Moc09g37040 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g37040
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectinesterase
Locationchr9: 28523511 .. 28528151 (-)
RNA-Seq ExpressionMoc09g37040
SyntenyMoc09g37040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCACTTCCCTACATTTTTCTTCTCCTTCTCTCCCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCTTCCGCCGCGCTCTGTAAAACCACTCTCTACCCTAAGCTCTGCCATTCGATTCTCTCCTCCATCCGATTCTCGCCCTCCGATCCCTACGGCTACGGAAAATTCTCCGTCAAACAGTGTCTGAAGCAGGCGATCAAAATGTCGAGCGTAATCGGCGGCTACCTTCGCCGTGGAGGCGGTTCGAGGCTGAATCAGCGGGAGGCCGGCGCGCTCGACGACTGCAGAGAGCTCGCCGATTTGAATGTGGAGTTTCTGCGGTCGATTGAGAGCGTATTGGAGGCGGCGGAGGGCGTGGATGAGCAAGTGGTGGAGCGCGTCGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACGTGTATCGACGGATTGGTGGAATCGAAGAGCAGTTTGGGGAGTGCGCTATCGGCGCCGCTGTCGACTGCCGGGGAACTGTACAGCGTGTCGTTGGGATTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGCGGCAGAGTTTTCCGGGCGGCGGAGGGAACAGAGAGCCACTGATCACGCTGATCAAGGTACGCGTACGATTGAATGTGCTGCTGCTGTTTCTGTTTGTCTGTTCGTTCGGTTACAAGAACCATCGCATTTGCTTTATTCAATGCATTATAAATAAATAAATAAAAATTAGGGGAAAAAGTTTTTGTTTGGATGCAGCCATTGGTTTAGACTTCTTTTCTTAATAAAAACGAACAAAAAAAAATATAATATTTAACAATAAACAATTATTAATATTTTTTAAAAAATGAAAAAATCTATATTCTTTTTAAATAATAATGAGTCGTCAAATATTTAGTTTTCAAGTGAGACAAATATTTTCCTATTTTTAAAATCGATATTTTCACTAATGACCATGAAGTAGAAATGTCACATCATTTACAAGTAAGTTGCATAAAAATGGTATTCTTATGCAGAATGAGGAGTTTGACATTTGATAAAGACCTTTTTTTCCCCGAAATGGCTACAAATTATTGACAGAAAATTGAAATTTTATTCCTAACAAATTACTTTTATTTTGAATTAACTCAAATCTTAAAAATAAAATTATTGTCACAACACTTTAATTGCTTTCATTTTAAGAGTTATTTGAGTTATGTTCCTATAACCTAAAGTTAAGAGTAGTATGTAAGCTTATTAAGAAATTGCTTATATTCACGGGTATTTGTTTGTTTGTTTTTTTTATTTATATGTATCTACGAGTTGGTTAATCCTATATATTATACAAATATAATTTTATGACATTCATCTTTTATAATATAAATTTTTTAGCATAATTTATAATACACATCAACTCTTACTGATTATAAACCCATGAATCAAAGAAAGGATACAAATTTTCATAATAACATGATTAAATATAGACTAAACGAATTTAAGTTTTATTGAAACTTTATTATTCCAATTTTATCTTAAAAAATTGAAGAAAAAAAAAGATACATCCACACATTTAAAAAAAGATGGGTAAAACTTCCTCTTCCTTGAAGCCAACATGGCCTTAATTAATGGTTGTCATGATGGGAAATTAGTCAGGGAAAAAACAGTTGAAACGTTCAAATATTAATGGAATTAAATATCCTCCAACCATCTTAAATCAAATGGAGGGTAGATATCATATTCATAGGACTTAGGAAGTGAGAATATAATTATGTGCTGTTAACTTTTTGCATAAATTTATTAATGGGCATTTAATTGATTGCTGATTTATTTTACATTGATTATGAAAACCACCCAAAACCAATTTCTCATTATTTACAGCTAAGTTATAAAATGATAGAGCAATTTTTAACAAATGGTAAAATTCAGGATTTTTTTTTTTTTGGGTAATTTAGCCTTCATTAATTAGTTGCATTATAATATAACATGATAAAGACTTATTTTATGTGAAAATGACTAATCAGACCTCGAAATAAAAGAACTCCTCAGACAAAAGGACATGAAAGGGGTCAAAGAAGCTATTTGACTAATTACTTACAGAAGTAAAAGGAAAAAAAAAAAGATTAATAACACACTAAAATAAATCACACAAATGTTTTAATTTTTAGTGTAACAAAATTAGATTATAAAAATTTCCAATCATTTTTCTACTGAATTAGTATCAAAATTTGTATAATCTAGAAATGAAGGGAGATTGAAACTTATAATTTTATTTTATTTTTTCACTTTACCATTTAGGCTTTATCCCTGTCTACACGAAACTGCATATTTATTTATCTCTTTTAATTATATTAGTTAACAAGCAAAGTTAGGAAAGAAACCAGTTAGATTTGTGCAAATATTAATGCAGTTAAATATCCTCCAACCATATTATTTAATGTACATGTGGAGGAGAATTATCATAATAGTGGTGGAATATAATTACAAGAATCATGTACAAATTAACTTAATCAGTAATTAATTAATTTCTGATTTCTCTTAATTGGAATATCATAATAAACAAACATGGGAAGAAGGTAGGCTGCAGGGGCATCTGCAACCCTATCCTTAATACTACAAAATCCCAATTTTAAGAAATATTTTTTAGTTTATTATTAAAAAATTATTTTTTGGGGGCTGAGTTAAATATGTTTGATAAGATTCTCGGTATCACGAGAGACAGATTAGAGGGGACGGCATCTGGGATTCCCAAATTCTGCCAGTTGCATGTTACAAATTGAAATGTGGATTCTCTTCACATTTTTAAATATACATACTCTAATTATTCAATTCCTATTATTGATTTGGGTGAACTACACTTTGTCCTGATTTCGAGGTTTTGTTTCTATTTGGTTTTTAAATTTCAAAACTTGACTTTTAAAAAATAGTTCTAGAAATTTGAGGTACTATATAGCATTTGTTGATGTATTTATTTTAAATGAGGTATGTTTAAACATCAAACCGACTCAATCTAAATAAGGTTAAAAATCAAATTATTTTGGTCCTTGTATTATTAACTTTTGTCTATTTTTCTCCATGCTTTCAATTTTGATCTATTTTGGTCTTTATATATTCAATATATATATATATATATATATATATTATCCCATTCTTTCAATCATAATTTAGGCACGGTACCAAATTTGTCATATCTTTTTATTTAACGATTTTTATTGTGTAGATTTTGTATTATACAGGGATCAAAATCTTCATTTTGAACTAATTAGACAAAAGCTGGAATTATAAGTATCATAATAGAATTGAAACTTATTATGCAGTGTTTCATATATCATTATTATTCTCTTGAGTATAAATCTGTACAGTTTAGTTGATTTTAGAGGTCAATTCTCTTAAGGGTTCAGTTGCATCAGGGACTGCGAAAAATGGGGTCCTGCAACCAATCCACAATCTGCTCAGGAAGGGAGCGTTTGTTAACTGATTTAGGCACCACTGGAATTTTCATCAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGTGATGCAATTGCATTTTCTCCCAACAATTCTATGCCTGAAGATGGTTACTTTGTTATATATGTTAGAGAAGGATACTATGAAGAGTACGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCATCGGAGATGGCATTAACCGAACAGTGATCACTGGAAACCACAGCGTCGTCGATGGTTGGACGACTTATAATTCTTCTACCTTTAGTAAGTAAACCGACAAACCATCTAAATTACAAGTTTGATCCGTGAACTTGCAAGACTGTGTAACATAGATGCTTAAACTTTCAGAGTACTTGTGTCTAATAATAGGTTTACGAACTTTAACCATCTAGAAGTTTCGAAACTAAACTTGTAATTTAACCTAGTTTCGAAATATACTGAGTATTTGGATTTGGCATGTTTATGCAGCTGTTTGTGGGGATGGATTTGTTGCAATAGACGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTTGAAGGCTATCAAGACACTCTCTATGTGCATTCCTTCCGACAATTCTACAGAGAATGCGACATCTACGGCACCGTAGATTTCATATTCGGCAATGCCGCTGCGGTTTTCCAGCAATGCAACCTCTATGCCAGAAAGCCCCTACCGAACCAAAAGAATGCCTTCACAGCGCAGGGTCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCACAACTGCACGATCAGAGCTGCACCCGACTGGGCAATGGACTCGAACGCGACGATAGCGAACTACCTCGGGCGGCCATGGAAGCAGTACTCAAGGACAGTGTACATGCAGTCATACATTGGGGATTTGATCAGCCCTGTGGGATGGCTGGAGTGGAATGGAACAGCAGGGCTGGAGACATTGTATTATGGGGAGTTTGAGAATCATGGGCCAGGAGCAAACACAAGTATGAGGGTGAATTGGCCTGGCTATAGCTTGATGAATGCTACACAGGCCATGAATTTTACTGTCTATAACTTCACTATGGGAGATACTTGGCTGCCTTATACTGATATCCCCTTCAATGGAGGTCTGGTATGA

mRNA sequence

ATGAACTCACTTCCCTACATTTTTCTTCTCCTTCTCTCCCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCTTCCGCCGCGCTCTGTAAAACCACTCTCTACCCTAAGCTCTGCCATTCGATTCTCTCCTCCATCCGATTCTCGCCCTCCGATCCCTACGGCTACGGAAAATTCTCCGTCAAACAGTGTCTGAAGCAGGCGATCAAAATGTCGAGCGTAATCGGCGGCTACCTTCGCCGTGGAGGCGGTTCGAGGCTGAATCAGCGGGAGGCCGGCGCGCTCGACGACTGCAGAGAGCTCGCCGATTTGAATGTGGAGTTTCTGCGGTCGATTGAGAGCGTATTGGAGGCGGCGGAGGGCGTGGATGAGCAAGTGGTGGAGCGCGTCGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACGTGTATCGACGGATTGGTGGAATCGAAGAGCAGTTTGGGGAGTGCGCTATCGGCGCCGCTGTCGACTGCCGGGGAACTGTACAGCGTGTCGTTGGGATTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGCGGCAGAGTTTTCCGGGCGGCGGAGGGAACAGAGAGCCACTGATCACGCTGATCAAGGGACTGCGAAAAATGGGGTCCTGCAACCAATCCACAATCTGCTCAGGAAGGGAGCGTTTGTTAACTGATTTAGGCACCACTGGAATTTTCATCAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGTGATGCAATTGCATTTTCTCCCAACAATTCTATGCCTGAAGATGGTTACTTTGTTATATATGTTAGAGAAGGATACTATGAAGAGTACGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCATCGGAGATGGCATTAACCGAACAGTGATCACTGGAAACCACAGCGTCGTCGATGGTTGGACGACTTATAATTCTTCTACCTTTACTGTTTGTGGGGATGGATTTGTTGCAATAGACGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTTGAAGGCTATCAAGACACTCTCTATGTGCATTCCTTCCGACAATTCTACAGAGAATGCGACATCTACGGCACCGTAGATTTCATATTCGGCAATGCCGCTGCGGTTTTCCAGCAATGCAACCTCTATGCCAGAAAGCCCCTACCGAACCAAAAGAATGCCTTCACAGCGCAGGGTCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCACAACTGCACGATCAGAGCTGCACCCGACTGGGCAATGGACTCGAACGCGACGATAGCGAACTACCTCGGGCGGCCATGGAAGCAGTACTCAAGGACAGTGTACATGCAGTCATACATTGGGGATTTGATCAGCCCTGTGGGATGGCTGGAGTGGAATGGAACAGCAGGGCTGGAGACATTGTATTATGGGGAGTTTGAGAATCATGGGCCAGGAGCAAACACAAGTATGAGGGTGAATTGGCCTGGCTATAGCTTGATGAATGCTACACAGGCCATGAATTTTACTGTCTATAACTTCACTATGGGAGATACTTGGCTGCCTTATACTGATATCCCCTTCAATGGAGGTCTGGTATGA

Coding sequence (CDS)

ATGAACTCACTTCCCTACATTTTTCTTCTCCTTCTCTCCCTCCGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCTTCCGCCGCGCTCTGTAAAACCACTCTCTACCCTAAGCTCTGCCATTCGATTCTCTCCTCCATCCGATTCTCGCCCTCCGATCCCTACGGCTACGGAAAATTCTCCGTCAAACAGTGTCTGAAGCAGGCGATCAAAATGTCGAGCGTAATCGGCGGCTACCTTCGCCGTGGAGGCGGTTCGAGGCTGAATCAGCGGGAGGCCGGCGCGCTCGACGACTGCAGAGAGCTCGCCGATTTGAATGTGGAGTTTCTGCGGTCGATTGAGAGCGTATTGGAGGCGGCGGAGGGCGTGGATGAGCAAGTGGTGGAGCGCGTCGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACGTGTATCGACGGATTGGTGGAATCGAAGAGCAGTTTGGGGAGTGCGCTATCGGCGCCGCTGTCGACTGCCGGGGAACTGTACAGCGTGTCGTTGGGATTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGCGGCAGAGTTTTCCGGGCGGCGGAGGGAACAGAGAGCCACTGATCACGCTGATCAAGGGACTGCGAAAAATGGGGTCCTGCAACCAATCCACAATCTGCTCAGGAAGGGAGCGTTTGTTAACTGATTTAGGCACCACTGGAATTTTCATCAACAACACTGTGGTTGTTAGCTCAACTGGTGCTGACAACTTCACATCAATTGGTGATGCAATTGCATTTTCTCCCAACAATTCTATGCCTGAAGATGGTTACTTTGTTATATATGTTAGAGAAGGATACTATGAAGAGTACGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCATCGGAGATGGCATTAACCGAACAGTGATCACTGGAAACCACAGCGTCGTCGATGGTTGGACGACTTATAATTCTTCTACCTTTACTGTTTGTGGGGATGGATTTGTTGCAATAGACGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTTGAAGGCTATCAAGACACTCTCTATGTGCATTCCTTCCGACAATTCTACAGAGAATGCGACATCTACGGCACCGTAGATTTCATATTCGGCAATGCCGCTGCGGTTTTCCAGCAATGCAACCTCTATGCCAGAAAGCCCCTACCGAACCAAAAGAATGCCTTCACAGCGCAGGGTCGAACCGATCCGAACCAAAGCACAGGCATCTCAATCCACAACTGCACGATCAGAGCTGCACCCGACTGGGCAATGGACTCGAACGCGACGATAGCGAACTACCTCGGGCGGCCATGGAAGCAGTACTCAAGGACAGTGTACATGCAGTCATACATTGGGGATTTGATCAGCCCTGTGGGATGGCTGGAGTGGAATGGAACAGCAGGGCTGGAGACATTGTATTATGGGGAGTTTGAGAATCATGGGCCAGGAGCAAACACAAGTATGAGGGTGAATTGGCCTGGCTATAGCTTGATGAATGCTACACAGGCCATGAATTTTACTGTCTATAACTTCACTATGGGAGATACTTGGCTGCCTTATACTGATATCCCCTTCAATGGAGGTCTGGTATGA

Protein sequence

MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAEGVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNSLSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPFNGGLV
Homology
BLAST of Moc09g37040 vs. NCBI nr
Match: XP_022150485.1 (probable pectinesterase/pectinesterase inhibitor 25 [Momordica charantia])

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINN 240
           LSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINN
Sbjct: 181 LSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINN 240

Query: 241 TVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGD 300
           TVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGD
Sbjct: 241 TVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGD 300

Query: 301 GINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLS 360
           GINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLS
Sbjct: 301 GINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLS 360

Query: 361 TFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNA 420
           TFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNA
Sbjct: 361 TFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNA 420

Query: 421 FTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDL 480
           FTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDL
Sbjct: 421 FTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDL 480

Query: 481 ISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMG 540
           ISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMG
Sbjct: 481 ISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMG 540

Query: 541 DTWLPYTDIPFNGGLV 557
           DTWLPYTDIPFNGGLV
Sbjct: 541 DTWLPYTDIPFNGGLV 556

BLAST of Moc09g37040 vs. NCBI nr
Match: XP_008446714.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo])

HSP 1 Score: 998.8 bits (2581), Expect = 1.8e-287
Identity = 491/563 (87.21%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNSLP+IFLLLLSL  AGAS SPP S ALCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQC+KQAIKMS+VIG YL RG  SRLN+REAGAL DCREL+DLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE++VERVES LSAIVTNGQTC+DGLVES+SSLG ALSAPL +AGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQ------SFPGGGGNREPLITLIKGLRKMGSC-NQSTICSGRERLLTDLGT 240
           +SRRWKKRR+        PGGG +RE L TLIKGL KM  C N ST CSGR+RLLTDLG+
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300
           +GI INNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVREGYYEEYVVVPK+KT
Sbjct: 241 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRT+ITGNH+VVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA+
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTI+AAPDW MDSN T  NYLGRPWKQYSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540
           QSYIGDLISPVGWLEWNGT GLETLYYGE+EN+GPGANTS+RVNWPG+SLMN TQAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFNGGLV 557
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. NCBI nr
Match: XP_004135375.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus] >KGN52152.1 hypothetical protein Csa_007825 [Cucumis sativus])

HSP 1 Score: 997.7 bits (2578), Expect = 4.1e-287
Identity = 488/563 (86.68%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNSLP+IFLLLLSL  AGAS SPPPS ALCK+TLYPKLC SILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQC+KQA KMS+VIG YL RG  SRLN+ EAGAL DCR+L+DLNVEFLRSIE VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE++VERVESILSAIVTNGQTCIDGLVES+SSLG+ALS PL +AGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQ------SFPGGGGNREPLITLIKGLRKMGSC-NQSTICSGRERLLTDLGT 240
           +SRRWKKRR+        PGGG +REPL TLIKGL KM  C NQST C GR+RLLTDLG+
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLGS 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYV+EGYYEEYVVVPK+KT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRT+ITGNH+VVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA+
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTI+AAPDW MDSN T  NYLGRPWKQYSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540
           QSYIGDLISPVGWLEWNGT GLETLYYGE+EN+GPGANTS+RVNWPG+SL+N TQAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFNGGLV 557
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. NCBI nr
Match: XP_038891461.1 (probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida])

HSP 1 Score: 991.1 bits (2561), Expect = 3.8e-285
Identity = 491/564 (87.06%), Postives = 518/564 (91.84%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNS+P+IFL LL L  AGA DSPPPS ALCK+TLYPKLC SILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLFLLFLGFAGAYDSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQCLKQAIKMSSVIG YL RG  SRLN+REAGAL DC+EL+D NVEFLRSIE VL+AAE
Sbjct: 61  VKQCLKQAIKMSSVIGHYLDRGRDSRLNRREAGALSDCKELSDSNVEFLRSIEGVLDAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE+ VERVESILSAIVTNGQTCIDGLVES SS G+ LSAPLS AGELYSVSLGLV NS
Sbjct: 121 GVDEERVERVESILSAIVTNGQTCIDGLVESGSSFGNVLSAPLSIAGELYSVSLGLVSNS 180

Query: 181 LSRRWKKRRQ-------SFPGGGGNREPLITLIKGLRKMGSC-NQSTICSGRERLLTDLG 240
           LSRRWKKRRQ       +FP G  NREPL TLIKGL KMGSC NQS  CSGR+RLLT+LG
Sbjct: 181 LSRRWKKRRQKGNGDGGNFPAGPRNREPLNTLIKGLHKMGSCNNQSRKCSGRKRLLTNLG 240

Query: 241 TTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYK 300
           +TGI INNTVVVSSTG DNFTSIGDAIAF+PNNSMP+DGYFVIYVREGYYEEYVV+PK+K
Sbjct: 241 STGILINNTVVVSSTGGDNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVIPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360
           TN+MLIGDGINRTVITGN +V+DGWTTYNSSTFTVCGDGFVAID+TFRNTAGPEKHQAVA
Sbjct: 301 TNVMLIGDGINRTVITGNRNVIDGWTTYNSSTFTVCGDGFVAIDMTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420
           VRNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 VRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTI AAPDW MDSN T  NYLGRPWKQYSRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIMAAPDWVMDSNTT-TNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNF 540
           MQSYIGDLISPVGWLEWNGT GLETLYYGEFEN+GPGANTS+RVNWPGYSLMN TQAMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEFENYGPGANTSLRVNWPGYSLMNLTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFNGGLV 557
           TVYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. NCBI nr
Match: XP_022976564.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 988.8 bits (2555), Expect = 1.9e-284
Identity = 483/563 (85.79%), Postives = 522/563 (92.72%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNS P+IFL LLSLRLAGASDSPPPSA LCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           V++CLKQAIKMSSVIG YL RGG SRLN++E GAL+DCREL+D NVEFL+SI+ VLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE+++ER+ESILSAIVTNGQTCIDGLVES+SSLG+ALSAPL +AGELYSVSLGLVKNS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQ-------SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGT 240
           LSRRWKKR Q       +FP    +REPL TLIKGL  MGSCNQST CSGR+R+LTDLGT
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+F+PNNSMP+DGYFVIYVR+GYY+EYVVVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECD+YGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP+W MD N T  NYLGRPWK +SRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540
           QSYIGDLISPVGWLEWNGT GL+TLYYGEFEN+GPGANTS+RV+W G+SLMN T+AMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFNGGLV 557
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 8.5e-195
Identity = 337/547 (61.61%), Postives = 418/547 (76.42%), Query Frame = 0

Query: 20  SDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCLKQAIKMSSVIGGYL 79
           S S  PS A CK+T YPKLC +IL++++ SPSDPY YGKF++KQCLKQA ++S VI  Y 
Sbjct: 74  SQSQSPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  RR--GGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAEGVDEQVVERVESILSAI 139
           RR           E GA+ DC EL++L+V +L ++ + L+ A+ +   +VE V S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNSLSR---RWKKRRQSFPG 199
           VTN QTC+DGLVE+KS   +A+ +P+     LYS+SLGLV ++L+R   R+K  +    G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GGGN--REPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTT---GIFINNTVVVSSTGA 259
           GG +  REPL TLIKGLRK  +C+    C    R L +LG T    I ++  V+V    +
Sbjct: 254 GGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 260 DNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGDGINRTVITG 319
           DNFT+I DAIA +PNN+ PEDGYFVIY REG YEEY+VVP  K N+ML+GDGIN+T+ITG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 320 NHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEG 379
           NH+VVDGWTTYN S+F V G+ F+A+DVTFRNTAGPEKHQAVA+RN+A+ S+FYRCSFEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 380 YQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDP 439
           YQDTLYVHS RQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR DP
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 440 NQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEW 499
           NQ+TGISI NCTI+AAPD A +  + +  +LGRPWK YSRTV+MQSYI D++ PVGWLEW
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 553

Query: 500 NGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDI 557
           NGT GL+T+YYGE+ N GPGANT+ RV W GY+L+N  +AMNFTVYNFTMGDTWLP TDI
Sbjct: 554 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 613

BLAST of Moc09g37040 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 8.0e-193
Identity = 340/545 (62.39%), Postives = 409/545 (75.05%), Query Frame = 0

Query: 22  SPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCLKQAIKMSSVIGGYLRR 81
           SP P    CK+T YPKLC +ILS+++ SPSDPY YGKF++KQCLKQA ++S VI  + +R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAEGVDEQVVERVESILSAIVT 141
                      E  A+ DC ELA+L+VE+L ++   L+AAE +   +V+RV S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNSLSRRWKKRRQSFPG--GGG 201
           N QTC+DGLV++KS   +A+  PL     LYSVSLGLV ++L+R  K+ + S     GGG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 N---REPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTT---GIFINNTVVVSSTGADN 261
           N   REPL TLIK LRK  +C++   C    R L +LG T    I +   V V     DN
Sbjct: 260 NKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDN 319

Query: 262 FTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGDGINRTVITGNH 321
           F +I +A+A +PN++ PE GYFVIY R G YEEYVV+   K NIMLIGDGIN+T+I+GNH
Sbjct: 320 FPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNH 379

Query: 322 SVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQ 381
           S +DGWTTYNSSTF V GD FVA+DVTFRNTAGPEKHQAVAVRN+AD STFYRCSFEGYQ
Sbjct: 380 SFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQ 439

Query: 382 DTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ 441
           DTLYVHS RQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRTDPNQ
Sbjct: 440 DTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQ 499

Query: 442 STGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNG 501
            TGISI NCTI AAPD A D  +T+  +LGRPWK YSRTVY+QSYI D++ PVGWLEWNG
Sbjct: 500 KTGISIINCTIGAAPDLAADPKSTM-TFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNG 559

Query: 502 TAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPF 557
           T GL+T+ YGE++N GPGA+TS RV W GYSL+N  QAMNFTVYNFT+GDTWLP TDIPF
Sbjct: 560 TTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPF 619

BLAST of Moc09g37040 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 506.1 bits (1302), Expect = 4.9e-142
Identity = 277/566 (48.94%), Postives = 369/566 (65.19%), Query Frame = 0

Query: 7   IFLLLLSLRLAGASDSPP-PSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCL 66
           +FL L +L +A  +  P   S+ +CKTT  PK C S+    + S  D   YG+FS+++ L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFL-RSIESV--LEAAEGV 126
            Q+ K +  I  YL+R   + L+Q   GAL DCR LA L  ++L  S E+V    +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEQVVERVESILSAIVTNGQTCIDGLVESKSS---LGSALSAPLSTAGELYSVSLGLVKN 186
                + ++++LSA +TN QTC+DG+  + SS   + + ++ PL    +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTK 190

Query: 187 SLSRRWKKRRQSF-----PGGGGNREPLITLIKGL--RKMGSCNQSTICSGRERLLT--D 246
               + KK+  S+          + +P      G    KM    ++   S   R L   D
Sbjct: 191 GWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLADDD 250

Query: 247 LGTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPK 306
                + +++ V V+  G  NFT+I +A+  +PN +    GYFVIYV  G YEE VV+ K
Sbjct: 251 NDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAK 310

Query: 307 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQA 366
            K  +M+IGDGINRTV+TGN +VVDGWTT+NS+TF V    FVA+++TFRNTAGPEKHQA
Sbjct: 311 NKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQA 370

Query: 367 VAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYA 426
           VA+R+SADLS FY CSFE YQDTLY HS RQFYRECDIYGTVDFIFGNAA VFQ CNLY 
Sbjct: 371 VAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYP 430

Query: 427 RKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRT 486
           R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTI+ A D  + SN T+  YLGRPWK+YSRT
Sbjct: 431 RQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADD-LVSSNYTVKTYLGRPWKEYSRT 490

Query: 487 VYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAM 546
           V+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV WPGY ++N+T A 
Sbjct: 491 VFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDAN 550

Query: 547 NFTVYNFTMGDTWLPYTDIPFNGGLV 557
           NFTV NF +GD W+  + +P+  GL+
Sbjct: 551 NFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Moc09g37040 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.5e-135
Identity = 271/581 (46.64%), Postives = 358/581 (61.62%), Query Frame = 0

Query: 5   PYIFLLLLSLRL---AGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSV 64
           P   L+ LS  L     +S +   S+ +CKTT  PK C S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSI 63

Query: 65  KQCLKQAIKMSSVIGGYLRRGGGSRLNQREA-GALDDCRELADLNVEF-LRSIESVLEAA 124
           ++ L Q+ K    +  Y++R   + L+Q     AL DCR LA L +++ L S E+V + +
Sbjct: 64  RKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 125 -----EGVDEQVVERVESILSAIVTNGQTCIDGLVESKS-----SLGSALSAPLSTAGEL 184
                + +     + ++++LSA +TN QTC++GL  + S     ++ + ++ PL    +L
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 185 YSVSLGLVKNSLSRRWKKR-----RQSFPGGGGNREPLITLIKGL--RKMGSCNQSTICS 244
             VSL L       + KKR      Q   G   + +P      G    KM    ++   S
Sbjct: 184 LGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYES 243

Query: 245 GRERLLTDLGTTG-------IFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVI 304
              R L D  + G       + I++ V VS  G  NFT+I  A+A +PNN+    G+F+I
Sbjct: 244 LSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 303

Query: 305 YVREGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAI 364
           YV  G YEEY+ + K K  +M+IGDGIN+TV+TGN SVVDGWTT+NS+TF V    FVA+
Sbjct: 304 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 363

Query: 365 DVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFI 424
           ++TFRNTAGPEKHQAVA+R+ AD S FY CSFE YQDTLY HS RQFYRECD+YGTVDFI
Sbjct: 364 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 423

Query: 425 FGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNAT 484
           FGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTI+ A D  + SN T
Sbjct: 424 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADD-LVSSNYT 483

Query: 485 IANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMR 544
           +  YLGRPWK+YSRTVYMQSYI   + PVGW EWNG   L TLYY E+ N GPG+NT+ R
Sbjct: 484 VKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNR 543

Query: 545 VNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPFNGGLV 557
           V WPGY ++N+T A NFTV    +   W+  T +P+  GL+
Sbjct: 544 VTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Moc09g37040 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 482.6 bits (1241), Expect = 5.8e-135
Identity = 266/569 (46.75%), Postives = 346/569 (60.81%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           ++SLP  F   +S ++    ++  PS  +C+    P  C S+L +    P D Y YG+ S
Sbjct: 14  LSSLPSPF---ISAQIPAIGNATSPS-NICRFAPDPSYCRSVLPN---QPGDIYSYGRLS 73

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           +++ L +A +  S+I   L R  G    +   GAL+DC+ LA L +++L S     ++ +
Sbjct: 74  LRRSLSRARRFISMIDAELDR-KGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTK 133

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
            +     E V + LSA +TN QTC++GL  + S  G  LS  L    +LY VSL L    
Sbjct: 134 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENG--LSGDLFNDTKLYGVSLAL---- 193

Query: 181 LSRRWKKRRQ-------------SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERL 240
            S+ W  RRQ              F G    + PL        KM    ++   +   R 
Sbjct: 194 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPL--------KMTERARAVYNTVTRRK 253

Query: 241 LTDLGTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVV 300
           L       + +++ V V   G  NFT+I  AIA +PN +   +GYF+IYV  G YEEYV 
Sbjct: 254 LLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 313

Query: 301 VPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEK 360
           VPK K  +M+IGDGIN+TVITGN SVVDGWTT+NS+TF + G  F+ +++T RNTAGP K
Sbjct: 314 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 373

Query: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCN 420
            QAVA+R+  DLS FY CSFE YQDTLY HS RQFYRECD+YGTVDFIFGNAA V Q CN
Sbjct: 374 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 433

Query: 421 LYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQY 480
           LY R+P   Q N  TAQGRTDPNQ+TG +IH CTIR A D A  SN T+  YLGRPWK+Y
Sbjct: 434 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEY 493

Query: 481 SRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNAT 540
           SRTV MQ+YI   + P GW  W+G   L TLYY E+ N GPG++T+ RV WPGY ++NAT
Sbjct: 494 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 553

Query: 541 QAMNFTVYNFTMGDTWLPYTDIPFNGGLV 557
            A NFTV NF +G+ W+  T +PF GGL+
Sbjct: 554 DASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Moc09g37040 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINN 240
           LSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINN
Sbjct: 181 LSRRWKKRRQSFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTTGIFINN 240

Query: 241 TVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGD 300
           TVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGD
Sbjct: 241 TVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGD 300

Query: 301 GINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLS 360
           GINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLS
Sbjct: 301 GINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLS 360

Query: 361 TFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNA 420
           TFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNA
Sbjct: 361 TFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNA 420

Query: 421 FTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDL 480
           FTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDL
Sbjct: 421 FTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDL 480

Query: 481 ISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMG 540
           ISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMG
Sbjct: 481 ISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMG 540

Query: 541 DTWLPYTDIPFNGGLV 557
           DTWLPYTDIPFNGGLV
Sbjct: 541 DTWLPYTDIPFNGGLV 556

BLAST of Moc09g37040 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 8.9e-288
Identity = 491/563 (87.21%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNSLP+IFLLLLSL  AGAS SPP S ALCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQC+KQAIKMS+VIG YL RG  SRLN+REAGAL DCREL+DLNVEFLRSIESVLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE++VERVES LSAIVTNGQTC+DGLVES+SSLG ALSAPL +AGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQ------SFPGGGGNREPLITLIKGLRKMGSC-NQSTICSGRERLLTDLGT 240
           +SRRWKKRR+        PGGG +RE L TLIKGL KM  C N ST CSGR+RLLTDLG+
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLGS 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300
           +GI INNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVREGYYEEYVVVPK+KT
Sbjct: 241 SGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRT+ITGNH+VVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA+
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTI+AAPDW MDSN T  NYLGRPWKQYSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540
           QSYIGDLISPVGWLEWNGT GLETLYYGE+EN+GPGANTS+RVNWPG+SLMN TQAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFNGGLV 557
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. ExPASy TrEMBL
Match: A0A0A0KUN8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 488/563 (86.68%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNSLP+IFLLLLSL  AGAS SPPPS ALCK+TLYPKLC SILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           VKQC+KQA KMS+VIG YL RG  SRLN+ EAGAL DCR+L+DLNVEFLRSIE VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE++VERVESILSAIVTNGQTCIDGLVES+SSLG+ALS PL +AGELYSVSLGLV N+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRRQ------SFPGGGGNREPLITLIKGLRKMGSC-NQSTICSGRERLLTDLGT 240
           +SRRWKKRR+        PGGG +REPL TLIKGL KM  C NQST C GR+RLLTDLG+
Sbjct: 181 MSRRWKKRREKGGGNGGVPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLGS 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYV+EGYYEEYVVVPK+KT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRT+ITGNH+VVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA+
Sbjct: 301 NIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAL 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480
           LPNQKNAFTAQGRTDPNQ+TGISIHNCTI+AAPDW MDSN T  NYLGRPWKQYSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540
           QSYIGDLISPVGWLEWNGT GLETLYYGE+EN+GPGANTS+RVNWPG+SL+N TQAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFNGGLV 557
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 9.2e-285
Identity = 483/563 (85.79%), Postives = 522/563 (92.72%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           MNS P+IFL LLSLRLAGASDSPPPSA LCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           V++CLKQAIKMSSVIG YL RGG SRLN++E GAL+DCREL+D NVEFL+SI+ VLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
           GVDE+++ER+ESILSAIVTNGQTCIDGLVES+SSLG+ALSAPL +AGELYSVSLGLVKNS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRRQ-------SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGT 240
           LSRRWKKR Q       +FP    +REPL TLIKGL  MGSCNQST CSGR+R+LTDLGT
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+F+PNNSMP+DGYFVIYVR+GYY+EYVVVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360
           NIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECD+YGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP+W MD N T  NYLGRPWK +SRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540
           QSYIGDLISPVGWLEWNGT GL+TLYYGEFEN+GPGANTS+RV+W G+SLMN T+AMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFNGGLV 557
           VYNFTMGDTWLPYTDIPF+GGL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Moc09g37040 vs. ExPASy TrEMBL
Match: A0A6J1HU46 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.6e-281
Identity = 491/565 (86.90%), Postives = 521/565 (92.21%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDS---PPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYG 60
           MNS+  IFLLLLSLR AGA DS   PPPSA LCK+TLYPKLC SILSSIRFSPSDPYGYG
Sbjct: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLE 120
           KFSVK CLKQAIKMSSVIG YL RGG SRLNQREAGAL+DCR+L+D NVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAEGVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLV 180
           AA+GVDE++VERV+SILSAIVTNGQTCIDGLVES+SSLG+AL+AP+S+AG+LYSVSLGLV
Sbjct: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 KNSLSRRWKKRRQSFPGGGG-----NREPLITLIKGLRKMGSCNQSTICSGRERLLTDLG 240
            NSLSR WKKRR    GG       NREPL TLIKGL KMGSCNQST CSGR+RLL DLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYK 300
           TTGIFIN+TVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVR GYYEEYVVVPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300

Query: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360
           TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGD FVAIDVTFRNTAGPEKHQAVA
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420
           +R+SADLSTFYRCSFEGYQDTLYVHS RQFYRECDIYGTVDFIFGNAAAVFQ+CNLYARK
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMD-SNATIANYLGRPWKQYSRTV 480
           PLPNQK A TAQGRTDPNQ+TGISIH+CTI+AAPDW  D SN+T  NYLGRPWKQYSRTV
Sbjct: 421 PLPNQKIAITAQGRTDPNQNTGISIHDCTIKAAPDWETDYSNSTTTNYLGRPWKQYSRTV 480

Query: 481 YMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMN 540
           YMQ YIGDLISPVGWLEWNGTAGL+TLYYGEFEN+GPGANTSMRV+WPGYSLMN TQAMN
Sbjct: 481 YMQCYIGDLISPVGWLEWNGTAGLDTLYYGEFENYGPGANTSMRVDWPGYSLMNITQAMN 540

Query: 541 FTVYNFTMGDTWLPYTDIPFNGGLV 557
           FTVYN TMGDTWLPYTDIPF+GGLV
Sbjct: 541 FTVYNLTMGDTWLPYTDIPFSGGLV 564

BLAST of Moc09g37040 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 681.4 bits (1757), Expect = 6.1e-196
Identity = 337/547 (61.61%), Postives = 418/547 (76.42%), Query Frame = 0

Query: 20  SDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCLKQAIKMSSVIGGYL 79
           S S  PS A CK+T YPKLC +IL++++ SPSDPY YGKF++KQCLKQA ++S VI  Y 
Sbjct: 74  SQSQSPSLA-CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  RR--GGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAEGVDEQVVERVESILSAI 139
           RR           E GA+ DC EL++L+V +L ++ + L+ A+ +   +VE V S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNSLSR---RWKKRRQSFPG 199
           VTN QTC+DGLVE+KS   +A+ +P+     LYS+SLGLV ++L+R   R+K  +    G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GGGN--REPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTT---GIFINNTVVVSSTGA 259
           GG +  REPL TLIKGLRK  +C+    C    R L +LG T    I ++  V+V    +
Sbjct: 254 GGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 260 DNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGDGINRTVITG 319
           DNFT+I DAIA +PNN+ PEDGYFVIY REG YEEY+VVP  K N+ML+GDGIN+T+ITG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 320 NHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEG 379
           NH+VVDGWTTYN S+F V G+ F+A+DVTFRNTAGPEKHQAVA+RN+A+ S+FYRCSFEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 380 YQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDP 439
           YQDTLYVHS RQFYRECDIYGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR DP
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 440 NQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEW 499
           NQ+TGISI NCTI+AAPD A +  + +  +LGRPWK YSRTV+MQSYI D++ PVGWLEW
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 553

Query: 500 NGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDI 557
           NGT GL+T+YYGE+ N GPGANT+ RV W GY+L+N  +AMNFTVYNFTMGDTWLP TDI
Sbjct: 554 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDI 613

BLAST of Moc09g37040 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 674.9 bits (1740), Expect = 5.7e-194
Identity = 340/545 (62.39%), Postives = 409/545 (75.05%), Query Frame = 0

Query: 22  SPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCLKQAIKMSSVIGGYLRR 81
           SP P    CK+T YPKLC +ILS+++ SPSDPY YGKF++KQCLKQA ++S VI  + +R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAEGVDEQVVERVESILSAIVT 141
                      E  A+ DC ELA+L+VE+L ++   L+AAE +   +V+RV S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNSLSRRWKKRRQSFPG--GGG 201
           N QTC+DGLV++KS   +A+  PL     LYSVSLGLV ++L+R  K+ + S     GGG
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 N---REPLITLIKGLRKMGSCNQSTICSGRERLLTDLGTT---GIFINNTVVVSSTGADN 261
           N   REPL TLIK LRK  +C++   C    R L +LG T    I +   V V     DN
Sbjct: 260 NKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDN 319

Query: 262 FTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKTNIMLIGDGINRTVITGNH 321
           F +I +A+A +PN++ PE GYFVIY R G YEEYVV+   K NIMLIGDGIN+T+I+GNH
Sbjct: 320 FPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNH 379

Query: 322 SVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQ 381
           S +DGWTTYNSSTF V GD FVA+DVTFRNTAGPEKHQAVAVRN+AD STFYRCSFEGYQ
Sbjct: 380 SFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQ 439

Query: 382 DTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQ 441
           DTLYVHS RQFYRECDIYGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GRTDPNQ
Sbjct: 440 DTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQ 499

Query: 442 STGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNG 501
            TGISI NCTI AAPD A D  +T+  +LGRPWK YSRTVY+QSYI D++ PVGWLEWNG
Sbjct: 500 KTGISIINCTIGAAPDLAADPKSTM-TFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNG 559

Query: 502 TAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPF 557
           T GL+T+ YGE++N GPGA+TS RV W GYSL+N  QAMNFTVYNFT+GDTWLP TDIPF
Sbjct: 560 TTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPF 619

BLAST of Moc09g37040 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 506.1 bits (1302), Expect = 3.5e-143
Identity = 277/566 (48.94%), Postives = 369/566 (65.19%), Query Frame = 0

Query: 7   IFLLLLSLRLAGASDSPP-PSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSVKQCL 66
           +FL L +L +A  +  P   S+ +CKTT  PK C S+    + S  D   YG+FS+++ L
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSLRKSL 70

Query: 67  KQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFL-RSIESV--LEAAEGV 126
            Q+ K +  I  YL+R   + L+Q   GAL DCR LA L  ++L  S E+V    +++ +
Sbjct: 71  TQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 130

Query: 127 DEQVVERVESILSAIVTNGQTCIDGLVESKSS---LGSALSAPLSTAGELYSVSLGLVKN 186
                + ++++LSA +TN QTC+DG+  + SS   + + ++ PL    +L+SVSL L   
Sbjct: 131 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTK 190

Query: 187 SLSRRWKKRRQSF-----PGGGGNREPLITLIKGL--RKMGSCNQSTICSGRERLLT--D 246
               + KK+  S+          + +P      G    KM    ++   S   R L   D
Sbjct: 191 GWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLADDD 250

Query: 247 LGTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPK 306
                + +++ V V+  G  NFT+I +A+  +PN +    GYFVIYV  G YEE VV+ K
Sbjct: 251 NDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAK 310

Query: 307 YKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQA 366
            K  +M+IGDGINRTV+TGN +VVDGWTT+NS+TF V    FVA+++TFRNTAGPEKHQA
Sbjct: 311 NKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQA 370

Query: 367 VAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYA 426
           VA+R+SADLS FY CSFE YQDTLY HS RQFYRECDIYGTVDFIFGNAA VFQ CNLY 
Sbjct: 371 VAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYP 430

Query: 427 RKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRT 486
           R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTI+ A D  + SN T+  YLGRPWK+YSRT
Sbjct: 431 RQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADD-LVSSNYTVKTYLGRPWKEYSRT 490

Query: 487 VYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAM 546
           V+MQSYI +++ PVGW EWNG   L TLYY E+ N G G++T+ RV WPGY ++N+T A 
Sbjct: 491 VFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDAN 550

Query: 547 NFTVYNFTMGDTWLPYTDIPFNGGLV 557
           NFTV NF +GD W+  + +P+  GL+
Sbjct: 551 NFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Moc09g37040 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 484.6 bits (1246), Expect = 1.1e-136
Identity = 271/581 (46.64%), Postives = 358/581 (61.62%), Query Frame = 0

Query: 5   PYIFLLLLSLRL---AGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFSV 64
           P   L+ LS  L     +S +   S+ +CKTT  PK C S+      S  +   YG FS+
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSI 63

Query: 65  KQCLKQAIKMSSVIGGYLRRGGGSRLNQREA-GALDDCRELADLNVEF-LRSIESVLEAA 124
           ++ L Q+ K    +  Y++R   + L+Q     AL DCR LA L +++ L S E+V + +
Sbjct: 64  RKSLSQSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTS 123

Query: 125 -----EGVDEQVVERVESILSAIVTNGQTCIDGLVESKS-----SLGSALSAPLSTAGEL 184
                + +     + ++++LSA +TN QTC++GL  + S     ++ + ++ PL    +L
Sbjct: 124 AKTSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKL 183

Query: 185 YSVSLGLVKNSLSRRWKKR-----RQSFPGGGGNREPLITLIKGL--RKMGSCNQSTICS 244
             VSL L       + KKR      Q   G   + +P      G    KM    ++   S
Sbjct: 184 LGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYES 243

Query: 245 GRERLLTDLGTTG-------IFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVI 304
              R L D  + G       + I++ V VS  G  NFT+I  A+A +PNN+    G+F+I
Sbjct: 244 LSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 303

Query: 305 YVREGYYEEYVVVPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAI 364
           YV  G YEEY+ + K K  +M+IGDGIN+TV+TGN SVVDGWTT+NS+TF V    FVA+
Sbjct: 304 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 363

Query: 365 DVTFRNTAGPEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFI 424
           ++TFRNTAGPEKHQAVA+R+ AD S FY CSFE YQDTLY HS RQFYRECD+YGTVDFI
Sbjct: 364 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 423

Query: 425 FGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNAT 484
           FGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTI+ A D  + SN T
Sbjct: 424 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADD-LVSSNYT 483

Query: 485 IANYLGRPWKQYSRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMR 544
           +  YLGRPWK+YSRTVYMQSYI   + PVGW EWNG   L TLYY E+ N GPG+NT+ R
Sbjct: 484 VKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNR 543

Query: 545 VNWPGYSLMNATQAMNFTVYNFTMGDTWLPYTDIPFNGGLV 557
           V WPGY ++N+T A NFTV    +   W+  T +P+  GL+
Sbjct: 544 VTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Moc09g37040 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 482.6 bits (1241), Expect = 4.1e-136
Identity = 266/569 (46.75%), Postives = 346/569 (60.81%), Query Frame = 0

Query: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60
           ++SLP  F   +S ++    ++  PS  +C+    P  C S+L +    P D Y YG+ S
Sbjct: 14  LSSLPSPF---ISAQIPAIGNATSPS-NICRFAPDPSYCRSVLPN---QPGDIYSYGRLS 73

Query: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120
           +++ L +A +  S+I   L R  G    +   GAL+DC+ LA L +++L S     ++ +
Sbjct: 74  LRRSLSRARRFISMIDAELDR-KGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTK 133

Query: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180
            +     E V + LSA +TN QTC++GL  + S  G  LS  L    +LY VSL L    
Sbjct: 134 TLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENG--LSGDLFNDTKLYGVSLAL---- 193

Query: 181 LSRRWKKRRQ-------------SFPGGGGNREPLITLIKGLRKMGSCNQSTICSGRERL 240
            S+ W  RRQ              F G    + PL        KM    ++   +   R 
Sbjct: 194 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPL--------KMTERARAVYNTVTRRK 253

Query: 241 LTDLGTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVV 300
           L       + +++ V V   G  NFT+I  AIA +PN +   +GYF+IYV  G YEEYV 
Sbjct: 254 LLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 313

Query: 301 VPKYKTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEK 360
           VPK K  +M+IGDGIN+TVITGN SVVDGWTT+NS+TF + G  F+ +++T RNTAGP K
Sbjct: 314 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 373

Query: 361 HQAVAVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCN 420
            QAVA+R+  DLS FY CSFE YQDTLY HS RQFYRECD+YGTVDFIFGNAA V Q CN
Sbjct: 374 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 433

Query: 421 LYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQY 480
           LY R+P   Q N  TAQGRTDPNQ+TG +IH CTIR A D A  SN T+  YLGRPWK+Y
Sbjct: 434 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEY 493

Query: 481 SRTVYMQSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNAT 540
           SRTV MQ+YI   + P GW  W+G   L TLYY E+ N GPG++T+ RV WPGY ++NAT
Sbjct: 494 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 553

Query: 541 QAMNFTVYNFTMGDTWLPYTDIPFNGGLV 557
            A NFTV NF +G+ W+  T +PF GGL+
Sbjct: 554 DASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150485.10.0e+00100.00probable pectinesterase/pectinesterase inhibitor 25 [Momordica charantia][more]
XP_008446714.11.8e-28787.21PREDICTED: probable pectinesterase/pectinesterase inhibitor 25 [Cucumis melo][more]
XP_004135375.14.1e-28786.68probable pectinesterase/pectinesterase inhibitor 25 [Cucumis sativus] >KGN52152.... [more]
XP_038891461.13.8e-28587.06probable pectinesterase/pectinesterase inhibitor 25 [Benincasa hispida][more]
XP_022976564.11.9e-28485.79probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94CB18.5e-19561.61Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF778.0e-19362.39Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK74.9e-14248.94Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX41.5e-13546.64Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
O222565.8e-13546.75Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1D8L40.0e+00100.00Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1[more]
A0A1S3BFR98.9e-28887.21Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1[more]
A0A0A0KUN82.0e-28786.68Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1[more]
A0A6J1INW09.2e-28585.79Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1[more]
A0A6J1HU461.6e-28186.90Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10720.26.1e-19661.61Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.15.7e-19462.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.13.5e-14348.94Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.11.1e-13646.64Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.14.1e-13646.75Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 21..176
e-value: 1.4E-20
score: 84.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..155
e-value: 2.8E-10
score: 37.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 28..174
e-value: 8.5E-18
score: 65.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 27..180
e-value: 3.9E-24
score: 87.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 28..179
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 242..541
e-value: 1.2E-135
score: 451.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 236..555
e-value: 1.8E-114
score: 384.4
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 6..555
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 6..555
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 28..179
e-value: 6.08053E-32
score: 118.695
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 237..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g37040.1Moc09g37040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity