Homology
BLAST of Moc09g09260 vs. NCBI nr
Match:
XP_022143610.1 (hyoscyamine 6-dioxygenase-like [Momordica charantia])
HSP 1 Score: 710.3 bits (1832), Expect = 8.0e-201
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0
Query: 1 MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60
MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA
Sbjct: 1 MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60
Query: 61 SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120
SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK
Sbjct: 61 SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120
Query: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180
EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180
Query: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240
KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240
Query: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300
VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300
Query: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340
BLAST of Moc09g09260 vs. NCBI nr
Match:
XP_038897058.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])
HSP 1 Score: 604.4 bits (1557), Expect = 6.2e-169
Identity = 280/340 (82.35%), Postives = 308/340 (90.59%), Query Frame = 0
Query: 1 MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60
MGD FV+NW GV+SVPESYVYPP KRPGNV VP+ENAIPV+DL+ DR L+ KILDA
Sbjct: 1 MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60
Query: 61 SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120
S+EFGFFQVINHGVSKT+ ETMR+F+EFHAMSGPEK +ECSKDPNRSCRVYTSSEN++K
Sbjct: 61 SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120
Query: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180
E+ HCWRDALIFNCHPL+KYVHFWPQNP YREVVGAYC+ MRKLV+EILEL+SEGLGLG
Sbjct: 121 EQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLG 180
Query: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240
KGYFG EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDG+WIG
Sbjct: 181 KGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIG 240
Query: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300
V P+DNAFVVNIGFVLQV++NGKLKA EHRAV NAK SRQS+TYLVYPKD+ATMEPAKCM
Sbjct: 241 VQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCM 300
Query: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
INEANP Y SL FKDFQRNYLP A DTKAV++YI S+ S
Sbjct: 301 INEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 340
BLAST of Moc09g09260 vs. NCBI nr
Match:
XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 591.3 bits (1523), Expect = 5.4e-165
Identity = 271/337 (80.42%), Postives = 304/337 (90.21%), Query Frame = 0
Query: 4 QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63
+V FVSNW GV+SVP YVYPPEKRPGNV VP+ N IPV+DL+ DR LV KILDASQE
Sbjct: 3 EVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQE 62
Query: 64 FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 123
FGFFQVINHGVSK ++EET R+F++FHAMSG EK +ECS+DPN+SCRVYTSSEN+ KE+T
Sbjct: 63 FGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQT 122
Query: 124 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 183
HCWRDALIFNCHPLDKYVHFWPQNP YREVVGAYC+ MR LV+EILEL+SEGLGLG+GY
Sbjct: 123 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGY 182
Query: 184 FGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHP 243
G EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ++NGLQVLKDGQWIGVHP
Sbjct: 183 LGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHP 242
Query: 244 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 303
+DNAFVVNIGFVLQV+SNGKLKA EHRAV NAK+SRQS+TYLVYPKD+ TMEPAKC+INE
Sbjct: 243 IDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINE 302
Query: 304 ANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
ANP Y +L+FKDFQRNYLP A+DTKAV++ I SD S
Sbjct: 303 ANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
BLAST of Moc09g09260 vs. NCBI nr
Match:
XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])
HSP 1 Score: 590.5 bits (1521), Expect = 9.2e-165
Identity = 272/334 (81.44%), Postives = 300/334 (89.82%), Query Frame = 0
Query: 7 FVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGF 66
FVSNW VESVPESYVYPPEKRPGN+ VP+ AIPV+DL+ DR LV KILDAS+EFGF
Sbjct: 8 FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67
Query: 67 FQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCW 126
FQ+INHGVSK + EETMR+F+EFHAMSGPEK +ECSKDPNRSCRVYTSSEN+ KE+ HCW
Sbjct: 68 FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127
Query: 127 RDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGD 186
RDALIFNCHPL+KYVHFWPQ P YREVVGAYC+ MRKLV+EILEL+SEGLGLGKGYFG
Sbjct: 128 RDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGG 187
Query: 187 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHPVDN 246
EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDGQWIGV P+DN
Sbjct: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDN 247
Query: 247 AFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEANP 306
AFVVNIGFVLQ+++NGKL+A EHRAV N+K SRQS+TYLVYPKD+A MEPAKCMINEANP
Sbjct: 248 AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANP 307
Query: 307 SLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
Y SL FKDFQRNYLP A DTKAV+QYI ++ S
Sbjct: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of Moc09g09260 vs. NCBI nr
Match:
KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 589.7 bits (1519), Expect = 1.6e-164
Identity = 270/335 (80.60%), Postives = 303/335 (90.45%), Query Frame = 0
Query: 4 QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63
+V FVSNW GV+SVP YVYPPEKRPGNV VP+ NAIPV+DL+ DR LV KILDASQE
Sbjct: 3 EVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQE 62
Query: 64 FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 123
FGFFQVINHGVS+ ++EET R+F++FHAMSG EK +ECS+DPN+SCRVYTSSEN+ KE+T
Sbjct: 63 FGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQT 122
Query: 124 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 183
HCWRDALIFNCHPLDKYVHFWPQNP YREVVGAYCI MR LV+EILEL+SEGLGLG+GY
Sbjct: 123 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGY 182
Query: 184 FGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHP 243
G EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ++NGLQVLKDGQWIGVHP
Sbjct: 183 LGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHP 242
Query: 244 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 303
+DNAFVVNIGFVLQV+SNGKLKA EHR V NAK+SRQS+TYLVYPKD+ TMEPAKC+INE
Sbjct: 243 IDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINE 302
Query: 304 ANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSD 339
ANP Y +L+FKDFQRNYLP A+DTKAV++ I SD
Sbjct: 303 ANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 337
BLAST of Moc09g09260 vs. ExPASy Swiss-Prot
Match:
P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)
HSP 1 Score: 266.5 bits (680), Expect = 4.0e-70
Identity = 143/332 (43.07%), Postives = 198/332 (59.64%), Query Frame = 0
Query: 7 FVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGF 66
FVSNW +SV ES++ P +KR VPV N +P++DL H LV +I A Q+FG
Sbjct: 4 FVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDFGL 63
Query: 67 FQVINHGVSKTLIEETMRVFEEFHAMSGPEK-----KRECSK---DPNRSCRVYTSSENF 126
FQVINHG + L+ ETM V +EF A+ EK K E +K + ++Y E
Sbjct: 64 FQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQL 123
Query: 127 VKEKTHCWRDALIFNCHPLDK-YVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGL 186
E+ W+D L CHPLD+ V+ WP+ P+ YREVV Y + +RKL + +L+ I EGL
Sbjct: 124 SNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGL 183
Query: 187 GLGKGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQ--VLKD 246
GL GYF +E+S+ ++L N+YPPCP+PS TLG H D +LIT+L Q + GLQ ++KD
Sbjct: 184 GLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKD 243
Query: 247 GQWIGVHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATME 306
WI V P+ AFVVN+G L+V++N K + HR V + R SI L+ P T+E
Sbjct: 244 ATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIE 303
Query: 307 PAKCMINEANPSLYHSLQFKDFQRNYLPMAAD 328
PAK ++N+ NP LY + +F YL +D
Sbjct: 304 PAKELLNQDNPPLYKPYSYSEFADIYLSDKSD 332
BLAST of Moc09g09260 vs. ExPASy Swiss-Prot
Match:
Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)
HSP 1 Score: 251.1 bits (640), Expect = 1.7e-65
Identity = 127/310 (40.97%), Postives = 183/310 (59.03%), Query Frame = 0
Query: 15 ESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGFFQVINHGV 74
+++P YV P +RP V + IPVVDL DRA +V + DA + GFFQV+NHG+
Sbjct: 18 DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77
Query: 75 SKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCWRDALIFNC 134
LI M V EF + EK + S DP + R+ TS N KE H WRD L +C
Sbjct: 78 DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137
Query: 135 HPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGDEMSENPL- 194
+PL ++V WP NP +++E++G YC +R+L + E ISE LGL GY + + E
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197
Query: 195 LLVNHYPPCPNPSLTLGLSQHCDPSLITILF--QQINGLQVLKDGQWIGVHPVDNAFVVN 254
+ VN+YP CP P LT GL H DP+ +TIL Q+ GLQVL DG+WI V+P A V+N
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257
Query: 255 IGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEANPSLYHS 314
IG LQ +SNGK ++ HRAV+N+ R S+ + P + + PAK +I + +P++Y +
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317
Query: 315 LQFKDFQRNY 322
+ ++ + +
Sbjct: 318 YTYDEYYKKF 326
BLAST of Moc09g09260 vs. ExPASy Swiss-Prot
Match:
Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)
HSP 1 Score: 247.3 bits (630), Expect = 2.5e-64
Identity = 131/286 (45.80%), Postives = 178/286 (62.24%), Query Frame = 0
Query: 16 SVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGFFQVINHGVS 75
++PE+YV P RP V P++DL+ DR+FL+ +I A FGFFQVINHGV+
Sbjct: 14 TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73
Query: 76 KTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCWRDALIFNCH 135
K +I+E + V EF +MS EK + S DP ++ R+ TS N KE+ + WRD L +C+
Sbjct: 74 KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133
Query: 136 PLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGDEMSE-NPLL 195
P+ KYV+ WP NP +++E+V Y +R++ +I ELISE LGL K Y + E +
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193
Query: 196 LVNHYPPCPNPSLTLGLSQHCDPSLITILFQQ--INGLQVLKDGQWIGVHPVDNAFVVNI 255
VN+YPPCP P LT GL H DP+ +TIL Q + GLQ+L DGQW V+P +AFV+NI
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253
Query: 256 GFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAK 299
G LQ +SNG K+ HRAV N + R S+ + P D A M PAK
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAK 298
BLAST of Moc09g09260 vs. ExPASy Swiss-Prot
Match:
Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)
HSP 1 Score: 239.6 bits (610), Expect = 5.2e-62
Identity = 128/327 (39.14%), Postives = 195/327 (59.63%), Query Frame = 0
Query: 14 VESVPESYVYPPEKRPGNVAVPVE-NAIPVVDLTD---HDRAFLVHKILDASQEFGFFQV 73
V+ VP +YV P RP V ++IP++DL D +RA ++++ A GFFQ+
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74
Query: 74 INHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCWRDA 133
NHGV + I++ M EF S E+ + S D ++ R+ TS N KEK WRD
Sbjct: 75 KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134
Query: 134 LIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGDEMS 193
L +C+P++ +++ WP P ++REV Y +R LV+ +LE ISE LGL K + +
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194
Query: 194 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-QINGLQVLKDGQWIGVHPVDNA 253
++ + +N+YP CP P LT GL H D +LIT+L Q +++GLQV KDG+WI V+PV N
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254
Query: 254 FVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEA--N 313
F+VN+G +QV+SN K K+ HRAV+N+ M R SI P +DA + PA+ +INE +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314
Query: 314 PSLYHSLQFKDFQRNYLPMAADTKAVI 333
P++Y + + ++ + A DT++ I
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTESCI 340
BLAST of Moc09g09260 vs. ExPASy Swiss-Prot
Match:
Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)
HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 120/317 (37.85%), Postives = 184/317 (58.04%), Query Frame = 0
Query: 8 VSNWVGVESVPESYVYPPEKRPGNVAVPVE-NAIPVVDLTD---HDRAFLVHKILDASQE 67
VS++ +P +YV P RP V ++IP++DL D +RA +V ++ A
Sbjct: 11 VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70
Query: 68 FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 127
+GFFQ+ NHGV T + + V EF E+ + S DP ++ R+ TS N +K
Sbjct: 71 YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130
Query: 128 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 187
WRD L +C P++ ++ WP +P ++REV Y +R LV+ +LE ISE LGL +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190
Query: 188 FGDEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-QINGLQVLKDGQWIGV 247
+ + ++ + N+YPPCP P LT GL H DP++IT+L Q Q++GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250
Query: 248 HPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMI 307
P+ N F+VNIG +QV+SN K K+ HRAV+N + R SI +P DA + PA ++
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310
Query: 308 NEANP-SLYHSLQFKDF 318
NE + ++Y + F ++
Sbjct: 311 NEQDSLAIYRTYPFVEY 326
BLAST of Moc09g09260 vs. ExPASy TrEMBL
Match:
A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)
HSP 1 Score: 710.3 bits (1832), Expect = 3.9e-201
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0
Query: 1 MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60
MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA
Sbjct: 1 MGDQVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDA 60
Query: 61 SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120
SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK
Sbjct: 61 SQEFGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVK 120
Query: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180
EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG
Sbjct: 121 EKTHCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLG 180
Query: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240
KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG
Sbjct: 181 KGYFGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIG 240
Query: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300
VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM
Sbjct: 241 VHPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCM 300
Query: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS
Sbjct: 301 INEANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340
BLAST of Moc09g09260 vs. ExPASy TrEMBL
Match:
A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)
HSP 1 Score: 590.5 bits (1521), Expect = 4.5e-165
Identity = 272/334 (81.44%), Postives = 300/334 (89.82%), Query Frame = 0
Query: 7 FVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGF 66
FVSNW VESVPESYVYPPEKRPGN+ VP+ AIPV+DL+ DR LV KILDAS+EFGF
Sbjct: 8 FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67
Query: 67 FQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCW 126
FQ+INHGVSK + EETMR+F+EFHAMSGPEK +ECSKDPNRSCRVYTSSEN+ KE+ HCW
Sbjct: 68 FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127
Query: 127 RDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGD 186
RDALIFNCHPL+KYVHFWPQ P YREVVGAYC+ MRKLV+EILEL+SEGLGLGKGYFG
Sbjct: 128 RDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGG 187
Query: 187 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHPVDN 246
EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDGQWIGV P+DN
Sbjct: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDN 247
Query: 247 AFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEANP 306
AFVVNIGFVLQ+++NGKL+A EHRAV N+K SRQS+TYLVYPKD+A MEPAKCMINEANP
Sbjct: 248 AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANP 307
Query: 307 SLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
Y SL FKDFQRNYLP A DTKAV+QYI ++ S
Sbjct: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of Moc09g09260 vs. ExPASy TrEMBL
Match:
A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)
HSP 1 Score: 589.7 bits (1519), Expect = 7.6e-165
Identity = 270/335 (80.60%), Postives = 303/335 (90.45%), Query Frame = 0
Query: 4 QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63
+V FVSNW GV+SVP YVYPPEKRPGNV VP+ NAIPV+DL+ HDR LV KILDASQE
Sbjct: 3 EVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQE 62
Query: 64 FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 123
FGFFQVINHGVS+ ++EET R+F++FHAMSG EK +ECS+DPN+SCRVYTSSEN+ KE+T
Sbjct: 63 FGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQT 122
Query: 124 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 183
HCWRDALIFNCHPLDKYVHFWPQNP YREVVGAYCI MR LV+EILEL+SEGLGLG+GY
Sbjct: 123 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGY 182
Query: 184 FGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHP 243
G EMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ++NGLQVLKDGQWIGVHP
Sbjct: 183 LGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHP 242
Query: 244 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 303
+DNAFVVNIGFVLQV+SNGKLKA EHR V NAK+SRQS+TYLVYPKD+ TMEPAKC+INE
Sbjct: 243 IDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLINE 302
Query: 304 ANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSD 339
ANP Y +L+FKDFQRNYLP A+DTKAV++ I SD
Sbjct: 303 ANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 337
BLAST of Moc09g09260 vs. ExPASy TrEMBL
Match:
A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)
HSP 1 Score: 583.6 bits (1503), Expect = 5.5e-163
Identity = 267/337 (79.23%), Postives = 303/337 (89.91%), Query Frame = 0
Query: 4 QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63
+V FVSNW GV+SVP Y+YPPEKRPGNV VP+ NAIPVVDL+ DR LV KILDASQE
Sbjct: 3 EVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDASQE 62
Query: 64 FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 123
FGFFQVINHGVS+ ++EET R+F++FHAMSG EK +ECS+DP +SCRVYTSSEN+ KE+T
Sbjct: 63 FGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKEQT 122
Query: 124 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 183
HCWRDALIFNCHPL+KYVHFWPQNP YREVVGAYC+ MR LV+EILEL+SEGLGLG+GY
Sbjct: 123 HCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGRGY 182
Query: 184 FGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHP 243
EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ++NGLQVLKDGQWIGVHP
Sbjct: 183 LASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHP 242
Query: 244 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 303
+DNAFVVNIGFVLQV+SNGKLKA EHRAV NAK+SRQS+TYLVYPKD+ TMEPAKC+INE
Sbjct: 243 IDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINE 302
Query: 304 ANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
ANP Y +L+FKDFQRNYLP A+DTKAV++ I S+ S
Sbjct: 303 ANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339
BLAST of Moc09g09260 vs. ExPASy TrEMBL
Match:
A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)
HSP 1 Score: 581.3 bits (1497), Expect = 2.7e-162
Identity = 270/334 (80.84%), Postives = 297/334 (88.92%), Query Frame = 0
Query: 7 FVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGF 66
FVSNW ESVPESYVYPPEKRPGNV VP+ AIPV+DL+ DRA LV KIL AS+E GF
Sbjct: 8 FVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASKESGF 67
Query: 67 FQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCW 126
FQ+INHGVSK + E TMR+F+EFHAMSGPEK +ECSKDPNRSCRVYTSSEN+ KE+ HCW
Sbjct: 68 FQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQIHCW 127
Query: 127 RDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGD 186
RDALIFNCHPL+KYVHFWPQNP YREVVGAYC+ MRKLV+EILEL+SEGLGLGKGY G
Sbjct: 128 RDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYLGG 187
Query: 187 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHPVDN 246
EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDGQWIGV P+D
Sbjct: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDK 247
Query: 247 AFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEANP 306
AFVVNIGFVLQ+++NGKL+A EHRAV N+K SRQS+TYLVYPKD+ATMEPAKCMINEANP
Sbjct: 248 AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMINEANP 307
Query: 307 SLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 341
Y SL FKDFQRNYLP A DTKAV+QYI ++ S
Sbjct: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
BLAST of Moc09g09260 vs. TAIR 10
Match:
AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 247.3 bits (630), Expect = 1.8e-65
Identity = 131/286 (45.80%), Postives = 178/286 (62.24%), Query Frame = 0
Query: 16 SVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGFFQVINHGVS 75
++PE+YV P RP V P++DL+ DR+FL+ +I A FGFFQVINHGV+
Sbjct: 14 TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73
Query: 76 KTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCWRDALIFNCH 135
K +I+E + V EF +MS EK + S DP ++ R+ TS N KE+ + WRD L +C+
Sbjct: 74 KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133
Query: 136 PLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGDEMSE-NPLL 195
P+ KYV+ WP NP +++E+V Y +R++ +I ELISE LGL K Y + E +
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193
Query: 196 LVNHYPPCPNPSLTLGLSQHCDPSLITILFQQ--INGLQVLKDGQWIGVHPVDNAFVVNI 255
VN+YPPCP P LT GL H DP+ +TIL Q + GLQ+L DGQW V+P +AFV+NI
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253
Query: 256 GFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAK 299
G LQ +SNG K+ HRAV N + R S+ + P D A M PAK
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAK 298
BLAST of Moc09g09260 vs. TAIR 10
Match:
AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 239.6 bits (610), Expect = 3.7e-63
Identity = 128/327 (39.14%), Postives = 195/327 (59.63%), Query Frame = 0
Query: 14 VESVPESYVYPPEKRPGNVAVPVE-NAIPVVDLTD---HDRAFLVHKILDASQEFGFFQV 73
V+ VP +YV P RP V ++IP++DL D +RA ++++ A GFFQ+
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74
Query: 74 INHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCWRDA 133
NHGV + I++ M EF S E+ + S D ++ R+ TS N KEK WRD
Sbjct: 75 KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134
Query: 134 LIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGDEMS 193
L +C+P++ +++ WP P ++REV Y +R LV+ +LE ISE LGL K + +
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194
Query: 194 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-QINGLQVLKDGQWIGVHPVDNA 253
++ + +N+YP CP P LT GL H D +LIT+L Q +++GLQV KDG+WI V+PV N
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254
Query: 254 FVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEA--N 313
F+VN+G +QV+SN K K+ HRAV+N+ M R SI P +DA + PA+ +INE +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314
Query: 314 PSLYHSLQFKDFQRNYLPMAADTKAVI 333
P++Y + + ++ + A DT++ I
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTESCI 340
BLAST of Moc09g09260 vs. TAIR 10
Match:
AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 120/317 (37.85%), Postives = 184/317 (58.04%), Query Frame = 0
Query: 8 VSNWVGVESVPESYVYPPEKRPGNVAVPVE-NAIPVVDLTD---HDRAFLVHKILDASQE 67
VS++ +P +YV P RP V ++IP++DL D +RA +V ++ A
Sbjct: 11 VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70
Query: 68 FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 127
+GFFQ+ NHGV T + + V EF E+ + S DP ++ R+ TS N +K
Sbjct: 71 YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130
Query: 128 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 187
WRD L +C P++ ++ WP +P ++REV Y +R LV+ +LE ISE LGL +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190
Query: 188 FGDEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-QINGLQVLKDGQWIGV 247
+ + ++ + N+YPPCP P LT GL H DP++IT+L Q Q++GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250
Query: 248 HPVDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMI 307
P+ N F+VNIG +QV+SN K K+ HRAV+N + R SI +P DA + PA ++
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310
Query: 308 NEANP-SLYHSLQFKDF 318
NE + ++Y + F ++
Sbjct: 311 NEQDSLAIYRTYPFVEY 326
BLAST of Moc09g09260 vs. TAIR 10
Match:
AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 200.3 bits (508), Expect = 2.5e-51
Identity = 113/318 (35.53%), Postives = 171/318 (53.77%), Query Frame = 0
Query: 13 GVESVPESYVYPPEKRP--GNVAVPVENAIPVVDLTDHDRAFL----VHKILDASQEFGF 72
GV VP+ YV PP +RP G+ E +PV+DL+ + FL +H+I A +EFGF
Sbjct: 23 GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82
Query: 73 FQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCW 132
FQVINHG+ +++ + + +F + EK S + + R Y +S N ++ H W
Sbjct: 83 FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142
Query: 133 RDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGD 192
RD + HPL K++ WP NP Y++ VG Y L +++E ISE LGL K Y +
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202
Query: 193 EMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLK-DGQWIGVHPV 252
E+ E + ++ VN YP CP P + LG+ H D S +TIL Q GLQ++ + W+ V +
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262
Query: 253 DNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEA 312
+ A +V +G ++V+SNG K+ HR +N ++ R S L + PA ++N
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322
Query: 313 NPSLYHSLQFKDFQRNYL 323
N Y F DF NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338
BLAST of Moc09g09260 vs. TAIR 10
Match:
AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 189.1 bits (479), Expect = 5.8e-48
Identity = 109/314 (34.71%), Postives = 162/314 (51.59%), Query Frame = 0
Query: 13 GVESVPESYVYPPEKRP---GNVAVPVENAIPVVDLTD-HD---RAFLVHKILDASQEFG 72
G VP Y+ PP +RP ++ N +PV+DL+ HD R ++H+I A + FG
Sbjct: 23 GDPHVPTRYILPPSQRPMLGPSIGTSTIN-LPVIDLSFLHDPLLRLCVIHEIELACKGFG 82
Query: 73 FFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHC 132
FFQVINHG+S ++++ F + EK S + R Y +S N ++ H
Sbjct: 83 FFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSINHSTDRVHY 142
Query: 133 WRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFG 192
WRD + HPL +++ WP NP Y+E VG Y L +++E ISE LGL K Y
Sbjct: 143 WRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQ 202
Query: 193 DEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLK-DGQWIGVHP 252
+E+ E + ++ VN YP CP P + LG+ H D +TIL Q GLQ+ + W+ V
Sbjct: 203 EEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDCNNNWVCVPY 262
Query: 253 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 312
++ A +V +G ++V+SNG K+ HR +N R S L + PA ++NE
Sbjct: 263 IEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNE 322
Query: 313 ANPSLYHSLQFKDF 318
P+ Y F DF
Sbjct: 323 NKPAAYGEFSFNDF 334
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022143610.1 | 8.0e-201 | 100.00 | hyoscyamine 6-dioxygenase-like [Momordica charantia] | [more] |
| XP_038897058.1 | 6.2e-169 | 82.35 | hyoscyamine 6-dioxygenase-like [Benincasa hispida] | [more] |
| XP_023514953.1 | 5.4e-165 | 80.42 | hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_004146985.1 | 9.2e-165 | 81.44 | hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa... | [more] |
| KAG6593078.1 | 1.6e-164 | 80.60 | Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| P24397 | 4.0e-70 | 43.07 | Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1 | [more] |
| Q8W2X5 | 1.7e-65 | 40.97 | Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... | [more] |
| Q9FLV0 | 2.5e-64 | 45.80 | Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... | [more] |
| Q9ZSA7 | 5.2e-62 | 39.14 | Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1 | [more] |
| Q9ZSA8 | 2.1e-58 | 37.85 | Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CP90 | 3.9e-201 | 100.00 | hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE... | [more] |
| A0A0A0K5H3 | 4.5e-165 | 81.44 | Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... | [more] |
| A0A6J1H5H6 | 7.6e-165 | 80.60 | hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=... | [more] |
| A0A6J1KW36 | 5.5e-163 | 79.23 | hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ... | [more] |
| A0A1S3BR20 | 2.7e-162 | 80.84 | hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G24530.1 | 1.8e-65 | 45.80 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10490.1 | 3.7e-63 | 39.14 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10500.1 | 1.5e-59 | 37.85 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT2G44800.1 | 2.5e-51 | 35.53 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT3G60290.1 | 5.8e-48 | 34.71 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
