Moc09g34370 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g34370
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Reverse transcriptase domain-containing protein
Location	chr9: 26288083 .. 26291385 (-)
RNA-Seq Expression	Moc09g34370
Synteny	Moc09g34370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACGAAAGATAGAGGGAGACCGCCCGACCTCACTGGAGAGATGGAGCTCTCTCCCCGTTCCAGATCCGACGAAGTGCGGCAATGATGACGGCGGTGGTCGGTGGCCGGTGGGAAGAAGGTAAGGAGAATAAAGAAGAAGAGGGAGAAATAAAGAGAGATGAAGAATAAAGAAAATGCCCCCTAGGACAACGTTTTACCTCTCATCTAAAAATATAATTTTGAATAATACATTCACCCGTTAATTTGCCACATGCCACATGATTTTTTTATTAAGACAACTCACGAACAAACGATGTTCAGAGACCATTTAGAATATTTTTTTAAATTTTAGGGACAAAAATATATATTTTAAAACTTCATAGACTAAATAGGTACATACTTTAAAACTTTTAACTTAATCTATACTATATTAAGATGGGAGGATATCACAAAAACATTTTTGGACTTTTTTCTCCCTTCCTTAATTTATTAATTTACAAATTATACCATCAATAATTTATTATAAATATTATATTAATTATTAATTATCAATAATAATAAATGTATATGATCTAACCAATGTTAGAATGCAAACGGTTAGAAAACAACACCATAAAATTAAAATTTTTTATTTAATTACCATTCATATTAAGATTAGTGTATAGCTAAAGTTTGATTTCTTTTGTTACTATTTATTGTTTTTTATTGTTCTTTTATAATGTGTTTCATGTATTAAGAATGTTTTTTTAGAAAATAATAATAATGTATTGATCTATTACGAGCTCATGTTGATTTTGTTGGAAAAAAATTTGTGTAAGAGCATGAAAAAGTATTTAATTAATTATAAATAGTAAATTAATAAATTAATTATCAACAATAATTTGTATGAAATCTAAACAGTTTGAGTATATAACTTTTTGTCTATTCATTTTCCAAAAATATAATTTAAAGATCTTTTTTAATAATTTTAATTATAATATAAAGTTAGATTGAAAATACTCCGTACATTTGCACGTGGTATTGAACTAGTAACTAAAAGTGTTCATTTTGAAACTTTAAGATAGGACTAAAAATATATTTTCCCTTGATTTTATTTATTTATATAATATGAGAGGTATGTTGTTTGAAACTTCAAAGAGTCTTGCTCTATTAATATTTATTAATCTATTACAATAAATGTGATGTATTTAATAATATCTTATATTATTATCCAATGGGGTCAAAGTCGTTAGAATGAGATGGTTAATTACATGTTTCTATGGTCAATATTGGTGAACCAAGTGCTAATTAATTACATAACTTATATTATACATTAAAGTTGACGTGATTTTGAAAGGCTTTGAAATGATGCTAGTTTGGTTGTTATAATTAAACCTCACCCAATGTTGTTGAATAGGTAATCTTTCTCTTCATCAATTTCAAACCCATGTTTCCATTTGATTTAGAAACAATAGAATAATATTTCAAAATCAATTATAAGATGTGAACACAAAGAATTGATGCATCGGTTAAAATGATAGTTATTACTATTTTATTTAAAAATTAAAATAATTATTTTAGAAATTAAAAAACCAAAATATAACAAACATAAAAACGATTGAAGTTGCGGTATTTATTTCATTGAAGATTGATCTCTTTACTAGGTGTTATGCTTTATGTTATTTTATTCCTTTTCTTGACGGAGGTCGGCGATCATGGATTTGAATAATAGATATCAAGTACACTTGTATTTATTGATTAATCAATTTCTAAATGCGAGAGGAAGATTCATTGGAAGAGCTGGAAAAAGCTCTGCATTGGCAAAGATCTTGGAGGAATGGGATTCAAGGAAATTAGTGTTTTTAATCAAGCCATGCTAGCCAAGCAATGTTGGAGAATTATAAAAAAATCCAGATAGTTTGGTGACCAGAACTTTGAGGGGCAAATACTTCGTCCATGGCAACTTTATGCGAGCAAAACTGGGGAAAAACCCGTCCTATATGTGGAGAAGTATCCTTTGGGGGAAAAAGCTCTTTGACAAAGGCTTCAGATGGAGAATTGGCAACGGGTCAGCAGTTACAATTGGGATTGATCCCTGGCTCCCAAGGGAAGGAAGTAGTAGGATCCTTCAAGCCCAAGAGTCCTTAAAATACAACAGTGTGTCTCTTTTGATTAGGGAGAGAGGCAGCTGGAATGAAGAGCTCATCAGGGAGTCCTTCAATGCTCAAGAGGCCGAAGCAATTCTCCAAATCCCTCTTCCTAGACATTGTCGGGCAGATGAAGTCATTTGGAATAGAGATAAAAGGGGAGTGTTCAGTGTGAAAAGTGCTTATAAATTGGGAATGGAACTTGAGAACGCTAATTTAGCTTCCACTTCCAATTTGGATTCCCATCGACAAATGTGGAAGAAATTCTGGAAGTCAAAAGCTGCCCCTAAAGTTAAAATTTGCTGTTGGAAAATTTACCACGATATCATTCCAACCAAAATGAACCTTAAGTAGAAAGGAATTTCAACTGATCTTGTTTGTGGCTTGTGTAGGAGATGGTTTCTCCTTTTATGGGAATGTAAGGTAACTAGGAATATTTGGAGTAAATTTCTTCCTACTTACTATGCTCATTTTGGTGTTAACAGAGCTCATTGGACGAACAAGGACAGTTGGAACTAGTTGGTGAACATGCTTTCAGATGAAGAGGTGGCTACTAGCATGGTTATTGCTTGGCAGATATGGGATAGCAGAAACAGAAGCATCTTTAGAGGAGAAACCATTGATGAACAACAACTAGGCAGATCAATAGTCTTGTTCATAAATTCCAACATAGATAAAGGCACATGCATATCCCAAACGAGAAGGAGCCAACAAAATGATGGGTACCTGCCACGAGGAAGGGAGAATCTCAACATGCTTGGCGCTCGATGGAGTGCTCCCCGTACCAATTGCTGGAAACTCAATACAGATGCATCGTGGAGTGAGGAGCGCGAAGTAGGTGGCCTAGGGTGGATTCTTCGTGACTGTAGGGGGGAGATTGTTTTGGCTGGAAACTGTAAAATCAGGGAGAAGAAGGAAATCAAAGCCCTAGAACTAATGGCGATAATTCGAGGACTCCAATCCATCAACATGCAAAGTAGAAGTCCAATTTACCTTGAATTAGATTCAGTCGAAGTAACTCGATTAATGAAGAAGGAGGACGTCGATCTAACAGAAAATATTTGGCTTGTTGAGGAAGCTGTGGAATTAGCGAAAACGAGAGGAATTGTTTCGATCGCCCACATCAACAGAGGGAATAATTCAGTGGCCCACGAATTGGCGAGGAGAGCGACGCACAACGATGAGGAAGAATGA

mRNA sequence

ATGGAACGAAAGATAGAGGGAGACCGCCCGACCTCACTGGAGAGATGGAGCTCTCTCCCCGTTCCAGATCCGACGAAGTGCGGCAATGATGACGGCGGTGGTCGGTGGCCGGTGGGAAGAAGGGAGAGAGGCAGCTGGAATGAAGAGCTCATCAGGGAGTCCTTCAATGCTCAAGAGGCCGAAGCAATTCTCCAAATCCCTCTTCCTAGACATTGTCGGGCAGATGAAGTCATTTGGAATAGAGATAAAAGGGGAGTGTTCAGTGTGAAAAGTGCTTATAAATTGGGAATGGAACTTGAGAACGCTAATTTAGCTTCCACTTCCAATTTGGATTCCCATCGACAAATGAGATGGTTTCTCCTTTTATGGGAATATGAAGAGGTGGCTACTAGCATGGTTATTGCTTGGCAGATATGGGATAGCAGAAACAGAAGCATCTTTAGAGGAGAAACCATTGATGAACAACAACTAGGCAGATCAATAGTCTTGTTCATAAATTCCAACATAGATAAAGGCACATGCATATCCCAAACGAGAAGGAGCCAACAAAATGATGGGTACCTGCCACGAGGAAGGGAGAATCTCAACATGCTTGGCGCTCGATGGAGTGCTCCCCGTACCAATTGCTGGAAACTCAATACAGATGCATCGTGGAGTGAGGAGCGCGAAGTAGGTGGCCTAGGGTGGATTCTTCGTGACTGTAGGGGGGAGATTGTTTTGGCTGGAAACTGTAAAATCAGGGAGAAGAAGGAAATCAAAGCCCTAGAACTAATGGCGATAATTCGAGGACTCCAATCCATCAACATGCAAAGTAGAAGTCCAATTTACCTTGAATTAGATTCAGTCGAAGTAACTCGATTAATGAAGAAGGAGGACGTCGATCTAACAGAAAATATTTGGCTTGTTGAGGAAGCTGTGGAATTAGCGAAAACGAGAGGAATTGTTTCGATCGCCCACATCAACAGAGGGAATAATTCAGTGGCCCACGAATTGGCGAGGAGAGCGACGCACAACGATGAGGAAGAATGA

Coding sequence (CDS)

ATGGAACGAAAGATAGAGGGAGACCGCCCGACCTCACTGGAGAGATGGAGCTCTCTCCCCGTTCCAGATCCGACGAAGTGCGGCAATGATGACGGCGGTGGTCGGTGGCCGGTGGGAAGAAGGGAGAGAGGCAGCTGGAATGAAGAGCTCATCAGGGAGTCCTTCAATGCTCAAGAGGCCGAAGCAATTCTCCAAATCCCTCTTCCTAGACATTGTCGGGCAGATGAAGTCATTTGGAATAGAGATAAAAGGGGAGTGTTCAGTGTGAAAAGTGCTTATAAATTGGGAATGGAACTTGAGAACGCTAATTTAGCTTCCACTTCCAATTTGGATTCCCATCGACAAATGAGATGGTTTCTCCTTTTATGGGAATATGAAGAGGTGGCTACTAGCATGGTTATTGCTTGGCAGATATGGGATAGCAGAAACAGAAGCATCTTTAGAGGAGAAACCATTGATGAACAACAACTAGGCAGATCAATAGTCTTGTTCATAAATTCCAACATAGATAAAGGCACATGCATATCCCAAACGAGAAGGAGCCAACAAAATGATGGGTACCTGCCACGAGGAAGGGAGAATCTCAACATGCTTGGCGCTCGATGGAGTGCTCCCCGTACCAATTGCTGGAAACTCAATACAGATGCATCGTGGAGTGAGGAGCGCGAAGTAGGTGGCCTAGGGTGGATTCTTCGTGACTGTAGGGGGGAGATTGTTTTGGCTGGAAACTGTAAAATCAGGGAGAAGAAGGAAATCAAAGCCCTAGAACTAATGGCGATAATTCGAGGACTCCAATCCATCAACATGCAAAGTAGAAGTCCAATTTACCTTGAATTAGATTCAGTCGAAGTAACTCGATTAATGAAGAAGGAGGACGTCGATCTAACAGAAAATATTTGGCTTGTTGAGGAAGCTGTGGAATTAGCGAAAACGAGAGGAATTGTTTCGATCGCCCACATCAACAGAGGGAATAATTCAGTGGCCCACGAATTGGCGAGGAGAGCGACGCACAACGATGAGGAAGAATGA

Protein sequence

MERKIEGDRPTSLERWSSLPVPDPTKCGNDDGGGRWPVGRRERGSWNEELIRESFNAQEAEAILQIPLPRHCRADEVIWNRDKRGVFSVKSAYKLGMELENANLASTSNLDSHRQMRWFLLLWEYEEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIKALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLTENIWLVEEAVELAKTRGIVSIAHINRGNNSVAHELARRATHNDEEE

Homology

[Close]

BLAST of Moc09g34370 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 326.2 bits (835), Expect = 3.3e-85
Identity = 161/179 (89.94%), Postives = 165/179 (92.18%), Query Frame = 0


Query: 118 WFLLLWEYEEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQ 177
           W + +   EEVATSMVIAWQIW+SRNRSIFRGETIDEQQL RSIVLFINSNIDKGTCISQ
Sbjct: 21  WLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLDRSIVLFINSNIDKGTCISQ 80

Query: 178 TRRSQQNDGYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGE 237
           TRRSQQNDGYLPRGRENLNMLG RWSAP TNCWKLNTDASWSEEREVGG+GWIL DCRGE
Sbjct: 81  TRRSQQNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGE 140

Query: 238 IVLAGNCKIREKKEIKALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLT 297
           IVLAGNCKIREKKEI ALELM IIRGLQ INMQSRSPIYLE DSVEV RLMKKEDVDLT
Sbjct: 141 IVLAGNCKIREKKEINALELMTIIRGLQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of Moc09g34370 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 166.8 bits (421), Expect = 3.3e-37
Identity = 93/217 (42.86%), Postives = 134/217 (61.75%), Query Frame = 0


Query: 126 EEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQND 185
           EE   SM+IAWQIW+ RN+SIF+G   + + +  +I  +I ++  + T +    +S   D
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLK--GKSTNKD 66

Query: 186 GYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCK 245
            +L R  E  +  GA+W  P +N WKLNT+A+W  +   GG+GWILRD +GE++ A    
Sbjct: 67  LHLIRRIE--DNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRI 126

Query: 246 IREKKEIKALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLTENIWLVEEA 305
           IR ++ I  LE+MAI  GL++I  +   PI+LE DS+E   L+ ++  D TE IWL+EE 
Sbjct: 127 IRAERNITYLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEI 186

Query: 306 VELAKTRGIVSIAHINRGNNSVAHELARRATHNDEEE 343
            ++ K   IVS+ HI+R  N VAH LARRA  ND  E
Sbjct: 187 WQMMKDMEIVSMRHISREANKVAHGLARRAMENDLRE 219

[Close]

BLAST of Moc09g34370 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 159.1 bits (401), Expect = 6.9e-35
Identity = 95/225 (42.22%), Postives = 134/225 (59.56%), Query Frame = 0


Query: 126 EEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQND 185
           EE   SM+IAWQIW+ RN+SIF+G   + + +   I  +I ++  + T +    +S   D
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLK--GKSANKD 66

Query: 186 GYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCK 245
            +L R R   N  GARW  P +N WKLNTDA+W  +   GG+GWILRD +GE++ A    
Sbjct: 67  LHLIR-RIGDN-TGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRI 126

Query: 246 IREKKEIKALELMAIIRGLQS--------INMQSRSPIYLELDSVEVTRLMKKEDVDLTE 305
           IR ++ I  LE+MAI  GL++        I  +   PI+LE DS+E   L+ ++  D TE
Sbjct: 127 IRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTE 186

Query: 306 NIWLVEEAVELAKTRGIVSIAHINRGNNSVAHELARRATHNDEEE 343
            IWL+EE  ++ +   IVS+ HI+R  N VAH+LARRA  ND  E
Sbjct: 187 IIWLLEEIWQMMEDMKIVSMRHISREANKVAHDLARRAMENDLRE 227

[Close]

BLAST of Moc09g34370 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 129.8 bits (325), Expect = 4.5e-26
Identity = 74/212 (34.91%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 126 EEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQND 185
           E++   ++ +W IW+ RN  IFRGE      + + +  F+            T  S Q++
Sbjct: 8   EDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFV------------TESSYQSE 67

Query: 186 GYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCK 245
             L    + LN    +W  P  + W LN DASWS+    GG+GWI+R   G+IVLAGN  
Sbjct: 68  TSLSMLHKTLNN-KLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRF 127

Query: 246 IREKKEIKALELMAIIRGLQSI-NMQSRSPIYLELDSVEVTRLMKKEDVDLTENIWLVEE 305
           +     +K LE  AI+ GL+++ N+    P+++E DS EV  L+ ++  DLT+  W+VEE
Sbjct: 128 VEACNNVKLLEASAILEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEE 187

Query: 306 AVELAKTRGIVSIAHINRGNNSVAHELARRAT 337
            + L  +  I++ A + R  N  AH LA+RA+
Sbjct: 188 ILNLRDSCEILAFAKVGRETNGCAHSLAQRAS 206

[Close]

BLAST of Moc09g34370 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 116.3 bits (290), Expect = 5.1e-22
Identity = 72/207 (34.78%), Postives = 109/207 (52.66%), Query Frame = 0


Query: 87  FSVKSAYKLGMELENAN---LASTSNLDSHRQMRWFLLLWEYEEVATSMVIAWQIWDSRN 146
           F  KS   L     N N   L   +N  +     W +     EE   SM+IA QIW+ RN
Sbjct: 108 FFDKSMVMLEEPRANCNFNELEFRTNWTTKEYWEWLMDKAGEEERRRSMIIACQIWEMRN 167

Query: 147 RSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGARWS 206
           +SIF+G   + + +  +I  +I ++  + T     +R  ++   + R  +N     ARW 
Sbjct: 168 KSIFKGVHSETRDIQLAIDRYIINSAGQDT---NLKRKSKDFHPIRRIGDNTR---ARWK 227

Query: 207 APRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIKALELMAIIRG 266
            P +N WKLNTDA+W  +    G+GWILRD +GE++  G   IR ++ I  LE+MAI  G
Sbjct: 228 PPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEG 287

Query: 267 LQSINMQSRSPIYLELDSVEVTRLMKK 291
           L++I  +   PI+LE DS+E   L+ +
Sbjct: 288 LRAIRQEHCRPIHLESDSLEAIHLLHR 308

[Close]

BLAST of Moc09g34370 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.6e-85
Identity = 161/179 (89.94%), Postives = 165/179 (92.18%), Query Frame = 0


Query: 118 WFLLLWEYEEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQ 177
           W + +   EEVATSMVIAWQIW+SRNRSIFRGETIDEQQL RSIVLFINSNIDKGTCISQ
Sbjct: 21  WLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLDRSIVLFINSNIDKGTCISQ 80

Query: 178 TRRSQQNDGYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGE 237
           TRRSQQNDGYLPRGRENLNMLG RWSAP TNCWKLNTDASWSEEREVGG+GWIL DCRGE
Sbjct: 81  TRRSQQNDGYLPRGRENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGE 140

Query: 238 IVLAGNCKIREKKEIKALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLT 297
           IVLAGNCKIREKKEI ALELM IIRGLQ INMQSRSPIYLE DSVEV RLMKKEDVDLT
Sbjct: 141 IVLAGNCKIREKKEINALELMTIIRGLQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of Moc09g34370 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.6e-37
Identity = 93/217 (42.86%), Postives = 134/217 (61.75%), Query Frame = 0


Query: 126 EEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQND 185
           EE   SM+IAWQIW+ RN+SIF+G   + + +  +I  +I ++  + T +    +S   D
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLK--GKSTNKD 66

Query: 186 GYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCK 245
            +L R  E  +  GA+W  P +N WKLNT+A+W  +   GG+GWILRD +GE++ A    
Sbjct: 67  LHLIRRIE--DNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRI 126

Query: 246 IREKKEIKALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLTENIWLVEEA 305
           IR ++ I  LE+MAI  GL++I  +   PI+LE DS+E   L+ ++  D TE IWL+EE 
Sbjct: 127 IRAERNITYLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEI 186

Query: 306 VELAKTRGIVSIAHINRGNNSVAHELARRATHNDEEE 343
            ++ K   IVS+ HI+R  N VAH LARRA  ND  E
Sbjct: 187 WQMMKDMEIVSMRHISREANKVAHGLARRAMENDLRE 219

[Close]

BLAST of Moc09g34370 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 3.3e-35
Identity = 95/225 (42.22%), Postives = 134/225 (59.56%), Query Frame = 0


Query: 126 EEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQND 185
           EE   SM+IAWQIW+ RN+SIF+G   + + +   I  +I ++  + T +    +S   D
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLK--GKSANKD 66

Query: 186 GYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCK 245
            +L R R   N  GARW  P +N WKLNTDA+W  +   GG+GWILRD +GE++ A    
Sbjct: 67  LHLIR-RIGDN-TGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRI 126

Query: 246 IREKKEIKALELMAIIRGLQS--------INMQSRSPIYLELDSVEVTRLMKKEDVDLTE 305
           IR ++ I  LE+MAI  GL++        I  +   PI+LE DS+E   L+ ++  D TE
Sbjct: 127 IRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTE 186

Query: 306 NIWLVEEAVELAKTRGIVSIAHINRGNNSVAHELARRATHNDEEE 343
            IWL+EE  ++ +   IVS+ HI+R  N VAH+LARRA  ND  E
Sbjct: 187 IIWLLEEIWQMMEDMKIVSMRHISREANKVAHDLARRAMENDLRE 227

[Close]

BLAST of Moc09g34370 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.2e-26
Identity = 74/212 (34.91%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 126 EEVATSMVIAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQND 185
           E++   ++ +W IW+ RN  IFRGE      + + +  F+            T  S Q++
Sbjct: 8   EDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFV------------TESSYQSE 67

Query: 186 GYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCK 245
             L    + LN    +W  P  + W LN DASWS+    GG+GWI+R   G+IVLAGN  
Sbjct: 68  TSLSMLHKTLNN-KLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRF 127

Query: 246 IREKKEIKALELMAIIRGLQSI-NMQSRSPIYLELDSVEVTRLMKKEDVDLTENIWLVEE 305
           +     +K LE  AI+ GL+++ N+    P+++E DS EV  L+ ++  DLT+  W+VEE
Sbjct: 128 VEACNNVKLLEASAILEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEE 187

Query: 306 AVELAKTRGIVSIAHINRGNNSVAHELARRAT 337
            + L  +  I++ A + R  N  AH LA+RA+
Sbjct: 188 ILNLRDSCEILAFAKVGRETNGCAHSLAQRAS 206

[Close]

BLAST of Moc09g34370 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.5e-22
Identity = 72/207 (34.78%), Postives = 109/207 (52.66%), Query Frame = 0


Query: 87  FSVKSAYKLGMELENAN---LASTSNLDSHRQMRWFLLLWEYEEVATSMVIAWQIWDSRN 146
           F  KS   L     N N   L   +N  +     W +     EE   SM+IA QIW+ RN
Sbjct: 108 FFDKSMVMLEEPRANCNFNELEFRTNWTTKEYWEWLMDKAGEEERRRSMIIACQIWEMRN 167

Query: 147 RSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRENLNMLGARWS 206
           +SIF+G   + + +  +I  +I ++  + T     +R  ++   + R  +N     ARW 
Sbjct: 168 KSIFKGVHSETRDIQLAIDRYIINSAGQDT---NLKRKSKDFHPIRRIGDNTR---ARWK 227

Query: 207 APRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIKALELMAIIRG 266
            P +N WKLNTDA+W  +    G+GWILRD +GE++  G   IR ++ I  LE+MAI  G
Sbjct: 228 PPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEG 287

Query: 267 LQSINMQSRSPIYLELDSVEVTRLMKK 291
           L++I  +   PI+LE DS+E   L+ +
Sbjct: 288 LRAIRQEHCRPIHLESDSLEAIHLLHR 308

[Close]

BLAST of Moc09g34370 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 6.7e-12
Identity = 54/202 (26.73%), Postives = 93/202 (46.04%), Query Frame = 0


Query: 134 IAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRE 193
           + W++W SRN  +F+G+  D  ++ R  +     + ++ +    TRR  +     P+   
Sbjct: 80  LLWRLWKSRNELMFKGKEYDAPEVLRRAM----EDFEEWS----TRRELEGKASGPQVER 139

Query: 194 NLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIK 253
           NL++   +W AP     K NTDA+W  E    G+GWILR+  G ++  G   +   K + 
Sbjct: 140 NLSV---QWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVL 199

Query: 254 ALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLTENIWLVEEAVELAKTRG 313
             EL A+   + +++  +   I  E D+  +  L+  +D   T    L E+  +L     
Sbjct: 200 EAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWPTLQPAL-EDIQQLLHHFE 259

Query: 314 IVSIAHINRGNNSVAHELARRA 336
            V      RG N VA  +AR +
Sbjct: 260 EVKFEFTPRGGNKVADRIARES 269

[Close]

BLAST of Moc09g34370 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.3e-11
Identity = 53/216 (24.54%), Postives = 101/216 (46.76%), Query Frame = 0


Query: 127 EVATSMV--IAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQN 186
           E A+ +V  + W++W +RN  +FRG   + Q++ R        ++++     + R   ++
Sbjct: 354 EKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRR----AEDDLEEW----RIRTEAES 413

Query: 187 DGYLPRGRENLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNC 246
            G  P  + N +  G RW  P     K NTDA+W+ + E  G+GW+LR+ +GE+   G  
Sbjct: 414 CGTKP--QVNRSSCG-RWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGAR 473

Query: 247 KIREKKEIKALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLMKKEDVDLTENIW---- 306
            + + K +   EL A+   + S++    + +  E DS  +  ++  ++      IW    
Sbjct: 474 ALPKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNNDE------IWPSLK 533

Query: 307 -LVEEAVELAKTRGIVSIAHINRGNNSVAHELARRA 336
             +++   L      V    I R  N++A  +AR +
Sbjct: 534 PTIQDLQRLLSQFTEVKFVFIPREGNTLAERVARES 552

[Close]

BLAST of Moc09g34370 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-08
Identity = 40/155 (25.81%), Postives = 68/155 (43.87%), Query Frame = 0


Query: 134 IAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRE 193
           + W+IW S N  +F       Q    ++ + +N   D    +  T  ++Q +G     R 
Sbjct: 47  LMWRIWKSGNDLVFNHTRTKFQ---TTVEMALN---DTKEWLDNTMTNEQQNG----NRN 106

Query: 194 NLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIK 253
                  +WS P  +  K N DAS  E   V GLGWILR+ +G ++  G  K + +   +
Sbjct: 107 ADPSRNTKWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTE 166

Query: 254 ALELMAIIRGLQSINMQSRSPIYLELDSVEVTRLM 289
             E   +I  +Q+        +  E D+  +TR++
Sbjct: 167 EAECSTLIWAIQASYGFGHKKVIFEGDNQTITRMI 191

[Close]

BLAST of Moc09g34370 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-07
Identity = 48/207 (23.19%), Postives = 79/207 (38.16%), Query Frame = 0


Query: 134 IAWQIWDSRNRSIFRGETIDEQQLGRSIVLFINSNIDKGTCISQTRRSQQNDGYLPRGRE 193
           I W++W SRN  +F+ +        R  +      ++       T      +      R+
Sbjct: 143 IMWRLWKSRNVFLFQQKCQSPDYEARKGIQDATEWLNANETTENTNVHVATNPIQTSRRD 202

Query: 194 NLNMLGARWSAPRTNCWKLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIK 253
           +     ++W+ P     K N D+ +++       GW +R+C G IVL GN K++      
Sbjct: 203 S-----SQWNPPPEGWVKCNFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSL 262

Query: 254 ALELMAIIRGLQSINMQSRSPIYLELDSVE-VTRLMKKEDVDLTENIWLVEEAVELAKTR 313
             E +  +  LQ I       ++ E DS   VT +   ED  L     L+ +        
Sbjct: 263 HAEALGFLHALQVIWAHGLRYVWFESDSKSLVTLINNGEDHSLLGT--LIYDIRHWMLKL 322

Query: 314 GIVSIAHINRGNNSVAHELARRATHND 340
              S+  +NR  NS A  LA      D
Sbjct: 323 PYCSLEFVNRERNSAADALASHVHARD 342

[Close]

BLAST of Moc09g34370 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 43.1 bits (100), Expect = 5.2e-04
Identity = 24/78 (30.77%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 211 KLNTDASWSEEREVGGLGWILRDCRGEIVLAGNCKIREKKEIKALELMAIIRGLQSINMQ 270
           K N DAS  E   V GLGW++R+ +G ++  G  K + +   +  E  A+I  +Q+ +  
Sbjct: 49  KCNYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKFQGRMTPEEAECSALIWAIQATSAF 108

Query: 271 SRSPIYLELDSVEVTRLM 289
             + +  E D+  V RL+
Sbjct: 109 GYTKVIFEGDNSNVNRLI 126

The following BLAST results are available for this feature:

BLAST of Moc09g34370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143317.1	3.3e-85	89.94	uncharacterized protein LOC111013216 [Momordica charantia]	[more]
XP_022143535.1	3.3e-37	42.86	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	6.9e-35	42.22	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022155262.1	4.5e-26	34.91	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022154991.1	5.1e-22	34.78	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]

BLAST of Moc09g34370 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc09g34370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQG0	1.6e-85	89.94	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1CP26	1.6e-37	42.86	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	3.3e-35	42.22	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DNV9	2.2e-26	34.91	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DQC9	2.5e-22	34.78	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Moc09g34370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	6.7e-12	26.73	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	3.3e-11	24.54	Ribonuclease H-like superfamily protein	[more]
AT5G65005.1	2.0e-08	25.81	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G02650.1	2.9e-07	23.19	Ribonuclease H-like superfamily protein	[more]
AT1G52990.1	5.2e-04	30.77	thioredoxin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 213..335 e-value: 2.1E-21 score: 76.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 210..341 e-value: 3.1E-15 score: 58.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..41
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 197..338
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 212..333 e-value: 1.66946E-19 score: 81.2064
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 208..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g34370.1	Moc09g34370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity