Moc09g05900 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g05900
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRING-type E3 ubiquitin transferase
Locationchr9: 4564700 .. 4569701 (-)
RNA-Seq ExpressionMoc09g05900
SyntenyMoc09g05900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGATAGCTGTGCTGTGTGTGCCGATACTTTGGAATGGGTTGCCTATGGTTCTTGTGGGCATCGGGATGTCTGCTCCACCTGTGTTGCTCGACTTCGATTCATTTGCGGTGATCGCCGCTGTTGCATCTGCAAGTCGGAGTCCGCCGTCGTGTTCGTCACTAAGGTGAGGGTGCAGCTATCCCTGTTTTGCGATCCCATTCAATCTTTGGAGATATTTAATTGTGGAATTCTGGCCTTTGTTTTTCTTTCATATGCAATTTGGTTTTTTCCCAAGTGTTCTGCTGCTCTAGTTGTATTCGAATTCTTTATGGTTCCTCGTTTCATTTAAATTTTTGGAAATTATGAATAACTTTCTGTTTCTGTTCGATTTGGATTGTCATACTTACACCAACGGGATATCGTGCGCACTTGTAATGCCTGATTATTCATAAATTTTATGTACTTCAAGTATTTATATTGATGCAATCACATTTTTCAAAAAAGAAAAATGAAATGATAACAGAAAACTGTTATCTGAACAGTTGGCCAAGACACCACGAGTGGTGGCAACTGTGCATCAATAAGGTCGAGGAAAGGAAAGTTTGTGTACTTAGCTTGATCTCTCATTAACGTTTATATTCTATTATGATCATATATGTTCATAGTTGGTGTCCATAACACTGGTTGGACATTTTCCCAGCTACAATCATAATTATTGGTTACAGGCTTTCAGCCTTACCCGTTTTCTTATTTTTTGTGATTAATATTTCCAGCTGACTAGCTGAAATTAGGATTGTGGAGATGAAAGGAAGCCACCCCTTAGAGAAACAAATGAACTTAAAAAATTTAAGGTTGTCCTGTATGTTCGTAAGAGAAAAATTCTTTGTCCTAATGCACCAAATGCTAGATAGCTGCTGAAAGCACAATTCAAAAAACACAATACTTGCTCCAGCCACACCTTTGTAACTCCATTATCCGGATTTGTTACAATCCTTGATACTTAAACACCCCCAAATAAAGAGACAAGCAAGAACTTTCAGTTGTTATAACAGGGTTGAAATCTATTTGGTGCTTCTTCAAACATTACTTGATGGAGATTGATGACTGTTTCGTTCATGTTGGTTTATGCTGCAGGCCTTAGGAGATTATACAAGGACGATTAATGACTTTACAGTCTTTCCTTCTGAGCCAAGGGAAGGCCGAGCTGGATCCTATTGGTATCATGAAGACACACAAGCATTTTTCGATGATGTTGACCACTACAAGATGATAAAAGCAATGTGCAGGCTGTCATGCAGCGTCTGTGATAAGATAGGTGAAGATCAACCAAATGATGGACCAAAGCGGCGAGGAAAATTTCGGAACATCGAGCAGTTAAAGGGTCATTTGTTTCATCGACATAAGTTGTTTATGTGCAGTCTTTGTTTGGAAGGAAGGAAGGTATGCTTGTCATTCTGTTTGAATATACTTAGTGGTAGGCTGGACATTGAAATTCATAGCAGGTTCTTAGTAGATTGTTACAATAACTAATTATTAGTGTGGACCAGGGTGTATCTGACATTTTCTTTGATTGATTGCAACCATGGTACTGATTAGCTTTATTAGTGTGTCTCAGATTTTTATTTGTGAACAAAAGCTATATAGTAGAGCACAGTTGAATCAGCATATAAACACTGGTGACTCTGAGGTGGACGGAAGTGAGAGTGAGAGAGGTGGTTTCACAGGGCATCCTATGTGTGAATTTTGCAGAACTCCTTTTTACGGAGATAATGAGCTGTATACCCATATGTCAACTGAGCATTATACATGTCATATATGCCAAAGGTAAAGTAAACCTGTTTTGATTTGTGATTGGGGTGTATTAATTTAAACCAAATTGTTTGTCCTAAGCAAACTGAACTACACCAAAATGGTTTCATCTGGTTTTTTATGTGAAGAATTTTCAATCCATTTTCTTCATTGACTTAGAAACTCATTTAGTTATAACTCAATTTCTATTAACTTTCCATATACTTCATTTCTGTTAGTAAATATCACGAAAATAGGTGTTTTAGAATGTGTTAGACCCCCAATCATTAAGACATATAGTAGAAAGGGTAAAGGGGTAATTGTAGAGGGAGATGGCCATAACGGCCAAGAGAGAGAAGAGGGGACGAGTGGAGAGATGTAGGGAATGGGCCGTTGTCTATAAGGCCAGAGAATGTAAGGGGGGAAGGATCATTCTAGACTTTTGTGCTTTTGCAGCTTTTGAGTTTGTTGGGAGAGAATACCCACCCTCTCGGATTGGTGGGTCTGTTTCTGATTGCCTAAGGGCTCTTTTGTTGGTTTTGTAATGCCTACGGGCTCTTTTCTAAGAAGTAAAACATCTTGTGAGGGGTTGATACCTCACAGAATGATAAAAGAAAATGGATTCAAATAACTGAACCAATCAGTTCTGTCTGTTTCAAATTTTCAGATCTGGTGGTTTGGTTTACTCTTCTGCTTCAGCTAATCAAAGCTTCCAATAACTTGGATAAGATAATAGAATTGTACCAAACTGATTAGACCTCTATCATTCTTTATAATTGTTAACTTAGTGAGATTAAAATTTGTTTTCCTTTTCTTATAAATGTTTTATTCTTTTTCTTGGGTTGCAGGCTTCATCCTGGACAGTATGAATACTATAAGAATTATGATGACCTAGAGGCAAGTTGTAATACCCATACCCCCTTTCAACGATGAAGTACTGTCTTTTTAATCACTGTTTTGAGTTGAGAATGTATCTAATGGCAGTGGAATTATTTTTCACTACAGATCCATTTCCGACAAGCCCATTTTCTTTGTGAAGATGAAGCCTGCCTTGCTAAAAAGTTTATAGTCTTCCAATCTGAAGGAGAAATGAAGGTAACTTAGAAGATTTGATCAAGTATTTTGTATGTGTGTATATAGTTAAGTGCTTTCTATTTTGTTTTTGCTCACTTCTTTCAAGTTTTTAAGGTTCTAGGCTATATCCATCACAGTGCGTTGTTTCTTGATGTCAAATTGTTCTTTTTCAGAGGCATAATACTATTGAACATGGTGGCAAGCTATCTCGATCAAAGCGAAACGCTGCTTTGCAGGTATGTACTACTGCTCTCCCTATTATAGTCTTTAAGAAATGTAATTTGTGTTGGGGGGTGGGGGTGGGGGTTGGCCATGTCATTTTTTATGCTTGCATGTTCCTAGAATAGAAAACAGGAAAGAGAGAAGAGAACGTAGTAATATTTCACTTATGTTTCTCGATTCCCTTTCATGCACTATTTCTCAATTACTTTACTTTTTTCTAGATACCAACCAGTTTTCGGTATCGAAGAAGTAATGAGCAAGATAATCGCCGTGGTAGACGGACATTTCGTCGGGATTCCTCTGATGACCTACTTTCTTTAGCCATACAAGCAAGTTTTGAAACAGGCAATGCTGATGACACCAATCATGACCCACTGCCTTCATCATCATTTTCTAGTGGACAAGTAGCCTCGGATCAGGGAAATATAAGCAACATTGATCCCTTAATTGAACCATTTGAATCATTGGCAACAACAGATCCTGAATCAGCTTCTAGATACCTTCAAGCCTTGGGTCACTCTAGGAATTCACAATTGGAGCAATCATCCTTTCCTCCCCTTTCAACAGCACCTAGCAGCAGCCATCCGAAGCCCATGCATGACAAAGATGCTTCGCAAAATAACAGTATGGCAGCTCATCTTCGCCGCCAGAGGAAAGTGACTGTTCTGAATTCTGCTCAAGGTTGGCCAAAATCAGGTCGTGCCCCTGTGCTACCATCAAATAACTCATCTCAAGCTTGGCCTGCAATAAATGTTAATCATGCAGCATCAAGCAGTTCTGGGCAGGCTAAAGGTGTTGTCACTATCAGTAATGGCCCTTCAGTCTCAGGATACTCGAATTCTGCTCAGATGCATGGGAAGGCTCTATCTAGTTCTTCGAGTGGATCAAGCAGCTCTGGGCAAACTAAGGGTGTTGTCACTATCAGTAATGGCCCTTCAGTCTCTGGATACTCAAATGCTGCTCAAGTGCAGGGGAAGGGTCGATCTAGTTCTTCGAGTGGATTAGGTAGTAGCAGTCGGATCAGCCACTCTGCTTCTGCCCCTAGTCTTACCGACATTGCCTATGCCGAACCCTCAGTTAATGATTTCCCTCCAGTTTCTGCAGCACAGGCACGGAAGGCGCCCTCAAGTAGCCAGAGTTCACTGAACGTGGAAGATGTCCAAACAGCAAACAAATCTCTAGTTGAAAAGATACGTGCTGCTCTCGATTTCGACCAAGATAGATATGCCGTTTTTAAAGATATATCTGCACAATATCGTCAGGGTCTGATTGAGACGCAAATGTATCTTGATTGTGTGCAACAATTTGGTTTGTCACATCTTGTCCTTGAGCTTGCTAGGCTCTGTCCTGATGCTCAGAAACAGAAAGAACTTGTGGAGACCTATAATGCTAGTTTACAAAAAGATGTGATCCAAGAAAATGGTAGGGCTCAAGATGGTATTCAAATCAAAGACAAGAGCAAGGGAAAGAAAGGTAAAGGGAAGTATATAGATGCTAAAGACACTAGTTCCAAGGATAAATTAGCAGATAGTATCTTGAGTAGCGTTCGGGAACTGCAGTCAAATTTCATGCCTTCAGAGGAAGAGGTGGAGGTATTATCAAAGGGTGAATACCGTGCTGCGAAAGGAAAATCAAAGTTATCATCTGATGAGCAGCAAGGTGGGACGGGAGGCCAGAACAGTCAACCGTCCACTGGTCTATCAAACCAAAACAACACCGGGGATGGGGGTGGGGGGAGTAAGCAGAAAAAGAAAACCTCCAAGTTCCATAGAGTTCGCTTAGGCGATGGTTCGGTTGCTGCTCTTTTAGACTTAAAAAACCCCAATCCCGCTTCAGACCCAGATCCAGTAGAAAGAATAGATGACAACAATTCTGGGGGTGGGGTGCCTGTCCGTGGCGTCTGGCGAAACGGTGCTCAAAAGCTCTTCTCATAG

mRNA sequence

ATGGACGATAGCTGTGCTGTGTGTGCCGATACTTTGGAATGGGTTGCCTATGGTTCTTGTGGGCATCGGGATGTCTGCTCCACCTGTGTTGCTCGACTTCGATTCATTTGCGGTGATCGCCGCTGTTGCATCTGCAAGTCGGAGTCCGCCGTCGTGTTCGTCACTAAGGCCTTAGGAGATTATACAAGGACGATTAATGACTTTACAGTCTTTCCTTCTGAGCCAAGGGAAGGCCGAGCTGGATCCTATTGGTATCATGAAGACACACAAGCATTTTTCGATGATGTTGACCACTACAAGATGATAAAAGCAATGTGCAGGCTGTCATGCAGCGTCTGTGATAAGATAGGTGAAGATCAACCAAATGATGGACCAAAGCGGCGAGGAAAATTTCGGAACATCGAGCAGTTAAAGGGTCATTTGTTTCATCGACATAAGTTGTTTATGTGCAGTCTTTGTTTGGAAGGAAGGAAGATTTTTATTTGTGAACAAAAGCTATATAGTAGAGCACAGTTGAATCAGCATATAAACACTGGTGACTCTGAGGTGGACGGAAGTGAGAGTGAGAGAGGTGGTTTCACAGGGCATCCTATGTGTGAATTTTGCAGAACTCCTTTTTACGGAGATAATGAGCTGTATACCCATATGTCAACTGAGCATTATACATGTCATATATGCCAAAGGCTTCATCCTGGACAGTATGAATACTATAAGAATTATGATGACCTAGAGATCCATTTCCGACAAGCCCATTTTCTTTGTGAAGATGAAGCCTGCCTTGCTAAAAAGTTTATAGTCTTCCAATCTGAAGGAGAAATGAAGAGGCATAATACTATTGAACATGGTGGCAAGCTATCTCGATCAAAGCGAAACGCTGCTTTGCAGATACCAACCAGTTTTCGGTATCGAAGAAGTAATGAGCAAGATAATCGCCGTGGTAGACGGACATTTCGTCGGGATTCCTCTGATGACCTACTTTCTTTAGCCATACAAGCAAGTTTTGAAACAGGCAATGCTGATGACACCAATCATGACCCACTGCCTTCATCATCATTTTCTAGTGGACAAGTAGCCTCGGATCAGGGAAATATAAGCAACATTGATCCCTTAATTGAACCATTTGAATCATTGGCAACAACAGATCCTGAATCAGCTTCTAGATACCTTCAAGCCTTGGGTCACTCTAGGAATTCACAATTGGAGCAATCATCCTTTCCTCCCCTTTCAACAGCACCTAGCAGCAGCCATCCGAAGCCCATGCATGACAAAGATGCTTCGCAAAATAACAGTATGGCAGCTCATCTTCGCCGCCAGAGGAAAGTGACTGTTCTGAATTCTGCTCAAGGTTGGCCAAAATCAGGTCGTGCCCCTGTGCTACCATCAAATAACTCATCTCAAGCTTGGCCTGCAATAAATGTTAATCATGCAGCATCAAGCAGTTCTGGGCAGGCTAAAGGTGTTGTCACTATCAGTAATGGCCCTTCAGTCTCAGGATACTCGAATTCTGCTCAGATGCATGGGAAGGCTCTATCTAGTTCTTCGAGTGGATCAAGCAGCTCTGGGCAAACTAAGGGTGTTGTCACTATCAGTAATGGCCCTTCAGTCTCTGGATACTCAAATGCTGCTCAAGTGCAGGGGAAGGGTCGATCTAGTTCTTCGAGTGGATTAGGTAGTAGCAGTCGGATCAGCCACTCTGCTTCTGCCCCTAGTCTTACCGACATTGCCTATGCCGAACCCTCAGTTAATGATTTCCCTCCAGTTTCTGCAGCACAGGCACGGAAGGCGCCCTCAAGTAGCCAGAGTTCACTGAACGTGGAAGATGTCCAAACAGCAAACAAATCTCTAGTTGAAAAGATACGTGCTGCTCTCGATTTCGACCAAGATAGATATGCCGTTTTTAAAGATATATCTGCACAATATCGTCAGGGTCTGATTGAGACGCAAATGTATCTTGATTGTGTGCAACAATTTGGTTTGTCACATCTTGTCCTTGAGCTTGCTAGGCTCTGTCCTGATGCTCAGAAACAGAAAGAACTTGTGGAGACCTATAATGCTAGTTTACAAAAAGATGTGATCCAAGAAAATGGTAGGGCTCAAGATGGTATTCAAATCAAAGACAAGAGCAAGGGAAAGAAAGGTAAAGGGAAGTATATAGATGCTAAAGACACTAGTTCCAAGGATAAATTAGCAGATAGTATCTTGAGTAGCGTTCGGGAACTGCAGTCAAATTTCATGCCTTCAGAGGAAGAGGTGGAGGTATTATCAAAGGGTGAATACCGTGCTGCGAAAGGAAAATCAAAGTTATCATCTGATGAGCAGCAAGGTGGGACGGGAGGCCAGAACAGTCAACCGTCCACTGGTCTATCAAACCAAAACAACACCGGGGATGGGGGTGGGGGGAGTAAGCAGAAAAAGAAAACCTCCAAGTTCCATAGAGTTCGCTTAGGCGATGGTTCGGTTGCTGCTCTTTTAGACTTAAAAAACCCCAATCCCGCTTCAGACCCAGATCCAGTAGAAAGAATAGATGACAACAATTCTGGGGGTGGGGTGCCTGTCCGTGGCGTCTGGCGAAACGGTGCTCAAAAGCTCTTCTCATAG

Coding sequence (CDS)

ATGGACGATAGCTGTGCTGTGTGTGCCGATACTTTGGAATGGGTTGCCTATGGTTCTTGTGGGCATCGGGATGTCTGCTCCACCTGTGTTGCTCGACTTCGATTCATTTGCGGTGATCGCCGCTGTTGCATCTGCAAGTCGGAGTCCGCCGTCGTGTTCGTCACTAAGGCCTTAGGAGATTATACAAGGACGATTAATGACTTTACAGTCTTTCCTTCTGAGCCAAGGGAAGGCCGAGCTGGATCCTATTGGTATCATGAAGACACACAAGCATTTTTCGATGATGTTGACCACTACAAGATGATAAAAGCAATGTGCAGGCTGTCATGCAGCGTCTGTGATAAGATAGGTGAAGATCAACCAAATGATGGACCAAAGCGGCGAGGAAAATTTCGGAACATCGAGCAGTTAAAGGGTCATTTGTTTCATCGACATAAGTTGTTTATGTGCAGTCTTTGTTTGGAAGGAAGGAAGATTTTTATTTGTGAACAAAAGCTATATAGTAGAGCACAGTTGAATCAGCATATAAACACTGGTGACTCTGAGGTGGACGGAAGTGAGAGTGAGAGAGGTGGTTTCACAGGGCATCCTATGTGTGAATTTTGCAGAACTCCTTTTTACGGAGATAATGAGCTGTATACCCATATGTCAACTGAGCATTATACATGTCATATATGCCAAAGGCTTCATCCTGGACAGTATGAATACTATAAGAATTATGATGACCTAGAGATCCATTTCCGACAAGCCCATTTTCTTTGTGAAGATGAAGCCTGCCTTGCTAAAAAGTTTATAGTCTTCCAATCTGAAGGAGAAATGAAGAGGCATAATACTATTGAACATGGTGGCAAGCTATCTCGATCAAAGCGAAACGCTGCTTTGCAGATACCAACCAGTTTTCGGTATCGAAGAAGTAATGAGCAAGATAATCGCCGTGGTAGACGGACATTTCGTCGGGATTCCTCTGATGACCTACTTTCTTTAGCCATACAAGCAAGTTTTGAAACAGGCAATGCTGATGACACCAATCATGACCCACTGCCTTCATCATCATTTTCTAGTGGACAAGTAGCCTCGGATCAGGGAAATATAAGCAACATTGATCCCTTAATTGAACCATTTGAATCATTGGCAACAACAGATCCTGAATCAGCTTCTAGATACCTTCAAGCCTTGGGTCACTCTAGGAATTCACAATTGGAGCAATCATCCTTTCCTCCCCTTTCAACAGCACCTAGCAGCAGCCATCCGAAGCCCATGCATGACAAAGATGCTTCGCAAAATAACAGTATGGCAGCTCATCTTCGCCGCCAGAGGAAAGTGACTGTTCTGAATTCTGCTCAAGGTTGGCCAAAATCAGGTCGTGCCCCTGTGCTACCATCAAATAACTCATCTCAAGCTTGGCCTGCAATAAATGTTAATCATGCAGCATCAAGCAGTTCTGGGCAGGCTAAAGGTGTTGTCACTATCAGTAATGGCCCTTCAGTCTCAGGATACTCGAATTCTGCTCAGATGCATGGGAAGGCTCTATCTAGTTCTTCGAGTGGATCAAGCAGCTCTGGGCAAACTAAGGGTGTTGTCACTATCAGTAATGGCCCTTCAGTCTCTGGATACTCAAATGCTGCTCAAGTGCAGGGGAAGGGTCGATCTAGTTCTTCGAGTGGATTAGGTAGTAGCAGTCGGATCAGCCACTCTGCTTCTGCCCCTAGTCTTACCGACATTGCCTATGCCGAACCCTCAGTTAATGATTTCCCTCCAGTTTCTGCAGCACAGGCACGGAAGGCGCCCTCAAGTAGCCAGAGTTCACTGAACGTGGAAGATGTCCAAACAGCAAACAAATCTCTAGTTGAAAAGATACGTGCTGCTCTCGATTTCGACCAAGATAGATATGCCGTTTTTAAAGATATATCTGCACAATATCGTCAGGGTCTGATTGAGACGCAAATGTATCTTGATTGTGTGCAACAATTTGGTTTGTCACATCTTGTCCTTGAGCTTGCTAGGCTCTGTCCTGATGCTCAGAAACAGAAAGAACTTGTGGAGACCTATAATGCTAGTTTACAAAAAGATGTGATCCAAGAAAATGGTAGGGCTCAAGATGGTATTCAAATCAAAGACAAGAGCAAGGGAAAGAAAGGTAAAGGGAAGTATATAGATGCTAAAGACACTAGTTCCAAGGATAAATTAGCAGATAGTATCTTGAGTAGCGTTCGGGAACTGCAGTCAAATTTCATGCCTTCAGAGGAAGAGGTGGAGGTATTATCAAAGGGTGAATACCGTGCTGCGAAAGGAAAATCAAAGTTATCATCTGATGAGCAGCAAGGTGGGACGGGAGGCCAGAACAGTCAACCGTCCACTGGTCTATCAAACCAAAACAACACCGGGGATGGGGGTGGGGGGAGTAAGCAGAAAAAGAAAACCTCCAAGTTCCATAGAGTTCGCTTAGGCGATGGTTCGGTTGCTGCTCTTTTAGACTTAAAAAACCCCAATCCCGCTTCAGACCCAGATCCAGTAGAAAGAATAGATGACAACAATTCTGGGGGTGGGGTGCCTGTCCGTGGCGTCTGGCGAAACGGTGCTCAAAAGCTCTTCTCATAG

Protein sequence

MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGDSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNYDDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSFRYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASDQGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPMHDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSSSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSNAAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQSSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLSHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDAKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQNSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVERIDDNNSGGGVPVRGVWRNGAQKLFS
Homology
BLAST of Moc09g05900 vs. NCBI nr
Match: XP_022148262.1 (E3 ubiquitin-protein ligase HEL2 [Momordica charantia])

HSP 1 Score: 1684.5 bits (4361), Expect = 0.0e+00
Identity = 865/865 (100.00%), Postives = 865/865 (100.00%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD
Sbjct: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM
Sbjct: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420

Query: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480
           HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS
Sbjct: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480

Query: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540
           SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN
Sbjct: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540

Query: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600
           AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ
Sbjct: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600

Query: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660
           SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS
Sbjct: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720
           HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA
Sbjct: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720

Query: 721 KDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQ 780
           KDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQ
Sbjct: 721 KDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQ 780

Query: 781 NSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVER 840
           NSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVER
Sbjct: 781 NSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVER 840

Query: 841 IDDNNSGGGVPVRGVWRNGAQKLFS 866
           IDDNNSGGGVPVRGVWRNGAQKLFS
Sbjct: 841 IDDNNSGGGVPVRGVWRNGAQKLFS 865

BLAST of Moc09g05900 vs. NCBI nr
Match: XP_038897973.1 (E3 ubiquitin-protein ligase HEL2 [Benincasa hispida])

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 737/868 (84.91%), Postives = 768/868 (88.48%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGR+GSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRSGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PND  KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRK+FICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHP+CEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPICEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQ HFLCEDEACLAKKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEACLAKKFIVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQ SFET N DDTNHDPLP     SGQ ASD
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDTNHDPLP-----SGQGASD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           Q NISNIDPLIEPFE+LATTDPESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP 
Sbjct: 361 QENISNIDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPS 420

Query: 421 HDKDASQNNSMAAHLRRQR-KVTVLNSAQGWPKSGRAPVL-PSNNSSQAWPAINVNHAAS 480
            DKDA  N+SMAAHLRRQR  VTVLNSA GWPKS RAPVL PSNNSSQAWPAIN NHAAS
Sbjct: 421 QDKDAIHNDSMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPPSNNSSQAWPAINANHAAS 480

Query: 481 SSSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGY 540
           SSS Q KGVVTI+NGPSVS YSN+AQMH KA                             
Sbjct: 481 SSSRQTKGVVTINNGPSVSAYSNAAQMHAKA----------------------------- 540

Query: 541 SNAAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSS 600
                     RSSSSSG GSSSRISHSASAPSLTDIAY EPSVN+FPPVS  QA KAPSS
Sbjct: 541 ----------RSSSSSGSGSSSRISHSASAPSLTDIAYTEPSVNEFPPVSTVQAWKAPSS 600

Query: 601 SQSSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFG 660
           SQSS+N+EDVQTANKSLVEKIRAALDFDQDRYA+FKDISAQYRQG I+T+MYLDCVQQFG
Sbjct: 601 SQSSMNMEDVQTANKSLVEKIRAALDFDQDRYAIFKDISAQYRQGQIDTEMYLDCVQQFG 660

Query: 661 LSHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYI 720
           LSHL+LELARLCPD QKQKELVETYNAS  KDVI  NGRAQD IQIKD SKGKKGKGK I
Sbjct: 661 LSHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSIQIKDNSKGKKGKGKSI 720

Query: 721 DAKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTG 780
           + KD+SSKDKLADSILSSVRELQS+F P EE+VEVLSKGEYRA+KGK K+SSD QQGGTG
Sbjct: 721 EVKDSSSKDKLADSILSSVRELQSSFRPLEEDVEVLSKGEYRASKGKLKISSDNQQGGTG 780

Query: 781 GQNSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPV 840
           GQNSQ STGL N++    GGGGSKQKKKTSKFHRVRLGDGSVAALLDLKN N  SDPDPV
Sbjct: 781 GQNSQLSTGLLNESTGDGGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNLTSDPDPV 823

Query: 841 ERIDD-NNSGGGVPVRGVWRNGAQKLFS 866
           ER++  NN+ G +PVRGVWRNGAQ+LFS
Sbjct: 841 ERVEGRNNATGVLPVRGVWRNGAQRLFS 823

BLAST of Moc09g05900 vs. NCBI nr
Match: XP_023547810.1 (E3 ubiquitin-protein ligase HEL2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 728/871 (83.58%), Postives = 765/871 (87.83%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCAD LEWVAYGSCGHRDVCSTCVARLRFICGDR CCICKSES VVFVTKALGD
Sbjct: 1   MDDSCAVCADALEWVAYGSCGHRDVCSTCVARLRFICGDRCCCICKSESTVVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGR GSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRCGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PNDG KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCE+CRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEYCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQAHFLCEDE CL+KKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDETCLSKKFIVFQSESEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLS+AIQ SFET N DDT HDPLP     SGQV SD
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSVAIQESFETANVDDTTHDPLP-----SGQVDSD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           QGNISNIDPLIEPFESL+TTD ESASRYLQALGHSRNSQL QSSFPPLSTA SSSHPKP+
Sbjct: 361 QGNISNIDPLIEPFESLSTTDSESASRYLQALGHSRNSQLGQSSFPPLSTASSSSHPKPI 420

Query: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480
            DKD   NNSMAAHLRR+R VTV+N AQGWPKS RAPV+PSN SSQAWPAINVNH ASSS
Sbjct: 421 QDKD---NNSMAAHLRRKRNVTVINPAQGWPKSSRAPVVPSNKSSQAWPAINVNHTASSS 480

Query: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540
           SGQ KGVVT+SNG S SGYSN+AQMH K  +SSSSGS S                     
Sbjct: 481 SGQTKGVVTVSNGSSGSGYSNAAQMHAKGKTSSSSGSGS--------------------- 540

Query: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600
                            SSSRISHSASAP+LTD+AY EPSVNDFPPVSAAQ+RK PSSSQ
Sbjct: 541 -----------------SSSRISHSASAPNLTDMAYTEPSVNDFPPVSAAQSRKVPSSSQ 600

Query: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660
           SS+N EDVQTANKSLVEKIRAALDFDQDRYA+FKDISAQYRQG I+ +MYLDCVQQFGLS
Sbjct: 601 SSMNTEDVQTANKSLVEKIRAALDFDQDRYAIFKDISAQYRQGQIDPEMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720
           HLVLELARLCPDAQKQKELV  YNASLQK+VIQENGRAQD IQIKDK KG+KGKGK  D 
Sbjct: 661 HLVLELARLCPDAQKQKELVGIYNASLQKEVIQENGRAQDSIQIKDKGKGRKGKGKSTDV 720

Query: 721 KDT----SSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGG 780
           KD+    SSKD+LADSILSSVRELQS+  P EE+VEVLSKGEYR  KGK+K+SSD+QQ G
Sbjct: 721 KDSSSKDSSKDRLADSILSSVRELQSSLRPLEEDVEVLSKGEYRGLKGKTKISSDDQQSG 780

Query: 781 TGGQNSQPSTGLSNQNNTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDP 840
           TGGQN QPSTGLSN  +TGDG GGGSKQ+KKTSKFHRVRLGDGSVAALLDLKN NPASD 
Sbjct: 781 TGGQNRQPSTGLSNHQSTGDGDGGGSKQRKKTSKFHRVRLGDGSVAALLDLKNSNPASDS 825

Query: 841 DPVERIDD-NNSGGGVPVRGVWRNGAQKLFS 866
           DP ER++D NN+ G VPVRGVWRNGAQ+LFS
Sbjct: 841 DPSERVEDGNNTAGAVPVRGVWRNGAQRLFS 825

BLAST of Moc09g05900 vs. NCBI nr
Match: XP_022954166.1 (E3 ubiquitin-protein ligase HEL2 [Cucurbita moschata])

HSP 1 Score: 1399.8 bits (3622), Expect = 0.0e+00
Identity = 730/871 (83.81%), Postives = 763/871 (87.60%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCAD LEWVAYGSCGHRDVCSTCVARLRFICGDR CCICKSES VVFVTKALGD
Sbjct: 1   MDDSCAVCADALEWVAYGSCGHRDVCSTCVARLRFICGDRCCCICKSESTVVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGR GSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRCGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PNDG KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCE+CRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEYCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQAHFLCEDE CL+KKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDETCLSKKFIVFQSESEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLS+AIQ SFET N DDT HDPLP     SGQV SD
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSVAIQESFETANVDDTTHDPLP-----SGQVDSD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           QGNISNIDPLIEPFESL+TTD ESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP+
Sbjct: 361 QGNISNIDPLIEPFESLSTTDAESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPI 420

Query: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480
            DKD   NNSMAAHLRR+R VTV+N AQGWPKS RAPV+PSN SSQAWPAINVNH ASSS
Sbjct: 421 QDKD---NNSMAAHLRRKRNVTVINPAQGWPKSSRAPVVPSNKSSQAWPAINVNHTASSS 480

Query: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540
           SGQ KG VTISNG S SGYSN+AQMH K  +SSSSGS S                     
Sbjct: 481 SGQTKGAVTISNGSSGSGYSNAAQMHVKGKTSSSSGSGS--------------------- 540

Query: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600
                            SSSRISHSASAP+LTD+AY EPSVNDFPPVSAAQARK PSSSQ
Sbjct: 541 -----------------SSSRISHSASAPNLTDMAYTEPSVNDFPPVSAAQARKVPSSSQ 600

Query: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660
           SS+N EDVQTANKSLVEKIRAALDFDQDRYA+FKDISAQYRQG I+ +MYLDCVQQFGLS
Sbjct: 601 SSMNTEDVQTANKSLVEKIRAALDFDQDRYAIFKDISAQYRQGQIDPEMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720
           HLVLELARLCPDAQKQKELV  YNASLQK+VIQENGRAQD IQIKDK KGKKGKGK  D 
Sbjct: 661 HLVLELARLCPDAQKQKELVGIYNASLQKEVIQENGRAQDSIQIKDKDKGKKGKGKSTDV 720

Query: 721 KD----TSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGG 780
           KD     SSKD+LAD ILSSVRELQS+  P EE+VEVLSKGEYRA KGK+K+SSD+QQ G
Sbjct: 721 KDRSSKDSSKDRLADGILSSVRELQSSLRPLEEDVEVLSKGEYRALKGKTKISSDDQQSG 780

Query: 781 TGGQNSQPSTGLSNQNNTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDP 840
           TGGQN Q STGLSN  +TGDG GGGSKQ+KKTSKFHRVRLGDGSVAALLDLKN NPASD 
Sbjct: 781 TGGQNRQTSTGLSNHQSTGDGDGGGSKQRKKTSKFHRVRLGDGSVAALLDLKNSNPASDS 825

Query: 841 DPVERIDD-NNSGGGVPVRGVWRNGAQKLFS 866
           DP ER++D NN+ G VPVRGVWRNGAQ+LFS
Sbjct: 841 DPSERVEDGNNTAGAVPVRGVWRNGAQRLFS 825

BLAST of Moc09g05900 vs. NCBI nr
Match: XP_004135963.1 (E3 ubiquitin-protein ligase ZNF598 [Cucumis sativus] >KGN45064.1 hypothetical protein Csa_015726 [Cucumis sativus])

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 724/869 (83.31%), Postives = 766/869 (88.15%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEP+EGR GSYWYHEDTQAFFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRCGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PND  KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRK+FICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQ HFLCEDEACLAKKF+VFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEACLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLSLA+Q SFET N DD NHDPLP     SGQVASD
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSLALQESFETANVDDNNHDPLP-----SGQVASD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           Q N+SN+DPLIE FE+LATTDPESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP 
Sbjct: 361 QENLSNVDPLIESFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPN 420

Query: 421 HDKDASQNNSMAAHLRRQR-KVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASS 480
            DKD   NNSMAAHLRRQR  VTVLNSA GWPKS RAPVLPSNNSSQAWPAIN NHAASS
Sbjct: 421 QDKDIIHNNSMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHAASS 480

Query: 481 SSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYS 540
           SSGQ KGV TI+NGPSVS Y+N+AQMH K                               
Sbjct: 481 SSGQTKGVATINNGPSVSAYANAAQMHPKP------------------------------ 540

Query: 541 NAAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSS 600
                    RS+SSSG GSSSRISHSASAP+LTDIA+ EPSVN+FPPVSAA ARK PSSS
Sbjct: 541 ---------RSTSSSGSGSSSRISHSASAPNLTDIAHTEPSVNEFPPVSAAHARKVPSSS 600

Query: 601 QSSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGL 660
           QSS+N+EDVQTANKSLVEKIRAALDFDQDRY++FKDISAQYRQG I+T+MYLDCVQQFGL
Sbjct: 601 QSSMNMEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQGQIDTEMYLDCVQQFGL 660

Query: 661 SHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYID 720
           SHL+LELARLCPD QKQKELVETYNAS  KDV   NGRAQD IQIKDKSKGKKGKGK I+
Sbjct: 661 SHLLLELARLCPDPQKQKELVETYNASFHKDVFPVNGRAQDSIQIKDKSKGKKGKGKSIE 720

Query: 721 AKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGG 780
            KD+SSKDKLADSI+SSVRELQS++ P +E+VEVLSKGEYR +KGK K+SSD+QQGGTG 
Sbjct: 721 VKDSSSKDKLADSIMSSVRELQSSYRPPDEDVEVLSKGEYRTSKGKLKISSDDQQGGTGR 780

Query: 781 QNSQPSTGLSNQN--NTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDP 840
           Q SQPSTGLSNQ+  + G GGGGSKQKKKTSKFHRVRLGDGSVAALLDLKN N  SDPDP
Sbjct: 781 QKSQPSTGLSNQSTGDGGGGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNLGSDPDP 824

Query: 841 VERIDDNNSG-GGVPVRGVWRNGAQKLFS 866
            ER++D N+G G +PVRGVWRNGAQKLFS
Sbjct: 841 DERVEDRNNGAGALPVRGVWRNGAQKLFS 824

BLAST of Moc09g05900 vs. ExPASy Swiss-Prot
Match: Q76PD2 (E3 ubiquitin-protein ligase hel2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC1223.01 PE=1 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 2.1e-38
Identity = 97/313 (30.99%), Postives = 151/313 (48.24%), Query Frame = 0

Query: 5   CAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTRT 64
           C +CA+ + +     C HR +C  C  RLR +   + C  CK+E   V +TK   D+   
Sbjct: 81  CFICAEGITYSCVLPCNHR-MCHVCALRLRALYKTKECTFCKTEWDTVLITK---DHEID 140

Query: 65  INDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPNDG 124
           I+D  +     ++ + G  +  E  Q   + +  +   +  C ++C              
Sbjct: 141 IHDVDLAKLPFQDEKLGIVYSDEHAQEESNLLLQFNCPEDACDITC-------------- 200

Query: 125 PKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGDSEVD 184
              +G F     LK H   +H  F C LC++ +K+F  E  L+S+  L +H   GD    
Sbjct: 201 ---KGWF----DLKLHAKVKHHKFFCDLCVKNKKVFTHEHTLFSKKGLTKHNEVGD---Q 260

Query: 185 GSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRL-HPGQYEYYKNYDDL 244
           GS+ E  GF GHP CEFC T FY D+EL+ H   +H  C+IC ++     ++Y+KNYD L
Sbjct: 261 GSDLEITGFKGHPKCEFCNTHFYDDDELFKHCREKHERCYICDQVAGRPTHQYFKNYDSL 320

Query: 245 EIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSFRYR 304
           E HF + H++C +  CL +KF+VF +E ++K H   EH    ++ +   A +I   F Y 
Sbjct: 321 ERHFEKDHYICRERECLERKFVVFGTEIDLKAHQLDEHPHNFTQRELREARRIIPQFSYD 365

Query: 305 RSNEQDNRRGRRT 317
                   R  RT
Sbjct: 381 PPGASGRNRRERT 365

BLAST of Moc09g05900 vs. ExPASy Swiss-Prot
Match: Q86UK7 (E3 ubiquitin-protein ligase ZNF598 OS=Homo sapiens OX=9606 GN=ZNF598 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 2.6e-33
Identity = 101/361 (27.98%), Postives = 159/361 (44.04%), Query Frame = 0

Query: 4   SCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTR 63
           SC +C   LE  A G C H  VC  C  ++R +C  R C +C+ E   V   K L     
Sbjct: 28  SCVLCCGDLEATALGRCDH-PVCYRCSTKMRVLCEQRYCAVCREELRQVVFGKKL----- 87

Query: 64  TINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPND 123
               F   P    +       + +    +F D   Y + + + +  C  C ++       
Sbjct: 88  --PAFATIPIHQLQ-------HEKKYDIYFADGKVYALYRQLLQHECPRCPEL------- 147

Query: 124 GPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGDSEV 183
                  F     L+ H+  +H+LF C LCL+  +IF  E+K YSR  L +H   GD + 
Sbjct: 148 -----PPFSLFGDLEQHMRRQHELFCCRLCLQHLQIFTYERKWYSRKDLARHRMQGDPD- 207

Query: 184 DGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNYDDL 243
               S R    GHP+C+FC   +  ++EL  H+  +HY CH C     G  +YY +Y  L
Sbjct: 208 --DTSHR----GHPLCKFCDERYLDNDELLKHLRRDHYFCHFCD--SDGAQDYYSDYAYL 267

Query: 244 EIHFRQAHFLCEDEACLAKKFI-VFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF-- 303
             HFR+ HFLCE+  C  ++F   F++E ++K H T  H    + +++N  + +  S+  
Sbjct: 268 REHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHRTACHSRSRAEARQNRHIDLQFSYAP 327

Query: 304 RYRRSNE----------------------------QDNRRGRRTFRRDSSDDLLSLAIQA 334
           R+ R NE                            Q +RRG   ++R+  D  ++ A++A
Sbjct: 328 RHSRRNEGVVGGEDYEEVDRYSRQGRVARAGTRGAQQSRRGSWRYKREEEDREVAAAVRA 352

BLAST of Moc09g05900 vs. ExPASy Swiss-Prot
Match: Q6PFK1 (E3 ubiquitin-protein ligase ZNF598 OS=Danio rerio OX=7955 GN=znf598 PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 9.3e-31
Identity = 129/517 (24.95%), Postives = 217/517 (41.97%), Query Frame = 0

Query: 2   DDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSE-SAVVFVTKALGD 61
           + +C +C   ++  A G C H  VC  C  ++R +C  + C +C+ +   VVF+ K    
Sbjct: 54  ESTCVLCCQDIDLFAVGKCDH-PVCYRCSTKMRVLCEQKYCAVCREQLDKVVFLRKPEAF 113

Query: 62  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 121
            T  I+                Y   +    +F D   +   + +    C  C +     
Sbjct: 114 ATLNIH---------------HYQCEKKYDIYFGDGKVHAQFRKILLNECPHCPE----- 173

Query: 122 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 181
               PK   KF  +EQ   H+  +H+LF C LCL+  KIF  E+K YSR  L +H   GD
Sbjct: 174 ----PKVFSKFEELEQ---HMRKQHELFCCKLCLKHLKIFSYERKWYSRKDLARHRMQGD 233

Query: 182 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 241
            +     S R    GHP+C+FC   +  ++EL  H+  +HY CH C     G  EYY +Y
Sbjct: 234 PD---DTSHR----GHPLCKFCDDRYLDNDELLKHLRRDHYFCHFCDA--DGAQEYYSDY 293

Query: 242 DDLEIHFRQAHFLCEDEACLAKKFI-VFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTS 301
             L  HFR++H+LCE+  C  ++F   F++E + K H    H    + +++N  + I  +
Sbjct: 294 QYLSEHFRESHYLCEEGRCSTEQFTHAFRTEIDYKAHKAAAHSKNRAEARQNRQIDIQFN 353

Query: 302 FRYRRSNEQD------------NRRGR-------------RTFR--RDSSDDLLSLAIQA 361
           +  R+    D            NR+GR             R++R  R+  D  ++ A++A
Sbjct: 354 YAPRQQRRNDVGGDDYEEVDRFNRQGRPGRGRGPGGQQNLRSWRYNREEEDREMAAAMRA 413

Query: 362 SF---------------------ETGNADDTNHDPLPSSSFSSGQVASDQGNISNIDPLI 421
           S                      E    D+T ++   +   +  Q  S + N S      
Sbjct: 414 SMASHQEERSHAQERSMLKPRREEKLEPDETRNNRSTARPTNDTQARSMKSNGSLAG--- 473

Query: 422 EPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPMHDKDASQNNS- 463
           + F  L    P +    +Q++    +  L++  FP LS +  SS   P +     +++S 
Sbjct: 474 QDFPVLGAGAPPAP---VQSMIQKPSVSLKEDDFPSLSGSVVSSPMTPAYTNQPRKHSSF 527

BLAST of Moc09g05900 vs. ExPASy Swiss-Prot
Match: Q05580 (E3 ubiquitin-protein ligase HEL2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HEL2 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.3e-29
Identity = 147/580 (25.34%), Postives = 248/580 (42.76%), Query Frame = 0

Query: 2   DDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSES-AVVFVTKALGD 61
           ++ C +CA  L +V+   C H+  C  C  R R +   + C IC++E+  V+F  +  GD
Sbjct: 61  NELCVICARKLTYVSLTPCHHK-TCHICGFRQRALYNKKSCLICRTENEEVMFTDRIDGD 120

Query: 62  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSC--SVCDKIGE 121
            +   N         +  + G  +  E+        +   ++K  C LS    VCD    
Sbjct: 121 ISDKYNFC------EKNEKYGINFTSEEVA-----TETLNLLKFFCPLSKDEQVCD---- 180

Query: 122 DQPNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINT 181
                       F + ++   HL   H   +C +C   +  F CE +++++ QL  H   
Sbjct: 181 ------------FGSFKKYNEHLKSEHNRMICLICATHKHAFPCELEIFTQNQLRNHQTK 240

Query: 182 GDSEVDGSESERGGFTGHPMCEFCR-TPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYY 241
           G+SE         GF GHPMC FC    FY D+ELY HM  +H  CHIC +++P   +Y+
Sbjct: 241 GNSE---------GFKGHPMCAFCSGKRFYSDDELYIHMRNQHEKCHICDKMNPASPQYF 300

Query: 242 KNYDDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGG------KLSRSKRN 301
           K+Y+ L  HF+ +H++C  + CL  KF+VF+ E E++ H   EHG       K  +S+ +
Sbjct: 301 KDYNQLFDHFKHSHYVCTVQTCLDNKFVVFKDELELQAHILQEHGNILKGKPKFFQSELS 360

Query: 302 AALQIPTSFRYRR--------SNEQDNRRGRRTFRRDSS---DDLLSLAIQASFETGNA- 361
             +  P+     R        S+   +  G RT  R +S   +  L LA +A +   N+ 
Sbjct: 361 TFISAPSRVIRERDDYDLPSISSLPGSSSGSRTDVRSASSPEESRLRLAERAKYYLENSK 420

Query: 362 DDTNHDPLPSSSFSSGQVASDQGNISNIDPLIEP-------FESLATTDPESASRY--LQ 421
           +D N     +  +S G++++++   S      +P         +LA T P+++S+Y  L 
Sbjct: 421 EDFNKFSSYNEDYSKGRLSAEKLLESYKLLFTKPNADVYLLIHNLAETFPKNSSKYNNLN 480

Query: 422 ALGHSRNSQL-EQSSFPPLSTAPSSSHPKPMHDKDASQNNSMAAHLRRQRKVTVLNSAQG 481
           A+   R   L  Q+S P LS+  S S          + +   A      R +  L + + 
Sbjct: 481 AIYEQREQTLARQTSLPSLSSDSSLSMSIGRGHWGGTNDGGSAGAALGVRNIKNLPTLKS 540

Query: 482 WPKSGRAPVLPSNNSSQAWPAINVNHAASSSSGQAKGVVTISNGPSVSGYSNSAQMHGKA 541
            P +   P   +   +   P  N+      S        T++  P+         +  K 
Sbjct: 541 -PSASYDPFATTVKKNTLRPVQNIKRTTPQSVSYRTSTNTVAFSPT--------YLESKK 590

Query: 542 LSSSSSGSSSSGQTKGVVTISNGPSVSGYSNAAQVQGKGR 550
            SSS+S ++S  + K +    N P +       Q+ G  R
Sbjct: 601 GSSSTSLNNSKDKLKSL----NLPQLPPPKPKVQIPGLNR 590

BLAST of Moc09g05900 vs. ExPASy Swiss-Prot
Match: Q80YR4 (E3 ubiquitin-protein ligase ZNF598 OS=Mus musculus OX=10090 GN=Znf598 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.3e-24
Identity = 199/856 (23.25%), Postives = 297/856 (34.70%), Query Frame = 0

Query: 4   SCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGDYTR 63
           SC +C   LE  A G C H  VC  C  ++R +C  R C +C+ E   V   K L     
Sbjct: 26  SCVLCCGDLEATALGRCDH-PVCYRCSTKMRVLCEQRYCAVCREELRQVVFGKKL----- 85

Query: 64  TINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQPND 123
               F + P    +       + +    +F D   + + + + +  C  C       P+ 
Sbjct: 86  --PAFALIPIHQLQ-------HEKKYDIYFADGKVFALYRQLLQHECPRC-------PHL 145

Query: 124 GPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGDSEV 183
            P     F     L+ H+  +H+LF C LCL+  KIF  E+K YSR  L +H   GD + 
Sbjct: 146 PP-----FSLFGDLEQHMRKQHELFCCKLCLKHLKIFTYERKWYSRKDLARHRMQGDPD- 205

Query: 184 DGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNYDDL 243
               S R    GHP+C+FC   +  ++EL  H+  +HY CH C     G  +YY +Y  L
Sbjct: 206 --DTSHR----GHPLCKFCDERYLDNDELLKHLRRDHYFCHFCD--SDGAQDYYSDYAYL 265

Query: 244 EIHFRQAHFLCEDEACLAKKFI-VFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF-- 303
             HFR+ HFLCE+  C  ++F   F++E ++K H T  H    + +++N  + +  SF  
Sbjct: 266 REHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHKTACHSRSRAEARQNRQIDLQFSFAP 325

Query: 304 -------------------RYRRSNE---------QDNRRGRRTFRRDSSDDLLSLAIQA 363
                              RY R            Q NRRG   ++R+  D  ++ AI+A
Sbjct: 326 RHSRRSEGVVSGEDYEEVDRYNRQGRAGRASGRGAQQNRRGSWRYKREEEDREVAAAIRA 385

Query: 364 SF------ETGNADDTNHDPLPSSSFSSGQV------------ASDQGNISNIDPLIEPF 423
           S       ET   +D      P    ++ +V            A  +G+ S       P 
Sbjct: 386 SVAAQQQEETQRVEDREEGSRPKKEEAAARVPEEPRGHRRLPRAQGEGSGSKEASANGPV 445

Query: 424 --ESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSS--------------SHPK 483
             E+   T P         L    + +L++  FP L  + SS              ++P 
Sbjct: 446 SQEAFPATGPGPVVALSNTL-PPPSPELKEEDFPSLCASTSSCCTAVTPGSVGLALAYPG 505

Query: 484 PMHDKDASQNNSMAAHLRRQRKVTVLNSA------QGWPKSGRAPVLPSN---NSSQAWP 543
           P   K+  Q     A +    K +   S+       G  K G  P   S      SQ   
Sbjct: 506 PPRGKNTFQEEDFPALVSSAPKPSSAPSSLISAWNSGCSKKGNLPTPGSQAVVGGSQPPR 565

Query: 544 AINVNHAASSSSGQAKGVVTISNGPSVSGYSN--------------SAQMHGKALSSSSS 603
                       G A      S G +V G  +              + Q   K       
Sbjct: 566 KAGKGSRGGRKGGPAPVDEEDSGGLTVQGLRSVPTTVAVSSLLAPATNQSSAKVGKKKKV 625

Query: 604 GSSSSGQTKG----------------------------VVTISNG--------------- 663
           GS   G T                              V  + NG               
Sbjct: 626 GSEKPGATSSPLLPPDHTPKPSGAEQVLEAPLSKAEVPVTIVVNGHSEGSALVRSAPKEP 685

Query: 664 ------------------------------PSVSGYSNAAQVQGKGR------------S 684
                                         P   G+S    ++G               S
Sbjct: 686 PGLPRPLGPLPCPIPQEDFPALGGPCPPRMPPPPGFSTVVLLKGTPPPPPPPPGLVPPIS 745

BLAST of Moc09g05900 vs. ExPASy TrEMBL
Match: A0A6J1D4L3 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016965 PE=4 SV=1)

HSP 1 Score: 1684.5 bits (4361), Expect = 0.0e+00
Identity = 865/865 (100.00%), Postives = 865/865 (100.00%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD
Sbjct: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM
Sbjct: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420

Query: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480
           HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS
Sbjct: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480

Query: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540
           SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN
Sbjct: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540

Query: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600
           AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ
Sbjct: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600

Query: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660
           SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS
Sbjct: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720
           HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA
Sbjct: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720

Query: 721 KDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQ 780
           KDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQ
Sbjct: 721 KDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGGQ 780

Query: 781 NSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVER 840
           NSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVER
Sbjct: 781 NSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPVER 840

Query: 841 IDDNNSGGGVPVRGVWRNGAQKLFS 866
           IDDNNSGGGVPVRGVWRNGAQKLFS
Sbjct: 841 IDDNNSGGGVPVRGVWRNGAQKLFS 865

BLAST of Moc09g05900 vs. ExPASy TrEMBL
Match: A0A6J1GQ42 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456508 PE=4 SV=1)

HSP 1 Score: 1399.8 bits (3622), Expect = 0.0e+00
Identity = 730/871 (83.81%), Postives = 763/871 (87.60%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCAD LEWVAYGSCGHRDVCSTCVARLRFICGDR CCICKSES VVFVTKALGD
Sbjct: 1   MDDSCAVCADALEWVAYGSCGHRDVCSTCVARLRFICGDRCCCICKSESTVVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGR GSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRCGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PNDG KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCE+CRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEYCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQAHFLCEDE CL+KKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDETCLSKKFIVFQSESEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLS+AIQ SFET N DDT HDPLP     SGQV SD
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSVAIQESFETANVDDTTHDPLP-----SGQVDSD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           QGNISNIDPLIEPFESL+TTD ESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP+
Sbjct: 361 QGNISNIDPLIEPFESLSTTDAESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPI 420

Query: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480
            DKD   NNSMAAHLRR+R VTV+N AQGWPKS RAPV+PSN SSQAWPAINVNH ASSS
Sbjct: 421 QDKD---NNSMAAHLRRKRNVTVINPAQGWPKSSRAPVVPSNKSSQAWPAINVNHTASSS 480

Query: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540
           SGQ KG VTISNG S SGYSN+AQMH K  +SSSSGS S                     
Sbjct: 481 SGQTKGAVTISNGSSGSGYSNAAQMHVKGKTSSSSGSGS--------------------- 540

Query: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600
                            SSSRISHSASAP+LTD+AY EPSVNDFPPVSAAQARK PSSSQ
Sbjct: 541 -----------------SSSRISHSASAPNLTDMAYTEPSVNDFPPVSAAQARKVPSSSQ 600

Query: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660
           SS+N EDVQTANKSLVEKIRAALDFDQDRYA+FKDISAQYRQG I+ +MYLDCVQQFGLS
Sbjct: 601 SSMNTEDVQTANKSLVEKIRAALDFDQDRYAIFKDISAQYRQGQIDPEMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720
           HLVLELARLCPDAQKQKELV  YNASLQK+VIQENGRAQD IQIKDK KGKKGKGK  D 
Sbjct: 661 HLVLELARLCPDAQKQKELVGIYNASLQKEVIQENGRAQDSIQIKDKDKGKKGKGKSTDV 720

Query: 721 KD----TSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGG 780
           KD     SSKD+LAD ILSSVRELQS+  P EE+VEVLSKGEYRA KGK+K+SSD+QQ G
Sbjct: 721 KDRSSKDSSKDRLADGILSSVRELQSSLRPLEEDVEVLSKGEYRALKGKTKISSDDQQSG 780

Query: 781 TGGQNSQPSTGLSNQNNTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDP 840
           TGGQN Q STGLSN  +TGDG GGGSKQ+KKTSKFHRVRLGDGSVAALLDLKN NPASD 
Sbjct: 781 TGGQNRQTSTGLSNHQSTGDGDGGGSKQRKKTSKFHRVRLGDGSVAALLDLKNSNPASDS 825

Query: 841 DPVERIDD-NNSGGGVPVRGVWRNGAQKLFS 866
           DP ER++D NN+ G VPVRGVWRNGAQ+LFS
Sbjct: 841 DPSERVEDGNNTAGAVPVRGVWRNGAQRLFS 825

BLAST of Moc09g05900 vs. ExPASy TrEMBL
Match: A0A0A0K864 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G419600 PE=4 SV=1)

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 724/869 (83.31%), Postives = 766/869 (88.15%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEP+EGR GSYWYHEDTQAFFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRCGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PND  KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRK+FICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQ HFLCEDEACLAKKF+VFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEACLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLSLA+Q SFET N DD NHDPLP     SGQVASD
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSLALQESFETANVDDNNHDPLP-----SGQVASD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           Q N+SN+DPLIE FE+LATTDPESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP 
Sbjct: 361 QENLSNVDPLIESFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPN 420

Query: 421 HDKDASQNNSMAAHLRRQR-KVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASS 480
            DKD   NNSMAAHLRRQR  VTVLNSA GWPKS RAPVLPSNNSSQAWPAIN NHAASS
Sbjct: 421 QDKDIIHNNSMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHAASS 480

Query: 481 SSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYS 540
           SSGQ KGV TI+NGPSVS Y+N+AQMH K                               
Sbjct: 481 SSGQTKGVATINNGPSVSAYANAAQMHPKP------------------------------ 540

Query: 541 NAAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSS 600
                    RS+SSSG GSSSRISHSASAP+LTDIA+ EPSVN+FPPVSAA ARK PSSS
Sbjct: 541 ---------RSTSSSGSGSSSRISHSASAPNLTDIAHTEPSVNEFPPVSAAHARKVPSSS 600

Query: 601 QSSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGL 660
           QSS+N+EDVQTANKSLVEKIRAALDFDQDRY++FKDISAQYRQG I+T+MYLDCVQQFGL
Sbjct: 601 QSSMNMEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQGQIDTEMYLDCVQQFGL 660

Query: 661 SHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYID 720
           SHL+LELARLCPD QKQKELVETYNAS  KDV   NGRAQD IQIKDKSKGKKGKGK I+
Sbjct: 661 SHLLLELARLCPDPQKQKELVETYNASFHKDVFPVNGRAQDSIQIKDKSKGKKGKGKSIE 720

Query: 721 AKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGG 780
            KD+SSKDKLADSI+SSVRELQS++ P +E+VEVLSKGEYR +KGK K+SSD+QQGGTG 
Sbjct: 721 VKDSSSKDKLADSIMSSVRELQSSYRPPDEDVEVLSKGEYRTSKGKLKISSDDQQGGTGR 780

Query: 781 QNSQPSTGLSNQN--NTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDP 840
           Q SQPSTGLSNQ+  + G GGGGSKQKKKTSKFHRVRLGDGSVAALLDLKN N  SDPDP
Sbjct: 781 QKSQPSTGLSNQSTGDGGGGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNLGSDPDP 824

Query: 841 VERIDDNNSG-GGVPVRGVWRNGAQKLFS 866
            ER++D N+G G +PVRGVWRNGAQKLFS
Sbjct: 841 DERVEDRNNGAGALPVRGVWRNGAQKLFS 824

BLAST of Moc09g05900 vs. ExPASy TrEMBL
Match: A0A5D3CF15 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00030 PE=4 SV=1)

HSP 1 Score: 1395.2 bits (3610), Expect = 0.0e+00
Identity = 725/868 (83.53%), Postives = 764/868 (88.02%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFIC DRRCCICKSESAVVFV+KALGD
Sbjct: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICSDRRCCICKSESAVVFVSKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEP+EGR+GSYWYHEDTQAFFDD DHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPKEGRSGSYWYHEDTQAFFDDADHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PND  KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRK+FICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDASKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKVFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQ HFLCEDE CLAKKF+VFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQGHFLCEDEGCLAKKFVVFQSEAEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQ SFET N DD NHDPLP     SGQVASD
Sbjct: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQESFETANVDDNNHDPLP-----SGQVASD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           Q N+SN+DPLIEPFE+LATTDPESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP 
Sbjct: 361 QENVSNVDPLIEPFEALATTDPESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPN 420

Query: 421 HDKDASQNNSMAAHLRRQR-KVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASS 480
            DKD     SMAAHLRRQR  VTVLNSA GWPKS RAPVLPSNNSSQAWPAIN NH+ASS
Sbjct: 421 QDKD-----SMAAHLRRQRNNVTVLNSA-GWPKSSRAPVLPSNNSSQAWPAINSNHSASS 480

Query: 481 SSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYS 540
           SSGQ KGV TI+NGPSVS Y+N+AQMH K                               
Sbjct: 481 SSGQTKGVATINNGPSVSAYANAAQMHPKP------------------------------ 540

Query: 541 NAAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSS 600
                    R SSSSG GSSSRISHSASAP+LTDIA+ EPS+N+FPPVSAAQARK PSSS
Sbjct: 541 ---------RCSSSSGSGSSSRISHSASAPNLTDIAHTEPSLNEFPPVSAAQARKVPSSS 600

Query: 601 QSSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGL 660
           QSS+NVEDVQTANKSLVEKIRAALDFDQDRY++FKDISAQYRQG I+T+MYLDCVQQFGL
Sbjct: 601 QSSMNVEDVQTANKSLVEKIRAALDFDQDRYSIFKDISAQYRQGQIDTEMYLDCVQQFGL 660

Query: 661 SHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYID 720
           SHL+LELARLCPD QKQKELVETYNAS  KDVI  NGRAQD IQIKDKSKGKKGKGK I+
Sbjct: 661 SHLLLELARLCPDPQKQKELVETYNASFHKDVIPVNGRAQDSIQIKDKSKGKKGKGKSIE 720

Query: 721 AKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGGTGG 780
            KD+SSKDKLADSILSSVRELQS++ PSEE+VEVLSKGEYR +KGK K+SSD+QQGGTGG
Sbjct: 721 VKDSSSKDKLADSILSSVRELQSSYRPSEEDVEVLSKGEYRTSKGKLKISSDDQQGGTGG 780

Query: 781 QNSQPSTGLSNQN-NTGDGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDPDPV 840
           Q SQPSTGLSNQ+   G GGGGSKQKKKTSKFHRVRLGDGSVAALLDLKN N  SDPDP 
Sbjct: 781 QKSQPSTGLSNQSTGDGGGGGGSKQKKKTSKFHRVRLGDGSVAALLDLKNSNLTSDPDPD 818

Query: 841 ERIDD-NNSGGGVPVRGVWRNGAQKLFS 866
           E ++D NN+ G +PVRGVWRNGA +LFS
Sbjct: 841 ESVEDRNNAAGALPVRGVWRNGAHRLFS 818

BLAST of Moc09g05900 vs. ExPASy TrEMBL
Match: A0A6J1JVL7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488249 PE=4 SV=1)

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 727/871 (83.47%), Postives = 766/871 (87.94%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCAD LEWVAYGSCGHRDVCSTCVARLRFICGDR CCICKSES VVFVTKALGD
Sbjct: 1   MDDSCAVCADALEWVAYGSCGHRDVCSTCVARLRFICGDRCCCICKSESTVVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDFTVFPSEPREGR GSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ
Sbjct: 61  YTRTINDFTVFPSEPREGRCGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120

Query: 121 PNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTGD 180
           PNDG KRRG+FRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLY+RAQLNQHI+TGD
Sbjct: 121 PNDGSKRRGRFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYNRAQLNQHIHTGD 180

Query: 181 SEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240
           SEVDGSESERGGFTGHPMCE+CRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY
Sbjct: 181 SEVDGSESERGGFTGHPMCEYCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKNY 240

Query: 241 DDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300
           DDLEIHFRQAHFLCEDEACL+KKFIVFQSE EMKRHNTIEHGGKLSRSKRNAALQIPTSF
Sbjct: 241 DDLEIHFRQAHFLCEDEACLSKKFIVFQSESEMKRHNTIEHGGKLSRSKRNAALQIPTSF 300

Query: 301 RYRRSNEQDNRRGRRTFRRDSSDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQVASD 360
           RYRRSN+QDNRRGRRTFRRDSSDDLLS+AIQ SFET N DDT HDPLP     SGQV SD
Sbjct: 301 RYRRSNDQDNRRGRRTFRRDSSDDLLSVAIQESFETANVDDTTHDPLP-----SGQVDSD 360

Query: 361 QGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHPKPM 420
           QGNISNIDPLIEPFE+L+TTD ESASRYLQALGHSRNSQLEQSSFPPLSTA SSSHPKP+
Sbjct: 361 QGNISNIDPLIEPFEALSTTDSESASRYLQALGHSRNSQLEQSSFPPLSTASSSSHPKPI 420

Query: 421 HDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAASSS 480
            DKD   NNSMAAHLRR+R VTV+N AQGWPKS RAPV+PSN SSQAWPAINVNH ASSS
Sbjct: 421 PDKD---NNSMAAHLRRKRNVTVINPAQGWPKSSRAPVVPSNKSSQAWPAINVNHTASSS 480

Query: 481 SGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSGYSN 540
           SGQ KG VTISNG S SGYSN+AQMH K  ++SSSG  S                     
Sbjct: 481 SGQTKGAVTISNGSSGSGYSNAAQMHAKGKTNSSSGPGS--------------------- 540

Query: 541 AAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAAQARKAPSSSQ 600
                            SSSRISHSASAP+LTD+AY EPSVNDFPPVSAAQARK PSSSQ
Sbjct: 541 -----------------SSSRISHSASAPNLTDMAYTEPSVNDFPPVSAAQARKVPSSSQ 600

Query: 601 SSLNVEDVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQMYLDCVQQFGLS 660
           SS+N EDVQTANKSLVEKIRAALDFDQDRYA+FKDISAQYRQG I+ +MYLDCVQQFGLS
Sbjct: 601 SSMNTEDVQTANKSLVEKIRAALDFDQDRYAIFKDISAQYRQGQIDPEMYLDCVQQFGLS 660

Query: 661 HLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSKGKKGKGKYIDA 720
           HLVLELARLCPDAQKQKELV  YNASLQ++VIQENGRAQD IQI  K KGKKGKGK ID 
Sbjct: 661 HLVLELARLCPDAQKQKELVGIYNASLQQEVIQENGRAQDSIQI--KVKGKKGKGKSIDV 720

Query: 721 KDT----SSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLSSDEQQGG 780
           KD+    SSKD+LADSILSSVRELQS+  P EE+VEVLSKGEYRA KGK+K+SSD+QQ G
Sbjct: 721 KDSSSKDSSKDRLADSILSSVRELQSSLRPLEEDVEVLSKGEYRALKGKTKISSDDQQSG 780

Query: 781 TGGQNSQPSTGLSNQNNTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKNPNPASDP 840
           TGGQN QPSTGLSN ++TGDG GGG+KQ+KKTSKFHRVRLGDGSVAALLDLKN NPASD 
Sbjct: 781 TGGQNRQPSTGLSNHHSTGDGDGGGTKQRKKTSKFHRVRLGDGSVAALLDLKNSNPASDS 823

Query: 841 DPVERIDD-NNSGGGVPVRGVWRNGAQKLFS 866
           DP ER++D NN+ G VPVRGVWRNGAQ+LFS
Sbjct: 841 DPSERVEDGNNTAGAVPVRGVWRNGAQRLFS 823

BLAST of Moc09g05900 vs. TAIR 10
Match: AT3G62240.1 (RING/U-box superfamily protein )

HSP 1 Score: 838.2 bits (2164), Expect = 6.0e-243
Identity = 482/884 (54.52%), Postives = 603/884 (68.21%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCAD LEWVAYGSCGHR+VCSTCV RLRF+  D RCCICK+ES +VFVTKALGD
Sbjct: 1   MDDSCAVCADNLEWVAYGSCGHREVCSTCVVRLRFVLDDPRCCICKTESPIVFVTKALGD 60

Query: 61  YTRTINDFTVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGEDQ 120
           YTRTINDF+ FPS PREGR G++WYHEDTQAFFDD+D Y+MIKAMCRLSC VCDK  ED+
Sbjct: 61  YTRTINDFSTFPSAPREGRVGAFWYHEDTQAFFDDLDQYRMIKAMCRLSCGVCDKT-EDK 120

Query: 121 PNDGPKR--RGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINT 180
           P +G  R  R + +++EQLKGHL+H+HKL MC LCLEGRKIFICEQKLY+RAQLNQHI T
Sbjct: 121 PREGGPRHHRQRIKSVEQLKGHLYHKHKLHMCGLCLEGRKIFICEQKLYTRAQLNQHIQT 180

Query: 181 GDSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYK 240
           GDSEVDGSESERGGF GHPMCEFCR PFYGDNELYTHMSTEHYTCH+CQR  PGQYEYYK
Sbjct: 181 GDSEVDGSESERGGFAGHPMCEFCRNPFYGDNELYTHMSTEHYTCHLCQRSQPGQYEYYK 240

Query: 241 NYDDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPT 300
           NYDDLEIHFR+ HFLCED++CLAKKF VFQ+E E+KRHN IEHGGK+SRS+R+AALQIPT
Sbjct: 241 NYDDLEIHFRRDHFLCEDDSCLAKKFTVFQNESELKRHNAIEHGGKMSRSQRSAALQIPT 300

Query: 301 SFRYRRSNEQDNRRGR-RTFRRDSSDDLLSLAIQASFETGNADDTNHDPL-PSSSFSSGQ 360
           SFRY R N+Q+NRRGR R+FRR+  D+  +LA+ A+     ++ +  +P  P SS   G 
Sbjct: 301 SFRYSRGNDQENRRGRPRSFRREPGDEEYNLAVHAALRLSESEYSRQEPAPPPSSAPPGF 360

Query: 361 VASDQGNISNIDPLIEPFESLATTDPESASRYLQALGH--SRNSQLEQSSFPPLSTAPSS 420
             ++  ++ + DPLI+P ESL+TTD E +SRYLQA+G      S+L +S+FPPLS   SS
Sbjct: 361 SENNNIHVDDTDPLIQPMESLSTTDMEPSSRYLQAVGSFGGGGSRLGESAFPPLSGQQSS 420

Query: 421 SHPKPMHDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVN 480
                  + ++   N+MAA LRRQ   T   SA   P             SQ WP IN  
Sbjct: 421 G-----QNVESLPTNTMAARLRRQTNRTSTASAIASP-------------SQGWPVIN-- 480

Query: 481 HAASSSSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISN-GP 540
                  G  +  +T     S SG+    +   +A       SSSS Q++    +S   P
Sbjct: 481 ------RGPGQASITSGGNHSSSGWPAIGRTPVQA-------SSSSVQSRSHNRVSQPRP 540

Query: 541 SVSGYSNAAQVQGKGRSSSSSGLGSSSRISHSASAPSLTDIAYAEPSVNDFPPVSAA--Q 600
             S    AA+              +++RI HS+SAP+L+D    +PS +DFPPVS+A  Q
Sbjct: 541 LASAVPQAAR--------------NANRIPHSSSAPNLSDTRSLQPSHSDFPPVSSAVVQ 600

Query: 601 ARKAPS-SSQSSLNVE---DVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIET 660
            RK  S ++Q S N +   DVQ+ANKSL+EK+R+AL  D+D +  F++IS QYRQG I+ 
Sbjct: 601 NRKTSSTTTQGSSNTQPPPDVQSANKSLIEKMRSALGHDEDVFVAFRNISGQYRQGSIDA 660

Query: 661 QMYLDCVQQFGLSHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGR--AQDGIQIK 720
           + YL+ VQ +GLSHLV++LARLCPD ++QKEL++T+NASL+++  ++NGR  AQ   Q K
Sbjct: 661 KTYLEYVQGYGLSHLVIDLARLCPDPKRQKELIDTHNASLREEDSKDNGRSAAQSSSQPK 720

Query: 721 DKSKGKKGKGKYIDAKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGE--YRAAK 780
           +    KK KGK +   D   K+ LAD+ + +VR LQS+  P EEE E +SK +  YR+ K
Sbjct: 721 ESQSSKKNKGKAVKVVD--PKETLADNFMDTVRRLQSSQNPQEEEEEAISKDKNTYRSDK 780

Query: 781 GKSKLSSDEQQGGTGGQNSQPSTGLSNQNNTGDGGGGSKQKKKTSKFHRVRLGDGSVAAL 840
           GKS      Q  GT   ++                G  +Q+KKTSKFHRVRLGDGS+AAL
Sbjct: 781 GKS------QVVGTDSSST----------------GSKQQRKKTSKFHRVRLGDGSMAAL 812

Query: 841 LDLKNPNPASDPDPVERIDDNNSG--GGVPVRGVWRNGAQKLFS 866
           LDL N    S+ +  +   ++N    GG+PVRGVWR G   LFS
Sbjct: 841 LDLNNSTRESEQESKDSNSNSNQNQTGGLPVRGVWRKGGANLFS 812

BLAST of Moc09g05900 vs. TAIR 10
Match: AT2G47090.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 652.1 bits (1681), Expect = 6.1e-187
Identity = 405/881 (45.97%), Postives = 533/881 (60.50%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCA+ LEWV YGSCGHR+VCSTCV RLRFI  DRRCCICK+E  VVFVTKALGD
Sbjct: 1   MDDSCAVCAENLEWVGYGSCGHREVCSTCVVRLRFILNDRRCCICKTECPVVFVTKALGD 60

Query: 61  YTRTINDF-TVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGED 120
           YT+TI+DF T FPS P+EGR GS+WYHE+T  +FDD++HY  IKAMCRLSC++C+   + 
Sbjct: 61  YTKTISDFSTTFPSVPKEGRVGSFWYHEETNVYFDDLNHYTRIKAMCRLSCNLCNDTNKT 120

Query: 121 QPNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTG 180
           +P   P    +F+++E LK HL H+HKL MCSLCL GRK+FICEQKL+++ QLNQHI++G
Sbjct: 121 RPKKEPNHCVRFKSVEHLKDHLNHQHKLHMCSLCLVGRKVFICEQKLFTKGQLNQHISSG 180

Query: 181 DSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKN 240
           DSEVDGSESERGGFTGHPMCEFC+ PFYGDNELYTHMS EHYTCHICQRL PGQYEYY N
Sbjct: 181 DSEVDGSESERGGFTGHPMCEFCKRPFYGDNELYTHMSREHYTCHICQRLKPGQYEYYGN 240

Query: 241 YDDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTS 300
           YDDLE+HFR  HFLCEDE CLAKKFIVFQ E E+KRHN I+HGG++SRS++NA+LQI  S
Sbjct: 241 YDDLEVHFRSDHFLCEDETCLAKKFIVFQIEAELKRHNAIDHGGRMSRSQQNASLQIQAS 300

Query: 301 FRYRRSNEQDNRRGRRTFRRDS--SDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQV 360
           F+Y  S     RRGR   RR S    +L+ L  QAS+     DD N   LP         
Sbjct: 301 FQYPNS-----RRGR---RRSSLREPNLVLLESQASYAFN--DDNN---LP--------- 360

Query: 361 ASDQGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHP 420
                                           Q +G S NS+L +SSFPPLS   +    
Sbjct: 361 --------------------------------QHVGRSGNSRLGESSFPPLSVQANQGQS 420

Query: 421 KPMHDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAA 480
           +   + ++  +N+     R +       + QG  + G+      +N++        N +A
Sbjct: 421 RFGQNSESLVSNTTTTRQRHR-------ANQGQSRFGQNSESLVSNTTTTRQRHQTNRSA 480

Query: 481 SSSSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSG 540
           +S S QA     ++ GP+    ++  Q      SS +S  S S     V +     S   
Sbjct: 481 TSGSSQA--WPALNRGPAEISITSRVQ------SSGASAQSQSRHHDRVESTRTLASAVP 540

Query: 541 YSNAAQVQGKGRSSSSSGLGSSSRIS-HSASAPSLTDI-AYAEPSVNDFPPVSAAQARKA 600
                 V G    SS S   ++ R + HS+S P +++  + A+PS +D P +SA + R++
Sbjct: 541 QDARTTVGGCSSGSSLSSANATKRNNHHSSSTPKMSETRSLAQPSHSDSPQISAVKNRRS 600

Query: 601 PSSSQSSLNVE------DVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQM 660
            S+S ++ N++      DVQ+ NKSLVEKI A+L  D++ +  FK+ S +YR G I+ + 
Sbjct: 601 SSTSANAGNIQVAQGVSDVQSDNKSLVEKIHASLGHDEELFMAFKNTSGKYRHGSIDART 660

Query: 661 YLDCVQQFGLSHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSK 720
           YL+ V+ +GLSHLVL++ARLCPD Q+QKEL++T+NA L+                     
Sbjct: 661 YLEYVKGYGLSHLVLDMARLCPDPQRQKELIDTHNACLK--------------------G 720

Query: 721 GKKGKGKYIDAKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLS 780
           G KGK      K  SS D   D  + +VR+LQ +    +++ +   K  YR+ KGK+K+ 
Sbjct: 721 GNKGKA----VKVESSSDSKGDRFVDTVRKLQFSDKSQDKDKD---KDAYRSDKGKTKV- 765

Query: 781 SDEQQGGTGGQNSQPSTGLSNQNNTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKN 840
                           T L N ++ G G G   KQ KKTSKF R RLG+ S+AA+LDL+N
Sbjct: 781 ----------------TTLVNSSSAGVGLGDTGKQPKKTSKFLRTRLGEKSMAAVLDLRN 765

Query: 841 PNPASDPDPVERIDDN-----NSGGGVPVRGVWRNGAQKLF 865
            NP  +P+P    +DN     NS GG+P+RG W+ G+ KLF
Sbjct: 841 SNPEPEPEPK---NDNSKRSQNSPGGLPLRGAWKRGSAKLF 765

BLAST of Moc09g05900 vs. TAIR 10
Match: AT2G47090.2 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 602.1 bits (1551), Expect = 7.3e-172
Identity = 390/881 (44.27%), Postives = 514/881 (58.34%), Query Frame = 0

Query: 1   MDDSCAVCADTLEWVAYGSCGHRDVCSTCVARLRFICGDRRCCICKSESAVVFVTKALGD 60
           MDDSCAVCA+ LEWV YGSCGHR+VCSTCV RLRFI  DRRCCICK+E  VVFVTKALGD
Sbjct: 1   MDDSCAVCAENLEWVGYGSCGHREVCSTCVVRLRFILNDRRCCICKTECPVVFVTKALGD 60

Query: 61  YTRTINDF-TVFPSEPREGRAGSYWYHEDTQAFFDDVDHYKMIKAMCRLSCSVCDKIGED 120
           YT+TI+DF T FPS P+EGR GS+W                       LSC++C+   + 
Sbjct: 61  YTKTISDFSTTFPSVPKEGRVGSFW-----------------------LSCNLCNDTNKT 120

Query: 121 QPNDGPKRRGKFRNIEQLKGHLFHRHKLFMCSLCLEGRKIFICEQKLYSRAQLNQHINTG 180
           +P   P    +F+++E LK HL H+HKL MCSLCL GRK+FICEQKL+++ QLNQHI++G
Sbjct: 121 RPKKEPNHCVRFKSVEHLKDHLNHQHKLHMCSLCLVGRKVFICEQKLFTKGQLNQHISSG 180

Query: 181 DSEVDGSESERGGFTGHPMCEFCRTPFYGDNELYTHMSTEHYTCHICQRLHPGQYEYYKN 240
           DSEVDGSESERGGFTGHPMCEFC+ PFYGDNELYTHMS EHYTCHICQRL PGQYEYY N
Sbjct: 181 DSEVDGSESERGGFTGHPMCEFCKRPFYGDNELYTHMSREHYTCHICQRLKPGQYEYYGN 240

Query: 241 YDDLEIHFRQAHFLCEDEACLAKKFIVFQSEGEMKRHNTIEHGGKLSRSKRNAALQIPTS 300
           YDDLE+HFR  HFLCEDE CLAKKFIVFQ E E+KRHN I+HGG++SRS++NA+LQI  S
Sbjct: 241 YDDLEVHFRSDHFLCEDETCLAKKFIVFQIEAELKRHNAIDHGGRMSRSQQNASLQIQAS 300

Query: 301 FRYRRSNEQDNRRGRRTFRRDS--SDDLLSLAIQASFETGNADDTNHDPLPSSSFSSGQV 360
           F+Y  S     RRGR   RR S    +L+ L  QAS+     DD N   LP         
Sbjct: 301 FQYPNS-----RRGR---RRSSLREPNLVLLESQASYAFN--DDNN---LP--------- 360

Query: 361 ASDQGNISNIDPLIEPFESLATTDPESASRYLQALGHSRNSQLEQSSFPPLSTAPSSSHP 420
                                           Q +G S NS+L +SSFPPLS   +    
Sbjct: 361 --------------------------------QHVGRSGNSRLGESSFPPLSVQANQGQS 420

Query: 421 KPMHDKDASQNNSMAAHLRRQRKVTVLNSAQGWPKSGRAPVLPSNNSSQAWPAINVNHAA 480
           +   + ++  +N+     R +       + QG  + G+      +N++        N +A
Sbjct: 421 RFGQNSESLVSNTTTTRQRHR-------ANQGQSRFGQNSESLVSNTTTTRQRHQTNRSA 480

Query: 481 SSSSGQAKGVVTISNGPSVSGYSNSAQMHGKALSSSSSGSSSSGQTKGVVTISNGPSVSG 540
           +S S QA     ++ GP+    ++  Q      SS +S  S S     V +     S   
Sbjct: 481 TSGSSQA--WPALNRGPAEISITSRVQ------SSGASAQSQSRHHDRVESTRTLASAVP 540

Query: 541 YSNAAQVQGKGRSSSSSGLGSSSRIS-HSASAPSLTDI-AYAEPSVNDFPPVSAAQARKA 600
                 V G    SS S   ++ R + HS+S P +++  + A+PS +D P +SA + R++
Sbjct: 541 QDARTTVGGCSSGSSLSSANATKRNNHHSSSTPKMSETRSLAQPSHSDSPQISAVKNRRS 600

Query: 601 PSSSQSSLNVE------DVQTANKSLVEKIRAALDFDQDRYAVFKDISAQYRQGLIETQM 660
            S+S ++ N++      DVQ+ NKSLVEKI A+L  D++ +  FK+ S +YR G I+ + 
Sbjct: 601 SSTSANAGNIQVAQGVSDVQSDNKSLVEKIHASLGHDEELFMAFKNTSGKYRHGSIDART 660

Query: 661 YLDCVQQFGLSHLVLELARLCPDAQKQKELVETYNASLQKDVIQENGRAQDGIQIKDKSK 720
           YL+ V+ +GLSHLVL++ARLCPD Q+QKEL++T+NA L+                     
Sbjct: 661 YLEYVKGYGLSHLVLDMARLCPDPQRQKELIDTHNACLK--------------------G 720

Query: 721 GKKGKGKYIDAKDTSSKDKLADSILSSVRELQSNFMPSEEEVEVLSKGEYRAAKGKSKLS 780
           G KGK      K  SS D   D  + +VR+LQ +    +++ +   K  YR+ KGK+K+ 
Sbjct: 721 GNKGKA----VKVESSSDSKGDRFVDTVRKLQFSDKSQDKDKD---KDAYRSDKGKTKV- 742

Query: 781 SDEQQGGTGGQNSQPSTGLSNQNNTGDG-GGGSKQKKKTSKFHRVRLGDGSVAALLDLKN 840
                           T L N ++ G G G   KQ KKTSKF R RLG+ S+AA+LDL+N
Sbjct: 781 ----------------TTLVNSSSAGVGLGDTGKQPKKTSKFLRTRLGEKSMAAVLDLRN 742

Query: 841 PNPASDPDPVERIDDN-----NSGGGVPVRGVWRNGAQKLF 865
            NP  +P+P    +DN     NS GG+P+RG W+ G+ KLF
Sbjct: 841 SNPEPEPEPK---NDNSKRSQNSPGGLPLRGAWKRGSAKLF 742

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148262.10.0e+00100.00E3 ubiquitin-protein ligase HEL2 [Momordica charantia][more]
XP_038897973.10.0e+0084.91E3 ubiquitin-protein ligase HEL2 [Benincasa hispida][more]
XP_023547810.10.0e+0083.58E3 ubiquitin-protein ligase HEL2 [Cucurbita pepo subsp. pepo][more]
XP_022954166.10.0e+0083.81E3 ubiquitin-protein ligase HEL2 [Cucurbita moschata][more]
XP_004135963.10.0e+0083.31E3 ubiquitin-protein ligase ZNF598 [Cucumis sativus] >KGN45064.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q76PD22.1e-3830.99E3 ubiquitin-protein ligase hel2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q86UK72.6e-3327.98E3 ubiquitin-protein ligase ZNF598 OS=Homo sapiens OX=9606 GN=ZNF598 PE=1 SV=1[more]
Q6PFK19.3e-3124.95E3 ubiquitin-protein ligase ZNF598 OS=Danio rerio OX=7955 GN=znf598 PE=1 SV=2[more]
Q055801.3e-2925.34E3 ubiquitin-protein ligase HEL2 OS=Saccharomyces cerevisiae (strain ATCC 204508... [more]
Q80YR41.3e-2423.25E3 ubiquitin-protein ligase ZNF598 OS=Mus musculus OX=10090 GN=Znf598 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D4L30.0e+00100.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101696... [more]
A0A6J1GQ420.0e+0083.81RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456508... [more]
A0A0A0K8640.0e+0083.31RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G419600 PE... [more]
A0A5D3CF150.0e+0083.53RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1JVL70.0e+0083.47RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488249 P... [more]
Match NameE-valueIdentityDescription
AT3G62240.16.0e-24354.52RING/U-box superfamily protein [more]
AT2G47090.16.1e-18745.97zinc ion binding;nucleic acid binding [more]
AT2G47090.27.3e-17244.27zinc ion binding;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 108..145
e-value: 64.0
score: 6.2
coord: 197..220
e-value: 15.0
score: 11.8
coord: 252..281
e-value: 84.0
score: 5.1
coord: 221..251
e-value: 14.0
score: 12.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 698..719
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 498..564
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 592..606
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..434
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 700..719
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 336..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 587..606
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 762..865
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..416
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 768..801
NoneNo IPR availablePANTHERPTHR22938:SF14EBR1coord: 1..863
IPR044288E3 ubiquitin-protein ligase ZNF598/Hel2PANTHERPTHR22938ZINC FINGER PROTEIN 598coord: 1..863
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 5..46
score: 9.214112
IPR041888ZN598/Hel2, RING fingerCDDcd16615RING-HC_ZNF598coord: 5..46
e-value: 3.81231E-16
score: 70.7739

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g05900.1Moc09g05900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0072344 rescue of stalled ribosome
molecular_function GO:0046872 metal ion binding
molecular_function GO:0043022 ribosome binding
molecular_function GO:0061630 ubiquitin protein ligase activity