Moc09g04970 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g04970
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionTP_methylase domain-containing protein
Locationchr9: 3858029 .. 3860299 (-)
RNA-Seq ExpressionMoc09g04970
SyntenyMoc09g04970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCGCGTTTACGACCTCCAATCGCTTTCATCGCCGTTTTCGTCCCGCCCAAGAACACCCAGGACCCCAATTTTCAAACCCATCTCCTCAATCCACTGTTCCTCCTCTTCTTCCCCATTTACGGAGAAACATTCCATCAAGAGATACCAAAGAGACGATTGGGTGTACAAGAACCAAGCGGACCAAGCTTCAACTGCTCTGTCGTGTTCCGTTCCGTGCGATTCCGACTCAATACGGCAGGACGACATCGCTTTGCAGCTGCCGGAGCTGAAGAGGTTGCTGATGGTGCTGAGGGAGAAGAGGGTCAATGGTGGGTGCGATGATGGAGGAGAATGTGGGCCTGGGAATGTATTTCTAGTGGGGACTGGCCCTGGAGACCCTGAACTTTTGACATTGAAGGCTGTGAAAGTGATTCAGAGTGCCGATTTGCTTCTGTATGATCGTTTGGTGTCTAATGATGTGCTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTCGGCAAAACGGCAGGCTACCATAGCAGAACCCAGGTGAATGTTTCTTTGTGATTTTATCTATCTGAGTATTGAGTGTTTGAGTCTTGTTGCTGAATGGGAATTGGTTTTAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTAGTGAGACTTAAAGGAGGAGATCCTCTTGTAAGTGGAAACTTTGAATGGTTAAACTGCAATTGAATATGGTTTTGTCATTCAAGTGGTTTTTTTAGTGGCATATCCTGAAATTTTTGTCATTGTACTTTATAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAAAAAGGGATTCAAGTAAAAATTGTTCCTGGTAAGCCTATTTCCGAATGCTGTTTCAGTCCTTTAAGTGTTTCCATGAAGCTTAACTTAATGTGTTCTGCATCACCATTTGTTTGAAAGATTTGGTTGTTTGAGGGTATTTTCATGACTAAAAGCAAATGACTTGAAGAGAATAGGTCCAAACCATGGTTGCCACCTACGTAAGATTTAATATCCTGAGTTTCTAGGTTTTAATATCCCGAGTTTCATTGACAATCAAATGTAATAGAATCAGAGTTAGAAAATTGTTTCATAGATTAGTCGAGGTTTGCACAACCTCGATGTCTGAAGGACGTGTATGGACCTGAACTACCGAGCCTTTATTTCTTTTTGAATCATGATACCGCCTCCTGTTTTGGATGGCAAAGTTTTCCTAATCTGTCAGCAGCCACAAGTAACATGAGTCTGAGGTTGATTGCTGATTACAATGTTGCTTGGCTTTGCTTCATGCTAGGAATCAGTAAATGAATTATCATGAATTTGCTGCATTTTGATTCTCAGTGTCACGTCTATGCACCACCATTTTAAGGAGAGTATTCTTAGAGTAACCTCGTCTAAGAACACATGTGTTTGCCTTGCAGGTATAACTGCTGCTTCAGGTATAGCAGCTGAATTGGGGATTCCTTTAACGCACAGGGGTGTTGCAACGAACGTCAGGTTCCTCACTGGTCACTCTAGAAAGGGTGGAACGGATCCTCTATTTGTGGCAGAAAATGCTGCTGATCTGGATTCAACTCTGGTTGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCACGGTCTGCCACCCGATACCCCAGCTGCTGCCGTTGAACGAGGGACAACGCCCCAACAAAGAACTGTAAGCACAATTCTCTGTTGCTTTCTACTTTTGTCGGGCTTGGTTCAACCATATACAGCTTCTTCAAGAAGTCTCATGAGAGTCCTAATGCCTTGTCATTCCAGTTCTCAACTTTGCTCGACTTCTTTTGCGTTTCATCGATCGTTTTCTATGAGAAAGAAAAAAAAATCTTAAATGGGATCTGATCTATAGATTGCTCTCGAGTTCAGGAGACCTCTTGTAGGTCTATTTTCTTATCTGCGTTGGCTTCTGTACTTCATTAGCTATGTTCTCGAGAATGCCCGCTTCTAGACGTTTTTATCGAGGGGCGGGGCATGTTTGCTTGGCCTTTCATTTCATTTTGCTGGTGCCTATAACAAACTTTCCATGGATCAACTACAAATTATGCAGGTTTTTGCAGAACTGAAGGACCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTAATCATCATTGGAAAAGTGGTTTCTCTCTCACCGCATTGGTCTCTTTCTTCCAAAGAAGCCTCCTGTTTGGTGGAGGCTTGA

mRNA sequence

ATGGCTCGCGTTTACGACCTCCAATCGCTTTCATCGCCGTTTTCGTCCCGCCCAAGAACACCCAGGACCCCAATTTTCAAACCCATCTCCTCAATCCACTGTTCCTCCTCTTCTTCCCCATTTACGGAGAAACATTCCATCAAGAGATACCAAAGAGACGATTGGGTGTACAAGAACCAAGCGGACCAAGCTTCAACTGCTCTGTCGTGTTCCGTTCCGTGCGATTCCGACTCAATACGGCAGGACGACATCGCTTTGCAGCTGCCGGAGCTGAAGAGGTTGCTGATGGTGCTGAGGGAGAAGAGGGTCAATGGTGGGTGCGATGATGGAGGAGAATGTGGGCCTGGGAATGTATTTCTAGTGGGGACTGGCCCTGGAGACCCTGAACTTTTGACATTGAAGGCTGTGAAAGTGATTCAGAGTGCCGATTTGCTTCTGTATGATCGTTTGGTGTCTAATGATGTGCTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTCGGCAAAACGGCAGGCTACCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTAGTGAGACTTAAAGGAGGAGATCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAAAAAGGGATTCAAGTAAAAATTGTTCCTGGTATAACTGCTGCTTCAGGTATAGCAGCTGAATTGGGGATTCCTTTAACGCACAGGGGTGTTGCAACGAACGTCAGGTTCCTCACTGGTCACTCTAGAAAGGGTGGAACGGATCCTCTATTTGTGGCAGAAAATGCTGCTGATCTGGATTCAACTCTGGTTGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCACGGTCTGCCACCCGATACCCCAGCTGCTGCCGTTGAACGAGGGACAACGCCCCAACAAAGAACTGTTTTTGCAGAACTGAAGGACCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTAATCATCATTGGAAAAGTGGTTTCTCTCTCACCGCATTGGTCTCTTTCTTCCAAAGAAGCCTCCTGTTTGGTGGAGGCTTGA

Coding sequence (CDS)

ATGGCTCGCGTTTACGACCTCCAATCGCTTTCATCGCCGTTTTCGTCCCGCCCAAGAACACCCAGGACCCCAATTTTCAAACCCATCTCCTCAATCCACTGTTCCTCCTCTTCTTCCCCATTTACGGAGAAACATTCCATCAAGAGATACCAAAGAGACGATTGGGTGTACAAGAACCAAGCGGACCAAGCTTCAACTGCTCTGTCGTGTTCCGTTCCGTGCGATTCCGACTCAATACGGCAGGACGACATCGCTTTGCAGCTGCCGGAGCTGAAGAGGTTGCTGATGGTGCTGAGGGAGAAGAGGGTCAATGGTGGGTGCGATGATGGAGGAGAATGTGGGCCTGGGAATGTATTTCTAGTGGGGACTGGCCCTGGAGACCCTGAACTTTTGACATTGAAGGCTGTGAAAGTGATTCAGAGTGCCGATTTGCTTCTGTATGATCGTTTGGTGTCTAATGATGTGCTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTCGGCAAAACGGCAGGCTACCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTAGTGAGACTTAAAGGAGGAGATCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAAAAAGGGATTCAAGTAAAAATTGTTCCTGGTATAACTGCTGCTTCAGGTATAGCAGCTGAATTGGGGATTCCTTTAACGCACAGGGGTGTTGCAACGAACGTCAGGTTCCTCACTGGTCACTCTAGAAAGGGTGGAACGGATCCTCTATTTGTGGCAGAAAATGCTGCTGATCTGGATTCAACTCTGGTTGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCACGGTCTGCCACCCGATACCCCAGCTGCTGCCGTTGAACGAGGGACAACGCCCCAACAAAGAACTGTTTTTGCAGAACTGAAGGACCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTAATCATCATTGGAAAAGTGGTTTCTCTCTCACCGCATTGGTCTCTTTCTTCCAAAGAAGCCTCCTGTTTGGTGGAGGCTTGA

Protein sequence

MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASCLVEA
Homology
BLAST of Moc09g04970 vs. NCBI nr
Match: XP_022149015.1 (uncharacterized protein LOC111017535 [Momordica charantia])

HSP 1 Score: 728.4 bits (1879), Expect = 3.1e-206
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ
Sbjct: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120
           ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL
Sbjct: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEASCLVEA
Sbjct: 361 KEASCLVEA 369

BLAST of Moc09g04970 vs. NCBI nr
Match: XP_022991905.1 (uncharacterized protein LOC111488402 [Cucurbita maxima])

HSP 1 Score: 656.0 bits (1691), Expect = 1.9e-184
Identity = 335/369 (90.79%), Postives = 350/369 (94.85%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARV DLQSLSS FSSRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDWVYKNQ
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120
           AD++S A SCS+ CDS+SIRQ+DIALQLPELK+LL VLREKR + GCDD G+CGPGNVFL
Sbjct: 61  ADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDD-GKCGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAAD DSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEAS LVEA
Sbjct: 361 KEASSLVEA 368

BLAST of Moc09g04970 vs. NCBI nr
Match: XP_023548332.1 (uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652.9 bits (1683), Expect = 1.6e-183
Identity = 333/369 (90.24%), Postives = 350/369 (94.85%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARV DLQSLSS F+SRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDW+YKNQ
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120
           AD++S A SCS+P DS+SIRQ+DIALQLPELK+LL VLREKR + GCDD G+CGPGNVFL
Sbjct: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDD-GKCGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAAD DSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEAS LVEA
Sbjct: 361 KEASSLVEA 368

BLAST of Moc09g04970 vs. NCBI nr
Match: XP_008461254.1 (PREDICTED: uroporphyrinogen-III C-methyltransferase [Cucumis melo])

HSP 1 Score: 649.4 bits (1674), Expect = 1.8e-182
Identity = 333/373 (89.28%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHSIKRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPG 120
           YKNQ+DQ S   SCS+P DS+S+RQ+DIA+QLPELK+LL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPLFVAENAAD DSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

BLAST of Moc09g04970 vs. NCBI nr
Match: XP_004135909.1 (uncharacterized protein LOC101222061 [Cucumis sativus] >KGN45161.1 hypothetical protein Csa_016214 [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 4.5e-181
Identity = 330/373 (88.47%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHS+KRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSSASSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPG 120
           YK Q+DQ S   SCS+P DS+SIRQ+DIA+QLPELK+LL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKYQSDQPSVTSSCSIPYDSESIRQNDIAMQLPELKKLLEVLREKRVSNGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           +VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 DVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPL+VAENAAD DSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA+LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAQLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

BLAST of Moc09g04970 vs. ExPASy Swiss-Prot
Match: Q42606 (S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UPM1 PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 7.5e-147
Identity = 273/344 (79.36%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 28  PISSIH---CSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDI 87
           PI  +H    SSSSSPFTEKHS++RYQRD W+YK       +  S S   D   +R++DI
Sbjct: 26  PICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDI 85

Query: 88  ALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADL 147
           A QLPELK+LL VL+EKRV  GC  GG+CGPG+V+LVGTGPGDPELLTLKAV+VIQSADL
Sbjct: 86  ASQLPELKKLLAVLKEKRVK-GC-KGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADL 145

Query: 148 LLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 207
           LLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP
Sbjct: 146 LLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 205

Query: 208 LVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGG 267
           LVFGRGGEEMDFLQQ+GI+V+++PGITAASGIAAELGIPLTHRGVAT+VRFLTGHSRKGG
Sbjct: 206 LVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGG 265

Query: 268 TDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFA 327
           TDPLFVAENAAD D+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRTVFA
Sbjct: 266 TDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTVFA 325

Query: 328 ELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASCLVE 369
           ELKD A EI++A LVSPTLIIIGKVV LSP W   +KE+SCLVE
Sbjct: 326 ELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

BLAST of Moc09g04970 vs. ExPASy Swiss-Prot
Match: Q0VQ05 (Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=cysG PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.4e-60
Identity = 120/239 (50.21%), Postives = 161/239 (67.36%), Query Frame = 0

Query: 116 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYH 175
           G V+LVG GPGDP+LLT +A++++Q AD++LYDRLV   ++DL   DA L+YVGK    H
Sbjct: 218 GEVYLVGAGPGDPDLLTFRALRLLQKADVVLYDRLVGKGIVDLARRDAELVYVGKARDKH 277

Query: 176 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASG 235
           +  Q+ I+ELL+++A+ G  V RLKGGDP +FGRGGEE+D +  +GI  ++VPGITAASG
Sbjct: 278 ALPQDNINELLVHYAKQGKKVCRLKGGDPFIFGRGGEEIDLIVAEGIDFQVVPGITAASG 337

Query: 236 IAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLAL 295
            A+  GIPLTHR  A +VRF+TGH + G  D     ++      T+V YMGL  L  +  
Sbjct: 338 CASYAGIPLTHRDHAQSVRFVTGHRKDGSVD--LDWKHLVSETETVVFYMGLVGLREICS 397

Query: 296 KLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 355
           +L+ HG   DTP A V RGTT  Q  +   L  L D+I+  E+ +PTLII+G VVSL P
Sbjct: 398 QLIAHGRGGDTPIALVSRGTTNLQEVITGRLDQLPDDIEGREIHAPTLIIVGSVVSLHP 454

BLAST of Moc09g04970 vs. ExPASy Swiss-Prot
Match: Q6F8G6 (Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=cysG PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.8e-60
Identity = 124/237 (52.32%), Postives = 162/237 (68.35%), Query Frame = 0

Query: 116 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYH 175
           G V+LVG GPGDPELLTLKA++++Q AD+++YDRLVS  +L+L   DA  +YVGK    H
Sbjct: 216 GEVYLVGAGPGDPELLTLKALRLMQQADVVIYDRLVSAPILELCRRDAEKVYVGKARSNH 275

Query: 176 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASG 235
           S  QE I+ LL+ +A+AG  V RLKGGDP +FGRGGEE+  L   GI  ++VPGITAASG
Sbjct: 276 SVPQEGINALLVKYAQAGKRVCRLKGGDPFIFGRGGEEIQELFAAGIPFQVVPGITAASG 335

Query: 236 IAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLAL 295
            +A  GIPLTHR  A +VRFLTGH ++G   P          + TLV+YMGL  L  +  
Sbjct: 336 CSAYAGIPLTHRDYAQSVRFLTGHLKEG--SPELPWSELVYENQTLVLYMGLVGLEHICQ 395

Query: 296 KLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSL 353
           +L+ HG  PD P A V +GTTP+Q+ V   L ++A +I   ++ +PTL IIG+VVSL
Sbjct: 396 QLIAHGQRPDMPVALVSKGTTPEQKVVVGTLSNIASKIAEYQIHAPTLTIIGEVVSL 450

BLAST of Moc09g04970 vs. ExPASy Swiss-Prot
Match: Q21K21 (Siroheme synthase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) OX=203122 GN=cysG PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.3e-60
Identity = 119/251 (47.41%), Postives = 169/251 (67.33%), Query Frame = 0

Query: 100 EKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLV 159
           E+ +    D       G V+LVG GPGDP+LLT KA++++Q A+++LYDRLVS  +L++ 
Sbjct: 200 EQLIEAQLDSASVEKTGEVYLVGAGPGDPDLLTFKALRLMQQAEVVLYDRLVSEPILEMT 259

Query: 160 GPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ 219
             DA  +YVGK    H+  Q++I+++LL  A+ G  V+RLKGGDP +FGRGGEE+D L +
Sbjct: 260 RRDAERIYVGKKRAEHAVPQQKINQMLLELAQQGKRVLRLKGGDPFIFGRGGEEIDLLAE 319

Query: 220 KGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDS 279
             I  ++VPGITAASG A+  GIPLTHR  + +VRF+TGH ++G  +  F      D   
Sbjct: 320 HKIPFQVVPGITAASGCASYSGIPLTHRDYSQSVRFITGHLQEGKEN--FRWSEFVDKQQ 379

Query: 280 TLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELV 339
           TLV YMGL+ L ++  KL+ +G  P TPAA +ERGT P+QR   ++L  LA +I+  ++ 
Sbjct: 380 TLVFYMGLAGLETICSKLIEYGKSPSTPAALIERGTLPEQRVHVSDLAGLAAKIEGLDVH 439

Query: 340 SPTLIIIGKVV 351
           +PTL+IIG VV
Sbjct: 440 APTLLIIGDVV 448

BLAST of Moc09g04970 vs. ExPASy Swiss-Prot
Match: B8GUD3 (Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=cysG PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.3e-60
Identity = 126/255 (49.41%), Postives = 168/255 (65.88%), Query Frame = 0

Query: 100 EKRVNGGCD--DGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLD 159
           EK +  G D  D G    G VFLVG GPGDP+LLT +A++++Q AD+++YD LVS  +++
Sbjct: 204 EKALETGLDTRDAG----GEVFLVGAGPGDPDLLTFRALRLMQLADVVVYDNLVSPAIIE 263

Query: 160 LVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFL 219
           LV  DA ++Y GK    H+  QEEI++LL+  A+ G  V+RLKGGDP +FGRGGEE+D L
Sbjct: 264 LVRRDAEMIYAGKKRNLHTLPQEEINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIDTL 323

Query: 220 QQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADL 279
            Q+GI  ++VPGITAA+G A+  GIPLTHR  A  V F TGH R G  D  +  +  A  
Sbjct: 324 MQEGIPFQVVPGITAAAGCASFSGIPLTHRDYAQAVVFATGHLRDGSIDLNW--KMLAQP 383

Query: 280 DSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAE 339
             T+V YMGL  LP +  +LM HG+ PD P A VE+GTT  QR +   L  + D +K  +
Sbjct: 384 RQTVVFYMGLLGLPIICRELMAHGVSPDMPMALVEQGTTQNQRVIVGTLASMPDLVKDYD 443

Query: 340 LVSPTLIIIGKVVSL 353
           +  PTLII+G+VV L
Sbjct: 444 VQPPTLIIVGEVVKL 452

BLAST of Moc09g04970 vs. ExPASy TrEMBL
Match: A0A6J1D4K3 (uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017535 PE=3 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.5e-206
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ
Sbjct: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120
           ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL
Sbjct: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEASCLVEA
Sbjct: 361 KEASCLVEA 369

BLAST of Moc09g04970 vs. ExPASy TrEMBL
Match: A0A6J1JU84 (uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 9.4e-185
Identity = 335/369 (90.79%), Postives = 350/369 (94.85%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARV DLQSLSS FSSRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDWVYKNQ
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120
           AD++S A SCS+ CDS+SIRQ+DIALQLPELK+LL VLREKR + GCDD G+CGPGNVFL
Sbjct: 61  ADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDD-GKCGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAAD DSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEAS LVEA
Sbjct: 361 KEASSLVEA 368

BLAST of Moc09g04970 vs. ExPASy TrEMBL
Match: A0A1S3CDT8 (uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 8.8e-183
Identity = 333/373 (89.28%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHSIKRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPG 120
           YKNQ+DQ S   SCS+P DS+S+RQ+DIA+QLPELK+LL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPLFVAENAAD DSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

BLAST of Moc09g04970 vs. ExPASy TrEMBL
Match: A0A0A0KBI8 (TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428980 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.2e-181
Identity = 330/373 (88.47%), Postives = 349/373 (93.57%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHS+KRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSSASSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPG 120
           YK Q+DQ S   SCS+P DS+SIRQ+DIA+QLPELK+LL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKYQSDQPSVTSSCSIPYDSESIRQNDIAMQLPELKKLLEVLREKRVSNGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           +VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 DVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPL+VAENAAD DSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA+LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAQLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

BLAST of Moc09g04970 vs. ExPASy TrEMBL
Match: A0A6J1GQM1 (uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC111456604 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.4e-180
Identity = 332/376 (88.30%), Postives = 349/376 (92.82%), Query Frame = 0

Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC-------SSSSSPFTEKHSIKRYQRD 60
           MARV DLQSLSS F+SRP  PR+  FKPI S HC       SSSSSPFTEKHS+KRYQRD
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSSSSSSSSPFTEKHSVKRYQRD 60

Query: 61  DWVYKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGEC 120
           DW+YKNQAD++S A SCS+P DS+SIRQ+DIALQLPELK+LL VLREKR + G DD G+C
Sbjct: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGGDD-GKC 120

Query: 121 GPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAG 180
           GPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG
Sbjct: 121 GPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAG 180

Query: 181 YHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAA 240
           +HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAA
Sbjct: 181 FHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAA 240

Query: 241 SGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSL 300
           SGIAAELGIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAAD DSTLVVYMGLSTLPSL
Sbjct: 241 SGIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSL 300

Query: 301 ALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLS 360
           ALKLMHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLS
Sbjct: 301 ALKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLS 360

Query: 361 PHWSLSSKEASCLVEA 370
           PHWSLSSKEAS LVEA
Sbjct: 361 PHWSLSSKEASSLVEA 375

BLAST of Moc09g04970 vs. TAIR 10
Match: AT5G40850.1 (urophorphyrin methylase 1 )

HSP 1 Score: 521.5 bits (1342), Expect = 5.3e-148
Identity = 273/344 (79.36%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 28  PISSIH---CSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDI 87
           PI  +H    SSSSSPFTEKHS++RYQRD W+YK       +  S S   D   +R++DI
Sbjct: 26  PICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDI 85

Query: 88  ALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADL 147
           A QLPELK+LL VL+EKRV  GC  GG+CGPG+V+LVGTGPGDPELLTLKAV+VIQSADL
Sbjct: 86  ASQLPELKKLLAVLKEKRVK-GC-KGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADL 145

Query: 148 LLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 207
           LLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP
Sbjct: 146 LLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 205

Query: 208 LVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGG 267
           LVFGRGGEEMDFLQQ+GI+V+++PGITAASGIAAELGIPLTHRGVAT+VRFLTGHSRKGG
Sbjct: 206 LVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGG 265

Query: 268 TDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFA 327
           TDPLFVAENAAD D+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRTVFA
Sbjct: 266 TDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTVFA 325

Query: 328 ELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASCLVE 369
           ELKD A EI++A LVSPTLIIIGKVV LSP W   +KE+SCLVE
Sbjct: 326 ELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

BLAST of Moc09g04970 vs. TAIR 10
Match: AT5G40850.2 (urophorphyrin methylase 1 )

HSP 1 Score: 461.8 bits (1187), Expect = 5.0e-130
Identity = 242/314 (77.07%), Postives = 266/314 (84.71%), Query Frame = 0

Query: 28  PISSIH---CSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDI 87
           PI  +H    SSSSSPFTEKHS++RYQRD W+YK       +  S S   D   +R++DI
Sbjct: 26  PICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDI 85

Query: 88  ALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADL 147
           A QLPELK+LL VL+EKRV  GC  GG+CGPG+V+LVGTGPGDPELLTLKAV+VIQSADL
Sbjct: 86  ASQLPELKKLLAVLKEKRVK-GC-KGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADL 145

Query: 148 LLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 207
           LLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP
Sbjct: 146 LLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 205

Query: 208 LVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGG 267
           LVFGRGGEEMDFLQQ+GI+V+++PGITAASGIAAELGIPLTHRGVAT+VRFLTGHSRKGG
Sbjct: 206 LVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGG 265

Query: 268 TDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFA 327
           TDPLFVAENAAD D+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRT F 
Sbjct: 266 TDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTAFG 325

Query: 328 ELKDLADEIKAAEL 339
            L      +K  +L
Sbjct: 326 ILCRFLLSLKILQL 337

BLAST of Moc09g04970 vs. TAIR 10
Match: AT1G45110.1 (Tetrapyrrole (Corrin/Porphyrin) Methylases )

HSP 1 Score: 45.4 bits (106), Expect = 1.1e-04
Identity = 44/152 (28.95%), Postives = 69/152 (45.39%), Query Frame = 0

Query: 111 GECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLL-YDRLVSNDVLDLVGPDARLLYVG 170
           G   PG ++LVGT  G+ E +TL+A++V++SAD++L  D   S  +L      A+LL   
Sbjct: 51  GPLKPG-LYLVGTPIGNLEDITLRAIRVLRSADVILSEDTRHSGKLLQYYNIKAQLL--- 110

Query: 171 KTAGYHSRTQEEIHELLLNFAEAGATVVRLK-GGDPLVFGRGGEEMDFLQQKGIQVKIVP 230
               YH   + +  + +L   + G  V  +   G P +   G +      ++ I V  +P
Sbjct: 111 ---SYHKFNEAQREQAVLTRLKQGEIVALISDAGTPGISDPGTQLAKMCAKENIDVIPIP 170

Query: 231 GITAASGIAAELGIPLTHRGVATNVRFLTGHS 261
           G   A  + A L          T V FL  HS
Sbjct: 171 G---ACAVVAALSASGLETDEFTFVGFLPKHS 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149015.13.1e-206100.00uncharacterized protein LOC111017535 [Momordica charantia][more]
XP_022991905.11.9e-18490.79uncharacterized protein LOC111488402 [Cucurbita maxima][more]
XP_023548332.11.6e-18390.24uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo][more]
XP_008461254.11.8e-18289.28PREDICTED: uroporphyrinogen-III C-methyltransferase [Cucumis melo][more]
XP_004135909.14.5e-18188.47uncharacterized protein LOC101222061 [Cucumis sativus] >KGN45161.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q426067.5e-14779.36S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloro... [more]
Q0VQ051.4e-6050.21Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / N... [more]
Q6F8G61.8e-6052.32Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=... [more]
Q21K215.3e-6047.41Siroheme synthase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17... [more]
B8GUD35.3e-6049.41Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D4K31.5e-206100.00uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1JU849.4e-18590.79uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402... [more]
A0A1S3CDT88.8e-18389.28uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894... [more]
A0A0A0KBI82.2e-18188.47TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G42898... [more]
A0A6J1GQM12.4e-18088.30uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC1114566... [more]
Match NameE-valueIdentityDescription
AT5G40850.15.3e-14879.36urophorphyrin methylase 1 [more]
AT5G40850.25.0e-13077.07urophorphyrin methylase 1 [more]
AT1G45110.11.1e-0428.95Tetrapyrrole (Corrin/Porphyrin) Methylases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014777Tetrapyrrole methylase, subdomain 1GENE3D3.40.1010.10coord: 109..231
e-value: 1.0E-41
score: 144.0
IPR014776Tetrapyrrole methylase, subdomain 2GENE3D3.30.950.10coord: 232..367
e-value: 6.8E-44
score: 151.1
IPR006366Uroporphyrin-III C-methyltransferaseTIGRFAMTIGR01469TIGR01469coord: 116..352
e-value: 4.9E-87
score: 289.6
IPR006366Uroporphyrin-III C-methyltransferaseCDDcd11642SUMTcoord: 121..350
e-value: 1.96352E-123
score: 353.28
IPR000878Tetrapyrrole methylasePFAMPF00590TP_methylasecoord: 118..330
e-value: 1.1E-49
score: 169.2
NoneNo IPR availablePANTHERPTHR45790:SF3UROPORPHYRINOGEN-III C-METHYLTRANSFERASEcoord: 112..368
NoneNo IPR availablePANTHERPTHR45790SIROHEME SYNTHASE-RELATEDcoord: 112..368
IPR003043Uroporphiryn-III C-methyltransferase, conserved sitePROSITEPS00840SUMT_2coord: 196..229
IPR003043Uroporphiryn-III C-methyltransferase, conserved sitePROSITEPS00839SUMT_1coord: 121..135
IPR035996Tetrapyrrole methylase superfamilySUPERFAMILY53790Tetrapyrrole methylasecoord: 115..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g04970.1Moc09g04970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0019354 siroheme biosynthetic process
molecular_function GO:0004851 uroporphyrin-III C-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity