Moc09g35220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g35220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	mediator of RNA polymerase II transcription subunit 15a-like
Location	chr9: 26957549 .. 26960793 (+)
RNA-Seq Expression	Moc09g35220
Synteny	Moc09g35220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCTGTATGTTCTCCAACAACCGCTCTCTGTTCATCCTCTTCCTTCTTTGTTTTCTTGCCCCTTAAGCATCGCCACTATATAACTCCGATGAAGATCAAGCAACTTTCCACCATTACCGCCTTCAATCGGTTTGGATATGGAAGCAATTCTTGTGGCTGAGCCCTAAAGGTATTCGATCAATCGTTTTTGGTTGCGTTTTTGCCTCATTCTCAACTCTCGCTGATCTTGTTCTTGTTGCGACTGTGAATGTTTCTGTAATCGGCGTCTATTTAGTGCTATGTTTGTGGTTTCTTGTGGCAGTTGAGTTAGATGATGGTTTTTCGTTTTGTTCTTGTGAGTGAACGTGATACTACGGCTTGGAACTCGCTTTAGCGCCTTTTTGTTGCTATTTGTATGATTGTGTTGCATGTTGTACCAGGTGAAAGGATCTTTGGAATCTATTTGGGCGATTATTGGAAATTAGCTTACGGGAAGAGGAGGACGATGGTGCCTGGTTTACCTGAAAATGGAAGAGGTGAGGAACTGAATATCGATATCTTTGTACTTCGCCATCTGCTGCTTTACTTCCTGCAACAGAGTAGAGTAACATAAAATTGGATATCGAGTGCCTCATTTCTTTTGGTTCTAACTTGACGAAGATAATTTTGCAAAAAGATTATTCCCGCGCGCGCGGAAATATGATAGTATATTTGTATATTTTGTCGGGGAGGAAACCTGGATTTAAATCACGTTTTCTTTCATTGAACAAATATGCAGAAAATTTTTGTAGTGAAAATCTTAGGTATCACGAAATTTTGAACAGAAACAATAATCATTCTTTAAACGGCTCAATTAAGTTCCTTCGAGGAGGAAAATTTCAGGAGAAACTTTTTTTAATTTGACGAGTTTCAGAAATGTAATGAACAAACTTTTCTTAGTGGATTGTTCAATGAACAAATTAGTCAAGTGAAGGAATTCCAATTATTGTCTCCCAAGGTAGATCAGTTAATCCTGTTTCCACTGATCTCCAGGAACTTTCATTTCTACTAAAAATTCAGTTGATTTGTCTGCTATTGATTTTGTTAGCTGATTGCGAGGAGGATGCCTGGGATGATTTTTTATTCTCACAAAGAGGAAAGGTGTCTCATCAAATAGTAAATGATGCAGTCCAAGAAGAAAAACCTTTCATCATCTCCACCCCTGGCATGTCTCCTTCAGCCCTTTTGGAAGAAGTATCAGAATCAGATCGTGATAATGACATATGTTCTGTTACTCCTCTTCAACGTTTGCTTAAAATGGTTAGTTAATAAATACTTGTTAGTTTCATATCTCTTTTTAGAACAAGTCGTACGTACATTGTTAAATTTCTGTGCTTGCTCAATCTCAGGTAAACGTAATGTCACCTAAAGCATTAAGTGCTTCTATTAGTGACATAGATTCTATTGTCTGTATGACTGACAGAGTTGCTTCATCAGCGCCCCTAAACAAGTCCAAAGCTGAAATTAGCGAGGATTTGGTGGGCACGGTAACATCTCGTCTGAGAAAAAGATGTTCAATATTTGGAGGCAGTACAGGATCAAGAAGAACAAGACACTGCAGGAATACCAGGATGTATACCAGCTCCAGACGAATGATGTATTGTGAAAGTTATGACTTGAATTCAACATTTACAGAAAGGTCAAGAATCGAGGTGCGTGAGTTATCCGTTTCGTTTATCTATGATTAGAAGCTCGAAAGGGTGAAACTGCCTAGGTGAACAAATAAGAACTTCTGAGAAAATATGATCATTAACCTTGCCAATTCTGTTTGATTGATAAAATAAATTAAATGTGAAGTACTTTTACTCATGAATGTGAAATTAGCTTTCTACCCCAAATGAGGTTAGCAAGATCCATCGATGATAATGTCGATAAGAAGGAATAAGTTGTGTATATAAGAAATTATAAATTATTGAGTTACAAAATCTTGAACACAACTATTTGTTCAACTTCGAATATTTTTTTTTATAAGTTTCCCATTGACAACATAGTTCTTTGATATTTTTTCAAATACTTCTGTTAATATAGACTCCTGAACATTCCCATTGGCGTGGGTTTTCATTGCCGTTCAAGCAATATTTATTAGAAGAAATTAGGAAGATAAATGAGCAGCTGATAGACACAGTGGTGTCTGTATGTGACAAAGATATGATAACAACTGATAATGCAGCTACAAATGACAGAAGTGAAGGGATCATAGTCGAGTGTTCTTTTGCTGCTATGCGCATCAGTTCGAATAAAATCTCACAGCAAGCTTCAGCCCTTCAGGTACTTCAGCCCCATCTATTTCTTAATGTGATTCATCAATTATAGGCTTAACTTTATTTTACTCATTTTATTCATTATGGCATGCAGTTAACAGTCCATCCCTTGAAGTTGCTAGTTCCCAAAGGCTATCCACACTGTTCTCCAATATTCAATAAATTGAAACTGGAAGCTTGGTGAGATTATTTATCCTTTATCCCTTTTTTATTGCACGAATCTGCCTATATATGACCACCTTATGATTCGATCTTTTATATTCTGAGGTCTTTATTTCTTTTTTATTGCTCTAAACAAATGTTCCTAGATGTGCCTTTTTATTTTGGTATAGAGTAGTTAATGAATCCTTATTAACTTCTGAGAATGCCAAAGGGCACAAACACAAGAATTTCCAGTTCCTAAGAACAAGCCTTCTTGAATTATTATGAGTAGATTTATACAGGGAAGCAAAATTAAAACCAGTTGATGGATTTGCATCTATGGTATAGATCCTAGAGATCAAACCTACGGGTTGAGTATCTTTTTTTTTTCCCTTTGTGTAGAGTATGAGTGATAAACATCCTTGGTCCCTCATTGTTTTATCAATCTACGTACATATATAAAAAAGCTCATTAATTTTAATAATGTTTTTCACGAACCATTATGTGTTCCAGGAACAGATGGAGAAAGTAATTCTTAACCATTATTAAATCACCCCCGACAACTTCTCTGTTCCATAGAATTATAACTTGAAAAGCTAACATTTTTGCAGTCCAACAGATTGTGAAGAGCTTTCATTGAAGACAAGGGAAAAGTTCAACATGTGTGTCCGAAGCCTTCCACATCCATTCTCCATAAGTGAAGTGGCGAGAACGTGGGATGCGTGTGCTCGAGAAGTGGTTTCGGAGTTTGCTCAACGAAATGGCGGCGGCAGTTTCACTTCAAAATATGGCTCCTGGGAGGACTGTCTTCACCATGCATAG

mRNA sequence

ATGAGCTCATCGCCACTATATAACTCCGATGAAGATCAAGCAACTTTCCACCATTACCGCCTTCAATCGGTTTGGATATGGAAGCAATTCTTGTGGCTGAGCCCTAAAGGTATTCGATCAATCGTTTTTGGTTGCGTTTTTGCCTCATTCTCAACTCTCGCTGATCTTGTTCTTGTTGCGACTGTGAATGTTTCTGTAATCGGCGTCTATTTAGTGCTATGTTTGTGGTTTCTTGTGGCAGTTGAGTTAGATGATGGTTTTTCGTTTTGTTCTTGTGAAAGGATCTTTGGAATCTATTTGGGCGATTATTGGAAATTAGCTTACGGGAAGAGGAGGACGATGGTGCCTGGTTTACCTGAAAATGGAAGAGCTGATTGCGAGGAGGATGCCTGGGATGATTTTTTATTCTCACAAAGAGGAAAGGTGTCTCATCAAATAGTAAATGATGCAGTCCAAGAAGAAAAACCTTTCATCATCTCCACCCCTGGCATGTCTCCTTCAGCCCTTTTGGAAGAAGTATCAGAATCAGATCGTGATAATGACATATGTTCTGTTACTCCTCTTCAACGTTTGCTTAAAATGGTAAACGTAATGTCACCTAAAGCATTAAGTGCTTCTATTAGTGACATAGATTCTATTGTCTGTATGACTGACAGAGTTGCTTCATCAGCGCCCCTAAACAAGTCCAAAGCTGAAATTAGCGAGGATTTGGTGGGCACGGTAACATCTCGTCTGAGAAAAAGATGTTCAATATTTGGAGGCAGTACAGGATCAAGAAGAACAAGACACTGCAGGAATACCAGGATGTATACCAGCTCCAGACGAATGATGTATTGTGAAAGTTATGACTTGAATTCAACATTTACAGAAAGGTCAAGAATCGAGACTCCTGAACATTCCCATTGGCGTGGGTTTTCATTGCCGTTCAAGCAATATTTATTAGAAGAAATTAGGAAGATAAATGAGCAGCTGATAGACACAGTGGTGTCTGTATGTGACAAAGATATGATAACAACTGATAATGCAGCTACAAATGACAGAAGTGAAGGGATCATAGTCGAGTGTTCTTTTGCTGCTATGCGCATCAGTTCGAATAAAATCTCACAGCAAGCTTCAGCCCTTCAGTTAACAGTCCATCCCTTGAAGTTGCTAGTTCCCAAAGGCTATCCACACTGTTCTCCAATATTCAATAAATTGAAACTGGAAGCTTGTCCAACAGATTGTGAAGAGCTTTCATTGAAGACAAGGGAAAAGTTCAACATGTGTGTCCGAAGCCTTCCACATCCATTCTCCATAAGTGAAGTGGCGAGAACGTGGGATGCGTGTGCTCGAGAAGTGGTTTCGGAGTTTGCTCAACGAAATGGCGGCGGCAGTTTCACTTCAAAATATGGCTCCTGGGAGGACTGTCTTCACCATGCATAG

Coding sequence (CDS)

ATGAGCTCATCGCCACTATATAACTCCGATGAAGATCAAGCAACTTTCCACCATTACCGCCTTCAATCGGTTTGGATATGGAAGCAATTCTTGTGGCTGAGCCCTAAAGGTATTCGATCAATCGTTTTTGGTTGCGTTTTTGCCTCATTCTCAACTCTCGCTGATCTTGTTCTTGTTGCGACTGTGAATGTTTCTGTAATCGGCGTCTATTTAGTGCTATGTTTGTGGTTTCTTGTGGCAGTTGAGTTAGATGATGGTTTTTCGTTTTGTTCTTGTGAAAGGATCTTTGGAATCTATTTGGGCGATTATTGGAAATTAGCTTACGGGAAGAGGAGGACGATGGTGCCTGGTTTACCTGAAAATGGAAGAGCTGATTGCGAGGAGGATGCCTGGGATGATTTTTTATTCTCACAAAGAGGAAAGGTGTCTCATCAAATAGTAAATGATGCAGTCCAAGAAGAAAAACCTTTCATCATCTCCACCCCTGGCATGTCTCCTTCAGCCCTTTTGGAAGAAGTATCAGAATCAGATCGTGATAATGACATATGTTCTGTTACTCCTCTTCAACGTTTGCTTAAAATGGTAAACGTAATGTCACCTAAAGCATTAAGTGCTTCTATTAGTGACATAGATTCTATTGTCTGTATGACTGACAGAGTTGCTTCATCAGCGCCCCTAAACAAGTCCAAAGCTGAAATTAGCGAGGATTTGGTGGGCACGGTAACATCTCGTCTGAGAAAAAGATGTTCAATATTTGGAGGCAGTACAGGATCAAGAAGAACAAGACACTGCAGGAATACCAGGATGTATACCAGCTCCAGACGAATGATGTATTGTGAAAGTTATGACTTGAATTCAACATTTACAGAAAGGTCAAGAATCGAGACTCCTGAACATTCCCATTGGCGTGGGTTTTCATTGCCGTTCAAGCAATATTTATTAGAAGAAATTAGGAAGATAAATGAGCAGCTGATAGACACAGTGGTGTCTGTATGTGACAAAGATATGATAACAACTGATAATGCAGCTACAAATGACAGAAGTGAAGGGATCATAGTCGAGTGTTCTTTTGCTGCTATGCGCATCAGTTCGAATAAAATCTCACAGCAAGCTTCAGCCCTTCAGTTAACAGTCCATCCCTTGAAGTTGCTAGTTCCCAAAGGCTATCCACACTGTTCTCCAATATTCAATAAATTGAAACTGGAAGCTTGTCCAACAGATTGTGAAGAGCTTTCATTGAAGACAAGGGAAAAGTTCAACATGTGTGTCCGAAGCCTTCCACATCCATTCTCCATAAGTGAAGTGGCGAGAACGTGGGATGCGTGTGCTCGAGAAGTGGTTTCGGAGTTTGCTCAACGAAATGGCGGCGGCAGTTTCACTTCAAAATATGGCTCCTGGGAGGACTGTCTTCACCATGCATAG

Protein sequence

MSSSPLYNSDEDQATFHHYRLQSVWIWKQFLWLSPKGIRSIVFGCVFASFSTLADLVLVATVNVSVIGVYLVLCLWFLVAVELDDGFSFCSCERIFGIYLGDYWKLAYGKRRTMVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA

Homology

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BLAST of Moc09g35220 vs. NCBI nr
Match: XP_022138947.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 [Momordica charantia])

HSP 1 Score: 722.6 bits (1864), Expect = 2.2e-204
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0


Query: 114 MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 173
           MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 174 SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 233
           SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 234 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 293
           SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 294 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 353
           IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 354 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL 413
           ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL 300

Query: 414 KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 473
           KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA
Sbjct: 301 KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 360

[Close]

BLAST of Moc09g35220 vs. NCBI nr
Match: XP_022138948.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Momordica charantia])

HSP 1 Score: 571.6 bits (1472), Expect = 6.2e-159
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0


Query: 114 MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 173
           MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 174 SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 233
           SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 234 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 293
           SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 294 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 353
           IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 354 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEA 404
           ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEA
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEA 290

[Close]

BLAST of Moc09g35220 vs. NCBI nr
Match: KAG6601093.1 (Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.3 bits (1375), Expect = 1.1e-147
Identity = 287/472 (60.81%), Postives = 335/472 (70.97%), Query Frame = 0


Query: 3   SSPLYNSDEDQATFHHYRLQSVWIWKQFLWLSPKGIRSIVFGCVFASFSTLADLVLVATV 62
           S PLYN+ ED+AT HH+R QSVW+W+ F  + PKG                         
Sbjct: 20  SIPLYNAHEDEATRHHFRSQSVWLWRLFFSICPKG------------------------- 79

Query: 63  NVSVIGVYLVLCLWFLVAVELDDGFSFCSCERIFGIYLGDYWKLAYGKRRTMVPGLPENG 122
                                         ERIF +YLGD+WKLAY K R M+PGL ENG
Sbjct: 80  ------------------------------ERIFEVYLGDFWKLAYWKWRIMLPGLLENG 139

Query: 123 RADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDI 182
             D E+ A ++FL+         IVNDA Q EK FI+STPGMSPS+LLEEV++SDRDND 
Sbjct: 140 SVDFEK-ALENFLYP-------PIVNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDK 199

Query: 183 CSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVT 242
           C VTPLQRL+KMVNVMSPKALSAS+SDIDSIVCMTDRV+SSAPLNKSK  +  DL GTV 
Sbjct: 200 CCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSSAPLNKSKGAVGMDLAGTVA 259

Query: 243 SRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR-IETPEHSH 302
           SRLR++CS FGG+ GSRRTRH          R +M CE +DL+S FT+RSR IETP+HS+
Sbjct: 260 SRLRRKCSRFGGTIGSRRTRH---------YRCLMDCEGFDLSSLFTQRSRIIETPKHSN 319

Query: 303 WRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMR 362
           W GFSLP KQ LLEEIR+INEQLI T VS+CDKDM+TTD  AT  RSEGI+VECSF A+ 
Sbjct: 320 WHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPGATIGRSEGILVECSFGAVC 379

Query: 363 ISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNM 422
             S+ IS+Q SA+QL +HPLKLLVP GYPH SPIF   K+EACPT CE+LSL+ REKFNM
Sbjct: 380 TLSHLISRQTSAIQLAMHPLKLLVPIGYPHISPIF---KMEACPTGCEDLSLQAREKFNM 416

Query: 423 CVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 474
            VRSL HPFSISEVARTWD CAR+V+SE+A++NGGGSF+SKYGSWEDCLH A
Sbjct: 440 SVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

[Close]

BLAST of Moc09g35220 vs. NCBI nr
Match: XP_023526658.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo] >XP_023526666.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532.7 bits (1371), Expect = 3.2e-147
Identity = 288/472 (61.02%), Postives = 334/472 (70.76%), Query Frame = 0


Query: 3   SSPLYNSDEDQATFHHYRLQSVWIWKQFLWLSPKGIRSIVFGCVFASFSTLADLVLVATV 62
           S  LYN+ ED+AT HH+R QSVW+W+ F  + PKG                         
Sbjct: 20  SISLYNAHEDEATRHHFRSQSVWLWRLFFSICPKG------------------------- 79

Query: 63  NVSVIGVYLVLCLWFLVAVELDDGFSFCSCERIFGIYLGDYWKLAYGKRRTMVPGLPENG 122
                                         ERIF IYLGD+WKLAY K R M+PGL ENG
Sbjct: 80  ------------------------------ERIFEIYLGDFWKLAYRKWRIMLPGLLENG 139

Query: 123 RADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDI 182
             D E+ A ++FL+         IVNDA Q EK FI+STPGMSPS+LLEEV++SDRDND 
Sbjct: 140 SVDFEK-ALENFLYP-------PIVNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDK 199

Query: 183 CSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVT 242
           C VTPLQRL+KMVNVMSPKALSAS+SDIDSIVCMTDRV SSAPLNKSK  +  DL GTV 
Sbjct: 200 CCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVPSSAPLNKSKGAVGMDLAGTVA 259

Query: 243 SRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSRI-ETPEHSH 302
           SRLR++CS FGG+ GSRRTRH          R +M CE +DL+S FT+RSRI ETP+HS+
Sbjct: 260 SRLRRKCSRFGGTNGSRRTRH---------YRCLMDCEGFDLSSLFTQRSRITETPKHSN 319

Query: 303 WRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMR 362
           W GFSLP KQ LLEEIR+INEQLI T VS+CDKDM+TTD+AAT  RSEGI+VECSF A+ 
Sbjct: 320 WHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDSAATIGRSEGILVECSFGAVC 379

Query: 363 ISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNM 422
             S+ IS Q SA+QL +HPLKLLVP GYPH SPIF   K+EACPT CE+LSL+ REKFNM
Sbjct: 380 TLSHLISPQTSAVQLAMHPLKLLVPIGYPHISPIF---KMEACPTGCEDLSLQAREKFNM 416

Query: 423 CVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 474
            VRSL HPFSISEVARTWD CAR+V+SE+A++NGGGSF+SKYGSWEDCLH A
Sbjct: 440 SVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

[Close]

BLAST of Moc09g35220 vs. NCBI nr
Match: XP_022956517.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata] >XP_022956518.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata])

HSP 1 Score: 530.0 bits (1364), Expect = 2.1e-146
Identity = 286/472 (60.59%), Postives = 333/472 (70.55%), Query Frame = 0


Query: 3   SSPLYNSDEDQATFHHYRLQSVWIWKQFLWLSPKGIRSIVFGCVFASFSTLADLVLVATV 62
           S  +YN+ ED+AT HH+R QSVW+W+ F  + PKG                         
Sbjct: 20  SISVYNAHEDEATRHHFRSQSVWLWRLFFSICPKG------------------------- 79

Query: 63  NVSVIGVYLVLCLWFLVAVELDDGFSFCSCERIFGIYLGDYWKLAYGKRRTMVPGLPENG 122
                                         ERIF IYLGD+WKLAY K R M+PGL ENG
Sbjct: 80  ------------------------------ERIFEIYLGDFWKLAYWKWRIMLPGLLENG 139

Query: 123 RADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDI 182
             D E+ A ++FL+         IVNDA Q EK FI+STPGMSPS+LLEEV++SDRDND 
Sbjct: 140 SVDFEK-ALENFLYP-------PIVNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDK 199

Query: 183 CSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVT 242
           C VTPLQRL+KMVNVMSPKALSAS+SDIDSIVCMTDRV+SSAPLNKSK  +  DL GTV 
Sbjct: 200 CCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSSAPLNKSKGAVGMDLAGTVA 259

Query: 243 SRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR-IETPEHSH 302
           SRLR++CS FGG+ GSRRTRH          R +M CE +DL+S FT+RSR IETP+HS+
Sbjct: 260 SRLRRKCSRFGGTIGSRRTRH---------YRCLMDCEGFDLSSLFTQRSRIIETPKHSN 319

Query: 303 WRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMR 362
           W GFSLP KQ LLEEIR+INEQLI T VS+CDKDM+TTD  AT  RSEGI+VECSF A+ 
Sbjct: 320 WHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPGATIGRSEGILVECSFGAVC 379

Query: 363 ISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNM 422
             S+ IS Q SA+QL +HPLKLLVP GYPH SPIF   K+EACPT CE+LSL+ REKFNM
Sbjct: 380 TLSHLISHQTSAIQLAMHPLKLLVPIGYPHISPIF---KMEACPTGCEDLSLQAREKFNM 416

Query: 423 CVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 474
            VRSL HPFSISEVARTWD CAR+V+SE+A++NGGGSF+SKYGSWEDCLH A
Sbjct: 440 SVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

[Close]

BLAST of Moc09g35220 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.1e-44
Identity = 130/356 (36.52%), Postives = 197/356 (55.34%), Query Frame = 0


Query: 140  GKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVT-----------PL 199
            G ++ Q         +   I TPG+S S LL+E +  D  N + S T           P+
Sbjct: 989  GNIARQQATGMQGVVQSLAIGTPGISASPLLQEFTSPD-GNILNSSTITSGKPSATELPI 1048

Query: 200  QRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVTSRLRKR 259
            +RL++ V  +SP+ALS+++SDI S+V M DR+A SAP N S+A + EDLV     RL+ R
Sbjct: 1049 ERLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDLVAMTKCRLQAR 1108

Query: 260  --CSIFGGSTGSRRTRHCRNTRMYTSS---------RRMMYCESYDLNSTFT---ERSRI 319
               +  G     +  RH     +  +S         ++    E+ DL ST T   +++R 
Sbjct: 1109 NFMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKART 1168

Query: 320  ETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMIT--TDNAATNDRSEGII 379
            ET EH+            LLEEI++IN++LIDTVV + D +     ++ A ++   EG  
Sbjct: 1169 ET-EHA------------LLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTT 1228

Query: 380  VECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSP-IFNKLKLEACPTDCEEL 439
            V  SF A+ +S    +  +S     + PL+LLVP  YP+ SP + +KL +E    + E+L
Sbjct: 1229 VRFSFIAVSLSPALKAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN-EDL 1288

Query: 440  SLKTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWE 468
            S K   +FN+ +RSL  P S+ ++A+TWDACAR V+ E+AQ+ GGG+F+SKYG+WE
Sbjct: 1289 SSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWE 1329

[Close]

BLAST of Moc09g35220 vs. ExPASy Swiss-Prot
Match: Q9SHV7 (Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis thaliana OX=3702 GN=MED15C PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.0e-28
Identity = 92/296 (31.08%), Postives = 155/296 (52.36%), Query Frame = 0


Query: 187 PLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLN-KSKAEISEDLVGTVTSRL 246
           P+ RL+K     SPK+L+ S+S+I S++ M D +  S P +  S+A + EDL     S  
Sbjct: 659 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDL-----SER 718

Query: 247 RKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGF 306
            +  +    +  S+R +   N      S ++   +SY+  S+    S + +   S  +  
Sbjct: 719 TRNFTTHEETNLSKRMKRSINIVPPDMSSQI---DSYEQLSSL--ESEVVSTTSSGLKVN 778

Query: 307 SLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRIS-- 366
           ++     LL+EI++ N +L++TVV +CD+D            S G IV C++A + +S  
Sbjct: 779 NIAPGYALLQEIKETNGRLVETVVEICDED------------SLGTIVTCTYAPVALSAT 838

Query: 367 ------SNKISQQAS--ALQLTVHPLKLLVPKGYPHCSPI-FNKLKLEACPTDCEELSLK 426
                 S KI    S   +Q  + PL+LL P  YP+ SPI   ++  +      E+LS +
Sbjct: 839 FKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLSAR 898

Query: 427 TREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCL 471
           TR +F++ ++    P     +A+TW+ CAR  + E+A+R+GGG+F+SKYG+WE  L
Sbjct: 899 TRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVL 932

[Close]

BLAST of Moc09g35220 vs. ExPASy Swiss-Prot
Match: F4I169 (Probable mediator of RNA polymerase II transcription subunit 15b OS=Arabidopsis thaliana OX=3702 GN=MED15B PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.6e-08
Identity = 44/137 (32.12%), Postives = 71/137 (51.82%), Query Frame = 0


Query: 127 EEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFI-----ISTPGMSPSALLEEVSESDRD-- 186
           ++ + DD    Q   +S Q     VQ+ +  +     I TPG+S S LL E++  D +  
Sbjct: 117 QDQSHDDQTTLQMQSMSMQGAGSRVQQIRQGVLQSLEIGTPGISASPLLPELTSPDGNII 176

Query: 187 NDICSV--------TPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKA 246
           N + S          P++RL++ +  +SP+ALS+++ DI S+V M DR+A S P   S+A
Sbjct: 177 NPLTSTCGKSSATELPIERLIRAMKSISPQALSSAVCDIRSVVSMVDRIAGSVPGKGSRA 236

Query: 247 EISEDLVGTVTSRLRKR 249
               DLV      L++R
Sbjct: 237 SFGVDLVAMTKCHLQER 253

[Close]

BLAST of Moc09g35220 vs. ExPASy TrEMBL
Match: A0A6J1CCM7 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010000 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 1.0e-204
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0


Query: 114 MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 173
           MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 174 SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 233
           SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 234 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 293
           SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 294 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 353
           IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 354 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL 413
           ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL 300

Query: 414 KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 473
           KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA
Sbjct: 301 KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 360

[Close]

BLAST of Moc09g35220 vs. ExPASy TrEMBL
Match: A0A6J1CEI3 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010000 PE=4 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 3.0e-159
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0


Query: 114 MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 173
           MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 174 SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 233
           SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 234 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 293
           SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 294 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 353
           IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 354 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEA 404
           ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEA
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEA 290

[Close]

BLAST of Moc09g35220 vs. ExPASy TrEMBL
Match: A0A6J1GWK1 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moschata OX=3662 GN=LOC111458234 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 1.0e-146
Identity = 286/472 (60.59%), Postives = 333/472 (70.55%), Query Frame = 0


Query: 3   SSPLYNSDEDQATFHHYRLQSVWIWKQFLWLSPKGIRSIVFGCVFASFSTLADLVLVATV 62
           S  +YN+ ED+AT HH+R QSVW+W+ F  + PKG                         
Sbjct: 20  SISVYNAHEDEATRHHFRSQSVWLWRLFFSICPKG------------------------- 79

Query: 63  NVSVIGVYLVLCLWFLVAVELDDGFSFCSCERIFGIYLGDYWKLAYGKRRTMVPGLPENG 122
                                         ERIF IYLGD+WKLAY K R M+PGL ENG
Sbjct: 80  ------------------------------ERIFEIYLGDFWKLAYWKWRIMLPGLLENG 139

Query: 123 RADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDI 182
             D E+ A ++FL+         IVNDA Q EK FI+STPGMSPS+LLEEV++SDRDND 
Sbjct: 140 SVDFEK-ALENFLYP-------PIVNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDK 199

Query: 183 CSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVT 242
           C VTPLQRL+KMVNVMSPKALSAS+SDIDSIVCMTDRV+SSAPLNKSK  +  DL GTV 
Sbjct: 200 CCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSSAPLNKSKGAVGMDLAGTVA 259

Query: 243 SRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR-IETPEHSH 302
           SRLR++CS FGG+ GSRRTRH          R +M CE +DL+S FT+RSR IETP+HS+
Sbjct: 260 SRLRRKCSRFGGTIGSRRTRH---------YRCLMDCEGFDLSSLFTQRSRIIETPKHSN 319

Query: 303 WRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMR 362
           W GFSLP KQ LLEEIR+INEQLI T VS+CDKDM+TTD  AT  RSEGI+VECSF A+ 
Sbjct: 320 WHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPGATIGRSEGILVECSFGAVC 379

Query: 363 ISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNM 422
             S+ IS Q SA+QL +HPLKLLVP GYPH SPIF   K+EACPT CE+LSL+ REKFNM
Sbjct: 380 TLSHLISHQTSAIQLAMHPLKLLVPIGYPHISPIF---KMEACPTGCEDLSLQAREKFNM 416

Query: 423 CVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 474
            VRSL HPFSISEVARTWD CAR+V+SE+A++NGGGSF+SKYGSWEDCLH A
Sbjct: 440 SVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

[Close]

BLAST of Moc09g35220 vs. ExPASy TrEMBL
Match: A0A6J1JBE6 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita maxima OX=3661 GN=LOC111482923 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.7e-146
Identity = 286/472 (60.59%), Postives = 333/472 (70.55%), Query Frame = 0


Query: 3   SSPLYNSDEDQATFHHYRLQSVWIWKQFLWLSPKGIRSIVFGCVFASFSTLADLVLVATV 62
           S  LYN+ ED+AT HH+R QSVW+W+ F  + PKG                         
Sbjct: 20  SISLYNAHEDEATRHHFRSQSVWLWRLFFSICPKG------------------------- 79

Query: 63  NVSVIGVYLVLCLWFLVAVELDDGFSFCSCERIFGIYLGDYWKLAYGKRRTMVPGLPENG 122
                                         ERIF IYLGD+WKLAY K R M+PGL ENG
Sbjct: 80  ------------------------------ERIFEIYLGDFWKLAYRKWRIMLPGLLENG 139

Query: 123 RADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDI 182
             D E+ A +DFL+         IVNDA Q EK FI+STPGMSPS+LLEEV++SDRDND 
Sbjct: 140 SVDFEK-ALEDFLYP-------PIVNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDK 199

Query: 183 CSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVT 242
           C VTPLQRL+KMVNVMSPKALSAS+SDIDSIVCMTDRV+SSAPLNKSK  +  DL GTV 
Sbjct: 200 CCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSSAPLNKSKGAVGMDLAGTVA 259

Query: 243 SRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR-IETPEHSH 302
           SRLR++CS FGG+ GSRRTRH          R +M CE +DL+S FT+RSR IETP+HS+
Sbjct: 260 SRLRRKCSRFGGTIGSRRTRH---------YRCLMDCEGFDLSSLFTQRSRIIETPKHSN 319

Query: 303 WRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMR 362
           W GFSLP KQ LLEEIR+INEQLI T VS+CDKDM+TTD AAT  +SEGI+VECSF A+ 
Sbjct: 320 WHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPAATIGKSEGILVECSFGAVC 379

Query: 363 ISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNM 422
             S+ IS Q SA+QL + PLKLLVP GYPH SPIF   K+EACPT CE+LSL+ REKFNM
Sbjct: 380 TLSHLISHQTSAIQLAMQPLKLLVPIGYPHISPIF---KMEACPTGCEDLSLQAREKFNM 416

Query: 423 CVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 474
            VRSL HPFSISE+ARTWD CAR+V+SE+A++NGGGSF+SKYGSWEDCLH A
Sbjct: 440 SVRSLAHPFSISEMARTWDMCARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

[Close]

BLAST of Moc09g35220 vs. ExPASy TrEMBL
Match: A0A5B6ZF98 (KIX_2 domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_011972 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 3.4e-62
Identity = 158/355 (44.51%), Postives = 219/355 (61.69%), Query Frame = 0


Query: 137  SQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVT---------- 196
            S  G + HQ    A+ + +   I TPG+SPS LL E +  D ++   S            
Sbjct: 965  SNAGNIGHQQTAGALAQAQSLAIGTPGISPSPLLAEFTSPDANHANASTVVSGKSSVTEQ 1024

Query: 197  PLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVTSRLR 256
            PL+RL+K+V  MSPKALSAS+SDI S+V M DR+A SAP N S+A + EDL      RL+
Sbjct: 1025 PLERLIKVVKSMSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLFAMTKCRLQ 1084

Query: 257  KRCSIF-GGSTGSRRTRHCRNTRMYTSSRRMMYCESY-DLNSTF-----TERSRIETPEH 316
             R  I   G+TG+R+ R       YTS+  +    S   +N +F     +E S +E+   
Sbjct: 1085 ARNFITQDGNTGTRKMRR------YTSAMPLNVVSSAGSINDSFKQLTGSEASDLESTAT 1144

Query: 317  SHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAA 376
            S  +   +     LLEEIR+IN+QLIDTVV + D+D+  T  AA ++  EG IV+CSF+A
Sbjct: 1145 SSIKRPRIEANHALLEEIREINQQLIDTVVDISDEDIDPT--AAASEGGEGTIVKCSFSA 1204

Query: 377  MRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPI-FNKLKLEACPTDCEELSLKTREK 436
            + +S N  SQ ASA Q++  PL+LLVP  YP+CSPI  +K  +E    + E+LS+K + +
Sbjct: 1205 VALSPNLKSQYASA-QMS-QPLRLLVPTNYPNCSPILLDKFPIEV-SKEYEDLSVKAKSR 1264

Query: 437  FNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 474
            F++ +RSL  P S+ E+ARTWD CAR V+SE+AQ++GGGSF+SKYGSWE CL  A
Sbjct: 1265 FSISLRSLSQPMSLGEIARTWDVCARAVISEYAQQSGGGSFSSKYGSWESCLSAA 1308

[Close]

BLAST of Moc09g35220 vs. TAIR 10
Match: AT1G15780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10440.1); Has 103701 Blast hits to 43153 proteins in 1828 species: Archae - 30; Bacteria - 7385; Metazoa - 38639; Fungi - 11531; Plants - 7727; Viruses - 307; Other Eukaryotes - 38082 (source: NCBI BLink). )

HSP 1 Score: 181.0 bits (458), Expect = 2.2e-45
Identity = 130/356 (36.52%), Postives = 197/356 (55.34%), Query Frame = 0


Query: 140  GKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVT-----------PL 199
            G ++ Q         +   I TPG+S S LL+E +  D  N + S T           P+
Sbjct: 989  GNIARQQATGMQGVVQSLAIGTPGISASPLLQEFTSPD-GNILNSSTITSGKPSATELPI 1048

Query: 200  QRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEISEDLVGTVTSRLRKR 259
            +RL++ V  +SP+ALS+++SDI S+V M DR+A SAP N S+A + EDLV     RL+ R
Sbjct: 1049 ERLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDLVAMTKCRLQAR 1108

Query: 260  --CSIFGGSTGSRRTRHCRNTRMYTSS---------RRMMYCESYDLNSTFT---ERSRI 319
               +  G     +  RH     +  +S         ++    E+ DL ST T   +++R 
Sbjct: 1109 NFMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKART 1168

Query: 320  ETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMIT--TDNAATNDRSEGII 379
            ET EH+            LLEEI++IN++LIDTVV + D +     ++ A ++   EG  
Sbjct: 1169 ET-EHA------------LLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTT 1228

Query: 380  VECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSP-IFNKLKLEACPTDCEEL 439
            V  SF A+ +S    +  +S     + PL+LLVP  YP+ SP + +KL +E    + E+L
Sbjct: 1229 VRFSFIAVSLSPALKAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN-EDL 1288

Query: 440  SLKTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWE 468
            S K   +FN+ +RSL  P S+ ++A+TWDACAR V+ E+AQ+ GGG+F+SKYG+WE
Sbjct: 1289 SSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWE 1329

[Close]

BLAST of Moc09g35220 vs. TAIR 10
Match: AT2G10440.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 1628 Blast hits to 1350 proteins in 149 species: Archae - 0; Bacteria - 39; Metazoa - 480; Fungi - 159; Plants - 187; Viruses - 2; Other Eukaryotes - 761 (source: NCBI BLink). )

HSP 1 Score: 130.2 bits (326), Expect = 4.4e-30
Identity = 88/286 (30.77%), Postives = 151/286 (52.80%), Query Frame = 0


Query: 187 PLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLN-KSKAEISEDLVGTVTSRL 246
           P+ RL+K     SPK+L+ S+S+I S++ M D +  S P +  S+A + EDL     S  
Sbjct: 579 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDL-----SER 638

Query: 247 RKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGF 306
            +  +    +  S+R +   N      S ++   +SY+  S+    S + +   S  +  
Sbjct: 639 TRNFTTHEETNLSKRMKRSINIVPPDMSSQI---DSYEQLSSL--ESEVVSTTSSGLKVN 698

Query: 307 SLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRISSN 366
           ++     LL+EI++ N +L++TVV +CD+D            S G IV C++A + +S+ 
Sbjct: 699 NIAPGYALLQEIKETNGRLVETVVEICDED------------SLGTIVTCTYAPVALSAT 758

Query: 367 KISQQASALQLTVHPLKLLVPKGYPHCSPI-FNKLKLEACPTDCEELSLKTREKFNMCVR 426
                 S     + PL+LL P  YP+ SPI   ++  +      E+LS +TR +F++ ++
Sbjct: 759 FKDHYKSGKIAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLSARTRSRFSLSMK 818

Query: 427 SLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCL 471
               P     +A+TW+ CAR  + E+A+R+GGG+F+SKYG+WE  L
Sbjct: 819 EFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVL 842

[Close]

BLAST of Moc09g35220 vs. TAIR 10
Match: AT2G10440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 8319 Blast hits to 5104 proteins in 317 species: Archae - 0; Bacteria - 285; Metazoa - 1706; Fungi - 535; Plants - 320; Viruses - 18; Other Eukaryotes - 5455 (source: NCBI BLink). )

HSP 1 Score: 127.5 bits (319), Expect = 2.9e-29
Identity = 92/296 (31.08%), Postives = 155/296 (52.36%), Query Frame = 0


Query: 187 PLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLN-KSKAEISEDLVGTVTSRL 246
           P+ RL+K     SPK+L+ S+S+I S++ M D +  S P +  S+A + EDL     S  
Sbjct: 659 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDL-----SER 718

Query: 247 RKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGF 306
            +  +    +  S+R +   N      S ++   +SY+  S+    S + +   S  +  
Sbjct: 719 TRNFTTHEETNLSKRMKRSINIVPPDMSSQI---DSYEQLSSL--ESEVVSTTSSGLKVN 778

Query: 307 SLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRIS-- 366
           ++     LL+EI++ N +L++TVV +CD+D            S G IV C++A + +S  
Sbjct: 779 NIAPGYALLQEIKETNGRLVETVVEICDED------------SLGTIVTCTYAPVALSAT 838

Query: 367 ------SNKISQQAS--ALQLTVHPLKLLVPKGYPHCSPI-FNKLKLEACPTDCEELSLK 426
                 S KI    S   +Q  + PL+LL P  YP+ SPI   ++  +      E+LS +
Sbjct: 839 FKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLSAR 898

Query: 427 TREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCL 471
           TR +F++ ++    P     +A+TW+ CAR  + E+A+R+GGG+F+SKYG+WE  L
Sbjct: 899 TRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVL 932

[Close]

BLAST of Moc09g35220 vs. TAIR 10
Match: AT1G15770.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-09
Identity = 44/137 (32.12%), Postives = 71/137 (51.82%), Query Frame = 0


Query: 127 EEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFI-----ISTPGMSPSALLEEVSESDRD-- 186
           ++ + DD    Q   +S Q     VQ+ +  +     I TPG+S S LL E++  D +  
Sbjct: 117 QDQSHDDQTTLQMQSMSMQGAGSRVQQIRQGVLQSLEIGTPGISASPLLPELTSPDGNII 176

Query: 187 NDICSV--------TPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKA 246
           N + S          P++RL++ +  +SP+ALS+++ DI S+V M DR+A S P   S+A
Sbjct: 177 NPLTSTCGKSSATELPIERLIRAMKSISPQALSSAVCDIRSVVSMVDRIAGSVPGKGSRA 236

Query: 247 EISEDLVGTVTSRLRKR 249
               DLV      L++R
Sbjct: 237 SFGVDLVAMTKCHLQER 253

The following BLAST results are available for this feature:

BLAST of Moc09g35220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138947.1	2.2e-204	100.00	mediator of RNA polymerase II transcription subunit 15a-like isoform X1 [Momordi...	[more]
XP_022138948.1	6.2e-159	100.00	mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Momordi...	[more]
KAG6601093.1	1.1e-147	60.81	Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argy...	[more]
XP_023526658.1	3.2e-147	61.02	mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo sub...	[more]
XP_022956517.1	2.1e-146	60.59	mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata...	[more]

BLAST of Moc09g35220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I171	3.1e-44	36.52	Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana ...	[more]
Q9SHV7	4.0e-28	31.08	Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis ...	[more]
F4I169	1.6e-08	32.12	Probable mediator of RNA polymerase II transcription subunit 15b OS=Arabidopsis ...	[more]

BLAST of Moc09g35220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCM7	1.0e-204	100.00	mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Momor...	[more]
A0A6J1CEI3	3.0e-159	100.00	mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momor...	[more]
A0A6J1GWK1	1.0e-146	60.59	mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moscha...	[more]
A0A6J1JBE6	1.7e-146	60.59	mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita maxima...	[more]
A0A5B6ZF98	3.4e-62	44.51	KIX_2 domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 GN=Di...	[more]

BLAST of Moc09g35220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15780.1	2.2e-45	36.52	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT2G10440.2	4.4e-30	30.77	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT2G10440.1	2.9e-29	31.08	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G15770.1	1.1e-09	32.12	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044661	Mediator of RNA polymerase II transcription subunit 15a/b/c-like	PANTHER	PTHR33137	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED	coord: 140..470
None	No IPR available	PANTHER	PTHR33137:SF27	OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A, PUTATIVE-RELATED	coord: 140..470

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g35220.1	Moc09g35220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
molecular_function	GO:0031490	chromatin DNA binding
molecular_function	GO:0003713	transcription coactivator activity