Moc10g01960 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g01960
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiongibberellin 20 oxidase 2-like
Locationchr10: 1233098 .. 1236539 (-)
RNA-Seq ExpressionMoc10g01960
SyntenyMoc10g01960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGGAATGCATGACGACCCCATCACCATCACTGGAAGAGTACAAAGCCGATAATGGGGCTTTGGGAGGGTCCACCGAGTTCAAGGCCGAGTCAGTGACGCCAGAGAATTTCATATGGCCGGACGAGTTCAAGGCGCAGGACTCACTCCCGGAGCTCCATGTCCCACATATCGACATCAAGAAGTTATTATCTGGAGACGAAGGTGGCGCTGAGGAGGCCATGCGGCTCGTGGACGAGGCGTGTAGAAAGCATGGCTTCTTCGTGGTGGTCAACCATGGCGTGGACATGGGAATCATGAAAGATCTCCATAAGTGCATGGACGAGTTCTTTGAATTGCCTCTCGACCAGAAGCAGAAGGCTCACAGGAAGCTCGGAGATTCCTATGGGTACACTAGCAGCTACATTGGCAGATTCTCCGACAAACTTCCATGGAAGGAGACCTTCTCCGTTTGCTATCTTTCTCATAAAGATCACTCCTCTCTTGTGCATGACTACGTTCGCGACACTCTTGGCCAAGAATTCTCCCACCATGGGTAACCAAAAATTAAATTCAATTACTCACATCTTTTGTTTAATGTGGTAGCCTGGTCACAGTGGATCAACCCAGACAACAAGCTTCGATCATAGAGATACTGTTAGTTCACTAATGGATCGATTTGTTGTATAATGTTCAGGAAGGTGTATCAAGAGTGCTGCAAAGCAATGGCGGAGCTGAGTTTGGAGGTGGTGGAGCTTTTGGGGATGAGCCTTGGCCTGGGAAGAGAAGAGTTCAAGAAATTGTACGAGGATCATGATTCAATAATGAGGCTTAACTATTACCCAACATGCGAGAATCCAGAGCTGACGTTGGGGACAGGGCCTCACTGCGATCCCACCTCCATAACCATCCTTCACCAAGACCATGTGAGCGGCCTTCAAGTCCACGTCGATGATCAGTGGTACTCAATCCCTCCCACCCCCGATTCCTTTGTCGTCAACATCGGTGACACTTTCATGGTAACCAATTAACAAACCAATTACAATTACAATTACAATTAGAGTTCATCCTACTTGATTATTAATCACTTTCGAGTGGGTTTGTTTAATTATTGTTATAGGCTCTGACGAATGGGGTGTACAAGAGCTGCTTCCACCGAGCAGTGGTGAACTGCAAAGAAGTAAGAAAATCAGTGGCCTTCTTTCTGTGTCCGGGGGCGGAGAAATTGGTGAGGGCGCCGGAGCAGCTGGTGGAGAAGAATCCTCCGAGGAAGTTTCCAGATTTCACATGGCCGATGCTGCATGAACTCACTCAGAAGTTTTACAGGGCAGACTCCAACACTTTGAAAGCCTTCACTACTTGGCTTCAACAGCAAAAATTAGGCGCTGCCTCCACTAATAATGATAATAATTAATAATAATGTCCCCCTTCTTTAATTTGTACCTCAACAGCCCTACCACCTCTATGTGGCAGCTCTCCTTTTGGTTTAGTTTATTTATGTGTCAATAAGTTTCAGAGTTTTATTTTCTAGAGAAAAAAGAAGAAGAAGATCGTGTAGTGTTTCTCTTTATGTTGTTTGGACGCTGGCCATCAGAAAAGTTTAATAAATATTAAATACCCATGTACAAATCTTGGCTTTGTGCTTTTTTTGTTTTTGTTTTGTCACGTGCATGCTCGACACATTATATTTCATTCTTTCACTATTTGGATTATGCTCACGAAATTTTTTTATAAGAAAGACTCAAAATAATTCAACTTGATTATTTGTATTTATGTATATGAATGTTATCCTCTCTAATGTGCACTAAGATGTTTTTTCTCGGATGCAGACTTTGAAGACTAGTGTGTGTTTGTTACCGAAACGAGTAAGTTAGTTTGTTCGACTTCTAACTTGTCGAAGAATCTTTGAACTATTCAGCTTTGAACTTATCGAGGATCTTTAGCTTGTAGCTTGAATTTGTTGCTTGAAACAATTCAATTCCCAAAAGATAGTGGACGCCTCGACATTATAGTCTGATTTTCTCTTATATTGGCTAAATACTATACTAAGGCTTTTGATATTATACCTTCATATAGTAGATATGGAAGTTCAAAATCCCCGGGTTCTATATTCTGAAACAGAAAGATTCTTTCAGCAGTCATATTCAGTGACAGATTGGCAATGGCAGAGATACCATTTTTTGACATCACAATTGGATTGAAAAAGGTCCTCTTTACCTGCTTTTCCCCAGATTTTATGCTCTTTCTAACAGGAAATATTTATCTATTTTTGAAGCATGGGATGTGGATCATGGAATTTGGTCTTTCTCGCCTAGAAGACTTCTTCTTCAAAGAGAGTCGATTATATGGTCATGGAGAACCACCGAGTTCCCTCGCCCCTCGGACCTTCAAGGCTCAGACTCTCCTAGATGGGTCCTCTCCAACAACGGCCTTTTTTCGGTGCAATCTTTCAAAGGCAGTCTCTGGAACATTAGTAGTTTCACTCCCCCTTCCTTCTTCTCGGCTCTTTGGAAGCTTCTTATTCCCAAGAAGTGTAAGTTCTTTTTGTGGTGTGCTATGTACCGTGGCATTAACACTCAAGAGAAAATCCAGAAGCGCCTCCCAAACGTATATAACAGCCCTGGTCGGTGTCCCCTTTGCAAATCAGACTCGAAAACACTTGACCATCTGCTTCTTTACTACCCACCATTAGCTATAGACTCTGGAATAATCTCTTCTCCACTTTTGATATTTGCAATACAATTCCAAGACGTATCGATCATTTCATCTCAGAGTGTCTGCAAGAGAACGGTTCTGTGGAGTAATGCGATCAGTTCGACAATCTGGCATATTTGGCTTGAGAGGAACAATCGCATATTCACATATAAAGAAAAATCCTTGGCCAACATCTGGGAAGACATTATTCTATCTGTATCTCTTTGGTCATCAAAATCTTCTTTATTTTGTAATTATGATCCTTCTATAATTTCTCTAAATTGGAGAGCTTTTATTTAATCTTCCCTTCTTTGTTGTATATGTTTCTCACATGCATAATGAAGTGATCTTTTTCCAAAAAAAAATATATATATATATCTTAAAGTCTTAGTCTTCGAGTCTTCTTCTATTCTCTGAATTGGATGCTTGGCTTTGGATGATTATGCTTGTCTCTGAATGATCATCTATCCTCCAAAATGAGACGATCTCATCTATTCATAAATTAGTATTAGTTTGAGGGACATGAGAGCGAAAAAAAAGGAAAGGAAGGGATGGGGTTTCTCTCGCGTTTGGCATAGTAGGCCCCCAGCGGGAGGCTCGCACGACGGGCTATTAGTTCAGAGCATACCCTTGATAATTGCATTGTTGTGCCTAGGTTGTGAGGGCTCTCAGTCATATGATAGTTCAATGTGCTCATCAGGAGATGTGGATCATCCAGGGCACATTAGCATGACGTACTTCTCCTGTATTCAAAGCGGGGTTTGA

mRNA sequence

ATGGCGATGGAATGCATGACGACCCCATCACCATCACTGGAAGAGTACAAAGCCGATAATGGGGCTTTGGGAGGGTCCACCGAGTTCAAGGCCGAGTCAGTGACGCCAGAGAATTTCATATGGCCGGACGAGTTCAAGGCGCAGGACTCACTCCCGGAGCTCCATGTCCCACATATCGACATCAAGAAGTTATTATCTGGAGACGAAGGTGGCGCTGAGGAGGCCATGCGGCTCGTGGACGAGGCGTGTAGAAAGCATGGCTTCTTCGTGGTGGTCAACCATGGCGTGGACATGGGAATCATGAAAGATCTCCATAAGTGCATGGACGAGTTCTTTGAATTGCCTCTCGACCAGAAGCAGAAGGCTCACAGGAAGCTCGGAGATTCCTATGGGTACACTAGCAGCTACATTGGCAGATTCTCCGACAAACTTCCATGGAAGGAGACCTTCTCCGTTTGCTATCTTTCTCATAAAGATCACTCCTCTCTTGTGCATGACTACGTTCGCGACACTCTTGGCCAAGAATTCTCCCACCATGGGAAGGTGTATCAAGAGTGCTGCAAAGCAATGGCGGAGCTGAGTTTGGAGGTGGTGGAGCTTTTGGGGATGAGCCTTGGCCTGGGAAGAGAAGAGTTCAAGAAATTGTACGAGGATCATGATTCAATAATGAGGCTTAACTATTACCCAACATGCGAGAATCCAGAGCTGACGTTGGGGACAGGGCCTCACTGCGATCCCACCTCCATAACCATCCTTCACCAAGACCATGTGAGCGGCCTTCAAGTCCACGTCGATGATCAGTGGTACTCAATCCCTCCCACCCCCGATTCCTTTGTCGTCAACATCGGTGACACTTTCATGGCTCTGACGAATGGGGTGTACAAGAGCTGCTTCCACCGAGCAGTGGTGAACTGCAAAGAAGTAAGAAAATCAGTGGCCTTCTTTCTGTGTCCGGGGGCGGAGAAATTGGTGAGGGCGCCGGAGCAGCTGGTGGAGAAGAATCCTCCGAGGAAGTTTCCAGATTTCACATGGCCGATGCTGCATGAACTCACTCAGAAGAAATATTTATCTATTTTTGAAGCATGGGATGTGGATCATGGAATTTGGTCTTTCTCGCCTAGAAGACTTCTTCTTCAAAGAGAGTCGATTATATGGTCATGGAGAACCACCGAGTTCCCTCGCCCCTCGGACCTTCAAGGCTCAGACTCTCCTAGATGGGTCCTCTCCAACAACGGCCTTTTTTCGGTGCAATCTTTCAAAGGCAGTCTCTGGAACATTAGTAGTTTCACTCCCCCTTCCTTCTTCTCGGCTCTTTGGAAGCTTCTTATTCCCAAGAAGTGTAAGTTCTTTTTGTGGTGTGCTATGTACCGTGGCATTAACACTCAAGAGAAAATCCAGAAGCGCCTCCCAAACGTATATAACAGCCCTGGTCGGTGTCCCCTTTGCAAATCAGACTCGAAAACACTTGACCATCTGCTTCTTTACTACCCACCATTAGCTATAGACTCTGGAATAATCTCTTCTCCACTTTTGATATTTGCAATACAATTCCAAGACGTATCGATCATTTCATCTCAGAGTGTCTGCAAGAGAACGGTTCTGTGGAGTAATGCGATCAGTTCGACAATCTGGCATATTTGGCTTGAGAGGAACAATCGCATATTCACATATAAAGAAAAATCCTTGGCCAACATCTGGGAAGACATTATTCTATCTGTATCTCTTTGGTCATCAAAATCTTCTTTATTTTGTTGTGAGGGCTCTCAGTCATATGATAGTTCAATGTGCTCATCAGGAGATGTGGATCATCCAGGGCACATTAGCATGACGTACTTCTCCTGTATTCAAAGCGGGGTTTGA

Coding sequence (CDS)

ATGGCGATGGAATGCATGACGACCCCATCACCATCACTGGAAGAGTACAAAGCCGATAATGGGGCTTTGGGAGGGTCCACCGAGTTCAAGGCCGAGTCAGTGACGCCAGAGAATTTCATATGGCCGGACGAGTTCAAGGCGCAGGACTCACTCCCGGAGCTCCATGTCCCACATATCGACATCAAGAAGTTATTATCTGGAGACGAAGGTGGCGCTGAGGAGGCCATGCGGCTCGTGGACGAGGCGTGTAGAAAGCATGGCTTCTTCGTGGTGGTCAACCATGGCGTGGACATGGGAATCATGAAAGATCTCCATAAGTGCATGGACGAGTTCTTTGAATTGCCTCTCGACCAGAAGCAGAAGGCTCACAGGAAGCTCGGAGATTCCTATGGGTACACTAGCAGCTACATTGGCAGATTCTCCGACAAACTTCCATGGAAGGAGACCTTCTCCGTTTGCTATCTTTCTCATAAAGATCACTCCTCTCTTGTGCATGACTACGTTCGCGACACTCTTGGCCAAGAATTCTCCCACCATGGGAAGGTGTATCAAGAGTGCTGCAAAGCAATGGCGGAGCTGAGTTTGGAGGTGGTGGAGCTTTTGGGGATGAGCCTTGGCCTGGGAAGAGAAGAGTTCAAGAAATTGTACGAGGATCATGATTCAATAATGAGGCTTAACTATTACCCAACATGCGAGAATCCAGAGCTGACGTTGGGGACAGGGCCTCACTGCGATCCCACCTCCATAACCATCCTTCACCAAGACCATGTGAGCGGCCTTCAAGTCCACGTCGATGATCAGTGGTACTCAATCCCTCCCACCCCCGATTCCTTTGTCGTCAACATCGGTGACACTTTCATGGCTCTGACGAATGGGGTGTACAAGAGCTGCTTCCACCGAGCAGTGGTGAACTGCAAAGAAGTAAGAAAATCAGTGGCCTTCTTTCTGTGTCCGGGGGCGGAGAAATTGGTGAGGGCGCCGGAGCAGCTGGTGGAGAAGAATCCTCCGAGGAAGTTTCCAGATTTCACATGGCCGATGCTGCATGAACTCACTCAGAAGAAATATTTATCTATTTTTGAAGCATGGGATGTGGATCATGGAATTTGGTCTTTCTCGCCTAGAAGACTTCTTCTTCAAAGAGAGTCGATTATATGGTCATGGAGAACCACCGAGTTCCCTCGCCCCTCGGACCTTCAAGGCTCAGACTCTCCTAGATGGGTCCTCTCCAACAACGGCCTTTTTTCGGTGCAATCTTTCAAAGGCAGTCTCTGGAACATTAGTAGTTTCACTCCCCCTTCCTTCTTCTCGGCTCTTTGGAAGCTTCTTATTCCCAAGAAGTGTAAGTTCTTTTTGTGGTGTGCTATGTACCGTGGCATTAACACTCAAGAGAAAATCCAGAAGCGCCTCCCAAACGTATATAACAGCCCTGGTCGGTGTCCCCTTTGCAAATCAGACTCGAAAACACTTGACCATCTGCTTCTTTACTACCCACCATTAGCTATAGACTCTGGAATAATCTCTTCTCCACTTTTGATATTTGCAATACAATTCCAAGACGTATCGATCATTTCATCTCAGAGTGTCTGCAAGAGAACGGTTCTGTGGAGTAATGCGATCAGTTCGACAATCTGGCATATTTGGCTTGAGAGGAACAATCGCATATTCACATATAAAGAAAAATCCTTGGCCAACATCTGGGAAGACATTATTCTATCTGTATCTCTTTGGTCATCAAAATCTTCTTTATTTTGTTGTGAGGGCTCTCAGTCATATGATAGTTCAATGTGCTCATCAGGAGATGTGGATCATCCAGGGCACATTAGCATGACGTACTTCTCCTGTATTCAAAGCGGGGTTTGA

Protein sequence

MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKYLSIFEAWDVDHGIWSFSPRRLLLQRESIIWSWRTTEFPRPSDLQGSDSPRWVLSNNGLFSVQSFKGSLWNISSFTPPSFFSALWKLLIPKKCKFFLWCAMYRGINTQEKIQKRLPNVYNSPGRCPLCKSDSKTLDHLLLYYPPLAIDSGIISSPLLIFAIQFQDVSIISSQSVCKRTVLWSNAISSTIWHIWLERNNRIFTYKEKSLANIWEDIILSVSLWSSKSSLFCCEGSQSYDSSMCSSGDVDHPGHISMTYFSCIQSGV
Homology
BLAST of Moc10g01960 vs. NCBI nr
Match: XP_022141206.1 (gibberellin 20 oxidase 2-like [Momordica charantia])

HSP 1 Score: 752.3 bits (1941), Expect = 3.3e-213
Identity = 357/368 (97.01%), Postives = 357/368 (97.01%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
           MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID
Sbjct: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60

Query: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
           IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ
Sbjct: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120

Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
           KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG
Sbjct: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180

Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
           KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT
Sbjct: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240

Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
           GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300

Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY----- 360
           AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQK Y     
Sbjct: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360

Query: 361 -LSIFEAW 363
            L  F  W
Sbjct: 361 TLKAFTTW 368

BLAST of Moc10g01960 vs. NCBI nr
Match: XP_022141149.1 (gibberellin 20 oxidase 2-like [Momordica charantia])

HSP 1 Score: 651.7 bits (1680), Expect = 6.2e-183
Identity = 309/368 (83.97%), Postives = 329/368 (89.40%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
           MA+ECMT+ SP   E KADN A GGS EFKAE+V PE FIWPDE K +DS+PELHVPHID
Sbjct: 1   MAIECMTSSSP--VESKADNAAFGGSVEFKAETVVPEKFIWPDEMKPEDSVPELHVPHID 60

Query: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
           +KK LSG+EG  EEAMRLVDEACRKHGFFVVVNHGVDMGIM+DLHKCMDEFFELPLD KQ
Sbjct: 61  MKKFLSGEEGAVEEAMRLVDEACRKHGFFVVVNHGVDMGIMRDLHKCMDEFFELPLDHKQ 120

Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
           KA RK GDSYGY SS++GRFS KLPWKETFS+ YLS++DHSS VHDYV DTLGQEFSHHG
Sbjct: 121 KAQRKFGDSYGYASSFLGRFSTKLPWKETFSLRYLSNEDHSSAVHDYVCDTLGQEFSHHG 180

Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
           KVYQEC KAMAELSLE+VELLGMSLGLGREEFKK+YED+DSI+RLN+YP CENPELTLGT
Sbjct: 181 KVYQECSKAMAELSLEIVELLGMSLGLGREEFKKVYEDNDSILRLNFYPRCENPELTLGT 240

Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
           GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG+YKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGLYKSCFHR 300

Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY----- 360
           AVVNCKE RKSVAFFLCP AEKLVRAPE+LV KNPPRKFPDFTWPML ELTQK Y     
Sbjct: 301 AVVNCKEARKSVAFFLCPAAEKLVRAPEELVVKNPPRKFPDFTWPMLLELTQKFYRADSN 360

Query: 361 -LSIFEAW 363
            L  F AW
Sbjct: 361 TLEAFTAW 366

BLAST of Moc10g01960 vs. NCBI nr
Match: CAA70329.1 (gibberellin 20-oxidase [Marah macrocarpa])

HSP 1 Score: 547.4 bits (1409), Expect = 1.6e-151
Identity = 258/357 (72.27%), Postives = 297/357 (83.19%), Query Frame = 0

Query: 1   MAMECM--TTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
           MA+ CM  T  SP+ E  +    A GG+     ES  PENFIWPDEFKA    PELHVPH
Sbjct: 1   MAITCMMATESSPAEEVKREHQVAFGGA----GESSVPENFIWPDEFKATADAPELHVPH 60

Query: 61  IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
           ID+KK+LSGDE   EEA RLVDEACRKHGFFVVVNHGVD  +M  +H+CM+EFF LPLD 
Sbjct: 61  IDLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDV 120

Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSH 180
           KQKAHRK+G+++GY +S+IGRFS KLPWKETFS+ YL+H ++SS   DYV   LG EFSH
Sbjct: 121 KQKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAH-ENSSTARDYVSQVLGPEFSH 180

Query: 181 HGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTL 240
           HG+VYQEC KA+++LSL +VELLG+SLG+ RE F+K YED+DSIMR+NYYP CE PELTL
Sbjct: 181 HGEVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTL 240

Query: 241 GTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCF 300
           GTGPHCDPTSITILHQD VSGLQV+VDDQW+SIPPT DSFVVN+GDTFM+LTNGVYKSCF
Sbjct: 241 GTGPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCF 300

Query: 301 HRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY 356
           HRAVVNCKE RKS+AFFLCP  EK+VRAPE+LVEK PPRKFPD+TWPML E+TQK Y
Sbjct: 301 HRAVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYY 352

BLAST of Moc10g01960 vs. NCBI nr
Match: AAD42693.1 (gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 532.7 bits (1371), Expect = 4.2e-147
Identity = 247/355 (69.58%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
           MAM+CM +   S E     +    G +E   ES+ PENF+WPDEFKA+D  PELHVPHID
Sbjct: 1   MAMKCMMSTGESKEMKSKLHRVEFGCSE---ESMVPENFVWPDEFKAKDGAPELHVPHID 60

Query: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
           + K L+G+E   EEA RLVDEACRKHGFFV+VNHGVDM ++K +H+CMDEFF LP D KQ
Sbjct: 61  LHKFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQ 120

Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
           K+ RK G++YGY +S+IGRFS+ LPWKET S+ Y+++ + +S V D+V   +G E SH G
Sbjct: 121 KSQRKFGENYGYANSFIGRFSNNLPWKETLSLPYVANHNSTS-VQDFVSKCVGPELSHQG 180

Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
           KVYQEC KA+++LSL++VELLG+SLG+ +E+FKK YED+DSI+RLNYYP CE PELTLGT
Sbjct: 181 KVYQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGT 240

Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
           GPHCDPTSITILHQDHVSGLQV+VDD+W+SIPPT DSFV+NIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHR 300

Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY 356
           AVVNCKEVRKS+AFFLCP A+K+VRAP+++VEKNPPRKFPD+TWPML ELTQK Y
Sbjct: 301 AVVNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFY 351

BLAST of Moc10g01960 vs. NCBI nr
Match: XP_022922616.1 (gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532.3 bits (1370), Expect = 5.4e-147
Identity = 247/362 (68.23%), Postives = 301/362 (83.15%), Query Frame = 0

Query: 1   MAMECMTT-----PSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELH 60
           MA++CMTT      S   +E K  +    GS++   ES+ PENFIWP+EFKA++S+PELH
Sbjct: 1   MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSD---ESMVPENFIWPEEFKARESVPELH 60

Query: 61  VPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELP 120
           VPHID++K +S +E   EE  RLVDEACRKHGFFV+VNHGVDM +MK++H+CMDEFF LP
Sbjct: 61  VPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLP 120

Query: 121 LDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQE 180
           LD KQKA RK+G++YGY +S+IGRFS KLPWKETFS+ Y +   H  + HDYV   LGQE
Sbjct: 121 LDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAA---HHPITHDYVCHHLGQE 180

Query: 181 FSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPE 240
           FS  GKVY+EC KA+++LSL++VELLG+SLG+ +++FKK +ED+DSI+RLNYYP C  PE
Sbjct: 181 FSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPE 240

Query: 241 LTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYK 300
           LTLGTGPHCDPTSITILHQDHVSGLQV+VDDQW+SIPP  DSFV+NIGDTFMALTNGVYK
Sbjct: 241 LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYK 300

Query: 301 SCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY 358
           SCFHRAVVNC+E RKS+ FFLCP  +K+VRAP+++V+KNPPRKFPD+TWPML ELTQK Y
Sbjct: 301 SCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFY 356

BLAST of Moc10g01960 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 3.1e-113
Identity = 198/363 (54.55%), Postives = 253/363 (69.70%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTE--------FKAESVTPENFIWPDEFKAQDSLP 60
           MA+ C TT SP+ +E++         T            +S  P  FIWPDE K    +P
Sbjct: 1   MAILC-TTTSPAEKEHEPKQDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIP 60

Query: 61  ELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFF 120
           EL+VP ID+       +    EA R++ EAC KHGFF+VVNHGV   ++ D H+ M+ FF
Sbjct: 61  ELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFF 120

Query: 121 ELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTL 180
           ++PL  KQKA RK G+S GY SS+ GRFS KLPWKET S  + +    S  V DY  DTL
Sbjct: 121 DMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTL 180

Query: 181 GQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCE 240
           GQEF   GKVYQ+ C+AM+ LSL+++ELLG+SLG+ R+ F+  +E++DSIMRLN+YP C+
Sbjct: 181 GQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQ 240

Query: 241 NPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG 300
            P+LTLGTGPHCDP+S+TILHQDHV+GLQV VD+QW SI P P +FVVNIGDTFMAL+NG
Sbjct: 241 TPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNG 300

Query: 301 VYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQ 356
           ++KSC HRAVVN +  RKS+AFFLCP  +K+V+ P  ++EK   RK+PDFTW M  E TQ
Sbjct: 301 IFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQ 357

BLAST of Moc10g01960 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.0e-108
Identity = 200/374 (53.48%), Postives = 243/374 (64.97%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKA--DNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
           MA EC+ T      E K   D+         +     P+ F+WPD  K    +  L VP 
Sbjct: 1   MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60

Query: 61  IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
           ID+   LSGD   A EA RLV +A  KHGFF++ NHGVD  ++   +  MD FF+ P  +
Sbjct: 61  IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120

Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL-SHKDHSSLVHDYVRDTLGQEFS 180
           KQKA RK G+S GY SS++GRFS KLPWKET S  +    K HS  V D+V   +G  + 
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180

Query: 181 HHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELT 240
             GKVYQE  +AM  LSL+++ELLGMSLG+ R  FK+ +ED DSI RLNYYP C+ PEL 
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240

Query: 241 LGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSC 300
           LGTGPHCDPTS+TILHQD V GLQV VD++W SIPP P +FVVNIGDTFMALTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300

Query: 301 FHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE--KNPPRKFPDFTWPMLHELTQKKY 360
            HRAVVN +  RK+ AFFLCP  EK+V+ PE+LV   K+  RK+PDFTW M  E TQK Y
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360

Query: 361 LSIFEAWDVDHGIW 370
            +     D +  IW
Sbjct: 361 RADMNTLD-EFSIW 373

BLAST of Moc10g01960 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 391.3 bits (1004), Expect = 2.0e-107
Identity = 185/333 (55.56%), Postives = 235/333 (70.57%), Query Frame = 0

Query: 36  PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
           P  FIWPD+ K   ++ EL VP ID++ LLS D     +A RL+ EAC+KHGFF+VVNHG
Sbjct: 41  PNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHG 100

Query: 96  VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
           +   ++ D H+    FF++PL +KQ+  RK G+S GY SS+ GRFS KLPWKET S  + 
Sbjct: 101 ISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFC 160

Query: 156 SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKL 215
                S  V DY  D LG  F   GKVYQE C+AM+ LSL+++ELLG+SLG+ R+ F++ 
Sbjct: 161 DDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREF 220

Query: 216 YEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTP 275
           +E++DSIMRLNYYP C  P+LTLGTGPHCDPTS+TILHQDHV+GLQV V++QW SI P P
Sbjct: 221 FEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNP 280

Query: 276 DSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNP 335
            +FVVNIGDTFMAL+N  YKSC HRAVVN +  RKS+AFFLCP  +++V  P +L++   
Sbjct: 281 KAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSIT 340

Query: 336 PRKFPDFTWPMLHELTQKKY------LSIFEAW 363
            R++PDFTW M  E TQK Y      L  F  W
Sbjct: 341 SRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDW 372

BLAST of Moc10g01960 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 2.8e-106
Identity = 186/336 (55.36%), Postives = 236/336 (70.24%), Query Frame = 0

Query: 36  PENFIWPD-EFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNH 95
           P  FIWP+ E    D+  ELHVP I+I  +LSGD   A E  RLV EAC +HGFF VVNH
Sbjct: 18  PSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGFFQVVNH 77

Query: 96  GVDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCY 155
           G+D  ++ D H+C+D FF +PL +KQ+A R+ G+S GY SS+ GRF+ KLPWKET S  +
Sbjct: 78  GIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS--F 137

Query: 156 LSHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKK 215
            S     +LV DY+  TLG++    G+VY   C  M+ LSLE++E+LG SLG+GR  +++
Sbjct: 138 RSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRR 197

Query: 216 LYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPT 275
            +E +DSIMRLNYYP C+ P  TLGTGPHCDPTS+TILHQD+V GLQVH + +W SI P 
Sbjct: 198 FFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPR 257

Query: 276 PDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKN 335
            D+FVVNIGDTFMAL+NG YKSC HRAVVN +  RKS+AFFLCP  +K+V  P  LV+ +
Sbjct: 258 ADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDAS 317

Query: 336 PPRKFPDFTWPMLHELTQKKY------LSIFEAWDV 365
            PR +PDFTW  L + TQK Y      L +F +W V
Sbjct: 318 NPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIV 351

BLAST of Moc10g01960 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 3.7e-106
Identity = 187/336 (55.65%), Postives = 234/336 (69.64%), Query Frame = 0

Query: 36  PENFIWPD-EFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNH 95
           P  FIWP+ E    D+  ELHVP IDI  +LSGD     E  RLV EAC +HGFF VVNH
Sbjct: 18  PSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNH 77

Query: 96  GVDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCY 155
           G+D  ++ D H+C+D FF +PL +KQ+A R+ G+S GY SS+ GRF+ KLPWKET S  +
Sbjct: 78  GIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS--F 137

Query: 156 LSHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKK 215
            S     +LV DY+  TLG++    G+VY   C  M+ LSLE++E+LG SLG+GR  +++
Sbjct: 138 RSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRR 197

Query: 216 LYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPT 275
            +E +DSIMRLNYYP C+ P  TLGTGPHCDPTS+TILHQD+V GLQVH + +W SI P 
Sbjct: 198 FFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPR 257

Query: 276 PDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKN 335
            D+FVVNIGDTFMAL+NG YKSC HRAVVN K  RKS+AFFLCP  +K+V  P  LV+  
Sbjct: 258 ADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAA 317

Query: 336 PPRKFPDFTWPMLHELTQKKY------LSIFEAWDV 365
            PR +PDFTW  L + TQK Y      L +F +W V
Sbjct: 318 NPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIV 351

BLAST of Moc10g01960 vs. ExPASy TrEMBL
Match: A0A6J1CIE9 (gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011662 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.6e-213
Identity = 357/368 (97.01%), Postives = 357/368 (97.01%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
           MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID
Sbjct: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60

Query: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
           IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ
Sbjct: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120

Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
           KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG
Sbjct: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180

Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
           KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT
Sbjct: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240

Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
           GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300

Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY----- 360
           AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQK Y     
Sbjct: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360

Query: 361 -LSIFEAW 363
            L  F  W
Sbjct: 361 TLKAFTTW 368

BLAST of Moc10g01960 vs. ExPASy TrEMBL
Match: A0A6J1CHR5 (gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011612 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 3.0e-183
Identity = 309/368 (83.97%), Postives = 329/368 (89.40%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
           MA+ECMT+ SP   E KADN A GGS EFKAE+V PE FIWPDE K +DS+PELHVPHID
Sbjct: 1   MAIECMTSSSP--VESKADNAAFGGSVEFKAETVVPEKFIWPDEMKPEDSVPELHVPHID 60

Query: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
           +KK LSG+EG  EEAMRLVDEACRKHGFFVVVNHGVDMGIM+DLHKCMDEFFELPLD KQ
Sbjct: 61  MKKFLSGEEGAVEEAMRLVDEACRKHGFFVVVNHGVDMGIMRDLHKCMDEFFELPLDHKQ 120

Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
           KA RK GDSYGY SS++GRFS KLPWKETFS+ YLS++DHSS VHDYV DTLGQEFSHHG
Sbjct: 121 KAQRKFGDSYGYASSFLGRFSTKLPWKETFSLRYLSNEDHSSAVHDYVCDTLGQEFSHHG 180

Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
           KVYQEC KAMAELSLE+VELLGMSLGLGREEFKK+YED+DSI+RLN+YP CENPELTLGT
Sbjct: 181 KVYQECSKAMAELSLEIVELLGMSLGLGREEFKKVYEDNDSILRLNFYPRCENPELTLGT 240

Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
           GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG+YKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGLYKSCFHR 300

Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY----- 360
           AVVNCKE RKSVAFFLCP AEKLVRAPE+LV KNPPRKFPDFTWPML ELTQK Y     
Sbjct: 301 AVVNCKEARKSVAFFLCPAAEKLVRAPEELVVKNPPRKFPDFTWPMLLELTQKFYRADSN 360

Query: 361 -LSIFEAW 363
            L  F AW
Sbjct: 361 TLEAFTAW 366

BLAST of Moc10g01960 vs. ExPASy TrEMBL
Match: Q96498 (Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 7.9e-152
Identity = 258/357 (72.27%), Postives = 297/357 (83.19%), Query Frame = 0

Query: 1   MAMECM--TTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
           MA+ CM  T  SP+ E  +    A GG+     ES  PENFIWPDEFKA    PELHVPH
Sbjct: 1   MAITCMMATESSPAEEVKREHQVAFGGA----GESSVPENFIWPDEFKATADAPELHVPH 60

Query: 61  IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
           ID+KK+LSGDE   EEA RLVDEACRKHGFFVVVNHGVD  +M  +H+CM+EFF LPLD 
Sbjct: 61  IDLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDV 120

Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSH 180
           KQKAHRK+G+++GY +S+IGRFS KLPWKETFS+ YL+H ++SS   DYV   LG EFSH
Sbjct: 121 KQKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAH-ENSSTARDYVSQVLGPEFSH 180

Query: 181 HGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTL 240
           HG+VYQEC KA+++LSL +VELLG+SLG+ RE F+K YED+DSIMR+NYYP CE PELTL
Sbjct: 181 HGEVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTL 240

Query: 241 GTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCF 300
           GTGPHCDPTSITILHQD VSGLQV+VDDQW+SIPPT DSFVVN+GDTFM+LTNGVYKSCF
Sbjct: 241 GTGPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCF 300

Query: 301 HRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY 356
           HRAVVNCKE RKS+AFFLCP  EK+VRAPE+LVEK PPRKFPD+TWPML E+TQK Y
Sbjct: 301 HRAVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYY 352

BLAST of Moc10g01960 vs. ExPASy TrEMBL
Match: Q9XHR0 (Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 2.0e-147
Identity = 247/355 (69.58%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
           MAM+CM +   S E     +    G +E   ES+ PENF+WPDEFKA+D  PELHVPHID
Sbjct: 1   MAMKCMMSTGESKEMKSKLHRVEFGCSE---ESMVPENFVWPDEFKAKDGAPELHVPHID 60

Query: 61  IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
           + K L+G+E   EEA RLVDEACRKHGFFV+VNHGVDM ++K +H+CMDEFF LP D KQ
Sbjct: 61  LHKFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQ 120

Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
           K+ RK G++YGY +S+IGRFS+ LPWKET S+ Y+++ + +S V D+V   +G E SH G
Sbjct: 121 KSQRKFGENYGYANSFIGRFSNNLPWKETLSLPYVANHNSTS-VQDFVSKCVGPELSHQG 180

Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
           KVYQEC KA+++LSL++VELLG+SLG+ +E+FKK YED+DSI+RLNYYP CE PELTLGT
Sbjct: 181 KVYQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGT 240

Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
           GPHCDPTSITILHQDHVSGLQV+VDD+W+SIPPT DSFV+NIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHR 300

Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY 356
           AVVNCKEVRKS+AFFLCP A+K+VRAP+++VEKNPPRKFPD+TWPML ELTQK Y
Sbjct: 301 AVVNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFY 351

BLAST of Moc10g01960 vs. ExPASy TrEMBL
Match: A0A6J1E4L1 (gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 2.6e-147
Identity = 247/362 (68.23%), Postives = 301/362 (83.15%), Query Frame = 0

Query: 1   MAMECMTT-----PSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELH 60
           MA++CMTT      S   +E K  +    GS++   ES+ PENFIWP+EFKA++S+PELH
Sbjct: 1   MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSD---ESMVPENFIWPEEFKARESVPELH 60

Query: 61  VPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELP 120
           VPHID++K +S +E   EE  RLVDEACRKHGFFV+VNHGVDM +MK++H+CMDEFF LP
Sbjct: 61  VPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLP 120

Query: 121 LDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQE 180
           LD KQKA RK+G++YGY +S+IGRFS KLPWKETFS+ Y +   H  + HDYV   LGQE
Sbjct: 121 LDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAA---HHPITHDYVCHHLGQE 180

Query: 181 FSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPE 240
           FS  GKVY+EC KA+++LSL++VELLG+SLG+ +++FKK +ED+DSI+RLNYYP C  PE
Sbjct: 181 FSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPE 240

Query: 241 LTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYK 300
           LTLGTGPHCDPTSITILHQDHVSGLQV+VDDQW+SIPP  DSFV+NIGDTFMALTNGVYK
Sbjct: 241 LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYK 300

Query: 301 SCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKKY 358
           SCFHRAVVNC+E RKS+ FFLCP  +K+VRAP+++V+KNPPRKFPD+TWPML ELTQK Y
Sbjct: 301 SCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFY 356

BLAST of Moc10g01960 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 410.6 bits (1054), Expect = 2.2e-114
Identity = 198/363 (54.55%), Postives = 253/363 (69.70%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKADNGALGGSTE--------FKAESVTPENFIWPDEFKAQDSLP 60
           MA+ C TT SP+ +E++         T            +S  P  FIWPDE K    +P
Sbjct: 1   MAILC-TTTSPAEKEHEPKQDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIP 60

Query: 61  ELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFF 120
           EL+VP ID+       +    EA R++ EAC KHGFF+VVNHGV   ++ D H+ M+ FF
Sbjct: 61  ELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFF 120

Query: 121 ELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTL 180
           ++PL  KQKA RK G+S GY SS+ GRFS KLPWKET S  + +    S  V DY  DTL
Sbjct: 121 DMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTL 180

Query: 181 GQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCE 240
           GQEF   GKVYQ+ C+AM+ LSL+++ELLG+SLG+ R+ F+  +E++DSIMRLN+YP C+
Sbjct: 181 GQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQ 240

Query: 241 NPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG 300
            P+LTLGTGPHCDP+S+TILHQDHV+GLQV VD+QW SI P P +FVVNIGDTFMAL+NG
Sbjct: 241 TPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNG 300

Query: 301 VYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQ 356
           ++KSC HRAVVN +  RKS+AFFLCP  +K+V+ P  ++EK   RK+PDFTW M  E TQ
Sbjct: 301 IFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQ 357

BLAST of Moc10g01960 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 395.6 bits (1015), Expect = 7.4e-110
Identity = 200/374 (53.48%), Postives = 243/374 (64.97%), Query Frame = 0

Query: 1   MAMECMTTPSPSLEEYKA--DNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
           MA EC+ T      E K   D+         +     P+ F+WPD  K    +  L VP 
Sbjct: 1   MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60

Query: 61  IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
           ID+   LSGD   A EA RLV +A  KHGFF++ NHGVD  ++   +  MD FF+ P  +
Sbjct: 61  IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120

Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL-SHKDHSSLVHDYVRDTLGQEFS 180
           KQKA RK G+S GY SS++GRFS KLPWKET S  +    K HS  V D+V   +G  + 
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180

Query: 181 HHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELT 240
             GKVYQE  +AM  LSL+++ELLGMSLG+ R  FK+ +ED DSI RLNYYP C+ PEL 
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240

Query: 241 LGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSC 300
           LGTGPHCDPTS+TILHQD V GLQV VD++W SIPP P +FVVNIGDTFMALTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300

Query: 301 FHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE--KNPPRKFPDFTWPMLHELTQKKY 360
            HRAVVN +  RK+ AFFLCP  EK+V+ PE+LV   K+  RK+PDFTW M  E TQK Y
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360

Query: 361 LSIFEAWDVDHGIW 370
            +     D +  IW
Sbjct: 361 RADMNTLD-EFSIW 373

BLAST of Moc10g01960 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-108
Identity = 185/333 (55.56%), Postives = 235/333 (70.57%), Query Frame = 0

Query: 36  PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
           P  FIWPD+ K   ++ EL VP ID++ LLS D     +A RL+ EAC+KHGFF+VVNHG
Sbjct: 41  PNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHG 100

Query: 96  VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
           +   ++ D H+    FF++PL +KQ+  RK G+S GY SS+ GRFS KLPWKET S  + 
Sbjct: 101 ISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFC 160

Query: 156 SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKL 215
                S  V DY  D LG  F   GKVYQE C+AM+ LSL+++ELLG+SLG+ R+ F++ 
Sbjct: 161 DDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREF 220

Query: 216 YEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTP 275
           +E++DSIMRLNYYP C  P+LTLGTGPHCDPTS+TILHQDHV+GLQV V++QW SI P P
Sbjct: 221 FEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNP 280

Query: 276 DSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNP 335
            +FVVNIGDTFMAL+N  YKSC HRAVVN +  RKS+AFFLCP  +++V  P +L++   
Sbjct: 281 KAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSIT 340

Query: 336 PRKFPDFTWPMLHELTQKKY------LSIFEAW 363
            R++PDFTW M  E TQK Y      L  F  W
Sbjct: 341 SRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDW 372

BLAST of Moc10g01960 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 372.5 bits (955), Expect = 6.7e-103
Identity = 175/323 (54.18%), Postives = 225/323 (69.66%), Query Frame = 0

Query: 36  PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
           P+ F+WPD  K   ++P L VP ID+   LS D     EA RLV EA +KHGFF+V NHG
Sbjct: 37  PQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNHG 96

Query: 96  VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
           VD  ++   HK MD FF+ P  +K KA RK+G++ GY SS++GRF + LPWKET S  + 
Sbjct: 97  VDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSFS 156

Query: 156 ---SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEF 215
                +++S  V +Y+  T+G  +   G VYQE  + M+ LSL+++ELLGMSLG+ RE F
Sbjct: 157 PTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREHF 216

Query: 216 KKLYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIP 275
           ++ +ED++SI RLNYYP C+ P+L LGTGPHCDPTS+TIL QD VSGLQV VD+QW SIP
Sbjct: 217 REFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSIP 276

Query: 276 PTPDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE 335
           P P + VVNIGDT MALTNG+YKSC HRAVVN +  RK++AFFLCP  +K+V+ P +L  
Sbjct: 277 PIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL-- 336

Query: 336 KNPPRKFPDFTWPMLHELTQKKY 356
               R +PDFTW M  E T K Y
Sbjct: 337 -EGERAYPDFTWSMFLEFTMKHY 356

BLAST of Moc10g01960 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 285.8 bits (730), Expect = 8.3e-77
Identity = 145/339 (42.77%), Postives = 207/339 (61.06%), Query Frame = 0

Query: 36  PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
           P  F WP++  A  S  +L +P ID+   L+G+E   + A + V +AC  HG F+VVNHG
Sbjct: 44  PAEFFWPEKDVA-PSEGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVNHG 103

Query: 96  VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
              G+ +   +    FF L  D+K +A+R  G+  GYT+ +  RFS  LPW ET ++ + 
Sbjct: 104 FKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLTLAFK 163

Query: 156 SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGL-GREEFKK 215
               H  +V D++   LG      G+V+QE C AM  L ++++ELLG+S+GL  R  +++
Sbjct: 164 KGPPH--VVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTYYRR 223

Query: 216 LYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPT 275
            +ED   I R NYYP C+ PE  LG GPH DPT+IT+L QD V GL+V     W ++ P 
Sbjct: 224 FFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTVRPR 283

Query: 276 PDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE-K 335
           P + VVN+GDTFMAL+NG Y+SC+HRAVVN ++VR+S+ FF CP  +K++  P +LVE +
Sbjct: 284 PGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELVEGE 343

Query: 336 NPPRKFPDFTWPMLHELTQKKY------LSIFEAWDVDH 367
              RK+PDFTW  L + TQ  Y      L  F +W V +
Sbjct: 344 EASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWLVSN 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141206.13.3e-21397.01gibberellin 20 oxidase 2-like [Momordica charantia][more]
XP_022141149.16.2e-18383.97gibberellin 20 oxidase 2-like [Momordica charantia][more]
CAA70329.11.6e-15172.27gibberellin 20-oxidase [Marah macrocarpa][more]
AAD42693.14.2e-14769.58gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris][more]
XP_022922616.15.4e-14768.23gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 ... [more]
Match NameE-valueIdentityDescription
Q391113.1e-11354.55Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1[more]
Q391121.0e-10853.48Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1[more]
Q391102.0e-10755.56Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2[more]
O047072.8e-10655.36Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1[more]
O047063.7e-10655.65Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CIE91.6e-21397.01gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011662 PE=... [more]
A0A6J1CHR53.0e-18383.97gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011612 PE=... [more]
Q964987.9e-15272.27Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1[more]
Q9XHR02.0e-14769.58Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1[more]
A0A6J1E4L12.6e-14768.23gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G51810.12.2e-11454.55gibberellin 20 oxidase 2 [more]
AT5G07200.17.4e-11053.48gibberellin 20-oxidase 3 [more]
AT4G25420.11.4e-10855.562-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G60980.16.7e-10354.18gibberellin 20-oxidase 4 [more]
AT1G44090.18.3e-7742.77gibberellin 20-oxidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 243..264
score: 44.63
coord: 278..304
score: 31.31
coord: 79..96
score: 56.06
NoneNo IPR availablePANTHERPTHR47990:SF26GIBBERELLIN 20-OXIDASEcoord: 29..364
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 29..364
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 34..356
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 220..318
e-value: 1.0E-27
score: 96.5
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 56..161
e-value: 2.9E-22
score: 79.6
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 430..494
e-value: 3.8E-10
score: 40.3
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 28..355
e-value: 9.2E-88
score: 296.7
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 218..318
score: 14.587361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g01960.1Moc10g01960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009908 flower development
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0009416 response to light stimulus
biological_process GO:0009826 unidimensional cell growth
molecular_function GO:0045544 gibberellin 20-oxidase activity
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding