Moc09g05220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g05220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin ligase BIG BROTHER
Location	chr9: 4017976 .. 4020450 (+)
RNA-Seq Expression	Moc09g05220
Synteny	Moc09g05220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTCTTTGAAGGGCTCCATCACCACCAGGTACCTGCTTATGGCAATGCTGGGCCATTGCTTGATCAGGTATTCCGAAAAGTCGAAACTTTTATGAAAAACGATGAAAATTTTCCGATACTTGAACTCAGGTCTTGTAAGTTGGTAGTAATTAGTATTGGAAATGTATGGTACTTTGGTTCTTAGGGAAATGCATACTGGTCGATGAATATGCAATGTTACAAATTTGGAGTATCCGATCCCGGGAATACATATTACGGCCATTCCGAGGAAAGTCATCATTTGCCACCTTCAATGGATATTGGTGAAAGGGGGTGGGAGTATCCTTCAACCGTGACTGTCGAAAATCCTGAACCGACGTATGCACCATCGGTGGAGGAGGATGTTGTAGATGCTCATTTTATACCCGAAGAAGAATGTAAGTGTTGGCAGATCTGATAATGATACCAAGTCTGTTCAAATGATAACGTTGATTTTTTTTTTTTTTGCAAACTGTCGAGTTTAATTGTCGAAAATGTTTGAAGAACGGCATAGTTGCAGATCTTATCATCAGCTTTTTACTTTGTTTTCCTGTTTTGCAGGTGATTTCAGTCATCAAGAGGAGGAGACCAGTACATCTCAAGTATGATTCTTCGTCGTTGATTTTGCAATATGCCTCTTCTTTTATTTCCATACAAAAGTCACCAACAAGTTTTAGGATCATCATCTACCTAATTCCTCTTACGAATTGTGGTTTAATCGAAACGCGTATCTGTTTATTAATAAATTGGGACATAACCTCGTTATCATCAAAGATTGTCAAATGTTTATATACTGTTGTACTAAAGTTATCAAGTGGCTGATTGAATTACTTTTGTCCTCAACTTTAACTTTTGTCGCTTTTACACTTGAGAATACACCTCTTTTAGTTCAAAACGCATCGAATCCATCTCATTTTGTTCGTCTCCTCAGGTTGTATGGCAAGATGAGATTGATCCTGATCACATGACCTATGAGGTAACGTTATCTTTTAGAAACACAATATGATAGAGATTCTCATTCCGTTTTCTAGACGATACTTGTTTCTATGACGACTTGTAGGATTGTTCCATGAAGGAATGAAAAGATCATTTGGCATACGATTCTCATGCTTTAAGATGTTTCCCATCTTGAGTAATATATTTGCGATTAGTAACCACGACCACTGCGATGATTTCGAGAGAAAGAGGAAAAATTATGCTATGACAACATTATAACCTTAAAATCTTCATACCAAACCAGTTTTAGATCAAGGATCAATCATCTTTGTTTTTTGTTTTTGATCAAAATTATTGCTGACATAAGAAGCTTTTAGGCTCAATTGCTTTAACTTTTAACAGTTTGTGATAAAAAGTTTTTAGGCTCAATTTTCAAACAAGCCCCTAATTCCCTTTGTAAATTACCATTTGAACTATGTGTGGCTGTCGAATAAATGAGAACCAAAGGACTTGACTGTAAAAAGAAAAAAGCTTCAATGGGAATGTCTGGAGCCATAACTGGACATTTTACTACACGATTTTACCAGGAACTATTGGACTTGGGCGAATCGGTTGGAACTCAAAGTCGGGGGCTTTCTGAAGAACAAATAAGTCTGCTTCCGACTGCGAAATGCAAGTTAACGAGCTTTTTCTCGAGAAAAAAGCCAGAGGAAAGGTATGGTTTTTGACATATGTTGCATTAGAGTAAACTCGCTCAATGTTTGGTTAATCTGCTTTACTTCGAGGGCATAGGAACATTCTTGTCAAAATAACTTGTAATGATCTATTGGTAATCGGTCCGGAACATTTTAAACAACAGTTTCTATGAATTAGGATACAATGTCGGTCTTTATATGATGTTTGATTCTGATACGTTTACCTTAGAGTCATATGCATCCATTTTTCCGTCCTAGTACGCGAGATAGTACTGTCAAATGAACCTATACTCTTTGATGTCCTTGGTGTGTTGTGTAGCCATGTCGAATCTCTAACATATGATACAAATATTCCACAAATCTCCAAGCTTAAATACCATGGTTAGAAAGCATTAAAAAACGCTAATAATTCGGTTTAGCAGTAGATTGTTCTCGGAGTTATTTCTTATGTTGCTGATTGTAGATTAGTACTTTCTCGGTACGGTATTTACATTCAGGATCTCGGTTTAATATTGATTGTACTTGTTTCGTTTCGACTGCAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAGTTGCCCTGCAAGCATTTCTACCATAACAAATGCATAACCAAATGGCTTACTATAAACAAAGTCAGTTCTTTTCTTGACTTTCTTATATCTCCCTATTTCCACCATTTCTACTTCTAATTTCCTTTCATCTCACAAATCGTCATTGTCATTGTCAGATATGTCCGATCTGCAACATCGAGGTGTTTGGAGACGAAGGCGTGAATGCGTCTGGCAGCGATAACCGACAGTAG

mRNA sequence

ATGGATTTCTTTGAAGGGCTCCATCACCACCAGGTACCTGCTTATGGCAATGCTGGGCCATTGCTTGATCAGGGAAATGCATACTGGTCGATGAATATGCAATGTTACAAATTTGGAGTATCCGATCCCGGGAATACATATTACGGCCATTCCGAGGAAAGTCATCATTTGCCACCTTCAATGGATATTGGTGAAAGGGGGTGGGAGTATCCTTCAACCGTGACTGTCGAAAATCCTGAACCGACGTATGCACCATCGGTGGAGGAGGATGTTGTAGATGCTCATTTTATACCCGAAGAAGAATGTGATTTCAGTCATCAAGAGGAGGAGACCAGTACATCTCAAGTTGTATGGCAAGATGAGATTGATCCTGATCACATGACCTATGAGGAACTATTGGACTTGGGCGAATCGGTTGGAACTCAAAGTCGGGGGCTTTCTGAAGAACAAATAAGTCTGCTTCCGACTGCGAAATGCAAGTTAACGAGCTTTTTCTCGAGAAAAAAGCCAGAGGAAAGATTAGTACTTTCTCGGTACGGTATTTACATTCAGGATCTCGGTTTAATATTGATTGTACTTGTTTCGTTTCGACTGCAGATGTGTGATTTGCCAAATGAGATATGTCCGATCTGCAACATCGAGGTGTTTGGAGACGAAGGCGTGAATGCGTCTGGCAGCGATAACCGACAGTAG

Coding sequence (CDS)

ATGGATTTCTTTGAAGGGCTCCATCACCACCAGGTACCTGCTTATGGCAATGCTGGGCCATTGCTTGATCAGGGAAATGCATACTGGTCGATGAATATGCAATGTTACAAATTTGGAGTATCCGATCCCGGGAATACATATTACGGCCATTCCGAGGAAAGTCATCATTTGCCACCTTCAATGGATATTGGTGAAAGGGGGTGGGAGTATCCTTCAACCGTGACTGTCGAAAATCCTGAACCGACGTATGCACCATCGGTGGAGGAGGATGTTGTAGATGCTCATTTTATACCCGAAGAAGAATGTGATTTCAGTCATCAAGAGGAGGAGACCAGTACATCTCAAGTTGTATGGCAAGATGAGATTGATCCTGATCACATGACCTATGAGGAACTATTGGACTTGGGCGAATCGGTTGGAACTCAAAGTCGGGGGCTTTCTGAAGAACAAATAAGTCTGCTTCCGACTGCGAAATGCAAGTTAACGAGCTTTTTCTCGAGAAAAAAGCCAGAGGAAAGATTAGTACTTTCTCGGTACGGTATTTACATTCAGGATCTCGGTTTAATATTGATTGTACTTGTTTCGTTTCGACTGCAGATGTGTGATTTGCCAAATGAGATATGTCCGATCTGCAACATCGAGGTGTTTGGAGACGAAGGCGTGAATGCGTCTGGCAGCGATAACCGACAGTAG

Protein sequence

MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRYGIYIQDLGLILIVLVSFRLQMCDLPNEICPICNIEVFGDEGVNASGSDNRQ

Homology

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BLAST of Moc09g05220 vs. NCBI nr
Match: XP_022148735.1 (E3 ubiquitin ligase BIG BROTHER [Momordica charantia])

HSP 1 Score: 407.9 bits (1047), Expect = 5.7e-110
Identity = 203/234 (86.75%), Postives = 208/234 (88.89%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS
Sbjct: 25  MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQD 120
           MDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQD
Sbjct: 85  MDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQD 144

Query: 121 EIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRYG 180
           EIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEER V+ +  
Sbjct: 145 EIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERCVICQMR 204

Query: 181 IYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDNRQ 231
            Y +    I +    F    C       N+ICPICNIEVFGDEGVNASGSDNRQ
Sbjct: 205 -YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDEGVNASGSDNRQ 257

[Close]

BLAST of Moc09g05220 vs. NCBI nr
Match: XP_038899262.1 (E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899264.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida])

HSP 1 Score: 349.0 bits (894), Expect = 3.2e-92
Identity = 182/235 (77.45%), Postives = 194/235 (82.55%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFFEGLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 25  MDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYPSTV-TVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP++V  VE PEPTYAPSVEEDVVDAH IP EECD SHQ EETSTSQV WQ
Sbjct: 85  MDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAHSIP-EECDLSHQ-EETSTSQVTWQ 144

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+ KLTSFFSRKKPEER V+ + 
Sbjct: 145 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARFKLTSFFSRKKPEERCVICQM 204

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDNRQ 231
             Y +    I +    F    C       N+ICPICNIEVFGD  +NASG+DNRQ
Sbjct: 205 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSMNASGNDNRQ 256

[Close]

BLAST of Moc09g05220 vs. NCBI nr
Match: TYK10442.1 (E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-89
Identity = 177/233 (75.97%), Postives = 190/233 (81.55%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 25  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S V VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV+WQ
Sbjct: 85  MDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVIWQ 144

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 145 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 204

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 205 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Moc09g05220 vs. NCBI nr
Match: XP_008461284.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis melo])

HSP 1 Score: 339.0 bits (868), Expect = 3.3e-89
Identity = 177/233 (75.97%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 19  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 78

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S V VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV WQ
Sbjct: 79  MDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVTWQ 138

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 139 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 198

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 199 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 248

[Close]

BLAST of Moc09g05220 vs. NCBI nr
Match: XP_008461282.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_008461283.1 PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo])

HSP 1 Score: 339.0 bits (868), Expect = 3.3e-89
Identity = 177/233 (75.97%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 25  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S V VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV WQ
Sbjct: 85  MDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVTWQ 144

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 145 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 204

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 205 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Moc09g05220 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-33
Identity = 96/227 (42.29%), Postives = 129/227 (56.83%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGN-TYYGHSEESHHLPP 60
           MDF+ G     +  Y +A  +  Q N YW+MN   YKFG S   N ++YG  + + HL  
Sbjct: 27  MDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYGSYDMNDHL-S 86

Query: 61  SMDIGERGWEYPSTVTV-ENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVW 120
            M IG   W+Y   V V ++PE T A SV+    D H    EEC     E +  + QV W
Sbjct: 87  RMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEH-SEAEEC--IANEHDPDSPQVSW 146

Query: 121 QDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLS- 180
           QD+IDPD MTYEEL++LGE+VGT+SRGLS+E I  LPT K K  S FSRK+  ER V+  
Sbjct: 147 QDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQ 206

Query: 181 -RYGIYIQDLGL-ILIVLVSFRLQMCDLPNEICPICNIEVFGDEGVN 223
            +Y I  + + L    V  S  +      N++CP+CN EVFG+  ++
Sbjct: 207 LKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPSIH 248

[Close]

BLAST of Moc09g05220 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.0e-10
Identity = 45/112 (40.18%), Postives = 64/112 (57.14%), Query Frame = 0


Query: 108 EEETSTSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSR 167
           E+E+ TSQ  W DE+DPD ++YEELL LG+ VGT+SRGLS + I+ LP+ + K     ++
Sbjct: 225 EDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQ 284

Query: 168 KKPEERLVLSRYGIYIQDLGLILIVLVSFRLQMCD----LPNEICPICNIEV 216
               E  V+ R   Y  D  LIL+         C       N++CP+C+ EV
Sbjct: 285 NGTNESCVICRLD-YEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of Moc09g05220 vs. ExPASy TrEMBL
Match: A0A6J1D3R4 (E3 ubiquitin ligase BIG BROTHER OS=Momordica charantia OX=3673 GN=LOC111017324 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 203/234 (86.75%), Postives = 208/234 (88.89%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS
Sbjct: 25  MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQD 120
           MDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQD
Sbjct: 85  MDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQD 144

Query: 121 EIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRYG 180
           EIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEER V+ +  
Sbjct: 145 EIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERCVICQMR 204

Query: 181 IYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDNRQ 231
            Y +    I +    F    C       N+ICPICNIEVFGDEGVNASGSDNRQ
Sbjct: 205 -YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDEGVNASGSDNRQ 257

[Close]

BLAST of Moc09g05220 vs. ExPASy TrEMBL
Match: A0A5D3CGQ8 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00890 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 7.1e-90
Identity = 177/233 (75.97%), Postives = 190/233 (81.55%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 25  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S V VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV+WQ
Sbjct: 85  MDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVIWQ 144

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 145 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 204

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 205 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Moc09g05220 vs. ExPASy TrEMBL
Match: A0A1S3CED1 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.6e-89
Identity = 177/233 (75.97%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 25  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 84

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S V VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV WQ
Sbjct: 85  MDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVTWQ 144

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 145 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 204

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 205 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 254

[Close]

BLAST of Moc09g05220 vs. ExPASy TrEMBL
Match: A0A1S3CES7 (E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.6e-89
Identity = 177/233 (75.97%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 19  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 78

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S V VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV WQ
Sbjct: 79  MDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVTWQ 138

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 139 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 198

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 199 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 248

[Close]

BLAST of Moc09g05220 vs. ExPASy TrEMBL
Match: A0A0A0K8C4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.5e-89
Identity = 176/233 (75.54%), Postives = 189/233 (81.12%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPS 60
           MDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD GNTYYGHSEESHHLPPS
Sbjct: 19  MDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPS 78

Query: 61  MDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQ 120
           MD+GER WEYP S + VE PEP+YAPSVEEDVVDAH IP EECD SH+ EETSTSQV WQ
Sbjct: 79  MDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIP-EECDLSHR-EETSTSQVTWQ 138

Query: 121 DEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLSRY 180
           DEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+CKLTSFFSRKK +ER V+ + 
Sbjct: 139 DEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQM 198

Query: 181 GIYIQDLGLILIVLVSFRLQMCDLP----NEICPICNIEVFGDEGVNASGSDN 229
             Y +    I +    F    C       N+ICPICNIEVFGD   NAS SDN
Sbjct: 199 R-YKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN 248

[Close]

BLAST of Moc09g05220 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-34
Identity = 96/227 (42.29%), Postives = 129/227 (56.83%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGN-TYYGHSEESHHLPP 60
           MDF+ G     +  Y +A  +  Q N YW+MN   YKFG S   N ++YG  + + HL  
Sbjct: 27  MDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYGSYDMNDHL-S 86

Query: 61  SMDIGERGWEYPSTVTV-ENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVW 120
            M IG   W+Y   V V ++PE T A SV+    D H    EEC     E +  + QV W
Sbjct: 87  RMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEH-SEAEEC--IANEHDPDSPQVSW 146

Query: 121 QDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLS- 180
           QD+IDPD MTYEEL++LGE+VGT+SRGLS+E I  LPT K K  S FSRK+  ER V+  
Sbjct: 147 QDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQ 206

Query: 181 -RYGIYIQDLGL-ILIVLVSFRLQMCDLPNEICPICNIEVFGDEGVN 223
            +Y I  + + L    V  S  +      N++CP+CN EVFG+  ++
Sbjct: 207 LKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPSIH 248

[Close]

BLAST of Moc09g05220 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-34
Identity = 96/227 (42.29%), Postives = 129/227 (56.83%), Query Frame = 0


Query: 1   MDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGN-TYYGHSEESHHLPP 60
           MDF+ G     +  Y +A  +  Q N YW+MN   YKFG S   N ++YG  + + HL  
Sbjct: 27  MDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTNAYKFGFSGSDNASFYGSYDMNDHL-S 86

Query: 61  SMDIGERGWEYPSTVTV-ENPEPTYAPSVEEDVVDAHFIPEEECDFSHQEEETSTSQVVW 120
            M IG   W+Y   V V ++PE T A SV+    D H    EEC     E +  + QV W
Sbjct: 87  RMSIGRTNWDYHPMVNVADDPENTVARSVQIGDTDEH-SEAEEC--IANEHDPDSPQVSW 146

Query: 121 QDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERLVLS- 180
           QD+IDPD MTYEEL++LGE+VGT+SRGLS+E I  LPT K K  S FSRK+  ER V+  
Sbjct: 147 QDDIDPDTMTYEELVELGEAVGTESRGLSQELIETLPTKKYKFGSIFSRKRAGERCVICQ 206

Query: 181 -RYGIYIQDLGL-ILIVLVSFRLQMCDLPNEICPICNIEVFGDEGVN 223
            +Y I  + + L    V  S  +      N++CP+CN EVFG+  ++
Sbjct: 207 LKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSEVFGEPSIH 248

[Close]

BLAST of Moc09g05220 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.0e-11
Identity = 45/112 (40.18%), Postives = 64/112 (57.14%), Query Frame = 0


Query: 108 EEETSTSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSR 167
           E+E+ TSQ  W DE+DPD ++YEELL LG+ VGT+SRGLS + I+ LP+ + K     ++
Sbjct: 225 EDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQ 284

Query: 168 KKPEERLVLSRYGIYIQDLGLILIVLVSFRLQMCD----LPNEICPICNIEV 216
               E  V+ R   Y  D  LIL+         C       N++CP+C+ EV
Sbjct: 285 NGTNESCVICRLD-YEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of Moc09g05220 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 4.8e-06
Identity = 44/138 (31.88%), Postives = 73/138 (52.90%), Query Frame = 0


Query: 87  VEEDVVDAHFIPEEECDFSHQEEETSTSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGL 146
           V+ED ++  F+ ++E + + +EE+    +   +DEIDPD ++YEEL+ LG+ +G ++RGL
Sbjct: 81  VDEDEIN-EFLEDQESNSNLEEEDDFLEE---EDEIDPDQLSYEELIALGDFIGVENRGL 140

Query: 147 SEEQISLLPTAKCKLTSFFSRKKPE-ERLVLSRYGIYIQDLGLILIVLVSFRLQMCDLP- 206
           +  +IS    A    T  FS  K E +R V+ +     ++    L+V     L+ CD P 
Sbjct: 141 TPIEISTCLNAS---TYVFSHNKNEIDRCVVCQMEFEERE---SLVV-----LRPCDHPY 200

Query: 207 -----------NEICPIC 212
                       +ICPIC
Sbjct: 201 HSECITKWLETKKICPIC 203

[Close]

BLAST of Moc09g05220 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-05
Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = 0


Query: 104 FSHQEEETSTSQVVWQDE-IDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAK 159
           ++H    +S S     DE  DP++MTYEEL +LG+SVG   +GLS+E+IS L T K
Sbjct: 155 YTHTHSPSSISHNPTDDENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHK 210

The following BLAST results are available for this feature:

BLAST of Moc09g05220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148735.1	5.7e-110	86.75	E3 ubiquitin ligase BIG BROTHER [Momordica charantia]	[more]
XP_038899262.1	3.2e-92	77.45	E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 u...	[more]
TYK10442.1	1.5e-89	75.97	E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008461284.1	3.3e-89	75.97	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis melo]	[more]
XP_008461282.1	3.3e-89	75.97	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_0084612...	[more]

BLAST of Moc09g05220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	2.0e-33	42.29	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	7.0e-10	40.18	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]

BLAST of Moc09g05220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3R4	2.8e-110	86.75	E3 ubiquitin ligase BIG BROTHER OS=Momordica charantia OX=3673 GN=LOC111017324 P...	[more]
A0A5D3CGQ8	7.1e-90	75.97	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CED1	1.6e-89	75.97	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A1S3CES7	1.6e-89	75.97	E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A0A0K8C4	3.5e-89	75.54	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 P...	[more]

BLAST of Moc09g05220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	1.4e-34	42.29	RING/U-box superfamily protein	[more]
AT3G63530.2	1.4e-34	42.29	RING/U-box superfamily protein	[more]
AT3G19910.1	5.0e-11	40.18	RING/U-box superfamily protein	[more]
AT3G47180.1	4.8e-06	31.88	RING/U-box superfamily protein	[more]
AT5G52140.1	1.4e-05	48.21	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..220
None	No IPR available	PANTHER	PTHR46400:SF3	E3 UBIQUITIN-PROTEIN LIGASE BIG BROTHER	coord: 1..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g05220.1	Moc09g05220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048437	floral organ development
biological_process	GO:0008285	negative regulation of cell population proliferation
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:1900057	positive regulation of leaf senescence
biological_process	GO:0051865	protein autoubiquitination
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity