Moc10g00850 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g00850
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	dihydrofolate synthetase isoform X1
Location	chr10: 514318 .. 519570 (-)
RNA-Seq Expression	Moc10g00850
Synteny	Moc10g00850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTCTTCAAATATCATCGCCGTTATCGCCCACAAATTCTCGGAGGGCTTCTCCTAAATTTTTTGGTGGGTGAAGGGCCTTCTATCAGTTGCAGAATAGGGTCTAAACAATGCTTTGCCACCCACTCAGAAGATCTAGAGCTAACGGAATTCATGGAATACTTGGATTCCTTGAAGAACTACGAGAAGTTGGGTGTGCCCAGAGGCGCCGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGACTGATGGAGCGGCTGGGTAATCCACAATCCAAGTTTAAGGTTGCCTCATTACCATGAACTTTTACTTTTCCTAGTCTTAACTAAATCTTGCTGTTTAGGACTAACCCACTTCCTCTCCAGTCCAAGGAGTTTAATTAGACTTGAATTTATTTGTTAAATCACCCCTTGACTTAGAAGCTTAGCCTGTGGTCACAGTAAATTTAAACCTTTATTGATTAACACTTTCCCCAGTGGAATTGGAAATTAGTGGAAGAAAATGACATTATACAAGGGTTCAAACTCAAGAGTATCACTCTATACTCTGGTACACGTTAAATCACCACTCAACCTAAAAATTTAAGTTCAAGTATGTAAATTAATCATTTATTCAGGTTCTTAACCATATTAACTATTTAGCTCTTAGTGACTAATGGTAGAAGAATTATAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGGTACAGCCTTGTTGGAAGATTTATCAGATTTTATGCATACCCTTACTTGGTAGTGATTTAGGAAGGCATAACTTTGCGTTTGTGCATTTTTTATGTGCAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAGTTTCTTGACCAGTCAGTTGAATTTGAAAATGGGCGTATTAGTCATTTCGAGGTACTAATTCATGCAGTCTGTTGTAGTGATTGGAAAAATCTCTAAGAACCCAAATGGCTTATATGCTGCTTCTGATTCTACGTGGTGTGATTAAGCAAAAGGATATTTCAGGTCTATGTTTTCGGTTGGTAAATTTGTCTGTCTCATGATCTTTTATTTCGTTCAAGATTTGAACTTCGTACATGTTTGATACCCTGCATCCTTTTGGGCTTGATTTCATGTGGGAAGGCATAAATTGTTCCAGTCAATGCTAAACCTTTCTGGGCCGTTTAAAAGTTTGTTGGACCTATGCAGTCATTAATATTTTCTTTGATCTTTTAGACGAGCTGATGAAATTCTCCAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCACAAGAGAATGTTGATGTTGCAGTCATTGAGGTTCACTTTCTTGTTGACTATTTTTTGTTTGGCAAGTATTTTTTTCTCTCTAGAACAAGCTTTCAAGGCCATTAACGATCTGTTCTAATTTTAACTGAAACAGGCTGGACTTGGTGGTGCACGAGATGCAACAAACATAATTTGCAGCTCTAGACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTAGGTGGCTCTTTGGAGAGCATTGCAATGGCAAAGGCTGGAATAATTAAACACGGTTGTCCGGTTAGGTTGTTTTTTTGATGTATAATTTTGGTGTTGATTGACTTGTTCAGCATTTGATATCCATTATCCATGCATTAGTTAAGTTTTCTCTTTTCTAATGACTTTACATGATTTACAATACCAATCCAGGATTAAATATCCTTTGAGTTTTTTGGTAACCAAATGTAGTAGGTAGGGTTAGGCAGTTGTCTCGTGAGAATACTCGAAAAAAAAGAAGAAGAAGAAGAGAAGAGAAGAGAAGAGGGGACTTTAAGTTAGCCTGAACTTACTTAAGAGTAAGCATATCACAAAAAAAGGCTCATCATAATTGTTTAAAAGATTTGTTTTTGTTGAAAGACCAGAAGAGATACAGTACAACTAAGATGCCTCAAAACTCTCCTATCTCTCTCCACGTATATCATCAAAAACATAAATCCTATTTCTAACTTTCAAAATCTCCCTTAAAATAGCTGAGGAGGGTTTCTTTGATAGTAAGATATTATATATATATATATATATCAGTGAGTGTCCGAACCAACTTGTGGCGTACCTCGAATAATTTTATGGGACAACCACCTAATCCTACTACATTTAGTTGTCAAGGTAACTCATAGGAAATATCTTACGTATGTGGCTACCATGGTTTGAACTTATTCCCTCTAAGCCATTTACTATTTACATTGGCACTTATTTGACCACAAGATCATTCCTTGATAGAAGGATTATTAACCTATTGACCTGAATAATCTCCTACTGATGAAGGACCTATTGAATACTGATTTAACTTCCTAAAGTCTATCCCCGTAAACTCTTAGGAAATAACAATGGAAAAACTTGATCTACATTGTTTCACTCTATTTTGTACACCAAACATCTTCTTGCTGTACTCTATTATTCATATTAATTCTCCTGTGAACTTTGAGCATAACAAAACTGAATTGCCATGTGGACGTTATCAAATTGATTTATTGCTGTAAGCAAACAACCTGTATGAAAAGTTCTCAGAGACATCCAAAGCCCATTCTCTGGTATTTGGCCTCCAGGGGCATGGAGTTTTCTCACCTGAAGCTGTTAACCTAAATGTATGTATTTCTGTGTTTGTGTGAGAGAGAGAGAGAGAGTTGAAGAGGAGTCCATTATTGAACTCATGTGTACTAATTCTCCAACACCTATTCTGATAATATTGTCCTTCTCATTTTATCTGATTTCTCTTCTGAAATTTATTCCATGCTCCTTGCATTTGATTTCAGACTATATTGGGTGGTCCTTTCCTTCCAAGTATTGAGTGCATTCTTCACGATAAAGCATTATCCATGTCTTCGTATGTAATATCTGCCTCATATCCTGGACATAGAAGTACTATAAAAGGTGTAAGCCTGCTTGATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAAGTATGGCTCATGTATTCTACCAAATAAATTTAAATTTCACTCCTTGAACTTCTATCAATAATTCCTAAATCTTTTTGCCAATGCAGTTCATTGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAACGCAGCAACTGCAACTTGTGCTATTCTCACTCTCCGCAATTTAGGTATAATATCATTTTTGATATTTTATTTGATATCTGTACCTTCATTTAGAGAATAAACTATTCTAATAAATAATTCAGGTTGGAGAATTTCGGATTCCTCAATTAAGACTGGACTAGAGCAAACATTCTTGGTCGGTAGGAGCCATTTCTTATCAGCAAAGGAGGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCTGTTTATTACAGTCAAATTTGGTTTGTTGGGAGATGAGGGTTTTTTTTTTCCTTTTTTATTTTATATATAATTTTAAATACCATGAACATCGAGCTAGCTTATGTGCATCGTGACCAGTCACAAGACAATTATTGGAGCCTACAACGTTTGGTTAGGAACATTTAATTGTCAAGAAATTCGTAGATAGATGGTTACTATAGTATTTTAACTTGCCTCTTGACTACTGAGCCAACCCTTGGACGTGAGCATTGTGCTTGTGCCATGTCTCCTCTACCCTACTGTACTTGAGACTTTTGGTAATTTGATGTTTGAATCATGGGTACTCTTGTAGATGTTACCGGTACATGTTTCAAATTCATGGATTGCTAATTGCTCAAAAAACTCCAGAATGTTCAAGGTCTTTTTGGTGTTGATCAAATGGGTTTAGCCGATAAAATTTCAACCTGTGTCGGCTGATATATAGGGAAGTTTATGTTGTGTTGCTCTGGTTTAGAAGTTGGTCTCATACCTTACATGCATTTTGACACCCTTTCTGAGTGGTTTCTTGAATGTTTTCTCAGCCCACACCAAAGACTCTGCCAAAGCATTAGTGGACACCATTCAGATGACTTTTCCGGAGGCACGGTTGGCTCTCGTGGTTGCAATGGCTAGCGATAAGGATCACGTTGGTTTTGCCAGAGAATTTCTTCAAGGTATAGCCTTCCCCTTCAAAGATTTCGTCCCAGATTTTCTTTGACCTACAGAGGACAGATTTCTTCTCTCCATTCACCTCGAAGAATATTGCTTGATTGTAAAATCTTCAATAGTCATGAAACTTTTGGATTATATAGGGTTTGTTTGATAACTATGTTACTTTTGGATCATTTTAAAATGTGTGCAGAGGTAGATTAGCTAAGGTTATAAAAAGAGCTTAACTTAGAGATAGTATGATCAAGATAGATCAGTGAAAGAATGTGTAAAGAGAAATAGTAAGAGAAACGCTGTGTTCAAGAGTAGAAAACAGAAAATAGGTTTTTGAAATTAGTTTTTTTTTTTGTTCTTAATACTAGTTTAGATTTCCAAAGTATTGTTGGAAAACAGAAAACTAAAAGCAAAAAAATGTCATCCAACATACTTTATAAAAACAAAAGCAGAAAACAGAGTTGTCTTCTTAACCTCTTTTATCAAATTATACGTTTCCTATTCTTTCAAATTGAGAATGTCAAATGTTTCTGCTCTTTCTTCATTGTCTTACTGCTTGGTGATTTAAACGTAATAATCACTTTCTGTAGAGATTCAATTTGAATCTGGGATATCTCGACGAAAACATAACAATCTGAGTTCCCGTCTGCCTTCTCTAATGTGTGAGATGTATAGCTATGAAATGCTGACAGTCGGCAGTTACTGAACTTGCAGGTGGAAAATTGGAGTCCATTATCTTGACTGAAGCTAATATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCCATTCTAAGAGACTGCTGGATCCAAGCAGCCAATGAATTGGGGATCCCAATTTCTCTAGAAACTAAAGATGCACTGGTCTCCTCCACAAGCAAACTCGAAAGCAGACCGATATTAACTACCGAGACCTCGTTATTACATGCCATGAAAATTGCTGCTGAGATTCTCAAGCAGAGAACGGAAGGGCGAGCAGGTCTTACCGTAGTAACTGGGTCGCTGCATGCTGTTTCAATGGTGTTATCTTCTCTCCATTCTTGA

mRNA sequence

ATGAATGTCTTCAAATATCATCGCCGTTATCGCCCACAAATTCTCGGAGGGCTTCTCCTAAATTTTTTGGTGGGTGAAGGGCCTTCTATCAGTTGCAGAATAGGGTCTAAACAATGCTTTGCCACCCACTCAGAAGATCTAGAGCTAACGGAATTCATGGAATACTTGGATTCCTTGAAGAACTACGAGAAGTTGGGTGTGCCCAGAGGCGCCGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGACTGATGGAGCGGCTGGGTAATCCACAATCCAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAGTTTCTTGACCAGTCAGTTGAATTTGAAAATGGGCGTATTAGTCATTTCGAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCACAAGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACAAACATAATTTGCAGCTCTAGACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTAGGTGGCTCTTTGGAGAGCATTGCAATGGCAAAGGCTGGAATAATTAAACACGGTTGTCCGACTATATTGGGTGGTCCTTTCCTTCCAAGTATTGAGTGCATTCTTCACGATAAAGCATTATCCATGTCTTCGTATGTAATATCTGCCTCATATCCTGGACATAGAAGTACTATAAAAGGTGTAAGCCTGCTTGATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCATTGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAACGCAGCAACTGCAACTTGTGCTATTCTCACTCTCCGCAATTTAGGTTGGAGAATTTCGGATTCCTCAATTAAGACTGGACTAGAGCAAACATTCTTGGTCGGTAGGAGCCATTTCTTATCAGCAAAGGAGGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGCCCACACCAAAGACTCTGCCAAAGCATTAGTGGACACCATTCAGATGACTTTTCCGGAGGCACGGTTGGCTCTCGTGGTTGCAATGGCTAGCGATAAGGATCACGTTGGTTTTGCCAGAGAATTTCTTCAAGGTGGAAAATTGGAGTCCATTATCTTGACTGAAGCTAATATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCCATTCTAAGAGACTGCTGGATCCAAGCAGCCAATGAATTGGGGATCCCAATTTCTCTAGAAACTAAAGATGCACTGGTCTCCTCCACAAGCAAACTCGAAAGCAGACCGATATTAACTACCGAGACCTCGTTATTACATGCCATGAAAATTGCTGCTGAGATTCTCAAGCAGAGAACGGAAGGGCGAGCAGGTCTTACCGTAGTAACTGGGTCGCTGCATGCTGTTTCAATGGTGTTATCTTCTCTCCATTCTTGA

Coding sequence (CDS)

ATGAATGTCTTCAAATATCATCGCCGTTATCGCCCACAAATTCTCGGAGGGCTTCTCCTAAATTTTTTGGTGGGTGAAGGGCCTTCTATCAGTTGCAGAATAGGGTCTAAACAATGCTTTGCCACCCACTCAGAAGATCTAGAGCTAACGGAATTCATGGAATACTTGGATTCCTTGAAGAACTACGAGAAGTTGGGTGTGCCCAGAGGCGCCGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGACTGATGGAGCGGCTGGGTAATCCACAATCCAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAGTTTCTTGACCAGTCAGTTGAATTTGAAAATGGGCGTATTAGTCATTTCGAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCACAAGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACAAACATAATTTGCAGCTCTAGACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTAGGTGGCTCTTTGGAGAGCATTGCAATGGCAAAGGCTGGAATAATTAAACACGGTTGTCCGACTATATTGGGTGGTCCTTTCCTTCCAAGTATTGAGTGCATTCTTCACGATAAAGCATTATCCATGTCTTCGTATGTAATATCTGCCTCATATCCTGGACATAGAAGTACTATAAAAGGTGTAAGCCTGCTTGATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCATTGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAACGCAGCAACTGCAACTTGTGCTATTCTCACTCTCCGCAATTTAGGTTGGAGAATTTCGGATTCCTCAATTAAGACTGGACTAGAGCAAACATTCTTGGTCGGTAGGAGCCATTTCTTATCAGCAAAGGAGGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGCCCACACCAAAGACTCTGCCAAAGCATTAGTGGACACCATTCAGATGACTTTTCCGGAGGCACGGTTGGCTCTCGTGGTTGCAATGGCTAGCGATAAGGATCACGTTGGTTTTGCCAGAGAATTTCTTCAAGGTGGAAAATTGGAGTCCATTATCTTGACTGAAGCTAATATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCCATTCTAAGAGACTGCTGGATCCAAGCAGCCAATGAATTGGGGATCCCAATTTCTCTAGAAACTAAAGATGCACTGGTCTCCTCCACAAGCAAACTCGAAAGCAGACCGATATTAACTACCGAGACCTCGTTATTACATGCCATGAAAATTGCTGCTGAGATTCTCAAGCAGAGAACGGAAGGGCGAGCAGGTCTTACCGTAGTAACTGGGTCGCTGCATGCTGTTTCAATGGTGTTATCTTCTCTCCATTCTTGA

Protein sequence

MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVSMVLSSLHS

Homology

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BLAST of Moc10g00850 vs. NCBI nr
Match: XP_022141068.1 (dihydrofolate synthetase isoform X1 [Momordica charantia])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.5e-307
Identity = 548/548 (100.00%), Postives = 548/548 (100.00%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.4 bits (2484), Expect = 3.2e-276
Identity = 489/548 (89.23%), Postives = 521/548 (95.07%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MN+FKYH  +RPQI G LLLN+ VGEGPSIS RIGSKQCF  HSED ++TEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECIL DKALSMSS V+SAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG TILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDH GFA EFLQGGKLESI+L+EANIGGGKSRTTSAA+LRDCWIQA+NE+GIP
Sbjct: 421 VVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR EGR GL VVTGSLHAVS
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIEGRRGLVVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. NCBI nr
Match: KAG6576673.1 (Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.7 bits (2477), Expect = 2.1e-275
Identity = 486/548 (88.69%), Postives = 520/548 (94.89%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MN+FKYH  +RPQI G LLLN+ VGEGPSIS RIGSKQCF  HSED ++TEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECIL DKALSMSS V+SAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG  ILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDH GFA EFLQGGKLESI+L+EANIGGGKSRTTSAA+LRDCWIQA+NE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR +GR GL VVTGSLHAVS
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDGRRGLVVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. NCBI nr
Match: XP_022922898.1 (dihydrofolate synthetase isoform X1 [Cucurbita moschata])

HSP 1 Score: 956.1 bits (2470), Expect = 1.3e-274
Identity = 485/548 (88.50%), Postives = 519/548 (94.71%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MN+FKYH  +RPQI G LLLN+ VGEGPSIS RIGSKQCF  HSED ++TEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECIL DKALSMSS V+SAS PG+RS+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG  ILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDH GFA EFLQGGKLESI+L+EANIGG KSRTTSAA+LRDCWIQA+NE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR +GR GL VVTGSLHAVS
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDGRRGLVVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 947.6 bits (2448), Expect = 4.8e-272
Identity = 488/548 (89.05%), Postives = 510/548 (93.07%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           M +FKYH   RPQILG LLLN   GE PSIS RIGSKQCF+TH ED  +TEFMEYLDSLK
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP+GAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYS GCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEF DQSVE ENGRISH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAF LFAQENVD+AVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIA AKAGIIK GCP ILGGPFLPSIE IL DKALSMSS VISAS PG+RSTIKGVSL
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+G +CQCCD+VIQID+EFIELLDVNLRMLG HQLQNAATATC IL LRNLGWRISD+S+
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLGWRISDASV 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLEQTFLVGRSHFL AKEAGVLGLPGATILLDGAHTKDSAKALVDTIQM FPEARLAL
Sbjct: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDH+GFAREFLQGGKLESI+LTEA+IGGGKSRTTSA  LRDCWIQA+NELGIP
Sbjct: 421 VVAMASDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTSAVFLRDCWIQASNELGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA VS TSKLE+RP+LTTETSLLHA+K+AAEILKQRTEGR GL VVTGSLHAVS
Sbjct: 481 ISLETKDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQRTEGRQGLVVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLS LHS
Sbjct: 541 MVLSFLHS 548

[Close]

BLAST of Moc10g00850 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.3e-160
Identity = 315/510 (61.76%), Postives = 384/510 (75.29%), Query Frame = 0


Query: 44  SEDLELTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED EL +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPFLP IE IL  KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 ISASYPGHRSTIKGVSLLDG-RLCQCCDIVIQ---IDNEFIELLDVNLRMLGRHQLQNAA 343
           I AS  G  S+IKG+   +G  LCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCAILTLRNLG-WRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHT 403
           TATC  L LR+ G  R++D +I+ GLE T L+GRS FL+ KEA  L LPGAT+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399

Query: 404 KDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKS 463
           K+SA+AL + I+  FPE RL  VVAMASDKDHV FA+E L G K E++ILTEA+IGGGK 
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459

Query: 464 RTTSAAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTET-SLLHAMKIAAE 523
           R+T +++L++ WI+AA+ELG                   SR +  +E  ++L ++K+A +
Sbjct: 460 RSTESSVLKESWIKAADELG-------------------SRSMEASENKTVLGSLKLAYK 519

Query: 524 ILK-QRTEGRAGLTVVTGSLHAVSMVLSSL 547
           IL    T   +G+ +VTGSLH VS VL+SL
Sbjct: 520 ILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of Moc10g00850 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 2.3e-43
Identity = 115/353 (32.58%), Postives = 184/353 (52.12%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRM++LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQ-ENVDV 200
           RIS+  +G  +S +   +L  + K  ++   + E G+ + FE++TA AF  FA+   VD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 201 AVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTI 260
            + E GLGG  D+TN++        VITSIG +HM  LG ++E IA  KAGIIK G P +
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIV 203

Query: 261 LGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEF 320
                  +++ I H+     + +           ++    ++                E 
Sbjct: 204 TAVTQPEALQVIRHEAERHAAPF----------QSLHDACVIFNEEALPAGEQFSFKTEE 263

Query: 321 IELLDVNLRMLGRHQLQNAATATCAI--LTLRNLGWRISDSSIKTGLEQTFLVGRSHFLS 380
               D+   ++G HQ QNAA +  A   L   N+   ISD ++++GL +    GR   + 
Sbjct: 264 KCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLELVQ 323

Query: 381 AKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDH 431
                        + LDGAH ++  + L +T++  F  +R+++V +   DK +
Sbjct: 324 EH---------PPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPY 351

[Close]

BLAST of Moc10g00850 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 9.1e-32
Identity = 112/401 (27.93%), Postives = 183/401 (45.64%), Query Frame = 0


Query: 47  LELTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGT 106
           +  TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT
Sbjct: 1   MNYTETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGT 60

Query: 107 KGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEF 166
            GKGS A  ++++L A G +VG YTSP I    ERI +    E +   AL +     +  
Sbjct: 61  NGKGSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDH--EPIPDAALVNAVAFVRAA 120

Query: 167 LDQSVEFE-NGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAV 226
           L++  + + +  ++ FE +TA+ +  F Q  VDVAVIE G+GG  D+TN+I       +V
Sbjct: 121 LERLQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDTDSTNVITP---VVSV 180

Query: 227 ITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVI- 286
           +T +  +H   LG ++ +IA  KAGIIK G P + G   +P    ++  K  +  S  + 
Sbjct: 181 LTEVALDHQKLLGHTITAIAKHKAGIIKRGIPVVTGN-LVPDAAAVVAAKVATTGSQWLR 240

Query: 287 ---SASYP-------GHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQ 346
                S P       G R T +     DGR+                  D+ + ++G +Q
Sbjct: 241 FDRDFSVPKAKLHGWGQRFTYED---QDGRIS-----------------DLEVPLVGDYQ 300

Query: 347 LQNAATA-TCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILL 406
            +N A A   A +  +   W ++  +I+ GL  +    R   +S             I++
Sbjct: 301 QRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARLEKIS---------DTPLIVI 352

Query: 407 DGAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFA 435
           DGAH  D    L+  ++  F +  + ++  + +DKD+   A
Sbjct: 361 DGAHNPDGINGLITALKQLFSQP-ITVIAGILADKDYAAMA 352

[Close]

BLAST of Moc10g00850 vs. ExPASy Swiss-Prot
Match: D4GW05 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=folCP PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 7.7e-31
Identity = 116/384 (30.21%), Postives = 177/384 (46.09%), Query Frame = 0


Query: 84  MRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERIS 143
           +RRL+  LG+P      + +AG+ GKGSTA  +  +LR  G  VG YTSPH + +RER+ 
Sbjct: 25  IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84

Query: 144 LGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIE 203
           +   G  +   AL++     K +L +    +   ++ FE +TA+A   F +  VDVAV+E
Sbjct: 85  V--DGRKIPKSALSAFVAEAKPYLVERAA-DGEPLTFFETVTALALWYFDRAGVDVAVLE 144

Query: 204 AGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILG-- 263
            G+GG  DAT+ +      A+ +T++  EH A LG ++  IA  KA +     P + G  
Sbjct: 145 VGMGGELDATSAVDP---VASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTGTT 204

Query: 264 GPFLPSI-----ECILHDKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQID 323
           G  L  I     + +    A + S   +  SY G  +             Q   + ++ D
Sbjct: 205 GEALSVIREEAGDVLTVGDADADSDADVRVSYGGRVNH------------QEAAVTVETD 264

Query: 324 NEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFL 383
               E LDV + +LG +Q +NA     A+   R +   I   +I  GL      GR   +
Sbjct: 265 ---AETLDVRIPLLGAYQARNAG---IAVSLARQVRPDIDAEAIHRGLRNAHWPGRFEVM 324

Query: 384 SAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDH---VGF 443
             +          T++LDGAH  D A A V T+   F    L LV     DKDH   VG 
Sbjct: 325 GTE---------PTVVLDGAHNPD-ACAQVATVLDEFDYDDLHLVYGAMHDKDHGEMVGA 368

Query: 444 AREFLQGGKLESIILTEANIGGGK 458
             E      + S++  +A+I  G+
Sbjct: 385 LPE------VASVVTCKADISRGE 368

[Close]

BLAST of Moc10g00850 vs. ExPASy Swiss-Prot
Match: Q9HS44 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=folP PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 8.5e-30
Identity = 107/349 (30.66%), Postives = 166/349 (47.56%), Query Frame = 0


Query: 87  LMERLGNP-QSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLG 146
           L+  LG+P Q     + IAG+ GKGSTA F  +ILRA G  VG YTSPH + +RER+++ 
Sbjct: 28  LLASLGDPHQDGPDYVQIAGSNGKGSTARFTESILRAAGLDVGLYTSPHFDDVRERVTV- 87

Query: 147 RSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAG 206
            +G  +S  AL +  +    ++ +    +    ++FEV+TAMA   F++ +VDVAV+E G
Sbjct: 88  -NGRRMSKAALTTFIETITPYVTERAA-DGAAPTYFEVVTAMALWQFSRADVDVAVLEVG 147

Query: 207 LGGARDATNIICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFL 266
           +GG  DAT+++  S   A+ +TS+  EH   LG ++  IA  KA +   G          
Sbjct: 148 IGGRLDATSVVDPS---ASAVTSVTLEHTDVLGETIPEIARDKAHVAPAG--------DT 207

Query: 267 PSIECILHDKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDV 326
           P +     D   ++  +  +    G  +     +  +GR       V    +++    ++
Sbjct: 208 PLVTATTDDALAAVRDHAGAVRTVGDTAGRDVTATYEGRTNHTEAAVSITGDDWSVATEI 267

Query: 327 NLRMLGRHQLQNAATATCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLG 386
            L  LG HQ +NA  A  A L  +  G  + D +I  GL      GR   + A       
Sbjct: 268 PL--LGDHQAENAGVA--AALARQTAG--VDDDAIARGLRSAHWPGRFEVMGA------- 327

Query: 387 LPGATILLDGAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFA 435
                ++LDGAH   +  A+ +T+   F   RL  V     DKDH   A
Sbjct: 328 --DPVVVLDGAHNPGACGAVAETV-AEFDYDRLLTVFGAMHDKDHGAMA 346

[Close]

BLAST of Moc10g00850 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.7e-307
Identity = 548/548 (100.00%), Postives = 548/548 (100.00%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP
Sbjct: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS
Sbjct: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.5e-275
Identity = 485/548 (88.50%), Postives = 519/548 (94.71%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MN+FKYH  +RPQI G LLLN+ VGEGPSIS RIGSKQCF  HSED ++TEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECIL DKALSMSS V+SAS PG+RS+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG  ILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDH GFA EFLQGGKLESI+L+EANIGG KSRTTSAA+LRDCWIQA+NE+GIP
Sbjct: 421 VVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR +GR GL VVTGSLHAVS
Sbjct: 481 ISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIDGRRGLVVVTGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 484/548 (88.32%), Postives = 511/548 (93.25%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MNVFKYH   RPQILG LLLN  +GE PSIS R GSKQCFATHSED  +TEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALNSLFKRNKE  DQSVE E+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIK GCPTILGG FLP IE IL DKALSMSS VI+ S PG+RSTIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+G LCQCCDIVIQIDNEF+ELLDVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLEQTFL+GRSHFL+AKEA VLGLPGATILLDGAHTKDSAKALVDTIQM FPEARLAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKALVDTIQMAFPEARLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           V+AMASDK+HV FAREFLQGGKLE ++LTEA+IGGGKSRTTSAA LRDCWIQA+ ELGIP
Sbjct: 421 VIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAAFLRDCWIQASIELGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA VS TSKLE+RP+LTTETSLLHA+KIAAEILKQRT+G+  L VV+GSLHAVS
Sbjct: 481 ISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTKGQQSLVVVSGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 548

[Close]

BLAST of Moc10g00850 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 1.0e-267
Identity = 480/548 (87.59%), Postives = 510/548 (93.07%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MNVFKYH   RPQILG LLLN  +GE PSIS R GSKQCFATHSED  +T+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKE  DQSVE ENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPFLP IE IL DKALSMSS VISAS PG+RSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+G L QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLEQTFL+GRSHFL+A+EA VLGLPGATILLDGAHTKDSAKAL+DTIQM FPEA+LAL
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDK+HVGFAREFLQGGKLES++LTEA IGGGKSRTTSAA LRDCWIQA+NELGIP
Sbjct: 421 VVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
           ISLETKDA V  TSKL +RP+LTTETSLLHA+KIAAEILKQRT+GR GL VV+GSLHAVS
Sbjct: 481 ISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVS 540

Query: 541 MVLSSLHS 549
           MVL+SLHS
Sbjct: 541 MVLASLHS 548

[Close]

BLAST of Moc10g00850 vs. ExPASy TrEMBL
Match: A0A6J1J6Z7 (dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.7e-262
Identity = 473/548 (86.31%), Postives = 504/548 (91.97%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60
           MNVFKYH  +R QILG LLLN+ VGEGPSIS RIGSKQCF  HSED ++TEFMEYLDSLK
Sbjct: 1   MNVFKYHHHFRLQILGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSILAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECIL DKALSMSS V+SAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ D+EFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGRLCQCCDLIIQTDDEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKALVDTIQMTFPEARLAL 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG TILLDGAHTKDSAKALVDTIQM+FP+A+LAL
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKALVDTIQMSFPDAQLAL 420

Query: 421 VVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIP 480
           VVAMASDKDH GFA EFLQ GKLESI+L+EANIGGGKSRTTSAA+LRDCWIQA+NE+GIP
Sbjct: 421 VVAMASDKDHNGFATEFLQCGKLESIVLSEANIGGGKSRTTSAALLRDCWIQASNEMGIP 480

Query: 481 ISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVS 540
                     + TS      +LTTETSLL A+KIAAEILKQRTEGR GL VVTGSLHAVS
Sbjct: 481 ----------TQTS------LLTTETSLLRAIKIAAEILKQRTEGRRGLVVVTGSLHAVS 532

Query: 541 MVLSSLHS 549
           MVLSSLHS
Sbjct: 541 MVLSSLHS 532

[Close]

BLAST of Moc10g00850 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.6e-161
Identity = 315/510 (61.76%), Postives = 384/510 (75.29%), Query Frame = 0


Query: 44  SEDLELTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED EL +F+ +L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPFLP IE IL  KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 ISASYPGHRSTIKGVSLLDG-RLCQCCDIVIQ---IDNEFIELLDVNLRMLGRHQLQNAA 343
           I AS  G  S+IKG+   +G  LCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCAILTLRNLG-WRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHT 403
           TATC  L LR+ G  R++D +I+ GLE T L+GRS FL+ KEA  L LPGAT+LLDGAHT
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHT 399

Query: 404 KDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKS 463
           K+SA+AL + I+  FPE RL  VVAMASDKDHV FA+E L G K E++ILTEA+IGGGK 
Sbjct: 400 KESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLKPEAVILTEADIGGGKI 459

Query: 464 RTTSAAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTET-SLLHAMKIAAE 523
           R+T +++L++ WI+AA+ELG                   SR +  +E  ++L ++K+A +
Sbjct: 460 RSTESSVLKESWIKAADELG-------------------SRSMEASENKTVLGSLKLAYK 519

Query: 524 ILK-QRTEGRAGLTVVTGSLHAVSMVLSSL 547
           IL    T   +G+ +VTGSLH VS VL+SL
Sbjct: 520 ILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of Moc10g00850 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 120.2 bits (300), Expect = 5.3e-27
Identity = 100/322 (31.06%), Postives = 149/322 (46.27%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E VDV ++E GLGG  DATN+
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV 175

Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDK 275
           I   +     I+S+G +HM  LG +L  IA  KAGI K G         +P+      D+
Sbjct: 176 I--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSG---------VPAFTVAQPDE 235

Query: 276 ALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLR--MLGRH 335
           A+ + +   S                           ++++ + +E LD + R  + G H
Sbjct: 236 AMRVLNEKASK--------------------------LEVNLQVVEPLDSSQRLGLQGEH 295

Query: 336 QLQNAATATCAILT-LRNLGWRISDSSIKT---------GLEQTFLVGRSHFLSAKEAGV 395
           Q  NA  A     T L+ +G    +   +T         GL   +L+GR+  +   E   
Sbjct: 296 QYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE--- 333

Query: 396 LGLPGATI-LLDGAHTKDSAKA 405
             LP   +  LDGAH+ +S +A
Sbjct: 356 --LPEEIVYYLDGAHSPESMEA 333

[Close]

BLAST of Moc10g00850 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 118.6 bits (296), Expect = 1.5e-26
Identity = 100/322 (31.06%), Postives = 148/322 (45.96%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E VDV ++E GLGG  DATNI
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNI 175

Query: 216 ICSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDK 275
               +     I+S+G +HM  LG +L  IA  KAGI K G         +P+      D+
Sbjct: 176 ---QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSG---------VPAFTVAQPDE 235

Query: 276 ALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLR--MLGRH 335
           A+ + +   S                           ++++ + +E LD + R  + G H
Sbjct: 236 AMRVLNEKASK--------------------------LEVNLQVVEPLDSSQRLGLQGEH 295

Query: 336 QLQNAATATCAILT-LRNLGWRISDSSIKT---------GLEQTFLVGRSHFLSAKEAGV 395
           Q  NA  A     T L+ +G    +   +T         GL   +L+GR+  +   E   
Sbjct: 296 QYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMIVPDSE--- 332

Query: 396 LGLPGATI-LLDGAHTKDSAKA 405
             LP   +  LDGAH+ +S +A
Sbjct: 356 --LPEEIVYYLDGAHSPESMEA 332

[Close]

BLAST of Moc10g00850 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 110.2 bits (274), Expect = 5.5e-24
Identity = 66/161 (40.99%), Postives = 88/161 (54.66%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 170

Query: 157 NSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNII 216
              F      L +    E    ++F  L  +AF +FA E VD A++E GLGG  DATN +
Sbjct: 171 LGYFWWCYNRLKERTNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV 230

Query: 217 CSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCP 258
              +     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 231 --QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 267

[Close]

BLAST of Moc10g00850 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 110.2 bits (274), Expect = 5.5e-24
Identity = 66/161 (40.99%), Postives = 88/161 (54.66%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 53  KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 112

Query: 157 NSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNII 216
              F      L +    E    ++F  L  +AF +FA E VD A++E GLGG  DATN +
Sbjct: 113 LGYFWWCYNRLKERTNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV 172

Query: 217 CSSRLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCP 258
              +     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 173 --QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 209

The following BLAST results are available for this feature:

BLAST of Moc10g00850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141068.1	3.5e-307	100.00	dihydrofolate synthetase isoform X1 [Momordica charantia]	[more]
XP_023553540.1	3.2e-276	89.23	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]
KAG6576673.1	2.1e-275	88.69	Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922898.1	1.3e-274	88.50	dihydrofolate synthetase isoform X1 [Cucurbita moschata]	[more]
XP_038905573.1	4.8e-272	89.05	dihydrofolate synthetase [Benincasa hispida]	[more]

BLAST of Moc10g00850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	2.3e-160	61.76	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	2.3e-43	32.58	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
P15925	9.1e-32	27.93	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]
D4GW05	7.7e-31	30.21	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]
Q9HS44	8.5e-30	30.66	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]

BLAST of Moc10g00850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJG1	1.7e-307	100.00	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]
A0A6J1E819	6.5e-275	88.50	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A1S3CNR7	1.1e-269	88.32	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A0A0L7A4	1.0e-267	87.59	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A6J1J6Z7	1.7e-262	86.31	dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 ...	[more]

BLAST of Moc10g00850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	1.6e-161	61.76	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	5.3e-27	31.06	DHFS-FPGS homolog D	[more]
AT3G55630.2	1.5e-26	31.06	DHFS-FPGS homolog D	[more]
AT5G05980.1	5.5e-24	40.99	DHFS-FPGS homolog B	[more]
AT5G05980.2	5.5e-24	40.99	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 38..368 e-value: 5.5E-85 score: 286.9
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..368
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 103..254 e-value: 3.2E-8 score: 33.7
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 81..544 e-value: 1.2E-89 score: 299.4
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..546
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 381..548 e-value: 8.2E-17 score: 63.7
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 385..546
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..546
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 201..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g00850.1	Moc10g00850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006761	dihydrofolate biosynthetic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008841	dihydrofolate synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity