Moc09g05760 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g05760
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCytochrome P450
Locationchr9: 4413679 .. 4416728 (+)
RNA-Seq ExpressionMoc09g05760
SyntenyMoc09g05760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTCCAATGCAATTCCATTCCTTCATCACAAACCTATGATGGATTCCAGCAACCTCTCCTCCTCCTCCTCCTCCCTGCCCCCTCTAATTTCCTCCGGGAAGTTCGGGTTCCGGCAGCTCCTCGATGTCCAAATCTTAGAGATCTTCTTTGCTCTTTTCGTGTTCCTGGCCATCCATGTCGTTAGACAGAAGCGACGTTACGGCTTGCCAGTGTGGCCAGTTGTTGGGATGTTACCAGCTCTGATCACGGCGGCGCGTGGGAACCTATACGAATGGATAACTGAACTCCTCCGCCGCCACAAGGGGACATTTCAATTTCACGGCCCGTGGCTGAGCAGCCTCAACTGCGTCGTCACCGCCGATCCACGGAACTTGGAGCATCTTCTCAAGACCAAATTTCCCTCCTACCCTAAAGGGCCGTTCTTCCGCGACACGGTGAGGGACCTCCTCGGGGACGGGATATTCGGCGCCGACGATGAGACGTGGCAGAGACAGAGGAAGACGGCGAGTATAGAGTTTCACTCGGCAAAGTTCCGGCGGATGACGGTGGAGTCGCTGGTGGAGCTGGTGCACCGGAGGCTTGAAGGGGTGTTGGAGGATTACGCCGGACGGCCGGCGGCTGTGGACCTGCAGGATGTGCTTCTGAGGCTGACGTTCGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGCTGCTTGCGCGGCGGCTTGCCGGAGATTCCATTTGCTAAGGCTTTTGAGGACGCTACAGAGGCCACTGTGTTGCGCTTCATCACACCTACATTCATATGGAAAGCCATGAGGTATAATCTCTAGTTTGAAAATTTGCAAATAGTTTTCTGAGAGTATCTTTTTAATCTTATGTCTATAATTTTTTTAATATTGTTTTTGCAAATAATATAAGAAATGCTTTCTATTATCTATGTTGGGCGAAGGCTTTTTGTGAAGGGTGTCCAAATGGCAGTTGTGGAACTGAAAAGTAACTTGGTTTTGTTTCGCGGTGAAATTTTGGTGGGAAGACAAGAAACTCAAAAATCGGAACTTTTTTCTTTTGATTGTTTACTTTAATCCAATGAATTCATAAGTAACAAAGGAGTTTTAGGACCAATTTGGTAAAGTTTTAGTTTTAGTTTTTTTTTTCCCTCTAATTTTTCGATGTTTAAGAAACATAAGCATTTGATAAGTATGTACATTTCTCCTTTTTATGAAACATTCCTAAAATTGTATATTAGTTTTACATTTCTAGTCTAGAAATATAAAATGCATAATAAATATTAAAAAATAAAGATAACTAAAAATTTTGAACATTTAATATAAAAAAAAATTCATGTAAAATTTTTACTTCTCTCTTTTTATTTATTTATTAACAATTTTAAAGAAAGAAATACAAGTTGAAAGGGTTACTATTTGTCAAATGTAATTATGTCTTTATTCATTAAAAAAGAGACTTGCTAGTTAGAAAGGCTTTTTTTAAGTGTAACAAATGTTTGGGAAAGAATCTAACCTTCAATCTTGATAAAGGTAATAAATATCTTATAACTAAACTATCTCTCATTGACCACCTTGGTAGTAAGACTTACTTGTACAATCAACGGGCATTGGTTGTTTTGTACCAACTTTTGTATAAGACTTTTATTTTCCACTAAAATATCAATCTCACCCTCGCATATTGTTTGTTAAGAAAAAAAATGAGACCTACTTATGCTCATCATAAGTAGGTAAAATGGCAAATATTTAACAAAGAAATTTGTTATTTTAACCTATTTTGGTCTCCTTTTCGACATAGTACCTTAAAAAAATAACAAGTTTGTTTTCAAATTTACATTATTTTTTTTTTAAGAATAGTCTAGACGATGGTAAAGAAGAACTTTGATATCAGTAGGAGCTTCTAAGTCGTCTTCTTTTTTGTAACGCAGAAATTATCAAGTTTGGAAAATAATATGGTTTAATGCGCTAAAAAATACTACCAAAAAACAAAATAAAAAAACAAACATTTTATTAAGTAAAACTCTTTAAAATCTTTAATTAAAATTAAGTGATATATTAAAACAAATTAAATTAAGTGGGTAATTAATTTACTTTTTGAGCAGGTACCTGAACTTGGGAATGGAGAAGAAGCTGAAGCTATCGATAAAGGCCGTCGACGAATTTGCAGAGAACGTGATTCGAACACGGAAGAGAGAACTCTCCGCGGCGGCGCAACCGCAAGCCGAGAATCAAAGATCCGATCTATTGACCGTGTTCATGAGGCTGCGGGACGAGCAAGGGCGGCCATTCTCCGACAAGTTCCTCAGAGATATCTGCGTCAATTTCATACTCGCAGGACGAGACACATCTTCTGTCGCATTAACCTGGTTTTTCTGGTTGCTCGATCAAAATCCTGATGTGGAAGAGAAAACTCTCGCAGAGATTTGCCGAATCGTCGGCGAACGAGAAGAGGCCCAAACCGGAGAGCTTCGATTCGATTCGTTGATGTTCAAGCCAGAGGAAATCAAGAAAATGGAGTATTTGCACGCCGCAATTTCCGAAGCCCTAAGATTGTATCCTTCAGTACCCGTCGATCATAAAGAGGTAATTAATCATTCATATTCCTCTTCTGAAAACGAATTTCAGAGATCTGATTAATAATCCTTCCGATTCAGGTCGTGGAGGACGATGTATTCCCCGACGGAACGATTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCGATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGCCGGTTCATGAGCGAATCGGCGTATCGATTCGCCGCCTTCAACGGCGGACCTCGGCTGTGTCTAGGAAAGGATTTCGCGTACTACCAAATGAAGTACGTCGCCGCTTCGATCATCCGCCGGTACCGGGTGACGGTGGCGGCGGGCCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGCACGGGCTGAAGGTCAATTTGATTAAGCGCGAAGAGGGGCAGATTCGGAAATTCATGAAGTTAAATTGA

mRNA sequence

ATGTTTTCCAATGCAATTCCATTCCTTCATCACAAACCTATGATGGATTCCAGCAACCTCTCCTCCTCCTCCTCCTCCCTGCCCCCTCTAATTTCCTCCGGGAAGTTCGGGTTCCGGCAGCTCCTCGATGTCCAAATCTTAGAGATCTTCTTTGCTCTTTTCGTGTTCCTGGCCATCCATGTCGTTAGACAGAAGCGACGTTACGGCTTGCCAGTGTGGCCAGTTGTTGGGATGTTACCAGCTCTGATCACGGCGGCGCGTGGGAACCTATACGAATGGATAACTGAACTCCTCCGCCGCCACAAGGGGACATTTCAATTTCACGGCCCGTGGCTGAGCAGCCTCAACTGCGTCGTCACCGCCGATCCACGGAACTTGGAGCATCTTCTCAAGACCAAATTTCCCTCCTACCCTAAAGGGCCGTTCTTCCGCGACACGGTGAGGGACCTCCTCGGGGACGGGATATTCGGCGCCGACGATGAGACGTGGCAGAGACAGAGGAAGACGGCGAGTATAGAGTTTCACTCGGCAAAGTTCCGGCGGATGACGGTGGAGTCGCTGGTGGAGCTGGTGCACCGGAGGCTTGAAGGGGTGTTGGAGGATTACGCCGGACGGCCGGCGGCTGTGGACCTGCAGGATGTGCTTCTGAGGCTGACGTTCGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGCTGCTTGCGCGGCGGCTTGCCGGAGATTCCATTTGCTAAGGCTTTTGAGGACGCTACAGAGGCCACTGTGTTGCGCTTCATCACACCTACATTCATATGGAAAGCCATGAGGTACCTGAACTTGGGAATGGAGAAGAAGCTGAAGCTATCGATAAAGGCCGTCGACGAATTTGCAGAGAACGTGATTCGAACACGGAAGAGAGAACTCTCCGCGGCGGCGCAACCGCAAGCCGAGAATCAAAGATCCGATCTATTGACCGTGTTCATGAGGCTGCGGGACGAGCAAGGGCGGCCATTCTCCGACAAGTTCCTCAGAGATATCTGCGTCAATTTCATACTCGCAGGACGAGACACATCTTCTGTCGCATTAACCTGGTTTTTCTGGTTGCTCGATCAAAATCCTGATGTGGAAGAGAAAACTCTCGCAGAGATTTGCCGAATCGTCGGCGAACGAGAAGAGGCCCAAACCGGAGAGCTTCGATTCGATTCGTTGATGTTCAAGCCAGAGGAAATCAAGAAAATGGAGTATTTGCACGCCGCAATTTCCGAAGCCCTAAGATTGTATCCTTCAGTACCCGTCGATCATAAAGAGGTCGTGGAGGACGATGTATTCCCCGACGGAACGATTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCGATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGCCGGTTCATGAGCGAATCGGCGTATCGATTCGCCGCCTTCAACGGCGGACCTCGGCTGTGTCTAGGAAAGGATTTCGCGTACTACCAAATGAAGTACGTCGCCGCTTCGATCATCCGCCGGTACCGGGTGACGGTGGCGGCGGGCCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGCACGGGCTGAAGGTCAATTTGATTAAGCGCGAAGAGGGGCAGATTCGGAAATTCATGAAGTTAAATTGA

Coding sequence (CDS)

ATGTTTTCCAATGCAATTCCATTCCTTCATCACAAACCTATGATGGATTCCAGCAACCTCTCCTCCTCCTCCTCCTCCCTGCCCCCTCTAATTTCCTCCGGGAAGTTCGGGTTCCGGCAGCTCCTCGATGTCCAAATCTTAGAGATCTTCTTTGCTCTTTTCGTGTTCCTGGCCATCCATGTCGTTAGACAGAAGCGACGTTACGGCTTGCCAGTGTGGCCAGTTGTTGGGATGTTACCAGCTCTGATCACGGCGGCGCGTGGGAACCTATACGAATGGATAACTGAACTCCTCCGCCGCCACAAGGGGACATTTCAATTTCACGGCCCGTGGCTGAGCAGCCTCAACTGCGTCGTCACCGCCGATCCACGGAACTTGGAGCATCTTCTCAAGACCAAATTTCCCTCCTACCCTAAAGGGCCGTTCTTCCGCGACACGGTGAGGGACCTCCTCGGGGACGGGATATTCGGCGCCGACGATGAGACGTGGCAGAGACAGAGGAAGACGGCGAGTATAGAGTTTCACTCGGCAAAGTTCCGGCGGATGACGGTGGAGTCGCTGGTGGAGCTGGTGCACCGGAGGCTTGAAGGGGTGTTGGAGGATTACGCCGGACGGCCGGCGGCTGTGGACCTGCAGGATGTGCTTCTGAGGCTGACGTTCGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGCTGCTTGCGCGGCGGCTTGCCGGAGATTCCATTTGCTAAGGCTTTTGAGGACGCTACAGAGGCCACTGTGTTGCGCTTCATCACACCTACATTCATATGGAAAGCCATGAGGTACCTGAACTTGGGAATGGAGAAGAAGCTGAAGCTATCGATAAAGGCCGTCGACGAATTTGCAGAGAACGTGATTCGAACACGGAAGAGAGAACTCTCCGCGGCGGCGCAACCGCAAGCCGAGAATCAAAGATCCGATCTATTGACCGTGTTCATGAGGCTGCGGGACGAGCAAGGGCGGCCATTCTCCGACAAGTTCCTCAGAGATATCTGCGTCAATTTCATACTCGCAGGACGAGACACATCTTCTGTCGCATTAACCTGGTTTTTCTGGTTGCTCGATCAAAATCCTGATGTGGAAGAGAAAACTCTCGCAGAGATTTGCCGAATCGTCGGCGAACGAGAAGAGGCCCAAACCGGAGAGCTTCGATTCGATTCGTTGATGTTCAAGCCAGAGGAAATCAAGAAAATGGAGTATTTGCACGCCGCAATTTCCGAAGCCCTAAGATTGTATCCTTCAGTACCCGTCGATCATAAAGAGGTCGTGGAGGACGATGTATTCCCCGACGGAACGATTCTGAAGAAAGGAACGAAAATCATCTACGCAATATACGCGATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGCCGGTTCATGAGCGAATCGGCGTATCGATTCGCCGCCTTCAACGGCGGACCTCGGCTGTGTCTAGGAAAGGATTTCGCGTACTACCAAATGAAGTACGTCGCCGCTTCGATCATCCGCCGGTACCGGGTGACGGTGGCGGCGGGCCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGCACGGGCTGAAGGTCAATTTGATTAAGCGCGAAGAGGGGCAGATTCGGAAATTCATGAAGTTAAATTGA

Protein sequence

MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIHVVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKREEGQIRKFMKLN
Homology
BLAST of Moc09g05760 vs. NCBI nr
Match: XP_022153724.1 (cytochrome P450 86B1 [Momordica charantia])

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH
Sbjct: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT
Sbjct: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR
Sbjct: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR
Sbjct: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF
Sbjct: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           KHGLKVNLIKREEGQIRKFMKLN
Sbjct: 541 KHGLKVNLIKREEGQIRKFMKLN 563

BLAST of Moc09g05760 vs. NCBI nr
Match: KAG7014270.1 (Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1023.8 bits (2646), Expect = 5.4e-295
Identity = 502/563 (89.17%), Postives = 530/563 (94.14%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFS+AIPFLHHKPMMDS NLS+SS+  P L+S+G FG   LLD QILEI FALF+FLAIH
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSTSSAP-PALVSAGNFGLWPLLDFQILEISFALFMFLAIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           VVRQ+RRYGLPVWP+VGMLP+LITAARGNLYEWIT+LLRRHKGTFQF GPW SSLNCVVT
Sbjct: 61  VVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+FR
Sbjct: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           +MTVESLVELVH RLE VLED+ GR  AVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 QMTVESLVELVHSRLETVLEDFVGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATE TVLRFITPTF+WK +RYLNLG+EKKLK SI+AVDEFAE+VI+TRKR
Sbjct: 241 EIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           ELS  AQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL+WFF
Sbjct: 301 ELSTTAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLD+NPDVE+K LAEI RIVGEREEA+T E RFDSL+FKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+DGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRF AFNGGPRLCLG+DFAYYQMKYVAASII RYRV V  GHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           K GLKVNLIKREEGQIRK++KLN
Sbjct: 541 KDGLKVNLIKREEGQIRKYVKLN 562

BLAST of Moc09g05760 vs. NCBI nr
Match: XP_023549182.1 (cytochrome P450 86B1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023.5 bits (2645), Expect = 7.1e-295
Identity = 504/565 (89.20%), Postives = 528/565 (93.45%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSS--LPPLISSGKFGFRQLLDVQILEIFFALFVFLA 60
           MFS+AIPFLHHKPMMDS NLSSSSSS  LP L+S GKFG   LLDVQILEI FALFVF A
Sbjct: 1   MFSHAIPFLHHKPMMDSGNLSSSSSSSALPALVSDGKFGLWPLLDVQILEISFALFVFFA 60

Query: 61  IHVVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCV 120
           IHVVRQ+RRYGLPVWP+VGMLP+LITAARGNLYEWIT+LLRRHKGTFQF GPW SSLNCV
Sbjct: 61  IHVVRQRRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCV 120

Query: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAK 180
           VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 181 FRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240
           FR+MTVESLVELVH RLE VLED+ GR  AVDLQDVLLRLTFDNVCMIAFGVDPGCL GG
Sbjct: 181 FRQMTVESLVELVHLRLETVLEDFVGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLSGG 240

Query: 241 LPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTR 300
           LPEIPFAKAFEDATE TVLRFITPTF+WK +RYLNLG+EK+LK SI+AVDEFAENVI+TR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKRLKQSIQAVDEFAENVIQTR 300

Query: 301 KRELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTW 360
           KRELS  AQPQA+NQRSDLLTVFMRLRDEQGR FSDKFLRDICVNFILAGRDTSSVAL+W
Sbjct: 301 KRELSTTAQPQADNQRSDLLTVFMRLRDEQGRSFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 361 FFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEAL 420
           FFWLLD+NPDVE+K LAEI RIVGEREEA+T E RFDSL+FKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 480
           RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+D
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 481 GRFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTF 540
           GRFMSESAYRF AFNGGPRLCLG+DFAYYQMKYVAASII RYRV V  GHPVEPKLALTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 541 YMKHGLKVNLIKREEGQIRKFMKLN 564
           YMK GLKVNLIKR EGQIRK+ KLN
Sbjct: 541 YMKDGLKVNLIKRNEGQIRKYTKLN 565

BLAST of Moc09g05760 vs. NCBI nr
Match: XP_022954102.1 (cytochrome P450 86B1-like [Cucurbita moschata])

HSP 1 Score: 1022.7 bits (2643), Expect = 1.2e-294
Identity = 503/563 (89.34%), Postives = 529/563 (93.96%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFS+AIPFLHHKPMMDS NL SSSS+LP L+S+G FG   LLD QILEI FALFVFLAIH
Sbjct: 1   MFSHAIPFLHHKPMMDSGNL-SSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           VVRQ+RRYGLPVWP+VGMLP+LITAARGNLYEWIT+LLR HKGTFQF GPW SSLNCVVT
Sbjct: 61  VVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+FR
Sbjct: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           +MTVESLVELVH RLE VLED+ GR  AVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 QMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATE TVLRFITPTF+WK +RYLNLG+EKKLK SI+AVDEFAE+VI+TRKR
Sbjct: 241 EIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           ELS  AQPQAENQRSDLLTVFMRLRDEQ RPFSDKFLRDICVNFILAGRDTSSVAL+WFF
Sbjct: 301 ELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLD+NPDVE+K LAEI RIVGEREEA+T E RFDSL+FKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+DGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRF AFNGGPRLCLG+DFAYYQMKYVAASII RYRV V  GHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           K GLKVNLIKREEGQIRK++KLN
Sbjct: 541 KDGLKVNLIKREEGQIRKYVKLN 562

BLAST of Moc09g05760 vs. NCBI nr
Match: XP_038899430.1 (cytochrome P450 86B1-like [Benincasa hispida])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 503/563 (89.34%), Postives = 520/563 (92.36%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFS+A PFLHHKPMMDS NLSSSSS LPP IS   F  R LLDVQILEI FALFVFL IH
Sbjct: 1   MFSHACPFLHHKPMMDSGNLSSSSSPLPPPISVANFRLRPLLDVQILEISFALFVFLVIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           VVRQKRRY LPVWP+VGMLPALITAARGN+YEWITELL RHKGTFQF GPWLSSL+CVVT
Sbjct: 61  VVRQKRRYDLPVWPLVGMLPALITAARGNIYEWITELLHRHKGTFQFRGPWLSSLHCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+FR
Sbjct: 121 ADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           +MTV+SLVELVH RLE VLE++AGR   VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 QMTVDSLVELVHSRLEPVLENFAGRSEVVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATE TVLRFITPTFIWK MRYLNLGMEKKLK SIKAVDEFAENVI+TRKR
Sbjct: 241 EIPFAKAFEDATEVTVLRFITPTFIWKVMRYLNLGMEKKLKQSIKAVDEFAENVIQTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           E   AAQPQA++QRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL+WFF
Sbjct: 301 EFYTAAQPQADDQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLDQNPDVE K L EICRIV EREEA+T ELR DSL+FKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDQNPDVEHKILEEICRIVSEREEAKTRELRLDSLVFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDC EFKPERWMRDGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCCEFKPERWMRDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRF AFNGGPRLCLGKDFAYYQMKYVAAS I RYRV V  GHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASTISRYRVAVVTGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           K+GLKVNLIKREEGQI K+MKLN
Sbjct: 541 KNGLKVNLIKREEGQIWKYMKLN 563

BLAST of Moc09g05760 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 7.8e-220
Identity = 374/538 (69.52%), Postives = 435/538 (80.86%), Query Frame = 0

Query: 22  SSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIHVVRQKRRYGLPVWPVVGMLPA 81
           SSSSS  PL+S   F  R   DVQILE+  A+FVF+AIH +RQK+  GLPVWP +GMLP+
Sbjct: 17  SSSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 82  LITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVTADPRNLEHLLKTKFPSYPKGP 141
           L    RGN+YEW++++L    GTFQF GPW SSLN  +T DPRN+EHLLK +F  +PKG 
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 142 FFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLED 201
           +FRD +RDLLGDGIF ADDETWQRQRKTASIEFHSAKFR++T +SL ELVH+RL  VLE 
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLET 196

Query: 202 YAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFIT 261
                + +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATEA V+RF+ 
Sbjct: 197 SVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVM 256

Query: 262 PTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVF 321
           PT +WK MRYL++G EKKLK SIK VD+FA+ VIRTRK+ELS   +     +RSDLLTVF
Sbjct: 257 PTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGE---TTKRSDLLTVF 316

Query: 322 MRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIV 381
           M LRDE+G  FSDKFLRDICVNFILAGRDTSSVAL+WFFWLL++NP+VEEK + E+C+I+
Sbjct: 317 MGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKIL 376

Query: 382 GEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGT 441
            +R++    E      +F PEEIKKM+YL AA+SEALRLYPSVPVDHKEV EDDVFPDGT
Sbjct: 377 RQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGT 436

Query: 442 ILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFNGGPRLCLG 501
           +LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+RDGRFMSESAY+F AFNGGPRLCLG
Sbjct: 437 MLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLG 496

Query: 502 KDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKREEGQIRKF 560
           KDFAYYQMK  AA+I+ RY+V V  GH VEPKLALT YMKHGL VNLI R   +I ++
Sbjct: 497 KDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of Moc09g05760 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 4.2e-133
Identity = 251/517 (48.55%), Postives = 339/517 (65.57%), Query Frame = 0

Query: 42  LDVQILEIFFALFVFLAIHVVRQKRRY-GLPVWPVVGMLPALITAARGNLYEWITELLRR 101
           +D     I   L V   I  V  +R Y G  VWP+VG LPALIT A   ++++I + LR 
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 102 HKGTFQ---FHGPWLSSL--NCVVTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGI 161
             GT+Q   F  P+L+    +  VT DP+NLEH+LKT+F +YPKGP ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 162 FGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVL 221
           F +D +TW+ QRKTA++EF +   R+     +   +  RL  +LE    R   +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 222 LRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLG 281
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATEAT+ RFI P FIWK  ++L LG
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 282 MEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDK 341
           +E  +  SI  VD +   +I TRK EL    Q Q E++  DLL+ FM+ ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLEL--LGQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 342 FLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFD 401
           +L+ + +NFILAGRDTSSVA++WFFWL+  NP VEEK + EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 402 SLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYA 461
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 462 MGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAAS 521
           +GRM+ IWG+DC EFKPERW+ + R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 522 IIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKRE 553
           I+ R+R+TVA GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

BLAST of Moc09g05760 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.1e-128
Identity = 240/492 (48.78%), Postives = 320/492 (65.04%), Query Frame = 0

Query: 69  GLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQ---FHGPWLSSLN--CVVTADP 128
           G  VWP++G LP LI   R  ++EWITE LR   GT+Q   F  P+L+       VT DP
Sbjct: 29  GPRVWPLLGSLPGLI-EQRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCDP 88

Query: 129 RNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMT 188
           +NLEH+LKT+F +YPKGP ++    DLLG GIF +D +TW  QRKTA++EF +   R+  
Sbjct: 89  KNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 189 VESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 248
              +   +  R   +L         VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 249 FAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELS 308
           FA AF+ ATEA++ RFI P F+WK  ++L LG+E  L  S+  +DE+   VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEIDEYLAAVINTRKQELM 268

Query: 309 AAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLL 368
           +  +     +  DLL+ FM  + E    +SD FL+ + +NFILAGRDTSSVAL+WFFWL+
Sbjct: 269 SQQESGTHQRHDDLLSRFMMKKTES---YSDTFLQHVALNFILAGRDTSSVALSWFFWLI 328

Query: 369 DQNPDVEEKTLAEICRIVGE-REEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYP 428
             +P VE+K + EIC ++ E R          + L F  +EI ++ YL AAISE LRLYP
Sbjct: 329 TMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLGF--DEIDRLVYLKAAISETLRLYP 388

Query: 429 SVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--DGR 488
           SVP D K V  DDV PDGT +  G+ + Y+IYA GRM++ WG+DC EF PERW+   DG+
Sbjct: 389 SVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFNPERWISPIDGK 448

Query: 489 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 548
           F++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+TV  GH VE K++LT +M
Sbjct: 449 FINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVVPGHKVEQKMSLTLFM 508

Query: 549 KHGLKVNLIKRE 553
           K+GL VNL KR+
Sbjct: 509 KNGLLVNLYKRD 514

BLAST of Moc09g05760 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 9.1e-128
Identity = 253/530 (47.74%), Postives = 337/530 (63.58%), Query Frame = 0

Query: 44  VQILEIFFALFVFLAIHVVRQKRRYGLPVWPVVGMLPALITAARGN-LYEWITELLRRHK 103
           + I+ I  A  ++        K   G  +WPVVG LP LI    GN ++EWI + LR   
Sbjct: 7   MMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLI--ENGNRMHEWIADNLRACS 66

Query: 104 GTFQ---FHGPWLSSLNCV-VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGA 163
           GT+Q      P+L+    V VT DP+NLEH+LK +F +YPKGP ++    DLLG+GIF +
Sbjct: 67  GTYQTCICAIPFLARKQLVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNS 126

Query: 164 DDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRL 223
           D +TW  QRKTA++EF +   R+     +   +  R   +LE    +   VDLQD+LLRL
Sbjct: 127 DGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQDLLLRL 186

Query: 224 TFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEK 283
           TFDN+C +AFG DP  L   LPE  FA +F+ ATEAT+ RFI P F+WK  + L LG+E 
Sbjct: 187 TFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEV 246

Query: 284 KLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLR 343
            L  S+K VD +  +VI TRK EL          Q  DLL+ FM+ ++     +SDKFL+
Sbjct: 247 SLNNSLKQVDNYMTDVINTRKLEL--LNHQNGGPQHDDLLSRFMKKKES----YSDKFLQ 306

Query: 344 DICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLM 403
            + +NFILAGRDTSSVAL+WFFWL+  NP VEEK L EIC I+ E     T +   + L+
Sbjct: 307 HVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLV 366

Query: 404 FKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGR 463
           F  EE+ ++ YL AA+SE LRLYPSVP D K V+ DD  PDGT +  G+ I Y+IY+ GR
Sbjct: 367 F--EEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGR 426

Query: 464 MEAIWGKDCREFKPERWM-RDG-RFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASI 523
           M+ IWG+DC EFKPERWM +DG +F  +  +RF AFN GPR+CLGKD AY QMK +AA++
Sbjct: 427 MKFIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAV 486

Query: 524 IRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKRE----EGQIRKFMKL 563
           + R+R+ VA GH VE K++LT +MK GL +N+  R+      +I KF K+
Sbjct: 487 LLRHRLAVAPGHKVEQKMSLTLFMKDGLVMNVTPRDLTPILAKIEKFGKV 526

BLAST of Moc09g05760 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.6e-127
Identity = 234/491 (47.66%), Postives = 324/491 (65.99%), Query Frame = 0

Query: 69  GLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQ---FHGPWLSSLN--CVVTADP 128
           G  VWPV+G LP LI   R  +++WITE LR   GT+Q      P+L+       VT DP
Sbjct: 29  GPRVWPVLGSLPGLI-EQRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCDP 88

Query: 129 RNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMT 188
           +N+EH+LKT+F +YPKGP ++    D LG GIF +D +TW  QRKTA++EF +   R+  
Sbjct: 89  KNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 189 VESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 248
              +   +  R   +LE        VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 249 FAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELS 308
           FA AF+ ATEA++ RFI P F+W+  ++L LG+E  L  S+  +D + + VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQELL 268

Query: 309 AAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLL 368
           +  +   + +  DLL+ FM+ +D+    +S+ FLR + +NFILAGRDTSSVAL+WFFWL+
Sbjct: 269 SQRESGVQ-RHDDLLSRFMKKKDQS---YSETFLRHVALNFILAGRDTSSVALSWFFWLI 328

Query: 369 DQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPS 428
             +P VE+K + EIC ++ E           + L F  +E+ ++ YL AA+SE LRLYPS
Sbjct: 329 TTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEF--DEVDRLVYLKAALSETLRLYPS 388

Query: 429 VPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--DGRF 488
           VP D K VV DD+ PDGT +  G+ + Y+IYA GRM++ WG+DC EFKPERW+   DG+F
Sbjct: 389 VPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKF 448

Query: 489 MSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYMK 548
           ++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+TVA GH VE K++LT +MK
Sbjct: 449 VNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMK 508

Query: 549 HGLKVNLIKRE 553
           +GL VN+ KR+
Sbjct: 509 NGLLVNVHKRD 512

BLAST of Moc09g05760 vs. ExPASy TrEMBL
Match: A0A6J1DJX8 (cytochrome P450 86B1 OS=Momordica charantia OX=3673 GN=LOC111021175 PE=3 SV=1)

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH
Sbjct: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT
Sbjct: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR
Sbjct: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR
Sbjct: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF
Sbjct: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           KHGLKVNLIKREEGQIRKFMKLN
Sbjct: 541 KHGLKVNLIKREEGQIRKFMKLN 563

BLAST of Moc09g05760 vs. ExPASy TrEMBL
Match: A0A6J1GQ36 (cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 5.8e-295
Identity = 503/563 (89.34%), Postives = 529/563 (93.96%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFS+AIPFLHHKPMMDS NL SSSS+LP L+S+G FG   LLD QILEI FALFVFLAIH
Sbjct: 1   MFSHAIPFLHHKPMMDSGNL-SSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           VVRQ+RRYGLPVWP+VGMLP+LITAARGNLYEWIT+LLR HKGTFQF GPW SSLNCVVT
Sbjct: 61  VVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+FR
Sbjct: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           +MTVESLVELVH RLE VLED+ GR  AVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 QMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATE TVLRFITPTF+WK +RYLNLG+EKKLK SI+AVDEFAE+VI+TRKR
Sbjct: 241 EIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           ELS  AQPQAENQRSDLLTVFMRLRDEQ RPFSDKFLRDICVNFILAGRDTSSVAL+WFF
Sbjct: 301 ELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLD+NPDVE+K LAEI RIVGEREEA+T E RFDSL+FKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+DGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRF AFNGGPRLCLG+DFAYYQMKYVAASII RYRV V  GHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           K GLKVNLIKREEGQIRK++KLN
Sbjct: 541 KDGLKVNLIKREEGQIRKYVKLN 562

BLAST of Moc09g05760 vs. ExPASy TrEMBL
Match: A0A1S3CEY8 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103499965 PE=3 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.1e-292
Identity = 498/564 (88.30%), Postives = 524/564 (92.91%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNL-SSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAI 60
           MFS+A PFLHHKP+MDS NL SSSSSSLPP IS   FG + LLDVQILEI FALFVFL I
Sbjct: 25  MFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVI 84

Query: 61  HVVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVV 120
           HVVRQKRR+GLP+WP+VGMLPALITA RGNLYEWIT+LL RHKGTFQF GPWL+SLNCVV
Sbjct: 85  HVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVV 144

Query: 121 TADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKF 180
           TADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+F
Sbjct: 145 TADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 204

Query: 181 RRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 240
           R+MTVESLVEL H RLE VLED+AG+   VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL
Sbjct: 205 RQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 264

Query: 241 PEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRK 300
           PEIPFAKAFEDATE TV+RFITPTFIWKAMRYLN+G+EKKLK SIKAVDEFAENVI+TRK
Sbjct: 265 PEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRK 324

Query: 301 RELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWF 360
           RELS  A+ QA+N+RSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL+WF
Sbjct: 325 RELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF 384

Query: 361 FWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALR 420
           FWLLDQNPDVE+K LAEICRIVGEREEA+T ELRFDSL+FKPEE KKMEYLHAAISEALR
Sbjct: 385 FWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEXKKMEYLHAAISEALR 444

Query: 421 LYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDG 480
           LYPSVP DHKEVVEDD FPDGTILKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMRDG
Sbjct: 445 LYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDG 504

Query: 481 RFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFY 540
           RFMSES YRF AFNGGPRLCLGKDFAYYQMKYVAASII RYRV V AGHPVEPKL+LTFY
Sbjct: 505 RFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFY 564

Query: 541 MKHGLKVNLIKREEGQIRKFMKLN 564
           MK GLKVNLIKREEGQIRK+MKLN
Sbjct: 565 MKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of Moc09g05760 vs. ExPASy TrEMBL
Match: A0A6J1JMG1 (cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.7e-290
Identity = 496/563 (88.10%), Postives = 522/563 (92.72%), Query Frame = 0

Query: 1   MFSNAIPFLHHKPMMDSSNLSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIH 60
           MFS+AIPFLHHKP+MDS NL SSSS+LP ++S+  F    LLDVQILE+ FALFVFLAIH
Sbjct: 1   MFSHAIPFLHHKPIMDSGNL-SSSSALPAVVSAENFRLWPLLDVQILELSFALFVFLAIH 60

Query: 61  VVRQKRRYGLPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVT 120
           V+RQ+RRYGL VWP+VGMLP+LITAARGNLYEWIT+LLRRHKGTFQF GPW SSLNCVVT
Sbjct: 61  VIRQQRRYGLSVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCVVT 120

Query: 121 ADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFR 180
           ADPRNLEHLLKTKFP YPKG FFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+FR
Sbjct: 121 ADPRNLEHLLKTKFPYYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFR 180

Query: 181 RMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240
           +MTVESLVELVH RLE VLED+  R  AVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP
Sbjct: 181 QMTVESLVELVHSRLETVLEDFVERRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLP 240

Query: 241 EIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKR 300
           EIPFAKAFEDATE TVLRFITPTF+WK +RYLNLG+EKKLK SIK VDEFAE+VI+TRKR
Sbjct: 241 EIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIKTVDEFAEDVIQTRKR 300

Query: 301 ELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFF 360
           ELS  AQPQA NQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL+WFF
Sbjct: 301 ELSTTAQPQANNQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF 360

Query: 361 WLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRL 420
           WLLDQNPDVE K LAEI RIVGER EA+T E RFDSL+FKPEEIKKMEYLHAAISEALRL
Sbjct: 361 WLLDQNPDVENKILAEIYRIVGERGEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRL 420

Query: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGR 480
           YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM+DGR
Sbjct: 421 YPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGR 480

Query: 481 FMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYM 540
           FMSESAYRF AFNGGPRLCLG+DFAYYQMKYVAASII RYRV V  GHPVEPKLALTFYM
Sbjct: 481 FMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYM 540

Query: 541 KHGLKVNLIKREEGQIRKFMKLN 564
           K GLKVNLIKREEGQIRK+MKLN
Sbjct: 541 KDGLKVNLIKREEGQIRKYMKLN 562

BLAST of Moc09g05760 vs. ExPASy TrEMBL
Match: A0A5A7UTT4 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00200 PE=3 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 5.0e-286
Identity = 489/550 (88.91%), Postives = 513/550 (93.27%), Query Frame = 0

Query: 15  MDSSNL-SSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIHVVRQKRRYGLPVW 74
           MDS NL SSSSSSLPP IS   FG + LLDVQILEI FALFVFL IHVVRQKRR+GLP+W
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 75  PVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVTADPRNLEHLLKTK 134
           P+VGMLPALITA RGNLYEWIT+LL RHKGTFQF GPWL+SLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 135 FPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHR 194
           F SYPKG FFRDTVRDLLGDGIFGADDETWQRQRK ASIEFHSA+FR+MTVESLVELVH 
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

Query: 195 RLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 254
           RLE VLED+AG+   VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE
Sbjct: 181 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 240

Query: 255 ATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQ 314
            TV+RFITPTFIWKAMRYLN+G+EKKLK SIKAVDEFAENVI+TRKRELS  A+ QA+N+
Sbjct: 241 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 300

Query: 315 RSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKT 374
           RSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVAL+WFFWLLDQNPDVE+K 
Sbjct: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 360

Query: 375 LAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVE 434
           LAEICRIVGEREEA+T ELRFDSL+FKPEEIKKMEYLHAAISEALRLYPSVP DHKEVVE
Sbjct: 361 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420

Query: 435 DDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFN 494
           DD FPDGTILKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSES YRF AFN
Sbjct: 421 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 480

Query: 495 GGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKREE 554
           GGPRLCLGKDFAYYQMKYVAASII RYRV V AGHPVEPKL+LTFYMK GLKVNLIKREE
Sbjct: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 540

Query: 555 GQIRKFMKLN 564
           GQIRK+MKLN
Sbjct: 541 GQIRKYMKLN 550

BLAST of Moc09g05760 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 764.6 bits (1973), Expect = 5.5e-221
Identity = 374/538 (69.52%), Postives = 435/538 (80.86%), Query Frame = 0

Query: 22  SSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIHVVRQKRRYGLPVWPVVGMLPA 81
           SSSSS  PL+S   F  R   DVQILE+  A+FVF+AIH +RQK+  GLPVWP +GMLP+
Sbjct: 17  SSSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 82  LITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVTADPRNLEHLLKTKFPSYPKGP 141
           L    RGN+YEW++++L    GTFQF GPW SSLN  +T DPRN+EHLLK +F  +PKG 
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 142 FFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLED 201
           +FRD +RDLLGDGIF ADDETWQRQRKTASIEFHSAKFR++T +SL ELVH+RL  VLE 
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLET 196

Query: 202 YAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFIT 261
                + +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATEA V+RF+ 
Sbjct: 197 SVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVM 256

Query: 262 PTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVF 321
           PT +WK MRYL++G EKKLK SIK VD+FA+ VIRTRK+ELS   +     +RSDLLTVF
Sbjct: 257 PTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGE---TTKRSDLLTVF 316

Query: 322 MRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIV 381
           M LRDE+G  FSDKFLRDICVNFILAGRDTSSVAL+WFFWLL++NP+VEEK + E+C+I+
Sbjct: 317 MGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKIL 376

Query: 382 GEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGT 441
            +R++    E      +F PEEIKKM+YL AA+SEALRLYPSVPVDHKEV EDDVFPDGT
Sbjct: 377 RQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGT 436

Query: 442 ILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFNGGPRLCLG 501
           +LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+RDGRFMSESAY+F AFNGGPRLCLG
Sbjct: 437 MLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLG 496

Query: 502 KDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKREEGQIRKF 560
           KDFAYYQMK  AA+I+ RY+V V  GH VEPKLALT YMKHGL VNLI R   +I ++
Sbjct: 497 KDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of Moc09g05760 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-206
Identity = 345/490 (70.41%), Postives = 405/490 (82.65%), Query Frame = 0

Query: 70  LPVWPVVGMLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVTADPRNLEHL 129
           +PVWP+VGMLP+LI+A R N+YEW++++L    GTF+F GPW S+LNCVVT DPRN+EHL
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 130 LKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMTVESLVE 189
           LKT+F  YPKG +FR+T++DLLGDGIF  DD TWQRQRK AS+EFHSAKFR++T +SL E
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 190 LVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFE 249
           LVH RL  VLE        +DLQD+LLRLTFDNVCMIAFGVDPGCL   LPEIPFAKAFE
Sbjct: 121 LVHNRLLPVLE----TSGKIDLQDILLRLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAFE 180

Query: 250 DATEATVLRFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQ 309
           DATEATV+RF+ P F+WK MR LNLG EKKLK SI  VD+FAE VIRTRK+E+S   +  
Sbjct: 181 DATEATVVRFVMPKFVWKLMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMSLETEIA 240

Query: 310 AENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQNPDV 369
              +R DLLT+FM LRDE G+ FSDKFLRDICVNFILAGRDTSSVAL+WFFWL+++NP+V
Sbjct: 241 ---KRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFWLIEKNPEV 300

Query: 370 EEKTLAEICRIVGER-EEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPSVPVDH 429
           EEK +  IC+I+ +R +   T +      +F+PEEIKKM+YL AA+SE LRLYPSVPVDH
Sbjct: 301 EEKIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRLYPSVPVDH 360

Query: 430 KEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYR 489
           KEV+EDDVFPDGT LKKG K+IYAIYAMGRME IWGKDCREFKPERW+RDGR+MSESAY+
Sbjct: 361 KEVLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGRYMSESAYK 420

Query: 490 FAAFNGGPRLCLGKDFAYYQMKYVAASIIRRYRVTV--AAGHPVEPKLALTFYMKHGLKV 549
           F AFNGGPRLCLGKDFAYYQM+YVAA+II RY+V V    GH VEPK+ALT YMKHGLKV
Sbjct: 421 FTAFNGGPRLCLGKDFAYYQMRYVAAAIIYRYKVRVDDKGGHKVEPKMALTMYMKHGLKV 480

Query: 550 NLIKREEGQI 557
           N++KR   +I
Sbjct: 481 NMVKRSVSEI 483

BLAST of Moc09g05760 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 490.3 bits (1261), Expect = 2.0e-138
Identity = 238/517 (46.03%), Postives = 345/517 (66.73%), Query Frame = 0

Query: 44  VQILEIFFALFVFLAIHVVRQK--RRYGLPVWPVVGMLPALITAARGNLYEWITELLRRH 103
           +++ ++  AL     +  +R+K   + G  +WPV+G++P ++   + +L+ W T  + R 
Sbjct: 15  LRLSDVALALVGLFLLSYLREKLVSKGGPVMWPVLGIIP-MLALNKHDLFTWCTRCVVRS 74

Query: 104 KGTFQFHGPWLSSLNCVVTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDE 163
            GTF + G W      ++TADP N+EH+LKT F +YPKG F+R+  RDLL DGIF ADDE
Sbjct: 75  GGTFHYRGIWFGGAYGIMTADPANVEHILKTNFKNYPKGAFYRERFRDLLEDGIFNADDE 134

Query: 164 TWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVLLRLTFD 223
            W+ +R+ A  E HS++F   T  ++ +LV ++L  ++E+ +      DLQD+LLR TFD
Sbjct: 135 LWKEERRVAKTEMHSSRFLEHTFTTMRDLVDQKLVPLMENLSTSKRVFDLQDLLLRFTFD 194

Query: 224 NVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLGMEKKLK 283
           N+C+ AFGV PG L  GLPEIPFAKAFEDATE T+ RF+ P F+WK MR+L +G E+KL 
Sbjct: 195 NICISAFGVYPGSLETGLPEIPFAKAFEDATEYTLARFLIPPFVWKPMRFLGIGYERKLN 254

Query: 284 LSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVFMRLRDE------QGRPFSDK 343
            +++ V  FA   +R R+ ++         N  +DLL+  M+   E      +G  FSDK
Sbjct: 255 NAVRIVHAFANKTVRERRNKMRKLGN---LNDYADLLSRLMQREYEKEEDTTRGNYFSDK 314

Query: 344 FLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFD 403
           + R+ C +FI+AGRDT+SVAL WFFWL+ ++P+VE++ L EI  I  +    +T +    
Sbjct: 315 YFREFCTSFIIAGRDTTSVALVWFFWLVQKHPEVEKRILREIREIKRKLTTQETED---- 374

Query: 404 SLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYA 463
              F+ E+ ++M YL AA++E+LRLYPSVP++ K+ +EDDV PDGT +KKG +I Y++Y+
Sbjct: 375 --QFEAEDFREMVYLQAALTESLRLYPSVPMEMKQALEDDVLPDGTRVKKGARIHYSVYS 434

Query: 464 MGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAAS 523
           MGR+E+IWGKD  EFKPERW+++GR +SE  +++  FNGGPRLC+GK FAY QMK VAA+
Sbjct: 435 MGRIESIWGKDWEEFKPERWIKEGRIVSEDQFKYVVFNGGPRLCVGKKFAYTQMKMVAAA 494

Query: 524 IIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKRE 553
           I+ RY V V  G  + PKL  T YMK+G+ V L  R+
Sbjct: 495 ILMRYSVKVVQGQEIVPKLTTTLYMKNGMNVMLQPRD 521

BLAST of Moc09g05760 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 476.5 bits (1225), Expect = 3.0e-134
Identity = 251/517 (48.55%), Postives = 339/517 (65.57%), Query Frame = 0

Query: 42  LDVQILEIFFALFVFLAIHVVRQKRRY-GLPVWPVVGMLPALITAARGNLYEWITELLRR 101
           +D     I   L V   I  V  +R Y G  VWP+VG LPALIT A   ++++I + LR 
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 102 HKGTFQ---FHGPWLSSL--NCVVTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGI 161
             GT+Q   F  P+L+    +  VT DP+NLEH+LKT+F +YPKGP ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 162 FGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEGVLEDYAGRPAAVDLQDVL 221
           F +D +TW+ QRKTA++EF +   R+     +   +  RL  +LE    R   +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 222 LRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVLRFITPTFIWKAMRYLNLG 281
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATEAT+ RFI P FIWK  ++L LG
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 282 MEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDLLTVFMRLRDEQGRPFSDK 341
           +E  +  SI  VD +   +I TRK EL    Q Q E++  DLL+ FM+ ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLEL--LGQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 342 FLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKTLAEICRIVGEREEAQTGELRFD 401
           +L+ + +NFILAGRDTSSVA++WFFWL+  NP VEEK + EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 402 SLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYA 461
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 462 MGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFNGGPRLCLGKDFAYYQMKYVAAS 521
           +GRM+ IWG+DC EFKPERW+ + R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 522 IIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNLIKRE 553
           I+ R+R+TVA GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

BLAST of Moc09g05760 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 476.1 bits (1224), Expect = 3.9e-134
Identity = 235/535 (43.93%), Postives = 346/535 (64.67%), Query Frame = 0

Query: 20  LSSSSSSLPPLISSGKFGFRQLLDVQILEIFFALFVFLAIHVVRQK--RRYGLPVWPVVG 79
           +S  SSS+P  ++   +    L DV +      LFVF     VR+K  ++ G  +WPV G
Sbjct: 1   MSEISSSMP--LTERVYNHLCLSDVSL--ALLGLFVFCC---VREKVTKKLGPTIWPVFG 60

Query: 80  MLPALITAARGNLYEWITELLRRHKGTFQFHGPWLSSLNCVVTADPRNLEHLLKTKFPSY 139
           + P      R ++Y W T  L++ +GTF ++G WL      VT  P N+E++LKT F ++
Sbjct: 61  ITPEFF-FHRNDVYGWATRCLKKCRGTFLYNGIWLGGSYGAVTCVPANVEYMLKTNFKNF 120

Query: 140 PKGPFFRDTVRDLLGDGIFGADDETWQRQRKTASIEFHSAKFRRMTVESLVELVHRRLEG 199
           PKG FF++   DLL DGIF AD E+W+ QR+    E HS +F   + ++  +LV ++L  
Sbjct: 121 PKGAFFKERFNDLLEDGIFNADAESWKEQRRIIITEMHSTRFVEHSFQTTQDLVRKKLLK 180

Query: 200 VLEDYAGRPAAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEATVL 259
           V+E +A    A DLQDVLLRLTFDN+C+   G DPG L   LP +PFA+AFE+ATE+T+ 
Sbjct: 181 VMESFARSQEAFDLQDVLLRLTFDNICIAGLGDDPGTLDSDLPLVPFAQAFEEATESTMF 240

Query: 260 RFITPTFIWKAMRYLNLGMEKKLKLSIKAVDEFAENVIRTRKRELSAAAQPQAENQRSDL 319
           RF+ P FIWK +++ ++G EK L+ ++  V EF + ++  R  +L    +      RSD+
Sbjct: 241 RFMIPPFIWKPLKFFDIGYEKGLRKAVDVVHEFVDKMVVDRICKLK---EEGTLGNRSDV 300

Query: 320 LTVFMRLRD----EQGRPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQNPDVEEKT 379
           L+  + +      ++  P + KF R  C +FILAGRDTSSVALTWFFW++ ++P+VE K 
Sbjct: 301 LSRIIEIESHKTTDEKDPSTIKFFRQFCTSFILAGRDTSSVALTWFFWVIQKHPEVENKI 360

Query: 380 LAEICRIVGEREEAQTGELRFDSLMFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVE 439
           + EI  I+ +R ++ T +   +  +F  +E+  M YL AA+SE +RLYP +P++ K+ +E
Sbjct: 361 IREISEILRQRGDSPTSK---NESLFTVKELNDMVYLQAALSETMRLYPPIPMEMKQAIE 420

Query: 440 DDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESAYRFAAFN 499
           DDVFPDGT ++KG+++ +A YAMGRME+IWGKDC  FKPERW++ G F+++  +++  FN
Sbjct: 421 DDVFPDGTFIRKGSRVYFATYAMGRMESIWGKDCESFKPERWIQSGNFVNDDQFKYVVFN 480

Query: 500 GGPRLCLGKDFAYYQMKYVAASIIRRYRVTVAAGHPVEPKLALTFYMKHGLKVNL 549
            GPRLCLGK FAY QMK +AAS++ RY + VA  H V P++  T YM+HGLKV +
Sbjct: 481 AGPRLCLGKTFAYLQMKTIAASVLSRYSIKVAKDHVVVPRVTTTLYMRHGLKVTI 521

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153724.10.0e+00100.00cytochrome P450 86B1 [Momordica charantia][more]
KAG7014270.15.4e-29589.17Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023549182.17.1e-29589.20cytochrome P450 86B1-like [Cucurbita pepo subsp. pepo][more]
XP_022954102.11.2e-29489.34cytochrome P450 86B1-like [Cucurbita moschata][more]
XP_038899430.11.9e-29289.34cytochrome P450 86B1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FMY17.8e-22069.52Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD64.2e-13348.55Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Q9LMM14.1e-12848.78Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
B3RFJ69.1e-12847.74Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
O230661.6e-12747.66Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DJX80.0e+00100.00cytochrome P450 86B1 OS=Momordica charantia OX=3673 GN=LOC111021175 PE=3 SV=1[more]
A0A6J1GQ365.8e-29589.34cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=... [more]
A0A1S3CEY82.1e-29288.30LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1JMG15.7e-29088.10cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1[more]
A0A5A7UTT45.0e-28688.91Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT5G23190.15.5e-22169.52cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.11.7e-20670.41Cytochrome P450 superfamily protein [more]
AT1G24540.12.0e-13846.03cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G63710.13.0e-13448.55cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
AT1G13140.13.9e-13443.93cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 356..382
score: 32.63
coord: 65..84
score: 19.96
coord: 336..353
score: 28.15
coord: 499..522
score: 19.44
coord: 411..429
score: 30.58
coord: 489..499
score: 41.74
IPR001128Cytochrome P450PRINTSPR00385P450coord: 412..423
score: 44.94
coord: 347..364
score: 36.92
coord: 490..499
score: 45.76
coord: 499..510
score: 35.08
IPR001128Cytochrome P450PFAMPF00067p450coord: 70..536
e-value: 6.9E-74
score: 249.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 52..556
e-value: 2.1E-104
score: 351.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 69..551
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 34..552
NoneNo IPR availablePANTHERPTHR24296:SF177CYTOCHROME P450 86B1-LIKEcoord: 34..552
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 492..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g05760.1Moc09g05760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen