTCAGATAACTCTAGTTCTTGAGGAAGAAAAAAACATATCCACTCAACAACATTATAATAATTTTATTACACAATCTAATTCATCAATATTAAGTTACCCAAAAAACTATTGAAACACATCCAACAAAATCAAGCTTGAATTTCCATAAATTTGTGGCAGATTGACTCACGGTTTGACATCACTTACCATATTTATGCACAAAAACCATTAATTTCTCCGAAACAAAATCTCCCATAAAAACAAAAAAAAACTGATAATAATGATTAAAATAATATGCTTGACCCATTAATTAATAATAGATTTGAGAAAAATCCTCCATTTTTTATGCATACCAATATGGACACATTGCTTCTTTCAAGCCTTATAATTATATATCTGATTAATATTTTCCTTTCTAATCTTCAATACTTACAAAATATTAAAAAAGAAAAACAATTTTACAATTCCCATTTCACCCCTTCATCCTCTATATAAGCATGCCATTTATTTTCATCCTTCATTATCCTTCCAAAATTTAGCACGATCTTCTTCTTTTTGTTTATCAAAATCCGCCATTTTATCTTAGAAAATGAAGCTCCATTTTGTCTTACTTTTCATCTTCTCCATTAATATTTTGAGCTCGTGGATTGTGTCAGCTTCACCCAGCTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGGCAAGCTGATGATTCACAAGTAAAATAATTTAATCTACATTTTGGACTACTTTTAATTCTTTCTTTATCTTCTTCTTTATATAATTGTTTTTCTTTCTTTTTTATTTTTTTATAAGGCTTTTTTGAGAGCTTGGAATGATGTCTGTGGAGCAACAGAAGGAATTCCAACATTACATGCACCACATGGAAAAATTTATATGTTGAAGCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAACAAGTTACTTTCCATGTAAATTTCTTGCTCATCATTTTTTTTATTATATGTCTGGACATATCAAGCTTAGAAAATATTGTTATGGTTTTGTGATTTTGTAGCTTAAAGGGACTCTAGTGGCTCCAAAAAAAGGTTCATGGCCAAAGTACGATAGAGAGAGATGGATTCAATTTTCAGACATAGATGGTCTCATTGTCAATGGAACAGGACAATTTGATGGTCAAGGCTCCTCTTGGTGGGAAGATTGGGAACACTGTCAACGACCAACCGTAAGTTTTATTCGATCATCTTTCCATCGTCTTATCTTACTATACATATTATATATAAAAGTGTAGTGTGCTTAAAGAGTTTTTATGAGTGAAATGAAAAGCTAAGGTAAAAAAGTACCAGATAACCCATGTACGTCTAATCAAATAAATTTTTACTACATATTATATATAAAATTGTGGTCTACTCAGAGGGAATTTAGGAGTAAAATAAAAATATATGAAAAAAGGTACCAAATAACACTTGATAGATTTTATCCTAACTAGAATTTGATTTTAATGGTTGAATTTATAATGTATGGTTTTGCAGGCACTATTTTTGCACAATTGCAATGGTCTCCAACTAAGGGAAATGAAGCATATTAATAGTGCCAAAAATCATATCTCCATAAATGCTTGTAATGATGTCATTATCTCAGACCTTCACATTATTGCTCCTGAAGATAGCCCTAACACAGATGGAATTGATGTTTCTAGATCAAACAATGTTCTTATTCAAAATTCTTTCATGGGAACAGGTAATTAATTAATTAATTAGCATTTTTCATGATCATTTTTTATTCTTTTGTTTATTACATACATTCAAGGTTAAAATAACCAATTTTATTAATTTCATACTAATTTCTATATGTAGGTGACGATTGTATTGCCCTTAACAATGGATCTTCCAATATCGATATTGTAGGTGTAACCTGTGGCCCAGGCCATGGCATAAGGTAAAAATGATGTTTTCTAGTAACAGTAAATAATTACTATTACCAAAAAAAATTACATTTGACCATTTTTATCTCTCATTCCTTATATATATTTTTTATTTTTGTATTTATTTGAAAACAGTATTGGGAGCTTGGGAGGAAATAGAGAATACAACGTAGTTGAAAACATTCATGTAAAAAATTGCCTCTTAAAGGGCACTCAAAATGGAATGAGAATCAAGACTTGGCAGGTGATAATTGTTTCTAGCTCAATTTATTATTATAAAATAATGTTTGTAAATAAATTTCTAGCACATAACTTTTTGTTTTTAATAACGTTTTGAGTTTATAGGGTGGATATGGATATGCAAGGAACATCACATTCGAACAAATAACACTAATGGGTGCGAAAAACCCGATCATTATTGATCAATATTATACCGGTCTTGCAAAGGCACAAAAAAACAAGGTAAAATATTTGGTGTAGTTCTCATTAATTTATCTACTGTTATCACTGCTTTTATATAATCTATAAATTTCTTTTCAATATTTTTTCAGTTATTCGACATAAAAGTGAGCAACGTGACTTATCGTGAAGTTAATGGAACGTCAGCAAATGAGAATGCAATTATTTTGAACTGCTCTCGAAATCGCTGCAGCAATATTATTTTGGAAAATGTGAGCATAAAACCAATAGATCCAAAAATGGAAGCCAAAGCTTTTTGTCAAAATGTTGATGGAAGATCTACTTATGCAGTTCCAACCGTTCCTTGTTTATCAAATTAG

mRNA sequence

ATGAAGCTCCATCTTGCCTTAGTTTTCATTTTCTCCATTCATCTTTTAAGCTCATGGGTTGTATCAACTTCGCCCAATTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGCCAAACTGATGATTCACAACTTGGAGGAACGGTAGTGGCTCCAACAAAGGATTCATGGCCAAGTGACGATAGAGATAGATGGATCCAATTTTCAGATATAGACGGTCTCATGGTCAATGGATCAGGACAATTTGATGGCCAAGGTTCTTCTTGGTGGAAAGATTGTGATAAACATTGCACACGCCCAACTGCATTATTTTTACACAATTGCAATGGCCTCCAACTTAGAGAAATGAAGCACATTAATAGTGGCAGAAACCATATCTCCATAAATGCTTGTAATGATGCCATTATCTCGGACCTTCACATTATGGCTCCAGAAGATAGCCCTAACACAGATGGAATTGACATTTCTGGATCAAATAATGTTCTTATCCAAAATTCTTTTATGGGAACTGGTGACGATTGTATTGCTCTTAACAACGGATCTACCAATATCAACATTGTAGGCGTAACATGTGGCCCCGGCCATGGAATAAGCATTGGGAGCTTAGGAGAAAATGGACAATATAATGTAGTTGAAGACATCCACGTAAAAAATTGTCTCTTAAAGGGCACTCAAAATGGAGTGAGAATCAAGACTTGGCAGAATGGGTACGGATATGCAAGGGGCATCACATTCGAACAAATAACACTAATAGATGTGAAAAACCCGATCATTATTGATCAGTATTACAGCGATCTTGCAAAAGCCCAAAAAAACAGGTTATTGCCCATAAAAGTGAGCGATGTGACGTATCGTGAAGTTCATGGAACATCAGCAAATGAGAATGCAATTATTTTGAACTGCTCTCGAAGTCGTTGCAGTAATATTATTATGGAAAATGTGAGCATAAAGAACATAGATCCTGGAAAAGAAGCAAAAATTCTTTGTCAAAATGTTGATGGAAGATCTACCTCAGCAATCCCAACTTCACCCAGCTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGGCAAGCTGATGATTCACAACTTAAAGGGACTCTAGTGGCTCCAAAAAAAGGTTCATGGCCAAAGTACGATAGAGAGAGATGGATTCAATTTTCAGACATAGATGGTCTCATTGTCAATGGAACAGGACAATTTGATGGTCAAGGCTCCTCTTGGTGGGAAGATTGGGAACACTGTCAACGACCAACCGCACTATTTTTGCACAATTGCAATGGTCTCCAACTAAGGGAAATGAAGCATATTAATAGTGCCAAAAATCATATCTCCATAAATGCTTGTAATGATGTCATTATCTCAGACCTTCACATTATTGCTCCTGAAGATAGCCCTAACACAGATGGAATTGATGTTTCTAGATCAAACAATGTTCTTATTCAAAATTCTTTCATGGGAACAGGTGACGATTGTATTGCCCTTAACAATGGATCTTCCAATATCGATATTGTAGGTGTAACCTGTGGCCCAGGCCATGGCATAAGTATTGGGAGCTTGGGAGGAAATAGAGAATACAACGTAGTTGAAAACATTCATGTAAAAAATTGCCTCTTAAAGGGCACTCAAAATGGAATGAGAATCAAGACTTGGCAGGGTGGATATGGATATGCAAGGAACATCACATTCGAACAAATAACACTAATGGGTGCGAAAAACCCGATCATTATTGATCAATATTATACCGGTCTTGCAAAGGCACAAAAAAACAAGTTATTCGACATAAAAGTGAGCAACGTGACTTATCGTGAAGTTAATGGAACGTCAGCAAATGAGAATGCAATTATTTTGAACTGCTCTCGAAATCGCTGCAGCAATATTATTTTGGAAAATGTGAGCATAAAACCAATAGATCCAAAAATGGAAGCCAAAGCTTTTTGTCAAAATGTTGATGGAAGATCTACTTATGCAGTTCCAACCGTTCCTTGTTTATCAAATTAG

Coding sequence (CDS)

ATGAAGCTCCATCTTGCCTTAGTTTTCATTTTCTCCATTCATCTTTTAAGCTCATGGGTTGTATCAACTTCGCCCAATTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGCCAAACTGATGATTCACAACTTGGAGGAACGGTAGTGGCTCCAACAAAGGATTCATGGCCAAGTGACGATAGAGATAGATGGATCCAATTTTCAGATATAGACGGTCTCATGGTCAATGGATCAGGACAATTTGATGGCCAAGGTTCTTCTTGGTGGAAAGATTGTGATAAACATTGCACACGCCCAACTGCATTATTTTTACACAATTGCAATGGCCTCCAACTTAGAGAAATGAAGCACATTAATAGTGGCAGAAACCATATCTCCATAAATGCTTGTAATGATGCCATTATCTCGGACCTTCACATTATGGCTCCAGAAGATAGCCCTAACACAGATGGAATTGACATTTCTGGATCAAATAATGTTCTTATCCAAAATTCTTTTATGGGAACTGGTGACGATTGTATTGCTCTTAACAACGGATCTACCAATATCAACATTGTAGGCGTAACATGTGGCCCCGGCCATGGAATAAGCATTGGGAGCTTAGGAGAAAATGGACAATATAATGTAGTTGAAGACATCCACGTAAAAAATTGTCTCTTAAAGGGCACTCAAAATGGAGTGAGAATCAAGACTTGGCAGAATGGGTACGGATATGCAAGGGGCATCACATTCGAACAAATAACACTAATAGATGTGAAAAACCCGATCATTATTGATCAGTATTACAGCGATCTTGCAAAAGCCCAAAAAAACAGGTTATTGCCCATAAAAGTGAGCGATGTGACGTATCGTGAAGTTCATGGAACATCAGCAAATGAGAATGCAATTATTTTGAACTGCTCTCGAAGTCGTTGCAGTAATATTATTATGGAAAATGTGAGCATAAAGAACATAGATCCTGGAAAAGAAGCAAAAATTCTTTGTCAAAATGTTGATGGAAGATCTACCTCAGCAATCCCAACTTCACCCAGCTTCAATGTTCTTGATTATGGAGCCATTGGAAATGGGCAAGCTGATGATTCACAACTTAAAGGGACTCTAGTGGCTCCAAAAAAAGGTTCATGGCCAAAGTACGATAGAGAGAGATGGATTCAATTTTCAGACATAGATGGTCTCATTGTCAATGGAACAGGACAATTTGATGGTCAAGGCTCCTCTTGGTGGGAAGATTGGGAACACTGTCAACGACCAACCGCACTATTTTTGCACAATTGCAATGGTCTCCAACTAAGGGAAATGAAGCATATTAATAGTGCCAAAAATCATATCTCCATAAATGCTTGTAATGATGTCATTATCTCAGACCTTCACATTATTGCTCCTGAAGATAGCCCTAACACAGATGGAATTGATGTTTCTAGATCAAACAATGTTCTTATTCAAAATTCTTTCATGGGAACAGGTGACGATTGTATTGCCCTTAACAATGGATCTTCCAATATCGATATTGTAGGTGTAACCTGTGGCCCAGGCCATGGCATAAGTATTGGGAGCTTGGGAGGAAATAGAGAATACAACGTAGTTGAAAACATTCATGTAAAAAATTGCCTCTTAAAGGGCACTCAAAATGGAATGAGAATCAAGACTTGGCAGGGTGGATATGGATATGCAAGGAACATCACATTCGAACAAATAACACTAATGGGTGCGAAAAACCCGATCATTATTGATCAATATTATACCGGTCTTGCAAAGGCACAAAAAAACAAGTTATTCGACATAAAAGTGAGCAACGTGACTTATCGTGAAGTTAATGGAACGTCAGCAAATGAGAATGCAATTATTTTGAACTGCTCTCGAAATCGCTGCAGCAATATTATTTTGGAAAATGTGAGCATAAAACCAATAGATCCAAAAATGGAAGCCAAAGCTTTTTGTCAAAATGTTGATGGAAGATCTACTTATGCAGTTCCAACCGTTCCTTGTTTATCAAATTAG

Protein sequence

MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQLGGTVVAPTKDSWPSDDRDRWIQFSDIDGLMVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVKNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENVSIKNIDPGKEAKILCQNVDGRSTSAIPTSPSFNVLDYGAIGNGQADDSQLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKAQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQNVDGRSTYAVPTVPCLSN
Homology
BLAST of Moc10g03010 vs. NCBI nr
Match: XP_022141108.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 677.2 bits (1746), Expect = 1.5e-190
Identity = 340/390 (87.18%), Postives = 341/390 (87.44%), Query Frame = 0

Query: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQ---------------- 60
           MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQ                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120
                                         LGGTVVAPTKDSWPSDDRDRWIQFSDIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180
           MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240
           IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300
           HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 345
           NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

BLAST of Moc10g03010 vs. NCBI nr
Match: XP_022141107.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 629.4 bits (1622), Expect = 3.5e-176
Identity = 316/377 (83.82%), Postives = 319/377 (84.62%), Query Frame = 0

Query: 336 STSAIPTSPSFNVLDYGAIGNGQADDSQ-------------------------------- 395
           S+  +  SPSFNVLDYGAIGNGQADDSQ                                
Sbjct: 17  SSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGIPTLHAPHGKIYMLKPL 76

Query: 396 --------------LKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW 455
                         LKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW
Sbjct: 77  KFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW 136

Query: 456 EDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTD 515
           EDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTD
Sbjct: 137 EDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTD 196

Query: 516 GIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVV 575
           GIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVV
Sbjct: 197 GIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVV 256

Query: 576 ENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKAQK 635
           ENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYT       
Sbjct: 257 ENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTDFFSI-F 316

Query: 636 NKLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQN 667
            +LFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQN
Sbjct: 317 FQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQN 376

BLAST of Moc10g03010 vs. NCBI nr
Match: KAA8540480.1 (hypothetical protein F0562_024601 [Nyssa sinensis])

HSP 1 Score: 600.1 bits (1546), Expect = 2.3e-167
Identity = 326/709 (45.98%), Postives = 441/709 (62.20%), Query Frame = 0

Query: 1   MKLHLALVFIFSIH----LLSSWVVSTSPNFNVLDYGAIGNGQTDDSQLGGTVVAPTKDS 60
           M++HL +  I  +      LS+ ++  S +FNV++YGA+G+G TDDSQ            
Sbjct: 1   MQVHLTIFLILCMASSGLSLSTNLIILSTSFNVMNYGAVGDGSTDDSQ------------ 60

Query: 61  WPSDDRDRWIQFSDIDGLMVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREM 120
                                                   C  P AL  + CNGL+L  +
Sbjct: 61  -------------------------------------GAQCNVPAALSFNRCNGLRLSGL 120

Query: 121 KHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIA 180
            HINS R+HI+I   N AIIS+L I+APE SPNTDGIDIS S+ V I+N  +GTGDDCIA
Sbjct: 121 THINSPRSHITITKSNGAIISNLRIIAPETSPNTDGIDISSSSQVQIRNCIIGTGDDCIA 180

Query: 181 LNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNG 240
           +  GS+NINI GVTCGPGHGISIG+LG+ G Y+ VE+IHV+NC  KGT NGVRIKTWQ G
Sbjct: 181 IGGGSSNINITGVTCGPGHGISIGALGQAG-YDRVEEIHVRNCTFKGTMNGVRIKTWQGG 240

Query: 241 YGYARGITFEQITLIDVKNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENA 300
            GYAR I+FE+I  I   NPI IDQ+Y       +N+   IK+SD++Y  + GTS  +N 
Sbjct: 241 NGYARKISFEKIKFIAAGNPIFIDQFYCPSQVNCQNKTSAIKLSDISYTGIIGTSTVDNV 300

Query: 301 IILNCSRS-RCSNIIMENVSIKNIDPGKEAKILCQNVDGR-------------------- 360
           I L+CS+S  C+NI++++V I +  PG      C +  GR                    
Sbjct: 301 INLSCSQSVGCTNIVLDHVYITSTTPGNRVYANCFDAHGRASHTRPADYLTVFLILCMAS 360

Query: 361 -----STSAIPTSPSFNVLDYGAIGNGQADDSQLKGTLVAP-KKGSWPKYDRERWIQFSD 420
                +T+ + ++ SF+V++YGA+G+G  DDSQ+ G +VAP +K +W  Y   RW+ FS+
Sbjct: 361 SGLSTTTNLVSSTSSFDVMNYGAVGDGTTDDSQVSGNIVAPNEKSAWTGYHINRWLTFSN 420

Query: 421 IDGLIVNGTGQFDGQGSSWW-----EDWEH--------CQRPTALFLHNCNGLQLREMKH 480
           I+GLI+NG GQ DG+GS+WW     E+ ++        C+ PTAL  + CNGL+L  + H
Sbjct: 421 INGLIINGRGQIDGKGSAWWPQPCLENVQYDTICKGPTCKGPTALTFNRCNGLRLDGLTH 480

Query: 481 INSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALN 540
           INS ++HI +  CN  IIS+LHIIAPE SPNTDGID+  S N+ I+N  +GTGDDCIA+ 
Sbjct: 481 INSPRSHIVLTNCNSSIISNLHIIAPETSPNTDGIDIYGSTNIQIRNCNIGTGDDCIAIC 540

Query: 541 NGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYG 600
            GSSN+DI GV CGPGHGISIG+L G+  Y+ VENI V+NC L+GT NG+RIK+WQGG G
Sbjct: 541 GGSSNVDIAGVICGPGHGISIGAL-GHGGYDAVENIKVRNCTLRGTTNGVRIKSWQGGNG 600

Query: 601 YARNITFEQITLMGAKNPIIIDQYYTGLAKAQKNKLFDIKVSNVTYREVNGTSANENAII 660
           YAR I+FE+I  + A NPIIIDQ+Y       +NK   IK+S+++Y  ++GTS ++  I 
Sbjct: 601 YARKISFEKINFIAASNPIIIDQFYCPPGVDCQNKTSAIKLSDISYTAISGTSTSDKVID 658

Query: 661 LNCSRN-RCSNIILENVSIKPIDPKMEAKAFCQNVDGRSTYAVPTVPCL 665
           L+CS++  C+NI+L++V I P        A C N  GR+++  P V CL
Sbjct: 661 LSCSQSVGCTNIVLDHVYITPTVQGKRIYANCFNAHGRASHTKPAVKCL 658

BLAST of Moc10g03010 vs. NCBI nr
Match: KAF5196806.1 (Polygalacturonase adpg1 [Thalictrum thalictroides])

HSP 1 Score: 595.5 bits (1534), Expect = 5.6e-166
Identity = 326/770 (42.34%), Postives = 443/770 (57.53%), Query Frame = 0

Query: 6    ALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQ--------------------- 65
            ++  I S   L   +  +  + +VLD+GA+G+G TDDSQ                     
Sbjct: 543  SVFIIISTIFLFLCLAPSGMSSSVLDFGAVGDGNTDDSQAFLKAWQTICESAEVRPTLII 602

Query: 66   -------------------------LGGTVVAPTKDSWP-SDDRDRWIQFSDIDGLMVNG 125
                                     + G +VAP   SWP + D + W++F+D+ GL+V G
Sbjct: 603  PAGKTFFLNPVSFEGPCKPANIRVWILGNLVAPVMSSWPKTADVNEWLKFADVSGLIVAG 662

Query: 126  SGQFDGQGSSWWKDCDKH--------CTRPTALFLHNCNGLQLREMKHINSGRNHISINA 185
             GQ +G+G +WW DC +         C RPTAL  ++C  L+L ++  INS  NHISIN 
Sbjct: 663  RGQINGRGETWW-DCGRKISLCKLDGCNRPTALKFNDCTDLKLSDITSINSQSNHISINE 722

Query: 186  CNDAIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVT 245
            C  A I+++H++APEDS NTDGIDIS SNNV+IQ+SF+ TGDDC+A+N GS+ INI    
Sbjct: 723  CKGATITNVHLIAPEDSNNTDGIDISKSNNVVIQDSFIATGDDCVAINGGSSFINITNTA 782

Query: 246  CGPGHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITL 305
            CGPGHGIS+GSLGENG  ++VE IHVKNC   GT NGVRIKTW  G GYA+ +TFEQI  
Sbjct: 783  CGPGHGISVGSLGENGDSSIVEGIHVKNCSFTGTTNGVRIKTWPFGTGYAKKLTFEQIRF 842

Query: 306  IDVKNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRS-RCSNI 365
             +VKNPIIIDQYY         +   I+VSDVTY  VHGTS    AI LNCS +  C+NI
Sbjct: 843  ENVKNPIIIDQYYCPDDNC-GGKSSAIQVSDVTYIGVHGTSVMPTAITLNCSATVPCNNI 902

Query: 366  IMENVSIKNIDPGKEAKILCQNVDGRSTSAIPT------------------------SPS 425
            +M +V+IK+ + G     +C N  G+ +   PT                        + +
Sbjct: 903  VMNDVNIKSTE-GIPTTAICNNAHGKESLTDPTEFWLIFMFLCLFPFSWSSDLDAMITNN 962

Query: 426  FNVLDYGAIGNGQADDSQ------------------------------------------ 485
            FNV+ YGA+G+G+ DDSQ                                          
Sbjct: 963  FNVISYGAVGDGKTDDSQAFLNAWKAMCFAKFGIVFLTIPPSKTFLSRPLNFRGPCKSPI 1022

Query: 486  ----LKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW----EDWEHC 545
                ++G ++AP+   W   D+ RW+ FSD++GLIV G+G+ DG G SWW    +    C
Sbjct: 1023 VKVSVEGNIIAPEMSKWNNGDKSRWLTFSDVNGLIVTGSGRIDGNGQSWWVCAGKSGSGC 1082

Query: 546  QRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSR 605
             RP AL  H+CN L +  +  +NS KNHI+IN C    IS++HII+P+ SPNTDGI++S 
Sbjct: 1083 SRPAALTFHDCNNLDVSGLNIVNSPKNHITINDCKGAKISNIHIISPDSSPNTDGINLSN 1142

Query: 606  SNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVK 645
              NV I++S + +GDDCIA+N+G S ++I  V CGPGHGIS+GSLG N ++  VE IHV+
Sbjct: 1143 VMNVQIRDSIIKSGDDCIAINDGCSFVNITNVGCGPGHGISVGSLGANGDHCAVEEIHVR 1202

BLAST of Moc10g03010 vs. NCBI nr
Match: XP_023920973.1 (uncharacterized protein LOC112032446 [Quercus suber])

HSP 1 Score: 578.2 bits (1489), Expect = 9.3e-161
Identity = 322/757 (42.54%), Postives = 425/757 (56.14%), Query Frame = 0

Query: 5   LALVFIFSIH---LLSSWVVSTSPNFNVLDYGAIGNGQTDDS------------------ 64
           L  V I SI    L    V  TS  FNVL+YGA+G+GQTDDS                  
Sbjct: 15  LTFVLILSISSTCLCRGLVEDTSGGFNVLNYGAVGDGQTDDSQAFLKAWTALCQTKGTPQ 74

Query: 65  ---------------------------QLGGTVVAP-TKDSWPSDDRDRWIQFSDIDGLM 124
                                      Q+ GT++AP + D W +   + W++FS +  L+
Sbjct: 75  LTVPSGKKFLLMPVLFQGPCQSNSVQVQILGTMLAPNSMDGWTNCSTNCWLRFSGVSNLI 134

Query: 125 VNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDAI 184
           VNGSG  DG+GS+WW           AL  ++CN LQL  + H+NS RNHIS+ ACN A 
Sbjct: 135 VNGSGTIDGRGSTWW----NQVYNDQALQFNSCNNLQLSGLTHLNSPRNHISLTACNGAS 194

Query: 185 ISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPGH 244
           +S LHI AP  SPNTDGIDIS S N+ I +S + TGDDCIA+N  S +INI G+ CGPGH
Sbjct: 195 LSYLHISAPASSPNTDGIDISSSTNINIHDSNIATGDDCIAINGNSAHINITGINCGPGH 254

Query: 245 GISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVKN 304
           GIS+GSLG NG +  VE ++V++C    TQNG RIKTW  G GYAR I F+ I L    N
Sbjct: 255 GISVGSLGRNGAHETVEYVYVQHCNFTQTQNGARIKTWPGGSGYARYIYFQHIQLQQASN 314

Query: 305 PIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENVS 364
           PIIIDQ+Y    +    +   ++VS+VTY++++GTS N+  II NC +      ++  V 
Sbjct: 315 PIIIDQFYCGGGQGCTGKSSAVQVSNVTYKDINGTSGNQKGIIFNCDQEE----LLTFVL 374

Query: 365 IKNIDPGKEAKILCQNVDGRSTSAIPTSPSFNVLDYGAIGNGQADDS------------- 424
           I +I        LC+ +         TS  FNVLDYGAIG+GQ DDS             
Sbjct: 375 ILSI----SLTCLCRGL------VEDTSGGFNVLDYGAIGDGQTDDSQAFSKAWTALCQT 434

Query: 425 --------------------------------QLKGTLVAPKK-GSWPKYDRERWIQFSD 484
                                           Q+ GT++AP     W       W++FS 
Sbjct: 435 KGTPQLIIPSGKKFLLMPVMFQGPCQSNSVQVQILGTMLAPNSMDGWTNCSANCWLRFSG 494

Query: 485 IDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINAC 544
           +  LIVNG+G  DG+GS+WW          AL  + CN LQL  + H+NS +NHIS+ AC
Sbjct: 495 VSNLIVNGSGTIDGRGSTWWNQ--------ALQFNYCNNLQLSGLTHLNSPRNHISLTAC 554

Query: 545 NDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTC 604
           N   +S LHI AP  SPNTDGID+S S N+ I +S + TGDDCIA+N  S++I+I G+ C
Sbjct: 555 NGASLSYLHISAPASSPNTDGIDISSSTNINIHDSNIATGDDCIAINGNSAHINITGINC 614

Query: 605 GPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLM 664
           GPGHGIS+GSLG N  +  VE ++V++C    TQNG RIKTW GG GYAR I F+ I L 
Sbjct: 615 GPGHGISVGSLGRNGAHETVEYVYVQHCNFTQTQNGARIKTWPGGSGYARYIYFQHIQLQ 674

Query: 665 GAKNPIIIDQYYTGLAKAQKNKLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIIL 667
            A NPIIIDQ+Y    +    K   ++VS+VTY ++ GTS N+  II NC +  C+NII+
Sbjct: 675 QASNPIIIDQFYCDGGQGCTGKSSAVQVSDVTYIDIKGTSGNQQGIIFNCDQEVCTNIIM 734

BLAST of Moc10g03010 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 4.9e-80
Identity = 159/307 (51.79%), Postives = 202/307 (65.80%), Query Frame = 0

Query: 363 QLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALF 422
           Q+ G LVAP KG+W K D+++WI F+DI+GL++ G G+ +GQGSSWWE      RPTAL 
Sbjct: 86  QMLGKLVAPSKGNW-KGDKDQWILFTDIEGLVIEGDGEINGQGSSWWE--HKGSRPTALK 145

Query: 423 LHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQ 482
             +CN L+L  + H++S   HI I+ CN V IS L I APE SPNTDGIDV  S+NV+IQ
Sbjct: 146 FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQ 205

Query: 483 NSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGT 542
           +  + TGDDCIA+N+G+SNI I G+ CGPGHGISIGSLG + E   VEN+ V+NC  +GT
Sbjct: 206 DCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGT 265

Query: 543 QNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG--LAKAQKNKLFDIKVSNV 602
            NG RIKTWQGG GYAR ITF  ITL   +NPIIIDQ+Y G     A+  K   ++VS V
Sbjct: 266 MNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKV 325

Query: 603 TYREVNGTSANENAIILNCS-RNRCSNIILENVSIKPID--PKMEAKAFCQNVDGRSTYA 662
            +    GTS +E  +   CS R  C+ I L ++ I+         A+  C NV G ST A
Sbjct: 326 VFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIA 385

Query: 663 VPTVPCL 665
           VP + CL
Sbjct: 386 VPGLECL 389

BLAST of Moc10g03010 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 5.5e-63
Identity = 125/313 (39.94%), Postives = 183/313 (58.47%), Query Frame = 0

Query: 363 QLKGTLVAPKKGS-WPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWE-DWEH-----C 422
           Q+ GT++AP     W   +R +W+ F  +  L V G G  +G G  WWE   +H     C
Sbjct: 114 QISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPC 173

Query: 423 Q-RPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVS 482
           +  PTAL  H C  +++  +  I+S + HI++ +C  V IS L +IAP  SPNTDGI +S
Sbjct: 174 RGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHIS 233

Query: 483 RSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHV 542
            S  ++I N+ + TGDDCI++   S+ I I  + CGPGHGISIGSLG ++ +  V +I V
Sbjct: 234 VSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITV 293

Query: 543 KNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKAQKNKLFD 602
              ++  T NG+RIKTWQGG G    I F  I +    NPIIIDQYY    K   N+   
Sbjct: 294 DTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSA 353

Query: 603 IKVSNVTYREVNGTSANENAIILNCS-RNRCSNIILENVSIKPIDPKMEAKAFCQNVDGR 662
           I + N+++  V GTSA++ AI ++CS  + C NI+L+++ ++P +     ++FC    G 
Sbjct: 354 ISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGS 413

Query: 663 STYAVPTVPCLSN 667
           S+  V   PCLS+
Sbjct: 414 SSGQVYPPPCLSD 426

BLAST of Moc10g03010 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 6.0e-62
Identity = 133/301 (44.19%), Postives = 184/301 (61.13%), Query Frame = 0

Query: 363 QLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNG--TGQFDGQGSSWWEDWEHCQR--- 422
           Q+ GTL A +K S  K D  +WI F  ++ L V+G  TG  DG G +WW++   C+R   
Sbjct: 129 QIFGTLSASQKRSDYK-DISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQN--SCKRNKA 188

Query: 423 ------PTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGI 482
                 PTAL  +N   L ++ +K  N+ +  ISI  C++V +S++ + AP DSPNTDGI
Sbjct: 189 KPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGI 248

Query: 483 DVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVEN 542
            ++ + N+ +  S +GTGDDCI++ +GS N+ I  +TCGPGHGISIGSLG +     V  
Sbjct: 249 HITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSG 308

Query: 543 IHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKAQKNK 602
           + V    L GT NG+RIKT+QGG G A NI F+ I +   KNPIIIDQ Y   +K    K
Sbjct: 309 VTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEK 368

Query: 603 LFDIKVSNVTYREVNGTSANENAIILNCSRN-RCSNIILENVSIKPIDPKMEAKAFCQNV 652
              ++V NV YR+++GTSA+ENAI  NCS+N  C  I+L+ V+IK        KA C N 
Sbjct: 369 -SAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK------GGKATCTNA 419

BLAST of Moc10g03010 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 6.2e-59
Identity = 124/311 (39.87%), Postives = 178/311 (57.23%), Query Frame = 0

Query: 363 QLKGTLVAPK-KGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQR---- 422
           +++GT+ A   +  W  ++R+RWI+F DI  L + G G  +G G +WW+    C+R    
Sbjct: 143 KIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWD--SSCKRKKSL 202

Query: 423 -----PTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGID 482
                PTAL   +C  L + ++   +S K H+S + C DVI S+L + APE SPNTDGI 
Sbjct: 203 PCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIH 262

Query: 483 VSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENI 542
           ++ +  + + NS +GTGDDCI++ +GS  +    +TCGPGHGISIGSLG       V  +
Sbjct: 263 ITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGV 322

Query: 543 HVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKAQKNKL 602
            V    L  T NG+RIKTWQGG G A+NI F+ I +    NPIIIDQYY         + 
Sbjct: 323 LVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQE 382

Query: 603 FDIKVSNVTYREVNGTSANENAIILNCSRNR-CSNIILENVSIKPIDPKMEAKAFCQNV- 657
             +KVSNV Y  + GTSA+E A+  +CS++  C   I+ N+++     K E    C N+ 
Sbjct: 383 SAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGK-ETTMSCSNIV 442

BLAST of Moc10g03010 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.4e-58
Identity = 129/320 (40.31%), Postives = 182/320 (56.88%), Query Frame = 0

Query: 44  QLGGTVVA-PTKDSWPSDDRDRWIQFSDIDGLMVNGSGQFDGQGSSWWKDCDKHCT---- 103
           ++ GT+ A P    W +     W+ F+ +    + G+G  DGQG+ WW D  K       
Sbjct: 120 KVDGTIAAYPDPAKWKNS--KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGRTV 179

Query: 104 -----RPTALFLHNCNGLQLREMKHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGI 163
                RPTA+ +     + ++E+   NS   H+    C+   I  + I AP DSPNTDGI
Sbjct: 180 CNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPNTDGI 239

Query: 164 DISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVED 223
           DI  S    I+   +GTGDDC+A+  GS+NI I  +TCGPGHG+SIGSLG+    + V  
Sbjct: 240 DIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRSEVSF 299

Query: 224 IHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVKNPIIIDQYYSDLAKAQKNR 283
           +H+       TQNG+RIKTWQ G G A  IT+E + +I+ +NPI+I+Q+Y   A A KN+
Sbjct: 300 VHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSAAACKNQ 359

Query: 284 LLPIKVSDVTYREVHGTSANENAIILNCSRS-RCSNIIMENVSIKNIDPGKEAKILCQNV 343
              +K+ DVT++ +HGTSA   AI L CS S  CSNI + NV +K +  GK A  + +N 
Sbjct: 360 RSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVFLK-LTSGKVATCVNKNA 419

Query: 344 DGRSTSAIPTSPSFNVLDYG 353
           +G  T+  P +PS   L  G
Sbjct: 420 NGYYTN--PLNPSCKSLHPG 434

BLAST of Moc10g03010 vs. ExPASy TrEMBL
Match: A0A6J1CJJ6 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011582 PE=3 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 7.1e-191
Identity = 340/390 (87.18%), Postives = 341/390 (87.44%), Query Frame = 0

Query: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQ---------------- 60
           MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQ                
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  ------------------------------LGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120
                                         LGGTVVAPTKDSWPSDDRDRWIQFSDIDGL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180
           MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240
           IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300
           HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 345
           NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

BLAST of Moc10g03010 vs. ExPASy TrEMBL
Match: A0A6J1CIX8 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011581 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.7e-176
Identity = 316/377 (83.82%), Postives = 319/377 (84.62%), Query Frame = 0

Query: 336 STSAIPTSPSFNVLDYGAIGNGQADDSQ-------------------------------- 395
           S+  +  SPSFNVLDYGAIGNGQADDSQ                                
Sbjct: 17  SSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGIPTLHAPHGKIYMLKPL 76

Query: 396 --------------LKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW 455
                         LKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW
Sbjct: 77  KFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW 136

Query: 456 EDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTD 515
           EDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTD
Sbjct: 137 EDWEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTD 196

Query: 516 GIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVV 575
           GIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVV
Sbjct: 197 GIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVV 256

Query: 576 ENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTGLAKAQK 635
           ENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYT       
Sbjct: 257 ENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTDFFSI-F 316

Query: 636 NKLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQN 667
            +LFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQN
Sbjct: 317 FQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENVSIKPIDPKMEAKAFCQN 376

BLAST of Moc10g03010 vs. ExPASy TrEMBL
Match: A0A5J5BDR4 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_024601 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 326/709 (45.98%), Postives = 441/709 (62.20%), Query Frame = 0

Query: 1   MKLHLALVFIFSIH----LLSSWVVSTSPNFNVLDYGAIGNGQTDDSQLGGTVVAPTKDS 60
           M++HL +  I  +      LS+ ++  S +FNV++YGA+G+G TDDSQ            
Sbjct: 1   MQVHLTIFLILCMASSGLSLSTNLIILSTSFNVMNYGAVGDGSTDDSQ------------ 60

Query: 61  WPSDDRDRWIQFSDIDGLMVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREM 120
                                                   C  P AL  + CNGL+L  +
Sbjct: 61  -------------------------------------GAQCNVPAALSFNRCNGLRLSGL 120

Query: 121 KHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIA 180
            HINS R+HI+I   N AIIS+L I+APE SPNTDGIDIS S+ V I+N  +GTGDDCIA
Sbjct: 121 THINSPRSHITITKSNGAIISNLRIIAPETSPNTDGIDISSSSQVQIRNCIIGTGDDCIA 180

Query: 181 LNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNG 240
           +  GS+NINI GVTCGPGHGISIG+LG+ G Y+ VE+IHV+NC  KGT NGVRIKTWQ G
Sbjct: 181 IGGGSSNINITGVTCGPGHGISIGALGQAG-YDRVEEIHVRNCTFKGTMNGVRIKTWQGG 240

Query: 241 YGYARGITFEQITLIDVKNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENA 300
            GYAR I+FE+I  I   NPI IDQ+Y       +N+   IK+SD++Y  + GTS  +N 
Sbjct: 241 NGYARKISFEKIKFIAAGNPIFIDQFYCPSQVNCQNKTSAIKLSDISYTGIIGTSTVDNV 300

Query: 301 IILNCSRS-RCSNIIMENVSIKNIDPGKEAKILCQNVDGR-------------------- 360
           I L+CS+S  C+NI++++V I +  PG      C +  GR                    
Sbjct: 301 INLSCSQSVGCTNIVLDHVYITSTTPGNRVYANCFDAHGRASHTRPADYLTVFLILCMAS 360

Query: 361 -----STSAIPTSPSFNVLDYGAIGNGQADDSQLKGTLVAP-KKGSWPKYDRERWIQFSD 420
                +T+ + ++ SF+V++YGA+G+G  DDSQ+ G +VAP +K +W  Y   RW+ FS+
Sbjct: 361 SGLSTTTNLVSSTSSFDVMNYGAVGDGTTDDSQVSGNIVAPNEKSAWTGYHINRWLTFSN 420

Query: 421 IDGLIVNGTGQFDGQGSSWW-----EDWEH--------CQRPTALFLHNCNGLQLREMKH 480
           I+GLI+NG GQ DG+GS+WW     E+ ++        C+ PTAL  + CNGL+L  + H
Sbjct: 421 INGLIINGRGQIDGKGSAWWPQPCLENVQYDTICKGPTCKGPTALTFNRCNGLRLDGLTH 480

Query: 481 INSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALN 540
           INS ++HI +  CN  IIS+LHIIAPE SPNTDGID+  S N+ I+N  +GTGDDCIA+ 
Sbjct: 481 INSPRSHIVLTNCNSSIISNLHIIAPETSPNTDGIDIYGSTNIQIRNCNIGTGDDCIAIC 540

Query: 541 NGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYG 600
            GSSN+DI GV CGPGHGISIG+L G+  Y+ VENI V+NC L+GT NG+RIK+WQGG G
Sbjct: 541 GGSSNVDIAGVICGPGHGISIGAL-GHGGYDAVENIKVRNCTLRGTTNGVRIKSWQGGNG 600

Query: 601 YARNITFEQITLMGAKNPIIIDQYYTGLAKAQKNKLFDIKVSNVTYREVNGTSANENAII 660
           YAR I+FE+I  + A NPIIIDQ+Y       +NK   IK+S+++Y  ++GTS ++  I 
Sbjct: 601 YARKISFEKINFIAASNPIIIDQFYCPPGVDCQNKTSAIKLSDISYTAISGTSTSDKVID 658

Query: 661 LNCSRN-RCSNIILENVSIKPIDPKMEAKAFCQNVDGRSTYAVPTVPCL 665
           L+CS++  C+NI+L++V I P        A C N  GR+++  P V CL
Sbjct: 661 LSCSQSVGCTNIVLDHVYITPTVQGKRIYANCFNAHGRASHTKPAVKCL 658

BLAST of Moc10g03010 vs. ExPASy TrEMBL
Match: A0A7J6WH92 (Polygalacturonase adpg1 OS=Thalictrum thalictroides OX=46969 GN=FRX31_013607 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 2.7e-166
Identity = 326/770 (42.34%), Postives = 443/770 (57.53%), Query Frame = 0

Query: 6    ALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQ--------------------- 65
            ++  I S   L   +  +  + +VLD+GA+G+G TDDSQ                     
Sbjct: 543  SVFIIISTIFLFLCLAPSGMSSSVLDFGAVGDGNTDDSQAFLKAWQTICESAEVRPTLII 602

Query: 66   -------------------------LGGTVVAPTKDSWP-SDDRDRWIQFSDIDGLMVNG 125
                                     + G +VAP   SWP + D + W++F+D+ GL+V G
Sbjct: 603  PAGKTFFLNPVSFEGPCKPANIRVWILGNLVAPVMSSWPKTADVNEWLKFADVSGLIVAG 662

Query: 126  SGQFDGQGSSWWKDCDKH--------CTRPTALFLHNCNGLQLREMKHINSGRNHISINA 185
             GQ +G+G +WW DC +         C RPTAL  ++C  L+L ++  INS  NHISIN 
Sbjct: 663  RGQINGRGETWW-DCGRKISLCKLDGCNRPTALKFNDCTDLKLSDITSINSQSNHISINE 722

Query: 186  CNDAIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVT 245
            C  A I+++H++APEDS NTDGIDIS SNNV+IQ+SF+ TGDDC+A+N GS+ INI    
Sbjct: 723  CKGATITNVHLIAPEDSNNTDGIDISKSNNVVIQDSFIATGDDCVAINGGSSFINITNTA 782

Query: 246  CGPGHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITL 305
            CGPGHGIS+GSLGENG  ++VE IHVKNC   GT NGVRIKTW  G GYA+ +TFEQI  
Sbjct: 783  CGPGHGISVGSLGENGDSSIVEGIHVKNCSFTGTTNGVRIKTWPFGTGYAKKLTFEQIRF 842

Query: 306  IDVKNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRS-RCSNI 365
             +VKNPIIIDQYY         +   I+VSDVTY  VHGTS    AI LNCS +  C+NI
Sbjct: 843  ENVKNPIIIDQYYCPDDNC-GGKSSAIQVSDVTYIGVHGTSVMPTAITLNCSATVPCNNI 902

Query: 366  IMENVSIKNIDPGKEAKILCQNVDGRSTSAIPT------------------------SPS 425
            +M +V+IK+ + G     +C N  G+ +   PT                        + +
Sbjct: 903  VMNDVNIKSTE-GIPTTAICNNAHGKESLTDPTEFWLIFMFLCLFPFSWSSDLDAMITNN 962

Query: 426  FNVLDYGAIGNGQADDSQ------------------------------------------ 485
            FNV+ YGA+G+G+ DDSQ                                          
Sbjct: 963  FNVISYGAVGDGKTDDSQAFLNAWKAMCFAKFGIVFLTIPPSKTFLSRPLNFRGPCKSPI 1022

Query: 486  ----LKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWW----EDWEHC 545
                ++G ++AP+   W   D+ RW+ FSD++GLIV G+G+ DG G SWW    +    C
Sbjct: 1023 VKVSVEGNIIAPEMSKWNNGDKSRWLTFSDVNGLIVTGSGRIDGNGQSWWVCAGKSGSGC 1082

Query: 546  QRPTALFLHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSR 605
             RP AL  H+CN L +  +  +NS KNHI+IN C    IS++HII+P+ SPNTDGI++S 
Sbjct: 1083 SRPAALTFHDCNNLDVSGLNIVNSPKNHITINDCKGAKISNIHIISPDSSPNTDGINLSN 1142

Query: 606  SNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVK 645
              NV I++S + +GDDCIA+N+G S ++I  V CGPGHGIS+GSLG N ++  VE IHV+
Sbjct: 1143 VMNVQIRDSIIKSGDDCIAINDGCSFVNITNVGCGPGHGISVGSLGANGDHCAVEEIHVR 1202

BLAST of Moc10g03010 vs. ExPASy TrEMBL
Match: A0A2H5QFV3 (Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_222910 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-155
Identity = 310/766 (40.47%), Postives = 422/766 (55.09%), Query Frame = 0

Query: 10  IFSIHLLSSWVVS-TSPNFNVLDYGAIGNGQTDDS------------------------- 69
           ++ ++L+S+  +   +  FNVLD+GA G+G  DDS                         
Sbjct: 8   LYFLYLISTASIRINADEFNVLDFGAAGDGNADDSMAFIKAWNNTCSATAKSPTMVIPKG 67

Query: 70  ---------------------QLGGTVVAPT-KDSWPSDDRDRWIQFSDIDGLMVNGSGQ 129
                                 L G+++AP   + W  DD+ +W +   +D L ++GSG 
Sbjct: 68  KTFLVHPITFTGPCKSSDITIMLSGSIIAPNGPNEWKEDDKSKWFEIQGVDSLQIDGSGT 127

Query: 130 FDGQGSSWW---------KDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACN 189
            DG+G +WW         + C K    PT L    CN L++  M  INS + H+++  CN
Sbjct: 128 IDGRGKAWWDISCKINKKEGCIK--LAPTVLKFDGCNNLRMNNMNFINSPQTHVTLMGCN 187

Query: 190 DAIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCG 249
                 L I AP  SPNTDGI I  S+NV I N+ +  GDDCI++ + ++NINI  V CG
Sbjct: 188 GVEFGFLVIQAPGTSPNTDGIHIQASSNVYIHNAHISNGDDCISIGDNTSNINITQVDCG 247

Query: 250 PGHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLID 309
           PGHG+SIGSLG++G    VE+I V N   KGT NG RIKTWQ G G  + I F  +   D
Sbjct: 248 PGHGVSIGSLGKDGGEVHVENITVSNINFKGTTNGARIKTWQVGKGTVKHIVFSNLNFTD 307

Query: 310 VKNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSR-CSNIIM 369
           V+NPIIIDQ+Y D+A + K     + + DV Y +  GTS  E AI LNCS S  C+NII+
Sbjct: 308 VENPIIIDQHYCDVADSCKETDTGVHIDDVLYAQSFGTSKTEVAINLNCSSSSPCTNIIV 367

Query: 370 ENVSIKNIDPGKE-----AKILCQNVDGRSTSAIPTSPSFNVLDYGAIGNGQADDS---- 429
           + + +K  +PG+        +L +      + + P +  +NV+++GA+GNG  DDS    
Sbjct: 368 DTIQLKPANPGQRELFFIISLLMRMATLGWSRSYPGAMPYNVMNFGAVGNGVTDDSQAFI 427

Query: 430 ------------------------------------------QLKGTLVAPK-KGSWPKY 489
                                                     Q++G L+AP+   SW   
Sbjct: 428 KAWNAVCGDTSNNPTLQVPQGKTFLLQPTSFQGPCKSSNLQVQIEGNLIAPEGPSSWKGK 487

Query: 490 DRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALFLHNCNGLQLREMKHINS 549
           DR  W+ F++++G  VNG G+ DGQGS W   W  C    AL    C G Q+  +KHINS
Sbjct: 488 DRRSWLYFANVNGFTVNGNGEIDGQGSQW---WNLCS--DALVFEKCTGFQVSGLKHINS 547

Query: 550 AKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGS 609
            KNHIS++ C D  +S+LHI APE SPNTDGID+S S N+ I NS + TGDDC+A+N GS
Sbjct: 548 QKNHISLDNCQDATLSNLHISAPESSPNTDGIDISASQNIHILNSNIATGDDCVAINTGS 607

Query: 610 SNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYAR 665
           S I++ G+TCGPGHGISIGSLG   E   VE +HVKNC L  TQNG+RIKTWQGG GYAR
Sbjct: 608 SQINVTGLTCGPGHGISIGSLGKQGEAAAVEEVHVKNCTLNATQNGLRIKTWQGGSGYAR 667

BLAST of Moc10g03010 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 3.5e-81
Identity = 159/307 (51.79%), Postives = 202/307 (65.80%), Query Frame = 0

Query: 363 QLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALF 422
           Q+ G LVAP KG+W K D+++WI F+DI+GL++ G G+ +GQGSSWWE      RPTAL 
Sbjct: 86  QMLGKLVAPSKGNW-KGDKDQWILFTDIEGLVIEGDGEINGQGSSWWE--HKGSRPTALK 145

Query: 423 LHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQ 482
             +CN L+L  + H++S   HI I+ CN V IS L I APE SPNTDGIDV  S+NV+IQ
Sbjct: 146 FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQ 205

Query: 483 NSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGT 542
           +  + TGDDCIA+N+G+SNI I G+ CGPGHGISIGSLG + E   VEN+ V+NC  +GT
Sbjct: 206 DCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGT 265

Query: 543 QNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG--LAKAQKNKLFDIKVSNV 602
            NG RIKTWQGG GYAR ITF  ITL   +NPIIIDQ+Y G     A+  K   ++VS V
Sbjct: 266 MNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKV 325

Query: 603 TYREVNGTSANENAIILNCS-RNRCSNIILENVSIKPID--PKMEAKAFCQNVDGRSTYA 662
            +    GTS +E  +   CS R  C+ I L ++ I+         A+  C NV G ST A
Sbjct: 326 VFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIA 385

Query: 663 VPTVPCL 665
           VP + CL
Sbjct: 386 VPGLECL 389

BLAST of Moc10g03010 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 9.5e-79
Identity = 157/307 (51.14%), Postives = 200/307 (65.15%), Query Frame = 0

Query: 363 QLKGTLVAPKKGSWPKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALF 422
           Q+ G LVAP KG+W K D+++WI F+DI+GL++ G G+ +GQGSSWWE      RPT   
Sbjct: 85  QMLGKLVAPSKGNW-KGDKDQWILFTDIEGLVIEGDGEINGQGSSWWE--HKGSRPT--- 144

Query: 423 LHNCNGLQLREMKHINSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQ 482
             +CN L+L  + H++S   HI I+ CN V IS L I APE SPNTDGIDV  S+NV+IQ
Sbjct: 145 FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQ 204

Query: 483 NSFMGTGDDCIALNNGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGT 542
           +  + TGDDCIA+N+G+SNI I G+ CGPGHGISIGSLG + E   VEN+ V+NC  +GT
Sbjct: 205 DCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGT 264

Query: 543 QNGMRIKTWQGGYGYARNITFEQITLMGAKNPIIIDQYYTG--LAKAQKNKLFDIKVSNV 602
            NG RIKTWQGG GYAR ITF  ITL   +NPIIIDQ+Y G     A+  K   ++VS V
Sbjct: 265 MNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKV 324

Query: 603 TYREVNGTSANENAIILNCS-RNRCSNIILENVSIKPID--PKMEAKAFCQNVDGRSTYA 662
            +    GTS +E  +   CS R  C+ I L ++ I+         A+  C NV G ST A
Sbjct: 325 VFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIA 384

Query: 663 VPTVPCL 665
           VP + CL
Sbjct: 385 VPGLECL 385

BLAST of Moc10g03010 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 285.4 bits (729), Expect = 1.2e-76
Identity = 156/360 (43.33%), Postives = 212/360 (58.89%), Query Frame = 0

Query: 26  NFNVLDYGAIGNGQTDDS------------------------------------------ 85
           N+NVL++ A G+GQTDDS                                          
Sbjct: 23  NYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQGPCKSS 82

Query: 86  ----QLGGTVVAPT-KDSWPSDDRDRWIQFSDIDGLMVNGSGQFDGQGSSWWKDCDKHCT 145
               QL GT+VAP+ K +W       WI+FS + GL++ GSG  D +GSS+W+   K   
Sbjct: 83  SIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELNLKASQ 142

Query: 146 RPTALFLHNCNGLQLREMKHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGIDISGS 205
           RPTAL +  C+ L++  +  I+S +NHISI  CN   IS++++ APE SPNTDGIDIS S
Sbjct: 143 RPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGIDISDS 202

Query: 206 NNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVEDIHVKN 265
            N+ I +S + TGDDCIA+N+GS+NINI G+ CGPGHGIS+GSLG  G    V D+ V +
Sbjct: 203 TNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSDVQVTH 262

Query: 266 CLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVKNPIIIDQYYSDLAKAQKNRLLPIK 325
           C    T NG RIKTW  G GYAR I+F  ITL++ KNPIIIDQ+Y D  K +      + 
Sbjct: 263 CTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHYID--KGRLTEESAVA 322

Query: 326 VSDVTYREVHGTSANENAIILNCSR-SRCSNIIMENVSIKNIDPGKEAKILCQNVDGRST 338
           +S+V + +  GTS+N+NAI L CS  + C +++M+ + I   + GK  K+ CQ VDG S+
Sbjct: 323 ISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMANGGK-PKVNCQYVDGESS 379

BLAST of Moc10g03010 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 7.0e-74
Identity = 151/359 (42.06%), Postives = 212/359 (59.05%), Query Frame = 0

Query: 27  FNVLDYGAIGNGQTDDS------------------------------------------- 86
           +NVL +GA G+GQTDDS                                           
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  ---QLGGTVVAPTK-DSWPSDDRDRWIQFSDIDGLMVNGSGQFDGQGSSWWKDCDKHCTR 146
              QL G +VAP+   SW +     WI FS + GLM++GSG  +G+GSS+W+        
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWE-------- 172

Query: 147 PTALFLHNCNGLQLREMKHINSGRNHISINACNDAIISDLHIMAPEDSPNTDGIDISGSN 206
             AL + NCN L +  +  I+S ++HISI  C+   IS ++I+APE+SPNTDGIDIS S 
Sbjct: 173 --ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYST 232

Query: 207 NVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPGHGISIGSLGENGQYNVVEDIHVKNC 266
           NV I +S + TGDDCIA+N GS++INI  V CGPGHGIS+GSLG +G+   V D++V  C
Sbjct: 233 NVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQC 292

Query: 267 LLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVKNPIIIDQYYSDLAKAQKNRLLPIKV 326
               T NG RIKTWQ G GYAR I+FE ITLI+V+NPIIIDQ Y+D       +   + +
Sbjct: 293 TFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAI 352

Query: 327 SDVTYREVHGTSANENAIILNCSR-SRCSNIIMENVSIKNIDPGKEAKILCQNVDGRST 338
           S V Y    GT+ NE+AI+L CS  + C +++++++ +  ++ G++ K+ C+NV+G S+
Sbjct: 353 SSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEV-TMENGEKPKVECENVEGESS 400

BLAST of Moc10g03010 vs. TAIR 10
Match: AT4G32375.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 269.6 bits (688), Expect = 6.6e-72
Identity = 147/351 (41.88%), Postives = 208/351 (59.26%), Query Frame = 0

Query: 323 KEA-KILCQ-NVDGRSTSAIPTSPSFNVLDYGAIGNGQADDS---QLKGTLVAPKK-GSW 382
           KEA   LC+ NV+G +TS +  +    ++         A  +   Q+ G L APK    W
Sbjct: 7   KEAWNALCEANVNGTTTSLVINANETYIVQPQLFQGPCASRNIHIQIDGKLEAPKMVREW 66

Query: 383 PKYDRERWIQFSDIDGLIVNGTGQFDGQGSSWWEDWEHCQRPTALFLHNCNGLQLREMKH 442
             Y+ + W+ F+++ GL++NG+G     G +WW   EH  RP  +     + +    +  
Sbjct: 67  GNYESKCWLCFTNVKGLVLNGSGILHPHGEAWWSSIEHSHRPRTIGFSGSSNILYNGLTQ 126

Query: 443 INSAKNHISINACNDVIISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALN 502
           +NS KNHISI  C +V +S+ H+IAP+DSPNTDGID++ SNN+ I NS + TGDDCIA+N
Sbjct: 127 MNSPKNHISILDCTNVTLSNFHLIAPKDSPNTDGIDIAHSNNIRIFNSSIQTGDDCIAIN 186

Query: 503 NGSSNIDIVGVTCGPGHGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTW--QGG 562
            GS +I+I  V CGPGHGISIGSLG     + V+N+ +++C   GT+NG RIKTW  +GG
Sbjct: 187 GGSYDINITHVACGPGHGISIGSLGRYSVNDTVQNVKIRHCSFNGTENGARIKTWTVRGG 246

Query: 563 YGYARNITFEQITLMGAKNPIIIDQYY-TGLAKAQKNKLFDIKVSNVTYREVNGTSANEN 622
            G A+NI +E ITL   K PIIIDQ+Y  G     K  +  +KVSNVT+R   GT AN+ 
Sbjct: 247 LGVAKNILYENITLTDTKYPIIIDQHYCNGGHNCTKEAMTAVKVSNVTFRYFTGTCANDI 306

Query: 623 AIILNCSR-NRCSNIILENVSIKPIDPKMEAKAFCQNVDGRSTYAVPTVPC 664
           AI L+C     C +I++E+++I     K    A+CQ  D  S +    + C
Sbjct: 307 AIKLDCDEVTGCKDIVMEHINITSSSTKRPLTAYCQFADIISHFVSMKIKC 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141108.11.5e-19087.18probable polygalacturonase At3g15720 [Momordica charantia][more]
XP_022141107.13.5e-17683.82probable polygalacturonase At3g15720 [Momordica charantia][more]
KAA8540480.12.3e-16745.98hypothetical protein F0562_024601 [Nyssa sinensis][more]
KAF5196806.15.6e-16642.34Polygalacturonase adpg1 [Thalictrum thalictroides][more]
XP_023920973.19.3e-16142.54uncharacterized protein LOC112032446 [Quercus suber][more]
Match NameE-valueIdentityDescription
Q9LW074.9e-8051.79Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q94AJ55.5e-6339.94Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
Q8RY296.0e-6244.19Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Q020966.2e-5939.87Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Q9FY191.4e-5840.31Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CJJ67.1e-19187.18probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1CIX81.7e-17683.82probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A5J5BDR41.1e-16745.98Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_024601 PE=3 SV=1[more]
A0A7J6WH922.7e-16642.34Polygalacturonase adpg1 OS=Thalictrum thalictroides OX=46969 GN=FRX31_013607 PE=... [more]
A0A2H5QFV31.3e-15540.47Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_222910 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G15720.13.5e-8151.79Pectin lyase-like superfamily protein [more]
AT3G15720.29.5e-7951.14Pectin lyase-like superfamily protein [more]
AT4G35670.11.2e-7643.33Pectin lyase-like superfamily protein [more]
AT5G27530.17.0e-7442.06Pectin lyase-like superfamily protein [more]
AT4G32375.16.6e-7241.88Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 499..519
e-value: 6600.0
score: 1.4
coord: 181..201
e-value: 8100.0
score: 0.7
coord: 558..579
e-value: 3300.0
score: 3.5
coord: 211..232
e-value: 29.0
score: 13.5
coord: 449..475
e-value: 110.0
score: 11.7
coord: 131..157
e-value: 170.0
score: 11.0
coord: 158..179
e-value: 270.0
score: 10.3
coord: 476..497
e-value: 270.0
score: 10.3
coord: 529..550
e-value: 89.0
score: 11.9
coord: 240..261
e-value: 4600.0
score: 2.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 344..654
e-value: 6.5E-91
score: 306.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 17..343
e-value: 5.6E-94
score: 317.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 364..651
e-value: 5.3E-82
score: 275.5
coord: 45..333
e-value: 1.2E-82
score: 277.6
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 506..519
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 188..201
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 362..664
NoneNo IPR availablePANTHERPTHR31375:SF232SUBFAMILY NOT NAMEDcoord: 362..664
coord: 44..343
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 44..343
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 338..664
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 12..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g03010.1Moc10g03010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity