Moc09g30540 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g30540
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr9: 22978342 .. 22979146 (-)
RNA-Seq ExpressionMoc09g30540
SyntenyMoc09g30540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTCAACCAGCAAACTCGACCAATACGGCAGATCGAAGGACTCTAGCTGCCAGTGATTTCCACCAGAGGGAGGTCGGAGTCAGTGGTGTAGAGGGGCAAGGTCACGACGGCCTAGCAACAGAACCCCCTCCGCAAGTCGGCACAAATCACCGCGCCTGTCCTACCACCTGCGCACCCCCAAGGACATTCAAGGCCACCCGTGGCCGAGGTGGAACCTCTAAGAAGGGCGCCCGGGGTCTAGCCCCGGCCCCGACAAGTGAGAACTTGGACGCACTCCAGAGAGAAATGGAGGTATGCGCACGAAAATGCGGTCCATGGAGGAAATGTATAACGAAATGATATTGCTGCAGGCGCAGGGTCCCGATCTGAGAACCGAGTGACGCGCGTTGGCATACGCGAGCAAAGGGGGTTCCCACCTCGGCCCAGTCGAGGAGGAACATCCCGAAGGACAACGAGAGAGCGAGGACACACTCGCCAGAGAGGAGACCTCCGTGAACACCTCAACAGAAACAGAGGCTCATCTCTCCGAAAGGACAGTCACCATCATCCGCTCACACAGGAGCTCCAACCAGCAGGCTGAATCCTCTCGCAACCAGTAACTCCTGCAGGAGTGATTACAAGGGAGGAGTCCGACCAGCTGAGGGGCCAGCTCGACGCTCAGGTGGAGGCCTTAAAGGCCAAATGTGAGCAGAAGGAAGGTCCACTGAACGATGGCGACTTGGGAGAATCGCCCTTCACCTCGGACGTTTTGGAAGCACCGATCTCTCCGAAGTTCAAAGCTCCTACCCGTGAAACCTTATGA

mRNA sequence

ATGGTTCAACCAGCAAACTCGACCAATACGGCAGATCGAAGGACTCTAGCTGCCAGTGATTTCCACCAGAGGGAGGTCGGAGTCAGTGGTGTAGAGGGGCAAGGTCACGACGGCCTAGCAACAGAACCCCCTCCGCAAGTCGGCACAAATCACCGCGCCTGTCCTACCACCTGCGCACCCCCAAGGACATTCAAGGCCACCCGTGGCCGAGGTGGAACCTCTAAGAAGGGCGCCCGGGGTCTAGCCCCGGCCCCGACAAGCGCAGGGTCCCGATCTGAGAACCGAGTGACGCGCGTTGGCATACGCGAGCAAAGGGGGTTCCCACCTCGGCCCAGTCGAGGAGGAACATCCCGAAGGACAACGAGAGAGCGAGGACACACTCGCCAGAGAGGAGACCTCCGTGAACACCTCAACAGAAACAGAGGCTCATCTCTCCGAAAGGACAGAGTGATTACAAGGGAGGAGTCCGACCAGCTGAGGGGCCAGCTCGACGCTCAGGTGGAGGCCTTAAAGGCCAAATGTGAGCAGAAGGAAGGTCCACTGAACGATGGCGACTTGGGAGAATCGCCCTTCACCTCGGACGTTTTGGAAGCACCGATCTCTCCGAAGTTCAAAGCTCCTACCCGTGAAACCTTATGA

Coding sequence (CDS)

ATGGTTCAACCAGCAAACTCGACCAATACGGCAGATCGAAGGACTCTAGCTGCCAGTGATTTCCACCAGAGGGAGGTCGGAGTCAGTGGTGTAGAGGGGCAAGGTCACGACGGCCTAGCAACAGAACCCCCTCCGCAAGTCGGCACAAATCACCGCGCCTGTCCTACCACCTGCGCACCCCCAAGGACATTCAAGGCCACCCGTGGCCGAGGTGGAACCTCTAAGAAGGGCGCCCGGGGTCTAGCCCCGGCCCCGACAAGCGCAGGGTCCCGATCTGAGAACCGAGTGACGCGCGTTGGCATACGCGAGCAAAGGGGGTTCCCACCTCGGCCCAGTCGAGGAGGAACATCCCGAAGGACAACGAGAGAGCGAGGACACACTCGCCAGAGAGGAGACCTCCGTGAACACCTCAACAGAAACAGAGGCTCATCTCTCCGAAAGGACAGAGTGATTACAAGGGAGGAGTCCGACCAGCTGAGGGGCCAGCTCGACGCTCAGGTGGAGGCCTTAAAGGCCAAATGTGAGCAGAAGGAAGGTCCACTGAACGATGGCGACTTGGGAGAATCGCCCTTCACCTCGGACGTTTTGGAAGCACCGATCTCTCCGAAGTTCAAAGCTCCTACCCGTGAAACCTTATGA

Protein sequence

MVQPANSTNTADRRTLAASDFHQREVGVSGVEGQGHDGLATEPPPQVGTNHRACPTTCAPPRTFKATRGRGGTSKKGARGLAPAPTSAGSRSENRVTRVGIREQRGFPPRPSRGGTSRRTTRERGHTRQRGDLREHLNRNRGSSLRKDRVITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPTRETL
Homology
BLAST of Moc09g30540 vs. NCBI nr
Match: XP_022159327.1 (uncharacterized protein LOC111025738 [Momordica charantia])

HSP 1 Score: 153.7 bits (387), Expect = 1.8e-33
Identity = 110/239 (46.03%), Postives = 134/239 (56.07%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDFHQREVGVSGVEGQGHDGLATEPPPQVGTNHRACPTTCAP 60
           MVQP +STNT DRR L A+D HQREVG   VEGQ H+GL TEP      + R      +P
Sbjct: 1   MVQPVDSTNTGDRRALVANDGHQREVGAEVVEGQIHEGLGTEP---FCRSARITTPDLSP 60

Query: 61  --PRTFKATRGRGGTSKKGARGLAPAPT-----SAGSRSENRVTRVGIREQRGFPPRPSR 120
             P+ FKA RGRGG S++   G APAP+     +     E   T++   E+       + 
Sbjct: 61  AHPKPFKANRGRGGASRRTTLGAAPAPSRENFDALQKEMEAMRTQMLTMEEMYNEMVQAV 120

Query: 121 GGTSRRTTRERGHTRQRGDLREHLNRNRGSSLRKDR------------------------ 180
           G  SR  + +R    +RGDLR+HL+R R SSLRK R                        
Sbjct: 121 GAGSR--SEDRAARDERGDLRDHLSRKRSSSLRKGRSPSCSHKNSNQQAESSYNPVVPEG 180

Query: 181 VITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPT 209
           VITREE DQL+ + DAQVE LKA+CE K    +DGDLGESPFTSD+LEA I  KFK PT
Sbjct: 181 VITREEFDQLKSKFDAQVETLKARCEVKGSTFDDGDLGESPFTSDILEALIPSKFKTPT 234

BLAST of Moc09g30540 vs. NCBI nr
Match: XP_022151719.1 (uncharacterized protein LOC111019634 [Momordica charantia])

HSP 1 Score: 116.7 bits (291), Expect = 2.4e-22
Identity = 78/145 (53.79%), Postives = 87/145 (60.00%), Query Frame = 0

Query: 88  AGSRSENRVTRVGIREQRGFPPRPSRGGTSRRTTRERGHTRQRGDLREHLNRNRGSSLRK 147
           A SRSENRV R  + EQRG    P + G       E  +T QRGDLREHLNR R SSLRK
Sbjct: 21  ARSRSENRVARNDMHEQRGHHLGPVKKGHPEGGEDEE-YTHQRGDLREHLNRKRSSSLRK 80

Query: 148 ------------------------DRVITREESDQLRGQLDAQVEALKAKCEQKEGPLND 207
                                   + VITREE DQL+ + DAQVEALKAKCE+KE   +D
Sbjct: 81  GQSPSCSHRNSNQQAESSYNPITPEGVITREEFDQLKSKFDAQVEALKAKCEKKESSFDD 140

Query: 208 GDLGESPFTSDVLEAPISPKFKAPT 209
           GDLGESPFTSD+LEA I  KFK PT
Sbjct: 141 GDLGESPFTSDILEALIPLKFKTPT 164

BLAST of Moc09g30540 vs. NCBI nr
Match: XP_022137317.1 (uncharacterized protein LOC111008813 [Momordica charantia])

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-21
Identity = 57/59 (96.61%), Postives = 57/59 (96.61%), Query Frame = 0

Query: 150 VITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPT 209
           VITREE DQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPI PKFKAPT
Sbjct: 16  VITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPIPPKFKAPT 74

BLAST of Moc09g30540 vs. NCBI nr
Match: XP_022141796.1 (uncharacterized protein LOC111012081 [Momordica charantia])

HSP 1 Score: 111.3 bits (277), Expect = 1.0e-20
Identity = 55/59 (93.22%), Postives = 56/59 (94.92%), Query Frame = 0

Query: 150 VITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPT 209
           +ITREE DQLRGQLDAQ EALKAKCEQKEGPLNDGDLGESPFTSDVLEAPI PKFKAPT
Sbjct: 1   MITREEFDQLRGQLDAQXEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPIPPKFKAPT 59

BLAST of Moc09g30540 vs. NCBI nr
Match: XP_022152854.1 (uncharacterized protein LOC111020479 [Momordica charantia])

HSP 1 Score: 109.4 bits (272), Expect = 3.9e-20
Identity = 83/208 (39.90%), Postives = 96/208 (46.15%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDFHQREVGVSGVEGQGHDGLATEPPPQVGTNHRACPTTCAP 60
           MVQPANSTNTADRR LAA+  HQREVG   VEGQGH+ L TEP              C  
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEP-------------LC-- 60

Query: 61  PRTFKATRGRGGTSKKGARGLAPAPTSAGSRSENRVTRVGIREQRGFPPRPSRGGTSRRT 120
                          + AR   P    A                    P+PS+  +S   
Sbjct: 61  ---------------RSARITTPVLPPA-------------------HPKPSKAESSYNP 120

Query: 121 TRERGHTRQRGDLREHLNRNRGSSLRKDRVITREESDQLRGQLDAQVEALKAKCEQKEGP 180
                                        VITREE DQL+ + DAQVEALKA+CE+KE  
Sbjct: 121 I-------------------------TPGVITREEFDQLKSKFDAQVEALKARCEKKESS 134

Query: 181 LNDGDLGESPFTSDVLEAPISPKFKAPT 209
            +DGDLGE  F+SD+LEA I PKFK PT
Sbjct: 181 FDDGDLGELSFSSDILEALIPPKFKTPT 134

BLAST of Moc09g30540 vs. ExPASy TrEMBL
Match: A0A6J1DZJ1 (uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025738 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 8.7e-34
Identity = 110/239 (46.03%), Postives = 134/239 (56.07%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDFHQREVGVSGVEGQGHDGLATEPPPQVGTNHRACPTTCAP 60
           MVQP +STNT DRR L A+D HQREVG   VEGQ H+GL TEP      + R      +P
Sbjct: 1   MVQPVDSTNTGDRRALVANDGHQREVGAEVVEGQIHEGLGTEP---FCRSARITTPDLSP 60

Query: 61  --PRTFKATRGRGGTSKKGARGLAPAPT-----SAGSRSENRVTRVGIREQRGFPPRPSR 120
             P+ FKA RGRGG S++   G APAP+     +     E   T++   E+       + 
Sbjct: 61  AHPKPFKANRGRGGASRRTTLGAAPAPSRENFDALQKEMEAMRTQMLTMEEMYNEMVQAV 120

Query: 121 GGTSRRTTRERGHTRQRGDLREHLNRNRGSSLRKDR------------------------ 180
           G  SR  + +R    +RGDLR+HL+R R SSLRK R                        
Sbjct: 121 GAGSR--SEDRAARDERGDLRDHLSRKRSSSLRKGRSPSCSHKNSNQQAESSYNPVVPEG 180

Query: 181 VITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPT 209
           VITREE DQL+ + DAQVE LKA+CE K    +DGDLGESPFTSD+LEA I  KFK PT
Sbjct: 181 VITREEFDQLKSKFDAQVETLKARCEVKGSTFDDGDLGESPFTSDILEALIPSKFKTPT 234

BLAST of Moc09g30540 vs. ExPASy TrEMBL
Match: A0A6J1DDW5 (uncharacterized protein LOC111019634 OS=Momordica charantia OX=3673 GN=LOC111019634 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.2e-22
Identity = 78/145 (53.79%), Postives = 87/145 (60.00%), Query Frame = 0

Query: 88  AGSRSENRVTRVGIREQRGFPPRPSRGGTSRRTTRERGHTRQRGDLREHLNRNRGSSLRK 147
           A SRSENRV R  + EQRG    P + G       E  +T QRGDLREHLNR R SSLRK
Sbjct: 21  ARSRSENRVARNDMHEQRGHHLGPVKKGHPEGGEDEE-YTHQRGDLREHLNRKRSSSLRK 80

Query: 148 ------------------------DRVITREESDQLRGQLDAQVEALKAKCEQKEGPLND 207
                                   + VITREE DQL+ + DAQVEALKAKCE+KE   +D
Sbjct: 81  GQSPSCSHRNSNQQAESSYNPITPEGVITREEFDQLKSKFDAQVEALKAKCEKKESSFDD 140

Query: 208 GDLGESPFTSDVLEAPISPKFKAPT 209
           GDLGESPFTSD+LEA I  KFK PT
Sbjct: 141 GDLGESPFTSDILEALIPLKFKTPT 164

BLAST of Moc09g30540 vs. ExPASy TrEMBL
Match: A0A6J1C7X5 (uncharacterized protein LOC111008813 OS=Momordica charantia OX=3673 GN=LOC111008813 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.9e-22
Identity = 57/59 (96.61%), Postives = 57/59 (96.61%), Query Frame = 0

Query: 150 VITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPT 209
           VITREE DQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPI PKFKAPT
Sbjct: 16  VITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPIPPKFKAPT 74

BLAST of Moc09g30540 vs. ExPASy TrEMBL
Match: A0A6J1CKB3 (uncharacterized protein LOC111012081 OS=Momordica charantia OX=3673 GN=LOC111012081 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.0e-21
Identity = 55/59 (93.22%), Postives = 56/59 (94.92%), Query Frame = 0

Query: 150 VITREESDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPISPKFKAPT 209
           +ITREE DQLRGQLDAQ EALKAKCEQKEGPLNDGDLGESPFTSDVLEAPI PKFKAPT
Sbjct: 1   MITREEFDQLRGQLDAQXEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPIPPKFKAPT 59

BLAST of Moc09g30540 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.9e-20
Identity = 83/208 (39.90%), Postives = 96/208 (46.15%), Query Frame = 0

Query: 1   MVQPANSTNTADRRTLAASDFHQREVGVSGVEGQGHDGLATEPPPQVGTNHRACPTTCAP 60
           MVQPANSTNTADRR LAA+  HQREVG   VEGQGH+ L TEP              C  
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEP-------------LC-- 60

Query: 61  PRTFKATRGRGGTSKKGARGLAPAPTSAGSRSENRVTRVGIREQRGFPPRPSRGGTSRRT 120
                          + AR   P    A                    P+PS+  +S   
Sbjct: 61  ---------------RSARITTPVLPPA-------------------HPKPSKAESSYNP 120

Query: 121 TRERGHTRQRGDLREHLNRNRGSSLRKDRVITREESDQLRGQLDAQVEALKAKCEQKEGP 180
                                        VITREE DQL+ + DAQVEALKA+CE+KE  
Sbjct: 121 I-------------------------TPGVITREEFDQLKSKFDAQVEALKARCEKKESS 134

Query: 181 LNDGDLGESPFTSDVLEAPISPKFKAPT 209
            +DGDLGE  F+SD+LEA I PKFK PT
Sbjct: 181 FDDGDLGELSFSSDILEALIPPKFKTPT 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159327.11.8e-3346.03uncharacterized protein LOC111025738 [Momordica charantia][more]
XP_022151719.12.4e-2253.79uncharacterized protein LOC111019634 [Momordica charantia][more]
XP_022137317.11.2e-2196.61uncharacterized protein LOC111008813 [Momordica charantia][more]
XP_022141796.11.0e-2093.22uncharacterized protein LOC111012081 [Momordica charantia][more]
XP_022152854.13.9e-2039.90uncharacterized protein LOC111020479 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DZJ18.7e-3446.03uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DDW51.2e-2253.79uncharacterized protein LOC111019634 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A6J1C7X55.9e-2296.61uncharacterized protein LOC111008813 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A6J1CKB35.0e-2193.22uncharacterized protein LOC111012081 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A6J1DHB31.9e-2039.90uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..212
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 116..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g30540.1Moc09g30540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090304 nucleic acid metabolic process
cellular_component GO:0016020 membrane