Moc09g32900 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g32900
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionProtein-serine/threonine phosphatase
Locationchr9: 24889223 .. 24890599 (-)
RNA-Seq ExpressionMoc09g32900
SyntenyMoc09g32900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTCACTGCTATACAATAAGTCATCGCTCAACTGTTCTCGTTCTTCTTCTTCTCCTAGATCGGTGTCGAGCCCGAGGAAGATTCATGTTTCTCCGTCGTCTCCGCCGCCGCCGTGTCAGCCATCTGTGTTGCCGTTGGCTGTTGATACGGTTCTGAAGAGGAAGAGGCCGGCAAGGATTGCCGTGCCGGTGATTTCGTCCGCGGCCGTTAGTTTTGGATTTGGATTTGAGTCGGAGAAAGTCGAGGCGATGGAGGAGGAAGGGGAGGGATATTCGGTTTATTGCAAGAGAGGAAGGAGACGCAGCGCTATGGAGGATCGATTCTCGGCCAGCGTTGGAATTCGTGGAGATTCTCGACAGGTACTTGGATATTAGATCTCAATCTGCGCGCGGCTTCGTTCCGTTTGATTTCAGAATTAACGCAACTCGTAAGATTAAATCATATCTGTAGATCTGTAGTTTCTTATTGTTCATTTTTTTTTCCGTAACAGGCTTTCTTCGGAGTTTTCGACGGGCACGGCGGAGCAAAAGTGGCAGAAATCGCCGCGAAAAGAATGAGCAAGAACGTGATCGATGAAGTAGCGAGAAGAACAGAATCGGAACTCGAGGAAGCAATCAAGGACGGCTATTCGACGACCGATCGAGAAGTATCAGAGGAAGGCGTCAGCGGCGGCGCCTGCTGCGTCGCCGCATTGATCCGGAACGGAAATCTCGCAGTCTCCAACGTAGGCGATTGTCGCGCCGTGTTGAGCAGAAGGGGAAAAGCAGAAGCCCTAACGTCCGATCACCGCCCGTCCAGAGAAGACGAGAGAAATCGGATCGAAAATTCGGTAAGGCCAGCACCTGTTCATCGATAATCGAATCAATTTGGCGATTGATTTGAAGATCATATTGTTCTTAATTTGAATATATGTCGTTCACAGGGTGGTTATGTAGATTGTTGCCGTGGAGCGTGGAGAGTTCAAGGAACACTAGCAGTGTCGCGAGCGATCGGAGATGGACATTTGAAACAGTGGGTGATATCGGAGCCGGAAACGAGAGTGGTGAAGATTGAGGATGATTGCGATTTCTTGATTCTTGCTTCTGATGGTCTTTGGGACAAGGTAACCAATTAATTAACTAAGCCATTACTCGTACAGCTCTCAACACAAAATATCTACACAAATTTTAAGATTATGAAAAAAACTGAAATTAATTTCTTCAATTTTCCAGGTTACGAATCAGGAAGCGGTAGATATAGTGCAGCCGCTGTGCGTAGGGGCGGAGAAGCCGAAACTATTCTCGGCGTGTAAAGAACTCGCCGCCTTGTCCACATCGAGAGGCTCAATCGACGATACGAGCGTCATGATCATCAAATTACCATCCTTTATCTGA

mRNA sequence

ATGGTTTCACTGCTATACAATAAGTCATCGCTCAACTGTTCTCGTTCTTCTTCTTCTCCTAGATCGGTGTCGAGCCCGAGGAAGATTCATGTTTCTCCGTCGTCTCCGCCGCCGCCGTGTCAGCCATCTGTGTTGCCGTTGGCTGTTGATACGGTTCTGAAGAGGAAGAGGCCGGCAAGGATTGCCGTGCCGGTGATTTCGTCCGCGGCCGTTAGTTTTGGATTTGGATTTGAGTCGGAGAAAGTCGAGGCGATGGAGGAGGAAGGGGAGGGATATTCGGTTTATTGCAAGAGAGGAAGGAGACGCAGCGCTATGGAGGATCGATTCTCGGCCAGCGTTGGAATTCGTGGAGATTCTCGACAGGCTTTCTTCGGAGTTTTCGACGGGCACGGCGGAGCAAAAGTGGCAGAAATCGCCGCGAAAAGAATGAGCAAGAACGTGATCGATGAAGTAGCGAGAAGAACAGAATCGGAACTCGAGGAAGCAATCAAGGACGGCTATTCGACGACCGATCGAGAAGTATCAGAGGAAGGCGTCAGCGGCGGCGCCTGCTGCGTCGCCGCATTGATCCGGAACGGAAATCTCGCAGTCTCCAACGTAGGCGATTGTCGCGCCGTGTTGAGCAGAAGGGGAAAAGCAGAAGCCCTAACGTCCGATCACCGCCCGTCCAGAGAAGACGAGAGAAATCGGATCGAAAATTCGGGTGGTTATGTAGATTGTTGCCGTGGAGCGTGGAGAGTTCAAGGAACACTAGCAGTGTCGCGAGCGATCGGAGATGGACATTTGAAACAGTGGGTGATATCGGAGCCGGAAACGAGAGTGGTGAAGATTGAGGATGATTGCGATTTCTTGATTCTTGCTTCTGATGGTCTTTGGGACAAGGTTACGAATCAGGAAGCGGTAGATATAGTGCAGCCGCTGTGCGTAGGGGCGGAGAAGCCGAAACTATTCTCGGCGTGTAAAGAACTCGCCGCCTTGTCCACATCGAGAGGCTCAATCGACGATACGAGCGTCATGATCATCAAATTACCATCCTTTATCTGA

Coding sequence (CDS)

ATGGTTTCACTGCTATACAATAAGTCATCGCTCAACTGTTCTCGTTCTTCTTCTTCTCCTAGATCGGTGTCGAGCCCGAGGAAGATTCATGTTTCTCCGTCGTCTCCGCCGCCGCCGTGTCAGCCATCTGTGTTGCCGTTGGCTGTTGATACGGTTCTGAAGAGGAAGAGGCCGGCAAGGATTGCCGTGCCGGTGATTTCGTCCGCGGCCGTTAGTTTTGGATTTGGATTTGAGTCGGAGAAAGTCGAGGCGATGGAGGAGGAAGGGGAGGGATATTCGGTTTATTGCAAGAGAGGAAGGAGACGCAGCGCTATGGAGGATCGATTCTCGGCCAGCGTTGGAATTCGTGGAGATTCTCGACAGGCTTTCTTCGGAGTTTTCGACGGGCACGGCGGAGCAAAAGTGGCAGAAATCGCCGCGAAAAGAATGAGCAAGAACGTGATCGATGAAGTAGCGAGAAGAACAGAATCGGAACTCGAGGAAGCAATCAAGGACGGCTATTCGACGACCGATCGAGAAGTATCAGAGGAAGGCGTCAGCGGCGGCGCCTGCTGCGTCGCCGCATTGATCCGGAACGGAAATCTCGCAGTCTCCAACGTAGGCGATTGTCGCGCCGTGTTGAGCAGAAGGGGAAAAGCAGAAGCCCTAACGTCCGATCACCGCCCGTCCAGAGAAGACGAGAGAAATCGGATCGAAAATTCGGGTGGTTATGTAGATTGTTGCCGTGGAGCGTGGAGAGTTCAAGGAACACTAGCAGTGTCGCGAGCGATCGGAGATGGACATTTGAAACAGTGGGTGATATCGGAGCCGGAAACGAGAGTGGTGAAGATTGAGGATGATTGCGATTTCTTGATTCTTGCTTCTGATGGTCTTTGGGACAAGGTTACGAATCAGGAAGCGGTAGATATAGTGCAGCCGCTGTGCGTAGGGGCGGAGAAGCCGAAACTATTCTCGGCGTGTAAAGAACTCGCCGCCTTGTCCACATCGAGAGGCTCAATCGACGATACGAGCGTCATGATCATCAAATTACCATCCTTTATCTGA

Protein sequence

MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPARIAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEAVDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI
Homology
BLAST of Moc09g32900 vs. NCBI nr
Match: XP_022146753.1 (probable protein phosphatase 2C 25 [Momordica charantia])

HSP 1 Score: 668.7 bits (1724), Expect = 2.7e-188
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR 60
           MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR
Sbjct: 17  MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR 76

Query: 61  IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 120
           IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR
Sbjct: 77  IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 136

Query: 121 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 180
           QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS
Sbjct: 137 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 196

Query: 181 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 240
           GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC
Sbjct: 197 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 256

Query: 241 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 300
           CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA
Sbjct: 257 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 316

Query: 301 VDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
           VDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI
Sbjct: 317 VDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 363

BLAST of Moc09g32900 vs. NCBI nr
Match: XP_022995964.1 (probable protein phosphatase 2C 25 [Cucurbita maxima])

HSP 1 Score: 528.5 bits (1360), Expect = 4.4e-146
Identity = 292/362 (80.66%), Postives = 311/362 (85.91%), Query Frame = 0

Query: 1   MVSLLYNKS--------SLNCSRSSSSPRSVSSPRKIHVSPSSP-----PPPCQPSVLPL 60
           + SLLYN S        S + S SSSSPRSVSSPRK+HVS SSP      P   PSV PL
Sbjct: 23  IASLLYNSSLNYCCSSPSSSSSSSSSSPRSVSSPRKLHVSASSPSLLPSTPQLPPSVSPL 82

Query: 61  AVDTVLKRKRPARIAVPVISSAAVSF-GFGFESEKVEAMEEEGEGYSVYCKRGRRRSAME 120
           AVDTVLKRKRPARI VP     ++ F GFGF  E+ E MEEE E YSVYCKRGRRR A+E
Sbjct: 83  AVDTVLKRKRPARINVP----QSIDFIGFGF--EQPEVMEEEEEEYSVYCKRGRRRIAVE 142

Query: 121 DRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDG 180
           DRFSA+VGI+GDSRQAFFGV+DGHGGAKVAEIAAKRMS+NVID+V RRT+SELEEAIKDG
Sbjct: 143 DRFSATVGIQGDSRQAFFGVYDGHGGAKVAEIAAKRMSENVIDQVMRRTDSELEEAIKDG 202

Query: 181 YSTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSRED 240
           Y TTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRP RED
Sbjct: 203 YLTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPCRED 262

Query: 241 ERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLIL 300
           ERNRIENSGGY+D CRGAWRVQGTLAVSRAIGD HLKQWVISEPETR+VKIEDDCDFLIL
Sbjct: 263 ERNRIENSGGYIDYCRGAWRVQGTLAVSRAIGDDHLKQWVISEPETRMVKIEDDCDFLIL 322

Query: 301 ASDGLWDKVTNQEAVDIVQPLC-VGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPS 348
           ASDG WDKV NQEAVDIVQ LC V  EKPKLF+ACKELAALSTSRGS+DDT+VMIIKL S
Sbjct: 323 ASDGFWDKVKNQEAVDIVQSLCGVEMEKPKLFAACKELAALSTSRGSVDDTTVMIIKLSS 378

BLAST of Moc09g32900 vs. NCBI nr
Match: XP_023526748.1 (probable protein phosphatase 2C 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.9 bits (1343), Expect = 4.1e-144
Identity = 292/362 (80.66%), Postives = 310/362 (85.64%), Query Frame = 0

Query: 1   MVSLLYNKSSLN--------CSRSSSSPRSVSSPRKIHVSPSSP-----PPPCQPSVLPL 60
           + SLLY KSSLN         S SSSSPRSVSSPRK+HVS SSP      P   PSV PL
Sbjct: 23  IASLLY-KSSLNYCCSSPSSSSSSSSSPRSVSSPRKLHVSASSPSLLPSTPQLPPSVSPL 82

Query: 61  AVDTVLKRKRPARIAVPVISSAAVSF-GFGFESEKVEAMEEEGEGYSVYCKRGRRRSAME 120
           AVDTVLKRKRPARI V    + ++ F GFGF  E+ E MEEE E YSVYCKRGRRR A+E
Sbjct: 83  AVDTVLKRKRPARINV----TQSIDFIGFGF--EQPEVMEEEEEEYSVYCKRGRRRIAVE 142

Query: 121 DRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDG 180
           DRFSA+VGI+GDSRQAFFGV+DGHGGAKVAEIAAKRMS+NVID+V RRT+SELEEAIKDG
Sbjct: 143 DRFSATVGIQGDSRQAFFGVYDGHGGAKVAEIAAKRMSENVIDQVTRRTDSELEEAIKDG 202

Query: 181 YSTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSRED 240
           Y  TDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRP RED
Sbjct: 203 YLATDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPCRED 262

Query: 241 ERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLIL 300
           ERNRIEN GGYVD CRGAWRVQGTLAVSRAIGD HLKQWVISEPETR+VKIEDDCDFLIL
Sbjct: 263 ERNRIENLGGYVDYCRGAWRVQGTLAVSRAIGDDHLKQWVISEPETRMVKIEDDCDFLIL 322

Query: 301 ASDGLWDKVTNQEAVDIVQPLC-VGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPS 348
           ASDG WDKV NQEAVDIVQ LC V  EKPKLF+ACKELAALSTSRGS+DDT+VMIIKL S
Sbjct: 323 ASDGFWDKVKNQEAVDIVQSLCGVEMEKPKLFAACKELAALSTSRGSVDDTTVMIIKLSS 377

BLAST of Moc09g32900 vs. NCBI nr
Match: KAG7030663.1 (putative protein phosphatase 2C 25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 519.6 bits (1337), Expect = 2.0e-143
Identity = 285/348 (81.90%), Postives = 303/348 (87.07%), Query Frame = 0

Query: 7   NKSSLNCSRSSSSPRSVSSPRKIHVSPSS-----PPPPCQPSVLPLAVDTVLKRKRPARI 66
           + SS + S SSSSPRSVSSPRK+HVS SS       P   PSV PLAVDTVLKRKRPARI
Sbjct: 41  SSSSSSSSSSSSSPRSVSSPRKLHVSASSTSLLPSTPQLPPSVSPLAVDTVLKRKRPARI 100

Query: 67  AVPVISSAAVSF-GFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 126
            VP     ++ F GFGF  E+ E MEEE E YSVYCKRGRRR A+EDRFSA+VGI+GDSR
Sbjct: 101 NVP----QSIDFIGFGF--EQPEVMEEEEEEYSVYCKRGRRRIAVEDRFSATVGIQGDSR 160

Query: 127 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 186
           QAFFGV+DGHGGAKVAEIAAKRMS+NVID+V RRT+SELEEAIKDGY  TDREVSEEGVS
Sbjct: 161 QAFFGVYDGHGGAKVAEIAAKRMSENVIDQVTRRTDSELEEAIKDGYLATDREVSEEGVS 220

Query: 187 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 246
           GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRP REDERNRIEN GGYVD 
Sbjct: 221 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPCREDERNRIENLGGYVDY 280

Query: 247 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 306
           CRGAWRVQGTLAVSRAIGD HLKQWVISEPETR+VKIEDDCDFLILASDG WDKV NQEA
Sbjct: 281 CRGAWRVQGTLAVSRAIGDDHLKQWVISEPETRMVKIEDDCDFLILASDGFWDKVKNQEA 340

Query: 307 VDIVQPLC-VGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
           VDIVQ LC V  EKPKLF+ACKELAALSTSRGS+DDT+VMIIKL SF+
Sbjct: 341 VDIVQSLCGVEMEKPKLFAACKELAALSTSRGSVDDTTVMIIKLSSFL 382

BLAST of Moc09g32900 vs. NCBI nr
Match: XP_022941656.1 (putative protein phosphatase 2C 53 [Cucurbita moschata])

HSP 1 Score: 519.6 bits (1337), Expect = 2.0e-143
Identity = 285/348 (81.90%), Postives = 303/348 (87.07%), Query Frame = 0

Query: 7   NKSSLNCSRSSSSPRSVSSPRKIHVSPSS-----PPPPCQPSVLPLAVDTVLKRKRPARI 66
           + SS + S SSSSPRSVSSPRK+HVS SS       P   PSV PLAVDTVLKRKRPARI
Sbjct: 40  SSSSSSSSSSSSSPRSVSSPRKLHVSASSTSLLPSTPQLPPSVSPLAVDTVLKRKRPARI 99

Query: 67  AVPVISSAAVSF-GFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 126
            VP     ++ F GFGF  E+ E MEEE E YSVYCKRGRRR A+EDRFSA+VGI+GDSR
Sbjct: 100 NVP----QSIDFIGFGF--EQPEVMEEEEEEYSVYCKRGRRRIAVEDRFSATVGIQGDSR 159

Query: 127 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 186
           QAFFGV+DGHGGAKVAEIAAKRMS+NVID+V RRT+SELEEAIKDGY  TDREVSEEGVS
Sbjct: 160 QAFFGVYDGHGGAKVAEIAAKRMSENVIDQVTRRTDSELEEAIKDGYLATDREVSEEGVS 219

Query: 187 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 246
           GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRP REDERNRIEN GGYVD 
Sbjct: 220 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPCREDERNRIENLGGYVDY 279

Query: 247 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 306
           CRGAWRVQGTLAVSRAIGD HLKQWVISEPETR+VKIEDDCDFLILASDG WDKV NQEA
Sbjct: 280 CRGAWRVQGTLAVSRAIGDDHLKQWVISEPETRMVKIEDDCDFLILASDGFWDKVKNQEA 339

Query: 307 VDIVQPLC-VGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
           VDIVQ LC V  EKPKLF+ACKELAALSTSRGS+DDT+VMIIKL SF+
Sbjct: 340 VDIVQSLCGVEMEKPKLFAACKELAALSTSRGSVDDTTVMIIKLSSFL 381

BLAST of Moc09g32900 vs. ExPASy Swiss-Prot
Match: O80871 (Probable protein phosphatase 2C 25 OS=Arabidopsis thaliana OX=3702 GN=At2g30020 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 200/367 (54.50%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 2   VSLLYNKSSLNCSRSSSSPRSVSSPRK-IHVSPSSPPPPCQPSVLPLAVDT--------- 61
           +SL  +      S  SS   +VSSP+    +    PP    P  L    ++         
Sbjct: 34  LSLTLSHRKPQTSSPSSPSTTVSSPKSPFRLRFQKPPSGFAPGPLSFGSESVSASSPPGG 93

Query: 62  VLKRKRPARIAV---------PVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRR 121
           VLKRKRP R+ +         P+ SSAAV+       E+   +E EG+GYSVYCKRG RR
Sbjct: 94  VLKRKRPTRLDIPIGVAGFVAPISSSAAVA---ATPREECREVEREGDGYSVYCKRG-RR 153

Query: 122 SAMEDRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDE-VARRTESELEE 181
            AMEDRFSA   + GD +QA FGV+DGHGG K AE AAK + KN+++E V +R ESE+ E
Sbjct: 154 EAMEDRFSAITNLHGDRKQAIFGVYDGHGGVKAAEFAAKNLDKNIVEEVVGKRDESEIAE 213

Query: 182 AIKDGYSTTDRE-VSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDH 241
           A+K GY  TD   + EE V GG+CCV AL+  GNL VSN GDCRAV+S  G A+AL+SDH
Sbjct: 214 AVKHGYLATDASFLKEEDVKGGSCCVTALVNEGNLVVSNAGDCRAVMSVGGVAKALSSDH 273

Query: 242 RPSREDERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDD 301
           RPSR+DER RIE +GGYVD   G WR+QG+LAVSR IGD  LK+WVI+EPET++ +IE D
Sbjct: 274 RPSRDDERKRIETTGGYVDTFHGVWRIQGSLAVSRGIGDAQLKKWVIAEPETKISRIEHD 333

Query: 302 CDFLILASDGLWDKVTNQEAVDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMI 348
            +FLILASDGLWDKV+NQEAVDI +PLC+G EKP L +ACK+L  LS SRGS DD SVM+
Sbjct: 334 HEFLILASDGLWDKVSNQEAVDIARPLCLGTEKPLLLAACKKLVDLSASRGSSDDISVML 393

BLAST of Moc09g32900 vs. ExPASy Swiss-Prot
Match: Q8RX37 (Probable protein phosphatase 2C 2 OS=Arabidopsis thaliana OX=3702 GN=At1g07160 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.6e-90
Identity = 192/346 (55.49%), Postives = 236/346 (68.21%), Query Frame = 0

Query: 3   SLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPARIA 62
           +L  + S LN   SS+SP S +SP          PP            ++LKRKRP  + 
Sbjct: 33  TLTLSLSHLNPPVSSTSP-SAASPTSPFCLRLLKPPAKLGFGSDSGPGSILKRKRPTTLD 92

Query: 63  VPV--ISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 122
           +PV  +  AA         E+  A+E EG+GYSVYCKRG +R AMEDRFSA   ++GD +
Sbjct: 93  IPVAPVGIAAPISNADTPREESRAVEREGDGYSVYCKRG-KREAMEDRFSAITNLQGDPK 152

Query: 123 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDE-VARRTESELEEAIKDGYSTTDRE-VSEEG 182
           QA FGV+DGHGG   AE AAK +  N++ E V  R ES++EEA+K GY  TD E + E+ 
Sbjct: 153 QAIFGVYDGHGGPTAAEFAAKNLCSNILGEIVGGRNESKIEEAVKRGYLATDSEFLKEKN 212

Query: 183 VSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYV 242
           V GG+CCV ALI +GNL V+N GDCRAVLS  G AEALTSDHRPSR+DERNRIE+SGGYV
Sbjct: 213 VKGGSCCVTALISDGNLVVANAGDCRAVLSVGGFAEALTSDHRPSRDDERNRIESSGGYV 272

Query: 243 DCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQ 302
           D     WR+QG+LAVSR IGD HLKQW+ISEPE  +++I    +FLILASDGLWDKV+NQ
Sbjct: 273 DTFNSVWRIQGSLAVSRGIGDAHLKQWIISEPEINILRINPQHEFLILASDGLWDKVSNQ 332

Query: 303 EAVDIVQPLCVGA-EKPKLFSACKELAALSTSRGSIDDTSVMIIKL 344
           EAVDI +P C G  +K K   ACK+L  LS SRGS+DD SVM+I+L
Sbjct: 333 EAVDIARPFCKGTDQKRKPLLACKKLVDLSVSRGSLDDISVMLIQL 376

BLAST of Moc09g32900 vs. ExPASy Swiss-Prot
Match: Q9XEE8 (Probable protein phosphatase 2C 30 OS=Arabidopsis thaliana OX=3702 GN=PP2C5 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.3e-87
Identity = 189/341 (55.43%), Postives = 232/341 (68.04%), Query Frame = 0

Query: 18  SSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPARI---AVPVISSAAVSFG 77
           SSPR  SSP K+ +    P  P +        +TVLKRKRP  +   A P ++S   +  
Sbjct: 54  SSPR--SSPPKLTMVACPPRKPKETKTTGSDSETVLKRKRPPMLDLTAAPTVASWCSTTR 113

Query: 78  FGFE--SEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDS--RQAFFGVFDGH 137
              E  +E VEA EE+G  YSVYCKRG RR  MEDR+ A+V    D   + AFFGVFDGH
Sbjct: 114 ETAEKGAEVVEA-EEDGY-YSVYCKRG-RRGPMEDRYFAAVDRNDDGGYKNAFFGVFDGH 173

Query: 138 GGAKVAEIAAKRMSKNVIDEVARRTESE----LEEAIKDGYSTTDREVSEEGVSGGACCV 197
           GG+K AE AA  +  N+   +A     E    +E AI++GY  TD +  +EG  GGACCV
Sbjct: 174 GGSKAAEFAAMNLGNNIEAAMASARSGEDGCSMESAIREGYIKTDEDFLKEGSRGGACCV 233

Query: 198 AALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDCCRGAWR 257
            ALI  G LAVSN GDCRAV+SR G AEALTSDH PS+ +E  RIE  GGYVDCC G WR
Sbjct: 234 TALISKGELAVSNAGDCRAVMSRGGTAEALTSDHNPSQANELKRIEALGGYVDCCNGVWR 293

Query: 258 VQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEAVDIVQP 317
           +QGTLAVSR IGD +LK+WVI+EPETR ++I+ + +FLILASDGLWDKVTNQEAVD+V+P
Sbjct: 294 IQGTLAVSRGIGDRYLKEWVIAEPETRTLRIKPEFEFLILASDGLWDKVTNQEAVDVVRP 353

Query: 318 LCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
            CVG E P   SACK+LA LS  RGS+DD S++II+L +F+
Sbjct: 354 YCVGVENPMTLSACKKLAELSVKRGSLDDISLIIIQLQNFL 389

BLAST of Moc09g32900 vs. ExPASy Swiss-Prot
Match: Q10MX1 (Probable protein phosphatase 2C 32 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0292100 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.8e-84
Identity = 171/336 (50.89%), Postives = 219/336 (65.18%), Query Frame = 0

Query: 20  PRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPARIAVPVISSAAVSFG----F 79
           P ++ +  +   +PS  P     + +     +VLKR+RPA + VPV   AA +       
Sbjct: 54  PFALRAHLREEATPSPQPSAAAAAAVSAPAGSVLKRRRPAPLVVPVCGGAAAAAAAAAVA 113

Query: 80  GFESEKVEAMEEEGEGYSVYCKR--GRRRSAMEDRFSASVGIRGDSRQAFFGVFDGHGGA 139
             ES+    +EE+GE ++VYC+R  GRRR  MEDR  A V + GD + AFFGVFDGHGG 
Sbjct: 114 AVESDPRNEVEEDGEEFAVYCRRGKGRRRVEMEDRHVAKVALGGDPKVAFFGVFDGHGGK 173

Query: 140 KVAEIAAKRMSKNVIDEVAR---RTESELEEAIKDGYSTTDREVSEEGVSGGACCVAALI 199
             AE  A+ M K + +E+ +       E E+A+K  Y  TD E  +   SGGACCV AL+
Sbjct: 174 SAAEFVAENMPKFMAEEMCKVDGGDSGETEQAVKRCYLKTDEEFLKREESGGACCVTALL 233

Query: 200 RNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDCCRGAWRVQGT 259
           + G L VSN GDCRAVLSR GKAEALTSDHR SREDER RIEN GG+V   RG WRVQG+
Sbjct: 234 QKGGLVVSNAGDCRAVLSRAGKAEALTSDHRASREDERERIENLGGFVVNYRGTWRVQGS 293

Query: 260 LAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEAVDIVQPLCVG 319
           LAVSR IGD HLKQWV+S+P+T  + ++  C+FLILASDGLWDKV NQEAVDI +PL + 
Sbjct: 294 LAVSRGIGDAHLKQWVVSDPDTTTLGVDSQCEFLILASDGLWDKVENQEAVDIARPLYIS 353

Query: 320 AEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSF 347
            +K    +AC+ L   + +RGS DD S++II+L  F
Sbjct: 354 NDKASRMTACRRLVETAVTRGSTDDISIVIIQLQQF 389

BLAST of Moc09g32900 vs. ExPASy Swiss-Prot
Match: Q2QWE3 (Probable protein phosphatase 2C 77 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0198200 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.7e-63
Identity = 168/394 (42.64%), Postives = 213/394 (54.06%), Query Frame = 0

Query: 13  CSRSSSSPRSVSSP----------RKIHVSPSS---PPPPCQPS-VLPL----------- 72
           CS   S+   + SP          +K+H+       PPPP  P+ VLP            
Sbjct: 27  CSPKFSTAGLIGSPTTAWGGGGAKKKVHMESEKQQLPPPPSPPALVLPAFKRNCTDQEQR 86

Query: 73  --AVDTVLKRKRPARIAVPVISSAAVSFG-----FGFESEKVEAMEEEGEGYSVYCKRGR 132
             A  T   R RP R+ +P  +  AV+ G      G E++     E +GEG+ +  +RG 
Sbjct: 87  APATSTKAARGRPPRLVIPAAAPVAVARGGVDPFGGRETDVATETEVKGEGFCLASRRGV 146

Query: 133 RRSAMEDRFSASV--GIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVA------ 192
           R  AMED +       I G S+ AF+GV+DGHGG    +  A ++ KNV+   A      
Sbjct: 147 RH-AMEDGYGVITRHKIEGGSQLAFYGVYDGHGGRAAVDFVADKLGKNVVTAAAAATTMS 206

Query: 193 --------------RRTESELEEAIKDGYSTTDREVSEEGVSGGACCVAALIRNGNLAVS 252
                         R  E ++  AI+  Y TTD E   +GV GGAC   AL+++G L VS
Sbjct: 207 RHQAAGSSSPSQQRREEEDDVTAAIRAAYLTTDSEFLSQGVRGGACAATALVKDGELYVS 266

Query: 253 NVGDCRAVL-SRRGKAEALTSDHRPSREDERNRIENSGGYVDC-CRGAWRVQGTLAVSRA 312
           NVGDCRAVL SR G A ALTSDH P REDER RIE++GGYV C   G WRVQ +LAVSRA
Sbjct: 267 NVGDCRAVLGSRGGVATALTSDHTPGREDERLRIESTGGYVSCGGSGVWRVQDSLAVSRA 326

Query: 313 IGDGHLKQWVISEPETRVVKI--EDDCDFLILASDGLWDKVTNQEAVDIVQPLCVGAE-K 348
            GD  +KQWV  EPET  V +  + DC FL+LASDGLW KV+NQEAVD V      A   
Sbjct: 327 FGDAGVKQWVTCEPETARVSLAADGDCRFLVLASDGLWCKVSNQEAVDAVAAAAAAAAGV 386

BLAST of Moc09g32900 vs. ExPASy TrEMBL
Match: A0A6J1CZE1 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111015885 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.3e-188
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR 60
           MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR
Sbjct: 17  MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR 76

Query: 61  IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 120
           IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR
Sbjct: 77  IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 136

Query: 121 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 180
           QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS
Sbjct: 137 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 196

Query: 181 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 240
           GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC
Sbjct: 197 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 256

Query: 241 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 300
           CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA
Sbjct: 257 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 316

Query: 301 VDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
           VDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI
Sbjct: 317 VDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 363

BLAST of Moc09g32900 vs. ExPASy TrEMBL
Match: A0A6J1K7E6 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111491317 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.1e-146
Identity = 292/362 (80.66%), Postives = 311/362 (85.91%), Query Frame = 0

Query: 1   MVSLLYNKS--------SLNCSRSSSSPRSVSSPRKIHVSPSSP-----PPPCQPSVLPL 60
           + SLLYN S        S + S SSSSPRSVSSPRK+HVS SSP      P   PSV PL
Sbjct: 23  IASLLYNSSLNYCCSSPSSSSSSSSSSPRSVSSPRKLHVSASSPSLLPSTPQLPPSVSPL 82

Query: 61  AVDTVLKRKRPARIAVPVISSAAVSF-GFGFESEKVEAMEEEGEGYSVYCKRGRRRSAME 120
           AVDTVLKRKRPARI VP     ++ F GFGF  E+ E MEEE E YSVYCKRGRRR A+E
Sbjct: 83  AVDTVLKRKRPARINVP----QSIDFIGFGF--EQPEVMEEEEEEYSVYCKRGRRRIAVE 142

Query: 121 DRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDG 180
           DRFSA+VGI+GDSRQAFFGV+DGHGGAKVAEIAAKRMS+NVID+V RRT+SELEEAIKDG
Sbjct: 143 DRFSATVGIQGDSRQAFFGVYDGHGGAKVAEIAAKRMSENVIDQVMRRTDSELEEAIKDG 202

Query: 181 YSTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSRED 240
           Y TTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRP RED
Sbjct: 203 YLTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPCRED 262

Query: 241 ERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLIL 300
           ERNRIENSGGY+D CRGAWRVQGTLAVSRAIGD HLKQWVISEPETR+VKIEDDCDFLIL
Sbjct: 263 ERNRIENSGGYIDYCRGAWRVQGTLAVSRAIGDDHLKQWVISEPETRMVKIEDDCDFLIL 322

Query: 301 ASDGLWDKVTNQEAVDIVQPLC-VGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPS 348
           ASDG WDKV NQEAVDIVQ LC V  EKPKLF+ACKELAALSTSRGS+DDT+VMIIKL S
Sbjct: 323 ASDGFWDKVKNQEAVDIVQSLCGVEMEKPKLFAACKELAALSTSRGSVDDTTVMIIKLSS 378

BLAST of Moc09g32900 vs. ExPASy TrEMBL
Match: A0A6J1FLP9 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111446952 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.9e-144
Identity = 285/348 (81.90%), Postives = 303/348 (87.07%), Query Frame = 0

Query: 7   NKSSLNCSRSSSSPRSVSSPRKIHVSPSS-----PPPPCQPSVLPLAVDTVLKRKRPARI 66
           + SS + S SSSSPRSVSSPRK+HVS SS       P   PSV PLAVDTVLKRKRPARI
Sbjct: 40  SSSSSSSSSSSSSPRSVSSPRKLHVSASSTSLLPSTPQLPPSVSPLAVDTVLKRKRPARI 99

Query: 67  AVPVISSAAVSF-GFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 126
            VP     ++ F GFGF  E+ E MEEE E YSVYCKRGRRR A+EDRFSA+VGI+GDSR
Sbjct: 100 NVP----QSIDFIGFGF--EQPEVMEEEEEEYSVYCKRGRRRIAVEDRFSATVGIQGDSR 159

Query: 127 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGVS 186
           QAFFGV+DGHGGAKVAEIAAKRMS+NVID+V RRT+SELEEAIKDGY  TDREVSEEGVS
Sbjct: 160 QAFFGVYDGHGGAKVAEIAAKRMSENVIDQVTRRTDSELEEAIKDGYLATDREVSEEGVS 219

Query: 187 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 246
           GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRP REDERNRIEN GGYVD 
Sbjct: 220 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPCREDERNRIENLGGYVDY 279

Query: 247 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 306
           CRGAWRVQGTLAVSRAIGD HLKQWVISEPETR+VKIEDDCDFLILASDG WDKV NQEA
Sbjct: 280 CRGAWRVQGTLAVSRAIGDDHLKQWVISEPETRMVKIEDDCDFLILASDGFWDKVKNQEA 339

Query: 307 VDIVQPLC-VGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
           VDIVQ LC V  EKPKLF+ACKELAALSTSRGS+DDT+VMIIKL SF+
Sbjct: 340 VDIVQSLCGVEMEKPKLFAACKELAALSTSRGSVDDTTVMIIKLSSFL 381

BLAST of Moc09g32900 vs. ExPASy TrEMBL
Match: A0A6J1JG01 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111484158 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.3e-130
Identity = 265/353 (75.07%), Postives = 283/353 (80.17%), Query Frame = 0

Query: 1   MVSLLYNKSSLNCSRS--SSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRP 60
           + S  Y   SLN S    SS  RSVSSP  +   P               VDTVLKRKRP
Sbjct: 21  VASPFYKSYSLNYSSPSYSSLSRSVSSPPSVSSLPD--------------VDTVLKRKRP 80

Query: 61  ARIAVPVISSAAVSFGFGFES-EKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRG 120
           ARI VP I     S GFGF + E  E MEEEGEGYSVYCKRGRRRSAMEDRFSA+VGI+G
Sbjct: 81  ARIDVPTI---VPSIGFGFGTFETPEVMEEEGEGYSVYCKRGRRRSAMEDRFSATVGIQG 140

Query: 121 DSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEE 180
           DSRQAFFG+FDGHGGA+VAEIAAKRMS+NVID+V RRTESELEEAIKDGY TTDREVSEE
Sbjct: 141 DSRQAFFGIFDGHGGAQVAEIAAKRMSENVIDQVTRRTESELEEAIKDGYLTTDREVSEE 200

Query: 181 GVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGY 240
           GVSGGAC   ALIRNG+LAVSNVGDCRAVLSR+GKAEALTSDHR  REDERNRIE SGGY
Sbjct: 201 GVSGGACSATALIRNGHLAVSNVGDCRAVLSRKGKAEALTSDHRACREDERNRIEKSGGY 260

Query: 241 VDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTN 300
           VDCCRGAWRVQGTLAVSRAIGD HLKQWVISEPETRVVKIEDDCD+LILASDGLWDK+TN
Sbjct: 261 VDCCRGAWRVQGTLAVSRAIGDKHLKQWVISEPETRVVKIEDDCDYLILASDGLWDKITN 320

Query: 301 QEAVDIVQPLCVGAE-------KPKLFSACKELAALSTSRGSIDDTSVMIIKL 344
           QEAVD+V+  C G E        P L SACK+LAALS SRGS+DDT+VMIIKL
Sbjct: 321 QEAVDVVEAAC-GVEIMENKKPNPLLMSACKQLAALSASRGSVDDTTVMIIKL 355

BLAST of Moc09g32900 vs. ExPASy TrEMBL
Match: A0A5D3E334 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold344G00590 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.4e-129
Identity = 267/352 (75.85%), Postives = 288/352 (81.82%), Query Frame = 0

Query: 1   MVSLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPAR 60
           + SLL   SSLN S SSSSPRS+S+P  +HVS SS P P   S     VDTVLKRKRPAR
Sbjct: 10  IASLLCKSSSLNSSTSSSSPRSLSTPMNLHVSASSSPSPSSVS----HVDTVLKRKRPAR 69

Query: 61  IAVPVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRR-SAMEDRFSASVGIRGDS 120
           I VP    +  S GFG   E  E MEEEGEGYSVYCKRGRRR SAMEDRFS SVGI+GDS
Sbjct: 70  IHVP---HSMPSIGFGI-LETPEVMEEEGEGYSVYCKRGRRRISAMEDRFSVSVGIQGDS 129

Query: 121 RQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIKDGYSTTDREVSEEGV 180
           RQAFFGVFDGHGGAKVAEIAAKR+S+NVID+V RRTESE+EEAIKDGY  TDREVSEEG 
Sbjct: 130 RQAFFGVFDGHGGAKVAEIAAKRLSENVIDQVWRRTESEVEEAIKDGYLMTDREVSEEG- 189

Query: 181 SGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVD 240
            GGACCV ALIRNGNLAVSNVGDCRAVLSR G+AEALTSDHR  REDERNRIE SGGYVD
Sbjct: 190 -GGACCVTALIRNGNLAVSNVGDCRAVLSRNGRAEALTSDHRAGREDERNRIEKSGGYVD 249

Query: 241 CCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQE 300
           CC G WRVQGTLAVSRA+GD HLKQWVISEPE+RV+KIEDDC FLILASDGLWDKVTNQE
Sbjct: 250 CCGGGWRVQGTLAVSRAMGDEHLKQWVISEPESRVMKIEDDCRFLILASDGLWDKVTNQE 309

Query: 301 AVDIVQPLCVGAE-------KPKL-FSACKELAALSTSRGSIDDTSVMIIKL 344
           AVD+V+ +C G E        PKL  SACK+L  LSTSRGS+DDT+VMII+L
Sbjct: 310 AVDMVEAVC-GVEIKKNRPINPKLIMSACKQLVTLSTSRGSLDDTTVMIIQL 350

BLAST of Moc09g32900 vs. TAIR 10
Match: AT2G30020.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 351.3 bits (900), Expect = 9.0e-97
Identity = 200/367 (54.50%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 2   VSLLYNKSSLNCSRSSSSPRSVSSPRK-IHVSPSSPPPPCQPSVLPLAVDT--------- 61
           +SL  +      S  SS   +VSSP+    +    PP    P  L    ++         
Sbjct: 34  LSLTLSHRKPQTSSPSSPSTTVSSPKSPFRLRFQKPPSGFAPGPLSFGSESVSASSPPGG 93

Query: 62  VLKRKRPARIAV---------PVISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRR 121
           VLKRKRP R+ +         P+ SSAAV+       E+   +E EG+GYSVYCKRG RR
Sbjct: 94  VLKRKRPTRLDIPIGVAGFVAPISSSAAVA---ATPREECREVEREGDGYSVYCKRG-RR 153

Query: 122 SAMEDRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDE-VARRTESELEE 181
            AMEDRFSA   + GD +QA FGV+DGHGG K AE AAK + KN+++E V +R ESE+ E
Sbjct: 154 EAMEDRFSAITNLHGDRKQAIFGVYDGHGGVKAAEFAAKNLDKNIVEEVVGKRDESEIAE 213

Query: 182 AIKDGYSTTDRE-VSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDH 241
           A+K GY  TD   + EE V GG+CCV AL+  GNL VSN GDCRAV+S  G A+AL+SDH
Sbjct: 214 AVKHGYLATDASFLKEEDVKGGSCCVTALVNEGNLVVSNAGDCRAVMSVGGVAKALSSDH 273

Query: 242 RPSREDERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDD 301
           RPSR+DER RIE +GGYVD   G WR+QG+LAVSR IGD  LK+WVI+EPET++ +IE D
Sbjct: 274 RPSRDDERKRIETTGGYVDTFHGVWRIQGSLAVSRGIGDAQLKKWVIAEPETKISRIEHD 333

Query: 302 CDFLILASDGLWDKVTNQEAVDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMI 348
            +FLILASDGLWDKV+NQEAVDI +PLC+G EKP L +ACK+L  LS SRGS DD SVM+
Sbjct: 334 HEFLILASDGLWDKVSNQEAVDIARPLCLGTEKPLLLAACKKLVDLSASRGSSDDISVML 393

BLAST of Moc09g32900 vs. TAIR 10
Match: AT1G07160.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.3e-91
Identity = 192/346 (55.49%), Postives = 236/346 (68.21%), Query Frame = 0

Query: 3   SLLYNKSSLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPARIA 62
           +L  + S LN   SS+SP S +SP          PP            ++LKRKRP  + 
Sbjct: 33  TLTLSLSHLNPPVSSTSP-SAASPTSPFCLRLLKPPAKLGFGSDSGPGSILKRKRPTTLD 92

Query: 63  VPV--ISSAAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSR 122
           +PV  +  AA         E+  A+E EG+GYSVYCKRG +R AMEDRFSA   ++GD +
Sbjct: 93  IPVAPVGIAAPISNADTPREESRAVEREGDGYSVYCKRG-KREAMEDRFSAITNLQGDPK 152

Query: 123 QAFFGVFDGHGGAKVAEIAAKRMSKNVIDE-VARRTESELEEAIKDGYSTTDRE-VSEEG 182
           QA FGV+DGHGG   AE AAK +  N++ E V  R ES++EEA+K GY  TD E + E+ 
Sbjct: 153 QAIFGVYDGHGGPTAAEFAAKNLCSNILGEIVGGRNESKIEEAVKRGYLATDSEFLKEKN 212

Query: 183 VSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYV 242
           V GG+CCV ALI +GNL V+N GDCRAVLS  G AEALTSDHRPSR+DERNRIE+SGGYV
Sbjct: 213 VKGGSCCVTALISDGNLVVANAGDCRAVLSVGGFAEALTSDHRPSRDDERNRIESSGGYV 272

Query: 243 DCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQ 302
           D     WR+QG+LAVSR IGD HLKQW+ISEPE  +++I    +FLILASDGLWDKV+NQ
Sbjct: 273 DTFNSVWRIQGSLAVSRGIGDAHLKQWIISEPEINILRINPQHEFLILASDGLWDKVSNQ 332

Query: 303 EAVDIVQPLCVGA-EKPKLFSACKELAALSTSRGSIDDTSVMIIKL 344
           EAVDI +P C G  +K K   ACK+L  LS SRGS+DD SVM+I+L
Sbjct: 333 EAVDIARPFCKGTDQKRKPLLACKKLVDLSVSRGSLDDISVMLIQL 376

BLAST of Moc09g32900 vs. TAIR 10
Match: AT2G40180.1 (phosphatase 2C5 )

HSP 1 Score: 324.7 bits (831), Expect = 9.0e-89
Identity = 189/341 (55.43%), Postives = 232/341 (68.04%), Query Frame = 0

Query: 18  SSPRSVSSPRKIHVSPSSPPPPCQPSVLPLAVDTVLKRKRPARI---AVPVISSAAVSFG 77
           SSPR  SSP K+ +    P  P +        +TVLKRKRP  +   A P ++S   +  
Sbjct: 54  SSPR--SSPPKLTMVACPPRKPKETKTTGSDSETVLKRKRPPMLDLTAAPTVASWCSTTR 113

Query: 78  FGFE--SEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDS--RQAFFGVFDGH 137
              E  +E VEA EE+G  YSVYCKRG RR  MEDR+ A+V    D   + AFFGVFDGH
Sbjct: 114 ETAEKGAEVVEA-EEDGY-YSVYCKRG-RRGPMEDRYFAAVDRNDDGGYKNAFFGVFDGH 173

Query: 138 GGAKVAEIAAKRMSKNVIDEVARRTESE----LEEAIKDGYSTTDREVSEEGVSGGACCV 197
           GG+K AE AA  +  N+   +A     E    +E AI++GY  TD +  +EG  GGACCV
Sbjct: 174 GGSKAAEFAAMNLGNNIEAAMASARSGEDGCSMESAIREGYIKTDEDFLKEGSRGGACCV 233

Query: 198 AALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDCCRGAWR 257
            ALI  G LAVSN GDCRAV+SR G AEALTSDH PS+ +E  RIE  GGYVDCC G WR
Sbjct: 234 TALISKGELAVSNAGDCRAVMSRGGTAEALTSDHNPSQANELKRIEALGGYVDCCNGVWR 293

Query: 258 VQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEAVDIVQP 317
           +QGTLAVSR IGD +LK+WVI+EPETR ++I+ + +FLILASDGLWDKVTNQEAVD+V+P
Sbjct: 294 IQGTLAVSRGIGDRYLKEWVIAEPETRTLRIKPEFEFLILASDGLWDKVTNQEAVDVVRP 353

Query: 318 LCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLPSFI 348
            CVG E P   SACK+LA LS  RGS+DD S++II+L +F+
Sbjct: 354 YCVGVENPMTLSACKKLAELSVKRGSLDDISLIIIQLQNFL 389

BLAST of Moc09g32900 vs. TAIR 10
Match: AT1G67820.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.2e-62
Identity = 138/315 (43.81%), Postives = 195/315 (61.90%), Query Frame = 0

Query: 10  SLNCSRSSSSPRSVSSPRKIHVSPSSPPPPCQPSVLPL----AVDTVLKRKRPARIAVPV 69
           S+  S +  SP     P  +++   +P  P            A  + LKRKRPA + +P 
Sbjct: 31  SIPLSVTKVSPLKRKRPTHLNIPDLNPQQPISTDYFRFREGDAKVSPLKRKRPAHLNIPD 90

Query: 70  ISS----AAVSFGFGFESEKVEAMEEEGEGYSVYCKRGRRRSAMEDRFSASVGIRGDSRQ 129
           ++         F F   + +   +   G G+ V  + G+++  MED       + G+S++
Sbjct: 91  LNPQQPIRTDYFSFTDFAHQNGTVSFGGNGFGVVSRNGKKK-FMEDTHRIVPCLVGNSKK 150

Query: 130 AFFGVFDGHGGAKVAEIAAKRMSKNVIDEVAR-RTESELEEAIKDGYSTTDREVSEEGVS 189
           +FFGV+DGHGGAK AE  A+ + K V++ +   + + E  EA K  +  TDR+  E+GV 
Sbjct: 151 SFFGVYDGHGGAKAAEFVAENLHKYVVEMMENCKGKEEKVEAFKAAFLRTDRDFLEKGVV 210

Query: 190 GGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSREDERNRIENSGGYVDC 249
            GACCV A+I++  + VSN+GDCRAVL R G AEALT DH+P R+DE+ RIE+ GGYVD 
Sbjct: 211 SGACCVTAVIQDQEMIVSNLGDCRAVLCRAGVAEALTDDHKPGRDDEKERIESQGGYVDN 270

Query: 250 CRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFLILASDGLWDKVTNQEA 309
            +GAWRVQG LAVSR+IGD HLK+WV++EPETRV+++E D +FL+LASDGLWD V+NQEA
Sbjct: 271 HQGAWRVQGILAVSRSIGDAHLKKWVVAEPETRVLELEQDMEFLVLASDGLWDVVSNQEA 330

Query: 310 VDIVQPLCVGAEKPK 316
           V  V  +    + PK
Sbjct: 331 VYTVLHVLAQRKTPK 344

BLAST of Moc09g32900 vs. TAIR 10
Match: AT4G08260.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 219.2 bits (557), Expect = 5.3e-57
Identity = 119/243 (48.97%), Postives = 148/243 (60.91%), Query Frame = 0

Query: 105 MEDRFSASVGIRGDSRQAFFGVFDGHGGAKVAEIAAKRMSKNVIDEVARRTESELEEAIK 164
           MEDRFSA   + GD +QA FGV+ GHGG K AE AAK + KN+++EV   T         
Sbjct: 1   MEDRFSAITNLHGDHKQAIFGVYVGHGGVKAAEFAAKNLDKNIVEEVVDAT--------- 60

Query: 165 DGYSTTDREVSEEGVSGGACCVAALIRNGNLAVSNVGDCRAVLSRRGKAEALTSDHRPSR 224
                    + EEG  GG+ CV AL+  G+L VSN GDCRAV+S     E +       R
Sbjct: 61  --------FLKEEGFKGGSSCVTALVSEGSLVVSNAGDCRAVMS---VGEMMNGKELKPR 120

Query: 225 EDERNRIENSGGYVDCCRGAWRVQGTLAVSRAIGDGHLKQWVISEPETRVVKIEDDCDFL 284
           ED   R              WR+QG+L V R IGD  LK+WVI+EPET++ ++E D +FL
Sbjct: 121 EDMLIRFT-----------LWRIQGSLVVPRGIGDAQLKKWVIAEPETKISRVEHDHEFL 180

Query: 285 ILASDGLWDKVTNQEAVDIVQPLCVGAEKPKLFSACKELAALSTSRGSIDDTSVMIIKLP 344
           ILAS GLWDKV+NQEAVDI +P C+  EKP L +ACK+L  LS SRGS DD SVM+I L 
Sbjct: 181 ILASHGLWDKVSNQEAVDIARPFCLRTEKPLLLAACKKLVDLSASRGSFDDISVMLIPLR 212

Query: 345 SFI 348
            F+
Sbjct: 241 QFV 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146753.12.7e-188100.00probable protein phosphatase 2C 25 [Momordica charantia][more]
XP_022995964.14.4e-14680.66probable protein phosphatase 2C 25 [Cucurbita maxima][more]
XP_023526748.14.1e-14480.66probable protein phosphatase 2C 25 [Cucurbita pepo subsp. pepo][more]
KAG7030663.12.0e-14381.90putative protein phosphatase 2C 25, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022941656.12.0e-14381.90putative protein phosphatase 2C 53 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O808711.3e-9554.50Probable protein phosphatase 2C 25 OS=Arabidopsis thaliana OX=3702 GN=At2g30020 ... [more]
Q8RX374.6e-9055.49Probable protein phosphatase 2C 2 OS=Arabidopsis thaliana OX=3702 GN=At1g07160 P... [more]
Q9XEE81.3e-8755.43Probable protein phosphatase 2C 30 OS=Arabidopsis thaliana OX=3702 GN=PP2C5 PE=2... [more]
Q10MX13.8e-8450.89Probable protein phosphatase 2C 32 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q2QWE33.7e-6342.64Probable protein phosphatase 2C 77 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Match NameE-valueIdentityDescription
A0A6J1CZE11.3e-188100.00Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A6J1K7E62.1e-14680.66Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111491317... [more]
A0A6J1FLP99.9e-14481.90Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114469... [more]
A0A6J1JG011.3e-13075.07Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111484158... [more]
A0A5D3E3341.4e-12975.85Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT2G30020.19.0e-9754.50Protein phosphatase 2C family protein [more]
AT1G07160.13.3e-9155.49Protein phosphatase 2C family protein [more]
AT2G40180.19.0e-8955.43phosphatase 2C5 [more]
AT1G67820.17.2e-6243.81Protein phosphatase 2C family protein [more]
AT4G08260.15.3e-5748.97Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00331PP2C_SIG_2coord: 110..343
e-value: 0.0087
score: 1.4
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 82..341
e-value: 5.5E-82
score: 288.3
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 97..335
e-value: 4.5E-59
score: 200.2
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 91..343
score: 52.120689
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 91..343
e-value: 1.34334E-89
score: 267.27
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 92..346
e-value: 7.1E-85
score: 286.7
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 91..344
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR13832:SF759PROTEIN PHOSPHATASE 2C FAMILY PROTEIN-RELATEDcoord: 11..346
IPR015655Protein phosphatase 2C familyPANTHERPTHR13832PROTEIN PHOSPHATASE 2Ccoord: 11..346
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 123..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g32900.1Moc09g32900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity