Moc09g02010 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g02010
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionnodulation-signaling pathway 1 protein
Locationchr9: 1656410 .. 1658044 (-)
RNA-Seq ExpressionMoc09g02010
SyntenyMoc09g02010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCATTGAAGAACCAGGGCCAAACCACCCTTCAGATCACATATTGGACTGGTTAGAGGATTCAGCTCCTTTCTTTTCCCCATTCCTGGACGAGACTTACAACTCTAGCTCTATAAACTGCTATCAATGGTGGGATGAGAGCCAAAACATAGGCCAAGATCTGATTAATGGCTGTCTCAGTAGCTCCCCTGCCGCTGCCACCACAGACAGTACTACACCACCAAACACTACCAGTTTGACGCCATCGGATTTGTCAAAGAAAAGGAAAGCCCCAGATGACACAGGTCATAAGACAACACAACCCCATCAGAACCCAAGGAAGAACCAGAACAATCAGAGCAAAAATGGTGCAGATAAAGGCGGTGGGGGGGTGGTAAAGAAGTCAGTGGGGAACAAGAAAAGTTCATCAAAATCCACAGGAAATAATTATAATAACGGAAGTAACAAGGAAGGAAGGTGGGCGGAGCAATTGCTAAATCCCTGTGCAAATGCTATCATAAAAGGAGATGCGACACGAGTACATCACCTTATTTGTGTTCTGCAAGAGCTCGCTTCCCCCACCGGCGACGCCAATCACCGGCTCGCCGATCATGGTCTCCGAGCTCTGGCCCATCACCTGTCCTCCAATTCATCATCTTCTTCTTCCACACTTGCGCCGGTGGTTAATTTCGCTTCGACGGACGCGCGATTCTTCCAGCGGTCGTTGATCAAATTCCACGAGGTGAGTCCCTGGTTTGCTCTTCCGAACAACATCGCGAATTCTTCAATCCTCCACACTCTCTCTGAAGAACCTAATCTCTCGCGCAATCTTCACATTCTTGACATTGGGGTTTCTCACGGTGTGCAATGGCCGACGCTGCTCGAGGCCTTGACTCGCCGTTCCGGTGGGCCTCCGCCGCTAATTCGGCTCACAGTTGTCGCTCCCACCGTCGAACACGACCAAACTGCGGAGACGCCGTTCTCCATTGGTCCACCGGGAGACAACATCTCCTCTCGGCTACTTAGTTTCGCCAAATCCTTGAACATCAATTTACAGATCAACCGCCTCGACAATCACTCGTTACAGAACTTAAATTCGCAAGTAATCGGTAAGTTCCGGGACGAAATTTTGATCGTTTGCGCACACTTCAGACTCCACCAGTTGAAACACCGCGCTCCAGACGAAAGAACAGAGTTCCTACGAAATCTGAGAAAAATGGAGCCAAATGCAGTGATTCTGAGCGAAAACAACATAGCATGTAGCTGCAGCAACTGCGGGAATTTCGACATCACATTCACTCGGCGAGTGGAGTACTTGTGGAGGTTTCTGGACTCGACGAGCTCCGCATTCAAAGGGCGAGAAAGCGACGAAAGAAGAGTGATGGAAGGAGAGGCGGCGAAGGCGTTGACGAATCAGGGCGAAATGAACGAGGAAAAGGAGAAATGGTACGAGAGAATGAGAAATGCAGGATTCGCTAGAAAATTCTTCGCAGAAGGCACCATTGATACGGCTCGAGCTTCCATGAGAAGATATGACAATAACTGGGAAATGAGAATGGAAGAGAAAGATGGATGCGTGGGGTTATGGTGGAAAGGGCAGCCAATTTCGTTTTGTTCGTTTTGGAAATTGGGAGCGAAACCCAATGGCGGTTAA

mRNA sequence

ATGACCATTGAAGAACCAGGGCCAAACCACCCTTCAGATCACATATTGGACTGGTTAGAGGATTCAGCTCCTTTCTTTTCCCCATTCCTGGACGAGACTTACAACTCTAGCTCTATAAACTGCTATCAATGGTGGGATGAGAGCCAAAACATAGGCCAAGATCTGATTAATGGCTGTCTCAGTAGCTCCCCTGCCGCTGCCACCACAGACAGTACTACACCACCAAACACTACCAGTTTGACGCCATCGGATTTGTCAAAGAAAAGGAAAGCCCCAGATGACACAGGTCATAAGACAACACAACCCCATCAGAACCCAAGGAAGAACCAGAACAATCAGAGCAAAAATGGTGCAGATAAAGGCGGTGGGGGGGTGGTAAAGAAGTCAGTGGGGAACAAGAAAAGTTCATCAAAATCCACAGGAAATAATTATAATAACGGAAGTAACAAGGAAGGAAGGTGGGCGGAGCAATTGCTAAATCCCTGTGCAAATGCTATCATAAAAGGAGATGCGACACGAGTACATCACCTTATTTGTGTTCTGCAAGAGCTCGCTTCCCCCACCGGCGACGCCAATCACCGGCTCGCCGATCATGGTCTCCGAGCTCTGGCCCATCACCTGTCCTCCAATTCATCATCTTCTTCTTCCACACTTGCGCCGGTGGTTAATTTCGCTTCGACGGACGCGCGATTCTTCCAGCGGTCGTTGATCAAATTCCACGAGGTGAGTCCCTGGTTTGCTCTTCCGAACAACATCGCGAATTCTTCAATCCTCCACACTCTCTCTGAAGAACCTAATCTCTCGCGCAATCTTCACATTCTTGACATTGGGGTTTCTCACGGTGTGCAATGGCCGACGCTGCTCGAGGCCTTGACTCGCCGTTCCGGTGGGCCTCCGCCGCTAATTCGGCTCACAGTTGTCGCTCCCACCGTCGAACACGACCAAACTGCGGAGACGCCGTTCTCCATTGGTCCACCGGGAGACAACATCTCCTCTCGGCTACTTAGTTTCGCCAAATCCTTGAACATCAATTTACAGATCAACCGCCTCGACAATCACTCGTTACAGAACTTAAATTCGCAAGTAATCGGTAAGTTCCGGGACGAAATTTTGATCGTTTGCGCACACTTCAGACTCCACCAGTTGAAACACCGCGCTCCAGACGAAAGAACAGAGTTCCTACGAAATCTGAGAAAAATGGAGCCAAATGCAGTGATTCTGAGCGAAAACAACATAGCATGTAGCTGCAGCAACTGCGGGAATTTCGACATCACATTCACTCGGCGAGTGGAGTACTTGTGGAGGTTTCTGGACTCGACGAGCTCCGCATTCAAAGGGCGAGAAAGCGACGAAAGAAGAGTGATGGAAGGAGAGGCGGCGAAGGCGTTGACGAATCAGGGCGAAATGAACGAGGAAAAGGAGAAATGGTACGAGAGAATGAGAAATGCAGGATTCGCTAGAAAATTCTTCGCAGAAGGCACCATTGATACGGCTCGAGCTTCCATGAGAAGATATGACAATAACTGGGAAATGAGAATGGAAGAGAAAGATGGATGCGTGGGGTTATGGTGGAAAGGGCAGCCAATTTCGTTTTGTTCGTTTTGGAAATTGGGAGCGAAACCCAATGGCGGTTAA

Coding sequence (CDS)

ATGACCATTGAAGAACCAGGGCCAAACCACCCTTCAGATCACATATTGGACTGGTTAGAGGATTCAGCTCCTTTCTTTTCCCCATTCCTGGACGAGACTTACAACTCTAGCTCTATAAACTGCTATCAATGGTGGGATGAGAGCCAAAACATAGGCCAAGATCTGATTAATGGCTGTCTCAGTAGCTCCCCTGCCGCTGCCACCACAGACAGTACTACACCACCAAACACTACCAGTTTGACGCCATCGGATTTGTCAAAGAAAAGGAAAGCCCCAGATGACACAGGTCATAAGACAACACAACCCCATCAGAACCCAAGGAAGAACCAGAACAATCAGAGCAAAAATGGTGCAGATAAAGGCGGTGGGGGGGTGGTAAAGAAGTCAGTGGGGAACAAGAAAAGTTCATCAAAATCCACAGGAAATAATTATAATAACGGAAGTAACAAGGAAGGAAGGTGGGCGGAGCAATTGCTAAATCCCTGTGCAAATGCTATCATAAAAGGAGATGCGACACGAGTACATCACCTTATTTGTGTTCTGCAAGAGCTCGCTTCCCCCACCGGCGACGCCAATCACCGGCTCGCCGATCATGGTCTCCGAGCTCTGGCCCATCACCTGTCCTCCAATTCATCATCTTCTTCTTCCACACTTGCGCCGGTGGTTAATTTCGCTTCGACGGACGCGCGATTCTTCCAGCGGTCGTTGATCAAATTCCACGAGGTGAGTCCCTGGTTTGCTCTTCCGAACAACATCGCGAATTCTTCAATCCTCCACACTCTCTCTGAAGAACCTAATCTCTCGCGCAATCTTCACATTCTTGACATTGGGGTTTCTCACGGTGTGCAATGGCCGACGCTGCTCGAGGCCTTGACTCGCCGTTCCGGTGGGCCTCCGCCGCTAATTCGGCTCACAGTTGTCGCTCCCACCGTCGAACACGACCAAACTGCGGAGACGCCGTTCTCCATTGGTCCACCGGGAGACAACATCTCCTCTCGGCTACTTAGTTTCGCCAAATCCTTGAACATCAATTTACAGATCAACCGCCTCGACAATCACTCGTTACAGAACTTAAATTCGCAAGTAATCGGTAAGTTCCGGGACGAAATTTTGATCGTTTGCGCACACTTCAGACTCCACCAGTTGAAACACCGCGCTCCAGACGAAAGAACAGAGTTCCTACGAAATCTGAGAAAAATGGAGCCAAATGCAGTGATTCTGAGCGAAAACAACATAGCATGTAGCTGCAGCAACTGCGGGAATTTCGACATCACATTCACTCGGCGAGTGGAGTACTTGTGGAGGTTTCTGGACTCGACGAGCTCCGCATTCAAAGGGCGAGAAAGCGACGAAAGAAGAGTGATGGAAGGAGAGGCGGCGAAGGCGTTGACGAATCAGGGCGAAATGAACGAGGAAAAGGAGAAATGGTACGAGAGAATGAGAAATGCAGGATTCGCTAGAAAATTCTTCGCAGAAGGCACCATTGATACGGCTCGAGCTTCCATGAGAAGATATGACAATAACTGGGAAATGAGAATGGAAGAGAAAGATGGATGCGTGGGGTTATGGTGGAAAGGGCAGCCAATTTCGTTTTGTTCGTTTTGGAAATTGGGAGCGAAACCCAATGGCGGTTAA

Protein sequence

MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCLSSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADKGGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAKPNGG
Homology
BLAST of Moc09g02010 vs. NCBI nr
Match: XP_022148342.1 (nodulation-signaling pathway 1 protein [Momordica charantia])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 543/544 (99.82%), Postives = 543/544 (99.82%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60
           MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL
Sbjct: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60

Query: 61  SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK 120
           SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK
Sbjct: 61  SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK 120

Query: 121 GGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICV 180
           GGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICV
Sbjct: 121 GGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICV 180

Query: 181 LQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFH 240
           LQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFH
Sbjct: 181 LQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFH 240

Query: 241 EVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPP 300
           EVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPP
Sbjct: 241 EVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPP 300

Query: 301 LIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNS 360
           LIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNS
Sbjct: 301 LIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNS 360

Query: 361 QVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCG 420
           QVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCG
Sbjct: 361 QVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCG 420

Query: 421 NFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERM 480
           NFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERM
Sbjct: 421 NFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERM 480

Query: 481 RNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAK 540
           RNAGFARKFFAE TIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAK
Sbjct: 481 RNAGFARKFFAEXTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAK 540

Query: 541 PNGG 545
           PNGG
Sbjct: 541 PNGG 544

BLAST of Moc09g02010 vs. NCBI nr
Match: XP_023000031.1 (nodulation-signaling pathway 1 protein [Cucurbita maxima])

HSP 1 Score: 922.2 bits (2382), Expect = 2.1e-264
Identity = 472/555 (85.05%), Postives = 501/555 (90.27%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFF-SPFLDETYNSSSINCYQWWDESQNIGQDLINGC 60
           MTIEEPG NHPSDHILDWL DS PFF SPF D++YNSSSINCYQWWDE+Q+IGQDLINGC
Sbjct: 40  MTIEEPGANHPSDHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGC 99

Query: 61  LSSSPAAATTDSTTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKN 120
           LSSSP   TT ST PPNT++   LTPSDL+KKRKAPDDT HK +Q  QN RKNQNNQSKN
Sbjct: 100 LSSSP---TTVSTKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKN 159

Query: 121 GADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDA 180
           GADKG G      V+KKSVGNK++SSK+TGNN +NGSNKEGRWAEQLLNPCANAIIKGDA
Sbjct: 160 GADKGSGAVEGVTVIKKSVGNKRNSSKATGNNNHNGSNKEGRWAEQLLNPCANAIIKGDA 219

Query: 181 TRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSS--SSSSTLAPVVNFASTDA 240
           TRVHHL+CVLQELASPTGDANHRLA +GLRALAH+LSSNSS  SSSST+AP V FASTD 
Sbjct: 220 TRVHHLLCVLQELASPTGDANHRLAAYGLRALAHYLSSNSSCYSSSSTVAP-VTFASTDL 279

Query: 241 RFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFA PNNIANSSILH LSEEPN SRNLHILDIGVSHGVQWPTLLE
Sbjct: 280 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLE 339

Query: 301 ALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTV+APTVEHDQ AETPFSIGPPGDNISSRLLSFAKSLNINLQINR
Sbjct: 340 ALTRRSGGPPPLIRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 399

Query: 361 LDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSE 420
           LDN SLQ+ NSQVIGK  DEILIVCA FRLHQLKH APDER EFL+NLRK+EP AVILSE
Sbjct: 400 LDNRSLQSFNSQVIGKLPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSE 459

Query: 421 NNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480
           NN+ACSC+NCGNFD  FTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAA+ LTNQGEM
Sbjct: 460 NNMACSCNNCGNFDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAARGLTNQGEM 519

Query: 481 NEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPI 540
           NEE EKW ERMRNAGFARK F E TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+
Sbjct: 520 NEEMEKWCERMRNAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPV 579

Query: 541 SFCSFWKLGAKPNGG 545
           SFCSFWKLG K NGG
Sbjct: 580 SFCSFWKLGMKSNGG 590

BLAST of Moc09g02010 vs. NCBI nr
Match: XP_038897214.1 (protein NODULATION SIGNALING PATHWAY 1 [Benincasa hispida])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 460/552 (83.33%), Postives = 486/552 (88.04%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDS PFFSPFLDET NSSSINCYQWWD +Q+ G+DLING L
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSPFLDETNNSSSINCYQWWDANQDTGEDLINGFL 60

Query: 61  SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK 120
           S+SP   +T     P +  LTPSDL+KKRKAPDD+ HK +Q HQN RKNQNNQSKNG   
Sbjct: 61  SNSPTTVSTKLQNIPTSHHLTPSDLTKKRKAPDDSVHKKSQTHQNRRKNQNNQSKNG--- 120

Query: 121 GGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVH 180
           GGG      V+KKSVGNKK+SSK TGNN NNGSN+EGRWAEQLLNPCA+AIIKGDATRVH
Sbjct: 121 GGGAVEGVTVMKKSVGNKKNSSKFTGNNCNNGSNREGRWAEQLLNPCASAIIKGDATRVH 180

Query: 181 HLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSS-----SSTLAPVVNFASTDAR 240
           HL+CVLQELASPTGDANHRLADHGLRALAHHLSSNSSSS     SST+AP V FASTD R
Sbjct: 181 HLLCVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSFSSYSSTVAP-VTFASTDPR 240

Query: 241 FFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEA 300
           FFQRSLIKFHEVSPWFA PNNIANSSILH LSEEPN  RNLHILDIGVSHGVQWPTLLEA
Sbjct: 241 FFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRPRNLHILDIGVSHGVQWPTLLEA 300

Query: 301 LTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRL 360
           LTRRSGGPP LIRLTV+ PT+EHDQ  ETPFSIGPPGDNISSRLLSFAKSLNINLQINRL
Sbjct: 301 LTRRSGGPPSLIRLTVIGPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQINRL 360

Query: 361 DNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSEN 420
           +NHSLQ+LNSQVI KF DEILIVCA FRLHQLKH  PDER EFL+NLRKMEP AVILSEN
Sbjct: 361 NNHSLQSLNSQVINKFPDEILIVCAQFRLHQLKHCDPDERFEFLQNLRKMEPKAVILSEN 420

Query: 421 NIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMN 480
           N+ CSCSNCGNFD  FTRRVEYLWRFLDSTS+AFKGRES+ERRVMEGEAAKALTN GEMN
Sbjct: 421 NMGCSCSNCGNFDTRFTRRVEYLWRFLDSTSAAFKGRESEERRVMEGEAAKALTNDGEMN 480

Query: 481 EEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPIS 540
           EEK KW ERMRNAGF RK FAE TIDTARASMRRYDNNWEMR+EEKDGC+GLWWKGQP+S
Sbjct: 481 EEKGKWCERMRNAGFERKLFAEDTIDTARASMRRYDNNWEMRIEEKDGCLGLWWKGQPVS 540

Query: 541 FCSFWKLGAKPN 543
           FCSFWKLG K N
Sbjct: 541 FCSFWKLGIKSN 548

BLAST of Moc09g02010 vs. NCBI nr
Match: XP_004141813.1 (protein NODULATION SIGNALING PATHWAY 1 [Cucumis sativus] >KGN45491.1 hypothetical protein Csa_015542 [Cucumis sativus])

HSP 1 Score: 897.5 bits (2318), Expect = 5.7e-257
Identity = 460/556 (82.73%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDS PFFS FLDET NSSSINCYQWWDE+Q+ G+DLINGCL
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 60

Query: 61  SSSPAAATTDSTTPPNTT---SLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 120
           S+SP   T  ST  PNT    SLTPSDL+KKRKAPDD+ HK +Q HQNPRKNQNNQSKN 
Sbjct: 61  SNSP--TTVVSTRQPNTPTSHSLTPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNS 120

Query: 121 ADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG G      V+KKSVGNKK++SKSTGNNYN+GSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 121 ADKGSGAVEGVTVMKKSVGNKKNASKSTGNNYNSGSNKEGRWAEQLLNPCANAIVKGDAT 180

Query: 181 RVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSS-----SSTLAPVVNFAST 240
           RVHHL+CVLQELASPTGDANHRLADHGLRALA+HLSSNSSSS     SST+AP   FAST
Sbjct: 181 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVAP-FTFAST 240

Query: 241 DARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTL 300
           D RFFQRSLIKFHEVSPWFA PNNIANSSILH LSEE N  RNLHILDIGVSHGVQWPTL
Sbjct: 241 DPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHILDIGVSHGVQWPTL 300

Query: 301 LEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQI 360
           LEALTRRSGGPPPLIRLTV+APT+EHDQ  ETPFSIGPPGDNISSRLLSFAKSLNINLQI
Sbjct: 301 LEALTRRSGGPPPLIRLTVIAPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQI 360

Query: 361 NRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVIL 420
           NRLD HSLQ+LNSQ I K RDEILIVCA FRLHQLKH APDER EFL NLRKMEP AVIL
Sbjct: 361 NRLDIHSLQSLNSQAINKSRDEILIVCAQFRLHQLKHSAPDERQEFLENLRKMEPKAVIL 420

Query: 421 SENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTN-Q 480
           SENN+ CSCS CGNF++ F R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N  
Sbjct: 421 SENNMGCSCSKCGNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVD 480

Query: 481 GEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKG 540
           GEMNEEK KW ERMRN GF RK F E TIDTARASMRRYDNNWEMRME+KDGCVGLWWKG
Sbjct: 481 GEMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKG 540

Query: 541 QPISFCSFWKLGAKPN 543
           QP+SFCSFWKLG K N
Sbjct: 541 QPVSFCSFWKLGIKSN 553

BLAST of Moc09g02010 vs. NCBI nr
Match: XP_023514155.1 (nodulation-signaling pathway 1 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 897.1 bits (2317), Expect = 7.4e-257
Identity = 461/543 (84.90%), Postives = 490/543 (90.24%), Query Frame = 0

Query: 13  DHILDWLEDSAPFF-SPFLDETYNSSSINCYQWWDESQNIGQDLINGCLSSSPAAATTDS 72
           DHILDWL DS PFF SPF D++YNSSSINCYQWWDE+Q+IGQDLINGCLSSSP   TT S
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADKGGGG---- 132
           T PPN ++   LTPSDL+KKRKAPDDT HK +Q  QN RKNQNNQSKNGADK  G     
Sbjct: 104 TKPPNNSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKNGADKSSGAVAGV 163

Query: 133 -VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICVLQE 192
            V+KKSVGNK++SSK+TG+N NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHL+CVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGSNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPTGDANHRLADHGLRALAHHLSSNS--SSSSSTLAPVVNFASTDARFFQRSLIKFHE 252
           LASPTGDANHRLA +GLRALAH+LSSNS  SSSSSTLAP V FASTD RFFQRSLIKFHE
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSNSSCSSSSSTLAP-VTFASTDPRFFQRSLIKFHE 283

Query: 253 VSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 312
           VSPWFA PNNIANSSILH LSEEPN SRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL
Sbjct: 284 VSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPL 343

Query: 313 IRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNSQ 372
           IRLTV+APT+EHDQ AETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDN SLQ+LNSQ
Sbjct: 344 IRLTVIAPTLEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNRSLQSLNSQ 403

Query: 373 VIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCGN 432
           VIGKF DEILIVCA FRLHQLKH APDER EFL+NLRKMEP AVILSENN+ACSC+NCGN
Sbjct: 404 VIGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKMEPKAVILSENNMACSCNNCGN 463

Query: 433 FDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERMR 492
           FD  FTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAAKAL N+GEMNEE EKW ERMR
Sbjct: 464 FDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAAKALRNEGEMNEEMEKWCERMR 523

Query: 493 NAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAKP 545
           NAGFARK F E TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+SFCSFWKLG K 
Sbjct: 524 NAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGIKS 582

BLAST of Moc09g02010 vs. ExPASy Swiss-Prot
Match: Q4VYC8 (Protein NODULATION SIGNALING PATHWAY 1 OS=Medicago truncatula OX=3880 GN=NSP1 PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.8e-176
Identity = 338/560 (60.36%), Postives = 405/560 (72.32%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNI-GQDLINGC 60
           MT+E   PN  SDHILDWLE S  FF  FLD+ YN+  I+ Y+ W+++Q+I  Q  I+  
Sbjct: 1   MTME---PNPTSDHILDWLEGSVSFFPSFLDDPYNNGYIHEYEIWNQNQDISNQYQIDAN 60

Query: 61  LSSSPAAATTD----STTPPNTTSLTP--------SDLSKKRKAPDDTGHKTTQPHQNPR 120
            +SS A  +T     ++T  +TTSL P        SDL KKR A D+   K  QP     
Sbjct: 61  TNSSNATNSTTNIVAASTTTSTTSLEPNSFNNIPFSDLPKKRNAEDELSLK-KQPQNQKN 120

Query: 121 KNQNNQSKNGADKGG----GGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCA 180
           K   ++  N +D G     G VV+KS GNKK ++K+ G+N NNG+NK+GRWAEQLLNPCA
Sbjct: 121 KRLKSRPMNESDNGDAALEGTVVRKSGGNKKGAAKANGSNSNNGNNKDGRWAEQLLNPCA 180

Query: 181 NAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVN 240
            AI  G+  RV HL+ VL ELAS TGDANHRLA HGLRAL HHL   SSSSSST +  + 
Sbjct: 181 VAITGGNLNRVQHLLYVLHELASTTGDANHRLAAHGLRALTHHL---SSSSSSTPSGTIT 240

Query: 241 FASTDARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQ 300
           FAST+ RFFQ+SL+KF+E SPWF+ PNNIAN+SIL  L+EEPN  R LHILDIGVSHGVQ
Sbjct: 241 FASTEPRFFQKSLLKFYEFSPWFSFPNNIANASILQVLAEEPNNLRTLHILDIGVSHGVQ 300

Query: 301 WPTLLEALTRRSGGPPPLIRLTVV--APTVEHDQTAETPFSIGPPGDNISSRLLSFAKSL 360
           WPT LEAL+RR GGPPPL+RLTVV  + + E+DQ  ETPFSIGP GD  SS LL +A+SL
Sbjct: 301 WPTFLEALSRRPGGPPPLVRLTVVNASSSTENDQNMETPFSIGPCGDTFSSGLLGYAQSL 360

Query: 361 NINLQINRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKME 420
           N+NLQI +LDNH LQ LN++ +    DE LIVCA FRLH L H  PDER+EFL+ LR ME
Sbjct: 361 NVNLQIKKLDNHPLQTLNAKSVDTSSDETLIVCAQFRLHHLNHNNPDERSEFLKVLRGME 420

Query: 421 PNAVILSENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAK 480
           P  VILSENN+ C CS+CG+F   F+RRVEYLWRFLDSTSSAFK R+SDER++MEGEAAK
Sbjct: 421 PKGVILSENNMECCCSSCGDFATGFSRRVEYLWRFLDSTSSAFKNRDSDERKMMEGEAAK 480

Query: 481 ALTNQGEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVG 540
           ALTNQ EMNE +EKW ERM+ AGFA + F E  ID  RA +R+YDNNWEM++EE    V 
Sbjct: 481 ALTNQREMNERREKWCERMKEAGFAGEVFGEDAIDGGRALLRKYDNNWEMKVEENSTSVE 540

Query: 541 LWWKGQPISFCSFWKLGAKP 542
           LWWK QP+SFCS WKL  +P
Sbjct: 541 LWWKSQPVSFCSLWKLDKQP 553

BLAST of Moc09g02010 vs. ExPASy Swiss-Prot
Match: A1DQP9 (Protein NODULATION SIGNALING PATHWAY 1 OS=Lotus japonicus OX=34305 GN=NSP1 PE=2 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.3e-174
Identity = 346/561 (61.68%), Postives = 403/561 (71.84%), Query Frame = 0

Query: 1   MTIEEPGPNH-PSDHILDWLEDSAPFFSPFLDETYNSSS-INCYQWWDESQNIGQDLING 60
           MT+E   PN   SDHILDWLE S  FF  FLDE  N+S  I  Y  WD+ Q       + 
Sbjct: 1   MTME---PNSTTSDHILDWLEGSVSFFPSFLDEPCNNSGYIQEYHLWDQYQT------DA 60

Query: 61  CLSSSPAA-----ATTDSTTPPNTTSLTP---------SDLSKKRKAPDDTGHKTTQPHQ 120
              SSP A     AT  +T+  +TTSL P         SDL KKR A D++  K   P Q
Sbjct: 61  NTGSSPNATNSTTATIVATSATSTTSLEPCGSNNNQPLSDLPKKRNATDESSLK---PPQ 120

Query: 121 NPRKNQNNQSKNGADKGGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCAN 180
           N  KN+  +++   +   G  V+K   NKK  +K+ G+N N+G++KEGRWAEQLLNPCA 
Sbjct: 121 N--KNKRIKTRPMNEPENGDAVRK---NKKGGAKANGSNCNSGNSKEGRWAEQLLNPCAA 180

Query: 181 AIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNF 240
           AI  G+  RV HL+ VL ELASPTGD NHRLA HGLRAL HHLS  SSSSS T +  + F
Sbjct: 181 AIAGGNVNRVQHLLYVLHELASPTGDPNHRLAAHGLRALTHHLS--SSSSSPTSSGTITF 240

Query: 241 ASTDARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNL-SRNLHILDIGVSHGVQ 300
           AST+ RFFQ+SL+KF+EVSPWF+ PNNIAN+SIL  L+EE N+ SR LHILDIGVSHGVQ
Sbjct: 241 ASTEPRFFQKSLLKFYEVSPWFSFPNNIANASILQVLAEEANITSRTLHILDIGVSHGVQ 300

Query: 301 WPTLLEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNI 360
           WPTLL+AL+RRSGGPP ++RLTVV  T E+DQ  ETPFS  PPG N   RLL +A+S+NI
Sbjct: 301 WPTLLDALSRRSGGPPSVVRLTVV--TAENDQNMETPFSKAPPGYNYYPRLLGYAQSINI 360

Query: 361 NLQINRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPN 420
           NLQINR++NHSLQ LN+Q I    DEILIVCA FRLH L H +PDER+EFL+ LR MEP 
Sbjct: 361 NLQINRIENHSLQTLNAQSISASPDEILIVCAQFRLHHLNHNSPDERSEFLKVLRNMEPR 420

Query: 421 AVILSENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKAL 480
            VILSENN  C CS CGNF   FTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKAL
Sbjct: 421 GVILSENNTECCCSGCGNFAAGFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKAL 480

Query: 481 TNQGEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLW 540
           TNQ EMNEEKEKW  RM+ AGFA + F E  +D  RA +R+YD+NWEM++EEK+  VGLW
Sbjct: 481 TNQREMNEEKEKWCGRMKEAGFAGEVFGEDAVDGGRALLRKYDSNWEMKVEEKNTSVGLW 540

Query: 541 WKGQPISFCSFWKLGAKPNGG 545
           WKGQP+SFCS WKL     GG
Sbjct: 541 WKGQPVSFCSLWKLDGNDQGG 540

BLAST of Moc09g02010 vs. ExPASy Swiss-Prot
Match: Q9LRW3 (Scarecrow-like protein 29 OS=Arabidopsis thaliana OX=3702 GN=SCL29 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-125
Identity = 264/539 (48.98%), Postives = 341/539 (63.27%), Query Frame = 0

Query: 4   EEPGPNHPSDHILDWLEDSAPFFS-PFLDETYNSSSINCYQWWDESQNIGQDLINGCLSS 63
           E   PN   DH+L WLEDS      P  D++Y     +  Q W+  Q   QD  +G + S
Sbjct: 5   ETEPPNQTLDHVLSWLEDSVSLSPLPGFDDSYLLHEFDGSQTWEWDQT--QDPEHGFIQS 64

Query: 64  -----SPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 123
                S A    ++T     T     DL        D   +  QP+   RK  ++     
Sbjct: 65  YSQDLSAAYVGCEATNLEVVTEAPSIDL--------DLPPEIQQPNDQSRKRSHDGFLEA 124

Query: 124 ADKGGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHL 183
                   VKKS  +K+ + KS+  +  +G NKEGRWAE+LLNPCA AI   +++RV H 
Sbjct: 125 QQ------VKKSARSKRKAIKSSEKSSKDG-NKEGRWAEKLLNPCALAITASNSSRVQHY 184

Query: 184 ICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLI 243
           +CVL ELAS +GDAN RLA  GLRAL HHLS  SSS SS+  PV  FAS + + FQ++L+
Sbjct: 185 LCVLSELASSSGDANRRLAAFGLRALQHHLS--SSSVSSSFWPVFTFASAEVKMFQKTLL 244

Query: 244 KFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGG 303
           KF+EVSPWFALPNN+ANS+IL  L+++P   ++LHI+DIGVSHG+QWPTLLEAL+ R  G
Sbjct: 245 KFYEVSPWFALPNNMANSAILQILAQDPKDKKDLHIIDIGVSHGMQWPTLLEALSCRLEG 304

Query: 304 PPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQN 363
           PPP +R+TV++     D TA+ PFS+GPPG N  S+LL FA+SL INLQI+ LD      
Sbjct: 305 PPPRVRITVIS-----DLTADIPFSVGPPGYNYGSQLLGFARSLKINLQISVLD------ 364

Query: 364 LNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCS 423
              Q+I     E LIVCA FRLH LKH   DER E L+ +R + P  V+L ENN  CS S
Sbjct: 365 -KLQLIDTSPHENLIVCAQFRLHHLKHSINDERGETLKAVRSLRPKGVVLCENNGECSSS 424

Query: 424 NCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWY 483
              +F   F++++EY+W+FLDSTSS FK   S+ER++MEGEA K L N G+MNE KEKWY
Sbjct: 425 --ADFAAGFSKKLEYVWKFLDSTSSGFKEENSEERKLMEGEATKVLMNAGDMNEGKEKWY 484

Query: 484 ERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWK 537
           ERMR AGF  + F E  +D A++ +R+YDNNWE+RME+ D   GL WKG+ +SFCS WK
Sbjct: 485 ERMREAGFFVEAFEEDAVDGAKSLLRKYDNNWEIRMEDGDTFAGLMWKGEAVSFCSLWK 510

BLAST of Moc09g02010 vs. ExPASy Swiss-Prot
Match: Q84MQ9 (Protein NODULATION SIGNALING PATHWAY 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP1 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.1e-85
Identity = 216/521 (41.46%), Postives = 285/521 (54.70%), Query Frame = 0

Query: 44  WWDESQNIGQDLINGCL--------SSSPAAATTDSTTP-PNTTSLTPSDLSKKRKAPDD 103
           WW  S    QD I   +        +++PAAA+    +P  ++ S  PS  SKKRK+P  
Sbjct: 46  WWAASPAAQQDDIGSVVAQTLSPPSTAAPAAASPSIASPAASSPSDVPSSSSKKRKSP-- 105

Query: 104 TGHKTTQPHQNPRKNQNNQSKNGADKGGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRW 163
                   H+ P        K G  KGGGG                      GS+++ RW
Sbjct: 106 -------AHRAP--GHTGGKKGGGGKGGGG----------------------GSDRDMRW 165

Query: 164 AEQLLNPCANAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSS 223
           AEQLLNPCA A+  G+ +RV HL  VL EL S +GDANHRLA HGLRALA  L +    +
Sbjct: 166 AEQLLNPCAVAVEAGNLSRVQHLFYVLGELESFSGDANHRLAAHGLRALARWLPAAVGPA 225

Query: 224 SSTLA--------PVVNFASTDARFFQRSLIKFHEVSPWFALPNNIANSSILH--TLSEE 283
           ++           P   FA+ + R F+ SLI+FHEVSPWFALPN +AN++I    T    
Sbjct: 226 AAAAVRVPPCSERPTTAFAAAEPRLFRASLIRFHEVSPWFALPNALANAAIAQASTCGAA 285

Query: 284 PNLSRNLHILDIGVSHGVQWPTLLEALTRRSGG-PPPLIRLTVVAPTVEHDQTAETPFSI 343
               R LH++D+GVSHGVQWPTLLE+LTR+ GG  PP +RLTVV P          PFS 
Sbjct: 286 GATPRPLHVVDLGVSHGVQWPTLLESLTRQPGGRAPPSVRLTVVGPGATATSPV-APFSA 345

Query: 344 GPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLK 403
            PPG + S  LL +AKS+N++L+I+R        L+  V G    E L+VC  FR   L 
Sbjct: 346 SPPGYDFSPHLLRYAKSINLDLRISR-----AATLDDAVPGD-DGEALVVCLQFR---LG 405

Query: 404 HRAPDERTEFLRNLRKMEPNAVILSE--NNIACSCSNCGNFDITFTRRVEYLWRFLDSTS 463
           H A +ER E LR  R + P  V+LSE  + +     + G+    F  R+E LWRFL+STS
Sbjct: 406 HAAAEERREVLRKARGLNPELVVLSELDSGVGVVGGDGGSAAGEFAARLELLWRFLESTS 465

Query: 464 SAFKGRESDERRVMEGEAAK--ALTNQGEMNEEKEKWYERMRNAGFARKFFAEGTIDTAR 523
           +AFKG++ +ERR++E EA    A  +     E +E W ERM  AGF    F    +++AR
Sbjct: 466 AAFKGKDVEERRLLEAEAGAILAAADVAAAGEGREGWRERMAAAGFEEAPFGAEAVESAR 523

Query: 524 ASMRRYDNNWEMRM-EEKDGCVGLWWKGQPISFCSFWKLGA 540
           + +R+YD+ WEM         V L WKGQP+SFCS W+  A
Sbjct: 526 SLLRKYDSGWEMSAPSPAAAAVALRWKGQPVSFCSLWRPAA 523

BLAST of Moc09g02010 vs. ExPASy Swiss-Prot
Match: Q9SN22 (Scarecrow-like protein 32 OS=Arabidopsis thaliana OX=3702 GN=SCL32 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 3.3e-34
Identity = 116/403 (28.78%), Postives = 182/403 (45.16%), Query Frame = 0

Query: 151 EGRWAEQLLNPCANAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSN 210
           +  + EQLL  CA AI   DA   H ++ VL  +A P GD+  RL    LRAL     S 
Sbjct: 23  DANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRALLSRAVSK 82

Query: 211 SSSSSSTLAPVVNFASTDARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRN 270
           + + SST++  +  A    RF    L  F +++PW       AN++IL  +         
Sbjct: 83  TPTLSSTIS-FLPQADELHRFSVVELAAFVDLTPWHRFGFIAANAAILTAVEG----YST 142

Query: 271 LHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNI 330
           +HI+D+ ++H +Q PTL++A+  R   PPPL++LTVV+       +   P  I    + +
Sbjct: 143 VHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVS------SSDHFPPFINISYEEL 202

Query: 331 SSRLLSFAKSLNINLQINRLDNHSLQNLNS-----QVIGKFRDEILIVCAHFRL-----H 390
            S+L++FA + NI ++   + +      +S     ++     +E L+V  H  L      
Sbjct: 203 GSKLVNFATTRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVVNCHMMLRYIPEE 262

Query: 391 QLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCGNFDITFTRRVEYLWRFLDST 450
            L   +   RT FL+ LR + P  V L E ++  +  N  N          Y W   D+T
Sbjct: 263 PLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVN---RLKSAFNYFWIPFDTT 322

Query: 451 SSAFKGRESDERRVMEGE--------AAKALTNQGEMNEEKEKWYERMRNAGFARKFFAE 510
            +      S++RR  E E         AK    + E  E K +W ERMR A F      E
Sbjct: 323 DTFM----SEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFGGVRVKE 382

Query: 511 GTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFW 536
             +   +A +  +   W M+ E+ D  + L WKG  + F + W
Sbjct: 383 DAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHSVVFATVW 407

BLAST of Moc09g02010 vs. ExPASy TrEMBL
Match: A0A6J1D4T6 (nodulation-signaling pathway 1 protein OS=Momordica charantia OX=3673 GN=LOC111017018 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 543/544 (99.82%), Postives = 543/544 (99.82%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60
           MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL
Sbjct: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60

Query: 61  SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK 120
           SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK
Sbjct: 61  SSSPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADK 120

Query: 121 GGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICV 180
           GGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICV
Sbjct: 121 GGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICV 180

Query: 181 LQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFH 240
           LQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFH
Sbjct: 181 LQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFH 240

Query: 241 EVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPP 300
           EVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPP
Sbjct: 241 EVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPP 300

Query: 301 LIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNS 360
           LIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNS
Sbjct: 301 LIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNS 360

Query: 361 QVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCG 420
           QVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCG
Sbjct: 361 QVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCG 420

Query: 421 NFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERM 480
           NFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERM
Sbjct: 421 NFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERM 480

Query: 481 RNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAK 540
           RNAGFARKFFAE TIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAK
Sbjct: 481 RNAGFARKFFAEXTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAK 540

Query: 541 PNGG 545
           PNGG
Sbjct: 541 PNGG 544

BLAST of Moc09g02010 vs. ExPASy TrEMBL
Match: A0A6J1KER4 (nodulation-signaling pathway 1 protein OS=Cucurbita maxima OX=3661 GN=LOC111494340 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.0e-264
Identity = 472/555 (85.05%), Postives = 501/555 (90.27%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFF-SPFLDETYNSSSINCYQWWDESQNIGQDLINGC 60
           MTIEEPG NHPSDHILDWL DS PFF SPF D++YNSSSINCYQWWDE+Q+IGQDLINGC
Sbjct: 40  MTIEEPGANHPSDHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGC 99

Query: 61  LSSSPAAATTDSTTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKN 120
           LSSSP   TT ST PPNT++   LTPSDL+KKRKAPDDT HK +Q  QN RKNQNNQSKN
Sbjct: 100 LSSSP---TTVSTKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSKN 159

Query: 121 GADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDA 180
           GADKG G      V+KKSVGNK++SSK+TGNN +NGSNKEGRWAEQLLNPCANAIIKGDA
Sbjct: 160 GADKGSGAVEGVTVIKKSVGNKRNSSKATGNNNHNGSNKEGRWAEQLLNPCANAIIKGDA 219

Query: 181 TRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSS--SSSSTLAPVVNFASTDA 240
           TRVHHL+CVLQELASPTGDANHRLA +GLRALAH+LSSNSS  SSSST+AP V FASTD 
Sbjct: 220 TRVHHLLCVLQELASPTGDANHRLAAYGLRALAHYLSSNSSCYSSSSTVAP-VTFASTDL 279

Query: 241 RFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLE 300
           RFFQRSLIKFHEVSPWFA PNNIANSSILH LSEEPN SRNLHILDIGVSHGVQWPTLLE
Sbjct: 280 RFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLE 339

Query: 301 ALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 360
           ALTRRSGGPPPLIRLTV+APTVEHDQ AETPFSIGPPGDNISSRLLSFAKSLNINLQINR
Sbjct: 340 ALTRRSGGPPPLIRLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINR 399

Query: 361 LDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSE 420
           LDN SLQ+ NSQVIGK  DEILIVCA FRLHQLKH APDER EFL+NLRK+EP AVILSE
Sbjct: 400 LDNRSLQSFNSQVIGKLPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSE 459

Query: 421 NNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEM 480
           NN+ACSC+NCGNFD  FTR+VEYLWRFLDSTSSAFKGRES+ER+VMEGEAA+ LTNQGEM
Sbjct: 460 NNMACSCNNCGNFDTGFTRKVEYLWRFLDSTSSAFKGRESEERKVMEGEAARGLTNQGEM 519

Query: 481 NEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPI 540
           NEE EKW ERMRNAGFARK F E TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+
Sbjct: 520 NEEMEKWCERMRNAGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPV 579

Query: 541 SFCSFWKLGAKPNGG 545
           SFCSFWKLG K NGG
Sbjct: 580 SFCSFWKLGMKSNGG 590

BLAST of Moc09g02010 vs. ExPASy TrEMBL
Match: A0A0A0KCK6 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450500 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 460/556 (82.73%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60
           MTIEE GPNHPSDHILDWLEDS PFFS FLDET NSSSINCYQWWDE+Q+ G+DLINGCL
Sbjct: 1   MTIEETGPNHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 60

Query: 61  SSSPAAATTDSTTPPNTT---SLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 120
           S+SP   T  ST  PNT    SLTPSDL+KKRKAPDD+ HK +Q HQNPRKNQNNQSKN 
Sbjct: 61  SNSP--TTVVSTRQPNTPTSHSLTPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNS 120

Query: 121 ADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG G      V+KKSVGNKK++SKSTGNNYN+GSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 121 ADKGSGAVEGVTVMKKSVGNKKNASKSTGNNYNSGSNKEGRWAEQLLNPCANAIVKGDAT 180

Query: 181 RVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSS-----SSTLAPVVNFAST 240
           RVHHL+CVLQELASPTGDANHRLADHGLRALA+HLSSNSSSS     SST+AP   FAST
Sbjct: 181 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVAP-FTFAST 240

Query: 241 DARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTL 300
           D RFFQRSLIKFHEVSPWFA PNNIANSSILH LSEE N  RNLHILDIGVSHGVQWPTL
Sbjct: 241 DPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHILDIGVSHGVQWPTL 300

Query: 301 LEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQI 360
           LEALTRRSGGPPPLIRLTV+APT+EHDQ  ETPFSIGPPGDNISSRLLSFAKSLNINLQI
Sbjct: 301 LEALTRRSGGPPPLIRLTVIAPTIEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQI 360

Query: 361 NRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVIL 420
           NRLD HSLQ+LNSQ I K RDEILIVCA FRLHQLKH APDER EFL NLRKMEP AVIL
Sbjct: 361 NRLDIHSLQSLNSQAINKSRDEILIVCAQFRLHQLKHSAPDERQEFLENLRKMEPKAVIL 420

Query: 421 SENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTN-Q 480
           SENN+ CSCS CGNF++ F R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N  
Sbjct: 421 SENNMGCSCSKCGNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVD 480

Query: 481 GEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKG 540
           GEMNEEK KW ERMRN GF RK F E TIDTARASMRRYDNNWEMRME+KDGCVGLWWKG
Sbjct: 481 GEMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKG 540

Query: 541 QPISFCSFWKLGAKPN 543
           QP+SFCSFWKLG K N
Sbjct: 541 QPVSFCSFWKLGIKSN 553

BLAST of Moc09g02010 vs. ExPASy TrEMBL
Match: A0A6J1HKE2 (nodulation-signaling pathway 1 protein OS=Cucurbita moschata OX=3662 GN=LOC111464355 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.5e-255
Identity = 458/542 (84.50%), Postives = 490/542 (90.41%), Query Frame = 0

Query: 13  DHILDWLEDSAPFF-SPFLDETYNSSSINCYQWWDESQNIGQDLINGCLSSSPAAATTDS 72
           DHILDWL DS PFF SPF D++YNSSSINCYQWWDE+Q+IGQDLINGCLSSSP   TT S
Sbjct: 44  DHILDWLADSVPFFSSPFPDDSYNSSSINCYQWWDENQDIGQDLINGCLSSSP---TTVS 103

Query: 73  TTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNGADKGGGG---- 132
           T PPNT++   LTPSDL+KKRKAPDDT HK +Q  QN RKNQNNQS+NGADK  G     
Sbjct: 104 TKPPNTSTSHHLTPSDLTKKRKAPDDTVHKPSQTQQNQRKNQNNQSRNGADKSSGAVAGV 163

Query: 133 -VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICVLQE 192
            V+KKSVGNK++SSK+TGNN NNG+NKEGRWAEQLLNPCANAIIKGDATRVHHL+CVLQE
Sbjct: 164 TVMKKSVGNKRNSSKATGNNNNNGNNKEGRWAEQLLNPCANAIIKGDATRVHHLLCVLQE 223

Query: 193 LASPTGDANHRLADHGLRALAHHLSSNSS-SSSSTLAPVVNFASTDARFFQRSLIKFHEV 252
           LASPTGDANHRLA +GLRALAH+LSSNSS SSSSTLAP V FASTD RFFQRSLIKFHEV
Sbjct: 224 LASPTGDANHRLAAYGLRALAHYLSSNSSCSSSSTLAP-VTFASTDPRFFQRSLIKFHEV 283

Query: 253 SPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLI 312
           SPWFA PNNIANSSILH LSEEPN SRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLI
Sbjct: 284 SPWFAFPNNIANSSILHILSEEPNRSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLI 343

Query: 313 RLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNSQV 372
           RLTV+APTVEHDQ AETPFSIGPPGDNISSRLLSFAKSLNINLQINRLD+ SL +LN+QV
Sbjct: 344 RLTVIAPTVEHDQNAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDSLSLLSLNAQV 403

Query: 373 IGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCGNF 432
           IGKF DEILIVCA FRLHQLKH APDER EFL+NLRK+EP AVILSENN+ACSC+NCGNF
Sbjct: 404 IGKFPDEILIVCAQFRLHQLKHYAPDERFEFLQNLRKIEPKAVILSENNMACSCTNCGNF 463

Query: 433 DITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWYERMRN 492
           D  FTR+VEYLWRFLDSTSSAFKGRES+ER++MEGEAAKAL N+GEMNEE EKW ERMRN
Sbjct: 464 DTGFTRKVEYLWRFLDSTSSAFKGRESEERKLMEGEAAKALRNEGEMNEEMEKWCERMRN 523

Query: 493 AGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKLGAKPN 545
           AGFARK F E TIDTARASMRRYDNNWEMR+EEKDGCVGLWWKGQP+SFCSFWKLG K N
Sbjct: 524 AGFARKLFGEDTIDTARASMRRYDNNWEMRVEEKDGCVGLWWKGQPVSFCSFWKLGMKSN 581

BLAST of Moc09g02010 vs. ExPASy TrEMBL
Match: A0A1S3CGQ3 (nodulation-signaling pathway 1 protein OS=Cucumis melo OX=3656 GN=LOC103500695 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.2e-254
Identity = 454/556 (81.65%), Postives = 487/556 (87.59%), Query Frame = 0

Query: 1   MTIEEPGPNHPSDHILDWLEDSAPFFSPFLDETYNSSSINCYQWWDESQNIGQDLINGCL 60
           MTIEE GP+HPSDHILDWLEDS PFFS FLDET NSSSINCYQWWDE+Q+ G+DLINGCL
Sbjct: 44  MTIEETGPDHPSDHILDWLEDSVPFFSSFLDETSNSSSINCYQWWDENQDTGEDLINGCL 103

Query: 61  SSSPAAATTDSTTPPNTTS---LTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 120
           S+SP   T  ST  PNT +   L PSDL+KKRKAPDD+ HK +Q HQNPRKNQNNQSKN 
Sbjct: 104 SNSP--TTVVSTRQPNTPTSHHLPPSDLTKKRKAPDDSVHKKSQTHQNPRKNQNNQSKNI 163

Query: 121 ADKGGGG-----VVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDAT 180
           ADKG G      V+KKSVGNKK++SKSTGNNYNNGSNKEGRWAEQLLNPCANAI+KGDAT
Sbjct: 164 ADKGSGAVEGVTVIKKSVGNKKNASKSTGNNYNNGSNKEGRWAEQLLNPCANAIVKGDAT 223

Query: 181 RVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSS-----SSTLAPVVNFAST 240
           RVHHL+CVLQELASPTGDANHRLADHGLRALA+HLSSNSSSS     SST++P + FAST
Sbjct: 224 RVHHLLCVLQELASPTGDANHRLADHGLRALAYHLSSNSSSSSFSSYSSTVSP-ITFAST 283

Query: 241 DARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTL 300
           D RFFQRSLIKFHEVSPWFA PNNIANSSILH LSEE N  RNLH+LDIGVSHGVQWPTL
Sbjct: 284 DPRFFQRSLIKFHEVSPWFAFPNNIANSSILHILSEESNRPRNLHVLDIGVSHGVQWPTL 343

Query: 301 LEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQI 360
           LEALTRRSGGPPPLIRLTV+APTVEHDQ  ETPFSIGPPGDNISSRLLSFAKSLNINLQI
Sbjct: 344 LEALTRRSGGPPPLIRLTVIAPTVEHDQNTETPFSIGPPGDNISSRLLSFAKSLNINLQI 403

Query: 361 NRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVIL 420
           NRLD HSLQ+LNSQ I K RDEILIVC+ FRLHQLKH APDER EFL+NLRKMEP AVIL
Sbjct: 404 NRLDIHSLQSLNSQAINKSRDEILIVCSQFRLHQLKHSAPDERQEFLQNLRKMEPKAVIL 463

Query: 421 SENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTN-Q 480
           SENN+ CSCS C NF++ F R VEY+W+FLDSTS+AFKGRES+ERRVMEGEAAKAL N +
Sbjct: 464 SENNMGCSCSKCVNFEMGFRRGVEYVWKFLDSTSAAFKGRESEERRVMEGEAAKALRNVE 523

Query: 481 GEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKG 540
           GEMNEEK KW ERMRN GF RK F E TIDTARASMRRYDNNWEMRME+KDGCVGLWWKG
Sbjct: 524 GEMNEEKGKWCERMRNVGFERKRFGEDTIDTARASMRRYDNNWEMRMEDKDGCVGLWWKG 583

Query: 541 QPISFCSFWKLGAKPN 543
           QP+SFCS WKLG K N
Sbjct: 584 QPVSFCSLWKLGIKSN 596

BLAST of Moc09g02010 vs. TAIR 10
Match: AT3G13840.1 (GRAS family transcription factor )

HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-126
Identity = 264/539 (48.98%), Postives = 341/539 (63.27%), Query Frame = 0

Query: 4   EEPGPNHPSDHILDWLEDSAPFFS-PFLDETYNSSSINCYQWWDESQNIGQDLINGCLSS 63
           E   PN   DH+L WLEDS      P  D++Y     +  Q W+  Q   QD  +G + S
Sbjct: 5   ETEPPNQTLDHVLSWLEDSVSLSPLPGFDDSYLLHEFDGSQTWEWDQT--QDPEHGFIQS 64

Query: 64  -----SPAAATTDSTTPPNTTSLTPSDLSKKRKAPDDTGHKTTQPHQNPRKNQNNQSKNG 123
                S A    ++T     T     DL        D   +  QP+   RK  ++     
Sbjct: 65  YSQDLSAAYVGCEATNLEVVTEAPSIDL--------DLPPEIQQPNDQSRKRSHDGFLEA 124

Query: 124 ADKGGGGVVKKSVGNKKSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHL 183
                   VKKS  +K+ + KS+  +  +G NKEGRWAE+LLNPCA AI   +++RV H 
Sbjct: 125 QQ------VKKSARSKRKAIKSSEKSSKDG-NKEGRWAEKLLNPCALAITASNSSRVQHY 184

Query: 184 ICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLI 243
           +CVL ELAS +GDAN RLA  GLRAL HHLS  SSS SS+  PV  FAS + + FQ++L+
Sbjct: 185 LCVLSELASSSGDANRRLAAFGLRALQHHLS--SSSVSSSFWPVFTFASAEVKMFQKTLL 244

Query: 244 KFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGG 303
           KF+EVSPWFALPNN+ANS+IL  L+++P   ++LHI+DIGVSHG+QWPTLLEAL+ R  G
Sbjct: 245 KFYEVSPWFALPNNMANSAILQILAQDPKDKKDLHIIDIGVSHGMQWPTLLEALSCRLEG 304

Query: 304 PPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQN 363
           PPP +R+TV++     D TA+ PFS+GPPG N  S+LL FA+SL INLQI+ LD      
Sbjct: 305 PPPRVRITVIS-----DLTADIPFSVGPPGYNYGSQLLGFARSLKINLQISVLD------ 364

Query: 364 LNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCS 423
              Q+I     E LIVCA FRLH LKH   DER E L+ +R + P  V+L ENN  CS S
Sbjct: 365 -KLQLIDTSPHENLIVCAQFRLHHLKHSINDERGETLKAVRSLRPKGVVLCENNGECSSS 424

Query: 424 NCGNFDITFTRRVEYLWRFLDSTSSAFKGRESDERRVMEGEAAKALTNQGEMNEEKEKWY 483
              +F   F++++EY+W+FLDSTSS FK   S+ER++MEGEA K L N G+MNE KEKWY
Sbjct: 425 --ADFAAGFSKKLEYVWKFLDSTSSGFKEENSEERKLMEGEATKVLMNAGDMNEGKEKWY 484

Query: 484 ERMRNAGFARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWK 537
           ERMR AGF  + F E  +D A++ +R+YDNNWE+RME+ D   GL WKG+ +SFCS WK
Sbjct: 485 ERMREAGFFVEAFEEDAVDGAKSLLRKYDNNWEIRMEDGDTFAGLMWKGEAVSFCSLWK 510

BLAST of Moc09g02010 vs. TAIR 10
Match: AT3G49950.1 (GRAS family transcription factor )

HSP 1 Score: 147.9 bits (372), Expect = 2.4e-35
Identity = 116/403 (28.78%), Postives = 182/403 (45.16%), Query Frame = 0

Query: 151 EGRWAEQLLNPCANAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSN 210
           +  + EQLL  CA AI   DA   H ++ VL  +A P GD+  RL    LRAL     S 
Sbjct: 23  DANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRALLSRAVSK 82

Query: 211 SSSSSSTLAPVVNFASTDARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRN 270
           + + SST++  +  A    RF    L  F +++PW       AN++IL  +         
Sbjct: 83  TPTLSSTIS-FLPQADELHRFSVVELAAFVDLTPWHRFGFIAANAAILTAVEG----YST 142

Query: 271 LHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVVAPTVEHDQTAETPFSIGPPGDNI 330
           +HI+D+ ++H +Q PTL++A+  R   PPPL++LTVV+       +   P  I    + +
Sbjct: 143 VHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVS------SSDHFPPFINISYEEL 202

Query: 331 SSRLLSFAKSLNINLQINRLDNHSLQNLNS-----QVIGKFRDEILIVCAHFRL-----H 390
            S+L++FA + NI ++   + +      +S     ++     +E L+V  H  L      
Sbjct: 203 GSKLVNFATTRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVVNCHMMLRYIPEE 262

Query: 391 QLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCGNFDITFTRRVEYLWRFLDST 450
            L   +   RT FL+ LR + P  V L E ++  +  N  N          Y W   D+T
Sbjct: 263 PLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVN---RLKSAFNYFWIPFDTT 322

Query: 451 SSAFKGRESDERRVMEGE--------AAKALTNQGEMNEEKEKWYERMRNAGFARKFFAE 510
            +      S++RR  E E         AK    + E  E K +W ERMR A F      E
Sbjct: 323 DTFM----SEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFGGVRVKE 382

Query: 511 GTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFW 536
             +   +A +  +   W M+ E+ D  + L WKG  + F + W
Sbjct: 383 DAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHSVVFATVW 407

BLAST of Moc09g02010 vs. TAIR 10
Match: AT4G37650.1 (GRAS family transcription factor )

HSP 1 Score: 141.7 bits (356), Expect = 1.7e-33
Identity = 115/401 (28.68%), Postives = 181/401 (45.14%), Query Frame = 0

Query: 153 RWAEQLLNPCANAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSS 212
           +WA+ +L   A A    D  R   ++  L EL+SP GD   +LA + L+AL + ++ +  
Sbjct: 141 KWADSVLLEAARAFSDKDTARAQQILWTLNELSSPYGDTEQKLASYFLQALFNRMTGSGE 200

Query: 213 SSSSTLAPVVNFAST-DARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNL 272
               T+        T      +++++KF EVSPW    +  AN +IL  +  E      +
Sbjct: 201 RCYRTMVTAAATEKTCSFESTRKTVLKFQEVSPWATFGHVAANGAILEAVDGE----AKI 260

Query: 273 HILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLT--VVAPTVEHDQTAETPFSIGPPGDN 332
           HI+DI  +   QWPTLLEAL  RS   P L RLT  VVA    +DQTA            
Sbjct: 261 HIVDISSTFCTQWPTLLEALATRSDDTPHL-RLTTVVVANKFVNDQTASHRMM-----KE 320

Query: 333 ISSRLLSFAKSLNINLQINRLDN-HSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPD 392
           I +R+  FA+ + +  + N + +   L   +   +    DE+L +     +H +  R   
Sbjct: 321 IGNRMEKFARLMGVPFKFNIIHHVGDLSEFDLNELDVKPDEVLAINCVGAMHGIASRG-S 380

Query: 393 ERTEFLRNLRKMEPNAVILSENNIACSCSNCGNFDITFTRRVEYLWRF----LDSTSSAF 452
            R   + + R++ P  V + E          G FD  F R      R+     +S   +F
Sbjct: 381 PRDAVISSFRRLRPRIVTVVEEEADLVGEEEGGFDDEFLRGFGECLRWFRVCFESWEESF 440

Query: 453 KGRESDERRVMEGEAAKAL--------TNQGEMNEEKEKWYERMRNAGFARKFFAEGTID 512
             R S+ER ++E  A +A+        ++  E  E   KW  RMRN+GF    +++   D
Sbjct: 441 P-RTSNERLMLERAAGRAIVDLVACEPSDSTERRETARKWSRRMRNSGFGAVGYSDEVAD 500

Query: 513 TARASMRRY-DNNWEMRMEEKDGCVGLWWKGQPISFCSFWK 537
             RA +RRY +  W M        + L W+ QP+ + S W+
Sbjct: 501 DVRALLRRYKEGVWSMVQCPDAAGIFLCWRDQPVVWASAWR 529

BLAST of Moc09g02010 vs. TAIR 10
Match: AT5G66770.1 (GRAS family transcription factor )

HSP 1 Score: 104.8 bits (260), Expect = 2.3e-22
Identity = 105/390 (26.92%), Postives = 167/390 (42.82%), Query Frame = 0

Query: 162 CANAIIKGDATRVHHLICVLQELASPTGDANHRLADHGLRALAHHLSSNSSSSSSTLAPV 221
           CA  I   D       +  ++E  S  GD   R+A +   AL++ LS NS ++SS     
Sbjct: 224 CAR-ISDSDPNEASKTLLQIRESVSELGDPTERVAFYFTEALSNRLSPNSPATSS----- 283

Query: 222 VNFASTDARFFQRSLIKFHEVSPWFALPNNIANSSILHTLSEEPNLSRNLHILDIGVSHG 281
              +S+       S    ++  P+    +  AN +IL    E    S  +HI+D G+  G
Sbjct: 284 ---SSSSTEDLILSYKTLNDACPYSKFAHLTANQAIL----EATEKSNKIHIVDFGIVQG 343

Query: 282 VQWPTLLEALTRRSGGPPPLIRLT-VVAPTVEHDQTAETPFSIGPPGDNISSRLLSFAKS 341
           +QWP LL+AL  R+ G P  IR++ + AP++      E+P    P      +RL  FAK 
Sbjct: 344 IQWPALLQALATRTSGKPTQIRVSGIPAPSL-----GESP---EPSLIATGNRLRDFAKV 403

Query: 342 LNINLQINRLDNHSLQNLNSQVIGKFRDEILIVCAHFRLHQLKHRAPDERTEFLRNLRKM 401
           L++N     +    +  LN        DE+L V    +L++L    P      LR  + +
Sbjct: 404 LDLNFDFIPILT-PIHLLNGSSFRVDPDEVLAVNFMLQLYKLLDETPTIVDTALRLAKSL 463

Query: 402 EPNAVILSENNIACSCSNCGNFDITFTRRVEYLWRFLDSTSSAFK---GRESDER----R 461
            P  V L E  ++ +        + F  RV+   +F  +   + +   GR+S+ER    R
Sbjct: 464 NPRVVTLGEYEVSLN-------RVGFANRVKNALQFYSAVFESLEPNLGRDSEERVRVER 523

Query: 462 VMEGEAAKALTN------QGEMNEEKEKWYERMRNAGFARKFFAEGTIDTARASMRRYD- 521
            + G     L          E  EEKE+W   M NAGF     +   +  A+  +  Y+ 
Sbjct: 524 ELFGRRISGLIGPEKTGIHRERMEEKEQWRVLMENAGFESVKLSNYAVSQAKILLWNYNY 583

Query: 522 NNWEMRMEEKDGCVGLWWKGQPISFCSFWK 537
           +N    +E K G + L W   P+   S W+
Sbjct: 584 SNLYSIVESKPGFISLAWNDLPLLTLSSWR 584

BLAST of Moc09g02010 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 103.6 bits (257), Expect = 5.1e-22
Identity = 99/413 (23.97%), Postives = 181/413 (43.83%), Query Frame = 0

Query: 134 KSSSKSTGNNYNNGSNKEGRWAEQLLNPCANAIIKGDATRVHHLICVLQELASPTGDANH 193
           +SS +ST +     S + G      L  CA AI + +      L+  +  LA     A  
Sbjct: 159 ESSDESTRSVVLVDSQETGVRLVHALVACAEAIHQENLNLADALVKRVGTLAGSQAGAMG 218

Query: 194 RLADHGLRALAHHLSSNSSSSSSTLAPVVNFASTDARFFQRSLIKFHEVSPWFALPNNIA 253
           ++A +  +ALA  +  + ++ +   A V      +  F +   + F+E  P+    +  A
Sbjct: 219 KVATYFAQALARRIYRDYTAETDVCAAV------NPSFEEVLEMHFYESCPYLKFAHFTA 278

Query: 254 NSSILHTLSEEPNLSRNLHILDIGVSHGVQWPTLLEALTRRSGGPPPLIRLTVVAPTVEH 313
           N +IL    E    +R +H++D+G++ G+QWP L++AL  R GGPP      +  P  E+
Sbjct: 279 NQAIL----EAVTTARRVHVIDLGLNQGMQWPALMQALALRPGGPPSFRLTGIGPPQTEN 338

Query: 314 DQTAETPFSIGPPGDNISSRLLSFAKSLNINLQINRLDNHSLQNLNSQVI-GKFRDEILI 373
             + +           +  +L  FA+++ +  +   L   SL +L  ++   +   E L+
Sbjct: 339 SDSLQ----------QLGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESETLV 398

Query: 374 VCAHFRLHQLKHRAPDERTEFLRNLRKMEPNAVILSENNIACSCSNCGNFDITFTRRVEY 433
           V + F LH+L  R+     + L  ++ ++P+ V + E     +  N   F   F   + Y
Sbjct: 399 VNSVFELHRLLARS-GSIEKLLNTVKAIKPSIVTVVEQE---ANHNGIVFLDRFNEALHY 458

Query: 434 LWRFLDSTSSAFKGRESDE--------RRVMEGEAAKALTNQGEMNEEKEKWYERMRNAG 493
                DS   ++     D         R+++   AA+  +++ E +E   +W  RM++AG
Sbjct: 459 YSSLFDSLEDSYSLPSQDRVMSEVYLGRQILNVVAAEG-SDRVERHETAAQWRIRMKSAG 518

Query: 494 FARKFFAEGTIDTARASMRRYDNNWEMRMEEKDGCVGLWWKGQPISFCSFWKL 538
           F            A   +  Y      R+EE DGC+ + W+ +P+   S WKL
Sbjct: 519 FDPIHLGSSAFKQASMLLSLYATGDGYRVEENDGCLMIGWQTRPLITTSAWKL 546

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148342.10.0e+0099.82nodulation-signaling pathway 1 protein [Momordica charantia][more]
XP_023000031.12.1e-26485.05nodulation-signaling pathway 1 protein [Cucurbita maxima][more]
XP_038897214.11.3e-25883.33protein NODULATION SIGNALING PATHWAY 1 [Benincasa hispida][more]
XP_004141813.15.7e-25782.73protein NODULATION SIGNALING PATHWAY 1 [Cucumis sativus] >KGN45491.1 hypothetica... [more]
XP_023514155.17.4e-25784.90nodulation-signaling pathway 1 protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q4VYC81.8e-17660.36Protein NODULATION SIGNALING PATHWAY 1 OS=Medicago truncatula OX=3880 GN=NSP1 PE... [more]
A1DQP91.3e-17461.68Protein NODULATION SIGNALING PATHWAY 1 OS=Lotus japonicus OX=34305 GN=NSP1 PE=2 ... [more]
Q9LRW31.8e-12548.98Scarecrow-like protein 29 OS=Arabidopsis thaliana OX=3702 GN=SCL29 PE=2 SV=1[more]
Q84MQ91.1e-8541.46Protein NODULATION SIGNALING PATHWAY 1 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SN223.3e-3428.78Scarecrow-like protein 32 OS=Arabidopsis thaliana OX=3702 GN=SCL32 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D4T60.0e+0099.82nodulation-signaling pathway 1 protein OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1KER41.0e-26485.05nodulation-signaling pathway 1 protein OS=Cucurbita maxima OX=3661 GN=LOC1114943... [more]
A0A0A0KCK62.7e-25782.73GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450500 PE=3 S... [more]
A0A6J1HKE21.5e-25584.50nodulation-signaling pathway 1 protein OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A1S3CGQ32.2e-25481.65nodulation-signaling pathway 1 protein OS=Cucumis melo OX=3656 GN=LOC103500695 P... [more]
Match NameE-valueIdentityDescription
AT3G13840.11.3e-12648.98GRAS family transcription factor [more]
AT3G49950.12.4e-3528.78GRAS family transcription factor [more]
AT4G37650.11.7e-3328.68GRAS family transcription factor [more]
AT5G66770.12.3e-2226.92GRAS family transcription factor [more]
AT3G03450.15.1e-2223.97RGA-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005202Transcription factor GRASPFAMPF03514GRAScoord: 156..536
e-value: 2.1E-74
score: 250.8
IPR005202Transcription factor GRASPANTHERPTHR31636OSJNBA0084A10.13 PROTEIN-RELATEDcoord: 2..536
IPR005202Transcription factor GRASPROSITEPS50985GRAScoord: 146..536
score: 55.062191
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..118
IPR030015Scarecrow-like protein 29/nodulation signalling pathway 1PANTHERPTHR31636:SF243NODULATION-SIGNALING PATHWAY 1 PROTEIN-LIKEcoord: 2..536

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g02010.1Moc09g02010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042446 hormone biosynthetic process
biological_process GO:2000032 regulation of secondary shoot formation
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding