Moc09g10030 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g10030
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUlp1-like peptidase
Locationchr9: 8499460 .. 8500924 (-)
RNA-Seq ExpressionMoc09g10030
SyntenyMoc09g10030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCAGGGTTTTGACAACGACGAGGATGTTGTCAAGGTCGCCATAGTTTACTTCATAGAACTTGCCATGATGAGTAAGGAGAGGAAGCAGTACATGGATACGAACCTGTTAGGTATTGTGGATCGGTGGGAGTTGTTTTGCAACTATGACTAGAGTATGATGATTTTCGAAAGTACAATTTGGAGCCTGAAGAACACTCTAAAGGATAAACTACAAGCGTACCAACAAAAGGCGAGAGACGACCCTTGACAGTTGAGACTTATAGTCTCTATGGGTTCGTGTACGCGATCCGGGTTAGTGAGGTTTAACGGTATAATTAGACTCATTATTTAACTACATCATTTATTTGACTTCTTGTGATTCCAAGTATGGGCACACGAGACTATCTCTACATTAAGTGTGCGAGTAGCAATGAGGTTGAGTGACGATGCCATTTCTTGACTTCTTAGATGGTCGTGAACCTATTCTCGCTGGTTTTGTGTGTTGGCGAGTGATGTATTCGATAACATGCAAGTAACTAATTCTCAAATGATCGTATGCTTGACATTTGTTCTTTCATTTGGAAATGTACACACCAATCGGAAAGTATGGGAGGAAAGTGGTAAGTTACAACTCAACATGTGCCATTCCTCCACAGTTGGACGAACAGTTCCAGAAATGGTTGGATGACCCATTAACGAACGGGGTAACGTTGTCTACTGCAGCTGGAATGCAAACGAAGGAATGGTATCACAATCTACTAGACCAGACTATCGAATTGAAGGATGAAGTAAGGACTTCTTACTCCCTAAGTATGACGTTGTGAATATGTTAGTTACATCATTGTGAATATATTAAATGAATCAGGTTATCGATGCGCTGGTGTTGTTTACGACGAAGAAGTTGGAGAAGTGTCTCCATCTATGTCGCAAGAGGTTTGCCATAGGCGACATACTTCTCTCTGTAAGTAATTTTTACCTTAATATGCCCACATAATAAAGTTTTTGTTCTAATTATTCATGAAAGTCTTATGTAACGTATTTATGCACAGACTTTGCTAAACCGCACAAACGGTCTATACGCTGCCATGAAGCCGGGAGTCATGGCCTCGAGGATCAAGTATGAATGGAGTAACGAGAAAACCATCTTCCGATACGTGTTTGGTAGACAATAGAACCACAACACGCCATGGAGTGATGCAGACATGGTATATACCACGATTAACATTGGTGAGAACCATTAGGTGATGCTCGGCATCGATCTTGTAGAGGGCGACATAACTGTATGGGATTCGCTACAATCTGCCACCCCACTAGAGGCACTCGAGAAGGAGCTCAAGCCGATGTGTATGATCCTCCCAATGGTGCTTCACTACAGCGGCATTTTTGCAGTACGACCCAACTTGCCAGTTATGCCATGGAGGGTGCGTCGGGTTCGCACACCCCAACAAAGTAAAGCGACGAATTGTGGTGAAGGGATGTGA

mRNA sequence

ATGGAGCAGGGTTTTGACAACGACGAGGATGTTGTCAAGGTCGCCATAGTTTACTTCATAGAACTTGCCATGATGAGTAAGGAGAGGAAGCAGTACATGGATACGAACCTGTTAGCTGGAATGCAAACGAAGGAATGGTATCACAATCTACTAGACCAGACTATCGAATTGAAGGATGAAGTTATCGATGCGCTGGTGTTGTTTACGACGAAGAAGTTGGAGAAGTGTCTCCATCTATGTCGCAAGAGGTTTGCCATAGGCGACATACTTCTCTCTGTAAACTTTGCTAAACCGCACAAACGGTCTATACGCTGCCATGAAGCCGGGAGTCATGGCCTCGAGGATCAAGTGATGCTCGGCATCGATCTTGTAGAGGGCGACATAACTGTATGGGATTCGCTACAATCTGCCACCCCACTAGAGGCACTCGAGAAGGAGCTCAAGCCGATGTGTATGATCCTCCCAATGGTGCTTCACTACAGCGGCATTTTTGCAGTACGACCCAACTTGCCAGTTATGCCATGGAGGGTGCGTCGGGTTCGCACACCCCAACAAAGTAAAGCGACGAATTGTGGTGAAGGGATGTGA

Coding sequence (CDS)

ATGGAGCAGGGTTTTGACAACGACGAGGATGTTGTCAAGGTCGCCATAGTTTACTTCATAGAACTTGCCATGATGAGTAAGGAGAGGAAGCAGTACATGGATACGAACCTGTTAGCTGGAATGCAAACGAAGGAATGGTATCACAATCTACTAGACCAGACTATCGAATTGAAGGATGAAGTTATCGATGCGCTGGTGTTGTTTACGACGAAGAAGTTGGAGAAGTGTCTCCATCTATGTCGCAAGAGGTTTGCCATAGGCGACATACTTCTCTCTGTAAACTTTGCTAAACCGCACAAACGGTCTATACGCTGCCATGAAGCCGGGAGTCATGGCCTCGAGGATCAAGTGATGCTCGGCATCGATCTTGTAGAGGGCGACATAACTGTATGGGATTCGCTACAATCTGCCACCCCACTAGAGGCACTCGAGAAGGAGCTCAAGCCGATGTGTATGATCCTCCCAATGGTGCTTCACTACAGCGGCATTTTTGCAGTACGACCCAACTTGCCAGTTATGCCATGGAGGGTGCGTCGGGTTCGCACACCCCAACAAAGTAAAGCGACGAATTGTGGTGAAGGGATGTGA

Protein sequence

MEQGFDNDEDVVKVAIVYFIELAMMSKERKQYMDTNLLAGMQTKEWYHNLLDQTIELKDEVIDALVLFTTKKLEKCLHLCRKRFAIGDILLSVNFAKPHKRSIRCHEAGSHGLEDQVMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWRVRRVRTPQQSKATNCGEGM
Homology
BLAST of Moc09g10030 vs. NCBI nr
Match: XP_022158807.1 (uncharacterized protein LOC111025273 [Momordica charantia])

HSP 1 Score: 155.2 bits (391), Expect = 5.7e-34
Identity = 85/192 (44.27%), Postives = 111/192 (57.81%), Query Frame = 0

Query: 40  GMQTKEWYHNLLDQTIELKDEVIDALVLFTTKKLEKCLHLCRKRFAIGDILLS------- 99
           G++ K W+  LLD   +L DE ID+L++ T +K+EKC HL R +FAIGD+LLS       
Sbjct: 16  GIKYKSWFGLLLDPQFQLDDEYIDSLMMLTARKVEKCKHLLRVQFAIGDVLLSNLLRRTD 75

Query: 100 --------------VNFAKPHKRSIRCHEAGSH-------------------GLEDQVML 159
                           +    +R+I  +  G                     G    VM+
Sbjct: 76  GPYAAMKPGVLPSKCTYDWRQERTIFRYVLGRQSDYDTLWSEADIVYTRMNIGGNHWVMI 135

Query: 160 GIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWRVRR 192
           GIDLVEGD+TVWDSLQ+ TPLE LEK LKPMC I+P +LH+SGI A+RPNLP++PWRVRR
Sbjct: 136 GIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGILALRPNLPMVPWRVRR 195

BLAST of Moc09g10030 vs. NCBI nr
Match: XP_022148137.1 (uncharacterized protein LOC111016890 [Momordica charantia])

HSP 1 Score: 132.1 bits (331), Expect = 5.2e-27
Identity = 60/76 (78.95%), Postives = 69/76 (90.79%), Query Frame = 0

Query: 117 VMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWR 176
           VMLGIDLV+GDITVWDSLQ+ATPL+ LEKELKPMC ILP +LH+ GIF+VRP+LPV+PWR
Sbjct: 53  VMLGIDLVQGDITVWDSLQTATPLDELEKELKPMCTILPTLLHHGGIFSVRPDLPVVPWR 112

Query: 177 VRRVRTPQQSKATNCG 193
           VRRVR PQQS  T+CG
Sbjct: 113 VRRVRVPQQSSVTDCG 128

BLAST of Moc09g10030 vs. NCBI nr
Match: XP_022153247.1 (uncharacterized protein LOC111020782 [Momordica charantia])

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-21
Identity = 51/76 (67.11%), Postives = 63/76 (82.89%), Query Frame = 0

Query: 117 VMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWR 176
           VM+GIDLVEGD+TVWDSLQ+ TPLE LEK LKPMC I+P++LH+SG+ A+RP L  +PWR
Sbjct: 43  VMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPVILHWSGMLALRPILHTVPWR 102

Query: 177 VRRVRTPQQSKATNCG 193
           VRR   PQQ+  T+CG
Sbjct: 103 VRRCTVPQQAGFTDCG 118

BLAST of Moc09g10030 vs. NCBI nr
Match: XP_022155476.1 (uncharacterized protein LOC111022607 [Momordica charantia])

HSP 1 Score: 93.2 bits (230), Expect = 2.7e-15
Identity = 41/54 (75.93%), Postives = 50/54 (92.59%), Query Frame = 0

Query: 39  AGMQTKEWYHNLLDQTIELKDEVIDALVLFTTKKLEKCLHLCRKRFAIGDILLS 93
           AG+Q KEWY +LLD T++LKDEV+DALVLFT KKLEKC++LCRK+FAIGD+LLS
Sbjct: 181 AGLQGKEWYRDLLDPTVQLKDEVVDALVLFTAKKLEKCIYLCRKKFAIGDVLLS 234

BLAST of Moc09g10030 vs. NCBI nr
Match: XP_022156568.1 (uncharacterized protein LOC111023442 [Momordica charantia])

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-13
Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 0

Query: 117 VMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWR 176
           VM+ ID VEG+I VWDSL++ T   +LE++LK M  ++P +LH S + AVRPNLP+ PWR
Sbjct: 33  VMICIDFVEGEIVVWDSLRAITSYASLEEQLKVMHTVIPSLLHKSQVIAVRPNLPMTPWR 92

Query: 177 VRRV-RTPQQSKATNCG 193
           +RRV  TP+Q  + +CG
Sbjct: 93  IRRVTSTPEQFSSGDCG 109

BLAST of Moc09g10030 vs. ExPASy TrEMBL
Match: A0A6J1DY60 (uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025273 PE=3 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.8e-34
Identity = 85/192 (44.27%), Postives = 111/192 (57.81%), Query Frame = 0

Query: 40  GMQTKEWYHNLLDQTIELKDEVIDALVLFTTKKLEKCLHLCRKRFAIGDILLS------- 99
           G++ K W+  LLD   +L DE ID+L++ T +K+EKC HL R +FAIGD+LLS       
Sbjct: 16  GIKYKSWFGLLLDPQFQLDDEYIDSLMMLTARKVEKCKHLLRVQFAIGDVLLSNLLRRTD 75

Query: 100 --------------VNFAKPHKRSIRCHEAGSH-------------------GLEDQVML 159
                           +    +R+I  +  G                     G    VM+
Sbjct: 76  GPYAAMKPGVLPSKCTYDWRQERTIFRYVLGRQSDYDTLWSEADIVYTRMNIGGNHWVMI 135

Query: 160 GIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWRVRR 192
           GIDLVEGD+TVWDSLQ+ TPLE LEK LKPMC I+P +LH+SGI A+RPNLP++PWRVRR
Sbjct: 136 GIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGILALRPNLPMVPWRVRR 195

BLAST of Moc09g10030 vs. ExPASy TrEMBL
Match: A0A6J1D492 (uncharacterized protein LOC111016890 OS=Momordica charantia OX=3673 GN=LOC111016890 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.5e-27
Identity = 60/76 (78.95%), Postives = 69/76 (90.79%), Query Frame = 0

Query: 117 VMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWR 176
           VMLGIDLV+GDITVWDSLQ+ATPL+ LEKELKPMC ILP +LH+ GIF+VRP+LPV+PWR
Sbjct: 53  VMLGIDLVQGDITVWDSLQTATPLDELEKELKPMCTILPTLLHHGGIFSVRPDLPVVPWR 112

Query: 177 VRRVRTPQQSKATNCG 193
           VRRVR PQQS  T+CG
Sbjct: 113 VRRVRVPQQSSVTDCG 128

BLAST of Moc09g10030 vs. ExPASy TrEMBL
Match: A0A6J1DID7 (uncharacterized protein LOC111020782 OS=Momordica charantia OX=3673 GN=LOC111020782 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 9.2e-22
Identity = 51/76 (67.11%), Postives = 63/76 (82.89%), Query Frame = 0

Query: 117 VMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWR 176
           VM+GIDLVEGD+TVWDSLQ+ TPLE LEK LKPMC I+P++LH+SG+ A+RP L  +PWR
Sbjct: 43  VMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPVILHWSGMLALRPILHTVPWR 102

Query: 177 VRRVRTPQQSKATNCG 193
           VRR   PQQ+  T+CG
Sbjct: 103 VRRCTVPQQAGFTDCG 118

BLAST of Moc09g10030 vs. ExPASy TrEMBL
Match: A0A6J1DRS0 (uncharacterized protein LOC111022607 OS=Momordica charantia OX=3673 GN=LOC111022607 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.3e-15
Identity = 41/54 (75.93%), Postives = 50/54 (92.59%), Query Frame = 0

Query: 39  AGMQTKEWYHNLLDQTIELKDEVIDALVLFTTKKLEKCLHLCRKRFAIGDILLS 93
           AG+Q KEWY +LLD T++LKDEV+DALVLFT KKLEKC++LCRK+FAIGD+LLS
Sbjct: 181 AGLQGKEWYRDLLDPTVQLKDEVVDALVLFTAKKLEKCIYLCRKKFAIGDVLLS 234

BLAST of Moc09g10030 vs. ExPASy TrEMBL
Match: A0A6J1DQZ3 (uncharacterized protein LOC111023442 OS=Momordica charantia OX=3673 GN=LOC111023442 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 7.1e-14
Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 0

Query: 117 VMLGIDLVEGDITVWDSLQSATPLEALEKELKPMCMILPMVLHYSGIFAVRPNLPVMPWR 176
           VM+ ID VEG+I VWDSL++ T   +LE++LK M  ++P +LH S + AVRPNLP+ PWR
Sbjct: 33  VMICIDFVEGEIVVWDSLRAITSYASLEEQLKVMHTVIPSLLHKSQVIAVRPNLPMTPWR 92

Query: 177 VRRV-RTPQQSKATNCG 193
           +RRV  TP+Q  + +CG
Sbjct: 93  IRRVTSTPEQFSSGDCG 109

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158807.15.7e-3444.27uncharacterized protein LOC111025273 [Momordica charantia][more]
XP_022148137.15.2e-2778.95uncharacterized protein LOC111016890 [Momordica charantia][more]
XP_022153247.11.9e-2167.11uncharacterized protein LOC111020782 [Momordica charantia][more]
XP_022155476.12.7e-1575.93uncharacterized protein LOC111022607 [Momordica charantia][more]
XP_022156568.11.5e-1353.25uncharacterized protein LOC111023442 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DY602.8e-3444.27uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1D4922.5e-2778.95uncharacterized protein LOC111016890 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A6J1DID79.2e-2267.11uncharacterized protein LOC111020782 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DRS01.3e-1575.93uncharacterized protein LOC111022607 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DQZ37.1e-1453.25uncharacterized protein LOC111023442 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 117..192
e-value: 1.6E-4
score: 21.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g10030.1Moc09g10030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity