Moc10g02900 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g02900
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Mediator of RNA polymerase II transcription subunit 31
Location	chr10: 1998267 .. 2001057 (-)
RNA-Seq Expression	Moc10g02900
Synteny	Moc10g02900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCTAATCATGGATTAAACGAAGAAGCATCTGATAACCCATCATCGTAAGTGTGTTTCTCTGTTCTTTCTCGTGTACTGACTTTTTCCTCGTTGTTAATTTCTTTTTAGCCAATTCTTCTTTCGTTTTGGTTATAGCTCCAATTCGTCTTTTCCGTTTGGTTGGTGCGAAATGTAAACGGAAGAAAGTGATCTCTAGTTTATGTCAATGATTTTTAGCATAGTTATTATCTTCCACTGGACCGATTGATTTACAGCACTGCTTGTCAATTGATCGATTTTCCCTTTCTGTCATTTTATTTTCTGTTTGGTGAATAATTGCCAGGCCTACGAATGTATACAAGGATCCAGATGACGGGCGGCAGCGGTTCTTGCTCGAATTGGAATTTGTTCAATGTCTCGCCAATCCAACCTACATTCACTGTACGTTGTTGCAAAATTTAGGGTTCTATATTGGCAGTTGGATAATTTCTAATGCTTGAAACTTCAAATTGAAATGAGTATGTGATTGTATACTAACCCATTTAAGATAAAACGGTCCACTATTTTTTTTTTTGTTGTAGTTAAAGAATTACTTCCAAACCCGAACCCTTTCTAGATGAATTCAGTTTCACTTGGGATTTCTACTGTGCTAAACAGACATTACATTGTGCTTGACATAAAAACGAACCAATCTGTAAAGCAAATTCTACTTCTTTTTTCTAATGACAAAGTAGATTCATCTGTTAGCTTAAATTTTCCTATTGTAATACCTCATAGGAATTTTGTTTTTGTTTATTCATAATTTTCGCAGTTGCATTTCCCTGTGAAGTGTTAATTTTAAATTTATCAGTGCGAAAAAGAAAAATACTTTGAGATATCAGATTTTGGGCACTCAGAACCTGTGTTCCTTCCAAATTTGCAGCTTCATGAACTAGTGTAGCACCTGGATAAAATTTTATCTTTTATGTGCCTGTATCTTACTGTTTTTACTACAAATTCACCATAGAATTTATCTTTTATGTGCCTTCTCAACTCAGAGATTGGTGCTCAATCTTTTTGCCTTAGCAGTGTACCACTCCATACAGTGGTTCCACATATAATGTATTGCCCGGAATTCTATTCTTTTGATGATATGTTATGGTCTCGTACGACTAAAGTACATTTCTGAAGTATCACCCTTTTCTTAATTTTCTTGAGGCAGTTATCGTCACGTACTCACTTGAATATACTCCTGCATATGCAAATTGGCATTGAAATATTGGAACAAATGCTCTTTATCCTCCCATTTCTGATAGCTGATCATCAAGTTTTTATACAGATCTGGCTCAGAATCGTTACTTTGAGGATGAAGCTTTCATTGGTTACTTGAAGTACCTTCAATATTGGCAACGACCAGAGTATATTAAGTTTATAATGTGAGTAAGATCTTTCCATCTTGTGTTACACGAGCTTTTCCCTAAACTGAAGACACTATTGGGATTGACTTTAATGGTTTTTCTAGGTATCCTCATAGTTTGTTTTTCCTTGAACTTCTACAAAATGCAAACTTCCGAAATGCAATGGCTCATCCTGCCAACAAGGTTAATGGAATCTCTATGACCTTTTCTTTCTGCTCGATTTCCTTCTTGATATTAGATAATCAATATTAATTTGATTGTAAATTTTTTTGGCTCCCTTGTCCCTGGTACGATTTTATCAAGTACCAGTTCAATTGTTATCAGGCTTCTCTTTCCTCATCACAGTAACCACCTGGTTCTTCTAATATTGGTTTTAAACTAGCTAATATTTTAAGGGAAAGTGGTGCAACATTGAGGTTGTTCATTGGTCCTTTTTTTTTCATGTATAATTATTTTAAAGAGATTTACTCTATTCGCTATCAGTTTTATGCTTTGATCTACTTCATCAATGTTTGGGACTGTAAGGAAACGTCTCTGGTATAGTTTGACTGTTATGTTTTATTTCTTGTTTGGCATGACTGGTCAAATTCGAAACGTCTCCGATCTCCACCAGTTTGTGATAGATATTTGTTGCTTACTTGAACTGTGTGAGAGTCCTATTAGGGATAGTTTAGGAATATTATTAGTATGGTAGTGGGGGCCTTATGGTAATTAGTTAGGGGTGAGTGGAATATAATAGAGGTGGTGGGAGAGAAGGAGGTCATCTGTTGGGAATAGTCTAAGGTGGAGAACTTAGTATAGTTCTTGGGAGAGAGATATCCCTCTTGAAAGGCTATCGATATTGTAGCATCTTTTTGAGATTGCAATACCGTTATCTTGTTTCGTTTAGTGTATTTCCCTAACAAACTGTTTACTCTTTATGTCAGATATTTTTCCTTTTCCCTTTTCTTAACATTTTAACACACTTTCCACTATTTGGAATTTCTTAGGAATTGTCACACAGGCAGCAATTTTACTTTTGGAAGAACTACAGAAACAATCGTTTGAAACACATTTTACCGAGACCGCTTCCCGAACCTGCAGCTTTACCACCCCCAGTTTCTGTTCCACCTCAGGCACCTGTGCCCGCTGCAACAGCCCCTGGCATGGCAGCTTCTCCTGTTGGTGCTCCTGCCCTTTCTCCAATGCAATATGGTATTCCCCCTGGGTCTGGACTTGCAAAAAATGACATGAAGAGTGGTGGAATTGATCGACGAAAGAGAAAACAACACGTAATTTATCGCATATCATCTGTTTTTTTGTCTCTTTAAACTGATCAGTGCTCTCAATGAGTAGGTCTAGAAGCAGTTTGACCATTCAAATTCTAATTCATTTCTTTTGTATTTTGACAGGAAAGAAGTATGACTTAG

mRNA sequence

ATGGCCTCTAATCATGGATTAAACGAAGAAGCATCTGATAACCCATCATCGCCTACGAATGTATACAAGGATCCAGATGACGGGCGGCAGCGGTTCTTGCTCGAATTGGAATTTGTTCAATGTCTCGCCAATCCAACCTACATTCACTATCTGGCTCAGAATCGTTACTTTGAGGATGAAGCTTTCATTGGTTACTTGAAGTACCTTCAATATTGGCAACGACCAGAGTATATTAAGTTTATAATGTATCCTCATAGTTTGTTTTTCCTTGAACTTCTACAAAATGCAAACTTCCGAAATGCAATGGCTCATCCTGCCAACAAGGAATTGTCACACAGGCAGCAATTTTACTTTTGGAAGAACTACAGAAACAATCGTTTGAAACACATTTTACCGAGACCGCTTCCCGAACCTGCAGCTTTACCACCCCCAGTTTCTGTTCCACCTCAGGCACCTGTGCCCGCTGCAACAGCCCCTGGCATGGCAGCTTCTCCTGTTGGTGCTCCTGCCCTTTCTCCAATGCAATATGGTATTCCCCCTGGGTCTGGACTTGCAAAAAATGACATGAAGAGTGGTGGAATTGATCGACGAAAGAGAAAACAACACGAAAGAAGTATGACTTAG

Coding sequence (CDS)

ATGGCCTCTAATCATGGATTAAACGAAGAAGCATCTGATAACCCATCATCGCCTACGAATGTATACAAGGATCCAGATGACGGGCGGCAGCGGTTCTTGCTCGAATTGGAATTTGTTCAATGTCTCGCCAATCCAACCTACATTCACTATCTGGCTCAGAATCGTTACTTTGAGGATGAAGCTTTCATTGGTTACTTGAAGTACCTTCAATATTGGCAACGACCAGAGTATATTAAGTTTATAATGTATCCTCATAGTTTGTTTTTCCTTGAACTTCTACAAAATGCAAACTTCCGAAATGCAATGGCTCATCCTGCCAACAAGGAATTGTCACACAGGCAGCAATTTTACTTTTGGAAGAACTACAGAAACAATCGTTTGAAACACATTTTACCGAGACCGCTTCCCGAACCTGCAGCTTTACCACCCCCAGTTTCTGTTCCACCTCAGGCACCTGTGCCCGCTGCAACAGCCCCTGGCATGGCAGCTTCTCCTGTTGGTGCTCCTGCCCTTTCTCCAATGCAATATGGTATTCCCCCTGGGTCTGGACTTGCAAAAAATGACATGAAGAGTGGTGGAATTGATCGACGAAAGAGAAAACAACACGAAAGAAGTATGACTTAG

Protein sequence

MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWKNYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPPGSGLAKNDMKSGGIDRRKRKQHERSMT

Homology

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BLAST of Moc10g02900 vs. NCBI nr
Match: XP_022141008.1 (mediator of RNA polymerase II transcription subunit 31 [Momordica charantia])

HSP 1 Score: 415.6 bits (1067), Expect = 2.5e-112
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE
Sbjct: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180

Query: 181 GSGLAKNDMKSGGIDRRKRKQHERSMT 208
           GSGLAKNDMKSGGIDRRKRKQHERSMT
Sbjct: 181 GSGLAKNDMKSGGIDRRKRKQHERSMT 207

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BLAST of Moc10g02900 vs. NCBI nr
Match: XP_038880162.1 (mediator of RNA polymerase II transcription subunit 31 [Benincasa hispida])

HSP 1 Score: 370.2 bits (949), Expect = 1.2e-98
Identity = 188/206 (91.26%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASN GLNEEA+ NPSSPT+VYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNPGLNEEAAGNPSSPTSVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH LFFLELLQN+NFRNAMAHP NKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEPAALPPPVS PPQAPVP A AP MAASP G PALSPMQYGIPP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSAPPQAPVP-APAPAMAASPAGTPALSPMQYGIPP 180

Query: 181 GSGLAKNDMKSGGIDRRKRKQHERSM 207
           GSGL KNDMKS GIDRRKRK HERSM
Sbjct: 181 GSGLPKNDMKSAGIDRRKRK-HERSM 204

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BLAST of Moc10g02900 vs. NCBI nr
Match: XP_008453845.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis melo] >KAA0044690.1 mediator of RNA polymerase II transcription subunit 31 [Cucumis melo var. makuwa] >TYK16894.1 mediator of RNA polymerase II transcription subunit 31 [Cucumis melo var. makuwa])

HSP 1 Score: 368.2 bits (944), Expect = 4.5e-98
Identity = 189/208 (90.87%), Postives = 193/208 (92.79%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGLNEEA+DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNHGLNEEAADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH LFFLELLQN+NFRNAMAHP NKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAAT-APGMAASPVGAPALSPMQYGIP 180
           NYRNNRLKHILPRPLPEPAALPPPVS PPQAPVPA T AP +AASP  A ALSPMQYGIP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSAPPQAPVPAPTPAPTVAASPATA-ALSPMQYGIP 180

Query: 181 PGSGLAKNDMKSGGIDRRKRKQHERSMT 208
           PG GL KNDMK  GIDRRKRK HERSMT
Sbjct: 181 PGPGLPKNDMKGAGIDRRKRK-HERSMT 206

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BLAST of Moc10g02900 vs. NCBI nr
Match: XP_004146891.1 (mediator of RNA polymerase II transcription subunit 31 [Cucumis sativus] >KGN53186.1 hypothetical protein Csa_014598 [Cucumis sativus])

HSP 1 Score: 363.2 bits (931), Expect = 1.5e-96
Identity = 186/208 (89.42%), Postives = 191/208 (91.83%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGL EE +DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH LFFLELLQN+NFRNAMAHP NKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAAT-APGMAASPVGAPALSPMQYGIP 180
           NYRNNRLKHILPRPLPEPAALPPPV+ PPQA VPA T AP MAASP  APALSP+QYGIP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASP-AAPALSPIQYGIP 180

Query: 181 PGSGLAKNDMKSGGIDRRKRKQHERSMT 208
           PG GL KNDMK  GIDRRKRK HERSMT
Sbjct: 181 PGPGLPKNDMKGAGIDRRKRK-HERSMT 206

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BLAST of Moc10g02900 vs. NCBI nr
Match: XP_022990254.1 (mediator of RNA polymerase II transcription subunit 31-like [Cucurbita maxima] >XP_023535403.1 mediator of RNA polymerase II transcription subunit 31-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 9.5e-96
Identity = 182/206 (88.35%), Postives = 191/206 (92.72%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASN+GLNEEA+D+PSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNYGLNEEAADSPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQ+PEYIK+IMYPH LFFLELLQNANFR+AMAHPANKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQQPEYIKYIMYPHCLFFLELLQNANFRSAMAHPANKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180
           NYRNNRLKHILPR  PEPAALPPPVS P QAPVP  TAP MAASP   PALSPMQYGIPP
Sbjct: 121 NYRNNRLKHILPRSFPEPAALPPPVSAPSQAPVP-TTAPAMAASPAATPALSPMQYGIPP 180

Query: 181 GSGLAKNDMKSGGIDRRKRKQHERSM 207
           GSGL+KNDMK+  IDRRKRK HERSM
Sbjct: 181 GSGLSKNDMKNAAIDRRKRK-HERSM 204

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BLAST of Moc10g02900 vs. ExPASy Swiss-Prot
Match: Q8VYB1 (Mediator of RNA polymerase II transcription subunit 31 OS=Arabidopsis thaliana OX=3702 GN=MED31 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.8e-73
Identity = 148/206 (71.84%), Postives = 165/206 (80.10%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MAS   + ++AS+ PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRYFEDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH L+FLELLQN NFR AMAHPANKEL+HRQQFY+WK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKELAHRQQFYYWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATA--PGMAASPVGAPALSPMQYGI 180
           NYRNNRLKHILPRPLPEP        VPPQ PV  +T+  P  +A+   +PALSPMQY  
Sbjct: 121 NYRNNRLKHILPRPLPEP--------VPPQPPVAPSTSLPPAPSATAALSPALSPMQY-- 180

Query: 181 PPGSGLAKND---MKSGGIDRRKRKQ 202
              + L+KND   M + GIDRRKRK+
Sbjct: 181 --NNMLSKNDTRNMGATGIDRRKRKK 194

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BLAST of Moc10g02900 vs. ExPASy Swiss-Prot
Match: Q54NI7 (Putative mediator of RNA polymerase II transcription subunit 31 OS=Dictyostelium discoideum OX=44689 GN=med31 PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.0e-32
Identity = 59/97 (60.82%), Postives = 78/97 (80.41%), Query Frame = 0


Query: 31  RFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHSLFFL 90
           RF++ELEF+QCL+NP Y++YLAQNRYF+D+AF+ YL YLQYW++PEY KFI+YP SL+FL
Sbjct: 55  RFIMELEFIQCLSNPRYLNYLAQNRYFQDKAFVNYLVYLQYWKKPEYAKFIVYPQSLYFL 114

Query: 91  ELLQNANFRNAMAHPANKELSHRQQFYFWKNYRNNRL 128
           +LLQ   FR  + H  + +  H QQFY W+ YRNNR+
Sbjct: 115 DLLQEERFRQELNHSQSTDFIHEQQFYHWQYYRNNRM 151

[Close]

BLAST of Moc10g02900 vs. ExPASy Swiss-Prot
Match: A0JNN3 (Mediator of RNA polymerase II transcription subunit 31 OS=Bos taurus OX=9913 GN=MED31 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 51/105 (48.57%), Postives = 68/105 (64.76%), Query Frame = 0


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMY 83
           D    R RF LELEFVQCLANP Y+++LAQ  YF+D+AF+ YLKYL YW+ PEY K++ Y
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKEPEYAKYLKY 69

Query: 84  PHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWKNYRNNRLK 129
           P  L  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  PQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMR 114

[Close]

BLAST of Moc10g02900 vs. ExPASy Swiss-Prot
Match: Q9CXU1 (Mediator of RNA polymerase II transcription subunit 31 OS=Mus musculus OX=10090 GN=Med31 PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 3.8e-23
Identity = 51/105 (48.57%), Postives = 68/105 (64.76%), Query Frame = 0


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMY 83
           D    R RF LELEFVQCLANP Y+++LAQ  YF+D+AF+ YLKYL YW+ PEY K++ Y
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKEPEYAKYLKY 69

Query: 84  PHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWKNYRNNRLK 129
           P  L  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  PQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRVR 114

[Close]

BLAST of Moc10g02900 vs. ExPASy Swiss-Prot
Match: Q9Y3C7 (Mediator of RNA polymerase II transcription subunit 31 OS=Homo sapiens OX=9606 GN=MED31 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.0e-23
Identity = 51/105 (48.57%), Postives = 68/105 (64.76%), Query Frame = 0


Query: 24  DPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMY 83
           D    R RF LELEFVQCLANP Y+++LAQ  YF+D+AF+ YLKYL YW+ PEY K++ Y
Sbjct: 10  DDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKY 69

Query: 84  PHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWKNYRNNRLK 129
           P  L  LELLQ  +FR  + +    +    QQ   W++Y   R++
Sbjct: 70  PQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMR 114

[Close]

BLAST of Moc10g02900 vs. ExPASy TrEMBL
Match: A0A6J1CJB2 (Mediator of RNA polymerase II transcription subunit 31 OS=Momordica charantia OX=3673 GN=LOC111011520 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.2e-112
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE
Sbjct: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180
           NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180

Query: 181 GSGLAKNDMKSGGIDRRKRKQHERSMT 208
           GSGLAKNDMKSGGIDRRKRKQHERSMT
Sbjct: 181 GSGLAKNDMKSGGIDRRKRKQHERSMT 207

[Close]

BLAST of Moc10g02900 vs. ExPASy TrEMBL
Match: A0A5D3D0Y1 (Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00280 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.2e-98
Identity = 189/208 (90.87%), Postives = 193/208 (92.79%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGLNEEA+DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNHGLNEEAADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH LFFLELLQN+NFRNAMAHP NKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAAT-APGMAASPVGAPALSPMQYGIP 180
           NYRNNRLKHILPRPLPEPAALPPPVS PPQAPVPA T AP +AASP  A ALSPMQYGIP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSAPPQAPVPAPTPAPTVAASPATA-ALSPMQYGIP 180

Query: 181 PGSGLAKNDMKSGGIDRRKRKQHERSMT 208
           PG GL KNDMK  GIDRRKRK HERSMT
Sbjct: 181 PGPGLPKNDMKGAGIDRRKRK-HERSMT 206

[Close]

BLAST of Moc10g02900 vs. ExPASy TrEMBL
Match: A0A1S3BY12 (Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis melo OX=3656 GN=LOC103494449 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.2e-98
Identity = 189/208 (90.87%), Postives = 193/208 (92.79%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGLNEEA+DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNHGLNEEAADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH LFFLELLQN+NFRNAMAHP NKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAAT-APGMAASPVGAPALSPMQYGIP 180
           NYRNNRLKHILPRPLPEPAALPPPVS PPQAPVPA T AP +AASP  A ALSPMQYGIP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVSAPPQAPVPAPTPAPTVAASPATA-ALSPMQYGIP 180

Query: 181 PGSGLAKNDMKSGGIDRRKRKQHERSMT 208
           PG GL KNDMK  GIDRRKRK HERSMT
Sbjct: 181 PGPGLPKNDMKGAGIDRRKRK-HERSMT 206

[Close]

BLAST of Moc10g02900 vs. ExPASy TrEMBL
Match: A0A0A0KZ88 (Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis sativus OX=3659 GN=Csa_4G025160 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.1e-97
Identity = 186/208 (89.42%), Postives = 191/208 (91.83%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASNHGL EE +DNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNHGLKEEEADNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH LFFLELLQN+NFRNAMAHP NKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLFFLELLQNSNFRNAMAHPGNKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAAT-APGMAASPVGAPALSPMQYGIP 180
           NYRNNRLKHILPRPLPEPAALPPPV+ PPQA VPA T AP MAASP  APALSP+QYGIP
Sbjct: 121 NYRNNRLKHILPRPLPEPAALPPPVTAPPQAAVPAPTSAPNMAASP-AAPALSPIQYGIP 180

Query: 181 PGSGLAKNDMKSGGIDRRKRKQHERSMT 208
           PG GL KNDMK  GIDRRKRK HERSMT
Sbjct: 181 PGPGLPKNDMKGAGIDRRKRK-HERSMT 206

[Close]

BLAST of Moc10g02900 vs. ExPASy TrEMBL
Match: A0A6J1JRK2 (Mediator of RNA polymerase II transcription subunit 31 OS=Cucurbita maxima OX=3661 GN=LOC111487170 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.6e-96
Identity = 182/206 (88.35%), Postives = 191/206 (92.72%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MASN+GLNEEA+D+PSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRY EDE
Sbjct: 1   MASNYGLNEEAADSPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYLEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQ+PEYIK+IMYPH LFFLELLQNANFR+AMAHPANKEL+HRQQFYFWK
Sbjct: 61  AFIGYLKYLQYWQQPEYIKYIMYPHCLFFLELLQNANFRSAMAHPANKELAHRQQFYFWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATAPGMAASPVGAPALSPMQYGIPP 180
           NYRNNRLKHILPR  PEPAALPPPVS P QAPVP  TAP MAASP   PALSPMQYGIPP
Sbjct: 121 NYRNNRLKHILPRSFPEPAALPPPVSAPSQAPVP-TTAPAMAASPAATPALSPMQYGIPP 180

Query: 181 GSGLAKNDMKSGGIDRRKRKQHERSM 207
           GSGL+KNDMK+  IDRRKRK HERSM
Sbjct: 181 GSGLSKNDMKNAAIDRRKRK-HERSM 204

[Close]

BLAST of Moc10g02900 vs. TAIR 10
Match: AT5G19910.1 (SOH1 family protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-74
Identity = 148/206 (71.84%), Postives = 165/206 (80.10%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MAS   + ++AS+ PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRYFEDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANKELSHRQQFYFWK 120
           AFIGYLKYLQYWQRPEYIKFIMYPH L+FLELLQN NFR AMAHPANKEL+HRQQFY+WK
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKELAHRQQFYYWK 120

Query: 121 NYRNNRLKHILPRPLPEPAALPPPVSVPPQAPVPAATA--PGMAASPVGAPALSPMQYGI 180
           NYRNNRLKHILPRPLPEP        VPPQ PV  +T+  P  +A+   +PALSPMQY  
Sbjct: 121 NYRNNRLKHILPRPLPEP--------VPPQPPVAPSTSLPPAPSATAALSPALSPMQY-- 180

Query: 181 PPGSGLAKND---MKSGGIDRRKRKQ 202
              + L+KND   M + GIDRRKRK+
Sbjct: 181 --NNMLSKNDTRNMGATGIDRRKRKK 194

[Close]

BLAST of Moc10g02900 vs. TAIR 10
Match: AT5G19910.2 (SOH1 family protein )

HSP 1 Score: 261.2 bits (666), Expect = 7.3e-70
Identity = 148/236 (62.71%), Postives = 165/236 (69.92%), Query Frame = 0


Query: 1   MASNHGLNEEASDNPSSPTNVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDE 60
           MAS   + ++AS+ PS P N YKDPD GRQRFLLELEF+QCLANPTYIHYLAQNRYFEDE
Sbjct: 1   MASPEEMGDDASEIPSPPKNTYKDPDGGRQRFLLELEFIQCLANPTYIHYLAQNRYFEDE 60

Query: 61  AFIGYLKYLQYWQRPEYIKFIMYPHSLFFLELLQNANFRNAMAHPANK------------ 120
           AFIGYLKYLQYWQRPEYIKFIMYPH L+FLELLQN NFR AMAHPANK            
Sbjct: 61  AFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNPNFRTAMAHPANKWFKMLGYWCFWN 120

Query: 121 ------------------ELSHRQQFYFWKNYRNNRLKHILPRPLPEPAALPPPVSVPPQ 180
                             EL+HRQQFY+WKNYRNNRLKHILPRPLPEP        VPPQ
Sbjct: 121 VFWGLVEFRVSLVQNLAYELAHRQQFYYWKNYRNNRLKHILPRPLPEP--------VPPQ 180

Query: 181 APVPAATA--PGMAASPVGAPALSPMQYGIPPGSGLAKND---MKSGGIDRRKRKQ 202
            PV  +T+  P  +A+   +PALSPMQY     + L+KND   M + GIDRRKRK+
Sbjct: 181 PPVAPSTSLPPAPSATAALSPALSPMQY----NNMLSKNDTRNMGATGIDRRKRKK 224

The following BLAST results are available for this feature:

BLAST of Moc10g02900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141008.1	2.5e-112	100.00	mediator of RNA polymerase II transcription subunit 31 [Momordica charantia]	[more]
XP_038880162.1	1.2e-98	91.26	mediator of RNA polymerase II transcription subunit 31 [Benincasa hispida]	[more]
XP_008453845.1	4.5e-98	90.87	PREDICTED: mediator of RNA polymerase II transcription subunit 31 [Cucumis melo]...	[more]
XP_004146891.1	1.5e-96	89.42	mediator of RNA polymerase II transcription subunit 31 [Cucumis sativus] >KGN531...	[more]
XP_022990254.1	9.5e-96	88.35	mediator of RNA polymerase II transcription subunit 31-like [Cucurbita maxima] >...	[more]

BLAST of Moc10g02900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYB1	1.8e-73	71.84	Mediator of RNA polymerase II transcription subunit 31 OS=Arabidopsis thaliana O...	[more]
Q54NI7	2.0e-32	60.82	Putative mediator of RNA polymerase II transcription subunit 31 OS=Dictyostelium...	[more]
A0JNN3	2.9e-23	48.57	Mediator of RNA polymerase II transcription subunit 31 OS=Bos taurus OX=9913 GN=...	[more]
Q9CXU1	3.8e-23	48.57	Mediator of RNA polymerase II transcription subunit 31 OS=Mus musculus OX=10090 ...	[more]
Q9Y3C7	5.0e-23	48.57	Mediator of RNA polymerase II transcription subunit 31 OS=Homo sapiens OX=9606 G...	[more]

BLAST of Moc10g02900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJB2	1.2e-112	100.00	Mediator of RNA polymerase II transcription subunit 31 OS=Momordica charantia OX...	[more]
A0A5D3D0Y1	2.2e-98	90.87	Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis melo var. maku...	[more]
A0A1S3BY12	2.2e-98	90.87	Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis melo OX=3656 G...	[more]
A0A0A0KZ88	7.1e-97	89.42	Mediator of RNA polymerase II transcription subunit 31 OS=Cucumis sativus OX=365...	[more]
A0A6J1JRK2	4.6e-96	88.35	Mediator of RNA polymerase II transcription subunit 31 OS=Cucurbita maxima OX=36...	[more]

BLAST of Moc10g02900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19910.1	1.3e-74	71.84	SOH1 family protein	[more]
AT5G19910.2	7.3e-70	62.71	SOH1 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008831	Mediator complex, subunit Med31	PFAM	PF05669	Med31	coord: 30..122 e-value: 4.1E-34 score: 116.5
IPR008831	Mediator complex, subunit Med31	PANTHER	PTHR13186	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31	coord: 9..203
IPR038089	Mediator of RNA polymerase II, subunit Med31 superfamily	GENE3D	1.10.10.1340	Mediator of RNA polymerase II, submodule Med31 (Soh1)	coord: 17..119 e-value: 2.9E-40 score: 138.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 177..207
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 190..207
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR13186:SF1	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31	coord: 9..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g02900.1	Moc10g02900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0070847	core mediator complex
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity