Moc09g09270 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g09270
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionprotein KINESIN LIGHT CHAIN-RELATED 1
Locationchr9: 7662628 .. 7665620 (-)
RNA-Seq ExpressionMoc09g09270
SyntenyMoc09g09270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAAGGCTTCAATTTCACTTCTCTCCCACTCCAATCGCCTGAATCTCCGCTCCATTTCATCCCTCATTTCCGATCCTCCCCCTTCTCCCCCTCCTTCTTCATCTCCTCTCTCCGCCGCCCATCTCAACCCCTGCTCCAAATCCCATGGCCTGTTCTTCAAACTCACCCGCTTCCAAGCAAACCCATGTCGGAATTTGGACACCCTTGTGGAGAAACGCCCTCAAATTTCATCCAGACAGAGGAAAATCAAAGAGAAGTCCGAGCTGGAGGAGGCTTTTGAGTCTGCAAACACTGTCGATGAAATGCTCAGCGCGTTCAAAGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGACCCTGAAAAGACTCTGTTGTATGCTTCTAGAGCTTTGAAAGCTTTGGACAATGATGATAATGGTGGTAAACCTTCTCTACTTGTTGCTATGACTTTGCAATTGATGGGTTCTGTTAATTATAGCTTGAAAAAGTTTAGTGATAGTTTAGGGTACCTGAATAGGGCTAATAGGGTACTGAGTCAGTTAGAGGAGAAGGGGTTTAGTGCTGCTGAGGATATTAGACCTGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGGAGAAGAGAAGAAGCTCTTGGTAATCTTAGGAAGTGTTTGGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTAGGGAAGGCGAATCGTGATTTGGCGGAAGCTTATGTTGCTATTTTGAACTTCAGGGATGCGCTTCCGTTTTGTATGAAGGCATTGGAGATACATAAGATGCAGTTGGGGCATAATTCAGTTGAAGTAGCCCATGATAGGAGGCTTCTAGGGGTGATTTATACTGGGTTGGACGAACATGAGAAGGCATTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAATTCCGATTTGCTTTGTGCGGAAATTGATGCTGCTAATATGCAAATTGCCCTGGGTCGGTACGATGAAGCCATTAGTACTCTCAAGGATGTTGCTCAACGTACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTTGACAAGAAAGAAGCAACTTCTCCAGTGGAGGCTGCTGAGGCGTATTCAGAAATATCGATGCAATATGAGACAATGAATGAGTTTGAAACTGCGATTTCTTTATTGAAAAGATCTCTAGCTGTGCTCGAGAAACTTCCACAAGAGCAACACTCAGAGGGTAGTGTTTCAGCTAGAATAGGGTGGTTACTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTGAAGGAGAGTTTTGGTTCCAAGCATTTTGGAGTTGGATATATATACAATAACCTCGGGGCAGCATATCTAGAGCTCGAGAGACCTCAATCGGCCGCGCAGATGTTTGCAATGGCAAAGGACATTATGGACGTATCACTTGGCCCTCATCATGCAGATTCAATTGAATCATGTCAGAATCTTTCAAAAGCATATGGTGCAATGGGAAGGTATGGTTACTTTCAAGTTTTTCTTTCGTCTCCATCACTAGCGACATTGTTACATTGTTTACAACGTATAATGTTTTACTACTAACTTGAACCTAGCTACCAAGTTGAACCTAGCTTGGTAGTCAAGGTATTTATACCTCTTCTTAAAGATCATACGTTCAAACCTCCAAAGGAAGTCATGATCAAACTTTAGCATTGTTTTTTTTTTTTGTATGAAACAATTTCACTGATGGTATGAAATTACTAACGGACAATACCTCCTTTTTAGTGTAGCTTCTACATAGCTATAATTGTATTTATAGGAAGAAATGGAGTTTCATTAGATTCTAAAAAAAAGTCTAACAGACTTCCTGAGGACTCTTACTGATAATTGAACTTTCAAACCTAATGGGTCGTGAATGCTGTAGAAGGCCTTTGAATTCAATCACGTGATCTTGAAGAAACTCGACTCCGACAGGAATGATGACTATCTCATGATTATTTTGAATAGGAAATGTTACAGGGAAGAGGACTCTTGGTGGTCTAGTTTTGGAAAAGAAATGAATATTAACCACTGTGCGTTGGTCAAGTGGTCAATATGACCAATGAGAAAAGTAAAAGATTTAGAGGGAATGCGTTTAAACCATGGTGACCACCTACTTGAGATTTAACACTATACGAATTCTTCTGGTAACCAAATGTAACAGGGTCAAAAAATTGTCCCACAAGATTAATTGAGTGCACGGGAATTGACTTGGACAGTCATAAATATTAAAAGGACTATAGATATCCTGATATGAAATAACTAAGTCATCTTTGGAGATGATTGCTTGTGGAACTAATTTTATATCGTGATTGGGACTAACGGACTTGATAAATTTTAGATGATTTTTACCATGAAGGTATTAAAACACCATTCCCAGTCCTTCCTCTTTCTTGTTGCCGTTGGCATGTTGTCATTTGTTATTGAATGATGCAAAAATGTTGCAGAAAGGTTTACTTTTCTAACAGATATTCAATGATCACACGAGTAGCTCAACAGCATATTGGCTATTGAGGGACATTCTTGAACTTCATTAATTCAATCCTTTTTCTGTGTGTTGGTGCAGTTATGCCCTTGCAATTGATTTCCAAGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCAAGTGCAGAAGATGAACTTAAAGAAGCCCAACGGATGCTTGAACAATTAAAGAGGAAAGCACACGGTACGTCAAAACCCGAGCTTTCGACGAAGGCCTTGCCACTACCTCATTCCCCAACATCTTCCAAAAGTTTGCAGCCCAATATTCCCGGCAGTCAGAAGCAACTATAG

mRNA sequence

ATGAGAAAGGCTTCAATTTCACTTCTCTCCCACTCCAATCGCCTGAATCTCCGCTCCATTTCATCCCTCATTTCCGATCCTCCCCCTTCTCCCCCTCCTTCTTCATCTCCTCTCTCCGCCGCCCATCTCAACCCCTGCTCCAAATCCCATGGCCTGTTCTTCAAACTCACCCGCTTCCAAGCAAACCCATGTCGGAATTTGGACACCCTTGTGGAGAAACGCCCTCAAATTTCATCCAGACAGAGGAAAATCAAAGAGAAGTCCGAGCTGGAGGAGGCTTTTGAGTCTGCAAACACTGTCGATGAAATGCTCAGCGCGTTCAAAGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGACCCTGAAAAGACTCTGTTGTATGCTTCTAGAGCTTTGAAAGCTTTGGACAATGATGATAATGGTGGTAAACCTTCTCTACTTGTTGCTATGACTTTGCAATTGATGGGTTCTGTTAATTATAGCTTGAAAAAGTTTAGTGATAGTTTAGGGTACCTGAATAGGGCTAATAGGGTACTGAGTCAGTTAGAGGAGAAGGGGTTTAGTGCTGCTGAGGATATTAGACCTGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGGAGAAGAGAAGAAGCTCTTGGTAATCTTAGGAAGTGTTTGGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTAGGGAAGGCGAATCGTGATTTGGCGGAAGCTTATGTTGCTATTTTGAACTTCAGGGATGCGCTTCCGTTTTGTATGAAGGCATTGGAGATACATAAGATGCAGTTGGGGCATAATTCAGTTGAAGTAGCCCATGATAGGAGGCTTCTAGGGGTGATTTATACTGGGTTGGACGAACATGAGAAGGCATTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAATTCCGATTTGCTTTGTGCGGAAATTGATGCTGCTAATATGCAAATTGCCCTGGGTCGGTACGATGAAGCCATTAGTACTCTCAAGGATGTTGCTCAACGTACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTTGACAAGAAAGAAGCAACTTCTCCAGTGGAGGCTGCTGAGGCGTATTCAGAAATATCGATGCAATATGAGACAATGAATGAGTTTGAAACTGCGATTTCTTTATTGAAAAGATCTCTAGCTGTGCTCGAGAAACTTCCACAAGAGCAACACTCAGAGGGTAGTGTTTCAGCTAGAATAGGGTGGTTACTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTGAAGGAGAGTTTTGGTTCCAAGCATTTTGGAGTTGGATATATATACAATAACCTCGGGGCAGCATATCTAGAGCTCGAGAGACCTCAATCGGCCGCGCAGATGTTTGCAATGGCAAAGGACATTATGGACGTATCACTTGGCCCTCATCATGCAGATTCAATTGAATCATGTCAGAATCTTTCAAAAGCATATGGTGCAATGGGAAGTTATGCCCTTGCAATTGATTTCCAAGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCAAGTGCAGAAGATGAACTTAAAGAAGCCCAACGGATGCTTGAACAATTAAAGAGGAAAGCACACGGTACGTCAAAACCCGAGCTTTCGACGAAGGCCTTGCCACTACCTCATTCCCCAACATCTTCCAAAAGTTTGCAGCCCAATATTCCCGGCAGTCAGAAGCAACTATAG

Coding sequence (CDS)

ATGAGAAAGGCTTCAATTTCACTTCTCTCCCACTCCAATCGCCTGAATCTCCGCTCCATTTCATCCCTCATTTCCGATCCTCCCCCTTCTCCCCCTCCTTCTTCATCTCCTCTCTCCGCCGCCCATCTCAACCCCTGCTCCAAATCCCATGGCCTGTTCTTCAAACTCACCCGCTTCCAAGCAAACCCATGTCGGAATTTGGACACCCTTGTGGAGAAACGCCCTCAAATTTCATCCAGACAGAGGAAAATCAAAGAGAAGTCCGAGCTGGAGGAGGCTTTTGAGTCTGCAAACACTGTCGATGAAATGCTCAGCGCGTTCAAAGACATGGAATCCGCTTTTGAAGAGAGGGATCTTGGATTGGCTTCCTTGAAAATTGGTCTGAAACTTGACCAAGAAGGTGAGGACCCTGAAAAGACTCTGTTGTATGCTTCTAGAGCTTTGAAAGCTTTGGACAATGATGATAATGGTGGTAAACCTTCTCTACTTGTTGCTATGACTTTGCAATTGATGGGTTCTGTTAATTATAGCTTGAAAAAGTTTAGTGATAGTTTAGGGTACCTGAATAGGGCTAATAGGGTACTGAGTCAGTTAGAGGAGAAGGGGTTTAGTGCTGCTGAGGATATTAGACCTGTGCTTCATGCTGTGCAACTTGAGTTGGCAAATGTTAAGACTGCAATGGGGAGAAGAGAAGAAGCTCTTGGTAATCTTAGGAAGTGTTTGGAGATCAAGGAGTTGACCTTGGAGAAGGATAGTAGAGAATTAGGGAAGGCGAATCGTGATTTGGCGGAAGCTTATGTTGCTATTTTGAACTTCAGGGATGCGCTTCCGTTTTGTATGAAGGCATTGGAGATACATAAGATGCAGTTGGGGCATAATTCAGTTGAAGTAGCCCATGATAGGAGGCTTCTAGGGGTGATTTATACTGGGTTGGACGAACATGAGAAGGCATTGGAGCAGAATGAATTGTCTCAGAAGGTTTTGAAGAACTGGGGCCTTAATTCCGATTTGCTTTGTGCGGAAATTGATGCTGCTAATATGCAAATTGCCCTGGGTCGGTACGATGAAGCCATTAGTACTCTCAAGGATGTTGCTCAACGTACAGATAAAGACAGTGAAACTCGGGCAGTGGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTTGACAAGAAAGAAGCAACTTCTCCAGTGGAGGCTGCTGAGGCGTATTCAGAAATATCGATGCAATATGAGACAATGAATGAGTTTGAAACTGCGATTTCTTTATTGAAAAGATCTCTAGCTGTGCTCGAGAAACTTCCACAAGAGCAACACTCAGAGGGTAGTGTTTCAGCTAGAATAGGGTGGTTACTACTGTTGACCGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTGAAGGAGAGTTTTGGTTCCAAGCATTTTGGAGTTGGATATATATACAATAACCTCGGGGCAGCATATCTAGAGCTCGAGAGACCTCAATCGGCCGCGCAGATGTTTGCAATGGCAAAGGACATTATGGACGTATCACTTGGCCCTCATCATGCAGATTCAATTGAATCATGTCAGAATCTTTCAAAAGCATATGGTGCAATGGGAAGTTATGCCCTTGCAATTGATTTCCAAGAGCAAGTGGTTGATGCTTGGGAAAGCCATGGCTCAAGTGCAGAAGATGAACTTAAAGAAGCCCAACGGATGCTTGAACAATTAAAGAGGAAAGCACACGGTACGTCAAAACCCGAGCTTTCGACGAAGGCCTTGCCACTACCTCATTCCCCAACATCTTCCAAAAGTTTGCAGCCCAATATTCCCGGCAGTCAGAAGCAACTATAG

Protein sequence

MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHGTSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL
Homology
BLAST of Moc09g09270 vs. NCBI nr
Match: XP_022143612.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Momordica charantia])

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 634/634 (100.00%), Postives = 634/634 (100.00%), Query Frame = 0

Query: 1   MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQ 60
           MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQ
Sbjct: 1   MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQ 60

Query: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG 120
           ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG
Sbjct: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG 120

Query: 121 LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK 180
           LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK
Sbjct: 121 LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK 180

Query: 181 FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC 240
           FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC
Sbjct: 181 FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC 240

Query: 241 LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD 300
           LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD
Sbjct: 241 LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD 300

Query: 301 RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST 360
           RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST
Sbjct: 301 RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST 360

Query: 361 LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA 420
           LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA
Sbjct: 361 LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA 420

Query: 421 YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP 480
           YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP
Sbjct: 421 YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP 480

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA 540
           YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA 540

Query: 541 DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG 600
           DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG
Sbjct: 541 DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG 600

Query: 601 TSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL 635
           TSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL
Sbjct: 601 TSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL 634

BLAST of Moc09g09270 vs. NCBI nr
Match: XP_038899312.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.4e-307
Identity = 561/634 (88.49%), Postives = 595/634 (93.85%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSLISDPPPS--PPPSSSPLSAAHLNPCSKSHGLFFKLTRF 61
           R ASISLLSHSNRLNLRS+SS IS    S   P +SSP+S  +L PC+K+HGLFFK TRF
Sbjct: 3   RNASISLLSHSNRLNLRSLSSFISSNSISNPSPSNSSPVSTPNLKPCTKNHGLFFKFTRF 62

Query: 62  QANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDL 121
           QANP RNLDTLVEKRPQISSRQRK+KEKSELEEAFESA+TVDEM+ AFKDMESAFEERDL
Sbjct: 63  QANPSRNLDTLVEKRPQISSRQRKVKEKSELEEAFESADTVDEMVKAFKDMESAFEERDL 122

Query: 122 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLK 181
           GLASLKIGLKLDQEGEDPE  LLYA+RALKALD D N G PSL VAMTLQLMGSVNYSLK
Sbjct: 123 GLASLKIGLKLDQEGEDPENILLYANRALKALDKDVN-GNPSLPVAMTLQLMGSVNYSLK 182

Query: 182 KFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRK 241
           +FSDSLGYLNRANR+L QL EKGFS AED+RPVLHAVQLELANVKTAMGRREEAL NLRK
Sbjct: 183 RFSDSLGYLNRANRILGQLVEKGFS-AEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 242

Query: 242 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAH 301
           CLEIKELTLEKDSRELG ANRDLAEAYVA+LNF+DALPFCMKALEIHKMQLGHNSVEVAH
Sbjct: 243 CLEIKELTLEKDSRELGNANRDLAEAYVAVLNFKDALPFCMKALEIHKMQLGHNSVEVAH 302

Query: 302 DRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIS 361
           DRRLL VIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGRY+EAIS
Sbjct: 303 DRRLLAVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYNEAIS 362

Query: 362 TLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 421
           TLKDV Q TDKDSETRAVVFISMGKVLCNQEKFTD KRCLEIASGIFDK+EATSPVEAAE
Sbjct: 363 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDPKRCLEIASGIFDKREATSPVEAAE 422

Query: 422 AYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAI 481
           AYSEISMQYETMNEFETAISLLK+SLA+LEKLP EQHSEGSVSAR+GWLLLLTGKVQEAI
Sbjct: 423 AYSEISMQYETMNEFETAISLLKKSLALLEKLPHEQHSEGSVSARLGWLLLLTGKVQEAI 482

Query: 482 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 541
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 483 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 542

Query: 542 ADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAH 601
           ADSIE+C+NLSKAYGAMGSY LAI+FQEQVVDAWESHGS+AEDEL+EAQ+ML+QLKRKAH
Sbjct: 543 ADSIEACRNLSKAYGAMGSYPLAIEFQEQVVDAWESHGSTAEDELREAQQMLKQLKRKAH 602

Query: 602 GTSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQ 634
           GTSK ELSTKALPLPHS TSSK+LQP++P  QK+
Sbjct: 603 GTSKIELSTKALPLPHSSTSSKTLQPDLPARQKR 634

BLAST of Moc09g09270 vs. NCBI nr
Match: XP_022954096.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata])

HSP 1 Score: 1054.3 bits (2725), Expect = 4.2e-304
Identity = 559/636 (87.89%), Postives = 593/636 (93.24%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSL-----ISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKL 61
           R ASISLL HSNR+NLRS+SSL     ISDP PS    SSP+SA HLNPC+K+HGLFFKL
Sbjct: 3   RNASISLLFHSNRINLRSLSSLISPKFISDPSPS---YSSPVSAPHLNPCTKNHGLFFKL 62

Query: 62  TRFQANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEE 121
           +RFQANP RNL+TLVEKRPQISSRQRK KEKSELEEAFESANTVDEM+ AFKDMES FEE
Sbjct: 63  SRFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEE 122

Query: 122 RDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNY 181
           RDLGLASLKIGLKL+QE EDPEK L+YA+RALKALD DDN GKPSL VAMTLQL+GSVNY
Sbjct: 123 RDLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDN-GKPSLPVAMTLQLLGSVNY 182

Query: 182 SLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGN 241
           SLK+FSDSLGYLNRANR+L QLEE GFS AE IRPVLHAVQLELANVKTAMGRREEAL N
Sbjct: 183 SLKRFSDSLGYLNRANRILGQLEESGFS-AEGIRPVLHAVQLELANVKTAMGRREEALAN 242

Query: 242 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVE 301
           LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNF+DALPFCM+ALEIHK QLGHNSVE
Sbjct: 243 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVE 302

Query: 302 VAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDE 361
           VAHDRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGRYDE
Sbjct: 303 VAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDE 362

Query: 362 AISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVE 421
           AISTLKDV Q T+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVE
Sbjct: 363 AISTLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVE 422

Query: 422 AAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQ 481
           AAEAYSEISMQYE+MNEFETAISLLKRSLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKV 
Sbjct: 423 AAEAYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVH 482

Query: 482 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG 541
           EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLG
Sbjct: 483 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLG 542

Query: 542 PHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKR 601
           PHHADSIE+CQNL+KAYG++GSYALAI+FQEQVVDAWE HGS+AEDEL+EAQRMLEQLKR
Sbjct: 543 PHHADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKR 602

Query: 602 KAHGTSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           KAHG SK ELS KALPLPHS TSSK+LQP+IP SQK
Sbjct: 603 KAHGKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Moc09g09270 vs. NCBI nr
Match: XP_023549125.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.6e-303
Identity = 557/636 (87.58%), Postives = 591/636 (92.92%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSL-----ISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKL 61
           R ASISLL HSNR+NLRS+SSL     ISDP PS    SSP+SA HLNPC+K+HGLFFK 
Sbjct: 3   RNASISLLFHSNRINLRSLSSLISPKFISDPSPS---YSSPVSAPHLNPCTKNHGLFFKF 62

Query: 62  TRFQANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEE 121
            RFQA+P RNL+TLVEKRPQISSRQRK KEKSELEEAFESANTVDEM+ AFKDMES FEE
Sbjct: 63  NRFQASPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEE 122

Query: 122 RDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNY 181
           RDLGLASLKIGLKL+QE EDPEK L+YA+RALKALD DDN GKPSL VAMTLQL+GSVNY
Sbjct: 123 RDLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDN-GKPSLPVAMTLQLLGSVNY 182

Query: 182 SLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGN 241
           SLK+FSDSLGYLNRANR+L QLEE GFS AE IRPVLHAVQLELANVKTAMGRREEAL N
Sbjct: 183 SLKRFSDSLGYLNRANRILGQLEESGFS-AEGIRPVLHAVQLELANVKTAMGRREEALAN 242

Query: 242 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVE 301
           LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNF+DALPFCM+ALEIHK QLGHNSVE
Sbjct: 243 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVE 302

Query: 302 VAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDE 361
           VAHDRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGRYDE
Sbjct: 303 VAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDE 362

Query: 362 AISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVE 421
           AISTLKDV Q T+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPV 
Sbjct: 363 AISTLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVA 422

Query: 422 AAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQ 481
           AAEAYSEISMQYE+MNEFETAISLLKRSLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKV 
Sbjct: 423 AAEAYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVH 482

Query: 482 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG 541
           EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG
Sbjct: 483 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG 542

Query: 542 PHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKR 601
           PHHADSIE+CQNL+KAYG++GSYALAI+FQEQVVDAWE HGS+AEDEL+EAQRMLEQLKR
Sbjct: 543 PHHADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSTAEDELREAQRMLEQLKR 602

Query: 602 KAHGTSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           KAHG SK ELS KALPLPHS TSSK+LQP+IP SQK
Sbjct: 603 KAHGKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Moc09g09270 vs. NCBI nr
Match: KAG7014584.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.8e-302
Identity = 555/636 (87.26%), Postives = 593/636 (93.24%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSL-----ISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKL 61
           R ASISLL HSNR+NLRS+SSL     ISDP PS    SSP+SA+HLNPC+K+HGLFFK 
Sbjct: 13  RNASISLLFHSNRINLRSLSSLISPKFISDPSPS---YSSPVSASHLNPCTKNHGLFFKF 72

Query: 62  TRFQANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEE 121
           +RFQANP RNL+TLVEKRPQISSRQRK KEKSELEEAFESANTVDEM+ AFKDMES FEE
Sbjct: 73  SRFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEE 132

Query: 122 RDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNY 181
           RDLGLASLKIGLKL+QE EDPEK L+YA+RALKALD DDN GKPSL VAMTLQL+GSVNY
Sbjct: 133 RDLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDN-GKPSLPVAMTLQLLGSVNY 192

Query: 182 SLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGN 241
           SLK+FSDSLGYLNRANR+L QLEE GFS AE IRPVLHAVQLELANVKTAMGRREEAL +
Sbjct: 193 SLKRFSDSLGYLNRANRILGQLEESGFS-AEGIRPVLHAVQLELANVKTAMGRREEALAS 252

Query: 242 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVE 301
           LRKCLEIKELTLEKDSREL KANRDLAEAYVAILNF+DALPFCM+ALEIHK QLGHNSVE
Sbjct: 253 LRKCLEIKELTLEKDSRELAKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVE 312

Query: 302 VAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDE 361
           VAHDRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGR+DE
Sbjct: 313 VAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRFDE 372

Query: 362 AISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVE 421
           AISTLKDV Q T+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA+SPVE
Sbjct: 373 AISTLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEASSPVE 432

Query: 422 AAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQ 481
           AAEAYSEISMQYE+MNEFETAISLLKRSLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKV 
Sbjct: 433 AAEAYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVH 492

Query: 482 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG 541
           EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG
Sbjct: 493 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG 552

Query: 542 PHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKR 601
           PHHADSIE+CQNL+KAYG++ SYALAI+FQEQVVDAWE HGS+AEDEL+EAQRMLEQLKR
Sbjct: 553 PHHADSIEACQNLAKAYGSLESYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKR 612

Query: 602 KAHGTSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           KAHG SK ELS KALPLPHS TSSK+LQP+IP SQK
Sbjct: 613 KAHGKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 643

BLAST of Moc09g09270 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.9e-44
Identity = 153/554 (27.62%), Postives = 265/554 (47.83%), Query Frame = 0

Query: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMES-AFEERDL 120
           +NP R   T ++K P  SS   + K         + + TV    +A  D++  + +  DL
Sbjct: 30  SNPPR---TPMKKTP--SSTPSRSKPSPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDL 89

Query: 121 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDN----------DDNGGKPSLLVAMTLQ 180
           G   LK+       GE P K L YA RA K+ +             + G P L +AM+L 
Sbjct: 90  GPFLLKLARDAIASGEGPNKALDYAIRATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLH 149

Query: 181 LMGSVNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGR 240
           ++ ++  SL +F +++  L RA +V         S A        +  ++L +  + +G+
Sbjct: 150 VLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHSLA------AFSGHMQLGDTLSMLGQ 209

Query: 241 REEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQ 300
            + ++    + L+I+  TL      +G+  R LAEAYV  + F  A   C K LEIH+  
Sbjct: 210 IDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAH 269

Query: 301 LGHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQI 360
               S+E A DRRL+ +I     ++E ALE   L+   +   G  S++   ++   N+ +
Sbjct: 270 SEPASLEEAADRRLMAIICEAKGDYENALEHLVLASMAMIASGQESEVASIDVSIGNIYM 329

Query: 361 ALGRYDEAI----STLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGI 420
           +L R+DEA+      L        +   T A VF+ + ++     K  ++K   E A  I
Sbjct: 330 SLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGKLRESKSYCENALRI 389

Query: 421 FDKK-EATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSAR 480
           ++K    T+  E A   +EIS  YE+++E E A+ LL++S+ +LE  P +Q +   + AR
Sbjct: 390 YNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKSMKLLEDKPGQQSAIAGLEAR 449

Query: 481 IGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMF 540
           +G +    G+ ++A    E A   L+ + G K    G + N +G A ++L +   A ++F
Sbjct: 450 MGVMYYTVGRYEDARNAFESAVTKLRAA-GEKSAFFGVVLNQMGLACVQLFKIDEAGELF 509

Query: 541 AMAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDEL 599
             A+ I++   GP   D++    NL+  Y AMG    AI+  EQV+   E    +A  + 
Sbjct: 510 EEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIEDAIEILEQVLKLREEKLGTANPDF 569

BLAST of Moc09g09270 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 1.2e-43
Identity = 145/520 (27.88%), Postives = 244/520 (46.92%), Query Frame = 0

Query: 115 EERDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKP-SLLVAMTLQLMGS 174
           E  +LG+  LK   +L   GE+  K L  A RA+K  +    G K   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 175 VNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEA 234
           +   L +++D++  L R+      +E       ED      A  ++L ++   MG+ E +
Sbjct: 209 IYAGLGRYNDAVPVLERS------IEIPMIEDGEDHALAKFAGCMQLGDMYGLMGQVENS 268

Query: 235 LGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQ--LG 294
           +      LEI+   L +    +G+  R LAEA+V  + F +A   C  AL+IHK      
Sbjct: 269 IMLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAA 328

Query: 295 HNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIAL 354
             S+E A DR+L+G+I     ++E ALE   L+   + +     D+   +    +  ++L
Sbjct: 329 TASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSL 388

Query: 355 GRYDEAI----STLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFD 414
            R+DEAI      L    Q   +   + A+V++ +  +     K  D+K   E A  I+ 
Sbjct: 389 ARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYL 448

Query: 415 KKEATSPV-EAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIG 474
           K    +P+ E A  + EIS  Y++MNE + A+ LL+R+L +    P +Q++   + A++G
Sbjct: 449 KPTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMG 508

Query: 475 WLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAM 534
            +  + G   E+    + A    + S   K    G   N +G A ++      AA +F  
Sbjct: 509 VVTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEE 568

Query: 535 AKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELK- 594
           AK I++   GP+H D++    NL+  Y AMG    AI+  E VV   E    +A  E++ 
Sbjct: 569 AKTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVED 628

Query: 595 EAQRMLEQLKRKAHGTSKPELSTKALPLPHSPTSSKSLQP 626
           E QR+   LK    G SK   +   L L ++P  +   +P
Sbjct: 629 EKQRLAALLKEAGRGRSKRNRALLTL-LDNNPEIANGQRP 661

BLAST of Moc09g09270 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.6e-40
Identity = 141/519 (27.17%), Postives = 243/519 (46.82%), Query Frame = 0

Query: 111 ESAFEERDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQL 170
           E + E  +L    L     L   G+   K L    RA K  +     GKP L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 171 MGSVNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRR 230
             +V+  LK++++++  L R+  +   + E+G    E+      A  ++L +    +G+ 
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEI--PVVEEG----EEHALAKFAGLMQLGDTYAMVGQL 257

Query: 231 EEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQL 290
           E ++    + L I++  L ++   +G+  R LAEA V  L F +A   C  AL IH+   
Sbjct: 258 ESSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESG 317

Query: 291 GHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIA 350
              S+  A DRRL+G+I     +HE ALE   L+   +   G  S++   +    +  ++
Sbjct: 318 LPGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLS 377

Query: 351 LGRYDEAISTLKD--VAQRTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIF 410
           L R+DEAI   +    A +T K     AV  V+I +  +     K  +AK   E A  I+
Sbjct: 378 LSRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIY 437

Query: 411 DKKE-ATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARI 470
           +      SP E A   ++IS+  E+MNE E AI+LL+++L +    P ++     + A++
Sbjct: 438 ESHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQM 497

Query: 471 GWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 530
           G L  + GK  E+    + A   L+ +   +    G   N +G A ++L+  + A ++F 
Sbjct: 498 GVLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFE 557

Query: 531 MAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELK 590
            AK I++   GP+H +++    NL+ AY A+G    AI     VV   E    +A    +
Sbjct: 558 EAKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTE 617

Query: 591 EAQRMLEQLKRKAHGTSKPELSTKALPLPHSPTSSKSLQ 625
           + +R L QL ++A   +  +  +    +    TSS +L+
Sbjct: 618 DEKRRLAQLLKEAGNVTGRKAKSLKTLIDSDLTSSSALR 650

BLAST of Moc09g09270 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 6.2e-16
Identity = 79/353 (22.38%), Postives = 153/353 (43.34%), Query Frame = 0

Query: 227  MGRREEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIH 286
            +G   +A+  L++ LEI+E  L+ D   + ++   LA  YV    F +A     +ALEI 
Sbjct: 940  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYVQSKKFGNAEQLYKQALEIS 999

Query: 287  KMQLGHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAAN 346
            +   G   + VA +   L V+Y   ++ E+A +  + S K+ +          A    + 
Sbjct: 1000 ENAYGSEHMRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1059

Query: 347  MQIALGRYDEAISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIF 406
               AL R       L+       KD+   A     +G +   Q     A+  L+ +  + 
Sbjct: 1060 YGFALLRR----RALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1119

Query: 407  DKKEATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSAR-I 466
            ++       + A++ + ++  Y    +++ A  L +R+L +  +     H   + + + +
Sbjct: 1120 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1179

Query: 467  GWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 526
              L    GK+ +A+P  E A E  ++SFG KH  V     NL   Y ++++   A+ ++ 
Sbjct: 1180 AVLYKRKGKLDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPLYE 1239

Query: 527  MAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGS 579
             A  I + SLG  H    E+ +NL+      G +  A +  ++ ++  E+  S
Sbjct: 1240 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDFEKAAELYKRAMEIKETETS 1280

BLAST of Moc09g09270 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.4e-15
Identity = 78/353 (22.10%), Postives = 152/353 (43.06%), Query Frame = 0

Query: 227  MGRREEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIH 286
            +G   +A+  L++ LEI+E  L+ D   + ++   LA  Y+    F +A     +ALEI 
Sbjct: 929  LGLLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYMQSKKFGNAEQLYKQALEIS 988

Query: 287  KMQLGHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAAN 346
            +   G   + VA +   L V+Y   ++ E+A +  + S K+ +          A    + 
Sbjct: 989  ENAYGSEHLRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQK--------SARRKGSM 1048

Query: 347  MQIALGRYDEAISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIF 406
               AL R       L+       KD+   A     +G +   Q     A+  L+ +  + 
Sbjct: 1049 YGFALLRR----RALQLEELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMR 1108

Query: 407  DKKEATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSAR-I 466
            ++       + A++ + ++  Y    +++ A  L +R+L +  +     H   + + + +
Sbjct: 1109 ERVLGADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHL 1168

Query: 467  GWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 526
              L    GK+ +A+P  E A +  ++SFG KH  V     NL   Y ++++   A  ++ 
Sbjct: 1169 AVLYKRKGKLDKAVPLYELAVDIRQKSFGPKHPSVATALVNLAVLYCQMKKQDDALPLYE 1228

Query: 527  MAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGS 579
             A  I + SLG  H    E+ +NL+      G Y  A +  ++ ++  E+  S
Sbjct: 1229 RAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGDYEKAAELYKRAMEIKETETS 1269

BLAST of Moc09g09270 vs. ExPASy TrEMBL
Match: A0A6J1CRC1 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Momordica charantia OX=3673 GN=LOC111013468 PE=4 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 634/634 (100.00%), Postives = 634/634 (100.00%), Query Frame = 0

Query: 1   MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQ 60
           MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQ
Sbjct: 1   MRKASISLLSHSNRLNLRSISSLISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKLTRFQ 60

Query: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG 120
           ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG
Sbjct: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG 120

Query: 121 LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK 180
           LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK
Sbjct: 121 LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK 180

Query: 181 FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC 240
           FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC
Sbjct: 181 FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC 240

Query: 241 LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD 300
           LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD
Sbjct: 241 LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD 300

Query: 301 RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST 360
           RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST
Sbjct: 301 RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST 360

Query: 361 LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA 420
           LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA
Sbjct: 361 LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA 420

Query: 421 YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP 480
           YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP
Sbjct: 421 YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP 480

Query: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA 540
           YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA 540

Query: 541 DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG 600
           DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG
Sbjct: 541 DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG 600

Query: 601 TSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL 635
           TSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL
Sbjct: 601 TSKPELSTKALPLPHSPTSSKSLQPNIPGSQKQL 634

BLAST of Moc09g09270 vs. ExPASy TrEMBL
Match: A0A6J1GPX7 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456463 PE=4 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 2.0e-304
Identity = 559/636 (87.89%), Postives = 593/636 (93.24%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSL-----ISDPPPSPPPSSSPLSAAHLNPCSKSHGLFFKL 61
           R ASISLL HSNR+NLRS+SSL     ISDP PS    SSP+SA HLNPC+K+HGLFFKL
Sbjct: 3   RNASISLLFHSNRINLRSLSSLISPKFISDPSPS---YSSPVSAPHLNPCTKNHGLFFKL 62

Query: 62  TRFQANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEE 121
           +RFQANP RNL+TLVEKRPQISSRQRK KEKSELEEAFESANTVDEM+ AFKDMES FEE
Sbjct: 63  SRFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEE 122

Query: 122 RDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNY 181
           RDLGLASLKIGLKL+QE EDPEK L+YA+RALKALD DDN GKPSL VAMTLQL+GSVNY
Sbjct: 123 RDLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDN-GKPSLPVAMTLQLLGSVNY 182

Query: 182 SLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGN 241
           SLK+FSDSLGYLNRANR+L QLEE GFS AE IRPVLHAVQLELANVKTAMGRREEAL N
Sbjct: 183 SLKRFSDSLGYLNRANRILGQLEESGFS-AEGIRPVLHAVQLELANVKTAMGRREEALAN 242

Query: 242 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVE 301
           LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNF+DALPFCM+ALEIHK QLGHNSVE
Sbjct: 243 LRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVE 302

Query: 302 VAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDE 361
           VAHDRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGRYDE
Sbjct: 303 VAHDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDE 362

Query: 362 AISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVE 421
           AISTLKDV Q T+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVE
Sbjct: 363 AISTLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVE 422

Query: 422 AAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQ 481
           AAEAYSEISMQYE+MNEFETAISLLKRSLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKV 
Sbjct: 423 AAEAYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVH 482

Query: 482 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLG 541
           EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELER QSAAQMFAMAKDIMDVSLG
Sbjct: 483 EAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLG 542

Query: 542 PHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKR 601
           PHHADSIE+CQNL+KAYG++GSYALAI+FQEQVVDAWE HGS+AEDEL+EAQRMLEQLKR
Sbjct: 543 PHHADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKR 602

Query: 602 KAHGTSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           KAHG SK ELS KALPLPHS TSSK+LQP+IP SQK
Sbjct: 603 KAHGKSKIELSMKALPLPHSSTSSKTLQPDIPSSQK 633

BLAST of Moc09g09270 vs. ExPASy TrEMBL
Match: A0A6J1JWL0 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC111488134 PE=4 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.7e-299
Identity = 551/633 (87.05%), Postives = 584/633 (92.26%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSLISDPPPSPP--PSSSPLSAAHLNPCSKSHGLFFKLTRF 61
           R ASISLL HSNR NLRS+SSLIS    S P    SSP+S  HLNPC+K+HGLFFK  RF
Sbjct: 3   RNASISLLFHSNRFNLRSLSSLISPKFISDPSFSYSSPVSTTHLNPCTKNHGLFFKFKRF 62

Query: 62  QANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDL 121
           QANP RNL+TLVEKRPQISSRQRK KEKSELEEAFESANTVDEM+ AFKDMES FEERDL
Sbjct: 63  QANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEERDL 122

Query: 122 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLK 181
           GLASLKIGLKL+QE EDPEK L+YA+RALKALD DDN GK SL VAMTLQL+GSVNYSLK
Sbjct: 123 GLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDN-GKLSLPVAMTLQLLGSVNYSLK 182

Query: 182 KFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRK 241
           +FSDSLGYLNRANR+L QLEE GFS AE IRPVLHAVQLELANVKTAMGRREEAL NLRK
Sbjct: 183 RFSDSLGYLNRANRILGQLEESGFS-AEGIRPVLHAVQLELANVKTAMGRREEALANLRK 242

Query: 242 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAH 301
           CLEIKELTLEKDSRELGKA RDLAEAYVAILNF+DAL FCM+ALEIHK QLGHNSVEVAH
Sbjct: 243 CLEIKELTLEKDSRELGKAKRDLAEAYVAILNFKDALLFCMQALEIHKKQLGHNSVEVAH 302

Query: 302 DRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIS 361
           DRRLLGVIY+GL+EH+KALEQNELSQKVLKNWGLNSDLL AEIDAANMQIALGRYDEAIS
Sbjct: 303 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 362

Query: 362 TLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAE 421
           TLKDV Q T+KDSET+AVVFISMGKVL NQEKF DAKRCLEIA+ IFDKKEA SPVEAAE
Sbjct: 363 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 422

Query: 422 AYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAI 481
           AYSEISMQYE+MNEFETAISLLKRSLA+LEKLPQEQHS GSVSAR+GWLLLLTGKV EAI
Sbjct: 423 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSVGSVSARLGWLLLLTGKVHEAI 482

Query: 482 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 541
           PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH
Sbjct: 483 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 542

Query: 542 ADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAH 601
           ADSIE+CQNL+KAYG++GSYALAI+FQEQVVDAWE HGS+AEDEL+EAQRMLEQLKRKAH
Sbjct: 543 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 602

Query: 602 GTSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           G SK ELS KALPLPHS TSSK+LQP+IP SQ+
Sbjct: 603 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQE 633

BLAST of Moc09g09270 vs. ExPASy TrEMBL
Match: A0A0A0K8P3 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 PE=4 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 5.8e-291
Identity = 536/632 (84.81%), Postives = 579/632 (91.61%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSLISDPPPSPPPS-SSPLSAAHLNPCSKSHGLFFKLTRFQ 61
           R ASISLL + NRLNLRS+SSLIS    S  PS SSP+S  +L PC+ ++GLFFK TRFQ
Sbjct: 14  RNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSSPVSTPNLKPCTINNGLFFKFTRFQ 73

Query: 62  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG 121
           ANP RNLDTLVEKRP+ISSRQRK+K+KS LEE+FESA TVDEM  AFK+MESAFEE+DLG
Sbjct: 74  ANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEKDLG 133

Query: 122 LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK 181
           LASLKI LKLD+EGEDPE+ LLYA RALKALD DDN   PSL VAMTLQL+GSVNYSLK+
Sbjct: 134 LASLKIALKLDREGEDPEQVLLYADRALKALDKDDN-RNPSLPVAMTLQLIGSVNYSLKR 193

Query: 182 FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC 241
           FSDSLGYLNRANR+L QLEEKG+S AEDIRPVLHAV LELANVKTAMGRREEAL NLRKC
Sbjct: 194 FSDSLGYLNRANRILGQLEEKGYS-AEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 253

Query: 242 LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD 301
           LEIKEL LEKDSRELG ANRDLAEAYVAILNF+DALPFCMK LEIHK +LG+NSVEVAHD
Sbjct: 254 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 313

Query: 302 RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST 361
           RRLLGVIY+GL+E++KALEQNELS+KVLKNWGLNSDLL AE DAANMQIALGRYDEAI+T
Sbjct: 314 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 373

Query: 362 LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA 421
           LKDVAQ TDKDSE R VVF SMGKVLCNQEKFTDAKRCLEIASGIFDK+E TSPVEAAEA
Sbjct: 374 LKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 433

Query: 422 YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP 481
           YSEISMQYETMNEF+TAISLLK+SLA+LEKLPQEQHSEGSVSAR+GWLLLLTGKVQEAIP
Sbjct: 434 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 493

Query: 482 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA 541
           YLEGAAETLKESFGS HFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHHA
Sbjct: 494 YLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 553

Query: 542 DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG 601
           DSIE+C NLSKAYGAMGSYALAI+FQEQVVDAW+SHGSSAEDEL+EA+R LEQLKRKA G
Sbjct: 554 DSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKARG 613

Query: 602 TSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           TSK E STKALPLP S TSSK+LQP++P  Q+
Sbjct: 614 TSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQ 643

BLAST of Moc09g09270 vs. ExPASy TrEMBL
Match: A0A1S3BR15 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.3e-290
Identity = 533/632 (84.34%), Postives = 580/632 (91.77%), Query Frame = 0

Query: 2   RKASISLLSHSNRLNLRSISSLISDPPPSP-PPSSSPLSAAHLNPCSKSHGLFFKLTRFQ 61
           R ASIS+L + NRLNLRS+SSLIS    S  P +SSP+S  +L PC+K++GLFFK TRFQ
Sbjct: 3   RNASISILPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTRFQ 62

Query: 62  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEERDLG 121
           AN  RNLDTLVEKRP++SSRQRK+KEKS+LEEAFESA+TVDEM+ AFK+MESAFEE+DLG
Sbjct: 63  ANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKDLG 122

Query: 122 LASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYSLKK 181
           LASLKIGLKLDQEGEDPE+ LLYA RALKALD DDN   PSL VAMTLQL+GSVNYSLK+
Sbjct: 123 LASLKIGLKLDQEGEDPEQILLYADRALKALDKDDN-RNPSLPVAMTLQLIGSVNYSLKR 182

Query: 182 FSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNLRKC 241
           FSDSLGYLNRANR+L QLEEKGFS AED+RPVLHAVQLELANVKTAMGRREEAL NLRK 
Sbjct: 183 FSDSLGYLNRANRILGQLEEKGFS-AEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 242

Query: 242 LEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEVAHD 301
           LEIKEL LEKDSRELG ANRDLAEAYVAILNF+DALPFCMK LEIHK +LG+NSVEVAHD
Sbjct: 243 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 302

Query: 302 RRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEAIST 361
           RRLLGVIY+GL+EH+KALEQNELS+KVLKNWGLNSDLL AEI+AANMQIALGRYDEAIST
Sbjct: 303 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 362

Query: 362 LKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEAAEA 421
           LKDV Q TDKDSE R VVF SMGKVLCNQEKFTD KRCLEIA GIFDK+E TSP  AAEA
Sbjct: 363 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 422

Query: 422 YSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQEAIP 481
           YSEISM YETMN+FETAISLLKRSLA+LEKLPQEQHSEGSVSAR+GWLLLL GKVQEAIP
Sbjct: 423 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 482

Query: 482 YLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHHA 541
           YLEGAAETL+ESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHHA
Sbjct: 483 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 542

Query: 542 DSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRKAHG 601
           DSIE+C NLSKAYGAMGSYALAI+FQ+QVVDAWESHGS+AEDEL+EA++ L+QLKRKA G
Sbjct: 543 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 602

Query: 602 TSKPELSTKALPLPHSPTSSKSLQPNIPGSQK 633
           TSK ELSTKALPLPHS TSSK+LQP++P  QK
Sbjct: 603 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQK 632

BLAST of Moc09g09270 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 761.1 bits (1964), Expect = 6.9e-220
Identity = 403/624 (64.58%), Postives = 499/624 (79.97%), Query Frame = 0

Query: 1   MRKASISLLSHSNRLNLRSIS-SLISDPPPSPPPSSSPLSAAH--LNPCSKSHGLFFKLT 60
           M+  SISL+   +   +R I  S+  +   S   SSS +S A      C +++ L  +  
Sbjct: 1   MKSLSISLIRRLHFHKIRPIPLSIHLNAASSSSSSSSLISPAREISTSCRRTNDLIKR-- 60

Query: 61  RFQANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMESAFEER 120
           +F  NP RNL+T VE+ P+ISS     KEK +LEEAFESANT DEM+  FK+ME +FE  
Sbjct: 61  QFWVNPYRNLNTHVEEPPEISSSD---KEKIDLEEAFESANTTDEMVRLFKEMELSFEGN 120

Query: 121 DLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQLMGSVNYS 180
           +LGL++LK+GL LD+EGEDPEK L YA +ALK+ D D  G KP+LLVAM  QLMGS NY 
Sbjct: 121 ELGLSALKLGLHLDREGEDPEKVLSYADKALKSFDGD--GNKPNLLVAMASQLMGSANYG 180

Query: 181 LKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEALGNL 240
           LK+FSDSLGYLNRANR+L +LE  G    ED+RPVLHAVQLELANVK AMGRREEA+ NL
Sbjct: 181 LKRFSDSLGYLNRANRILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIENL 240

Query: 241 RKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQLGHNSVEV 300
           +K LEIKE+T ++DS+E+G ANR LA+AYVA+LNF +ALP+ +KALEIHK +LG+NS EV
Sbjct: 241 KKSLEIKEMTFDEDSKEMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSAEV 300

Query: 301 AHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIALGRYDEA 360
           A DRRLLGVIY+GL++H+KALEQN LSQ+VLKNWG+  +L+ AEIDAANM++ALG+Y+EA
Sbjct: 301 AQDRRLLGVIYSGLEQHDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYEEA 360

Query: 361 ISTLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKKEATSPVEA 420
           I  LK V Q+TDKDSE RA+VFISM K L NQ+KF ++KRCLE A  I +KKE   PVE 
Sbjct: 361 IDILKSVVQQTDKDSEMRAMVFISMSKALVNQQKFAESKRCLEFACEILEKKETALPVEV 420

Query: 421 AEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIGWLLLLTGKVQE 480
           AEAYSE++MQYE+MNEFETAISLL+++L +LEKLPQEQHSEGSVSARIGWLLL +G+V +
Sbjct: 421 AEAYSEVAMQYESMNEFETAISLLQKTLGILEKLPQEQHSEGSVSARIGWLLLFSGRVSQ 480

Query: 481 AIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGP 540
           A+PYLE AAE LKESFG+KHFGVGY+YNNLGAAYLEL RPQSAAQMFA+AKDIMDVSLGP
Sbjct: 481 AVPYLESAAERLKESFGAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDVSLGP 540

Query: 541 HHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELKEAQRMLEQLKRK 600
           +H DSI++CQNLSKAY  MG+Y+LA++FQ++V++AW++HG SA+DE+KEA+R+LE L+ K
Sbjct: 541 NHVDSIDACQNLSKAYAGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLEDLRLK 600

Query: 601 AH-GTSKPELSTKALPLPHSPTSS 621
           A  G S  +L  KALPLP    SS
Sbjct: 601 ARGGVSTNKLLNKALPLPKPSHSS 617

BLAST of Moc09g09270 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-45
Identity = 153/554 (27.62%), Postives = 265/554 (47.83%), Query Frame = 0

Query: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMES-AFEERDL 120
           +NP R   T ++K P  SS   + K         + + TV    +A  D++  + +  DL
Sbjct: 30  SNPPR---TPMKKTP--SSTPSRSKPSPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDL 89

Query: 121 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDN----------DDNGGKPSLLVAMTLQ 180
           G   LK+       GE P K L YA RA K+ +             + G P L +AM+L 
Sbjct: 90  GPFLLKLARDAIASGEGPNKALDYAIRATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLH 149

Query: 181 LMGSVNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGR 240
           ++ ++  SL +F +++  L RA +V         S A        +  ++L +  + +G+
Sbjct: 150 VLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHSLA------AFSGHMQLGDTLSMLGQ 209

Query: 241 REEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQ 300
            + ++    + L+I+  TL      +G+  R LAEAYV  + F  A   C K LEIH+  
Sbjct: 210 IDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAH 269

Query: 301 LGHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQI 360
               S+E A DRRL+ +I     ++E ALE   L+   +   G  S++   ++   N+ +
Sbjct: 270 SEPASLEEAADRRLMAIICEAKGDYENALEHLVLASMAMIASGQESEVASIDVSIGNIYM 329

Query: 361 ALGRYDEAI----STLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGI 420
           +L R+DEA+      L        +   T A VF+ + ++     K  ++K   E A  I
Sbjct: 330 SLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGKLRESKSYCENALRI 389

Query: 421 FDKK-EATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSAR 480
           ++K    T+  E A   +EIS  YE+++E E A+ LL++S+ +LE  P +Q +   + AR
Sbjct: 390 YNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKSMKLLEDKPGQQSAIAGLEAR 449

Query: 481 IGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMF 540
           +G +    G+ ++A    E A   L+ + G K    G + N +G A ++L +   A ++F
Sbjct: 450 MGVMYYTVGRYEDARNAFESAVTKLRAA-GEKSAFFGVVLNQMGLACVQLFKIDEAGELF 509

Query: 541 AMAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDEL 599
             A+ I++   GP   D++    NL+  Y AMG    AI+  EQV+   E    +A  + 
Sbjct: 510 EEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIEDAIEILEQVLKLREEKLGTANPDF 569

BLAST of Moc09g09270 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 8.5e-45
Identity = 145/520 (27.88%), Postives = 244/520 (46.92%), Query Frame = 0

Query: 115 EERDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKP-SLLVAMTLQLMGS 174
           E  +LG+  LK   +L   GE+  K L  A RA+K  +    G K   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 175 VNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRREEA 234
           +   L +++D++  L R+      +E       ED      A  ++L ++   MG+ E +
Sbjct: 209 IYAGLGRYNDAVPVLERS------IEIPMIEDGEDHALAKFAGCMQLGDMYGLMGQVENS 268

Query: 235 LGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQ--LG 294
           +      LEI+   L +    +G+  R LAEA+V  + F +A   C  AL+IHK      
Sbjct: 269 IMLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAA 328

Query: 295 HNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIAL 354
             S+E A DR+L+G+I     ++E ALE   L+   + +     D+   +    +  ++L
Sbjct: 329 TASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSL 388

Query: 355 GRYDEAI----STLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGIFD 414
            R+DEAI      L    Q   +   + A+V++ +  +     K  D+K   E A  I+ 
Sbjct: 389 ARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYL 448

Query: 415 KKEATSPV-EAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARIG 474
           K    +P+ E A  + EIS  Y++MNE + A+ LL+R+L +    P +Q++   + A++G
Sbjct: 449 KPTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMG 508

Query: 475 WLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAM 534
            +  + G   E+    + A    + S   K    G   N +G A ++      AA +F  
Sbjct: 509 VVTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEE 568

Query: 535 AKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELK- 594
           AK I++   GP+H D++    NL+  Y AMG    AI+  E VV   E    +A  E++ 
Sbjct: 569 AKTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVED 628

Query: 595 EAQRMLEQLKRKAHGTSKPELSTKALPLPHSPTSSKSLQP 626
           E QR+   LK    G SK   +   L L ++P  +   +P
Sbjct: 629 EKQRLAALLKEAGRGRSKRNRALLTL-LDNNPEIANGQRP 661

BLAST of Moc09g09270 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.8e-42
Identity = 143/513 (27.88%), Postives = 244/513 (47.56%), Query Frame = 0

Query: 61  ANPCRNLDTLVEKRPQISSRQRKIKEKSELEEAFESANTVDEMLSAFKDMES-AFEERDL 120
           +NP R   T ++K P  SS   + K         + + TV    +A  D++  + +  DL
Sbjct: 30  SNPPR---TPMKKTP--SSTPSRSKPSPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDL 89

Query: 121 GLASLKIGLKLDQEGEDPEKTLLYASRALKALDN----------DDNGGKPSLLVAMTLQ 180
           G   LK+       GE P K L YA RA K+ +             + G P L +AM+L 
Sbjct: 90  GPFLLKLARDAIASGEGPNKALDYAIRATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLH 149

Query: 181 LMGSVNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGR 240
           ++ ++  SL +F +++  L RA +V         S A        +  ++L +  + +G+
Sbjct: 150 VLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHSLA------AFSGHMQLGDTLSMLGQ 209

Query: 241 REEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQ 300
            + ++    + L+I+  TL      +G+  R LAEAYV  + F  A   C K LEIH+  
Sbjct: 210 IDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAH 269

Query: 301 LGHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQI 360
               S+E A DRRL+ +I     ++E ALE   L+   +   G  S++   ++   N+ +
Sbjct: 270 SEPASLEEAADRRLMAIICEAKGDYENALEHLVLASMAMIASGQESEVASIDVSIGNIYM 329

Query: 361 ALGRYDEAI----STLKDVAQRTDKDSETRAVVFISMGKVLCNQEKFTDAKRCLEIASGI 420
           +L R+DEA+      L        +   T A VF+ + ++     K  ++K   E A  I
Sbjct: 330 SLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGKLRESKSYCENALRI 389

Query: 421 FDKK-EATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSAR 480
           ++K    T+  E A   +EIS  YE+++E E A+ LL++S+ +LE  P +Q +   + AR
Sbjct: 390 YNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKSMKLLEDKPGQQSAIAGLEAR 449

Query: 481 IGWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMF 540
           +G +    G+ ++A    E A   L+ + G K    G + N +G A ++L +   A ++F
Sbjct: 450 MGVMYYTVGRYEDARNAFESAVTKLRAA-GEKSAFFGVVLNQMGLACVQLFKIDEAGELF 509

Query: 541 AMAKDIMDVSLGPHHADSIESCQNLSKAYGAMG 558
             A+ I++   GP   D++    NL+  Y AMG
Sbjct: 510 EEARGILEQERGPCDQDTLGVYSNLAATYDAMG 530

BLAST of Moc09g09270 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 1.2e-41
Identity = 141/519 (27.17%), Postives = 243/519 (46.82%), Query Frame = 0

Query: 111 ESAFEERDLGLASLKIGLKLDQEGEDPEKTLLYASRALKALDNDDNGGKPSLLVAMTLQL 170
           E + E  +L    L     L   G+   K L    RA K  +     GKP L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 171 MGSVNYSLKKFSDSLGYLNRANRVLSQLEEKGFSAAEDIRPVLHAVQLELANVKTAMGRR 230
             +V+  LK++++++  L R+  +   + E+G    E+      A  ++L +    +G+ 
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEI--PVVEEG----EEHALAKFAGLMQLGDTYAMVGQL 257

Query: 231 EEALGNLRKCLEIKELTLEKDSRELGKANRDLAEAYVAILNFRDALPFCMKALEIHKMQL 290
           E ++    + L I++  L ++   +G+  R LAEA V  L F +A   C  AL IH+   
Sbjct: 258 ESSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESG 317

Query: 291 GHNSVEVAHDRRLLGVIYTGLDEHEKALEQNELSQKVLKNWGLNSDLLCAEIDAANMQIA 350
              S+  A DRRL+G+I     +HE ALE   L+   +   G  S++   +    +  ++
Sbjct: 318 LPGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLS 377

Query: 351 LGRYDEAISTLKD--VAQRTDKDSETRAV--VFISMGKVLCNQEKFTDAKRCLEIASGIF 410
           L R+DEAI   +    A +T K     AV  V+I +  +     K  +AK   E A  I+
Sbjct: 378 LSRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIY 437

Query: 411 DKKE-ATSPVEAAEAYSEISMQYETMNEFETAISLLKRSLAVLEKLPQEQHSEGSVSARI 470
           +      SP E A   ++IS+  E+MNE E AI+LL+++L +    P ++     + A++
Sbjct: 438 ESHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQM 497

Query: 471 GWLLLLTGKVQEAIPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFA 530
           G L  + GK  E+    + A   L+ +   +    G   N +G A ++L+  + A ++F 
Sbjct: 498 GVLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFE 557

Query: 531 MAKDIMDVSLGPHHADSIESCQNLSKAYGAMGSYALAIDFQEQVVDAWESHGSSAEDELK 590
            AK I++   GP+H +++    NL+ AY A+G    AI     VV   E    +A    +
Sbjct: 558 EAKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTE 617

Query: 591 EAQRMLEQLKRKAHGTSKPELSTKALPLPHSPTSSKSLQ 625
           + +R L QL ++A   +  +  +    +    TSS +L+
Sbjct: 618 DEKRRLAQLLKEAGNVTGRKAKSLKTLIDSDLTSSSALR 650

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143612.10.0e+00100.00protein KINESIN LIGHT CHAIN-RELATED 1 [Momordica charantia][more]
XP_038899312.11.4e-30788.49protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida][more]
XP_022954096.14.2e-30487.89protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita moschata][more]
XP_023549125.11.6e-30387.58protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo][more]
KAG7014584.11.8e-30287.26Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
O816291.9e-4427.62Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 P... [more]
Q9LII81.2e-4327.88Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 P... [more]
F4HSX91.6e-4027.17Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 P... [more]
A0JM236.2e-1622.38Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2[more]
Q6AZT71.4e-1522.10Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CRC10.0e+00100.00protein KINESIN LIGHT CHAIN-RELATED 1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1GPX72.0e-30487.89protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JWL05.7e-29987.05protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC11148813... [more]
A0A0A0K8P35.8e-29184.81TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 ... [more]
A0A1S3BR151.3e-29084.34nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G31240.16.9e-22064.58Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.11.3e-4527.62Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.18.5e-4527.88Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.21.8e-4227.88Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G27500.11.2e-4127.17Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 431..451
NoneNo IPR availableCOILSCoilCoilcoord: 577..597
NoneNo IPR availablePFAMPF13424TPR_12coord: 468..530
e-value: 4.0E-9
score: 36.6
NoneNo IPR availablePFAMPF13374TPR_10coord: 217..253
e-value: 0.0013
score: 18.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 594..634
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 612..634
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..42
NoneNo IPR availablePANTHERPTHR47459KINESIN LIGHT CHAIN-RELATEDcoord: 1..622
NoneNo IPR availablePANTHERPTHR47459:SF1KINESIN LIGHT CHAIN-RELATEDcoord: 1..622
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 543..576
e-value: 62.0
score: 8.0
coord: 256..289
e-value: 37.0
score: 9.9
coord: 165..198
e-value: 220.0
score: 3.2
coord: 501..534
e-value: 31.0
score: 10.6
coord: 376..409
e-value: 35.0
score: 10.2
coord: 214..247
e-value: 25.0
score: 11.4
coord: 459..492
e-value: 330.0
score: 1.6
coord: 418..451
e-value: 15.0
score: 13.5
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 544..571
e-value: 0.045
score: 14.0
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 418..451
score: 8.2309
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 248..412
e-value: 4.0E-13
score: 51.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 108..247
e-value: 2.0E-5
score: 26.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 415..611
e-value: 7.9E-27
score: 96.0
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 416..564
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 214..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g09270.1Moc09g09270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding