Homology
BLAST of Moc09g41980 vs. NCBI nr
Match:
XP_022151863.1 (probable polygalacturonase isoform X3 [Momordica charantia])
HSP 1 Score: 969.9 bits (2506), Expect = 7.7e-279
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK 60
MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK
Sbjct: 1 MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK 60
Query: 61 AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV 120
AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV
Sbjct: 61 AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV 120
Query: 121 KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH 180
KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH
Sbjct: 121 KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH 180
Query: 181 LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV 240
LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV
Sbjct: 181 LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV 240
Query: 241 CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS 300
CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS
Sbjct: 241 CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS 300
Query: 301 EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD 360
EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD
Sbjct: 301 EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD 360
Query: 361 PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF 420
PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF
Sbjct: 361 PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF 420
Query: 421 SDLVSPEICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 466
SDLVSPEICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR
Sbjct: 421 SDLVSPEICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 465
BLAST of Moc09g41980 vs. NCBI nr
Match:
XP_022151862.1 (probable polygalacturonase isoform X2 [Momordica charantia])
HSP 1 Score: 957.2 bits (2473), Expect = 5.1e-275
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0
Query: 7 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 66
LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI
Sbjct: 20 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 79
Query: 67 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 126
FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY
Sbjct: 80 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 139
Query: 127 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 186
GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN
Sbjct: 140 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 199
Query: 187 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 246
STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY
Sbjct: 200 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 259
Query: 247 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 306
ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN
Sbjct: 260 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 319
Query: 307 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 366
LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT
Sbjct: 320 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 379
Query: 367 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 426
VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP
Sbjct: 380 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 439
Query: 427 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 466
EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR
Sbjct: 440 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 478
BLAST of Moc09g41980 vs. NCBI nr
Match:
XP_022151861.1 (probable polygalacturonase isoform X1 [Momordica charantia])
HSP 1 Score: 957.2 bits (2473), Expect = 5.1e-275
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0
Query: 7 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 66
LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI
Sbjct: 20 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 79
Query: 67 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 126
FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY
Sbjct: 80 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 139
Query: 127 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 186
GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN
Sbjct: 140 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 199
Query: 187 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 246
STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY
Sbjct: 200 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 259
Query: 247 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 306
ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN
Sbjct: 260 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 319
Query: 307 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 366
LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT
Sbjct: 320 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 379
Query: 367 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 426
VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP
Sbjct: 380 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 439
Query: 427 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 466
EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR
Sbjct: 440 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 478
BLAST of Moc09g41980 vs. NCBI nr
Match:
XP_022967082.1 (probable polygalacturonase isoform X2 [Cucurbita maxima])
HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 393/455 (86.37%), Postives = 425/455 (93.41%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIA-RSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNT 60
MKTP TLVDVLLLLALFC++P +A SSS+CKL N EIRPHSVSITDFGAVGDG+TLNT
Sbjct: 1 MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
Query: 61 KAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPV 120
KAFQNAIFYLNSFADKGGAKL VPAGRWLTGSFDLISHLTLWLDKDAVILGSTIS+DWP+
Sbjct: 61 KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
Query: 121 VKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRP 180
+KPLPSYGRGRELPG+RHRSLIYGCNLT+V+ITGENGTIDGQGSIWWDWFH KTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
Query: 181 HLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAE 240
HLVE+MNST VVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAP DSPNTDGIDPDSS+E
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
Query: 241 VCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGI 300
VCIEDCYISTGDDLISIKSGWD+YG+EYA PSTNITIRRLTGQT TSAG+AIGSEMSGG+
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 300
Query: 301 SEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCY 360
SEI VEN+LFFNS MGIRIKTSPGR GYVRNVYISNVSL +V++AIRFTG YGEHPDS Y
Sbjct: 301 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGY 360
Query: 361 DPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQG 420
+P ALPTVE ITIKHVSGENI+TAG+LEGIEGDDFHNICL+NITLNV+SMH WNCSHVQG
Sbjct: 361 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQG 420
Query: 421 FSDLVSPEICQPLKEKISPRHILECYLPPNFLWNS 455
FSD VSP+ C L+E+I P+HI +CYLPPNF+ S
Sbjct: 421 FSDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQS 454
BLAST of Moc09g41980 vs. NCBI nr
Match:
XP_022967081.1 (probable polygalacturonase isoform X1 [Cucurbita maxima])
HSP 1 Score: 829.3 bits (2141), Expect = 1.6e-236
Identity = 392/452 (86.73%), Postives = 424/452 (93.81%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIA-RSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNT 60
MKTP TLVDVLLLLALFC++P +A SSS+CKL N EIRPHSVSITDFGAVGDG+TLNT
Sbjct: 1 MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
Query: 61 KAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPV 120
KAFQNAIFYLNSFADKGGAKL VPAGRWLTGSFDLISHLTLWLDKDAVILGSTIS+DWP+
Sbjct: 61 KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
Query: 121 VKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRP 180
+KPLPSYGRGRELPG+RHRSLIYGCNLT+V+ITGENGTIDGQGSIWWDWFH KTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
Query: 181 HLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAE 240
HLVE+MNST VVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAP DSPNTDGIDPDSS+E
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
Query: 241 VCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGI 300
VCIEDCYISTGDDLISIKSGWD+YG+EYA PSTNITIRRLTGQT TSAG+AIGSEMSGG+
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 300
Query: 301 SEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCY 360
SEI VEN+LFFNS MGIRIKTSPGR GYVRNVYISNVSL +V++AIRFTG YGEHPDS Y
Sbjct: 301 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGY 360
Query: 361 DPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQG 420
+P ALPTVE ITIKHVSGENI+TAG+LEGIEGDDFHNICL+NITLNV+SMH WNCSHVQG
Sbjct: 361 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQG 420
Query: 421 FSDLVSPEICQPLKEKISPRHILECYLPPNFL 452
FSD VSP+ C L+E+I P+HI +CYLPPNF+
Sbjct: 421 FSDFVSPKTCHSLRERIFPQHISDCYLPPNFI 451
BLAST of Moc09g41980 vs. ExPASy Swiss-Prot
Match:
A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)
HSP 1 Score: 463.8 bits (1192), Expect = 2.3e-129
Identity = 226/451 (50.11%), Postives = 299/451 (66.30%), Query Frame = 0
Query: 7 LVDVLLLLALF---CLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQ 66
LV V+LLL++ C RI + + + R HS S+ DFG VGDG TLNTKAFQ
Sbjct: 28 LVAVVLLLSVSRGECRKGRILEALEY----SAISCRAHSASLVDFGGVGDGQTLNTKAFQ 87
Query: 67 NAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPL 126
+A+ L+ + +GGA+L VPAG+WLTGSF L SH TL+L +DAV+L S + WPV+KPL
Sbjct: 88 DAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIKPL 147
Query: 127 PSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVE 186
PSYGRGR+ R+ SLI+G NLTDVIITG+NGTIDGQG +WW FH L YTRP+L+E
Sbjct: 148 PSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIE 207
Query: 187 LMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIE 246
LM S + ISNLT NSP W +HPVY +L+Q +TILAP+ SPNTDGI+PDS IE
Sbjct: 208 LMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRIE 267
Query: 247 DCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIH 306
DCYI +GDD +++KSGWD+YGI Y P+ + IRRLT + SA +A+GSEMSGGI ++
Sbjct: 268 DCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVR 327
Query: 307 VENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMA 366
E+++ NS GIRIKT GR GYV+++Y+ +++ + A TGNYG H D+ YDP A
Sbjct: 328 AEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKA 387
Query: 367 LPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHH---WNCSHVQGF 426
P ++GI + + EN+ A LEGI D F IC+SN+T+++ + W C+ V+G
Sbjct: 388 FPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGI 447
Query: 427 SDLVSPEICQPLKEKISPRHILECYLPPNFL 452
S V+P C L ++ P C P L
Sbjct: 448 SSGVTPTPCSTLPDQ-GPEKTSLCNFPAESL 473
BLAST of Moc09g41980 vs. ExPASy Swiss-Prot
Match:
Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)
HSP 1 Score: 142.9 bits (359), Expect = 9.1e-33
Identity = 131/452 (28.98%), Postives = 198/452 (43.81%), Query Frame = 0
Query: 28 SFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAIFYLNSFADKGGAKLLVPAGRW 87
S KL++ ++ +GAVGDG +T+AF N+ K A LLVPA +
Sbjct: 44 SLKKLVHSRHDAATVFNVEQYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKK 103
Query: 88 -----LTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSYGRGRELPGKRHRSLIY 147
L HL+ +D TI V +P P+ K + +
Sbjct: 104 FFVNNLVFRGPCQPHLSFKVD-------GTI-----VAQPDPA-------RWKNSKIWLQ 163
Query: 148 GCNLTDVIITGENGTIDGQGSIWW----DWFHRKTL--NYTRPHLVELMNSTRVVISNLT 207
LTD + G G IDGQG WW + +T+ + RP +++ S V + LT
Sbjct: 164 FAQLTDFNLMG-TGVIDGQGQQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELT 223
Query: 208 FKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCYISTGDDLISI 267
NSP + L C V +Q + I AP DSPNTDGID +S IE C I TGDD I+I
Sbjct: 224 LMNSPEFHLVFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAI 283
Query: 268 KSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGS----EMSGGISEIHVENLLFFNS 327
+G S+NITI+ L G++IGS +S +HV F ++
Sbjct: 284 GTG-----------SSNITIKDLI--CGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDT 343
Query: 328 RMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPTVEGITI 387
+ G+RIKT G G + NV + N + I Y +C + + ++G+T
Sbjct: 344 QNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTY 403
Query: 388 KHVSGENIETAGL-LEGIEGDDFHNICLSNITLNVTSMHHWNC--SHVQGFSDLVSPEIC 447
K++ G + A + L + I LSN++L +TS +C + +GF C
Sbjct: 404 KNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPASCVDKNARGFYSGRLIPTC 455
Query: 448 QPLKEKISPRHILECYLPPNFLWNSGEKNRGA 462
+ L+ SP+ P + ++N+GA
Sbjct: 464 KNLRPGPSPKEFELQQQPTTVM----DENKGA 455
BLAST of Moc09g41980 vs. ExPASy Swiss-Prot
Match:
Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)
HSP 1 Score: 140.6 bits (353), Expect = 4.5e-32
Identity = 122/430 (28.37%), Postives = 188/430 (43.72%), Query Frame = 0
Query: 44 SITDFGAVGDGITLNTKAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLW-- 103
++ +GAVGDG +T AF+ N+ +K A LVPA + ++++L +
Sbjct: 61 NVEHYGAVGDGKHDSTDAFEKT---WNAACNKLSAVFLVPANKKF-----VVNNLVFYGP 120
Query: 104 ------LDKDAVILGSTISNDWPVVKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGEN 163
D I W K + ++ LTD + G
Sbjct: 121 CQPHFSFKVDGTIAAYPDPAKW-----------------KNSKIWMHFARLTDFNLMG-T 180
Query: 164 GTIDGQGSIWWDWFHRKTLN-------YTRPHLVELMNSTRVVISNLTFKNSPFWTLHPV 223
G IDGQG+ WW KT+N RP +++ S V + LT NSP + L
Sbjct: 181 GVIDGQGNRWWS-DQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFG 240
Query: 224 YCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCYISTGDDLISIKSGWDKYGIEYA 283
C V +Q + I AP DSPNTDGID +S IE C I TGDD +++ +G
Sbjct: 241 ECDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG--------- 300
Query: 284 HPSTNITIRRLTGQTRTSAGVAIGSEMSGG----ISEIHVENLLFFNSRMGIRIKTSPGR 343
S+NITI+ LT G++IGS G +S +H++ F +++ G+RIKT G
Sbjct: 301 --SSNITIKDLT--CGPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGG 360
Query: 344 RGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPTVEGITIKHVSGENIETAG 403
G ++ NV + N + I Y +C + + ++ +T K++ G + TA
Sbjct: 361 SGLASHITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAA 420
Query: 404 L-LEGIEGDDFHNICLSNITLNVTSMHHWNC--SHVQGFSDLVSPEICQPLKEKISPRHI 452
+ L + NI LSN+ L +TS C + G+ C+ L +P+
Sbjct: 421 IQLMCSDSVPCSNIKLSNVFLKLTSGKVATCVNKNANGYYTNPLNPSCKSLHPGRTPKE- 449
BLAST of Moc09g41980 vs. ExPASy Swiss-Prot
Match:
Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)
HSP 1 Score: 122.9 bits (307), Expect = 9.8e-27
Identity = 97/312 (31.09%), Postives = 157/312 (50.32%), Query Frame = 0
Query: 41 HSVSITDFGAVGDGITLNTKAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTL 100
+++ +T FGAVGDG+T +++AF A + + G + +VPAG +T
Sbjct: 22 NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG------------MTF 81
Query: 101 WLDKDAVILGSTISNDWPVVKPL------PSYGRGRELPGKRHRSLIYGCNLTDVIITGE 160
L + GS S PV + PS G + G + + +++ ++ ++I G+
Sbjct: 82 ML-QPLKFQGSCKST--PVFVQMLGKLVAPSKGNWK---GDKDQWILF-TDIEGLVIEGD 141
Query: 161 NGTIDGQGSIWWDWFHRKTLNYTRPHLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVL 220
G I+GQGS WW+ H+ +RP ++ + + +S LT +SP +H C+ V
Sbjct: 142 -GEINGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVT 201
Query: 221 VQNVTILAPLDSPNTDGIDPDSSAEVCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNI 280
+ ++ I AP SPNTDGID +S+ V I+DC I+TGDD I+I SG ++NI
Sbjct: 202 ISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNI 261
Query: 281 TIRRLTGQTRTSAGVAIGSEMSGG----ISEIHVENLLFFNSRMGIRIKTSPGRRGYVRN 340
I + G++IGS G + + V+N F + G RIKT G GY R
Sbjct: 262 HISGI--DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARM 292
Query: 341 VYISNVSLFNVD 343
+ + ++L NV+
Sbjct: 322 ITFNGITLDNVE 292
BLAST of Moc09g41980 vs. ExPASy Swiss-Prot
Match:
Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)
HSP 1 Score: 122.1 bits (305), Expect = 1.7e-26
Identity = 97/348 (27.87%), Postives = 150/348 (43.10%), Query Frame = 0
Query: 40 PHSVSITDFGAVGDGITLNTKAFQNAIFYLNSFADKGGAKLLVPAGR-WLTGSFDLIS-- 99
P +VS++DFGA GDG T +T+AF NA + + + G LLVP G +L S L
Sbjct: 65 PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124
Query: 100 HLTLWLDKDAVILGSTISNDWPVVKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENG 159
+ L + + S +D+ + + L + G+ G
Sbjct: 125 NSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLS----------------VDGGDTG 184
Query: 160 TIDGQGSIWW--DWFHRKTLNYTR-PHLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKV 219
+DG G WW K T+ P + NS +++ NL +N+ + CS V
Sbjct: 185 VVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNV 244
Query: 220 LVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTN 279
V NV + AP DSPNTDGI ++ + + + I TGDD ISI+SG S N
Sbjct: 245 QVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQN 304
Query: 280 ITIRRLTGQTRTSAGVAIGS----EMSGGISEIHVENLLFFNSRMGIRIKTSPGRRGYVR 339
+ I +T G++IGS +S + V+ + G+RIKT G G
Sbjct: 305 VQINDIT--CGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTAS 364
Query: 340 NVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPTVEGITIKHVSG 378
N+ N+ + NV I +Y + C + V+ + + +SG
Sbjct: 365 NIIFQNIQMDNVKNPIIIDQDYCD-KSKCTTEKSAVQVKNVVYRDISG 380
BLAST of Moc09g41980 vs. ExPASy TrEMBL
Match:
A0A6J1DDC5 (probable polygalacturonase isoform X3 OS=Momordica charantia OX=3673 GN=LOC111019738 PE=3 SV=1)
HSP 1 Score: 969.9 bits (2506), Expect = 3.7e-279
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK 60
MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK
Sbjct: 1 MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK 60
Query: 61 AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV 120
AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV
Sbjct: 61 AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV 120
Query: 121 KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH 180
KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH
Sbjct: 121 KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH 180
Query: 181 LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV 240
LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV
Sbjct: 181 LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV 240
Query: 241 CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS 300
CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS
Sbjct: 241 CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS 300
Query: 301 EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD 360
EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD
Sbjct: 301 EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD 360
Query: 361 PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF 420
PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF
Sbjct: 361 PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF 420
Query: 421 SDLVSPEICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 466
SDLVSPEICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR
Sbjct: 421 SDLVSPEICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 465
BLAST of Moc09g41980 vs. ExPASy TrEMBL
Match:
A0A6J1DFX6 (probable polygalacturonase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019738 PE=3 SV=1)
HSP 1 Score: 957.2 bits (2473), Expect = 2.5e-275
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0
Query: 7 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 66
LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI
Sbjct: 20 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 79
Query: 67 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 126
FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY
Sbjct: 80 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 139
Query: 127 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 186
GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN
Sbjct: 140 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 199
Query: 187 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 246
STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY
Sbjct: 200 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 259
Query: 247 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 306
ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN
Sbjct: 260 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 319
Query: 307 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 366
LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT
Sbjct: 320 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 379
Query: 367 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 426
VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP
Sbjct: 380 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 439
Query: 427 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 466
EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR
Sbjct: 440 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 478
BLAST of Moc09g41980 vs. ExPASy TrEMBL
Match:
A0A6J1DEM1 (probable polygalacturonase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019738 PE=3 SV=1)
HSP 1 Score: 957.2 bits (2473), Expect = 2.5e-275
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0
Query: 7 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 66
LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI
Sbjct: 20 LVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAI 79
Query: 67 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 126
FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY
Sbjct: 80 FYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSY 139
Query: 127 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 186
GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN
Sbjct: 140 GRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMN 199
Query: 187 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 246
STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY
Sbjct: 200 STRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCY 259
Query: 247 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 306
ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN
Sbjct: 260 ISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVEN 319
Query: 307 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 366
LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT
Sbjct: 320 LLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPT 379
Query: 367 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 426
VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP
Sbjct: 380 VEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDLVSP 439
Query: 427 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 466
EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR
Sbjct: 440 EICQPLKEKISPRHILECYLPPNFLWNSGEKNRGAGLLR 478
BLAST of Moc09g41980 vs. ExPASy TrEMBL
Match:
A0A6J1HPS3 (probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466589 PE=3 SV=1)
HSP 1 Score: 829.7 bits (2142), Expect = 6.0e-237
Identity = 393/455 (86.37%), Postives = 425/455 (93.41%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIA-RSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNT 60
MKTP TLVDVLLLLALFC++P +A SSS+CKL N EIRPHSVSITDFGAVGDG+TLNT
Sbjct: 1 MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
Query: 61 KAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPV 120
KAFQNAIFYLNSFADKGGAKL VPAGRWLTGSFDLISHLTLWLDKDAVILGSTIS+DWP+
Sbjct: 61 KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
Query: 121 VKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRP 180
+KPLPSYGRGRELPG+RHRSLIYGCNLT+V+ITGENGTIDGQGSIWWDWFH KTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
Query: 181 HLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAE 240
HLVE+MNST VVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAP DSPNTDGIDPDSS+E
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
Query: 241 VCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGI 300
VCIEDCYISTGDDLISIKSGWD+YG+EYA PSTNITIRRLTGQT TSAG+AIGSEMSGG+
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 300
Query: 301 SEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCY 360
SEI VEN+LFFNS MGIRIKTSPGR GYVRNVYISNVSL +V++AIRFTG YGEHPDS Y
Sbjct: 301 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGY 360
Query: 361 DPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQG 420
+P ALPTVE ITIKHVSGENI+TAG+LEGIEGDDFHNICL+NITLNV+SMH WNCSHVQG
Sbjct: 361 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQG 420
Query: 421 FSDLVSPEICQPLKEKISPRHILECYLPPNFLWNS 455
FSD VSP+ C L+E+I P+HI +CYLPPNF+ S
Sbjct: 421 FSDFVSPKTCHSLRERIFPQHISDCYLPPNFIAQS 454
BLAST of Moc09g41980 vs. ExPASy TrEMBL
Match:
A0A6J1HR20 (probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466589 PE=3 SV=1)
HSP 1 Score: 829.3 bits (2141), Expect = 7.8e-237
Identity = 392/452 (86.73%), Postives = 424/452 (93.81%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIA-RSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNT 60
MKTP TLVDVLLLLALFC++P +A SSS+CKL N EIRPHSVSITDFGAVGDG+TLNT
Sbjct: 1 MKTPLTLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 60
Query: 61 KAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPV 120
KAFQNAIFYLNSFADKGGAKL VPAGRWLTGSFDLISHLTLWLDKDAVILGSTIS+DWP+
Sbjct: 61 KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 120
Query: 121 VKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRP 180
+KPLPSYGRGRELPG+RHRSLIYGCNLT+V+ITGENGTIDGQGSIWWDWFH KTLNYTRP
Sbjct: 121 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 180
Query: 181 HLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAE 240
HLVE+MNST VVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAP DSPNTDGIDPDSS+E
Sbjct: 181 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 240
Query: 241 VCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGI 300
VCIEDCYISTGDDLISIKSGWD+YG+EYA PSTNITIRRLTGQT TSAG+AIGSEMSGG+
Sbjct: 241 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 300
Query: 301 SEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCY 360
SEI VEN+LFFNS MGIRIKTSPGR GYVRNVYISNVSL +V++AIRFTG YGEHPDS Y
Sbjct: 301 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGY 360
Query: 361 DPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQG 420
+P ALPTVE ITIKHVSGENI+TAG+LEGIEGDDFHNICL+NITLNV+SMH WNCSHVQG
Sbjct: 361 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQG 420
Query: 421 FSDLVSPEICQPLKEKISPRHILECYLPPNFL 452
FSD VSP+ C L+E+I P+HI +CYLPPNF+
Sbjct: 421 FSDFVSPKTCHSLRERIFPQHISDCYLPPNFI 451
BLAST of Moc09g41980 vs. TAIR 10
Match:
AT3G62110.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 643.3 bits (1658), Expect = 1.5e-184
Identity = 312/450 (69.33%), Postives = 361/450 (80.22%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEI-RPHSVSITDFGAVGDGITLNT 60
MK F L+ VLL+ A + + S C+ N+A + RPHSVSIT+FGAVGDG+TLNT
Sbjct: 1 MKRSFLLLYVLLVQAFYGAWCSVG-ESLHCEYSNLASLHRPHSVSITEFGAVGDGVTLNT 60
Query: 61 KAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPV 120
KAFQNA+FYLNSF+DKGGAKL VPAG+WLTGSFDLISHLTLWLDK A ILGST S +WPV
Sbjct: 61 KAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWLDKGATILGSTSSENWPV 120
Query: 121 VKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRP 180
V PLPSYGRGRELPG+RHRSLIYG NLTDV+ITGENGTIDGQG++WWDWF LNYTRP
Sbjct: 121 VDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQGTVWWDWFRNGELNYTRP 180
Query: 181 HLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAE 240
HLVELMNST ++ISNLTF NSPFW +HPVYC V+V+N+TILAPL+SPNTDG+DPDSS
Sbjct: 181 HLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILAPLESPNTDGVDPDSSTN 240
Query: 241 VCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGI 300
VCIEDCYI TGDDL+SIKSGWD+YGI YA PS+ I I RLTGQT +S+G+AIGSEMSGG+
Sbjct: 241 VCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGGV 300
Query: 301 SEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCY 360
SEI++++L FNS GIRIKTS GR GYVRNV+I NV L NV AIRFTG YGEHPD Y
Sbjct: 301 SEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKY 360
Query: 361 DPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNV---TSMHHWNCSH 420
DP ALP +E IT ++V+G+ I AGLLEGIEGD F NIC N+TL V + W CS+
Sbjct: 361 DPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECSN 420
Query: 421 VQGFSDLVSPEI-CQPLKEKISPRHILECY 446
V+G+S VSPEI C LKE I P H +C+
Sbjct: 421 VRGYSQWVSPEITCDSLKESIFPEHGSDCF 449
BLAST of Moc09g41980 vs. TAIR 10
Match:
AT4G33440.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 472.2 bits (1214), Expect = 4.7e-133
Identity = 220/394 (55.84%), Postives = 290/394 (73.60%), Query Frame = 0
Query: 43 VSITDFGAVGDGITLNTKAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWL 102
+SITDFG VGDG T NT AF+ A+ +L FA +GGA+L VP G WL+GSF+L S+ TL+L
Sbjct: 72 MSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFL 131
Query: 103 DKDAVILGSTISNDWPVVKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQG 162
++ A+ILGS ++WP+++PLPSYGRGRE PG RH SLI+G NLT+V+ITGENGTIDGQG
Sbjct: 132 ERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQG 191
Query: 163 SIWWDWFHRKTLNYTRPHLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILA 222
+WW+ + +TL +TR HL+EL NS ++ISNLT NSPFWT+HPVYCS V+++N+TILA
Sbjct: 192 KMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTILA 251
Query: 223 PLDSPNTDGIDPDSSAEVCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQ 282
P+++PNTDGIDPDSS VCIEDCYI +GDDL+++KSGWD+YG+ A PS+NI IRR++G
Sbjct: 252 PMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPSSNIVIRRISGT 311
Query: 283 TRTSAGVAIGSEMSGGISEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVD 342
TRT +GV IGSEMSGGI I VE++ ++S G+RIKT GR GY+ N+ +NV L V
Sbjct: 312 TRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNITFNNVLLEKVK 371
Query: 343 IAIRFTGNYGEHPDSCYDPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNI 402
+ IRF+ +H D +DP ALP V+GI I +V N A +L G+EG F ++CL N+
Sbjct: 372 VPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLLGVEGTSFQDVCLRNV 431
Query: 403 T-LNVTSMHHWNCSHVQGFSDLVSPEICQPLKEK 436
T L + W C V G++ V P C L +K
Sbjct: 432 TLLGLPKTEKWKCKDVSGYASDVFPLSCPQLLQK 465
BLAST of Moc09g41980 vs. TAIR 10
Match:
AT3G16850.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 465.3 bits (1196), Expect = 5.7e-131
Identity = 233/432 (53.94%), Postives = 299/432 (69.21%), Query Frame = 0
Query: 1 MKTPFTLVDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTK 60
MK P LV LLA L +++ CK + RPHSVSI +FGAVGDG TLNT
Sbjct: 1 MKMPVALV---WLLAFTILLISGEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTI 60
Query: 61 AFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVV 120
AFQNA+FYL SFADKGGA+L VP G+WLTGSF+L SHLTL+L+K A IL S + W VV
Sbjct: 61 AFQNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVV 120
Query: 121 KPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPH 180
PLPSYGRG ELPGKR+RSLI G NL DV+ITGENGT DGQG+ WW+W +LNY+RPH
Sbjct: 121 SPLPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPH 180
Query: 181 LVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEV 240
++E ++S ++ISNLTF N+P +HPVYCS++ ++ V I +DSP+ G+ PDSS V
Sbjct: 181 IIEFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNV 240
Query: 241 CIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGIS 300
CIED I+ G D +S+KSGWD+YGI Y P+T + IR L ++ T AG++ GSEMSGG+S
Sbjct: 241 CIEDSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVS 300
Query: 301 EIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYD 360
++ VE L +S +G+ +T+ GR GY+RN+ IS+V L +VD AI G+ G HPD +D
Sbjct: 301 DVTVERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFD 360
Query: 361 PMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGF 420
ALP V I +++ +G +I AG L GI F +ICL++I L S W CS+V GF
Sbjct: 361 RDALPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADIHLQTRSEESWICSNVSGF 420
Query: 421 SDLVSPEICQPL 433
SD VSPE CQ L
Sbjct: 421 SDDVSPEPCQEL 429
BLAST of Moc09g41980 vs. TAIR 10
Match:
AT4G23820.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 461.8 bits (1187), Expect = 6.3e-130
Identity = 225/428 (52.57%), Postives = 294/428 (68.69%), Query Frame = 0
Query: 8 VDVLLLLALFCLAPRIARSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQNAIF 67
V + L LF + + S + C I R +SITDFG VGDG T+NTKAF+ AI+
Sbjct: 6 VSIFLFSCLFVSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAAIY 65
Query: 68 YLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPLPSYG 127
+ +GG L +P G +LT SF+L SH+TL+L K AVI + +WP++ PLPSYG
Sbjct: 66 RIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYG 125
Query: 128 RGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVELMNS 187
RGRELPG R+ S I+G L DV+ITG+NGTIDGQG +WW+ + +TL YTRP+L+E +S
Sbjct: 126 RGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDS 185
Query: 188 TRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIEDCYI 247
++ISN+ F+NSPFW +HPVYCS V++ +VTILAP DSPNTDGIDPDSS VCIED YI
Sbjct: 186 KEIIISNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYI 245
Query: 248 STGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIHVENL 307
STGDDL++IKSGWD+YGI Y PS+NITIRR+TG + AG+AIGSE SGGI I E++
Sbjct: 246 STGDDLVAIKSGWDQYGIAYGRPSSNITIRRITGSS-PFAGIAIGSETSGGIKNIIAEHI 305
Query: 308 LFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMALPTV 367
N +G+ IKT+ GR GY++N+ IS+V + I+ G+ G+HPD Y+P ALP V
Sbjct: 306 TLSNMGVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVV 365
Query: 368 EGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITL--NVTSMHHWNCSHVQGFSDLVS 427
+GI IK+V G N+ AG ++G++G F ICLS I L ++ S W CS V G S VS
Sbjct: 366 KGIHIKNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVS 425
Query: 428 PEICQPLK 434
P C L+
Sbjct: 426 PWPCSELR 432
BLAST of Moc09g41980 vs. TAIR 10
Match:
AT4G23500.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 459.9 bits (1182), Expect = 2.4e-129
Identity = 215/412 (52.18%), Postives = 287/412 (69.66%), Query Frame = 0
Query: 39 RPHSVSITDFGAVGDGITLNTKAFQNAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHL 98
R +S S+ +FGAVGDG+T NT AF++A+ L+ FAD GG+ L VPAGRWLTG+F+L SH
Sbjct: 64 RAYSASLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRWLTGNFNLTSHF 123
Query: 99 TLWLDKDAVILGSTISNDWPVVKPLPSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTI 158
TL+L +DAVIL S +D+ V++PLPSYGRGR+ G R SL++G NLTDV+ITGENGTI
Sbjct: 124 TLFLHRDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLTDVVITGENGTI 183
Query: 159 DGQGSIWWDWFHRKTLNYTRPHLVELMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNV 218
DGQG WW F R L YTRP+L+E+M+S + ISNLTF NSP W +HPVY S + +Q +
Sbjct: 184 DGQGEPWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHPVYSSNIYIQGL 243
Query: 219 TILAPLDSPNTDGIDPDSSAEVCIEDCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRR 278
TILAP+ PNTDGI+PDS IEDCYI +GDD I++KSGWD+YGI Y P+ + IRR
Sbjct: 244 TILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKSGWDQYGINYGMPTKQLLIRR 303
Query: 279 LTGQTRTSAGVAIGSEMSGGISEIHVENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSL 338
LT + SA +A+GSEMSGGI ++ E+++ NS GIRIKT+ GR GYV++VY+ +++
Sbjct: 304 LTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRIKTAIGRGGYVKDVYVRGMTM 363
Query: 339 FNVDIAIRFTGNYGEHPDSCYDPMALPTVEGITIKHVSGENIETAGLLEGIEGDDFHNIC 398
+ TG+YG HPD YDP ALP ++ I + + EN+ L GI GD F IC
Sbjct: 364 MTMKYVFWMTGSYGSHPDDHYDPKALPVIQNINYQDMVAENVTMPAQLAGISGDQFTGIC 423
Query: 399 LSNITLNVTSMHH---WNCSHVQGFSDLVSPEICQPLKEKISPRHILECYLP 448
+SN+T+ ++ WNC+ V G++ V+P+ CQ L EK P ++ C P
Sbjct: 424 ISNVTITLSKKPKKVLWNCTDVSGYTSGVTPQPCQLLPEK-QPGTVVPCNFP 474
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
A7PZL3 | 2.3e-129 | 50.11 | Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... | [more] |
Q7M1E7 | 9.1e-33 | 28.98 | Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1 | [more] |
Q9FY19 | 4.5e-32 | 28.37 | Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1 | [more] |
Q9LW07 | 9.8e-27 | 31.09 | Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... | [more] |
Q8RY29 | 1.7e-26 | 27.87 | Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DDC5 | 3.7e-279 | 100.00 | probable polygalacturonase isoform X3 OS=Momordica charantia OX=3673 GN=LOC11101... | [more] |
A0A6J1DFX6 | 2.5e-275 | 100.00 | probable polygalacturonase isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101... | [more] |
A0A6J1DEM1 | 2.5e-275 | 100.00 | probable polygalacturonase isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101... | [more] |
A0A6J1HPS3 | 6.0e-237 | 86.37 | probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146658... | [more] |
A0A6J1HR20 | 7.8e-237 | 86.73 | probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146658... | [more] |