Moc09g03340 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc09g03340 vs. NCBI nr
Match: XP_022146255.1 (vacuolar protein sorting-associated protein IST1 [Momordica charantia])

HSP 1 Score: 850.9 bits (2197), Expect = 5.0e-243
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240
           YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300
           GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300

Query: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360
           RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY
Sbjct: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360

Query: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK 420
           SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK
Sbjct: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK 420

Query: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           RHTRNNSFVHPKLPDYDQLAARFAALKEK
Sbjct: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 449

BLAST of Moc09g03340 vs. NCBI nr
Match: XP_038897244.1 (uncharacterized protein LOC120085370 [Benincasa hispida])

HSP 1 Score: 579.7 bits (1493), Expect = 2.2e-161
Identity = 328/472 (69.49%), Postives = 379/472 (80.30%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMF KPKF+TKCKSCVK+ K RLD+IRKKK AVLK+LKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDSIRKKKKAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVERNVL+CY LIDEF GTI +Q+ VLSK+SECP+EC+EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYLLIDEFCGTISSQIPVLSKESECPEECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELRSLFT KYG SF  FTNKELIEK  A A TKE+K+QLLQEIAQ+S I+WNSKALEQQL
Sbjct: 121 ELRSLFTEKYGSSFGSFTNKELIEKSRATAQTKEMKIQLLQEIAQDSAIDWNSKALEQQL 180

Query: 181 YT-PPQNTPDEEPNAD-RRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTET 240
           YT PPQN P  E +A  +RNK K+V++PV ERKT   +KKDNSD++SIFDSRSE N TET
Sbjct: 181 YTPPPQNEPAGERSATIKRNKTKIVTVPVHERKTISPRKKDNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE------------ 300
           STGD STDQDV KG+ EDEVED+KPFYLRF+PPPYLK+KP K EA+ E            
Sbjct: 241 STGD-STDQDVHKGISEDEVEDQKPFYLRFVPPPYLKTKPIKTEANVEESQKVTVGNNDE 300

Query: 301 ------KPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMM 360
                 K   EEKPKPRSVRRRN K +P RDINI +  +S  DG EK S S+NKGKETM+
Sbjct: 301 SNKQHPKSPTEEKPKPRSVRRRNVKPQPARDINIDDAGSSTIDGAEKTSLSQNKGKETMI 360

Query: 361 GEEKGAG-EEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQD--EPQRTKNSKAEE 420
           GEE+GAG +EER+LDGLLM YS+KK++QESNR KGNLK Q+ +E+D  EPQ+ K++K  E
Sbjct: 361 GEERGAGDDEERVLDGLLMQYSRKKTSQESNRGKGNLKPQRQQEKDNAEPQKPKSTKT-E 420

Query: 421 DLSVPGRSVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           D  VP R+VSLP D +EP+   K+HTR NSFVHPKLP+YDQLAAR AALKEK
Sbjct: 421 DSFVPARAVSLPTDQSEPV---KKHTRTNSFVHPKLPEYDQLAARIAALKEK 467

BLAST of Moc09g03340 vs. NCBI nr
Match: KAG6575541.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562.4 bits (1448), Expect = 3.6e-156
Identity = 317/465 (68.17%), Postives = 360/465 (77.42%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNKEL+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E   +  RNK+K+VS+PV+ R T+  +KK+NSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDRERSTSTNRNKSKIVSVPVYGRTTNSPRKKENSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SS RNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++  ED  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRT-EDAYVPTK 420

Query: 421 SVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           S+SLP    EPI P+K+H R NS+VHPKLPDYDQLAARFAALKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Moc09g03340 vs. NCBI nr
Match: KAG7014085.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 562.0 bits (1447), Expect = 4.6e-156
Identity = 317/465 (68.17%), Postives = 360/465 (77.42%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNKEL+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SS RNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++  ED  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRT-EDAYVPTK 420

Query: 421 SVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           S+SLP    EPI P+K+H R NS+VHPKLPDYDQLAARFAALKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Moc09g03340 vs. NCBI nr
Match: XP_022953212.1 (uncharacterized protein LOC111455825 [Cucurbita moschata])

HSP 1 Score: 560.8 bits (1444), Expect = 1.0e-155
Identity = 317/465 (68.17%), Postives = 361/465 (77.63%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNKEL+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SSSRNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++  ED  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRT-EDAYVPTK 420

Query: 421 SVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           S+SLP    EPI P+K+H R NS+VHPKLPDYDQLAARFA+LKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Moc09g03340 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.6e-13
Identity = 42/167 (25.15%), Postives = 93/167 (55.69%), Query Frame = 0

Query: 13  KCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVLKCY 72
           K K  +KL   R+  ++ KK  +++  K ++AELLR   +++A  R E ++ +  +++C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  DLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELRELRSLFTAKYGR 132
            +I+     ++ ++++++  +E P E +E++ +L+Y++ R   +PEL ++++   AKYG+
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 SFEQ--------FTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEW 172
             E           N +++ K S       +  Q L EIA++  ++W
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of Moc09g03340 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 4.6e-05
Identity = 38/163 (23.31%), Postives = 81/163 (49.69%), Query Frame = 0

Query: 18  VKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVLKCYDLIDE 77
           ++L+  RL  + KKK  + +  + +IA+ L +G D+ A  R E ++ E  +++  ++++ 
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 78  FSGTIYNQLSVLSKQSECPDECREAVASLIYAAARF-ADLPELRELRSLFTAKYGRSFEQ 137
           +   +  +  ++    E      E+V++LI+AA R  +++ EL+ +     AKY + + +
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGK 134

Query: 138 F--------TNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEW 172
                     N  L+ K S  A  K +  + L EIA+   + +
Sbjct: 135 LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPY 177

BLAST of Moc09g03340 vs. ExPASy Swiss-Prot
Match: P53843 (Vacuolar protein sorting-associated protein IST1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=IST1 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 8.6e-04
Identity = 59/270 (21.85%), Postives = 119/270 (44.07%), Query Frame = 0

Query: 10  FFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVL 69
           F  K K+C+K+   RL   ++K+ A+ K  +  +A+LL +  +Q A+ R E L+ +   +
Sbjct: 8   FTIKLKTCLKMCIQRLRYAQEKQQAIAKQSRRQVAQLLLTNKEQKAHYRVETLIHDDIHI 67

Query: 70  KCYDLIDEFSGTIYNQLSVLSK--------QSECPDECREAVASLIYAAARFADLPELRE 129
           +  ++++ +   +  ++ V++         +    D   EA+ SLIYA     ++ EL +
Sbjct: 68  ELLEILELYCELLLARVQVINDISTEEQLVKEHMDDGINEAIRSLIYAILFVDEVKELSQ 127

Query: 130 LRSLFTAKYGRSF------EQFTNKELIEKPSAMAMTKEVKLQL-LQEIAQESGIEWNSK 189
           L+ L   K    F      +     E I K  + ++ KE  + L L+EIA+   + ++  
Sbjct: 128 LKDLMAWKINVEFVNGVIADHIDVPEKIIKKCSPSVPKEELVDLYLKEIAKTYDVPYSK- 187

Query: 190 ALEQQLYTPPQNTPDE--EPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSE 249
            LE  L +   N   +  +P+ D  +  +   I   +         DN+D       +  
Sbjct: 188 -LENSLSSSSSNISSDFSDPSGDIEDNDEEKPILALDN-----DDNDNADAKHPITVKKP 247

Query: 250 DNGTETSTGDSSTDQDVQKGVFEDEVEDRK 263
              +E    +    +D++K V E +  ++K
Sbjct: 248 RQNSENIKNELKIPKDIKKEVIEKKQSEKK 270

BLAST of Moc09g03340 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.4e-243
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240
           YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300
           GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300

Query: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360
           RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY
Sbjct: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360

Query: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK 420
           SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK
Sbjct: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK 420

Query: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           RHTRNNSFVHPKLPDYDQLAARFAALKEK
Sbjct: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 449

BLAST of Moc09g03340 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 5.0e-156
Identity = 317/465 (68.17%), Postives = 361/465 (77.63%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNKEL+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SSSRNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++  ED  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRT-EDAYVPTK 420

Query: 421 SVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           S+SLP    EPI P+K+H R NS+VHPKLPDYDQLAARFA+LKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Moc09g03340 vs. ExPASy TrEMBL
Match: A0A6J1JV69 (uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 4.7e-154
Identity = 314/465 (67.53%), Postives = 357/465 (76.77%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+N +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNKEL+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQN-TPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN    E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELVRERSTSTNRSKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKG-VFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KG V EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGIVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEKRNN 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
                EEKPKPRSVRRR++K +P R INI +   SA DG  K SS +NKGKE M GEEKG
Sbjct: 301 PKTTTEEKPKPRSVRRRHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAMTGEEKG 360

Query: 361 -AGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKK + QE             K+  EPQR KN++  ED  VP +
Sbjct: 361 EEDDEERMLDGLLKIYSKKNTRQED------------KDNAEPQRPKNTRT-EDAYVPAK 420

Query: 421 SVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
           S+SLP    EPI P+K+H R NS+VHPKLPDYDQLAARFAALKEK
Sbjct: 421 SLSLPTARTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Moc09g03340 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 8.3e-151
Identity = 314/468 (67.09%), Postives = 363/468 (77.56%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMF KPKF+TKCKSCVK+ K RLDTIRKKKNAVLK+LKNDI ELL+S LD NA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQ+ VL+K+SECPDEC+EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELR+LFT KYG SF  FTNKE IEK  A   TKE+K+QLLQEIAQE+ I+WNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YT-PPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTET 240
           YT PPQN  D E   A +RNK KVVS+PV+ERK +  + K+NSD++SIFDSRSE N TET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
           STGD STDQD+ KGV EDEVED+KPF  RF+ PPYLK+KP K EA+ ++P          
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDES 300

Query: 301 -----AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEE 360
                  EEKPKPRSVRRRN K +P RDINI +V +S  D T K SSSRNKGKETM+GEE
Sbjct: 301 NNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEE 360

Query: 361 KGA-GEEERMLDGLLMHYSKKKSTQES-NRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSV 420
           KGA  +EER+LDGLLM YSKKK+ QES +R K NLK Q+ +E+D  +  + +        
Sbjct: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTT------- 420

Query: 421 PGRSVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
             R+VSLP D NE     K+HTR NSFVHPKLP+YDQLAAR AALKEK
Sbjct: 421 --RAVSLPTDQNE---STKKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of Moc09g03340 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.2e-146
Identity = 309/468 (66.03%), Postives = 358/468 (76.50%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMF KPKF+TKCKSCVK+ K RLDT RKKKNAVLK+LKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQ+ VL+K+SECPDEC+E+VA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELR+LFT KYG SF  FTNKE IEK      TKE+K+QLLQEIAQE+ I+WNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YT-PPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTET 240
           YT PP+N  D E   A +RNK KVVS+PV+E+K +  + K+NSD++SIFDSRSE N TET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE------------ 300
           STGD STDQDV KGV  DEV D+KPF  RF+ PPYLK+KP K EA+ E            
Sbjct: 241 STGD-STDQDVHKGVSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDE 300

Query: 301 ---KPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEE 360
              K   EEKPKPRSVRRR  K +P RDINI +V +S  D T+K SS RNKGKETM+GEE
Sbjct: 301 SNPKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGEE 360

Query: 361 KGA-GEEERMLDGLLMHYSKKKSTQES-NRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSV 420
           KGA  +EER+LDGLLM YSKKK+ QES +R K NLK Q+ +E+D  +  + +        
Sbjct: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT-------- 420

Query: 421 PGRSVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 450
             R+VS P D NE   PMK+HTR NSFVHPKLP+YDQLAAR AALKEK
Sbjct: 421 -SRAVSFPPDQNE---PMKKHTRTNSFVHPKLPEYDQLAARIAALKEK 454

BLAST of Moc09g03340 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 243.0 bits (619), Expect = 4.5e-64
Identity = 150/326 (46.01%), Postives = 211/326 (64.72%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFD  FKPKF+TKCKS VK+ K R+DT+++KKN+V K+LKNDI +LL++ LD NAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GL+ E+  L CY+ +++F   + + +S+L K   CPDECREA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           +LRSLF  +YG + +QF N E +E+  A   +KE+K++LLQEIA+E  I+W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YTPPQ----NTPDEEPNADRRNKAKVVSIPVFERKTSL-LQKKDNSDDDS--IFDSRSED 240
           YTPP     +T  EEP  ++ N     ++   E+++SL    +++S DDS     S SE+
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTM---EKRSSLSFHGREDSMDDSNKSIMSTSEE 240

Query: 241 NGTETSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFI-PPPYLKSKPSKKEASFEKPAVEE 300
           +   TS G   T         ED+ E  KPFY RF+ P PY K K  K+E+  EK     
Sbjct: 241 DSMSTS-GSCMTGP-------EDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSH 300

Query: 301 ----------KPKPRSVRRRNSKLEP 309
                     KPKPRSVRRR +   P
Sbjct: 301 LMDTESPTGGKPKPRSVRRRFANPPP 314

BLAST of Moc09g03340 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-40
Identity = 112/267 (41.95%), Postives = 162/267 (60.67%), Query Frame = 0

Query: 60  EGLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPEL 119
           EGL+ E+  L CY+ +++F   + + +S+L K   CPDECREA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQ 179
           R+LRSLF  +YG + +QF N E +E+  A   +KE+K++LLQEIA+E  I+W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYTPPQ----NTPDEEPNADRRNKAKVVSIPVFERKTSL-LQKKDNSDDDS--IFDSRSE 239
           LYTPP     +T  EEP  ++ N     ++   E+++SL    +++S DDS     S SE
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTM---EKRSSLSFHGREDSMDDSNKSIMSTSE 225

Query: 240 DNGTETSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFI-PPPYLKSKPSKKEASFEKPAVE 299
           ++   TS G   T         ED+ E  KPFY RF+ P PY K K  K+E+  EK    
Sbjct: 226 EDSMSTS-GSCMTGP-------EDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTS 285

Query: 300 E----------KPKPRSVRRRNSKLEP 309
                      KPKPRSVRRR +   P
Sbjct: 286 HLMDTESPTGGKPKPRSVRRRFANPPP 300

BLAST of Moc09g03340 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 147.9 bits (372), Expect = 1.9e-35
Identity = 111/317 (35.02%), Postives = 175/317 (55.21%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF   FKPKF+ K KS    +K+R+D +R+K+ A+++  K DI   L++G D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
            LL E  ++ CYDLI+ F   I   LS++ K+ ECP+ECREAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAM-AMTKEVKLQLLQEIAQESGIEWNSKALEQQ 180
           +LR++FT ++G       N EL+EK   +   ++E+K+Q ++++A E  I W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LY--TPPQNTPDE-EPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSI------FDSR 240
           L   T      D+ E  AD  NK         + + S +   ++SDD+S+       DS 
Sbjct: 181 LLRETSALQIKDKVETRADYLNK---------DTEKSFMD--EHSDDESVLSESWRIDSL 240

Query: 241 SEDNGTETSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAV 300
           S+ + + +S+  SS+ +          V  RK    + +P   + S  +K  A  ++ A 
Sbjct: 241 SKGSLSSSSSSSSSSLR---------RVSVRKKKGKKILPYGIISSPDTKLGAGNDEKAQ 297

Query: 301 EEKPKPRSVR-RRNSKL 307
           EEK + R +  + NS+L
Sbjct: 301 EEKEEKRKIADQENSRL 297

BLAST of Moc09g03340 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 130.2 bits (326), Expect = 4.2e-30
Identity = 137/466 (29.40%), Postives = 212/466 (45.49%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFD F    F  K K  +KL K R+D +R+K+NA +KFLK D+A+L+ +G D NA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GLL E   L   D +++    +Y QLS + K  ECP++CREA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELR +F  KY  S   F N+EL+E  S+   + E K++L++++A E  I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 --------YTPPQNTPDEEPNADRRNK-AKVVSIPVFERKTSLLQKKDNSDD--DSIF-- 240
                      P++T D+    DR     K       E   SL +K   + +  D +F  
Sbjct: 181 VRQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLFQS 240

Query: 241 DSRSEDNGTE------TSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKK 300
           D  S  NG        T    S       +   +D   +RK FYL      + K  P++ 
Sbjct: 241 DKESYQNGLRGNQRGLTYKERSENVLHASRSESKDNKAERKEFYL------HSKQNPAR- 300

Query: 301 EASFEKPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDN---------SANDGTEKASSSR 360
                     EK +P      N        +N GN+            A+  TE  +S R
Sbjct: 301 ----------EKHQPIF----NEGDTIVMKVNYGNLGQGNGHRPGVVDAHKKTEFVASER 360

Query: 361 NKGKETMMGEEKGAGEEERMLDG--LLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRT 420
            +       E      +    DG  ++M    +   Q +    G +   K  E +  ++ 
Sbjct: 361 KEFYSQSKQEPSRERHQPIFNDGDTIVMKVKHENHVQGNGHKNGVVDLHKKIEVNASEKL 420

Query: 421 KNSKAEE-DLSVPGRSVSLPRDTNEPIVPMKRHTRNNSFVHPKLPD 436
           K+S ++  D  V G          E      +H +N    H K+ D
Sbjct: 421 KSSSSKRADKLVIGFK-------QESFFQGYKHEKNEEHAHQKVED 438

BLAST of Moc09g03340 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 115.9 bits (289), Expect = 8.2e-26
Identity = 131/484 (27.07%), Postives = 215/484 (44.42%), Query Frame = 0

Query: 12  TKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVLKC 71
           +KCK+  K+   R+  IR K+  V+K ++ DIA LL+SG D  A  R E ++ E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELRELRSLFTAKYG 131
            ++I+ F   I ++L++++KQ +CP + +E +ASLI+AA R +++PEL +LR +F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 RSFEQF---------TNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQLYT 191
           + F             N+ LI+K S      E KL++++EIA+E  ++W++   EQ+L  
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELLK 208

Query: 192 PPQNTPDEEPNADRRNKAKVVSIPV--------------FERKTSLLQKKDNSDDD---- 251
           P + + D       R      S+PV                R TS +    +  D     
Sbjct: 209 PQEESID-----GPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAA 268

Query: 252 -----------------SIFDSRSEDNGTETS-TGDSSTDQDVQKGVFEDEVEDRKPFYL 311
                            S+  +R + +  E S + D ST Q       + +  D    + 
Sbjct: 269 EAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQK------DSQYMDHHHHH- 328

Query: 312 RFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTE 371
              P    +S+ S+  + + KP  E     R + RR+S           N   + +D  E
Sbjct: 329 ---PGSRRQSRDSETSSYYAKPGAEN----RGMGRRHS---------YNNPGINESDYEE 388

Query: 372 KASSSRNKGKETMMGEEKGAGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDE 431
           + +++  + KETM                    Y+ +     +       +S  ++E+ E
Sbjct: 389 EYTNTEAEAKETMRRRHS---------------YNPRSVPPPATSEIKFDESDYYEEETE 448

Query: 432 PQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMKRHTRNNSF---VHPKLPDYDQLAARF 448
           P          D    GR  SLP +   P  P    +R +S    VHPKLPDYD LAARF
Sbjct: 449 P----------DEPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQVHPKLPDYDILAARF 459