Moc09g10720 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g10720
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Reverse transcriptase domain-containing protein
Location	chr9: 9103396 .. 9107217 (-)
RNA-Seq Expression	Moc09g10720
Synteny	Moc09g10720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGTGTTTCTACGGACATCCTGAGGAAGAAAAGAGACAGCTCTCATGGGACCTTATGCGATGGCTGAAAGGGAGTGATTCAATCTCATGGCTGATAGGAAGAGACTTTAATGGCATATTAAGTCATGATGAGAAATGGGGGGGAAACCAGAAGAATGATAAGTTGATCAATGACTTCAGTCAGGCGATGGATGATTGCAATTTAATAGATTTAGGCTTCTCTGGTGAGCCGTTTACGTGGTGTAATAGAAGGCCAAATGGGAAGGCTGTATATGAATGATTGATCGGGTGTGTTTTAACCCAGCGTGGGCAAGTTTATTTCCAAATAGTATTAAACACCACTTGGAGTATAGGACATCCAATCATATACCACTGGCGCTAAACATAGGGGAAGAATTGGTATGGAAGGCAAAGAGGTCCGCAAGAATTTACAGATTTGAGGAAACCTGGCTTGAAGATATAAACTGCAAACATATAGTTAAGAATAGTTGGAGTAGTGGAAATTTTGATGGCAGCCCTCAGTCTCTGATCTCAAAGCTGAATTATTGTGCAACTCACCTATCCCATTGGGGATGAAATAAAGCTGGAAATTATCGTAGAAGACTGAAAGAAGCGGAAACCCAACTGCAAAAAGCAATTAAAAACCTCCCAAGCGTGGGAGACCGAACAGAAATTCTTATGGCAGAGATGAATATGGCAAAGCTTTTAAACGAGGAAGAAATATATTGGCGACAACGCTCAAGAGAGCAATGGCTTAAATGGGGGTATCAGAATACTAAGTGGTTCCATAATAAAGCATCTCATAGAAAGCGGAAGAACGAAATCAAGGGATTGTTTTACTCGTAAGGAAGATGGGAAGCGGACCAAAATAAAATCGCCGATATGATATCTAACTACTTCTCTGATTTATTTTCTTCTTCAGGTCCTAGGAGGCTGGACATTTTTCATGTGTCCCGTTGTATTGTGCCCCGAGTCACTTCGGCCATAAACTGAGAGCTTCTAAAGAGTTTCAGTGAAGATGAAATTCAAGAGGCTATGGGTCAAATCCATCCAAATAAATGGCCAGGTCCGGATGGTTTCTCTGGAGCTTTCTACAAAAATTTTTTAGCACATTGTGGGCAAGGATGTTATAGCCTGTTGTTTGAATGTTTTGAATAATGATTTGGATATGAGACCTCTAAATGAAACAATGCTAGTACTGATCCTAAAATGCAATAACCCTCAGCGAGTGACTGATTTTAGGCCAATCTCTCTGTGTAATGTTTGTTATAAGATCATCTCAAAGGTGTTGGTTAATAGGCTGAAGAAGGTGCTTAATGCGATCATTTCACCCAACCAAAGTGCATTTATTCCTAAAAGACATATCACTGACAATGCAATATTGGGATATGAATGCATTCATTCCCTTAGAAGCAAGAAAGGAGGAAAATCCGGTTGGGCGGCTTTGAAACTTGACATGAGTAAGGCCTATGACCGTGTTGAATGGGCGTTCCTGGAAGAGATAATGTTGAGGCTAGGGTTTGATAAGGCCTCAGTTGATTTAATTATGAGGTGTGTTCGATCAGTTACGTTTTCCTTCAAGCTCAATGGAGAGAAAGTTGGCCATGTCACTCCCCAAAGGGGACTCTGTCAGGGGGACTCATTATCGCCATACTTGTTTCTCATGTGCGCAGAAGGGTTGTCTAGCCTTCTCCATCAGTTCTCAATTGAGAAAAAAGTCTTTGGACTATCTATTGCTCGGCGAAGCCCTCCTATCTCTCACATCTTCTTCGCGGTGACAGCCTCTTATTTTTTAAGGCCAAAATTAGTGAAGGAGCTTGCATTGGTGAATGTCTTAAACGGTATGAAGTTGCTTCGGGGCAGGTTATAAATTTCGATAAATCCGTTCTTGCATTTAGTCCAAACACGAATGAAGCTTTTAAGGAACAACTAAGGGATCTTCTTTCTGTTAGAGTTGTGGCCTGCCATCACCAATATTTGGGTCTCCCTTCCTTTCTATCCCGGAACAAAACTATGCATTTCAATTACATTAAGGACCGTGTGTGGAAGAACCTTCAAGGCTGGAAAAGTAGACTGTTTTCAATGGGAGGTAAGGAGGTGTTAATTAAAGCAGTTATACAGGCTATTCCGTGCTACACGATGTCTTGTTTTAGACTGCCGAAGAAACTTATTCAGGAGCTTAATCAACTGGTGGCTCGTTTTTGGTGGGGTGTAGATGGAGAGGACAGGAAGATCCATTGGGTTTGCTGGAAATTAATGTGTAAACCGAAATGCTTGGGAGGAATGGGCTTTAGGGATCTTGAAATTTTCAACAAGGCTCTGCTAGCAAAACAAGGTTGGAGGATTTTAAATGACCTGAACTCAATGCTTGCACAAGTCCTAAAGGGACGTTATTTCAAATAGGGTACCTTTTTATCTACAAAACTCGGTTGGAATCCTTCGTTCATTTGGAGAAATATTCTGTGGGGTCGGGATTTACTTAAAGAGGGAACCCGATGGCGCATTGGGAATGGTGAAAGGGTGAAGATTTATGGTGATAATTGGGTTCCTAATCAACCAACTCTGAAAATTCTATCTCGGCCCCAAATTCCCATTGACACAAACGTTAGTCATCTGATTGATAATGAGCTAGGCCAGTGGAAGGCAGATATAGTTCAAGATATTTTCTCGCCAGATGAAGCTAAGGGTATTATATTGATTCCTATTGGGATGAGCCATGGATGGGATCGTTTGATTTGGCACCATGAGAAATTGGGTATATACACAGTTAAAAGTGGATATAGAGCTGCTCAGATGGCATTATCAAATAACTTGGCCTCATCCTCTTCTACGGATGGCTTGGCGAATTGGTGGGGAGGAGTGTGGAATTTATCTTTCCCTAGTAAAATAAAAGTCTTCTTTTGGCGTTTGTGTTTAGACAGGCTCCCAATGAGAGCAAATTTGACTCAAAGGGAGGTGGATGTCCAAAATATTTGTGTTTTCTGTGGGAAGAAAGGAGAAGATTCTTACCATTTATTTTGGCTTCGTAAATATACCAAACATAAATGGATGAATTCTAAATTTCACCATCTCTCTGTTCTTCATCCTCAGGCACCTATGATTAATATCCTAAGGGACTGGCGAGACATGCTAAATTGGGAAGATTTTGAGGAATTGGTCGTGTTTTTGTGGGGTTTATGGAATCGTAGAAATGCATTTGTGTTTAATAAGAGAAGAGCTGAGGATGATGATTTGGCAGGATGGGTCAGTACTTACATTGCTACATTCAGGGCTACTAATACCAATCATGCAACAGCCAATCAAGACTAGCTTTCAACAGCTTTCACAAATACATCAAGCTCAAAATCACACTACTTGGTGCCCACCAGAAGAGGGGATCTTTAAATTAAACACAGATGCATCTTTCTCCTCTATCGATTTTAATGCAGGTCTGGGAGTCATCATCAGAGACCATAGAGGGCAAGTTCTAGCTTCGGCTACGAAATACCTAGAGCATGTGGCGTCCGGGGATGATGCTGAAGCGCTTGCTGCAGTGGAAGGCTTTCGTGTGGCAATGGAGACTGGAATTTCTCCGATCCTTTTGGTAACTGACTCTTTGCGTATCTACAACCTTTTGCTCGAGATAAAGAAGTCCTATCAGAGACGAGATCAATTATTGAATATGCGAAAACTCATCTTGCTACTAGATTGTAGGTATCCTACAGCTTCACAAAAAGAGGTGGAAATACGATCGCTCACCTTTTGGCGAGAAGAGCCCTCCGGTCTCAGGAAAATTTCGTCTGGCTTGAGGAGGGGCCGGAGGAGATCTCAAACACTCTAG

mRNA sequence

ATGACGTGTTTCTACGGACATCCTGAGGAAGAAAAGAGACAGCTCTCATGGGACCTTATGCGATGGCTGAAAGGGAGTGATTCAATCTCATGGCTGATAGGAAGAGACTTTAATGGCATATTAAGTCATGATGAGAAATGGGGGGGAAACCAGAAGAATGATAAGTTGATCAATGACTTCAGTCAGGCGATGGATGATTGCAATTTAATAGATTTAGGCTTCTCTGAGGGAACCCGATGGCGCATTGGGAATGGTGAAAGGGTGAAGATTTATGGTGATAATTGGGTTCCTAATCAACCAACTCTGAAAATTCTATCTCGGCCCCAAATTCCCATTGACACAAACGTTAGTCATCTGATTGATAATGAGCTAGGCCAGTGGAAGGCAGATATAGTTCAAGATATTTTCTCGCCAGATGAAGCTAAGGGTATTATATTGATTCCTATTGGGATGAGCCATGGATGGGATCGTTTGATTTGGCACCATGAGAAATTGGGTATATACACAGTTAAAAGTGGATATAGAGCTGCTCAGATGGCATTATCAAATAACTTGGCCTCATCCTCTTCTACGGATGGCTTGGCGAATTGGTGGGGAGGAGTGTGGAATTTATCTTTCCCTAGTAAAATAAAAGTCTTCTTTTGGCGTTTGTGTTTAGACAGGCTCCCAATGAGAGCAAATTTGACTCAAAGGGAGGTGGATGTCCAAAATATTTGTGTTTTCTGTGGGAAGAAAGGAGAAGATTCTTACCATTTATTTTGGCTTCGTAAATATACCAAACATAAATGGATGAATTCTAAATTTCACCATCTCTCTGTTCTTCATCCTCAGGCACCTATGATTAATATCCTAAGGGACTGGCGAGACATGCTAAATTGGGAAGATTTTGAGGAATTGGTCGTGTTTTTGTGGGGTTTATGGAATCGTAGAAATGCATTTGTGTTTAATAAGAGAAGAGCTGAGGATGATGATTTGGCAGGATGGGTCAGTCTGGGAGTCATCATCAGAGACCATAGAGGGCAAGTTCTAGCTTCGGCTACGAAATACCTAGAGCATGTGGCGTCCGGGGATGATGCTGAAGCGCTTGCTGCAGTGGAAGGCTTTCGTGTGGCAATGGAGACTGGAATTTCTCCGATCCTTTTGGTAACTGACTCTTTGCGTATCTACAACCTTTTGCTCGAGATAAAGAAGTCCTATCAGAGACGAGATCAATTATTGAATATGCGAAAACTCATCTTGCTACTAGATTGTAGGTATCCTACAGCTTCACAAAAAGAGGTGGAAATACGATCGCTCACCTTTTGGCGAGAAGAGCCCTCCGGTCTCAGGAAAATTTCGTCTGGCTTGAGGAGGGGCCGGAGGAGATCTCAAACACTCTAG

Coding sequence (CDS)

ATGACGTGTTTCTACGGACATCCTGAGGAAGAAAAGAGACAGCTCTCATGGGACCTTATGCGATGGCTGAAAGGGAGTGATTCAATCTCATGGCTGATAGGAAGAGACTTTAATGGCATATTAAGTCATGATGAGAAATGGGGGGGAAACCAGAAGAATGATAAGTTGATCAATGACTTCAGTCAGGCGATGGATGATTGCAATTTAATAGATTTAGGCTTCTCTGAGGGAACCCGATGGCGCATTGGGAATGGTGAAAGGGTGAAGATTTATGGTGATAATTGGGTTCCTAATCAACCAACTCTGAAAATTCTATCTCGGCCCCAAATTCCCATTGACACAAACGTTAGTCATCTGATTGATAATGAGCTAGGCCAGTGGAAGGCAGATATAGTTCAAGATATTTTCTCGCCAGATGAAGCTAAGGGTATTATATTGATTCCTATTGGGATGAGCCATGGATGGGATCGTTTGATTTGGCACCATGAGAAATTGGGTATATACACAGTTAAAAGTGGATATAGAGCTGCTCAGATGGCATTATCAAATAACTTGGCCTCATCCTCTTCTACGGATGGCTTGGCGAATTGGTGGGGAGGAGTGTGGAATTTATCTTTCCCTAGTAAAATAAAAGTCTTCTTTTGGCGTTTGTGTTTAGACAGGCTCCCAATGAGAGCAAATTTGACTCAAAGGGAGGTGGATGTCCAAAATATTTGTGTTTTCTGTGGGAAGAAAGGAGAAGATTCTTACCATTTATTTTGGCTTCGTAAATATACCAAACATAAATGGATGAATTCTAAATTTCACCATCTCTCTGTTCTTCATCCTCAGGCACCTATGATTAATATCCTAAGGGACTGGCGAGACATGCTAAATTGGGAAGATTTTGAGGAATTGGTCGTGTTTTTGTGGGGTTTATGGAATCGTAGAAATGCATTTGTGTTTAATAAGAGAAGAGCTGAGGATGATGATTTGGCAGGATGGGTCAGTCTGGGAGTCATCATCAGAGACCATAGAGGGCAAGTTCTAGCTTCGGCTACGAAATACCTAGAGCATGTGGCGTCCGGGGATGATGCTGAAGCGCTTGCTGCAGTGGAAGGCTTTCGTGTGGCAATGGAGACTGGAATTTCTCCGATCCTTTTGGTAACTGACTCTTTGCGTATCTACAACCTTTTGCTCGAGATAAAGAAGTCCTATCAGAGACGAGATCAATTATTGAATATGCGAAAACTCATCTTGCTACTAGATTGTAGGTATCCTACAGCTTCACAAAAAGAGGTGGAAATACGATCGCTCACCTTTTGGCGAGAAGAGCCCTCCGGTCTCAGGAAAATTTCGTCTGGCTTGAGGAGGGGCCGGAGGAGATCTCAAACACTCTAG

Protein sequence

MTCFYGHPEEEKRQLSWDLMRWLKGSDSISWLIGRDFNGILSHDEKWGGNQKNDKLINDFSQAMDDCNLIDLGFSEGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQIPIDTNVSHLIDNELGQWKADIVQDIFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLASSSSTDGLANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLFWLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDFEELVVFLWGLWNRRNAFVFNKRRAEDDDLAGWVSLGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAMETGISPILLVTDSLRIYNLLLEIKKSYQRRDQLLNMRKLILLLDCRYPTASQKEVEIRSLTFWREEPSGLRKISSGLRRGRRRSQTL

Homology

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BLAST of Moc09g10720 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 322.4 bits (825), Expect = 6.3e-84
Identity = 170/363 (46.83%), Postives = 224/363 (61.71%), Query Frame = 0


Query: 76   EGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQIPIDTNVSHLIDNELGQWKADIVQDI 135
            +G RWRIGNG+ V IYGDNWVPNQPTLKILS P++P+ + VS L+D+E G W+ D+V+D 
Sbjct: 713  KGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDE 772

Query: 136  FSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNN----LASSSST 195
            F+PDEAKGI+ IPIG     DRLIW++EK G+Y+V+SGY+   +AL NN      SSSS+
Sbjct: 773  FTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYK---VALLNNPCVQAPSSSSS 832

Query: 196  DGLANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYH 255
            + +  WW G W +  P+KIKVF WRLCLDRLP   NL++R V++ N C FCG+ GEDS H
Sbjct: 833  EEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIH 892

Query: 256  LFWLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDFEELVVFLWGLWNRRN 315
            LFW+ K+ +  W+NSKF  LS      P + ILR+  + L+  DFEEL V +WGLWN+RN
Sbjct: 893  LFWICKFAEALWINSKFGKLS------PFL-ILRESHESLSKADFEELCVVIWGLWNQRN 952

Query: 316  AFVFNK-----------------------RRAEDDDLAGWVS------------------ 375
            A  FN                        R A+ + + G V+                  
Sbjct: 953  ARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAEILWQPPDEGIYKIN 1012

Query: 376  -------------LGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAMETGIS 381
                         LG+II + RGQV+A+ATKYLE++ S D AEA+AAVEG ++A E G+ 
Sbjct: 1013 TDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMH 1065

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BLAST of Moc09g10720 vs. NCBI nr
Match: XP_022143319.1 (uncharacterized protein LOC111013220 [Momordica charantia])

HSP 1 Score: 208.0 bits (528), Expect = 1.7e-49
Identity = 102/194 (52.58%), Postives = 128/194 (65.98%), Query Frame = 0


Query: 135 IFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLASSSSTDGL 194
           +F+ DE K I+ IP+G+    DRLIW+ EK GI TVKS Y+ A M   +  AS+S ++ L
Sbjct: 1   MFTYDEVKTILSIPLGIGLAADRLIWNFEKNGICTVKSDYKLAHMQSPDTSASTSLSECL 60

Query: 195 ANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLFW 254
           A WW  VW L+ PSKIKVFFWR CLDRLP  ANL  R VDV +   FCGKKGED+ HLFW
Sbjct: 61  AKWWKDVWQLNLPSKIKVFFWRPCLDRLPTGANLILRGVDVPDCYAFCGKKGEDALHLFW 120

Query: 255 LRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDFEELVVFLWGLWNRRNAFV 314
             K +K++   SKF HL        ++++LRD   +L+W DFEELVVFLWG+W++RN  V
Sbjct: 121 TCKVSKNQRQVSKFSHLPQDVRPLSLLHLLRDCEGILSWSDFEELVVFLWGIWSKRNVKV 180

Query: 315 FNKRRAEDDDLAGW 329
           F   R    DL GW
Sbjct: 181 FLNGREMVRDLDGW 194

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BLAST of Moc09g10720 vs. NCBI nr
Match: XP_023925698.1 (uncharacterized protein LOC112037116 [Quercus suber])

HSP 1 Score: 193.4 bits (490), Expect = 4.4e-45
Identity = 109/321 (33.96%), Postives = 164/321 (51.09%), Query Frame = 0


Query: 76  EGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQ-IPIDTNVSHLIDNELGQWKADIVQD 135
           +G+ WR+GNG  +++ GD W+PN PT K+L   Q +  D  V  LID     W  D +  
Sbjct: 470 QGSCWRVGNGASIRVLGDKWLPNHPTKKVLLPIQSVDNDLIVEELIDPVTRWWNRDFIMQ 529

Query: 136 IFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLASSSSTDGL 195
            F+ ++A+ I+ +P+   +  D L W   K G YTV+SGY+ A+  L      + S++G+
Sbjct: 530 NFNHEDAEAILRVPLSRRYISDSLFWTVNKSGEYTVRSGYQVAR-KLQKEADWAESSNGV 589

Query: 196 ANW--WGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHL 255
                W  +W L  P+KIKVF WR C + LP R NL QR V   N C  C  + E   H 
Sbjct: 590 VGGLVWRTLWKLKVPNKIKVFGWRACRNILPTRVNLVQRRVIQDNKCEACKIEAETGIHA 649

Query: 256 FWLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDFEELVVFLWGLWNRRNA 315
            W     +  W             QA M+ ++ +  + L+ E+ E+ +V  W +WN+RN 
Sbjct: 650 LWNCGVARDVWAGYTARVQKCSGDQADMLQLMEEMINRLSTEELEQFLVQSWIIWNQRNG 709

Query: 316 FVFNKR-RAEDDDLAGWVSLGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVA 375
            +  K+ +A ++D  G   +G IIR+ RG+V+AS +     V   +DAE LA       A
Sbjct: 710 LIHGKKLQAPEED--GTSGIGAIIRNDRGEVMASLSAKGPPVTCSEDAEILACRRAVNFA 769

Query: 376 METGISPILLVTDSLRIYNLL 393
           ME G S ++L  D+  I   L
Sbjct: 770 MECGFSELVLEGDNQAIMTAL 787

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BLAST of Moc09g10720 vs. NCBI nr
Match: XP_030969741.1 (uncharacterized protein LOC115990018 [Quercus lobata])

HSP 1 Score: 188.3 bits (477), Expect = 1.4e-43
Identity = 101/321 (31.46%), Postives = 159/321 (49.53%), Query Frame = 0


Query: 76  EGTRWRIGNGERVKIYGDNWVPNQPTLKILS-RPQIPIDTNVSHLIDNELGQWKADIVQD 135
           +G  W IG GE V+I  D W+P +    ++S  P +  D  VS LID +   WK + VQ 
Sbjct: 588 QGMVWHIGTGEAVRIKEDRWLPGRANCSVISPLPSLVPDVKVSTLIDQDTNAWKTEAVQQ 647

Query: 136 IFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLASSSSTDGL 195
           +F P EA+ I+ IP+      DR+IW H   G++T  S Y+      +++ A SS+ +  
Sbjct: 648 LFLPQEAEIILGIPLSTRRPVDRIIWAHTPSGMFTTCSAYKLLVSCDASSSAGSSNPEAQ 707

Query: 196 ANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLFW 255
             +W G+W L  P+KI+ F W +C + LP   NL +R++     C  C    EDS H  W
Sbjct: 708 KKFWKGIWQLRVPNKIRHFVWGICNNALPTMVNLHRRQIVPSASCALCNVLPEDSLHAVW 767

Query: 256 LRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDF--EELVVFLWGLWNRRNA 315
             +     W    + H +          +L  +  + N E+F  E  V+ +W LWNRRNA
Sbjct: 768 YCEAISGAWSTLDWFHQTAPPRPTSFTELLSSF--LCNKEEFRAEIFVIMVWLLWNRRNA 827

Query: 316 FVFNKRRAEDDDLAGWVSLGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAM 375
             F         +     +G+I R+H G+ + + +  +    S  D EALA ++  + A+
Sbjct: 828 IQFGHPPLPVASICSSAGIGIIARNHVGEAVGALSSPIPMAQSVADIEALACLKAVQFAL 887

Query: 376 ETGISPILLVTDSLRIYNLLL 394
           E G++ +++  DS  I N LL
Sbjct: 888 EIGLNRVVIEGDSAVIINALL 906

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BLAST of Moc09g10720 vs. NCBI nr
Match: KAF8408042.1 (hypothetical protein HHK36_007182 [Tetracentron sinense])

HSP 1 Score: 185.7 bits (470), Expect = 9.3e-43
Identity = 141/535 (26.36%), Postives = 224/535 (41.87%), Query Frame = 0


Query: 1   MTCFYGHPEEEKRQLSWDLMRWLKGSDSISWLIGRDFNGILSHDEKWGGNQKNDKLINDF 60
           +T  YGHPE  K+  +W+L+R+L  S S+ W+   DFN I   +EK G  +K    +  F
Sbjct: 103 LTGMYGHPEAAKKWETWELIRYLSRSYSMPWVCFGDFNEITCAEEKSGRVEKAAWKMRKF 162

Query: 61  SQAMDDCNLIDLGFSEGT-RW---RIGNG------------------------------- 120
            +A+ DC+LI LGF   T  W   R G G                               
Sbjct: 163 KEAILDCHLIGLGFEGNTFTWCNKRSGEGNVRERLDRAMATSDWCFLFPFTTVKHLSCHT 222

Query: 121 ------------ERVKI--------YGDNWVPNQPTLKIL----------SRPQIPIDTN 180
                       E  KI        +   W+ +    +I+          S   +P D  
Sbjct: 223 SDHSPLLLAFDKEAPKIARKKRSFRFEAMWIHSPECAEIIDSAWTGCHQVSAQVLPRDAK 282

Query: 181 VSHLIDNELGQWKADIVQDIFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYR 240
           VS LID +   W   ++  +F P EA+ I  IP+      D+ +WH    G ++V+S Y 
Sbjct: 283 VSLLIDKDQKTWNHTLLMTVFMPHEAELISSIPLSERLPPDKRVWHFTSKG-FSVRSAYH 342

Query: 241 AAQMALSNNLASSSSTDGLANW--------WGGVWNLSFPSKIKVFFWRLCLDRLPMRAN 300
                     A+SSST  L +W        W  VW L+ P K+K+F W++ L+ LP+RAN
Sbjct: 343 LTSTLRDRESATSSSTSSL-SWNGSLSGIKWSQVWQLAIPPKVKIFIWKVALNILPVRAN 402

Query: 301 LTQREVDVQNICVFCGKKGEDSYHLFWLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDW 360
           L +R++ V+N+C  CG++GE   H+     Y +  W+ S+      L   A   + L  W
Sbjct: 403 LCKRKIPVENVCGVCGEEGETILHVLKNCHYARQVWLLSQLG----LRSDATSADSLSSW 462

Query: 361 RDMLNWEDFEE----LVVFLWGLWNRRNAFVF------------------------NKRR 411
            + +     EE      +  W +W  RN ++F                        N R 
Sbjct: 463 VEEIMKSHGEEGLSAFFMIAWSIWKHRNEYIFSGVKMTPFNCVQRANKLLADFHNANDRA 522

[Close]

BLAST of Moc09g10720 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 3.1e-84
Identity = 170/363 (46.83%), Postives = 224/363 (61.71%), Query Frame = 0


Query: 76   EGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQIPIDTNVSHLIDNELGQWKADIVQDI 135
            +G RWRIGNG+ V IYGDNWVPNQPTLKILS P++P+ + VS L+D+E G W+ D+V+D 
Sbjct: 713  KGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDE 772

Query: 136  FSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNN----LASSSST 195
            F+PDEAKGI+ IPIG     DRLIW++EK G+Y+V+SGY+   +AL NN      SSSS+
Sbjct: 773  FTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYK---VALLNNPCVQAPSSSSS 832

Query: 196  DGLANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYH 255
            + +  WW G W +  P+KIKVF WRLCLDRLP   NL++R V++ N C FCG+ GEDS H
Sbjct: 833  EEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIH 892

Query: 256  LFWLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDFEELVVFLWGLWNRRN 315
            LFW+ K+ +  W+NSKF  LS      P + ILR+  + L+  DFEEL V +WGLWN+RN
Sbjct: 893  LFWICKFAEALWINSKFGKLS------PFL-ILRESHESLSKADFEELCVVIWGLWNQRN 952

Query: 316  AFVFNK-----------------------RRAEDDDLAGWVS------------------ 375
            A  FN                        R A+ + + G V+                  
Sbjct: 953  ARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITGRVTNTAEILWQPPDEGIYKIN 1012

Query: 376  -------------LGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAMETGIS 381
                         LG+II + RGQV+A+ATKYLE++ S D AEA+AAVEG ++A E G+ 
Sbjct: 1013 TDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMH 1065

[Close]

BLAST of Moc09g10720 vs. ExPASy TrEMBL
Match: A0A6J1CNZ5 (uncharacterized protein LOC111013220 OS=Momordica charantia OX=3673 GN=LOC111013220 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 8.4e-50
Identity = 102/194 (52.58%), Postives = 128/194 (65.98%), Query Frame = 0


Query: 135 IFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLASSSSTDGL 194
           +F+ DE K I+ IP+G+    DRLIW+ EK GI TVKS Y+ A M   +  AS+S ++ L
Sbjct: 1   MFTYDEVKTILSIPLGIGLAADRLIWNFEKNGICTVKSDYKLAHMQSPDTSASTSLSECL 60

Query: 195 ANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLFW 254
           A WW  VW L+ PSKIKVFFWR CLDRLP  ANL  R VDV +   FCGKKGED+ HLFW
Sbjct: 61  AKWWKDVWQLNLPSKIKVFFWRPCLDRLPTGANLILRGVDVPDCYAFCGKKGEDALHLFW 120

Query: 255 LRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNWEDFEELVVFLWGLWNRRNAFV 314
             K +K++   SKF HL        ++++LRD   +L+W DFEELVVFLWG+W++RN  V
Sbjct: 121 TCKVSKNQRQVSKFSHLPQDVRPLSLLHLLRDCEGILSWSDFEELVVFLWGIWSKRNVKV 180

Query: 315 FNKRRAEDDDLAGW 329
           F   R    DL GW
Sbjct: 181 FLNGREMVRDLDGW 194

[Close]

BLAST of Moc09g10720 vs. ExPASy TrEMBL
Match: A0A6J1CDQ4 (uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010533 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-42
Identity = 108/195 (55.38%), Postives = 113/195 (57.95%), Query Frame = 0


Query: 280 MINILRDWRDMLNWEDFEELVVFLWGLWNRRNAFVFNKRRAEDDDLAGWVS--------- 339
           MINILRDWRDMLNW+DFEELVVFLW LWNRRNAFVFNKRR EDDDLAGWVS         
Sbjct: 1   MINILRDWRDMLNWKDFEELVVFLWSLWNRRNAFVFNKRRVEDDDLAGWVSTYIATFKAT 60

Query: 340 ------------------------------------------------------LGV-II 399
                                                                 LGV II
Sbjct: 61  NTNHATANQDVSQSFQQSSQIHQAQNHTIWCPAEEGVFKLKTDASFSSIDFNAGLGVIII 120

Query: 400 RDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAMETGISPILLVTDSLRIYNLLLEI 411
           RDHRGQVLASATKYLEHVAS DDAEALAAVEG RVAMETGISPILL TDSLRIYNL    
Sbjct: 121 RDHRGQVLASATKYLEHVASVDDAEALAAVEGLRVAMETGISPILLETDSLRIYNLFARD 180

[Close]

BLAST of Moc09g10720 vs. ExPASy TrEMBL
Match: M5W5F3 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa026368mg PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.2e-42
Identity = 108/372 (29.03%), Postives = 171/372 (45.97%), Query Frame = 0


Query: 75  SEGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQIPIDTNVSHLIDNELGQWKADIVQD 134
           ++G RWR+G+G  +++Y D W+P     KI+S PQ+P+ T V  L  +  GQW   +++D
Sbjct: 622 NKGLRWRVGSGVSIQVYTDKWLPAPSCFKIMSPPQLPLSTRVCDLFTSS-GQWNVPLLKD 681

Query: 135 IFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLAS-SSSTDG 194
           IF   E   I+ IP+    G D LIWH+E+ G+Y+VKSGYR A +         S+  D 
Sbjct: 682 IFWDQEVDAILQIPLASLAGHDCLIWHYERNGMYSVKSGYRLAGLEKDKMSGEPSARVDL 741

Query: 195 LANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLF 254
            + +W  +W L  P+KIK F WR   D LP    L  R++    IC  C +K E   H  
Sbjct: 742 NSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQILFNRKIAPTPICPKCHRKAESVLHAV 801

Query: 255 WLRKYTKHKWMNSKFHHLSVLHPQAPMINILRD-WRDMLNWEDFEELVVF---LWGLWNR 314
           WL +  K  W NS + ++     +   +N  R+ W  +      EE  +F    WGLWNR
Sbjct: 802 WLCEAAKEVWRNSAWGNVC----EVWRVNSFRELWHALQLSSSGEEQGLFAYLCWGLWNR 861

Query: 315 RNAFVF--------------------------------NKRRAEDDDLAGW--------- 374
           RN+F+F                                 ++ +    L GW         
Sbjct: 862 RNSFIFEGKSETAIQLLSRMTKLAQEFSDANNILHTIHGRQSSPQAPLQGWRPPPAVKSG 921

Query: 375 ---VSLGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAMETGISPILLVTDS 398
                +GV++R+  G+ +A+  + +         E +A +EG R A++ G +  +L  D+
Sbjct: 922 DSVRGVGVVVRNANGEFMAACVRRIHASYGARQTELMATIEGLRFAIDMGFTDAILEMDA 981

[Close]

BLAST of Moc09g10720 vs. ExPASy TrEMBL
Match: A0A251NPF0 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G123900 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.2e-42
Identity = 108/372 (29.03%), Postives = 171/372 (45.97%), Query Frame = 0


Query: 75  SEGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQIPIDTNVSHLIDNELGQWKADIVQD 134
           ++G RWR+G+G  +++Y D W+P     KI+S PQ+P+ T V  L  +  GQW   +++D
Sbjct: 585 NKGLRWRVGSGVSIQVYTDKWLPAPSCFKIMSPPQLPLSTRVCDLFTSS-GQWNVPLLKD 644

Query: 135 IFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLAS-SSSTDG 194
           IF   E   I+ IP+    G D LIWH+E+ G+Y+VKSGYR A +         S+  D 
Sbjct: 645 IFWDQEVDAILQIPLASLAGHDCLIWHYERNGMYSVKSGYRLAGLEKDKMSGEPSARVDL 704

Query: 195 LANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLF 254
            + +W  +W L  P+KIK F WR   D LP    L  R++    IC  C +K E   H  
Sbjct: 705 NSKFWKKIWALKIPNKIKFFLWRCAWDFLPCGQILFNRKIAPTPICPKCHRKAESVLHAV 764

Query: 255 WLRKYTKHKWMNSKFHHLSVLHPQAPMINILRD-WRDMLNWEDFEELVVF---LWGLWNR 314
           WL +  K  W NS + ++     +   +N  R+ W  +      EE  +F    WGLWNR
Sbjct: 765 WLCEAAKEVWRNSAWGNVC----EVWRVNSFRELWHALQLSSSGEEQGLFAYLCWGLWNR 824

Query: 315 RNAFVF--------------------------------NKRRAEDDDLAGW--------- 374
           RN+F+F                                 ++ +    L GW         
Sbjct: 825 RNSFIFEGKSETAIQLLSRMTKLAQEFSDANNILHTIHGRQSSPQAPLQGWRPPPAVKSG 884

Query: 375 ---VSLGVIIRDHRGQVLASATKYLEHVASGDDAEALAAVEGFRVAMETGISPILLVTDS 398
                +GV++R+  G+ +A+  + +         E +A +EG R A++ G +  +L  D+
Sbjct: 885 DSVRGVGVVVRNANGEFMAACVRRIHASYGARQTELMATIEGLRFAIDMGFTDAILEMDA 944

[Close]

BLAST of Moc09g10720 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 8.4e-18
Identity = 64/254 (25.20%), Postives = 110/254 (43.31%), Query Frame = 0


Query: 76  EGTRWRIGNGERVKIYGDNWVPNQPTLKILSRPQIPIDTNVSHLIDNELGQ--WKADIVQ 135
           +GTR  IG+G+ ++I  DN V + P  + L+  +   +  +++L + +     W    + 
Sbjct: 36  KGTRHLIGDGQNIRIGLDNIVDSHPP-RPLNTEETYKEMTINNLFERKGSYYFWDDSKIS 95

Query: 136 DIFSPDEAKGIILIPIGMSHGWDRLIWHHEKLGIYTVKSGYRAAQMALSNNLASSSSTDG 195
                 +   I  I +  S   D++IW++   G YTV+SGY       S N+ + +   G
Sbjct: 96  QFVDQSDHGFIHRIYLAKSKKPDKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHG 155

Query: 196 LANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLF 255
             +    +WNL    K+K F WR     L     LT R + +   C  C ++ E   H  
Sbjct: 156 SIDLKTRIWNLPIMPKLKHFLWRALSQALATTERLTTRGMRIDPSCPRCHRENESINHAL 215

Query: 256 WLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNW------EDFEEL--VVFLWG 315
           +   +    W   +    S++  Q    +   +  ++LN+       DF +L  V  +W 
Sbjct: 216 FTCPFATMAW---RLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVWLIWR 275

Query: 316 LWNRRNAFVFNKRR 320
           +W  RN  VFNK R
Sbjct: 276 IWKARNNVVFNKFR 285

[Close]

BLAST of Moc09g10720 vs. TAIR 10
Match: AT1G33710.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.3e-05
Identity = 33/134 (24.63%), Postives = 53/134 (39.55%), Query Frame = 0


Query: 194 LANWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFCGKKGEDSYHLF 253
           + +W   VW      K     W   LDRLP +  L    + +Q  C  C    ED  HLF
Sbjct: 45  VVSWAKTVWFKGATPKHAFHMWVTNLDRLPTKTRLASWGMQLQTTCGLCSLDIEDRDHLF 104

Query: 254 WLRKYTKHKWMNSKFHHLSVLHPQAPMINILRDWRDMLNW---------EDFEELVV--F 313
              ++    W      H   +  + P  + +  W D+++W             +L+V   
Sbjct: 105 LTCEFACFLW------HTVSVRLELPAFSFV-VWNDLMDWTLQRNRRSPPTLRKLIVQSV 164

Query: 314 LWGLWNRRNAFVFN 317
           L+ +W +RN F+ N
Sbjct: 165 LYAIWKQRNNFLHN 171

[Close]

BLAST of Moc09g10720 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 16/47 (34.04%), Postives = 25/47 (53.19%), Query Frame = 0


Query: 196 NWWGGVWNLSFPSKIKVFFWRLCLDRLPMRANLTQREVDVQNICVFC 243
           NW G +W+L    KIK+  W+   + LP+ A L  R + ++  C  C
Sbjct: 4   NWIGDIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRC 50

The following BLAST results are available for this feature:

BLAST of Moc09g10720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150918.1	6.3e-84	46.83	uncharacterized protein LOC111018954 [Momordica charantia]	[more]
XP_022143319.1	1.7e-49	52.58	uncharacterized protein LOC111013220 [Momordica charantia]	[more]
XP_023925698.1	4.4e-45	33.96	uncharacterized protein LOC112037116 [Quercus suber]	[more]
XP_030969741.1	1.4e-43	31.46	uncharacterized protein LOC115990018 [Quercus lobata]	[more]
KAF8408042.1	9.3e-43	26.36	hypothetical protein HHK36_007182 [Tetracentron sinense]	[more]

BLAST of Moc09g10720 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc09g10720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAR4	3.1e-84	46.83	uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1CNZ5	8.4e-50	52.58	uncharacterized protein LOC111013220 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1CDQ4	1.3e-42	55.38	uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
M5W5F3	2.2e-42	29.03	Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=...	[more]
A0A251NPF0	2.2e-42	29.03	Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRU...	[more]

BLAST of Moc09g10720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09510.1	8.4e-18	25.20	Ribonuclease H-like superfamily protein	[more]
AT1G33710.1	1.3e-05	24.63	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT3G26855.1	1.4e-04	34.04	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 168..263 e-value: 2.5E-15 score: 57.0
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 330..405 e-value: 7.9E-11 score: 41.9
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 323..429 e-value: 4.0E-5 score: 25.6
None	No IPR available	PANTHER	PTHR46736	FAMILY NOT NAMED	coord: 62..387
None	No IPR available	PANTHER	PTHR46736:SF6	SUBFAMILY NOT NAMED	coord: 62..387
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 327..404

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g10720.1	Moc09g10720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity