Moc10g02620 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g02620
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	gibberellin 20-oxidase-like protein
Location	chr10: 1787891 .. 1789898 (-)
RNA-Seq Expression	Moc10g02620
Synteny	Moc10g02620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGAATCTCAAACCTGTGTGGAGCTCCCAGTGATCGACGTTTATGAGGATTTGAGTGAATCGGCACTGTCTTCTCTTACGACGGCGTGCAACGAATGGGGATTCTTCTACATCAGAAATCATGGCGTCTCCAAAGAATTTTACCACAGACTCATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGAAACCAAAAACAAAGTGGGAATTTCGTGTTACGTGCCGAGGTTTATGGCGTCGCCTTTCTTTGAGAGCTTCAAATTTGCGGGGCCGGACTTCTCTGCATCTGCTTACAGCTTGACGGAAGCGCTTTTCGACCATCCCAATACAGAATTCAGGTGAGATTAAAAAAAAAATTAGGAAAAAAAAAGCGCCGAAATATCAATAATATAATTTTTAAATTAGTTCTATTAATTTCAATTTCGTCAATCAAACATATGTTATAAGTTATAACTTTGTTAAAATTTGATTATTTTTAGTACAATACGTGAATCGGGAGATCGAATTACCTGTATTAGAGTGTAGATTTTAGTAAAAACTAGAGAAAAATGAGTAGGTGTTCGGCTGGGTACAAAACAGTTTGGGGGACGATTTGTGAATTTTACCAAATCAAAGGGATTAAATTGAAAATTTCATAATCATGGATACGAAATTGAAACTAAGTTCAAAGAGATTAAATTTGTAATATGACTTTAATTTTATCAAGCTTATTTTCTTTCCTTCCTCTCTTTTTTAATCTTATTTTCTTACAAAATACGAATAAATTTTGTTTATTAGTGGTATTTAATTGAGTATAAAGTAATTATCTAATTATAATTATTGTATTTTAATTATAAAAAGTAAATCTTGTTTTGATATTAAAAACTAGATGTTTCTTTTCTCCAAAAAAAAAAATAATAATAATAATGTTTCTTTGTTTCGTAAGTATAATTTTATAAGCTTATAATTACAACGTTTACAAAAACATTTGAGAGAGGAAAAGATTAGAAAAGTTAAATTGAACATAATTTTATGAATAAGATAAAGACAATAATTATCATCTTAAAGATTAATGGTTCATATCTCACAATATAATAATTATTTTTTTTAACATTCCCACGACACAGTTATTGAACTAAAAAGAAATAAATAAATGAAGAGAATGAATTAAAAGTTATAGAGTTTATATGTACCCTTAAGGTAGAAAATGAGGGATAAAATAATTGAGAGGTAATGTAAAAGAATGAAAATTTGAAAATTACAATTTTAAAAACAGTTTTAAATACTGAAAATTATCCAACAATTTTATTTTTAAAAGTAAAAATAGAAAGCTATCATTAACAGGATGTTCTCTTTTCTTCATTCTTTTGTTTCTGATACTAATCTCTCTCTGTAAGTTGAATTTGTTTGCAGCAACCTGCTAGAGGAATATGGGAGCAAAATGAATAACCTATCAAAAAGAATAGTAGAGTTATTACTAAAGATCTTAGTAGACAAATTCGAAAACAAATTCTATGATTCTGAGTTTAGCAATTGTAAAGGTAATTTGGGAATAAACAATTATTCTCCCCGAGACTCCAACAAAGAAGACAAAGTAGAAGAAATCGAAGGACTTGGGAAGCATACCGACATTAGCTGCGTGACAATTCTATTCCAAGACAACGTCGGCGGACTTCAAATGAGATCGAAAGGAGGAGAGTGGATGGATATAAAACCTTGTGAGGACGCTCTTGTGGTGAATATTGGGGATTTTATGCAAGCTTGGAGCAATGAAAGGTTGAGATCAGCAGAGCATCGAGTTGTTTTGAGACAGAATGTGAAGAGATTCTCTATGTCATTCTTTTGGTCATATGTAGATGATAGAGAGATATATGCCCCGAGTGAAGTTGTTGGAGAAGGGAATGTGAGGCTTTATCGACCATTTTCGATTAAAGAGTATAAAATGTTTAGGGCTACCAGTGCAAGAGGGAACTACGAAAAAGTTGGAGCCACAGTAAAAGACTTTGCTGGAATTGTAGGATGA

mRNA sequence

ATGTCGGAATCTCAAACCTGTGTGGAGCTCCCAGTGATCGACGTTTATGAGGATTTGAGTGAATCGGCACTGTCTTCTCTTACGACGGCGTGCAACGAATGGGGATTCTTCTACATCAGAAATCATGGCGTCTCCAAAGAATTTTACCACAGACTCATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGAAACCAAAAACAAAGTGGGAATTTCGTGTTACGTGCCGAGGTTTATGGCGTCGCCTTTCTTTGAGAGCTTCAAATTTGCGGGGCCGGACTTCTCTGCATCTGCTTACAGCTTGACGGAAGCGCTTTTCGACCATCCCAATACAGAATTCAGCAACCTGCTAGAGGAATATGGGAGCAAAATGAATAACCTATCAAAAAGAATAGTAGAGTTATTACTAAAGATCTTAGTAGACAAATTCGAAAACAAATTCTATGATTCTGAGTTTAGCAATTGTAAAGGTAATTTGGGAATAAACAATTATTCTCCCCGAGACTCCAACAAAGAAGACAAAGTAGAAGAAATCGAAGGACTTGGGAAGCATACCGACATTAGCTGCGTGACAATTCTATTCCAAGACAACGTCGGCGGACTTCAAATGAGATCGAAAGGAGGAGAGTGGATGGATATAAAACCTTGTGAGGACGCTCTTGTGGTGAATATTGGGGATTTTATGCAAGCTTGGAGCAATGAAAGGTTGAGATCAGCAGAGCATCGAGTTGTTTTGAGACAGAATGTGAAGAGATTCTCTATGTCATTCTTTTGGTCATATGTAGATGATAGAGAGATATATGCCCCGAGTGAAGTTGTTGGAGAAGGGAATGTGAGGCTTTATCGACCATTTTCGATTAAAGAGTATAAAATGTTTAGGGCTACCAGTGCAAGAGGGAACTACGAAAAAGTTGGAGCCACAGTAAAAGACTTTGCTGGAATTGTAGGATGA

Coding sequence (CDS)

ATGTCGGAATCTCAAACCTGTGTGGAGCTCCCAGTGATCGACGTTTATGAGGATTTGAGTGAATCGGCACTGTCTTCTCTTACGACGGCGTGCAACGAATGGGGATTCTTCTACATCAGAAATCATGGCGTCTCCAAAGAATTTTACCACAGACTCATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGAAACCAAAAACAAAGTGGGAATTTCGTGTTACGTGCCGAGGTTTATGGCGTCGCCTTTCTTTGAGAGCTTCAAATTTGCGGGGCCGGACTTCTCTGCATCTGCTTACAGCTTGACGGAAGCGCTTTTCGACCATCCCAATACAGAATTCAGCAACCTGCTAGAGGAATATGGGAGCAAAATGAATAACCTATCAAAAAGAATAGTAGAGTTATTACTAAAGATCTTAGTAGACAAATTCGAAAACAAATTCTATGATTCTGAGTTTAGCAATTGTAAAGGTAATTTGGGAATAAACAATTATTCTCCCCGAGACTCCAACAAAGAAGACAAAGTAGAAGAAATCGAAGGACTTGGGAAGCATACCGACATTAGCTGCGTGACAATTCTATTCCAAGACAACGTCGGCGGACTTCAAATGAGATCGAAAGGAGGAGAGTGGATGGATATAAAACCTTGTGAGGACGCTCTTGTGGTGAATATTGGGGATTTTATGCAAGCTTGGAGCAATGAAAGGTTGAGATCAGCAGAGCATCGAGTTGTTTTGAGACAGAATGTGAAGAGATTCTCTATGTCATTCTTTTGGTCATATGTAGATGATAGAGAGATATATGCCCCGAGTGAAGTTGTTGGAGAAGGGAATGTGAGGCTTTATCGACCATTTTCGATTAAAGAGTATAAAATGTTTAGGGCTACCAGTGCAAGAGGGAACTACGAAAAAGTTGGAGCCACAGTAAAAGACTTTGCTGGAATTGTAGGATGA

Protein sequence

MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFRLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARGNYEKVGATVKDFAGIVG

Homology

[Close]

BLAST of Moc10g02620 vs. NCBI nr
Match: XP_022141253.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 648.7 bits (1672), Expect = 2.7e-182
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF
Sbjct: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120
           RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE
Sbjct: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120

Query: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180
           YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE
Sbjct: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180

Query: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300
           EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG
Sbjct: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300

Query: 301 NYEKVGATVKDFAGIVG 318
           NYEKVGATVKDFAGIVG
Sbjct: 301 NYEKVGATVKDFAGIVG 317

[Close]

BLAST of Moc10g02620 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 263/317 (82.97%), Postives = 279/317 (88.01%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+FSNLLEE
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120

Query: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180
           YGSKM +LSKRIV+LLLK+L D FENKFYD EF NC G L INNYSPRDSN     EE+E
Sbjct: 121 YGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSN-----EEVE 180

Query: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300

Query: 301 NYEKVGATVKDFAGIVG 318
            YEKVGA VK+FAGIVG
Sbjct: 301 TYEKVGANVKEFAGIVG 312

[Close]

BLAST of Moc10g02620 vs. NCBI nr
Match: XP_022140867.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 515.4 bits (1326), Expect = 3.5e-142
Identity = 255/321 (79.44%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 1   MSESQT-CVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADEL 60
           MSES++  VELPVIDVYEDLSESALSSL+ AC+EWGFFYIRNHGVSKEFY +LISIAD+L
Sbjct: 1   MSESKSYTVELPVIDVYEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 60

Query: 61  FRLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLE 120
           F LP ETKNKVG SCY+PRF+ASPF+ESFKF+GPDFSASA+ LTE L    + EFSNLL+
Sbjct: 61  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLLGKHDAEFSNLLK 120

Query: 121 EYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEI 180
           EYGSKM ++S+RIV+LLLKIL D FENKFYDSEFSNC G L +NNYSP+DSN+  K+EE+
Sbjct: 121 EYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIEEV 180

Query: 181 EGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRS 240
           EGLGKHTDISCVTILFQD+VGGLQMRSK GEWMDIKPCEDALVVNIGDFM AWSNE+LRS
Sbjct: 181 EGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKLRS 240

Query: 241 AEHRVVLRQN-VKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRAT-- 300
           AEHRVVLRQN VKRFSM F WS+ DD+EIYAPSEVVGEGN RLY+PF  KEY++FRAT  
Sbjct: 241 AEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRATPR 300

Query: 301 SARGNYEKVGATVKDFAGIVG 318
            AR NYEKVGA V DFAGIVG
Sbjct: 301 GARENYEKVGANVTDFAGIVG 321

[Close]

BLAST of Moc10g02620 vs. NCBI nr
Match: XP_022141255.1 (gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia])

HSP 1 Score: 441.0 bits (1133), Expect = 8.4e-120
Identity = 227/317 (71.61%), Postives = 240/317 (75.71%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+F      
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFR----- 120

Query: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180
                                                    INNYSPRDSN     EE+E
Sbjct: 121 -----------------------------------------INNYSPRDSN-----EEVE 180

Query: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 266

Query: 301 NYEKVGATVKDFAGIVG 318
            YEKVGA VK+FAGIVG
Sbjct: 301 TYEKVGANVKEFAGIVG 266

[Close]

BLAST of Moc10g02620 vs. NCBI nr
Match: XP_031265650.1 (gibberellin 20-oxidase-like protein [Pistacia vera])

HSP 1 Score: 388.7 bits (997), Expect = 5.0e-104
Identity = 190/319 (59.56%), Postives = 237/319 (74.29%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           M+ESQT VELPV+D+ + LS S LS+L+ AC EWGFFYI NHGVSK+ Y +L S+++ LF
Sbjct: 1   MAESQTTVELPVLDISQPLSTSKLSALSAACKEWGFFYITNHGVSKDLYRKLHSLSNHLF 60

Query: 61  RLPQETKNKVG----ISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSN 120
            LP ETK KVG    I  Y P F+ASPFFESF+ +GPDFS+SA S  EAL   PN++FS 
Sbjct: 61  NLPYETKVKVGPFSSIKTYTPHFIASPFFESFRVSGPDFSSSAQSSVEALLKEPNSDFSE 120

Query: 121 LLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKV 180
            L+EYG KM  LSK IV+ +L  L   F+ KFY+SEFS C G L I NYSP ++  E   
Sbjct: 121 TLKEYGRKMTELSKAIVQFVLMSLGKDFQKKFYESEFSKCHGYLRIINYSPPENVVE--- 180

Query: 181 EEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNER 240
           +++EGLG HTD+SCVTI++QD +GGLQ+RSK G WMDI PCED LVVNIGD M AWSN R
Sbjct: 181 KQVEGLGMHTDMSCVTIVYQDEIGGLQVRSKEGTWMDINPCEDTLVVNIGDLMHAWSNGR 240

Query: 241 LRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRAT 300
           LRS+EHRVVL++N  RFS++FFW + D++ I AP EVVGE + R+Y+ F  K+Y  FR +
Sbjct: 241 LRSSEHRVVLKRNANRFSLAFFWCFEDEKNISAPDEVVGEEDNRVYKTFICKDYLRFRES 300

Query: 301 SARGNYEKVGATVKDFAGI 316
           S +G +EKVG TVK FAGI
Sbjct: 301 SEKGKFEKVGFTVKQFAGI 316

[Close]

BLAST of Moc10g02620 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-82
Identity = 157/320 (49.06%), Postives = 221/320 (69.06%), Query Frame = 0


Query: 8   VELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFRLPQETK 67
           +ELPV D+ + LSES+L+SL  AC EWGFFY+ NHGVS++ Y +L   +  +F L  E K
Sbjct: 3   LELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 62

Query: 68  NKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDH-PNTEFSNLLEEYGSKMN 127
            K+G S Y PRF+ASPFFES + +GPDF ASA S  +A  D   + EFS L++EYG KM 
Sbjct: 63  MKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEYGEKMT 122

Query: 128 NLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYS-PRDSNKE----DKVEEIEG 187
            L ++I++ +L    D   +K+Y+SEF NC G   INNY+ P D   +    D+ + IEG
Sbjct: 123 KLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLIEG 182

Query: 188 LGKHTDISCVTILFQDNVGGLQMRSKGG-EWMDIKPCEDALVVNIGDFMQAWSNERLRSA 247
           LG HTD+SC+TI+ QD++GGLQ+R++ G   MDI P ++ALVVN+GD + AW+N RLRS+
Sbjct: 183 LGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLRSS 242

Query: 248 EHRVVLRQN---VKRFSMSFFWSYVDDREIYAPSEVVG--EGNVRLYRPFSIKEYKMFRA 307
           +HRV+L++      RFS++FFW + D + ++AP EVVG  EG +R++R F   +Y  FR 
Sbjct: 243 QHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRFRE 302

Query: 308 TSARGNYEKVGATVKDFAGI 316
           ++ +G +EKVG TV+DFA I
Sbjct: 303 SNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Moc10g02620 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 3.8e-30
Identity = 98/299 (32.78%), Postives = 144/299 (48.16%), Query Frame = 0


Query: 8   VELPVIDV------YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFR 67
           VELPVIDV       E+  E    ++  A  EWGFF + NHG+S +   ++      +FR
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 68  LPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSN----- 127
            P + K+K        +F A     S+++  P  ++         F  P T+ S+     
Sbjct: 100 EPFDKKSK------SEKFSAG----SYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFT 159

Query: 128 LLEEYGSKMNNLSKRIVELLLKILVDKF-ENKFYDSEFSNCKGN---LGINNYSPRDSNK 187
            L     K  + S+ +  +L ++L +K  +N  +  E  NC  N   L +N Y P     
Sbjct: 160 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKE--NCVRNTCYLRMNRYPPC---- 219

Query: 188 EDKVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAW 247
             K  E+ GL  HTD   +TIL+QD VGGLQ+  K   W+ +KP   AL++NIGD  QAW
Sbjct: 220 -PKPSEVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAW 279

Query: 248 SNERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYK 292
           SN   +S EHRV+    V+RFS ++F     D  I   S+         YR FS +E++
Sbjct: 280 SNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPA------YRNFSFREFR 314

[Close]

BLAST of Moc10g02620 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.4e-30
Identity = 93/315 (29.52%), Postives = 151/315 (47.94%), Query Frame = 0


Query: 8   VELPVIDV------YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFR 67
           ++LPVID+       E   +  +  +  A  EWGFF I NHG+ K+ +  ++    +LF 
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 68  LPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASA-YSLTEAL---------FDHPN 127
            P   K        V    +     S+++  P  ++ A YS++EA               
Sbjct: 98  QPFSVK--------VRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDR 157

Query: 128 TEFSNLLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSN 187
                ++E Y  ++  +++ I E+L K +     ++++++ F      L +N Y P    
Sbjct: 158 NNLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG 217

Query: 188 KEDKVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQA 247
                 E+ GL  HTD S +TIL QD +GGL++ +  G+W+ +KPC +AL VNIGD  QA
Sbjct: 218 -----SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALTVNIGDMFQA 277

Query: 248 WSNERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYK 307
            SN   +S  HRV+   N++R S++FF         Y  +E+   G  + YR FS +EYK
Sbjct: 278 LSNGVYQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKYRRFSFREYK 330

[Close]

BLAST of Moc10g02620 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-27
Identity = 96/293 (32.76%), Postives = 135/293 (46.08%), Query Frame = 0


Query: 10  LPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFRLPQETKNK 69
           +PVID+     +S   ++  A  EWGFF + NHGVS E    + +     F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  VGISCYVPRFMASPFFESFKFAGPDFSASAYSLTE-----ALFDHPNTEFSNL----LEE 129
                    F       +    G  FS  A    E     +LF     E S L       
Sbjct: 122 ---------FSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRS 181

Query: 130 YGSKMNNLSKRIVELLLKILVDKFENKFYD-SEFSNCKGNLGIN-NYSPRDSNKEDKVEE 189
              +  N +K +V+ LL+ L +    K  D ++ S   G+  IN NY P   N E  V  
Sbjct: 182 ETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPELTV-- 241

Query: 190 IEGLGKHTDISCVTILFQDNVGGLQMRS-KGGEWMDIKPCEDALVVNIGDFMQAWSNERL 249
             G+G+H+D+S +TIL QD +GGL +RS   G W+ + P   +LV+NIGD MQ  SN R 
Sbjct: 242 --GVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGSLVINIGDAMQIMSNGRY 301

Query: 250 RSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEY 291
           +S EHRV+   +  R S+  F S   +  I    EV+  G   +Y+     +Y
Sbjct: 302 KSVEHRVLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYTDY 341

[Close]

BLAST of Moc10g02620 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-27
Identity = 98/314 (31.21%), Postives = 139/314 (44.27%), Query Frame = 0


Query: 3   ESQTCVELPVIDVYEDLSESALSS------LTTACNEWGFFYIRNHGVSKEFYHRLISIA 62
           ++ T   +P+ID+    SE  LS       ++ AC  WGFF + NHGV  E         
Sbjct: 55  DAPTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENW 114

Query: 63  DELFRLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDH------- 122
            E F +P   K     S   PR             G     S Y     L  H       
Sbjct: 115 REFFHMPVNAKETYSNS---PRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKW 174

Query: 123 ---PNTEFSNLLEEYGSKMNNLSKRIVELL---LKILVDKFENKFYDSEFSNCKGNLGIN 182
              P T    +++EYG ++  LS RI+ +L   L +  DKF+  F       C   L +N
Sbjct: 175 PSFPPT-IREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGAC---LRVN 234

Query: 183 NYSPRDSNKEDKVEEIEGLGKHTDISCVTILF-QDNVGGLQMRSKGGEWMDIKPCEDALV 242
            Y      K  + E   GL  H+D   +TIL   D V GLQ+R K   W+ +KP   A +
Sbjct: 235 YYP-----KCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVR-KDDTWITVKPHPHAFI 294

Query: 243 VNIGDFMQAWSNERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLY 297
           VNIGD +Q  SN   +S EHRV++  + +R S++FF++   D  I    E+V   N  LY
Sbjct: 295 VNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLY 354

[Close]

BLAST of Moc10g02620 vs. ExPASy TrEMBL
Match: A0A6J1CJY7 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011696 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.3e-182
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF
Sbjct: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120
           RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE
Sbjct: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120

Query: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180
           YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE
Sbjct: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180

Query: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300
           EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG
Sbjct: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300

Query: 301 NYEKVGATVKDFAGIVG 318
           NYEKVGATVKDFAGIVG
Sbjct: 301 NYEKVGATVKDFAGIVG 317

[Close]

BLAST of Moc10g02620 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.3e-147
Identity = 263/317 (82.97%), Postives = 279/317 (88.01%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+FSNLLEE
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120

Query: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180
           YGSKM +LSKRIV+LLLK+L D FENKFYD EF NC G L INNYSPRDSN     EE+E
Sbjct: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSN-----EEVE 180

Query: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300

Query: 301 NYEKVGATVKDFAGIVG 318
            YEKVGA VK+FAGIVG
Sbjct: 301 TYEKVGANVKEFAGIVG 312

[Close]

BLAST of Moc10g02620 vs. ExPASy TrEMBL
Match: A0A6J1CI97 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011430 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.7e-142
Identity = 255/321 (79.44%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 1   MSESQT-CVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADEL 60
           MSES++  VELPVIDVYEDLSESALSSL+ AC+EWGFFYIRNHGVSKEFY +LISIAD+L
Sbjct: 1   MSESKSYTVELPVIDVYEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 60

Query: 61  FRLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLE 120
           F LP ETKNKVG SCY+PRF+ASPF+ESFKF+GPDFSASA+ LTE L    + EFSNLL+
Sbjct: 61  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLLGKHDAEFSNLLK 120

Query: 121 EYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEI 180
           EYGSKM ++S+RIV+LLLKIL D FENKFYDSEFSNC G L +NNYSP+DSN+  K+EE+
Sbjct: 121 EYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIEEV 180

Query: 181 EGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRS 240
           EGLGKHTDISCVTILFQD+VGGLQMRSK GEWMDIKPCEDALVVNIGDFM AWSNE+LRS
Sbjct: 181 EGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKLRS 240

Query: 241 AEHRVVLRQN-VKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRAT-- 300
           AEHRVVLRQN VKRFSM F WS+ DD+EIYAPSEVVGEGN RLY+PF  KEY++FRAT  
Sbjct: 241 AEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRATPR 300

Query: 301 SARGNYEKVGATVKDFAGIVG 318
            AR NYEKVGA V DFAGIVG
Sbjct: 301 GARENYEKVGANVTDFAGIVG 321

[Close]

BLAST of Moc10g02620 vs. ExPASy TrEMBL
Match: A0A6J1CHI8 (gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 4.1e-120
Identity = 227/317 (71.61%), Postives = 240/317 (75.71%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+F      
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFR----- 120

Query: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180
                                                    INNYSPRDSN     EE+E
Sbjct: 121 -----------------------------------------INNYSPRDSN-----EEVE 180

Query: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 266

Query: 301 NYEKVGATVKDFAGIVG 318
            YEKVGA VK+FAGIVG
Sbjct: 301 TYEKVGANVKEFAGIVG 266

[Close]

BLAST of Moc10g02620 vs. ExPASy TrEMBL
Match: A0A061FZ75 (2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao OX=3641 GN=TCM_014890 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.2e-103
Identity = 185/318 (58.18%), Postives = 240/318 (75.47%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           M E++T VELPV D+ + LS S+LSSL+ AC EWGFF I NHGVSK+ Y +L S+++ LF
Sbjct: 1   MPETKTSVELPVFDLSQPLSPSSLSSLSLACREWGFFRITNHGVSKDLYRKLYSLSNHLF 60

Query: 61  RLPQETKNKVG----ISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSN 120
            LP E+K K+G    +  Y P F+ASPFFES + +GPDF ASA S  + LF+ PN+EFS 
Sbjct: 61  SLPDESKLKLGPTSSLKTYTPHFIASPFFESLRVSGPDFFASAQSSVDVLFEQPNSEFSE 120

Query: 121 LLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKV 180
           +L+EYGS M  LSK IVE +LK L ++ E K ++SEF NC G L INNY+P  S KE   
Sbjct: 121 VLKEYGSLMTKLSKEIVEAILKSLGEELEKKSFESEFGNCHGYLRINNYTPPKSMKE--- 180

Query: 181 EEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNER 240
           EE++GLG HTD+SCVTI++QD +GGLQ+RSK G+WMDI PCED LVVNIGD M AWSN +
Sbjct: 181 EEVDGLGMHTDMSCVTIVYQDEIGGLQVRSKEGKWMDINPCEDTLVVNIGDLMHAWSNGK 240

Query: 241 LRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRAT 300
           L S+EHRVVL++ V RFS++FFW + D++ I+AP+EVVGE N+R+Y+PF   +Y  FR +
Sbjct: 241 LSSSEHRVVLKRIVNRFSLAFFWCFEDEKVIFAPNEVVGEDNLRIYKPFVCADYLKFRQS 300

Query: 301 SARGNYEKVGATVKDFAG 315
           S +G ++KVG TVKDFAG
Sbjct: 301 SEKGRFDKVGFTVKDFAG 315

[Close]

BLAST of Moc10g02620 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 342.8 bits (878), Expect = 2.9e-94
Identity = 164/317 (51.74%), Postives = 228/317 (71.92%), Query Frame = 0


Query: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60
           MSE  + ++LPV+D+ + +  S LSSL+ AC EWGFFY+ NHG+SKE + ++ S++ ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALF-DHPNTEFSNLLE 120
           + P E+K K+G   Y PR++ASP+FES   +GPDFS SA +  + LF DH   E    ++
Sbjct: 61  KAPLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETMQ 120

Query: 121 EYGSKMNNLSKRIVELLLKI-LVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEE 180
           EYG+KM  LSKR++++LL + L D+   + Y ++FSNC G L + NY+P   + E + E 
Sbjct: 121 EYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTP-PHDVEKQEEL 180

Query: 181 IEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLR 240
           +EGLG HTD+SC+TI++QD+VGGLQMRSK G+W+DI PC D LVVNIGD MQAWSN RLR
Sbjct: 181 VEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRLR 240

Query: 241 SAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSA 300
           S+EHRVVLR+ V R S++FF  + D++ I AP E+VGEG  R Y+ F   EY  FR ++ 
Sbjct: 241 SSEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNE 300

Query: 301 RGNYEKVGATVKDFAGI 316
            G +EK+G TVKDFAG+
Sbjct: 301 EGKFEKIGFTVKDFAGL 316

[Close]

BLAST of Moc10g02620 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 8.0e-84
Identity = 157/320 (49.06%), Postives = 221/320 (69.06%), Query Frame = 0


Query: 8   VELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFRLPQETK 67
           +ELPV D+ + LSES+L+SL  AC EWGFFY+ NHGVS++ Y +L   +  +F L  E K
Sbjct: 3   LELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 62

Query: 68  NKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDH-PNTEFSNLLEEYGSKMN 127
            K+G S Y PRF+ASPFFES + +GPDF ASA S  +A  D   + EFS L++EYG KM 
Sbjct: 63  MKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEYGEKMT 122

Query: 128 NLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYS-PRDSNKE----DKVEEIEG 187
            L ++I++ +L    D   +K+Y+SEF NC G   INNY+ P D   +    D+ + IEG
Sbjct: 123 KLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLIEG 182

Query: 188 LGKHTDISCVTILFQDNVGGLQMRSKGG-EWMDIKPCEDALVVNIGDFMQAWSNERLRSA 247
           LG HTD+SC+TI+ QD++GGLQ+R++ G   MDI P ++ALVVN+GD + AW+N RLRS+
Sbjct: 183 LGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLRSS 242

Query: 248 EHRVVLRQN---VKRFSMSFFWSYVDDREIYAPSEVVG--EGNVRLYRPFSIKEYKMFRA 307
           +HRV+L++      RFS++FFW + D + ++AP EVVG  EG +R++R F   +Y  FR 
Sbjct: 243 QHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRFRE 302

Query: 308 TSARGNYEKVGATVKDFAGI 316
           ++ +G +EKVG TV+DFA I
Sbjct: 303 SNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Moc10g02620 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.1e-60
Identity = 110/199 (55.28%), Postives = 148/199 (74.37%), Query Frame = 0


Query: 118 LEEYGSKMNNLSKRIVELLLKI-LVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKV 177
           ++EYG+KM  LSKR++++LL + L D+   + Y ++FSNC G L + NY+P   + E + 
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTP-PHDVEKQE 60

Query: 178 EEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNER 237
           E +EGLG HTD+SC+TI++QD+VGGLQMRSK G+W+DI PC D LVVNIGD MQAWSN R
Sbjct: 61  ELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGR 120

Query: 238 LRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRAT 297
           LRS+EHRVVLR+ V R S++FF  + D++ I AP E+VGEG  R Y+ F   EY  FR +
Sbjct: 121 LRSSEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQS 180

Query: 298 SARGNYEKVGATVKDFAGI 316
           +  G +EK+G TVKDFAG+
Sbjct: 181 NEEGKFEKIGFTVKDFAGL 198

[Close]

BLAST of Moc10g02620 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 133.7 bits (335), Expect = 2.7e-31
Identity = 98/299 (32.78%), Postives = 144/299 (48.16%), Query Frame = 0


Query: 8   VELPVIDV------YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFR 67
           VELPVIDV       E+  E    ++  A  EWGFF + NHG+S +   ++      +FR
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 68  LPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSN----- 127
            P + K+K        +F A     S+++  P  ++         F  P T+ S+     
Sbjct: 100 EPFDKKSK------SEKFSAG----SYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFT 159

Query: 128 LLEEYGSKMNNLSKRIVELLLKILVDKF-ENKFYDSEFSNCKGN---LGINNYSPRDSNK 187
            L     K  + S+ +  +L ++L +K  +N  +  E  NC  N   L +N Y P     
Sbjct: 160 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKE--NCVRNTCYLRMNRYPPC---- 219

Query: 188 EDKVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAW 247
             K  E+ GL  HTD   +TIL+QD VGGLQ+  K   W+ +KP   AL++NIGD  QAW
Sbjct: 220 -PKPSEVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAW 279

Query: 248 SNERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYK 292
           SN   +S EHRV+    V+RFS ++F     D  I   S+         YR FS +E++
Sbjct: 280 SNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPA------YRNFSFREFR 314

[Close]

BLAST of Moc10g02620 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 132.5 bits (332), Expect = 6.0e-31
Identity = 93/315 (29.52%), Postives = 151/315 (47.94%), Query Frame = 0


Query: 8   VELPVIDV------YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELFR 67
           ++LPVID+       E   +  +  +  A  EWGFF I NHG+ K+ +  ++    +LF 
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 68  LPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASA-YSLTEAL---------FDHPN 127
            P   K        V    +     S+++  P  ++ A YS++EA               
Sbjct: 98  QPFSVK--------VRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDR 157

Query: 128 TEFSNLLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSN 187
                ++E Y  ++  +++ I E+L K +     ++++++ F      L +N Y P    
Sbjct: 158 NNLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG 217

Query: 188 KEDKVEEIEGLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQA 247
                 E+ GL  HTD S +TIL QD +GGL++ +  G+W+ +KPC +AL VNIGD  QA
Sbjct: 218 -----SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALTVNIGDMFQA 277

Query: 248 WSNERLRSAEHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYK 307
            SN   +S  HRV+   N++R S++FF         Y  +E+   G  + YR FS +EYK
Sbjct: 278 LSNGVYQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKYRRFSFREYK 330

The following BLAST results are available for this feature:

BLAST of Moc10g02620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141253.1	2.7e-182	100.00	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022141254.1	2.1e-147	82.97	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]
XP_022140867.1	3.5e-142	79.44	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022141255.1	8.4e-120	71.61	gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia]	[more]
XP_031265650.1	5.0e-104	59.56	gibberellin 20-oxidase-like protein [Pistacia vera]	[more]

BLAST of Moc10g02620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	1.1e-82	49.06	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
O49561	3.8e-30	32.78	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C6I4	8.4e-30	29.52	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q9C899	1.3e-27	32.76	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]
Q9FFF6	1.3e-27	31.21	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]

BLAST of Moc10g02620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJY7	1.3e-182	100.00	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110116...	[more]
A0A6J1CI26	2.3e-147	82.97	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CI97	1.7e-142	79.44	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110114...	[more]
A0A6J1CHI8	4.1e-120	71.61	gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN...	[more]
A0A061FZ75	1.2e-103	58.18	2-oxoglutarate-dependent dioxygenase OS=Theobroma cacao OX=3641 GN=TCM_014890 PE...	[more]

BLAST of Moc10g02620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	2.9e-94	51.74	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	8.0e-84	49.06	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	2.1e-60	55.28	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G21200.1	2.7e-31	32.78	gibberellin 2-oxidase 8	[more]
AT1G50960.1	6.0e-31	29.52	gibberellin 2-oxidase 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 118..138
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 2..310
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 2..310
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 6..299
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..72 e-value: 9.9E-16 score: 58.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..293 e-value: 8.7E-69 score: 234.6
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 162..258 e-value: 7.6E-22 score: 77.7
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 152..262 score: 11.000537

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g02620.1	Moc10g02620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding