Moc09g41010 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g41010
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncytochrome P450 71B34-like
Locationchr9: 31270687 .. 31272508 (-)
RNA-Seq ExpressionMoc09g41010
SyntenyMoc09g41010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCCTCCACCTCGGCTCCGTCCCGACCATCGTAATCTCCTCCGCCGCTGCCGCCCGAGACCTGTTTAAGCTCCACGACCTCGCGTCTTGCAGCCGCCCTCCCTTATCCGGCAGCGGAAGATTGTCCTACAATTTCCTCGACATAAGTTTCTCCCCGTATGGCGACCACTGGAGGGAGGTTCGAAAAATCTGCGTGCTTGAGCTGTTCAGCGTCAGGCGGGTGCAGTCGTTTCGGGGGGTTAGAGAACATGAGGTGGGTCTGCTTATAAACTCCATCGCTCAATCCTCAACCGCCGGAGCTCCGGTGGACGTGAGTGAGAAATCGTATTGTCTGACGGCGAGTATAATTACGCGGGTTGCTTTTGGGAAGAGCTTCAGAGGGGGTGAATTAGATAATGAGAAATTTGAAGAGGTTATCCGTAGAGGTTTGGTGGTAGTGGGAAGCTTCTCGGCGACTGACTTCTTCCCAAGCTTTGGTTGGATTATTGATTGGATGAATGGAGTTCGTGGGAGGTTGGAGAAGAGTTTTTCGGAGTTGGATTCTTTTTTTCAACATGTAATTGACCACCGCCTCAAATTTGGGGCGACTTGTGATGATGACAACAATATTTTGGATGTGTTGTTGAAGATGGAGAGAGAGCGCTCTGAATTTGATGCTCTGCAACTCTCTAGAGATTGCATCAAGGCACTTACCATGGTAAAAAGCTTCACTTTATAGCATACATGAGTTTTAGAACAGCTTTTTGTGCTCGGTTTGAAAATCAGATCAAAAGTTGAGAAATCCTTATTTTTCTTTAAAAAAAAAATTTAATTTTTGCATTTGTTTGACAAGGACAAGATTCATCTTTACCACCGAGGAATTTATCCCAACACTATGAAAACAACTCAGAGAAGTAGGAGAGAAAAAATAGAATAAATAGATGATAGTTTTCAAAGGGTAGTTAGGAGGATGATACTTGCAGGCACCTAAATGTTATGTTTAAAAATTTATACTCATTTTATATAAATACCTCAAAATTCAATCGGTCAAGACCAACTCACTACATCTTCTGTTCTTTACTAAATATATGTTTTTTTTTTTTTTTACTATAATATGCCGAGGTGGAGGATGAAAATTATCCTCTCTTTGAGGTATCAATAAAAACTAAAACATATATATAATTTTTTTATATATATTTATTAGTTTCGATGTGCGTCTTAATTAGGATATATTTCTAGCTGGAGTGGACACGGGAGCCGGAACAATTGTTTGGGCGATGGCAGAGCTGATTAAGAATCCAAGGGTTATGAAAAAGCTACAAGACGAGATCAGAAGCTCCATAAAAGAAGAAGATGGAATAGTGAAGGAGAGCCACCTCGAAAAACTCGAATATCTAAAAATGGTGGTCAAAGAAGTTCTGAGGCTTCATCCCCCGGGCCCACTCCTCCTTCCAAGAGAAACAATGTCCCATTTCAAGCTCGGCGGCTACGACATCGAACCCAAAGCCCATCTCCACGTCAATGTTTGGGCCATCGGGCGGGACCCGGAATCGTGGACCGACCCGGATGAGTTCCTGCCGGAGAGATTCGTGGGAAGCAACATCGATTTCAGAGGGCAGAATTTTGAGCTGTTGCCGTTCGGAGCTGGCCGGAGGAGCTGCCCGGGGATGCACATGGCCACCGTTGCGGTGGAGCTGGCGCTGGCTAATATGCTGCTGTGCTTTGATTGGAAATTGCCGCAAGGGATGAAAGAAGAAGATTTGGACGTGGAAGAGGATGCTGGTCTGACGGTCACCAAGAAATCGCCTCTTCATCTTATTCCTGTCGAATACTTCAAATAA

mRNA sequence

ATGCGCCTCCACCTCGGCTCCGTCCCGACCATCGTAATCTCCTCCGCCGCTGCCGCCCGAGACCTGTTTAAGCTCCACGACCTCGCGTCTTGCAGCCGCCCTCCCTTATCCGGCAGCGGAAGATTGTCCTACAATTTCCTCGACATAAGTTTCTCCCCGTATGGCGACCACTGGAGGGAGGTTCGAAAAATCTGCGTGCTTGAGCTGTTCAGCGTCAGGCGGGTGCAGTCGTTTCGGGGGGTTAGAGAACATGAGGTGGGTCTGCTTATAAACTCCATCGCTCAATCCTCAACCGCCGGAGCTCCGGTGGACGTGAGTGAGAAATCGTATTGTCTGACGGCGAGTATAATTACGCGGGTTGCTTTTGGGAAGAGCTTCAGAGGGGGTGAATTAGATAATGAGAAATTTGAAGAGGTTATCCGTAGAGGTTTGGTGGTAGTGGGAAGCTTCTCGGCGACTGACTTCTTCCCAAGCTTTGGTTGGATTATTGATTGGATGAATGGAGTTCGTGGGAGGTTGGAGAAGAGTTTTTCGGAGTTGGATTCTTTTTTTCAACATGTAATTGACCACCGCCTCAAATTTGGGGCGACTTGTGATGATGACAACAATATTTTGGATGTGTTGTTGAAGATGGAGAGAGAGCGCTCTGAATTTGATGCTCTGCAACTCTCTAGAGATTGCATCAAGGCACTTACCATGGATATATTTCTAGCTGGAGTGGACACGGGAGCCGGAACAATTGTTTGGGCGATGGCAGAGCTGATTAAGAATCCAAGGGTTATGAAAAAGCTACAAGACGAGATCAGAAGCTCCATAAAAGAAGAAGATGGAATAGTGAAGGAGAGCCACCTCGAAAAACTCGAATATCTAAAAATGGTGGTCAAAGAAGTTCTGAGGCTTCATCCCCCGGGCCCACTCCTCCTTCCAAGAGAAACAATGTCCCATTTCAAGCTCGGCGGCTACGACATCGAACCCAAAGCCCATCTCCACGTCAATGTTTGGGCCATCGGGCGGGACCCGGAATCGTGGACCGACCCGGATGAGTTCCTGCCGGAGAGATTCGTGGGAAGCAACATCGATTTCAGAGGGCAGAATTTTGAGCTGTTGCCGTTCGGAGCTGGCCGGAGGAGCTGCCCGGGGATGCACATGGCCACCGTTGCGGTGGAGCTGGCGCTGGCTAATATGCTGCTGTGCTTTGATTGGAAATTGCCGCAAGGGATGAAAGAAGAAGATTTGGACGTGGAAGAGGATGCTGGTCTGACGGTCACCAAGAAATCGCCTCTTCATCTTATTCCTGTCGAATACTTCAAATAA

Coding sequence (CDS)

ATGCGCCTCCACCTCGGCTCCGTCCCGACCATCGTAATCTCCTCCGCCGCTGCCGCCCGAGACCTGTTTAAGCTCCACGACCTCGCGTCTTGCAGCCGCCCTCCCTTATCCGGCAGCGGAAGATTGTCCTACAATTTCCTCGACATAAGTTTCTCCCCGTATGGCGACCACTGGAGGGAGGTTCGAAAAATCTGCGTGCTTGAGCTGTTCAGCGTCAGGCGGGTGCAGTCGTTTCGGGGGGTTAGAGAACATGAGGTGGGTCTGCTTATAAACTCCATCGCTCAATCCTCAACCGCCGGAGCTCCGGTGGACGTGAGTGAGAAATCGTATTGTCTGACGGCGAGTATAATTACGCGGGTTGCTTTTGGGAAGAGCTTCAGAGGGGGTGAATTAGATAATGAGAAATTTGAAGAGGTTATCCGTAGAGGTTTGGTGGTAGTGGGAAGCTTCTCGGCGACTGACTTCTTCCCAAGCTTTGGTTGGATTATTGATTGGATGAATGGAGTTCGTGGGAGGTTGGAGAAGAGTTTTTCGGAGTTGGATTCTTTTTTTCAACATGTAATTGACCACCGCCTCAAATTTGGGGCGACTTGTGATGATGACAACAATATTTTGGATGTGTTGTTGAAGATGGAGAGAGAGCGCTCTGAATTTGATGCTCTGCAACTCTCTAGAGATTGCATCAAGGCACTTACCATGGATATATTTCTAGCTGGAGTGGACACGGGAGCCGGAACAATTGTTTGGGCGATGGCAGAGCTGATTAAGAATCCAAGGGTTATGAAAAAGCTACAAGACGAGATCAGAAGCTCCATAAAAGAAGAAGATGGAATAGTGAAGGAGAGCCACCTCGAAAAACTCGAATATCTAAAAATGGTGGTCAAAGAAGTTCTGAGGCTTCATCCCCCGGGCCCACTCCTCCTTCCAAGAGAAACAATGTCCCATTTCAAGCTCGGCGGCTACGACATCGAACCCAAAGCCCATCTCCACGTCAATGTTTGGGCCATCGGGCGGGACCCGGAATCGTGGACCGACCCGGATGAGTTCCTGCCGGAGAGATTCGTGGGAAGCAACATCGATTTCAGAGGGCAGAATTTTGAGCTGTTGCCGTTCGGAGCTGGCCGGAGGAGCTGCCCGGGGATGCACATGGCCACCGTTGCGGTGGAGCTGGCGCTGGCTAATATGCTGCTGTGCTTTGATTGGAAATTGCCGCAAGGGATGAAAGAAGAAGATTTGGACGTGGAAGAGGATGCTGGTCTGACGGTCACCAAGAAATCGCCTCTTCATCTTATTCCTGTCGAATACTTCAAATAA

Protein sequence

MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWREVRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRVAFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSELDSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGVDTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRLHPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNIDFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGLTVTKKSPLHLIPVEYFK
Homology
BLAST of Moc09g41010 vs. NCBI nr
Match: XP_022151852.1 (cytochrome P450 71B34-like isoform X1 [Momordica charantia])

HSP 1 Score: 864.4 bits (2232), Expect = 4.3e-247
Identity = 424/437 (97.03%), Postives = 430/437 (98.40%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           MRLHLGSVPTI ISSAAAARD+FKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE
Sbjct: 75  MRLHLGSVPTIXISSAAAARDMFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 134

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSST+GA VDVSEKSY LTASIITRV
Sbjct: 135 VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTSGALVDVSEKSYSLTASIITRV 194

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFGKSFRGGELDNEKFEEVIRR LVV+GSFSATDFFPSFGWIIDWM+GVRGRLEKSF EL
Sbjct: 195 AFGKSFRGGELDNEKFEEVIRRALVVMGSFSATDFFPSFGWIIDWMSGVRGRLEKSFXEL 254

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIF+AGV
Sbjct: 255 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFIAGV 314

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DTGAGTIVWAM EL +NPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL
Sbjct: 315 DTGAGTIVWAMTELARNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 374

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID
Sbjct: 375 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 434

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL
Sbjct: 435 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 494

Query: 421 TVTKKSPLHLIPVEYFK 438
           TVTKKSPLHLIPVEYFK
Sbjct: 495 TVTKKSPLHLIPVEYFK 511

BLAST of Moc09g41010 vs. NCBI nr
Match: XP_022151853.1 (cytochrome P450 71B34-like isoform X2 [Momordica charantia])

HSP 1 Score: 855.5 bits (2209), Expect = 2.0e-244
Identity = 418/437 (95.65%), Postives = 428/437 (97.94%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           MRLHLGSVPTI ISSAAAARD+FKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE
Sbjct: 75  MRLHLGSVPTIXISSAAAARDMFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 134

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSST+GA VDVSEKSY LTASIITRV
Sbjct: 135 VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTSGALVDVSEKSYSLTASIITRV 194

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFGKSFRGGELDNEKFEEVIRR LVV+GSFSATDFFPSFGWIIDWM+GVRGRLEKSF EL
Sbjct: 195 AFGKSFRGGELDNEKFEEVIRRALVVMGSFSATDFFPSFGWIIDWMSGVRGRLEKSFLEL 254

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQL+RDCIKALTMDIF+AGV
Sbjct: 255 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLTRDCIKALTMDIFIAGV 314

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DTGAGTIVWAM EL +NPRVMKKLQDEIRSSIKEED +VKESHLEKLEYLKMVVKEVLRL
Sbjct: 315 DTGAGTIVWAMTELARNPRVMKKLQDEIRSSIKEEDRVVKESHLEKLEYLKMVVKEVLRL 374

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRETMSHFKLGGYDIEPKAHLHVN+WAIGRDPESWTDPDEFLPERFVGSNID
Sbjct: 375 HPPAPLLLPRETMSHFKLGGYDIEPKAHLHVNIWAIGRDPESWTDPDEFLPERFVGSNID 434

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLD+EEDAGL
Sbjct: 435 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDMEEDAGL 494

Query: 421 TVTKKSPLHLIPVEYFK 438
           TVTKKSPLHLIPVEYFK
Sbjct: 495 TVTKKSPLHLIPVEYFK 511

BLAST of Moc09g41010 vs. NCBI nr
Match: XP_022957287.1 (cytochrome P450 71B34-like [Cucurbita moschata])

HSP 1 Score: 668.3 bits (1723), Expect = 4.5e-188
Identity = 316/432 (73.15%), Postives = 375/432 (86.81%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPTIVISSAAAAR+LFK HDLASCSRP L+GSGRLSYN+LDISFSPYGDHWR 
Sbjct: 72  MLLQLGSVPTIVISSAAAARELFKFHDLASCSRPRLAGSGRLSYNYLDISFSPYGDHWRN 131

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKIC+LELF+ RRV+SFR +RE EVG L+NSI+QSS++ AP+D+SEKSY LTA+IITRV
Sbjct: 132 VRKICMLELFTSRRVESFREIREEEVGRLLNSISQSSSSSAPIDLSEKSYSLTANIITRV 191

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG  F GG+LD+E F+ V+   L  +GSFS TDF P+FGWIID +NGV GRLEKSF+E+
Sbjct: 192 AFGSIFSGGKLDDEHFQHVMHSALAAIGSFSMTDFIPTFGWIIDRLNGVHGRLEKSFAEM 251

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+FFQHV++ R+ F  +   + NI+DVLL+MERE SE DAL++++DC+KAL MDIFLAGV
Sbjct: 252 DAFFQHVVEDRINFKKSSKKEENIVDVLLRMERESSESDALKVTKDCVKALIMDIFLAGV 311

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           +TGAGTIVWAM EL++NPRVMKKLQ EIRS IKE  G VKE +LEKLEYLKMVVKEVLRL
Sbjct: 312 ETGAGTIVWAMTELVRNPRVMKKLQHEIRSCIKE--GSVKEINLEKLEYLKMVVKEVLRL 371

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRET+  FKL GYDI+PK+HLH+NVWAIGRDP+SWTDP+EF PERF+ SN+D
Sbjct: 372 HPPVPLLLPRETICPFKLNGYDIDPKSHLHINVWAIGRDPQSWTDPEEFFPERFIESNVD 431

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           ++GQNFEL+PFG GRR CPG++M T+ VELALAN+ LCFDWKLP GMKEED+D+EE+ GL
Sbjct: 432 YKGQNFELIPFGGGRRICPGINMGTLTVELALANLFLCFDWKLPNGMKEEDIDMEEEVGL 491

Query: 421 TVTKKSPLHLIP 433
           T+TKKSPL LIP
Sbjct: 492 TITKKSPLKLIP 501

BLAST of Moc09g41010 vs. NCBI nr
Match: XP_022956391.1 (cytochrome P450 71B26-like [Cucurbita moschata])

HSP 1 Score: 664.5 bits (1713), Expect = 6.5e-187
Identity = 316/432 (73.15%), Postives = 374/432 (86.57%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPTIVISSAAAAR+LFK HDLASCSRPPL  +GRLSYN+LD+S +PYG+HWR 
Sbjct: 78  MLLQLGSVPTIVISSAAAARELFKFHDLASCSRPPLHANGRLSYNYLDMSLTPYGEHWRN 137

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKIC+LELFS +RVQSF+ +RE EVG L+ SI+QSS++ +P+DVS+KSY LTA+IITRV
Sbjct: 138 VRKICMLELFSAQRVQSFQEIREEEVGRLVKSISQSSSSSSPIDVSDKSYSLTANIITRV 197

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG  F GG+LD+E F+ V+RR +  +GSFS TDFFP+FGWIID +NGV GRLEKSF  L
Sbjct: 198 AFGSIFSGGKLDDEHFQHVVRRAVAAIGSFSMTDFFPTFGWIIDRLNGVHGRLEKSFGVL 257

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+FFQ V+D+R+ F     ++ NI+DVLL+MERE SE DAL++++DCIKAL  DIFLAGV
Sbjct: 258 DAFFQSVVDNRINFKEISKNEENIVDVLLRMERESSESDALKVTQDCIKALIKDIFLAGV 317

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           +TGAGTIVWAMAEL++NPRVMKKLQDEIRS IKE+   VKE  LEKLEYLKMVVKEV+RL
Sbjct: 318 ETGAGTIVWAMAELVRNPRVMKKLQDEIRSGIKEDS--VKEIDLEKLEYLKMVVKEVMRL 377

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRET+S FKL GYDIEPKAHLH+NVWAIGRDP+ WT+P+EF PERF+GSNID
Sbjct: 378 HPPAPLLLPRETISPFKLNGYDIEPKAHLHINVWAIGRDPQYWTNPEEFFPERFIGSNID 437

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           ++GQNFELLPFG GRR CPG++MAT+ VELALAN+LLCFDWKLP  MKEED+D+EED GL
Sbjct: 438 YKGQNFELLPFGGGRRICPGINMATLTVELALANLLLCFDWKLPDEMKEEDVDMEEDVGL 497

Query: 421 TVTKKSPLHLIP 433
           TV +KSPL LIP
Sbjct: 498 TVAQKSPLQLIP 507

BLAST of Moc09g41010 vs. NCBI nr
Match: XP_023529060.1 (cytochrome P450 71B26-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.1 bits (1699), Expect = 2.7e-185
Identity = 315/433 (72.75%), Postives = 371/433 (85.68%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPT+VISSAAAAR+LFK HDLASCSRPPL  +GRLSYN+LD+S +PYG+HWR 
Sbjct: 77  MLLQLGSVPTVVISSAAAARELFKFHDLASCSRPPLHANGRLSYNYLDMSLAPYGEHWRN 136

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKIC+LELFS RRV+SF+ +RE EVG L+ SI+QSS++ +P+DVSEKSY LTA+IITRV
Sbjct: 137 VRKICMLELFSARRVESFQEIREEEVGRLVKSISQSSSSSSPIDVSEKSYSLTANIITRV 196

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG  F GG+LD+E F+ VIRR +  +GSFS TDFFP+FGWIID +NGV GRLEKSF  L
Sbjct: 197 AFGSIFSGGKLDDEHFQHVIRRAVTAIGSFSMTDFFPTFGWIIDRLNGVHGRLEKSFVVL 256

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+FFQ V+D+R+ F  +  ++ NI+DVLL+MERE SE DAL+++RDC KAL  DIF+AGV
Sbjct: 257 DAFFQSVVDNRINFKESSKNEQNIVDVLLRMERESSESDALKVTRDCTKALIKDIFMAGV 316

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           +TGAGTIVWAM EL++NPRVMKKLQDEIRS IKE+   VKE  LEKLEYLKMVVKEVLRL
Sbjct: 317 ETGAGTIVWAMTELVRNPRVMKKLQDEIRSCIKEDS--VKEVDLEKLEYLKMVVKEVLRL 376

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP  LLLPRET+S FKL GYDIEPKAHLH+NVWAIGRDP+SWT+P+EF PERF+GSNID
Sbjct: 377 HPPASLLLPRETISPFKLNGYDIEPKAHLHINVWAIGRDPKSWTNPEEFFPERFIGSNID 436

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           ++GQNFELLPFG GRR CPG++M T  VELALAN+LLCFDWKLP  +KEED D+EED G+
Sbjct: 437 YKGQNFELLPFGGGRRICPGINMGTFTVELALANLLLCFDWKLPNRIKEEDEDMEEDVGI 496

Query: 421 TVTKKSPLHLIPV 434
           TV KKSPL LIPV
Sbjct: 497 TVAKKSPLQLIPV 507

BLAST of Moc09g41010 vs. ExPASy Swiss-Prot
Match: Q9LIP4 (Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 3.4e-130
Identity = 218/435 (50.11%), Postives = 316/435 (72.64%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L  GS+PT+V+SS+  A+ + K+HDL  CSRP L+G   LSYN+LDI+FSP+ D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           +R+ICV ELFSV+RVQSF+ ++E EV  LI+S+++S++ G PV++SEK   LT  +  + 
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
            FG +F+G  L++++FE++I    + +GSFSA+D+FP+ GWIIDW+ G+ G+ E+S   L
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+F++ + D  L      +   + +D+LL++E+E +     +L+R+ IKA+ M++ + G+
Sbjct: 246 DAFYEQMFD--LHKQGNKEGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGI 305

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
            T A T+ WAM EL++NPRVMKK+Q EIR+ I ++  I  +  +++L YLKMV+ E  RL
Sbjct: 306 GTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDD-IDQLHYLKMVINETWRL 365

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP P L+PR+ MS F+L  Y I  K  L+VNVWAIGRDP++W DP+EFLPERFV S+ID
Sbjct: 366 HPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSID 425

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
            +GQ+FELLPFG+GRR CP M+M T  VE  LANML  FDWK+P GM  ED+D+EE  GL
Sbjct: 426 AKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPGL 485

Query: 421 TVTKKSPLHLIPVEY 436
             +KK+ L L+P++Y
Sbjct: 486 NASKKNELVLVPLKY 497

BLAST of Moc09g41010 vs. ExPASy Swiss-Prot
Match: Q9LIP6 (Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 5.8e-130
Identity = 224/436 (51.38%), Postives = 313/436 (71.79%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LG VPT+++SS+  A+   K+HDL  CSRP  +G+  LSYN+LDI+FSPY D+W+E
Sbjct: 66  MLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRK+ V ELFS ++V S + +++ EV  LI+SI++S+    P+++++    LT S++ R 
Sbjct: 126 VRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRT 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AF  +F G  L++E+F  ++R  L ++GSFSA+DF P  G IID + G++GR E+S  +L
Sbjct: 186 AFSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDL 245

Query: 181 DSFFQHVID-HRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAG 240
           D+F++ + D H+ K     +D    +D+LL++E+E +     +L+R+ IKA+ MD+ LAG
Sbjct: 246 DAFYEQMFDLHKQKKEEGSED---FVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAG 305

Query: 241 VDTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLR 300
           +DT A T+ WAMAEL KNPRVMKK+Q EIRS IK ++ I  +   +KLEYLKMV+KE  R
Sbjct: 306 MDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDD-TDKLEYLKMVIKETWR 365

Query: 301 LHPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNI 360
           LHP  PLL+PRE MS F++ GY I  K  LHVNVWAIGRDP++W DP+ FLPERF  +NI
Sbjct: 366 LHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNI 425

Query: 361 DFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAG 420
           D +GQ+FELLPFG GRR CP ++M T  VE  LAN+L  FDWKLP+GMK +D+D+EE  G
Sbjct: 426 DAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPG 485

Query: 421 LTVTKKSPLHLIPVEY 436
           LTV KK+ L L+P ++
Sbjct: 486 LTVNKKNELILVPTKF 497

BLAST of Moc09g41010 vs. ExPASy Swiss-Prot
Match: O64718 (Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 465.3 bits (1196), Expect = 7.6e-130
Identity = 225/436 (51.61%), Postives = 314/436 (72.02%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPT+V+SS+  A+ + K++DL  CSRP L+G+  LSYN+LDI+FSP+ D+W+E
Sbjct: 66  MLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           +R+ICV ELFS +RV S + ++E EV  LI S  +S++  +PV++SEK   LT S+I + 
Sbjct: 126 LRRICVQELFSAKRVHSIQPIKEEEVRKLIVSATESASQKSPVNLSEKFLDLTVSVICKA 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AF   F    L+N+ F+++I    + +GSFSA++FFP+ GWIIDW+ G++ R EKS  +L
Sbjct: 186 AFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDL 245

Query: 181 DSFFQHVID-HRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAG 240
           D F+Q + D H+ +     +D    +D+LLK+E+E +     +L+R+ +KA+ M++ L  
Sbjct: 246 DVFYQQMFDLHKQENKQGVED---FVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLGA 305

Query: 241 VDTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLR 300
           ++T A T+ WAMAELI+NPRVMKK+Q EIR+ +  +  ++    ++ L YLKMV+KE  R
Sbjct: 306 INTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINK-SVITLDDIDHLPYLKMVIKETWR 365

Query: 301 LHPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNI 360
           LHPP PLLLPRE MS F++ GY I+PK  L+VNVWAIGRDP+SW D D F PERF+ +NI
Sbjct: 366 LHPPVPLLLPREVMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNI 425

Query: 361 DFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAG 420
           D +GQNFELLPFG+GRR CPGM+M T  VE  LANML  FDW++P GM  ED+D+EE  G
Sbjct: 426 DAKGQNFELLPFGSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPG 485

Query: 421 LTVTKKSPLHLIPVEY 436
           L V KK+ L L+PV+Y
Sbjct: 486 LAVGKKNELLLVPVKY 497

BLAST of Moc09g41010 vs. ExPASy Swiss-Prot
Match: Q9LIP5 (Cytochrome P450 71B35 OS=Arabidopsis thaliana OX=3702 GN=CYP71B35 PE=2 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.3e-129
Identity = 223/432 (51.62%), Postives = 312/432 (72.22%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LG VPT+V+SS+  AR + ++HDL  C+RP LSG   LSYN+LDI+FSPY D+W+E
Sbjct: 65  MHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKE 124

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRK+CV ELFS ++V S + +++ EV  +I+SIA+S++   PV+++ K   LT S++ R 
Sbjct: 125 VRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRT 184

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG SF G  L++++F +++R  L ++GSFSA DF P  GWIID + G++GR E+S  +L
Sbjct: 185 AFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDL 244

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           ++FF+ + D  L      + + + +D+LL++E+E +     +L+R+ IKA+ +D+ LAG+
Sbjct: 245 NAFFEQMFD--LHKEGKKEGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGI 304

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DT A T+ WAM EL +NPRVMKK+Q EIR+ +     I  E  +++LEYLKMV+KE  RL
Sbjct: 305 DTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFED-MDQLEYLKMVIKETWRL 364

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HP  PLLLPRE MS F + GY I  K  LHVNVWAIGRDP++W DP+ FLPERF+ +NID
Sbjct: 365 HPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDNNID 424

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
            +GQ+FELLPFG GRR CP ++M T  VE  LAN+L  FDWKLP+G++ +D+DVEE  GL
Sbjct: 425 AKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEAPGL 484

Query: 421 TVTKKSPLHLIP 433
           TV KK+ L L+P
Sbjct: 485 TVNKKNELLLVP 493

BLAST of Moc09g41010 vs. ExPASy Swiss-Prot
Match: Q9LTL0 (Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.2e-129
Identity = 226/435 (51.95%), Postives = 313/435 (71.95%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LG VPT+++SS+  A+   + +DL  CSRP L+G   LSYN LD+S SPY ++W+E
Sbjct: 66  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           +RK+C  ELFS  ++QS + +++ EV  +I+SIA+SS+   PV++S+    LT S++ + 
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG SF G  L++++F +++R    ++GSFSA+DF P  GWIID  NG++G  +KSF +L
Sbjct: 186 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 245

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+F++ + D   +      +D  ++DVLL++E+E       +L+R+ IKA+ M+I L G+
Sbjct: 246 DAFYEQIFDLHKEEKEVGSED--LVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLGGI 305

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DT A T+ WAMAEL KNPRVMKK+Q EIR+ IK ++ I  +   +KLEYLKMV+KE  RL
Sbjct: 306 DTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDD-TDKLEYLKMVIKETWRL 365

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPR+ ++ F++ GY I  K  LHVNVWAIGRDP++W DP+ FLPERF  SNID
Sbjct: 366 HPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNID 425

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
            +GQNFELL FG+GRR CPG++M T  VE  LANML  FDWKLP+GM  ED+D+EE  GL
Sbjct: 426 AKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPGL 485

Query: 421 TVTKKSPLHLIPVEY 436
           TV+KKS L L+PV+Y
Sbjct: 486 TVSKKSELVLVPVKY 497

BLAST of Moc09g41010 vs. ExPASy TrEMBL
Match: A0A6J1DEL1 (cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019732 PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.1e-247
Identity = 424/437 (97.03%), Postives = 430/437 (98.40%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           MRLHLGSVPTI ISSAAAARD+FKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE
Sbjct: 75  MRLHLGSVPTIXISSAAAARDMFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 134

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSST+GA VDVSEKSY LTASIITRV
Sbjct: 135 VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTSGALVDVSEKSYSLTASIITRV 194

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFGKSFRGGELDNEKFEEVIRR LVV+GSFSATDFFPSFGWIIDWM+GVRGRLEKSF EL
Sbjct: 195 AFGKSFRGGELDNEKFEEVIRRALVVMGSFSATDFFPSFGWIIDWMSGVRGRLEKSFXEL 254

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIF+AGV
Sbjct: 255 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFIAGV 314

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DTGAGTIVWAM EL +NPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL
Sbjct: 315 DTGAGTIVWAMTELARNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 374

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID
Sbjct: 375 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 434

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL
Sbjct: 435 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 494

Query: 421 TVTKKSPLHLIPVEYFK 438
           TVTKKSPLHLIPVEYFK
Sbjct: 495 TVTKKSPLHLIPVEYFK 511

BLAST of Moc09g41010 vs. ExPASy TrEMBL
Match: A0A6J1DDC0 (cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019732 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 9.6e-245
Identity = 418/437 (95.65%), Postives = 428/437 (97.94%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           MRLHLGSVPTI ISSAAAARD+FKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE
Sbjct: 75  MRLHLGSVPTIXISSAAAARDMFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 134

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSST+GA VDVSEKSY LTASIITRV
Sbjct: 135 VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTSGALVDVSEKSYSLTASIITRV 194

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFGKSFRGGELDNEKFEEVIRR LVV+GSFSATDFFPSFGWIIDWM+GVRGRLEKSF EL
Sbjct: 195 AFGKSFRGGELDNEKFEEVIRRALVVMGSFSATDFFPSFGWIIDWMSGVRGRLEKSFLEL 254

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQL+RDCIKALTMDIF+AGV
Sbjct: 255 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLTRDCIKALTMDIFIAGV 314

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DTGAGTIVWAM EL +NPRVMKKLQDEIRSSIKEED +VKESHLEKLEYLKMVVKEVLRL
Sbjct: 315 DTGAGTIVWAMTELARNPRVMKKLQDEIRSSIKEEDRVVKESHLEKLEYLKMVVKEVLRL 374

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRETMSHFKLGGYDIEPKAHLHVN+WAIGRDPESWTDPDEFLPERFVGSNID
Sbjct: 375 HPPAPLLLPRETMSHFKLGGYDIEPKAHLHVNIWAIGRDPESWTDPDEFLPERFVGSNID 434

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLD+EEDAGL
Sbjct: 435 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDMEEDAGL 494

Query: 421 TVTKKSPLHLIPVEYFK 438
           TVTKKSPLHLIPVEYFK
Sbjct: 495 TVTKKSPLHLIPVEYFK 511

BLAST of Moc09g41010 vs. ExPASy TrEMBL
Match: A0A6J1GYQ4 (cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111458729 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.2e-188
Identity = 316/432 (73.15%), Postives = 375/432 (86.81%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPTIVISSAAAAR+LFK HDLASCSRP L+GSGRLSYN+LDISFSPYGDHWR 
Sbjct: 72  MLLQLGSVPTIVISSAAAARELFKFHDLASCSRPRLAGSGRLSYNYLDISFSPYGDHWRN 131

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKIC+LELF+ RRV+SFR +RE EVG L+NSI+QSS++ AP+D+SEKSY LTA+IITRV
Sbjct: 132 VRKICMLELFTSRRVESFREIREEEVGRLLNSISQSSSSSAPIDLSEKSYSLTANIITRV 191

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG  F GG+LD+E F+ V+   L  +GSFS TDF P+FGWIID +NGV GRLEKSF+E+
Sbjct: 192 AFGSIFSGGKLDDEHFQHVMHSALAAIGSFSMTDFIPTFGWIIDRLNGVHGRLEKSFAEM 251

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+FFQHV++ R+ F  +   + NI+DVLL+MERE SE DAL++++DC+KAL MDIFLAGV
Sbjct: 252 DAFFQHVVEDRINFKKSSKKEENIVDVLLRMERESSESDALKVTKDCVKALIMDIFLAGV 311

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           +TGAGTIVWAM EL++NPRVMKKLQ EIRS IKE  G VKE +LEKLEYLKMVVKEVLRL
Sbjct: 312 ETGAGTIVWAMTELVRNPRVMKKLQHEIRSCIKE--GSVKEINLEKLEYLKMVVKEVLRL 371

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRET+  FKL GYDI+PK+HLH+NVWAIGRDP+SWTDP+EF PERF+ SN+D
Sbjct: 372 HPPVPLLLPRETICPFKLNGYDIDPKSHLHINVWAIGRDPQSWTDPEEFFPERFIESNVD 431

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           ++GQNFEL+PFG GRR CPG++M T+ VELALAN+ LCFDWKLP GMKEED+D+EE+ GL
Sbjct: 432 YKGQNFELIPFGGGRRICPGINMGTLTVELALANLFLCFDWKLPNGMKEEDIDMEEEVGL 491

Query: 421 TVTKKSPLHLIP 433
           T+TKKSPL LIP
Sbjct: 492 TITKKSPLKLIP 501

BLAST of Moc09g41010 vs. ExPASy TrEMBL
Match: A0A6J1GWE9 (cytochrome P450 71B26-like OS=Cucurbita moschata OX=3662 GN=LOC111458144 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.1e-187
Identity = 316/432 (73.15%), Postives = 374/432 (86.57%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPTIVISSAAAAR+LFK HDLASCSRPPL  +GRLSYN+LD+S +PYG+HWR 
Sbjct: 78  MLLQLGSVPTIVISSAAAARELFKFHDLASCSRPPLHANGRLSYNYLDMSLTPYGEHWRN 137

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKIC+LELFS +RVQSF+ +RE EVG L+ SI+QSS++ +P+DVS+KSY LTA+IITRV
Sbjct: 138 VRKICMLELFSAQRVQSFQEIREEEVGRLVKSISQSSSSSSPIDVSDKSYSLTANIITRV 197

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG  F GG+LD+E F+ V+RR +  +GSFS TDFFP+FGWIID +NGV GRLEKSF  L
Sbjct: 198 AFGSIFSGGKLDDEHFQHVVRRAVAAIGSFSMTDFFPTFGWIIDRLNGVHGRLEKSFGVL 257

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+FFQ V+D+R+ F     ++ NI+DVLL+MERE SE DAL++++DCIKAL  DIFLAGV
Sbjct: 258 DAFFQSVVDNRINFKEISKNEENIVDVLLRMERESSESDALKVTQDCIKALIKDIFLAGV 317

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           +TGAGTIVWAMAEL++NPRVMKKLQDEIRS IKE+   VKE  LEKLEYLKMVVKEV+RL
Sbjct: 318 ETGAGTIVWAMAELVRNPRVMKKLQDEIRSGIKEDS--VKEIDLEKLEYLKMVVKEVMRL 377

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRET+S FKL GYDIEPKAHLH+NVWAIGRDP+ WT+P+EF PERF+GSNID
Sbjct: 378 HPPAPLLLPRETISPFKLNGYDIEPKAHLHINVWAIGRDPQYWTNPEEFFPERFIGSNID 437

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           ++GQNFELLPFG GRR CPG++MAT+ VELALAN+LLCFDWKLP  MKEED+D+EED GL
Sbjct: 438 YKGQNFELLPFGGGRRICPGINMATLTVELALANLLLCFDWKLPDEMKEEDVDMEEDVGL 497

Query: 421 TVTKKSPLHLIP 433
           TV +KSPL LIP
Sbjct: 498 TVAQKSPLQLIP 507

BLAST of Moc09g41010 vs. ExPASy TrEMBL
Match: A0A6J1GXN6 (cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111458143 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.7e-185
Identity = 310/433 (71.59%), Postives = 375/433 (86.61%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPT+VISSAAAAR+LFK HDLASCSRPPL  +GRLSYN+LD+S +PYG+HWR 
Sbjct: 78  MLLQLGSVPTVVISSAAAARELFKFHDLASCSRPPLHANGRLSYNYLDMSLAPYGEHWRN 137

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRKIC+LELFS +RVQSF+ +RE EVG L+NSI+QSS++ AP+D+S+KSY LTA+IITRV
Sbjct: 138 VRKICMLELFSAQRVQSFQEIREEEVGRLLNSISQSSSSSAPIDLSDKSYSLTANIITRV 197

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG  F GG+LD+E F+ V+   L  +GSFS TDF P+FGWIID +NGV GRLEKSF+E+
Sbjct: 198 AFGSIFSGGKLDDEHFQHVMHSALAAIGSFSMTDFIPTFGWIIDRLNGVHGRLEKSFAEM 257

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+FFQHV++ R+ F  +   + NI+DVLL+ME+E SE DAL++++DC+KAL MDIFLAGV
Sbjct: 258 DAFFQHVVEDRINFKKSSKKEENIVDVLLRMEKESSESDALKVTKDCVKALIMDIFLAGV 317

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           ++GAGTIVWAM+ELI+NPRVMKKLQ+EIRS IK++   VKE+ LEKLEYLKMVVKEVLRL
Sbjct: 318 ESGAGTIVWAMSELIRNPRVMKKLQNEIRSCIKKDS--VKETDLEKLEYLKMVVKEVLRL 377

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPRET+S FKL GYDI+PK HLH+NVWAIGRDP+SWTDP+EF PERF+GSNID
Sbjct: 378 HPPAPLLLPRETISPFKLNGYDIDPKTHLHINVWAIGRDPQSWTDPEEFFPERFIGSNID 437

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
           ++GQ+FE LPFG GRR CPGM+M T+ +ELALAN+L CFDWKLP GMKEED+D+EED GL
Sbjct: 438 YKGQHFEFLPFGGGRRICPGMNMGTLMMELALANLLFCFDWKLPDGMKEEDVDMEEDGGL 497

Query: 421 TVTKKSPLHLIPV 434
           TVTKKS L LIPV
Sbjct: 498 TVTKKSLLKLIPV 508

BLAST of Moc09g41010 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 466.5 bits (1199), Expect = 2.4e-131
Identity = 218/435 (50.11%), Postives = 316/435 (72.64%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L  GS+PT+V+SS+  A+ + K+HDL  CSRP L+G   LSYN+LDI+FSP+ D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           +R+ICV ELFSV+RVQSF+ ++E EV  LI+S+++S++ G PV++SEK   LT  +  + 
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
            FG +F+G  L++++FE++I    + +GSFSA+D+FP+ GWIIDW+ G+ G+ E+S   L
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+F++ + D  L      +   + +D+LL++E+E +     +L+R+ IKA+ M++ + G+
Sbjct: 246 DAFYEQMFD--LHKQGNKEGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGI 305

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
            T A T+ WAM EL++NPRVMKK+Q EIR+ I ++  I  +  +++L YLKMV+ E  RL
Sbjct: 306 GTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDD-IDQLHYLKMVINETWRL 365

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP P L+PR+ MS F+L  Y I  K  L+VNVWAIGRDP++W DP+EFLPERFV S+ID
Sbjct: 366 HPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSID 425

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
            +GQ+FELLPFG+GRR CP M+M T  VE  LANML  FDWK+P GM  ED+D+EE  GL
Sbjct: 426 AKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPGL 485

Query: 421 TVTKKSPLHLIPVEY 436
             +KK+ L L+P++Y
Sbjct: 486 NASKKNELVLVPLKY 497

BLAST of Moc09g41010 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 465.7 bits (1197), Expect = 4.1e-131
Identity = 224/436 (51.38%), Postives = 313/436 (71.79%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LG VPT+++SS+  A+   K+HDL  CSRP  +G+  LSYN+LDI+FSPY D+W+E
Sbjct: 66  MLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRK+ V ELFS ++V S + +++ EV  LI+SI++S+    P+++++    LT S++ R 
Sbjct: 126 VRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRT 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AF  +F G  L++E+F  ++R  L ++GSFSA+DF P  G IID + G++GR E+S  +L
Sbjct: 186 AFSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDL 245

Query: 181 DSFFQHVID-HRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAG 240
           D+F++ + D H+ K     +D    +D+LL++E+E +     +L+R+ IKA+ MD+ LAG
Sbjct: 246 DAFYEQMFDLHKQKKEEGSED---FVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAG 305

Query: 241 VDTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLR 300
           +DT A T+ WAMAEL KNPRVMKK+Q EIRS IK ++ I  +   +KLEYLKMV+KE  R
Sbjct: 306 MDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDD-TDKLEYLKMVIKETWR 365

Query: 301 LHPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNI 360
           LHP  PLL+PRE MS F++ GY I  K  LHVNVWAIGRDP++W DP+ FLPERF  +NI
Sbjct: 366 LHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNI 425

Query: 361 DFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAG 420
           D +GQ+FELLPFG GRR CP ++M T  VE  LAN+L  FDWKLP+GMK +D+D+EE  G
Sbjct: 426 DAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPG 485

Query: 421 LTVTKKSPLHLIPVEY 436
           LTV KK+ L L+P ++
Sbjct: 486 LTVNKKNELILVPTKF 497

BLAST of Moc09g41010 vs. TAIR 10
Match: AT2G02580.1 (cytochrome P450, family 71, subfamily B, polypeptide 9 )

HSP 1 Score: 465.3 bits (1196), Expect = 5.4e-131
Identity = 225/436 (51.61%), Postives = 314/436 (72.02%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LGSVPT+V+SS+  A+ + K++DL  CSRP L+G+  LSYN+LDI+FSP+ D+W+E
Sbjct: 66  MLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           +R+ICV ELFS +RV S + ++E EV  LI S  +S++  +PV++SEK   LT S+I + 
Sbjct: 126 LRRICVQELFSAKRVHSIQPIKEEEVRKLIVSATESASQKSPVNLSEKFLDLTVSVICKA 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AF   F    L+N+ F+++I    + +GSFSA++FFP+ GWIIDW+ G++ R EKS  +L
Sbjct: 186 AFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDL 245

Query: 181 DSFFQHVID-HRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAG 240
           D F+Q + D H+ +     +D    +D+LLK+E+E +     +L+R+ +KA+ M++ L  
Sbjct: 246 DVFYQQMFDLHKQENKQGVED---FVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLGA 305

Query: 241 VDTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLR 300
           ++T A T+ WAMAELI+NPRVMKK+Q EIR+ +  +  ++    ++ L YLKMV+KE  R
Sbjct: 306 INTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINK-SVITLDDIDHLPYLKMVIKETWR 365

Query: 301 LHPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNI 360
           LHPP PLLLPRE MS F++ GY I+PK  L+VNVWAIGRDP+SW D D F PERF+ +NI
Sbjct: 366 LHPPVPLLLPREVMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNI 425

Query: 361 DFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAG 420
           D +GQNFELLPFG+GRR CPGM+M T  VE  LANML  FDW++P GM  ED+D+EE  G
Sbjct: 426 DAKGQNFELLPFGSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPG 485

Query: 421 LTVTKKSPLHLIPVEY 436
           L V KK+ L L+PV+Y
Sbjct: 486 LAVGKKNELLLVPVKY 497

BLAST of Moc09g41010 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 464.5 bits (1194), Expect = 9.2e-131
Identity = 223/432 (51.62%), Postives = 312/432 (72.22%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LG VPT+V+SS+  AR + ++HDL  C+RP LSG   LSYN+LDI+FSPY D+W+E
Sbjct: 65  MHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKE 124

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           VRK+CV ELFS ++V S + +++ EV  +I+SIA+S++   PV+++ K   LT S++ R 
Sbjct: 125 VRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRT 184

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG SF G  L++++F +++R  L ++GSFSA DF P  GWIID + G++GR E+S  +L
Sbjct: 185 AFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDL 244

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           ++FF+ + D  L      + + + +D+LL++E+E +     +L+R+ IKA+ +D+ LAG+
Sbjct: 245 NAFFEQMFD--LHKEGKKEGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGI 304

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DT A T+ WAM EL +NPRVMKK+Q EIR+ +     I  E  +++LEYLKMV+KE  RL
Sbjct: 305 DTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFED-MDQLEYLKMVIKETWRL 364

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HP  PLLLPRE MS F + GY I  K  LHVNVWAIGRDP++W DP+ FLPERF+ +NID
Sbjct: 365 HPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDNNID 424

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
            +GQ+FELLPFG GRR CP ++M T  VE  LAN+L  FDWKLP+G++ +D+DVEE  GL
Sbjct: 425 AKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEAPGL 484

Query: 421 TVTKKSPLHLIP 433
           TV KK+ L L+P
Sbjct: 485 TVNKKNELLLVP 493

BLAST of Moc09g41010 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 463.8 bits (1192), Expect = 1.6e-130
Identity = 226/435 (51.95%), Postives = 313/435 (71.95%), Query Frame = 0

Query: 1   MRLHLGSVPTIVISSAAAARDLFKLHDLASCSRPPLSGSGRLSYNFLDISFSPYGDHWRE 60
           M L LG VPT+++SS+  A+   + +DL  CSRP L+G   LSYN LD+S SPY ++W+E
Sbjct: 66  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 125

Query: 61  VRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTAGAPVDVSEKSYCLTASIITRV 120
           +RK+C  ELFS  ++QS + +++ EV  +I+SIA+SS+   PV++S+    LT S++ + 
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 121 AFGKSFRGGELDNEKFEEVIRRGLVVVGSFSATDFFPSFGWIIDWMNGVRGRLEKSFSEL 180
           AFG SF G  L++++F +++R    ++GSFSA+DF P  GWIID  NG++G  +KSF +L
Sbjct: 186 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 245

Query: 181 DSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLSRDCIKALTMDIFLAGV 240
           D+F++ + D   +      +D  ++DVLL++E+E       +L+R+ IKA+ M+I L G+
Sbjct: 246 DAFYEQIFDLHKEEKEVGSED--LVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLGGI 305

Query: 241 DTGAGTIVWAMAELIKNPRVMKKLQDEIRSSIKEEDGIVKESHLEKLEYLKMVVKEVLRL 300
           DT A T+ WAMAEL KNPRVMKK+Q EIR+ IK ++ I  +   +KLEYLKMV+KE  RL
Sbjct: 306 DTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDD-TDKLEYLKMVIKETWRL 365

Query: 301 HPPGPLLLPRETMSHFKLGGYDIEPKAHLHVNVWAIGRDPESWTDPDEFLPERFVGSNID 360
           HPP PLLLPR+ ++ F++ GY I  K  LHVNVWAIGRDP++W DP+ FLPERF  SNID
Sbjct: 366 HPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNID 425

Query: 361 FRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQGMKEEDLDVEEDAGL 420
            +GQNFELL FG+GRR CPG++M T  VE  LANML  FDWKLP+GM  ED+D+EE  GL
Sbjct: 426 AKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPGL 485

Query: 421 TVTKKSPLHLIPVEY 436
           TV+KKS L L+PV+Y
Sbjct: 486 TVSKKSELVLVPVKY 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151852.14.3e-24797.03cytochrome P450 71B34-like isoform X1 [Momordica charantia][more]
XP_022151853.12.0e-24495.65cytochrome P450 71B34-like isoform X2 [Momordica charantia][more]
XP_022957287.14.5e-18873.15cytochrome P450 71B34-like [Cucurbita moschata][more]
XP_022956391.16.5e-18773.15cytochrome P450 71B26-like [Cucurbita moschata][more]
XP_023529060.12.7e-18572.75cytochrome P450 71B26-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LIP43.4e-13050.11Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1[more]
Q9LIP65.8e-13051.38Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1[more]
O647187.6e-13051.61Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3[more]
Q9LIP51.3e-12951.62Cytochrome P450 71B35 OS=Arabidopsis thaliana OX=3702 GN=CYP71B35 PE=2 SV=1[more]
Q9LTL02.2e-12951.95Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DEL12.1e-24797.03cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1DDC09.6e-24595.65cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1GYQ42.2e-18873.15cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111458729 PE=3 SV... [more]
A0A6J1GWE93.1e-18773.15cytochrome P450 71B26-like OS=Cucurbita moschata OX=3662 GN=LOC111458144 PE=3 SV... [more]
A0A6J1GXN61.7e-18571.59cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111458143 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT3G26320.12.4e-13150.11cytochrome P450, family 71, subfamily B, polypeptide 36 [more]
AT3G26300.14.1e-13151.38cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT2G02580.15.4e-13151.61cytochrome P450, family 71, subfamily B, polypeptide 9 [more]
AT3G26310.19.2e-13151.62cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G26290.11.6e-13051.95cytochrome P450, family 71, subfamily B, polypeptide 26 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 292..303
score: 43.62
coord: 238..255
score: 34.93
coord: 369..378
score: 62.69
coord: 378..389
score: 28.56
IPR001128Cytochrome P450PFAMPF00067p450coord: 1..425
e-value: 1.7E-82
score: 277.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 368..378
score: 59.17
coord: 378..401
score: 29.56
coord: 291..309
score: 36.56
coord: 332..356
score: 37.59
coord: 112..130
score: 25.1
coord: 247..273
score: 32.92
coord: 227..244
score: 32.02
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..433
e-value: 6.5E-108
score: 363.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 1..432
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 1..434
NoneNo IPR availablePANTHERPTHR47956:SF5CYTOCHROME P450 71B16-RELATEDcoord: 1..434
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 371..380

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g41010.1Moc09g41010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen