Moc09g05860 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g05860
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Delta(24)-sterol reductase
Location	chr9: 4530239 .. 4532239 (-)
RNA-Seq Expression	Moc09g05860
Synteny	Moc09g05860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGATCTACAGACTCCTCTGCGCCGAAAGAGGAAGAAGGTCTGGGTGGACTACTTCGTCCAATTTAGATGGATTTTAGTTGTTTTTGTTGTTCTTCCCATATCATTCAGCCTATACTTCTTCACATATCTTGGAGACGTCAGATCTGCTTGGAAGTCCTACAAGAAACGCCAGAAGCAACACGATGAAAATGTCAAGAAAGTTGTGAAGCGCCTGAAACAGAGGAACCCAGCCAAGGATGGCCTAGTTTGCACAGCTCGAAAGCCCTGGATCGCAGTTGGAATGAGAAATGTTGACTATAAACGGGCACGCCACTTCGAGGTTGATCTATCAGCCTTCCGAAACATTCTGGAAATTGACCAACAAAGAATGATTGCCAAAGTTGAGCCCCTTGTGAACATGGGCCAAATAAGTAGAGCCACTGTTCCACTGAATCTCTCTCTAGCCGTGGTTGCTGAACTTGATGATCTTACTGTCGGGGGACTCATCAATGGCTATGGTATAGAAGGAAGCTCCCATCTCTACGGCTTGTTCTCCGACACGGTTGTCGCATATGAGATTGTCCTAGCAGACGGCCGGGTTGTCAGAGCTACCAAAGATAACAAGTACTCGGATCTTTTCTATGCAATCCCATGGTCCCAGGGGACAATTGGCCTTCTAGTCGCTGCCGAGATCAAGCTTATACCAATTAAAGAGTATATGAGAGTTACATATAAACCATACATAGGCACTTTAAGAGATATAGGACAGGCTTACATGGATTCTCTCGCACCTAGAGATGGAGATCAAGACAATCCAGAGAAGGTTCCTGATTTTGTAGAAACTATGGTTTATACTCCTTCGGAAGCTGTGATCATGTCAGGTAGATATGCTTCTAAAGTGGAGGCTAAGAAAAGGGGAAATGTGATCAATAATATCGGGTGGTGGTTCAAACCATGGTTCTACCAGCATGCAGCTACGGCACTGAAGAGAGGGGAGTTTGTGGAGTATATTCCTACAAGGGAGTACTATCATAGGCACACCAGGTCTTTGTATTGGGAGGGAAAGCTCATTCTTCCATTTGCAGATCAGTGGTGGTTCAGGTTTTTCATGGGATGGCTTATGCCTCCCAAAGTTTCTCTGCTGAAAGCTACTCAAGGTGAAGCTATCAGAAACTATTATCACGAAATGCATGTAATTCAAGATATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGCGAGTTGGAGGTATTGATTTTTTCACCTTAAAACTTCTGGTTTTTCTTATATATTTGCTGGAGGTGTGGAGCTAGAAACTTTCATGGGAGGGGCAAGATTTTGCATTCTAACTATTTATGTGCATGTTTTTAGTGTTTGTTTTTATATACCTCTTGTTATTTTTGACTATTGGACAAATAAAGAGCGGGGCAGTGGTAATAGGGAAATGGCTGATGAGCCGTTCACAATTTAGAAGTGGATTTCAATGGAAGTATGAAGTTTTACTTGGTTAACAACAGCAATTTGTTTGTGTGTTCTGCACAGGTTTACCCCATTTGGCTCTGCCCACACAGAATCTTCAAGCTTCCAGTCAAAACCATGATATATCCGGAACCAGGATTTGAGTTTCATCGTCGACAGGGAGACACGCACTACGCGCAGATGTACACAGATGTTGGACTGTACTATGCACCAGGCCCCGTCCTGAGGGGCGAAGTTTTCGATGGTTCGGAGGCTGTCCGAGGATTAGAGAAATGGCTTCTAGAAAACCATGGATTCCAGGCTCTATACGCGGTGTCGGAACTGAATGAGAAAGATTTCTGGAGAATGTTTGATGGTGGACTGTATGAGCAATGCCGGAAGAAGTATGGTGCTGTAGGAACCTTCATGACTGTATACTACAAGTCCAAAAAAGGAAGGAAAACTGAGAAGGAAGTGAGAGAAGCTGAACTTGAACAAGCTCATCATGAAACTACTTATGCAGAATTGGATCAACCACAATAA

mRNA sequence

ATGTCAGATCTACAGACTCCTCTGCGCCGAAAGAGGAAGAAGGTCTGGGTGGACTACTTCGTCCAATTTAGATGGATTTTAGTTGTTTTTGTTGTTCTTCCCATATCATTCAGCCTATACTTCTTCACATATCTTGGAGACGTCAGATCTGCTTGGAAGTCCTACAAGAAACGCCAGAAGCAACACGATGAAAATGTCAAGAAAGTTGTGAAGCGCCTGAAACAGAGGAACCCAGCCAAGGATGGCCTAGTTTGCACAGCTCGAAAGCCCTGGATCGCAGTTGGAATGAGAAATGTTGACTATAAACGGGCACGCCACTTCGAGGTTGATCTATCAGCCTTCCGAAACATTCTGGAAATTGACCAACAAAGAATGATTGCCAAAGTTGAGCCCCTTGTGAACATGGGCCAAATAAGTAGAGCCACTGTTCCACTGAATCTCTCTCTAGCCGTGGTTGCTGAACTTGATGATCTTACTGTCGGGGGACTCATCAATGGCTATGGTATAGAAGGAAGCTCCCATCTCTACGGCTTGTTCTCCGACACGGTTGTCGCATATGAGATTGTCCTAGCAGACGGCCGGGTTGTCAGAGCTACCAAAGATAACAAGTACTCGGATCTTTTCTATGCAATCCCATGGTCCCAGGGGACAATTGGCCTTCTAGTCGCTGCCGAGATCAAGCTTATACCAATTAAAGAGTATATGAGAGTTACATATAAACCATACATAGGCACTTTAAGAGATATAGGACAGGCTTACATGGATTCTCTCGCACCTAGAGATGGAGATCAAGACAATCCAGAGAAGGTTCCTGATTTTGTAGAAACTATGGTTTATACTCCTTCGGAAGCTGTGATCATGTCAGGTAGATATGCTTCTAAAGTGGAGGCTAAGAAAAGGGGAAATGTGATCAATAATATCGGGTGGTGGTTCAAACCATGGTTCTACCAGCATGCAGCTACGGCACTGAAGAGAGGGGAGTTTGTGGAGTATATTCCTACAAGGGAGTACTATCATAGGCACACCAGGTCTTTGTATTGGGAGGGAAAGCTCATTCTTCCATTTGCAGATCAGTGGTGGTTCAGGTTTTTCATGGGATGGCTTATGCCTCCCAAAGTTTCTCTGCTGAAAGCTACTCAAGGTGAAGCTATCAGAAACTATTATCACGAAATGCATGTAATTCAAGATATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGCGAGTTGGAGGTTTACCCCATTTGGCTCTGCCCACACAGAATCTTCAAGCTTCCAGTCAAAACCATGATATATCCGGAACCAGGATTTGAGTTTCATCGTCGACAGGGAGACACGCACTACGCGCAGATGTACACAGATGTTGGACTGTACTATGCACCAGGCCCCGTCCTGAGGGGCGAAGTTTTCGATGGTTCGGAGGCTGTCCGAGGATTAGAGAAATGGCTTCTAGAAAACCATGGATTCCAGGCTCTATACGCGGTGTCGGAACTGAATGAGAAAGATTTCTGGAGAATGTTTGATGGTGGACTGTATGAGCAATGCCGGAAGAAGTATGGTGCTGTAGGAACCTTCATGACTGTATACTACAAGTCCAAAAAAGGAAGGAAAACTGAGAAGGAAGTGAGAGAAGCTGAACTTGAACAAGCTCATCATGAAACTACTTATGCAGAATTGGATCAACCACAATAA

Coding sequence (CDS)

ATGTCAGATCTACAGACTCCTCTGCGCCGAAAGAGGAAGAAGGTCTGGGTGGACTACTTCGTCCAATTTAGATGGATTTTAGTTGTTTTTGTTGTTCTTCCCATATCATTCAGCCTATACTTCTTCACATATCTTGGAGACGTCAGATCTGCTTGGAAGTCCTACAAGAAACGCCAGAAGCAACACGATGAAAATGTCAAGAAAGTTGTGAAGCGCCTGAAACAGAGGAACCCAGCCAAGGATGGCCTAGTTTGCACAGCTCGAAAGCCCTGGATCGCAGTTGGAATGAGAAATGTTGACTATAAACGGGCACGCCACTTCGAGGTTGATCTATCAGCCTTCCGAAACATTCTGGAAATTGACCAACAAAGAATGATTGCCAAAGTTGAGCCCCTTGTGAACATGGGCCAAATAAGTAGAGCCACTGTTCCACTGAATCTCTCTCTAGCCGTGGTTGCTGAACTTGATGATCTTACTGTCGGGGGACTCATCAATGGCTATGGTATAGAAGGAAGCTCCCATCTCTACGGCTTGTTCTCCGACACGGTTGTCGCATATGAGATTGTCCTAGCAGACGGCCGGGTTGTCAGAGCTACCAAAGATAACAAGTACTCGGATCTTTTCTATGCAATCCCATGGTCCCAGGGGACAATTGGCCTTCTAGTCGCTGCCGAGATCAAGCTTATACCAATTAAAGAGTATATGAGAGTTACATATAAACCATACATAGGCACTTTAAGAGATATAGGACAGGCTTACATGGATTCTCTCGCACCTAGAGATGGAGATCAAGACAATCCAGAGAAGGTTCCTGATTTTGTAGAAACTATGGTTTATACTCCTTCGGAAGCTGTGATCATGTCAGGTAGATATGCTTCTAAAGTGGAGGCTAAGAAAAGGGGAAATGTGATCAATAATATCGGGTGGTGGTTCAAACCATGGTTCTACCAGCATGCAGCTACGGCACTGAAGAGAGGGGAGTTTGTGGAGTATATTCCTACAAGGGAGTACTATCATAGGCACACCAGGTCTTTGTATTGGGAGGGAAAGCTCATTCTTCCATTTGCAGATCAGTGGTGGTTCAGGTTTTTCATGGGATGGCTTATGCCTCCCAAAGTTTCTCTGCTGAAAGCTACTCAAGGTGAAGCTATCAGAAACTATTATCACGAAATGCATGTAATTCAAGATATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGCGAGTTGGAGGTTTACCCCATTTGGCTCTGCCCACACAGAATCTTCAAGCTTCCAGTCAAAACCATGATATATCCGGAACCAGGATTTGAGTTTCATCGTCGACAGGGAGACACGCACTACGCGCAGATGTACACAGATGTTGGACTGTACTATGCACCAGGCCCCGTCCTGAGGGGCGAAGTTTTCGATGGTTCGGAGGCTGTCCGAGGATTAGAGAAATGGCTTCTAGAAAACCATGGATTCCAGGCTCTATACGCGGTGTCGGAACTGAATGAGAAAGATTTCTGGAGAATGTTTGATGGTGGACTGTATGAGCAATGCCGGAAGAAGTATGGTGCTGTAGGAACCTTCATGACTGTATACTACAAGTCCAAAAAAGGAAGGAAAACTGAGAAGGAAGTGAGAGAAGCTGAACTTGAACAAGCTCATCATGAAACTACTTATGCAGAATTGGATCAACCACAATAA

Protein sequence

MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQKQHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEIDQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYKPYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKRGNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEAVRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKGRKTEKEVREAELEQAHHETTYAELDQPQ

Homology

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BLAST of Moc09g05860 vs. NCBI nr
Match: XP_022151841.1 (delta(24)-sterol reductase-like [Momordica charantia] >XP_022151850.1 delta(24)-sterol reductase-like [Momordica charantia] >XP_022151858.1 delta(24)-sterol reductase-like [Momordica charantia] >XP_022151867.1 delta(24)-sterol reductase-like [Momordica charantia] >XP_022151876.1 delta(24)-sterol reductase-like [Momordica charantia])

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI
Sbjct: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR
Sbjct: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI
Sbjct: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA
Sbjct: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQPQ 569
           RKTEKEVREAELEQAHHETTYAELDQPQ
Sbjct: 541 RKTEKEVREAELEQAHHETTYAELDQPQ 568

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BLAST of Moc09g05860 vs. NCBI nr
Match: KAG7014278.1 (Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 526/567 (92.77%), Postives = 553/567 (97.53%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFVQFRWILV+FVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D++RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGL+NGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLVNGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADG+VVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+IGTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+MSGRYASK EAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD  LYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETTYAEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTYAELEQP 565

[Close]

BLAST of Moc09g05860 vs. NCBI nr
Match: XP_038898378.1 (delta(24)-sterol reductase [Benincasa hispida] >XP_038898379.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898380.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898381.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898382.1 delta(24)-sterol reductase [Benincasa hispida])

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 529/567 (93.30%), Postives = 551/567 (97.18%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL  PLR KRKKVWVDYFVQFRWILV+FVVLPISFSLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLVVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVQKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+ RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADG+VVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+ GTL++IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+M+GRYASK EAKKR
Sbjct: 241 PFRGTLKEIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHA+TALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHRIFKLPVKTMIYPEPGFE  RRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHRIFKLPVKTMIYPEPGFELQRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD GLYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAGLYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEVREA  EQAH ETTYAEL+QP
Sbjct: 541 RKTEKEVREA--EQAHLETTYAELEQP 565

[Close]

BLAST of Moc09g05860 vs. NCBI nr
Match: XP_023548025.1 (delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548028.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548029.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548030.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548031.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548032.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548033.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548034.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548035.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548036.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548037.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 525/567 (92.59%), Postives = 553/567 (97.53%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFVQFRWILV+FVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D++RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADG+VVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+IGTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+M+GRYASK EAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILP ADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPLADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDAL+WAHRELEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALDWAHRELEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD  LYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETTYAEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTYAELEQP 565

[Close]

BLAST of Moc09g05860 vs. NCBI nr
Match: XP_022953594.1 (delta(24)-sterol reductase [Cucurbita moschata] >XP_022953595.1 delta(24)-sterol reductase [Cucurbita moschata] >XP_022953596.1 delta(24)-sterol reductase [Cucurbita moschata] >XP_022953597.1 delta(24)-sterol reductase [Cucurbita moschata])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 525/567 (92.59%), Postives = 552/567 (97.35%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFVQFRWILV+FVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D++RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADG++VRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+IGTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+MSGRYASK EAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD  LYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETT AEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTCAELEQP 565

[Close]

BLAST of Moc09g05860 vs. ExPASy Swiss-Prot
Match: P93472 (Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 4.4e-287
Identity = 464/567 (81.83%), Postives = 519/567 (91.53%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKK+WVDYFV+FRWILV+FVVLPISF+LYF TYLGDVRS WKS+K RQK
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENV+KVV RLK+RNP+KDGLVCTARKPW+AVGMRNVDYKRARHFEVDLS FRNIL+I
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D++RMIA+VEPLVNMGQI+R TVP+NL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVA+EI+LADG +V+ATKDN+YSDLFYAIPWSQGT+GLLVAAE+KLIPIKEYM++TYK
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P +G L+DI QAY DS APRDGDQDN EKVPDFVETM+Y+P+ AV M+GRYASK EAKK+
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GN INN+GWW+K WFYQHA TALK+G FVEYIPTREYYHRHTR LYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF  GWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKV DALEW+ RE+E+YPI
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPH++FKLP+KTMIYPE GFE  RRQGDT  AQM+TDVG+YYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +E W++ENH FQ  YAVSELNEK+FWRMFD GLYE CR+KYGAVGTFM+VYYK KKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEVREA  EQAH +T Y E+DQP
Sbjct: 541 RKTEKEVREA--EQAHLDTAYPEVDQP 565

[Close]

BLAST of Moc09g05860 vs. ExPASy Swiss-Prot
Match: Q39085 (Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2)

HSP 1 Score: 930.6 bits (2404), Expect = 8.3e-270
Identity = 444/566 (78.45%), Postives = 501/566 (88.52%), Query Frame = 0


Query: 1   MSDLQTPL-RRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDLQTPL R KRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KQHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILE 120
           K+HDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTY 240
           +DTV AYEIVLA G +VRAT+DN+YSDL+YAIPWSQGT+GLLVAAEI+LI +KEYMR+TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKK 300
            P  G L+ + Q Y+DS AP+DGD+    K+PDFVE MVY P+E V+M G YASK EAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           +GN INN+GWWFKPWFYQHA TALK+G+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+ +GWLMPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSE 480
           IWLCPH++FK P+K  IYPEPGFE+  RQGDT  AQMYTDVG+YYAPG VLRGE FDGSE
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKK 540
           AVR +EKWL+ENHGFQ  YAVSEL+EK FWRMF+G LYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAELEQAHHETTYAELD 566
           GRKTEKEVREA  EQAH ET YAE D
Sbjct: 541 GRKTEKEVREA--EQAHLETAYAEAD 561

[Close]

BLAST of Moc09g05860 vs. ExPASy Swiss-Prot
Match: Q60HC5 (Delta(24)-sterol reductase OS=Macaca fascicularis OX=9541 GN=DHCR24 PE=2 SV=2)

HSP 1 Score: 382.9 bits (982), Expect = 6.4e-105
Identity = 216/525 (41.14%), Postives = 309/525 (58.86%), Query Frame = 0


Query: 24  RWILVVFVVLPIS--FSLYFFTYLGDVRSAWKSYK--KRQKQHDENVKKVVKRLKQ-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + H++ V+ + K++++ +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEIDQQRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +ILE+D ++ I +VEPLV MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQV 150

Query: 144 SRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR 
Sbjct: 151 TALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRC 210

Query: 204 TKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYKPYIGTLRDIGQAYMDSLA 263
           T  ++ SDLFYA+PWS GT+G LVAAEI++IP K+Y+++ ++P  G      +A      
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVQGL-----EAICAKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKRGNVINNIGWWFKPWFYQH 323
                Q+N      FVE ++Y+  EAVIM+G    +VE  K    +N+IG ++KPWF++H
Sbjct: 271 HESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEVEPSK----LNSIGNYYKPWFFKH 330

Query: 324 AATALKRG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFFMGWLMPPKVSLLK 383
               LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+  GW++PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRIFKLPVKTMIY 443
            TQGE +R  Y + HV+QDMLVP+  +  AL     ++ VYPIWLCP   F LP +  + 
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILPSQPGLV 450

Query: 444 PEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEAVRGLEKWLLENHGFQAL 503
              G E          A++Y D+G Y  P    R + F+    +R LEK++   HGFQ L
Sbjct: 451 HPKGNE----------AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFDG LY + R+K G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

[Close]

BLAST of Moc09g05860 vs. ExPASy Swiss-Prot
Match: Q15392 (Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 381.3 bits (978), Expect = 1.9e-104
Identity = 215/525 (40.95%), Postives = 308/525 (58.67%), Query Frame = 0


Query: 24  RWILVVFVVLPIS--FSLYFFTYLGDVRSAWKSYK--KRQKQHDENVKKVVKRLKQ-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + H++ V+ + K++++ +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEIDQQRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +ILE+D ++ I +VEPLV MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQV 150

Query: 144 SRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR 
Sbjct: 151 TALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRC 210

Query: 204 TKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYKPYIGTLRDIGQAYMDSLA 263
           T  ++ SDLFYA+PWS GT+G LVAAEI++IP K+Y+++ ++P  G      +A      
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICAKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKRGNVINNIGWWFKPWFYQH 323
                Q+N      FVE ++Y+  EAVIM+G    + E  K    +N+IG ++KPWF++H
Sbjct: 271 HESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNYYKPWFFKH 330

Query: 324 AATALKRG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFFMGWLMPPKVSLLK 383
               LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+  GW++PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRIFKLPVKTMIY 443
            TQGE +R  Y + HV+QDMLVP+  +  AL     ++ VYPIWLCP   F LP +  + 
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILPSQPGLV 450

Query: 444 PEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEAVRGLEKWLLENHGFQAL 503
              G E          A++Y D+G Y  P    R + F+    +R LEK++   HGFQ L
Sbjct: 451 HPKGNE----------AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFDG LY + R+K G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

[Close]

BLAST of Moc09g05860 vs. ExPASy Swiss-Prot
Match: Q8VCH6 (Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.4e-104
Identity = 216/525 (41.14%), Postives = 311/525 (59.24%), Query Frame = 0


Query: 24  RWILVVFVVLPIS--FSLYFFTYLGDVRSAWKSYK--KRQKQHDENVKKVVKRLKQ-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + H++ V+ + K++++ +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEIDQQRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +ILE+D ++ I +VEPLV+MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVSMGQV 150

Query: 144 SRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++LADG  VR 
Sbjct: 151 TALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELILADGSFVRC 210

Query: 204 TKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYKPYIGTLRDIGQAYMDSLA 263
           T  ++ SDLFYA+PWS GT+G LVAAEI++IP K+Y+++ ++P  G      +A  +   
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICEKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKRGNVINNIGWWFKPWFYQH 323
                 +N      FVE ++Y+  EAVIM+G     VE  K    +N+IG ++KPWF++H
Sbjct: 271 RESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSYYKPWFFKH 330

Query: 324 AATALKRG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFFMGWLMPPKVSLLK 383
               LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+  GW++PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRIFKLPVKTMIY 443
            TQGE +R  Y + HV+QDMLVP+  +  AL     ++ VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEAVRGLEKWLLENHGFQAL 503
            +PG      +GD   A++Y D+G Y  P    R + F+    +R LEK++   HGFQ L
Sbjct: 451 SQPGLV--HPKGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFDG LY + RK+ G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

[Close]

BLAST of Moc09g05860 vs. ExPASy TrEMBL
Match: A0A6J1DEM5 (Delta(24)-sterol reductase OS=Momordica charantia OX=3673 GN=LOC111019723 PE=4 SV=1)

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI
Sbjct: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR
Sbjct: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI
Sbjct: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA
Sbjct: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQPQ 569
           RKTEKEVREAELEQAHHETTYAELDQPQ
Sbjct: 541 RKTEKEVREAELEQAHHETTYAELDQPQ 568

[Close]

BLAST of Moc09g05860 vs. ExPASy TrEMBL
Match: A0A6J1GQ50 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 525/567 (92.59%), Postives = 552/567 (97.35%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFVQFRWILV+FVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D++RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLADG++VRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+IGTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+MSGRYASK EAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD  LYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETT AEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTCAELEQP 565

[Close]

BLAST of Moc09g05860 vs. ExPASy TrEMBL
Match: A0A6J1JUW6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1)

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 524/567 (92.42%), Postives = 552/567 (97.35%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFVQFRWILV+FVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D++RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYEIVLA+G+VVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEIVLANGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+IGTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+M GRYASK EAK +
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMKGRYASKEEAKIK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+GEFVEY+PTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYVPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD  LYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETTYAEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTYAELEQP 565

[Close]

BLAST of Moc09g05860 vs. ExPASy TrEMBL
Match: A0A6J1H6V4 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 522/567 (92.06%), Postives = 552/567 (97.35%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFV+FRWILV+FVVLPISF LYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQ++MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYE+VLADG+VVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+ GTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+M+GRYASKVEAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHA+TALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF  GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD   YEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETTYAEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTYAELEQP 565

[Close]

BLAST of Moc09g05860 vs. ExPASy TrEMBL
Match: A0A6J1KZQ6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 521/567 (91.89%), Postives = 552/567 (97.35%), Query Frame = 0


Query: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL+ PLR KRKKVWVDYFV+FRWILV+FVVLPISF LYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120
           +HDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DQ++MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240
           DTVVAYE+VLADG+VVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300
           P+ GTLR+IGQAYMDSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+M+GRYASKVEAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHA+TALK+GEFVEYIPTR+YYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF  GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDTHYAQMYTDVGLYYAPGP+LRGE+FDG+EA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEIFDGAEA 480

Query: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD   YEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAELEQAHHETTYAELDQP 568
           RKTEKEV+EA  EQAH ETTYAEL+QP
Sbjct: 541 RKTEKEVKEA--EQAHLETTYAELEQP 565

[Close]

BLAST of Moc09g05860 vs. TAIR 10
Match: AT3G19820.1 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 930.6 bits (2404), Expect = 5.9e-271
Identity = 444/566 (78.45%), Postives = 501/566 (88.52%), Query Frame = 0


Query: 1   MSDLQTPL-RRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDLQTPL R KRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KQHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILE 120
           K+HDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTY 240
           +DTV AYEIVLA G +VRAT+DN+YSDL+YAIPWSQGT+GLLVAAEI+LI +KEYMR+TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKK 300
            P  G L+ + Q Y+DS AP+DGD+    K+PDFVE MVY P+E V+M G YASK EAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           +GN INN+GWWFKPWFYQHA TALK+G+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+ +GWLMPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSE 480
           IWLCPH++FK P+K  IYPEPGFE+  RQGDT  AQMYTDVG+YYAPG VLRGE FDGSE
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKK 540
           AVR +EKWL+ENHGFQ  YAVSEL+EK FWRMF+G LYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAELEQAHHETTYAELD 566
           GRKTEKEVREA  EQAH ET YAE D
Sbjct: 541 GRKTEKEVREA--EQAHLETAYAEAD 561

[Close]

BLAST of Moc09g05860 vs. TAIR 10
Match: AT3G19820.2 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 930.6 bits (2404), Expect = 5.9e-271
Identity = 444/566 (78.45%), Postives = 501/566 (88.52%), Query Frame = 0


Query: 1   MSDLQTPL-RRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDLQTPL R KRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KQHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILE 120
           K+HDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTY 240
           +DTV AYEIVLA G +VRAT+DN+YSDL+YAIPWSQGT+GLLVAAEI+LI +KEYMR+TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKK 300
            P  G L+ + Q Y+DS AP+DGD+    K+PDFVE MVY P+E V+M G YASK EAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           +GN INN+GWWFKPWFYQHA TALK+G+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+ +GWLMPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSE 480
           IWLCPH++FK P+K  IYPEPGFE+  RQGDT  AQMYTDVG+YYAPG VLRGE FDGSE
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKK 540
           AVR +EKWL+ENHGFQ  YAVSEL+EK FWRMF+G LYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAELEQAHHETTYAELD 566
           GRKTEKEVREA  EQAH ET YAE D
Sbjct: 541 GRKTEKEVREA--EQAHLETAYAEAD 561

[Close]

BLAST of Moc09g05860 vs. TAIR 10
Match: AT3G19820.3 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 930.6 bits (2404), Expect = 5.9e-271
Identity = 444/566 (78.45%), Postives = 501/566 (88.52%), Query Frame = 0


Query: 1   MSDLQTPL-RRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDLQTPL R KRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KQHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILE 120
           K+HDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I++++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTY 240
           +DTV AYEIVLA G +VRAT+DN+YSDL+YAIPWSQGT+GLLVAAEI+LI +KEYMR+TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKK 300
            P  G L+ + Q Y+DS AP+DGD+    K+PDFVE MVY P+E V+M G YASK EAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           +GN INN+GWWFKPWFYQHA TALK+G+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+ +GWLMPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSE 480
           IWLCPH++FK P+K  IYPEPGFE+  RQGDT  AQMYTDVG+YYAPG VLRGE FDGSE
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKK 540
           AVR +EKWL+ENHGFQ  YAVSEL+EK FWRMF+G LYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAELEQAHHETTYAELD 566
           GRKTEKEVREA  EQAH ET YAE D
Sbjct: 541 GRKTEKEVREA--EQAHLETAYAEAD 561

[Close]

BLAST of Moc09g05860 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 53.1 bits (126), Expect = 8.3e-07
Identity = 44/144 (30.56%), Postives = 67/144 (46.53%), Query Frame = 0


Query: 138 ISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVVR 197
           I R T+   LS     +   LTVGG ++  GI G +  +G   + V   EIV   G VV 
Sbjct: 170 ILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVT 229

Query: 198 ATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIP---IKEYMRVTYKPYIGTLRDIGQAYM 257
            + + + S+LF+++    G  G++  A I L P   + +++RV Y  +    RD  Q Y+
Sbjct: 230 CS-EKRNSELFFSVLGGLGQFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRD--QEYL 289

Query: 258 DSLAPRDGDQDNPEKVPDFVETMV 279
            S           EK  D+VE  V
Sbjct: 290 IS----------KEKTFDYVEGFV 300

[Close]

BLAST of Moc09g05860 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 48.9 bits (115), Expect = 1.6e-05
Identity = 28/92 (30.43%), Postives = 46/92 (50.00%), Query Frame = 0


Query: 137 QISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVV 196
           ++   T+ L L+     +   LTVGG ++  GI G +  YG     V+  +++   G + 
Sbjct: 150 EVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIA 209

Query: 197 RATKDNKYSDLFYAIPWSQGTIGLLVAAEIKL 229
             +KD   SDLF+A+    G  G++  A IKL
Sbjct: 210 TCSKDMN-SDLFFAVLGGLGQFGIITRARIKL 240

The following BLAST results are available for this feature:

BLAST of Moc09g05860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151841.1	0.0e+00	100.00	delta(24)-sterol reductase-like [Momordica charantia] >XP_022151850.1 delta(24)-...	[more]
KAG7014278.1	0.0e+00	92.77	Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038898378.1	0.0e+00	93.30	delta(24)-sterol reductase [Benincasa hispida] >XP_038898379.1 delta(24)-sterol ...	[more]
XP_023548025.1	0.0e+00	92.59	delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24...	[more]
XP_022953594.1	0.0e+00	92.59	delta(24)-sterol reductase [Cucurbita moschata] >XP_022953595.1 delta(24)-sterol...	[more]

BLAST of Moc09g05860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93472	4.4e-287	81.83	Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1	[more]
Q39085	8.3e-270	78.45	Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2	[more]
Q60HC5	6.4e-105	41.14	Delta(24)-sterol reductase OS=Macaca fascicularis OX=9541 GN=DHCR24 PE=2 SV=2	[more]
Q15392	1.9e-104	40.95	Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2	[more]
Q8VCH6	2.4e-104	41.14	Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1	[more]

BLAST of Moc09g05860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DEM5	0.0e+00	100.00	Delta(24)-sterol reductase OS=Momordica charantia OX=3673 GN=LOC111019723 PE=4 S...	[more]
A0A6J1GQ50	0.0e+00	92.59	Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV...	[more]
A0A6J1JUW6	0.0e+00	92.42	Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1	[more]
A0A6J1H6V4	0.0e+00	92.06	Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV...	[more]
A0A6J1KZQ6	0.0e+00	91.89	Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1	[more]

BLAST of Moc09g05860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19820.1	5.9e-271	78.45	cell elongation protein / DWARF1 / DIMINUTO (DIM)	[more]
AT3G19820.2	5.9e-271	78.45	cell elongation protein / DWARF1 / DIMINUTO (DIM)	[more]
AT3G19820.3	5.9e-271	78.45	cell elongation protein / DWARF1 / DIMINUTO (DIM)	[more]
AT2G41510.1	8.3e-07	30.56	cytokinin oxidase/dehydrogenase 1	[more]
AT5G56970.1	1.6e-05	30.43	cytokinin oxidase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 109..198 e-value: 8.0E-15 score: 54.8
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 110..231 e-value: 3.3E-26 score: 93.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 540..568
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 540..560
None	No IPR available	PANTHER	PTHR10801:SF16	S-LOCUS GLYCOPROTEIN DOMAIN, BULB-TYPE LECTIN DOMAIN, FAD-BINDING, TYPE 2-RELATED	coord: 1..566
IPR040165	Delta(24)-sterol reductase	PANTHER	PTHR10801	24-DEHYDROCHOLESTEROL REDUCTASE	coord: 1..566
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 45..231 score: 12.82659
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 105..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g05860.1	Moc09g05860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008202	steroid metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0050614	delta24-sterol reductase activity
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity