Moc09g40250 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g40250
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr9: 30782722 .. 30787862 (+)
RNA-Seq Expression	Moc09g40250
Synteny	Moc09g40250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAACCCGGTTATGGTTCAACCAATAACTGGAAGTCCCTCTCGGCCAATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCGGAGTCAGCATTTAAGGGAACACTCATCTACTCCCCTAACGTCGGGAAGGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGCTCCCCACTCGGTCTCGTCCCCAAAATGGTAGGAATGTTGAGTCGGCAACTCGGGCCACTCTCACCCATACAGATCAAAGAACGAGCCCTCACGGGCAAAAGTCCATAACTCACTCAGGATTGAGAATGAGTTGCCTAGTCATCCTAAGAAATGACAATCTATTAGTTAACGGTGTTACACCTAAAGATTGCCTATTTCGCGGTCCGGTCTTATACAAACTCATTATATAGAATATCCCCACTCGCATGTCTCCACACGAACGACCTGGATCGAGTCGTCTGTAACCTTTACAGAGTGGGCCGTATCCATAGTGTTACCAGGATAAGGTACCCAGCCTTATCCATATACTATAGACCCTTTAGGTTATTCACGGGACATTATCCATTCATATATTAGGGGTATATCAAATATATACCCCGTATATCAACTATATACCCAGTATATATATAGTCCCGATTACATCATAAATAACCTTGGATGTTTAGTTTATTGGATTTTGGAAAATGCAAAGCTATGATTCAACAACAAATTTATTGAATAAAACTTTATTAAATAGATATATGCTTTTACAAAATACAAACTACGAGTTTAGGGCAACACACCCAACAAACTCCCACTTGAACTAAACTCGAGTGGGGTGTACATTATCATAAAGCAAAGGGAAAATACAATAAACTAGGACATACACTATACCCAATTACATTTCTCCCACTTGCCCTAGACTAACTGTACAGAGTTCGTAAACCTAGACTTTCTAGATGGCTCTCAAACACTTTAGCCGTGAGTGCCTTTGTAAAAGGATCAGCAATATTGTGCTCTGTAGCGATCTTCGTGACAATCACATCTCCTCGCTGCACTATCTCTCGGCTTCGAGGTTCTTTAGAATTTGTCACAGCACCACTGTTATCACAATAAAGTGTGATGGGCAAGTTCATGTTTGGAACGACGTCCAAATCTGTCAAGAACTTTCTAAGCCAAACCGCCTCTTTTGCTGCTTCACAAGCTGCAACATACTCAGCCTCCATCGTGGAGTCTGCGATGCATCCTTGTTTGATGCATCGTGGATTTCCTTGAATCCTTGTCTTTTTGGAAGTCAGAATTAGTGTATCCTGTAAGGATCAAATCCTTAGCGCCATACACGAGCTTGTAGTCCCTCGTTCTCCTAAGATACTTGAGGATTCCCTTAACAGCAGTCCAGTGATCATATCCTGGGTTGGATTGGTACCTACTGACTATCCCTACCGCATAGCAAATGTCAGGCCTAGTGCACAGCATAGCGTACATCAGGCTGCCCACTGCTGAAACATAGGGTATACGTCTCATATCCTCAACTTCTTGAGGTGTCTTAGGACACTGCTCCTTAGACAGATGAATTCCATGTCTGAAGGGTAATAAACCTTTCTTGGAATTCTGCATCGAATATCGAGCAAGCATCTTGTCGATATAAGTTGCTTGAGACAGGGCTAACGTTTTGTTCTTGCGATCCCTTAGAATTTGGATGCCAAGAACATACTGAGCTTCTCCCAAATCCTTCATTTGGAACTCAGTCGCTAGCCACTTCTTTATGTCAGTAAGGTATCCTATATCATTCCCAATGAGTAGAATATCATCCACATACAACACTAAGAAGGCTACAGTGTTGTTGACAATCTTCTTGTATACACAAGGCTCATCAACGTTTTGGTCAAAACCATAAGATTTGATCGTTGTATCGAACCTTATGTTCCAAGATCGAGATGCTTGTTTTAGTCCATATATGGACCATTTAAGCTTGCAAACCTTTTGCTCTTGACCTTGTTCTATGAACCCTTTGGAGGTTGAATCCACTCCACAACATTTAATTTTCTTTTGGATACACGGATAAAGTCCCTCCATAATTTCAAGTACGAGCATCTATATCTTAACGCGTTTTTTATGAAAGAAACATGTACGAGTGAAATATTTATTTATGACTTTGTTTTTTATGATTTTTTTTTCCAAAATTATCTATAAAGATGTTATTTTTCATCAAATCAATAAAACTTTTGAGTTTATTTAGTACAAAAATTCAGAATCTCTTGGTTGAAGATATATATCAATTATTGTTGAATTATGTTCGTTTTCGCCATTGACATTCAAATGCTTGGTAAATATAACATTTAGAATATCTAATTGGTGGTTGTGCATTGTGTAATCATCTCCCCCACCTATTAACAAAGACTTTTAAATTCGTATTTAGAATTTTCTTTTCTTGTTTTATAACATGTAGAGTGGAAATTATTTAAAACTTCTCTTGTGTTTTGAAATATATGTTTTAATTAGTTGAGTTATGTTTAAGTTGGCATAGAAAATATTATTAGAGAAAAAGTAACCTAAAAAATAGTGATTTTTTTAGCTTGTAACATAATTAAGTTTTCACATGTTTAAATATGTATATATATTTTAATATAAAAAAAAAGTATGTATACATGTGTGAATAAATATAAGTAATATATTTTTTATTTCTTACAAGGTATTAGAAATTTAAAAAATAAAAATGTTATTATTTTTTTCTCTAGAAGTCAGATACGTGACTAAGATTATTTAAGTCTGTTCCTTGAAATTAGGGAATGAGGCAATAAAAGAGATAAAAAGTCTTCTCAGCCCTAACCTCCTGCCACGATTTCATCTCTACATAATAAATAAAAAATTTAGGTATTATAGTATAATTTTTTTCTTTTGACATTTAATATAATATAATTGAACAGTATAAAATTTAAAGTCTATATATTGTAGTTACGAAAAGTATTTGGAAAAGTATTGGGCGTTTTAGTCAATTTGATTTGAAGATGCTTTTAAACTTCAATTCTATTATTAATTAATCAATGAAGTATGCATTGACTAATAAAAATTGATCCCATTAGTAAAATATTATTGAATAAATTTAACTATTAATTATTCCTTCACCTCAATATATTTACATATAAAAATGAACTTTTCATGTCTCTCACTCTAGTTTAGTCCAAGCTAAAGTTAGAAGTATATATCTCCAACTAAAAAGTTTGAAGTTTGAATTTCTATTCTCACATGTTCTTGAATTAAAAAATTTTTTTAGCTTAATCTATTTTAATCATATCTTAAAAAAAGTATATTTTAATTTAAACATTAATTTTGCGTATGTACTTACATGTGAGTATTAATTTTTTTATTAGTACAACAAGTACGATGAAGATTCAATCTCATATTGTTTTGGTTAGACATAAGTGCTTTGACCAGTCGAAACTGAAAATGAAAAATGATCTTACAAAAGTTTATAATAGATATAGGTTGATACATCTCATTGTCAATTGATTTTGAGATGAAACTTGTACGTATGGTTAATCACTAATATTAATAAATAGTATTAAATTATATATTCAATAAATTTTAACATTCAATTTCTAGTTCAAATTTTAATATTCATTAATATAGTTGAAACATCCTTTTGAGAACCAAATCTTCCACCCTAACCTTAAACTTTAACATTTTAAGTAAGTATTATTTATTGAGTAACATTTTAAGTAAGTATTATTTATTGAGTTAGGTTCAAATTAAGTAATATAGTTGGAACCCTTTTGAATTAATATAAGATATAAAGATCTTAAAATTTTCAAAGGGACTAAAATCACAGAAGTATAATATAAAATGTAGAATTTAACACTGTTTACAATTATTTATAAAACAAACTAAAAGTGACAATAAAAAAAAGTAATATTTTCGATTTGTATGATAAGTCAAAAATAAACAACAAAAGCTATGTAAAGTATTAAGCACGACAATACTATGATAAATATGATGGAATTAAAAATGTGAACGTGGATAAAAATTTCAAACTTTTCAAAAATATTGTAAAAAGTAACAATAGAATGTAAATATGGATGTGAATATTTTCAAAAAAGAAAAAGTAAAAAAAAAAAAAAAAAAAACCAATTTTGACAAGTAAAATATATAAGTAATCATTGTTAGCTTTAGGTAGCACACTAAACTTATCAATAAATGCCTAAACTCAATCATTACAATAATCGACCTAATCACAAAGCAAGGTAGATTAAAAAAAAAACAAAAAAGCACTAAAATCGAAGAGAAAAGAAATATATTTAAATCATTTAATGGAATTGGTAAAGTTAAAAGTCTATGAAAATAGTTTTAAAAAAAATTTTAAAAATAGTCTCATCCACTCAAATAAAGACTTAACTTTAACCTATAGCTTTTTCCTTCTAAAATACTAATCTCCAATGAATGGAAATCTCAGTTTTATGGACATTTGGCTACATCTTTATGATAAACTAAATCCACAGCAGGAGATCTTGCTGCTTTTACCTGTTGGGCGATTTGGTGTGATCGAAATTCTCTAGCCCATGGTTCTTCTGTCTCCACCCCTGCTTTAAGGTACAATTGGATTAGTTCTTATTTTCAGAATTACTCACAAGCTCAAGAAAATAAGAAAATCTCACCGCAGCAGAATTCGGTCCCTCCCCCATCCTGTCGTTGGCTTCCTCCTTGCGACTCAGCTATGAAGGTGAACTCTGATGCAGCCTGTCGTTCTACATCAACAGGGCTAGGTCTTATTATCAGAGACCATTTGGGGGTTTTGTTGGTAGCGAAATCAGTGTTTCTGCCGATGCCATTGAACCCTCTTTTTGCCGAAATCAGAGGAATTTTGGAAGCTCTAAAGCTCGCTGCCTCACGGAGTTACACTCGTCTGGTTGTAGAATCTGATTGCCAAGAAGCCATTCGCCTTGTTCAAGGAGATCTGTTCACGATGGGAAAAGAAATGAATTGGATTATGAAAATTCGATCGATAGCCCTAGCGTTCGAATCAATTTCCTTCATGCACTCCCCCCGTTCGTGTAATTTTTGTGCGCATTCCTTAGCCAAATGGGCAATTTCGGCCTCATCCTCGTGTTTATGGCAGTTTCCCTTCTCCTCTCGATTAATGGATCTTGTAAAAGGGGATTCCCTTCAACCGTTGCCCTAG

mRNA sequence

ATGAACAACCCGGTTATGGTTCAACCAATAACTGGAAGTCCCTCTCGGCCAATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCGGAGTCAGCATTTAAGGGAACACTCATCTACTCCCCTAACGTCGGGAAGGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGCTCCCCACTCGGTCTCGTCCCCAAAATGGTAGGAATGTTGAGTCGGCAACTCGGGCCACTCTCACCCATACAGATCAAAGAACGAGCCCTCACGGGCAAAAGTCCATAACTCACTCAGGATTGAGAATGAGTTGCCTAGTCATCCTAAGAAATGACAATCTATTAGTTAACGGTGTTACACCTAAAGATTGCCTATTTCGCGGAGATCTTGCTGCTTTTACCTGTTGGGCGATTTGGTGTGATCGAAATTCTCTAGCCCATGGTTCTTCTGTCTCCACCCCTGCTTTAAGGTACAATTGGATTAGTTCTTATTTTCAGAATTACTCACAAGCTCAAGAAAATAAGAAAATCTCACCGCAGCAGAATTCGGTCCCTCCCCCATCCTGTCGTTGGCTTCCTCCTTGCGACTCAGCTATGAAGGTGAACTCTGATGCAGCCTGTCGTTCTACATCAACAGGGCTAGGTCTTATTATCAGAGACCATTTGGGGGTTTTGTTGGTAGCGAAATCAGTGTTTCTGCCGATGCCATTGAACCCTCTTTTTGCCGAAATCAGAGGAATTTTGGAAGCTCTAAAGCTCGCTGCCTCACGGAGTTACACTCGTCTGGTTGTAGAATCTGATTGCCAAGAAGCCATTCGCCTTGTTCAAGGAGATCTGTTCACGATGGGAAAAGAAATGAATTGGATTATGAAAATTCGATCGATAGCCCTAGCGTTCGAATCAATTTCCTTCATGCACTCCCCCCGTTCGTGTAATTTTTGTGCGCATTCCTTAGCCAAATGGGCAATTTCGGCCTCATCCTCGTGTTTATGGCAGTTTCCCTTCTCCTCTCGATTAATGGATCTTGTAAAAGGGGATTCCCTTCAACCGTTGCCCTAG

Coding sequence (CDS)

ATGAACAACCCGGTTATGGTTCAACCAATAACTGGAAGTCCCTCTCGGCCAATGAGAGGGTGGGGCCCCTTGTTCAAGTCCCGGAGTCAGCATTTAAGGGAACACTCATCTACTCCCCTAACGTCGGGAAGGAGTGAATTCCATCTTGTGAAGTTATGTTCCCAGCTCCCCACTCGGTCTCGTCCCCAAAATGGTAGGAATGTTGAGTCGGCAACTCGGGCCACTCTCACCCATACAGATCAAAGAACGAGCCCTCACGGGCAAAAGTCCATAACTCACTCAGGATTGAGAATGAGTTGCCTAGTCATCCTAAGAAATGACAATCTATTAGTTAACGGTGTTACACCTAAAGATTGCCTATTTCGCGGAGATCTTGCTGCTTTTACCTGTTGGGCGATTTGGTGTGATCGAAATTCTCTAGCCCATGGTTCTTCTGTCTCCACCCCTGCTTTAAGGTACAATTGGATTAGTTCTTATTTTCAGAATTACTCACAAGCTCAAGAAAATAAGAAAATCTCACCGCAGCAGAATTCGGTCCCTCCCCCATCCTGTCGTTGGCTTCCTCCTTGCGACTCAGCTATGAAGGTGAACTCTGATGCAGCCTGTCGTTCTACATCAACAGGGCTAGGTCTTATTATCAGAGACCATTTGGGGGTTTTGTTGGTAGCGAAATCAGTGTTTCTGCCGATGCCATTGAACCCTCTTTTTGCCGAAATCAGAGGAATTTTGGAAGCTCTAAAGCTCGCTGCCTCACGGAGTTACACTCGTCTGGTTGTAGAATCTGATTGCCAAGAAGCCATTCGCCTTGTTCAAGGAGATCTGTTCACGATGGGAAAAGAAATGAATTGGATTATGAAAATTCGATCGATAGCCCTAGCGTTCGAATCAATTTCCTTCATGCACTCCCCCCGTTCGTGTAATTTTTGTGCGCATTCCTTAGCCAAATGGGCAATTTCGGCCTCATCCTCGTGTTTATGGCAGTTTCCCTTCTCCTCTCGATTAATGGATCTTGTAAAAGGGGATTCCCTTCAACCGTTGCCCTAG

Protein sequence

MNNPVMVQPITGSPSRPMRGWGPLFKSRSQHLREHSSTPLTSGRSEFHLVKLCSQLPTRSRPQNGRNVESATRATLTHTDQRTSPHGQKSITHSGLRMSCLVILRNDNLLVNGVTPKDCLFRGDLAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKKISPQQNSVPPPSCRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRSIALAFESISFMHSPRSCNFCAHSLAKWAISASSSCLWQFPFSSRLMDLVKGDSLQPLP

Homology

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BLAST of Moc09g40250 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 298.1 bits (762), Expect = 9.7e-77
Identity = 148/163 (90.80%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 124 DLAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKKISPQQNSVPPPS 183
           DLAAFT WAIWCDRNS AHGSSVSTPALR NWISSYFQNYSQAQENK+ISPQQ+SVPPPS
Sbjct: 53  DLAAFTXWAIWCDRNSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPS 112

Query: 184 CRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGIL 243
           CRWLPP DSAMKVNSDAACRSTSTGLGLIIRDH GVLLVAKS+FLPMPLNPLFAEIRGIL
Sbjct: 113 CRWLPPRDSAMKVNSDAACRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGIL 172

Query: 244 EALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMK 287
           EALKLAASRSYTRLVVESDCQEAIRLVQGDLF+MG+E+  + K
Sbjct: 173 EALKLAASRSYTRLVVESDCQEAIRLVQGDLFSMGEELIGLWK 215

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BLAST of Moc09g40250 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 190.3 bits (482), Expect = 2.8e-44
Identity = 104/233 (44.64%), Postives = 141/233 (60.52%), Query Frame = 0


Query: 110  LVNGVTPKDCLFRGDLAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQEN 169
            L   + PKD     +LAA T W IW DRNSL HG  VS    +  W++ +  ++SQAQ +
Sbjct: 1222 LTEQLEPKDL----NLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMS 1281

Query: 170  KKISPQQNSVPPPSCRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLP 229
                  Q++  P    W P    ++K+N+DAACR  ST  G IIRD    L+ A S+ +P
Sbjct: 1282 NYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAACRGASTSFGCIIRDSSCSLVAATSIRVP 1341

Query: 230  MPLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRS 289
             PL+PL AEIR ILE LK AA+ ++T L VESD   AI+L++ ++ T G E NW+M+I++
Sbjct: 1342 FPLSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQA 1401

Query: 290  IALAFESISFMHSPRSCNFCAHSLAKWAI-SASSSCLWQFPFSSRLMDLVKGD 342
            +   F  ISF HS R CN  AH LAKW I S S++  W F F + L+DLV+ D
Sbjct: 1402 LTCCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNFPTWLLDLVQRD 1450

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BLAST of Moc09g40250 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-18
Identity = 65/208 (31.25%), Postives = 99/208 (47.60%), Query Frame = 0


Query: 125 LAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQEN--KKISPQQNSVPPP 184
           L   TCWA+W DR++L +   +    ++  WI  Y +       N  K+  P+ N     
Sbjct: 11  LTGITCWALWNDRDALINKKKIPEALIKVEWILKYAEEVRMKYHNGMKRRIPRVN----- 70

Query: 185 SCRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGI 244
              W PP    +K+N+DAA     +G+G+++R+     +V   V   M   PL A+I  I
Sbjct: 71  --EWRPPLGGVVKMNTDAAVSEEGSGVGVLLREG-NAEIVGAMVDFHMVKTPLLAKILAI 130

Query: 245 LEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRSIALAFESISFMHS 304
            E L LA      R+VVE+D  EA+ L+       G+ ++W+  IR+ A  F+ I F H 
Sbjct: 131 REGLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHV 190

Query: 305 PRSCNFCAHSLAKWAISASSSCLWQFPF 331
            R  N  A+ L +  IS     LW+  F
Sbjct: 191 FRESNAVAYHLGRETISLRCGFLWRLDF 210

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BLAST of Moc09g40250 vs. NCBI nr
Match: XP_038722072.1 (uncharacterized protein LOC120014227 [Tripterygium wilfordii])

HSP 1 Score: 104.8 bits (260), Expect = 1.6e-18
Identity = 68/217 (31.34%), Postives = 111/217 (51.15%), Query Frame = 0


Query: 128 FTC-WAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKKISPQQNSVPPPSCRW 187
           FTC W IW  RN + H +           ++++  +Y +AQ        + S PP   +W
Sbjct: 71  FTCLWHIWRMRNQILHNNRSFKSEEVGRMMTTFLADYQEAQPPNLHGSSRISQPP--IKW 130

Query: 188 LPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGILEAL 247
            PP  S +K+N DAA R +  G+G+I RD  G  + +K++ +P PLNPLFAE     EAL
Sbjct: 131 RPPQTSVIKINFDAALRDSKVGIGVIARDAEGTHIASKTICVPGPLNPLFAESIAAKEAL 190

Query: 248 KLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRSIALAFESISFMHSPRSC 307
             A S +Y  +V+E D    I+ ++ +   + +    +   +++  +   +SF H  RS 
Sbjct: 191 VFAKSLNYHDVVLEGDSLLVIKALERNEVDLSENGLCVASTKNLLDSSVHVSFSHVKRSA 250

Query: 308 NFCAHSLAKWAISASSSCLWQFPFSSRLMDLVKGDSL 344
           N+ A +LAK+A+S   S  W     + L  +++ D L
Sbjct: 251 NYVADTLAKYALSNYVSHEWLGEKPAFLRHVIETDCL 285

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BLAST of Moc09g40250 vs. NCBI nr
Match: XP_023897447.1 (uncharacterized protein LOC112009345 [Quercus suber])

HSP 1 Score: 102.8 bits (255), Expect = 6.0e-18
Identity = 62/204 (30.39%), Postives = 97/204 (47.55%), Query Frame = 0


Query: 125  LAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKKISPQQNSVPPPSC 184
            L     WA WC+RN + HG+   +P     W++ Y   YS A E+     ++ SV     
Sbjct: 924  LVVTVAWAFWCNRNEIRHGAEKKSPEAIVQWVNRYLLEYSAATESVPAVREEVSV----- 983

Query: 185  RWLPPCDSAMKVNSDAACRS--TSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGI 244
             W PP  S +KVN D A        G+G ++RD  G ++ A S  +P PL PL  E++  
Sbjct: 984  TWNPPPPSILKVNVDGATTKNLNFVGVGAVVRDEQGRVVAAMSRKIPAPLGPLEVEVKAF 1043

Query: 245  LEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRSIALAFESISFMHS 304
               L+LA    Y  +++E D    +R + G   +     + I+ ++     F ++   H 
Sbjct: 1044 EAGLQLAKDMGYQNIILEGDSLIIVRALCGISLSSSTIDSMIVGMQLFCSDFCTVYVSHV 1103

Query: 305  PRSCNFCAHSLAKWAISASSSCLW 327
             R  N  AH LAK+A+S + S +W
Sbjct: 1104 KRQENKPAHVLAKYALSINDSVVW 1122

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BLAST of Moc09g40250 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 4.7e-77
Identity = 148/163 (90.80%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 124 DLAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKKISPQQNSVPPPS 183
           DLAAFT WAIWCDRNS AHGSSVSTPALR NWISSYFQNYSQAQENK+ISPQQ+SVPPPS
Sbjct: 53  DLAAFTXWAIWCDRNSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPS 112

Query: 184 CRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGIL 243
           CRWLPP DSAMKVNSDAACRSTSTGLGLIIRDH GVLLVAKS+FLPMPLNPLFAEIRGIL
Sbjct: 113 CRWLPPRDSAMKVNSDAACRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGIL 172

Query: 244 EALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMK 287
           EALKLAASRSYTRLVVESDCQEAIRLVQGDLF+MG+E+  + K
Sbjct: 173 EALKLAASRSYTRLVVESDCQEAIRLVQGDLFSMGEELIGLWK 215

[Close]

BLAST of Moc09g40250 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.4e-44
Identity = 104/233 (44.64%), Postives = 141/233 (60.52%), Query Frame = 0


Query: 110  LVNGVTPKDCLFRGDLAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQEN 169
            L   + PKD     +LAA T W IW DRNSL HG  VS    +  W++ +  ++SQAQ +
Sbjct: 1222 LTEQLEPKDL----NLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQMS 1281

Query: 170  KKISPQQNSVPPPSCRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLP 229
                  Q++  P    W P    ++K+N+DAACR  ST  G IIRD    L+ A S+ +P
Sbjct: 1282 NYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAACRGASTSFGCIIRDSSCSLVAATSIRVP 1341

Query: 230  MPLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRS 289
             PL+PL AEIR ILE LK AA+ ++T L VESD   AI+L++ ++ T G E NW+M+I++
Sbjct: 1342 FPLSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQA 1401

Query: 290  IALAFESISFMHSPRSCNFCAHSLAKWAI-SASSSCLWQFPFSSRLMDLVKGD 342
            +   F  ISF HS R CN  AH LAKW I S S++  W F F + L+DLV+ D
Sbjct: 1402 LTCCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNFPTWLLDLVQRD 1450

[Close]

BLAST of Moc09g40250 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 5.8e-19
Identity = 65/208 (31.25%), Postives = 99/208 (47.60%), Query Frame = 0


Query: 125 LAAFTCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQEN--KKISPQQNSVPPP 184
           L   TCWA+W DR++L +   +    ++  WI  Y +       N  K+  P+ N     
Sbjct: 11  LTGITCWALWNDRDALINKKKIPEALIKVEWILKYAEEVRMKYHNGMKRRIPRVN----- 70

Query: 185 SCRWLPPCDSAMKVNSDAACRSTSTGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGI 244
              W PP    +K+N+DAA     +G+G+++R+     +V   V   M   PL A+I  I
Sbjct: 71  --EWRPPLGGVVKMNTDAAVSEEGSGVGVLLREG-NAEIVGAMVDFHMVKTPLLAKILAI 130

Query: 245 LEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRSIALAFESISFMHS 304
            E L LA      R+VVE+D  EA+ L+       G+ ++W+  IR+ A  F+ I F H 
Sbjct: 131 REGLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHV 190

Query: 305 PRSCNFCAHSLAKWAISASSSCLWQFPF 331
            R  N  A+ L +  IS     LW+  F
Sbjct: 191 FRESNAVAYHLGRETISLRCGFLWRLDF 210

[Close]

BLAST of Moc09g40250 vs. ExPASy TrEMBL
Match: A0A803Q1F8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.2e-16
Identity = 68/217 (31.34%), Postives = 105/217 (48.39%), Query Frame = 0


Query: 114  VTPKDCLFRGDLAAF--TCWAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKK 173
            +T KD L + +   F    W IW  RN     +      +   WI    +         +
Sbjct: 1517 MTMKDKLTKDEFLFFIGLTWLIWYRRNKCIFQNKDVADNI---WIPWAIEMLELHLATDR 1576

Query: 174  ISPQQNSVPPPSCRWLPPCDSAMKVNSDAAC--RSTSTGLGLIIRDHLGVLLVAKSVFLP 233
            ISP Q  +  P+  W PP   +  +NSD +        GLG+IIRDHLG L+ A++VF+P
Sbjct: 1577 ISPNQKQLKAPA-NWSPPLPGSFLINSDVSLIDGQPGCGLGVIIRDHLGALVAAETVFVP 1636

Query: 234  MPLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIMKIRS 293
              L+ L AE   I  ALK+A   S  ++ + SD Q  I+ + G+  +     + ++    
Sbjct: 1637 GCLSVLLAETMAIRLALKIAEKWSLQKVCISSDNQVVIQALTGNARSNTDWGHLVVDCLL 1696

Query: 294  IALAFESISFMHSPRSCNFCAHSLAKWAISASSSCLW 327
               +F+++SF+ SP++CN  AH LAKW      S +W
Sbjct: 1697 ARKSFQNLSFIFSPKNCNKVAHCLAKWGRLCQVSEVW 1729

[Close]

BLAST of Moc09g40250 vs. ExPASy TrEMBL
Match: A0A4Q3DBE2 (Reverse transcriptase-like protein (Fragment) OS=Rickettsiaceae bacterium OX=2023360 GN=EOP33_09975 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.6e-16
Identity = 58/199 (29.15%), Postives = 97/199 (48.74%), Query Frame = 0


Query: 131 WAIWCDRNSLAHGSSVSTPALRYNWISSYFQNYSQAQENKKISPQQNSVPPPSCRWLPPC 190
           W +W  RN++  G S  +      W  S+ + Y  A   +  +  + +  P   RW PP 
Sbjct: 78  WELWNHRNNVLAGRSAWSQPQLVQWTVSFLEEYRAATVGETPTSTRVTTTP---RWTPPR 137

Query: 191 DSAMKVNSDAACRST--STGLGLIIRDHLGVLLVAKSVFLPMPLNPLFAEIRGILEALKL 250
           D  + +N DAA        G+G I+RDH G ++ ++++FL    +P  AE   +L+ ++L
Sbjct: 138 DGTLSINVDAALARDRGGFGVGCIVRDHTGEVIASQTLFLARGFSPQLAEAYAVLQGIEL 197

Query: 251 AASRSYTRLVVESDCQEAIRLVQGDLFTMGKEMNWIM-KIRSIALAFESISFMHSPRSCN 310
             S    R  ++SDC   +R++   + T   E+  I+ +I       E    MH  R CN
Sbjct: 198 VRSVGGRRAEIQSDCLGVVRMIDEGV-TPATELGTILHRIFREVRELEVFRIMHISRLCN 257

Query: 311 FCAHSLAKWAISASSSCLW 327
             AH+LA++A++  SS  W
Sbjct: 258 VAAHNLARYAVNHHSSSTW 272

The following BLAST results are available for this feature:

BLAST of Moc09g40250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131661.1	9.7e-77	90.80	uncharacterized protein LOC111004786 [Momordica charantia]	[more]
XP_022158377.1	2.8e-44	44.64	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_022158489.1	1.2e-18	31.25	uncharacterized protein LOC111024968 [Momordica charantia]	[more]
XP_038722072.1	1.6e-18	31.34	uncharacterized protein LOC120014227 [Tripterygium wilfordii]	[more]
XP_023897447.1	6.0e-18	30.39	uncharacterized protein LOC112009345 [Quercus suber]	[more]

BLAST of Moc09g40250 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc09g40250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQ49	4.7e-77	90.80	uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004...	[more]
A0A6J1DX30	1.4e-44	44.64	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J1DZK3	5.8e-19	31.25	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A803Q1F8	1.2e-16	31.34	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A4Q3DBE2	4.6e-16	29.15	Reverse transcriptase-like protein (Fragment) OS=Rickettsiaceae bacterium OX=202...	[more]

BLAST of Moc09g40250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 197..317 e-value: 1.1E-22 score: 80.1
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 192..319 e-value: 6.3E-15 score: 57.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..41
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..91
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 185..321
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 185..321
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 196..315 e-value: 4.14722E-29 score: 107.015
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 194..320

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g40250.1	Moc09g40250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity