Moc09g31400 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g31400
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr9: 23734365 .. 23734655 (-)
RNA-Seq ExpressionMoc09g31400
SyntenyMoc09g31400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGAAGATCAAAAAGCCGAGCAGGAGAAAACAAGGAAAGATATTGAGGAGTTGCGAGAAAAGTTAGATGCCATCCTCCTCGCGTTGGAAAAAGGCAAAGCGGCTGCAGATCCTGCTACATCTAGCAACCCTGTTCATGAGCCGCAAGAAACCCCGCCTTATCCACCTGGGTACGGTCCACCTCTTAGGCCAGTGGCAGAGGGTTTCATGCCACAATATACAACGTACAACCTTCTGTATGACGTCCCAGTTGGTCAGTATCCGCATCCCTTTGTTAAGGGAGCTTAG

mRNA sequence

ATGATGGAAGATCAAAAAGCCGAGCAGGAGAAAACAAGGAAAGATATTGAGGAGTTGCGAGAAAAGTTAGATGCCATCCTCCTCGCGTTGGAAAAAGGCAAAGCGGCTGCAGATCCTGCTACATCTAGCAACCCTGTTCATGAGCCGCAAGAAACCCCGCCTTATCCACCTGGGTACGGTCCACCTCTTAGGCCAGTGGCAGAGGGTTTCATGCCACAATATACAACGTACAACCTTCTGTATGACGTCCCAGTTGGTCAGTATCCGCATCCCTTTGTTAAGGGAGCTTAG

Coding sequence (CDS)

ATGATGGAAGATCAAAAAGCCGAGCAGGAGAAAACAAGGAAAGATATTGAGGAGTTGCGAGAAAAGTTAGATGCCATCCTCCTCGCGTTGGAAAAAGGCAAAGCGGCTGCAGATCCTGCTACATCTAGCAACCCTGTTCATGAGCCGCAAGAAACCCCGCCTTATCCACCTGGGTACGGTCCACCTCTTAGGCCAGTGGCAGAGGGTTTCATGCCACAATATACAACGTACAACCTTCTGTATGACGTCCCAGTTGGTCAGTATCCGCATCCCTTTGTTAAGGGAGCTTAG

Protein sequence

MMEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGYGPPLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKGA
Homology
BLAST of Moc09g31400 vs. NCBI nr
Match: XP_022157796.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia])

HSP 1 Score: 177.6 bits (449), Expect = 5.3e-41
Identity = 87/95 (91.58%), Postives = 89/95 (93.68%), Query Frame = 0

Query: 2  MEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGYGP 61
          MEDQKAEQEKTRKDIEELREKLD I L LEKGKA ADPATSSNP+HEPQETPPYPPGYGP
Sbjct: 1  MEDQKAEQEKTRKDIEELREKLDXIXLXLEKGKATADPATSSNPIHEPQETPPYPPGYGP 60

Query: 62 PLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKGA 97
          PLRPVAEGFMPQYTTYN LYDVP+GQYPH FVKGA
Sbjct: 61 PLRPVAEGFMPQYTTYNPLYDVPIGQYPHTFVKGA 95

BLAST of Moc09g31400 vs. NCBI nr
Match: XP_022149382.1 (uncharacterized protein LOC111017812 [Momordica charantia])

HSP 1 Score: 145.2 bits (365), Expect = 2.9e-31
Identity = 76/94 (80.85%), Postives = 79/94 (84.04%), Query Frame = 0

Query: 2  MEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGYGP 61
          M+DQKAEQEK RKDIEELREKLD ILLALEKGK  ADP TSSNPVH+ QE PPYP GY P
Sbjct: 1  MKDQKAEQEKIRKDIEELREKLDVILLALEKGKTVADPTTSSNPVHDQQEIPPYPLGYDP 60

Query: 62 PLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKG 96
          PLR VAEGFM Q TTYN LYD+ VGQYP PFVKG
Sbjct: 61 PLRLVAEGFMQQNTTYNPLYDILVGQYPPPFVKG 94

BLAST of Moc09g31400 vs. NCBI nr
Match: XP_022155098.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica charantia])

HSP 1 Score: 121.3 bits (303), Expect = 4.5e-24
Identity = 66/102 (64.71%), Postives = 78/102 (76.47%), Query Frame = 0

Query: 1  MMEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGY- 60
          M +D+K+EQEKTRKDIEELREKLDAILLALEKGK  A+   +SNP+HEP  TP +PPG+ 
Sbjct: 1  MEDDRKSEQEKTRKDIEELREKLDAILLALEKGKTIAE---TSNPIHEP-PTPQFPPGFE 60

Query: 61 ------GPPLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKG 96
                GP  RP+ EGFM QYTTYN LYD+P GQ+P P V+G
Sbjct: 61 PSFRPAGPSFRPMTEGFMSQYTTYNPLYDIPAGQFPPPLVRG 98

BLAST of Moc09g31400 vs. NCBI nr
Match: XP_022147190.1 (uncharacterized protein LOC111016201 [Momordica charantia])

HSP 1 Score: 103.6 bits (257), Expect = 9.7e-19
Identity = 54/56 (96.43%), Postives = 55/56 (98.21%), Query Frame = 0

Query: 1   MMEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYP 57
           MMEDQKAEQEKTRKDIEELREKLD ILLALEKGK+AADPATSSNPVHEPQETPPYP
Sbjct: 481 MMEDQKAEQEKTRKDIEELREKLDVILLALEKGKSAADPATSSNPVHEPQETPPYP 536

BLAST of Moc09g31400 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 55.5 bits (132), Expect = 3.0e-04
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = 0

Query: 71 MPQYTTYNLLYDVPVGQYPHPFVKGA 97
          MPQYTTYN LYDVPVGQY HPFVKGA
Sbjct: 1  MPQYTTYNPLYDVPVGQYLHPFVKGA 26

BLAST of Moc09g31400 vs. ExPASy TrEMBL
Match: A0A6J1DZ90 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.6e-41
Identity = 87/95 (91.58%), Postives = 89/95 (93.68%), Query Frame = 0

Query: 2  MEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGYGP 61
          MEDQKAEQEKTRKDIEELREKLD I L LEKGKA ADPATSSNP+HEPQETPPYPPGYGP
Sbjct: 1  MEDQKAEQEKTRKDIEELREKLDXIXLXLEKGKATADPATSSNPIHEPQETPPYPPGYGP 60

Query: 62 PLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKGA 97
          PLRPVAEGFMPQYTTYN LYDVP+GQYPH FVKGA
Sbjct: 61 PLRPVAEGFMPQYTTYNPLYDVPIGQYPHTFVKGA 95

BLAST of Moc09g31400 vs. ExPASy TrEMBL
Match: A0A6J1D7T3 (uncharacterized protein LOC111017812 OS=Momordica charantia OX=3673 GN=LOC111017812 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-31
Identity = 76/94 (80.85%), Postives = 79/94 (84.04%), Query Frame = 0

Query: 2  MEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGYGP 61
          M+DQKAEQEK RKDIEELREKLD ILLALEKGK  ADP TSSNPVH+ QE PPYP GY P
Sbjct: 1  MKDQKAEQEKIRKDIEELREKLDVILLALEKGKTVADPTTSSNPVHDQQEIPPYPLGYDP 60

Query: 62 PLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKG 96
          PLR VAEGFM Q TTYN LYD+ VGQYP PFVKG
Sbjct: 61 PLRLVAEGFMQQNTTYNPLYDILVGQYPPPFVKG 94

BLAST of Moc09g31400 vs. ExPASy TrEMBL
Match: A0A6J1DM29 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia OX=3673 GN=LOC111022231 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.2e-24
Identity = 66/102 (64.71%), Postives = 78/102 (76.47%), Query Frame = 0

Query: 1  MMEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYPPGY- 60
          M +D+K+EQEKTRKDIEELREKLDAILLALEKGK  A+   +SNP+HEP  TP +PPG+ 
Sbjct: 1  MEDDRKSEQEKTRKDIEELREKLDAILLALEKGKTIAE---TSNPIHEP-PTPQFPPGFE 60

Query: 61 ------GPPLRPVAEGFMPQYTTYNLLYDVPVGQYPHPFVKG 96
                GP  RP+ EGFM QYTTYN LYD+P GQ+P P V+G
Sbjct: 61 PSFRPAGPSFRPMTEGFMSQYTTYNPLYDIPAGQFPPPLVRG 98

BLAST of Moc09g31400 vs. ExPASy TrEMBL
Match: A0A6J1CZG4 (uncharacterized protein LOC111016201 OS=Momordica charantia OX=3673 GN=LOC111016201 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.7e-19
Identity = 54/56 (96.43%), Postives = 55/56 (98.21%), Query Frame = 0

Query: 1   MMEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAADPATSSNPVHEPQETPPYP 57
           MMEDQKAEQEKTRKDIEELREKLD ILLALEKGK+AADPATSSNPVHEPQETPPYP
Sbjct: 481 MMEDQKAEQEKTRKDIEELREKLDVILLALEKGKSAADPATSSNPVHEPQETPPYP 536

BLAST of Moc09g31400 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.5e-04
Identity = 24/26 (92.31%), Postives = 24/26 (92.31%), Query Frame = 0

Query: 71 MPQYTTYNLLYDVPVGQYPHPFVKGA 97
          MPQYTTYN LYDVPVGQY HPFVKGA
Sbjct: 1  MPQYTTYNPLYDVPVGQYLHPFVKGA 26

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157796.15.3e-4191.58LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia][more]
XP_022149382.12.9e-3180.85uncharacterized protein LOC111017812 [Momordica charantia][more]
XP_022155098.14.5e-2464.71LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica ch... [more]
XP_022147190.19.7e-1996.43uncharacterized protein LOC111016201 [Momordica charantia][more]
XP_022147189.13.0e-0492.31LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DZ902.6e-4191.58Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1[more]
A0A6J1D7T31.4e-3180.85uncharacterized protein LOC111017812 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1DM292.2e-2464.71LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia... [more]
A0A6J1CZG44.7e-1996.43uncharacterized protein LOC111016201 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A6J1D0991.5e-0492.31Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 2..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..63

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g31400.1Moc09g31400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0030430 host cell cytoplasm
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity