Moc09g09860 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g09860
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	CCHC-type domain-containing protein
Location	chr9: 8336994 .. 8337488 (+)
RNA-Seq Expression	Moc09g09860
Synteny	Moc09g09860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCATTATTTATGAGCCCAGTGAAGATTGATGTGAAGAAATTTGACGGAATGATCAACTTCGACTTTCGCAACTGCAAGTCAAAGATGTATCAGGAAAAATGCATCTCAAATCGGTTGAACTTGAAGGAGCAGTTTCACACACTGCGAATGGAGGAATGTATGAAAATTTTAGATCATCTAAGTAATCTCAATAGCATTATCTTTGAGCTGGAGGCAATCGAATTGAAGATAGATGACGAAGATAAGCCACTCAGACTCATCTTGTCACTTCCACCTTCTTATGAACACATGAAGCCGATTTTGATGTATGGAAAGGATAATTTGAATTTTGTCGAGGCTACTAGTAAACTATTGTCAGAAGAAAGAAGACTGAAGAGTGAAAAACGTACTTCACATGAAGATTCAACACTGATAGCTAGAAATTGGAAGAATAAAGACTCCGTGCAAAAGATCAAAGCTTGTTGTTTGGGATGCGACAATCTGGACAGATGA

mRNA sequence

ATGTCATTATTTATGAGCCCAGTGAAGATTGATGTGAAGAAATTTGACGGAATGATCAACTTCGACTTTCGCAACTGCAAGTCAAAGATGTATCAGGAAAAATGCATCTCAAATCGGTTGAACTTGAAGGAGCAGTTTCACACACTGCGAATGGAGGAATGTATGAAAATTTTAGATCATCTAAGTAATCTCAATAGCATTATCTTTGAGCTGGAGGCAATCGAATTGAAGATAGATGACGAAGATAAGCCACTCAGACTCATCTTGTCACTTCCACCTTCTTATGAACACATGAAGCCGATTTTGATGTATGGAAAGGATAATTTGAATTTTGTCGAGGCTACTAGTAAACTATTGTCAGAAGAAAGAAGACTGAAGAGTGAAAAACGTACTTCACATGAAGATTCAACACTGATAGCTAGAAATTGGAAGAATAAAGACTCCGTGCAAAAGATCAAAGCTTGTTGTTTGGGATGCGACAATCTGGACAGATGA

Coding sequence (CDS)

ATGTCATTATTTATGAGCCCAGTGAAGATTGATGTGAAGAAATTTGACGGAATGATCAACTTCGACTTTCGCAACTGCAAGTCAAAGATGTATCAGGAAAAATGCATCTCAAATCGGTTGAACTTGAAGGAGCAGTTTCACACACTGCGAATGGAGGAATGTATGAAAATTTTAGATCATCTAAGTAATCTCAATAGCATTATCTTTGAGCTGGAGGCAATCGAATTGAAGATAGATGACGAAGATAAGCCACTCAGACTCATCTTGTCACTTCCACCTTCTTATGAACACATGAAGCCGATTTTGATGTATGGAAAGGATAATTTGAATTTTGTCGAGGCTACTAGTAAACTATTGTCAGAAGAAAGAAGACTGAAGAGTGAAAAACGTACTTCACATGAAGATTCAACACTGATAGCTAGAAATTGGAAGAATAAAGACTCCGTGCAAAAGATCAAAGCTTGTTGTTTGGGATGCGACAATCTGGACAGATGA

Protein sequence

MSLFMSPVKIDVKKFDGMINFDFRNCKSKMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLILSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDSVQKIKACCLGCDNLDR

Homology

[Close]

BLAST of Moc09g09860 vs. NCBI nr
Match: XP_022139673.1 (uncharacterized protein LOC111010521 [Momordica charantia])

HSP 1 Score: 203.0 bits (515), Expect = 2.0e-48
Identity = 109/131 (83.21%), Postives = 113/131 (86.26%), Query Frame = 0


Query: 30  MYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLIL 89
           +YQ K ISNRL LKEQFHTL+MEE MKI DHLSNLNSIIFELEAIE+KIDDEDK LRLIL
Sbjct: 413 LYQAKGISNRLYLKEQFHTLQMEEGMKISDHLSNLNSIIFELEAIEVKIDDEDKALRLIL 472

Query: 90  SLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNW-KNKDS 149
           SLP SYEHMKPILMYGKD LNF E TSKLLSEERRLKSE RTSHEDS L+  NW K KDS
Sbjct: 473 SLPXSYEHMKPILMYGKDTLNFAEVTSKLLSEERRLKSEGRTSHEDSALVVSNWKKKKDS 532

Query: 150 VQKIKACCLGC 160
           VQK KACC GC
Sbjct: 533 VQK-KACCWGC 542

[Close]

BLAST of Moc09g09860 vs. NCBI nr
Match: KAF5758504.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 142.5 bits (358), Expect = 3.2e-30
Identity = 80/127 (62.99%), Postives = 93/127 (73.23%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           ++YQ K ISNRL LKEQFHTLRM+   KI DHLS LN+I+ ELEAI +K++DEDK LRLI
Sbjct: 101 QLYQGKGISNRLYLKEQFHTLRMDGDTKISDHLSVLNNIVSELEAIGVKVEDEDKALRLI 160

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
           LSL  SYEHMKPILMYGK+ L + + T KLLSEE+RL S   TS E + LI  N K K  
Sbjct: 161 LSLTSSYEHMKPILMYGKETLKYADVTEKLLSEEKRLGSSGHTSSEGTVLICGNGKKKHP 220

Query: 149 VQKIKAC 156
            QKI  C
Sbjct: 221 -QKIPVC 226

[Close]

BLAST of Moc09g09860 vs. NCBI nr
Match: KAE8736181.1 (hypothetical protein F3Y22_tig00000132pilonHSYRG00093 [Hibiscus syriacus])

HSP 1 Score: 142.1 bits (357), Expect = 4.2e-30
Identity = 77/131 (58.78%), Postives = 93/131 (70.99%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MYQ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ ELE+I ++IDDEDK LRLI
Sbjct: 107 EMYQAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSELESIGVEIDDEDKALRLI 166

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 167 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESGALSVFGNRKKYK 226

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 227 GSKRKIICWGC 237

[Close]

BLAST of Moc09g09860 vs. NCBI nr
Match: KAE8717380.1 (hypothetical protein F3Y22_tig00110050pilonHSYRG00143 [Hibiscus syriacus])

HSP 1 Score: 142.1 bits (357), Expect = 4.2e-30
Identity = 77/131 (58.78%), Postives = 93/131 (70.99%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MYQ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ ELE+I ++IDDEDK LRLI
Sbjct: 107 EMYQAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSELESIGVEIDDEDKALRLI 166

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 167 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESGALSVCGNRKKYK 226

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 227 GSKKKIICWGC 237

[Close]

BLAST of Moc09g09860 vs. NCBI nr
Match: KAE8732867.1 (hypothetical protein F3Y22_tig00001713pilonHSYRG00162 [Hibiscus syriacus])

HSP 1 Score: 141.4 bits (355), Expect = 7.2e-30
Identity = 77/131 (58.78%), Postives = 92/131 (70.23%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MYQ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ ELE+I + IDDEDK LRLI
Sbjct: 107 EMYQAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSELESIGVDIDDEDKALRLI 166

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 167 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESGALSVCGNRKKYK 226

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 227 GSKKKIICWGC 237

[Close]

BLAST of Moc09g09860 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.0e-11
Identity = 38/110 (34.55%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 30  MYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLIL 89
           +Y  K ++N+L LK+Q + L M E    L HL+  N +I +L  + +KI++EDK + L+ 
Sbjct: 91  LYMSKTLTNKLYLKKQLYALHMSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLN 150

Query: 90  SLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLI 140
           SLP SY+++   +++GK  +   + TS LL  E   K  K+  ++   LI
Sbjct: 151 SLPSSYDNLATTILHGKTTIELKDVTSALLLNE---KMRKKPENQGQALI 197

[Close]

BLAST of Moc09g09860 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-04
Identity = 32/118 (27.12%), Postives = 64/118 (54.24%), Query Frame = 0


Query: 30  MYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLIL 89
           +Y+ K ++++L L+++  +L++   M +L H    + +I EL A   KI++ DK   L++
Sbjct: 87  VYERKSLASQLALRKRLLSLKLSSEMSLLSHFHIFDELISELLAAGAKIEEMDKISHLLI 146

Query: 90  SLPPSYEH-MKPILMYGKDNLNFVEATSKLLSEERRLKSEKR-TSHEDSTLIARNWKN 146
           +LP  Y+  +  I    ++NL      ++LL +E ++K++   TS +    I  N  N
Sbjct: 147 TLPSCYDGIITAIETLSEENLTLAFVKNRLLDQEIKIKNDHNDTSKKVMNAIVHNNNN 204

[Close]

BLAST of Moc09g09860 vs. ExPASy TrEMBL
Match: A0A6J1CG82 (uncharacterized protein LOC111010521 OS=Momordica charantia OX=3673 GN=LOC111010521 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 9.7e-49
Identity = 109/131 (83.21%), Postives = 113/131 (86.26%), Query Frame = 0


Query: 30  MYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLIL 89
           +YQ K ISNRL LKEQFHTL+MEE MKI DHLSNLNSIIFELEAIE+KIDDEDK LRLIL
Sbjct: 413 LYQAKGISNRLYLKEQFHTLQMEEGMKISDHLSNLNSIIFELEAIEVKIDDEDKALRLIL 472

Query: 90  SLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNW-KNKDS 149
           SLP SYEHMKPILMYGKD LNF E TSKLLSEERRLKSE RTSHEDS L+  NW K KDS
Sbjct: 473 SLPXSYEHMKPILMYGKDTLNFAEVTSKLLSEERRLKSEGRTSHEDSALVVSNWKKKKDS 532

Query: 150 VQKIKACCLGC 160
           VQK KACC GC
Sbjct: 533 VQK-KACCWGC 542

[Close]

BLAST of Moc09g09860 vs. ExPASy TrEMBL
Match: A0A6A3BK59 (CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110050pilonHSYRG00143 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.0e-30
Identity = 77/131 (58.78%), Postives = 93/131 (70.99%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MYQ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ ELE+I ++IDDEDK LRLI
Sbjct: 107 EMYQAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSELESIGVEIDDEDKALRLI 166

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 167 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESGALSVCGNRKKYK 226

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 227 GSKKKIICWGC 237

[Close]

BLAST of Moc09g09860 vs. ExPASy TrEMBL
Match: A0A6A3DA47 (CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00000132pilonHSYRG00093 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.0e-30
Identity = 77/131 (58.78%), Postives = 93/131 (70.99%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MYQ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ ELE+I ++IDDEDK LRLI
Sbjct: 107 EMYQAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSELESIGVEIDDEDKALRLI 166

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 167 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESGALSVFGNRKKYK 226

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 227 GSKRKIICWGC 237

[Close]

BLAST of Moc09g09860 vs. ExPASy TrEMBL
Match: A0A6A3CWI3 (CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00001713pilonHSYRG00162 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 3.5e-30
Identity = 77/131 (58.78%), Postives = 92/131 (70.23%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MYQ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ ELE+I + IDDEDK LRLI
Sbjct: 107 EMYQAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSELESIGVDIDDEDKALRLI 166

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 167 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESGALSVCGNRKKYK 226

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 227 GSKKKIICWGC 237

[Close]

BLAST of Moc09g09860 vs. ExPASy TrEMBL
Match: A0A6A3C6U5 (G-type lectin S-receptor-like serine/threonine-protein kinase OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00010533pilonHSYRG00419 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.0e-29
Identity = 75/131 (57.25%), Postives = 93/131 (70.99%), Query Frame = 0


Query: 29  KMYQEKCISNRLNLKEQFHTLRMEECMKILDHLSNLNSIIFELEAIELKIDDEDKPLRLI 88
           +MY+ K +SNRL LKE+FH L+MEE  KI DHLS LN I+ E+E+I ++IDDEDK LRLI
Sbjct: 551 EMYKAKSLSNRLYLKEKFHKLQMEEGTKISDHLSALNGIVSEMESIGVEIDDEDKALRLI 610

Query: 89  LSLPPSYEHMKPILMYGKDNLNFVEATSKLLSEERRLKSEKRTSHEDSTLIARNWKNKDS 148
            SLP SYEHM+ +LMYGK+N+NF E TSKL+SEERRLK+ +  S E   L     + K  
Sbjct: 611 WSLPSSYEHMQTVLMYGKENVNFSEVTSKLISEERRLKNGENKSSESEALSVCGNRKKYK 670

Query: 149 VQKIKACCLGC 160
             K K  C GC
Sbjct: 671 GSKKKIICWGC 681

The following BLAST results are available for this feature:

BLAST of Moc09g09860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139673.1	2.0e-48	83.21	uncharacterized protein LOC111010521 [Momordica charantia]	[more]
KAF5758504.1	3.2e-30	62.99	putative RNA-directed DNA polymerase [Helianthus annuus]	[more]
KAE8736181.1	4.2e-30	58.78	hypothetical protein F3Y22_tig00000132pilonHSYRG00093 [Hibiscus syriacus]	[more]
KAE8717380.1	4.2e-30	58.78	hypothetical protein F3Y22_tig00110050pilonHSYRG00143 [Hibiscus syriacus]	[more]
KAE8732867.1	7.2e-30	58.78	hypothetical protein F3Y22_tig00001713pilonHSYRG00162 [Hibiscus syriacus]	[more]

BLAST of Moc09g09860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	2.0e-11	34.55	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	1.4e-04	27.12	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]

BLAST of Moc09g09860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CG82	9.7e-49	83.21	uncharacterized protein LOC111010521 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6A3BK59	2.0e-30	58.78	CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig0...	[more]
A0A6A3DA47	2.0e-30	58.78	CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig0...	[more]
A0A6A3CWI3	3.5e-30	58.78	CCHC-type domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig0...	[more]
A0A6A3C6U5	1.0e-29	57.25	G-type lectin S-receptor-like serine/threonine-protein kinase OS=Hibiscus syriac...	[more]

BLAST of Moc09g09860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 29..127 e-value: 3.5E-21 score: 75.4
None	No IPR available	PANTHER	PTHR34676	FAMILY NOT NAMED	coord: 29..158
None	No IPR available	PANTHER	PTHR34676:SF6	SUBFAMILY NOT NAMED	coord: 29..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g09860.1	Moc09g09860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0043170	macromolecule metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0044238	primary metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0008270	zinc ion binding