Moc09g01950 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g01950
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase
Locationchr9: 1635825 .. 1637771 (-)
RNA-Seq ExpressionMoc09g01950
SyntenyMoc09g01950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGTTGCAAAAAGAGTCTCAGTAAAGCTAGAGACATGGAGTGGACAGGTCGACTTTGTGATAGTGCGGATGGACAACTTCGACATAGTATTGGAGATGGAATTTCTGCTGGAACACAAAGTCATCCCCATGCCCCTCGCTAAGTGCCTGGTTGTAACGGGTTCCGATCCCACAGTTGTTCAGACAAGCATCAAACAACCAAGCGGAGTGAAGATGATATCAGCACTCCAGTTAAAGAAAGGTCTCGCTCGTGACGAGCCGACCTTCATGGCCATTCCTGTTGTGGAAGGCGGAAATTCTGAAGAGACAGTTCCCAGAGAAATCCAGCGAGTCTTGGAGGTGTATGCCGATGTAATGCCAGACAACCTGCCCAAAAATCTACCTCCAAGACGGGGGATAGACCATGAGATTGAATTATTATCGGGGGCAAAACCGCCCGCCAAGAACGCCTATCGAATGGCTCCTCCCGAGCTAGCCGAACTGAGGAAACAACTTGATGAACTATTGAATGCTGGATTCATCCGCCCGGCTAAGGCTCCCTATGGGGCCCCAGTCCTTTTTCAGAAGAAGAAAGACGGAAGTCTCCGCCTATGCATCGACTATCGGGCATTGAACAAACTCACAGTCCGCAACAAGTACCTTTTGCCCATCATCACAGACCTGTTCGATCAACTTCATGGGGCAAAGTGCTTCTCAAAGCTGGATCTGCGATCTGGATACTATCAGGTGCGCATCGCAGAAGGAGATGAACCGAAGACGACCTGCGTCACTCGGTATGGGGCCTTTGAATTTCTTGTCATGCCCTTCGGCCTTACGAATGCTCCTGCCACCTTCTGTACCTTAATGAACCAAGTGTTTCATGAATATCTCGACAAATTCGTGGTGGTTTACCTAGATGATATAGTCGTCTACAGTCCAACCATGGAAGAACACTAGGTACATTTACAGCTTGTTTTCGAGAAGCTCAAACAGAACTAGTTGTATGTTAAACGAGAAAAGTGTTCGTTTGCCCAAGAACGCATCAATTTCTTGGGTCATGTGATAGAATGTGGTCGAATAGGCATGGAGGATGGAAAGGTCAAAGCCATTCAAGAGTGGAAGATTCCGACGTCCATCACAGAACTACGTTCATTCCTCGGATTAGCCAATTACTATAGGCGATTCGTGGAAGGATTCTCGAAGCGGACCGGGCCCTTAACGGAATTACTGAAGAAGAACCAGAAGTGGAATTGGACCCCAGAATGTCACGCCGCATTTGAGAGTTTGAAAAAGGTGATGATGGAGGGGCCGGTGTTGGGAATTGCAGACGTCACACGACCGTTAGAAGTCGAGACTGATGCGTCATACTTCGCCCTGGGAGGAGTGCTTCTCCAAGACGGTCACCCCATTGCATACGAGAGTCGGAAGTTGAATGATGCTGAAAGGAGGTATGTCGCCTCCGTGAAAGAGATGTTAGCAGTAGTCTACTGCTTGAGGGCCTGGAGGCAATATCTCCTAAGGGCCAAGTTCGTTGTCAAGACCGACAACAGCTCAGTCTGTCACTTCTTCAACGAACCGAAGTTGTTGTCCAAGCAAGCTAGGTGGCAAGAGTACCTTGCCGAGTTCGATTTTCAGTTCGAACATAAGCCCCGTCGAGCAAATCAGGCGGCAGATGCCCTTAGCAGAAAGAGTGAACATGCGGCCCTGTGCATGTTAGCTTACCTGAAAGCGAGCAAGCTCACTGGATCCATTCGTGAAGCCATCAGAGAGAACTTACAGAACGACCTAGCTGCCCAAGCGATAATTCAGTTGGCCAACGAAGGGAAGACTAGACAGTTTTGGGTGGAGAATGACCTCCTCTTCACCAAAGGTAACCGTTTGTATGTTCCCCGTTCTGGGAACCTAAGGAAGCTCCTACTTGGAGAATGTCACGACACGATGTGGGCAGGCCATGCTGGATGGTAG

mRNA sequence

ATGGGAGTTGCAAAAAGAGTCTCAGTAAAGCTAGAGACATGGAGTGGACAGGTCGACTTTGTGATAGTGCGGATGGACAACTTCGACATAGTATTGGAGATGGAATTTCTGCTGGAACACAAAGTCATCCCCATGCCCCTCGCTAAGTGCCTGGTTGTAACGGGTTCCGATCCCACAGTTGTTCAGACAAGCATCAAACAACCAAGCGGAGTGAAGATGATATCAGCACTCCAGTTAAAGAAAGGTCTCGCTCGTGACGAGCCGACCTTCATGGCCATTCCTGTTGTGGAAGGCGGAAATTCTGAAGAGACAGTTCCCAGAGAAATCCAGCGAGTCTTGGAGGTGTATGCCGATAACCAGAAGTGGAATTGGACCCCAGAATGTCACGCCGCATTTGAGAGTTTGAAAAAGGTGATGATGGAGGGGCCGGTGTTGGGAATTGCAGACGTCACACGACCGTTAGAAGTCGAGACTGATGCGTCATACTTCGCCCTGGGAGGAGTGCTTCTCCAAGACGGTCACCCCATTGCATACGAGAGTCGGAAGTTGAATGATGCTGAAAGGAGGTATGTCGCCTCCGTGAAAGAGATGTTAGCAGTAGTCTACTGCTTGAGGGCCTGGAGGCAATATCTCCTAAGGGCCAAGTTCGTTGTCAAGACCGACAACAGCTCAGTCTGTCACTTCTTCAACGAACCGAAGTTGTTGTCCAAGCAAGCTAGGTGGCAAGAGTACCTTGCCGAGTTCGATTTTCAGTTCGAACATAAGCCCCGTCGAGCAAATCAGGCGGCAGATGCCCTTAGCAGAAAGAGTGAACATGCGGCCCTGTGCATGTTAGCTTACCTGAAAGCGAGCAAGCTCACTGGATCCATTCGTGAAGCCATCAGAGAGAACTTACAGAACGACCTAGCTGCCCAAGCGATAATTCAGTTGGCCAACGAAGGGAAGACTAGACAGTTTTGGGTGGAGAATGACCTCCTCTTCACCAAAGGTAACCGTTTGTATGTTCCCCGTTCTGGGAACCTAAGGAAGCTCCTACTTGGAGAATGTCACGACACGATGTGGGCAGGCCATGCTGGATGGTAG

Coding sequence (CDS)

ATGGGAGTTGCAAAAAGAGTCTCAGTAAAGCTAGAGACATGGAGTGGACAGGTCGACTTTGTGATAGTGCGGATGGACAACTTCGACATAGTATTGGAGATGGAATTTCTGCTGGAACACAAAGTCATCCCCATGCCCCTCGCTAAGTGCCTGGTTGTAACGGGTTCCGATCCCACAGTTGTTCAGACAAGCATCAAACAACCAAGCGGAGTGAAGATGATATCAGCACTCCAGTTAAAGAAAGGTCTCGCTCGTGACGAGCCGACCTTCATGGCCATTCCTGTTGTGGAAGGCGGAAATTCTGAAGAGACAGTTCCCAGAGAAATCCAGCGAGTCTTGGAGGTGTATGCCGATAACCAGAAGTGGAATTGGACCCCAGAATGTCACGCCGCATTTGAGAGTTTGAAAAAGGTGATGATGGAGGGGCCGGTGTTGGGAATTGCAGACGTCACACGACCGTTAGAAGTCGAGACTGATGCGTCATACTTCGCCCTGGGAGGAGTGCTTCTCCAAGACGGTCACCCCATTGCATACGAGAGTCGGAAGTTGAATGATGCTGAAAGGAGGTATGTCGCCTCCGTGAAAGAGATGTTAGCAGTAGTCTACTGCTTGAGGGCCTGGAGGCAATATCTCCTAAGGGCCAAGTTCGTTGTCAAGACCGACAACAGCTCAGTCTGTCACTTCTTCAACGAACCGAAGTTGTTGTCCAAGCAAGCTAGGTGGCAAGAGTACCTTGCCGAGTTCGATTTTCAGTTCGAACATAAGCCCCGTCGAGCAAATCAGGCGGCAGATGCCCTTAGCAGAAAGAGTGAACATGCGGCCCTGTGCATGTTAGCTTACCTGAAAGCGAGCAAGCTCACTGGATCCATTCGTGAAGCCATCAGAGAGAACTTACAGAACGACCTAGCTGCCCAAGCGATAATTCAGTTGGCCAACGAAGGGAAGACTAGACAGTTTTGGGTGGAGAATGACCTCCTCTTCACCAAAGGTAACCGTTTGTATGTTCCCCGTTCTGGGAACCTAAGGAAGCTCCTACTTGGAGAATGTCACGACACGATGTGGGCAGGCCATGCTGGATGGTAG

Protein sequence

MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTVVQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYADNQKWNWTPECHAAFESLKKVMMEGPVLGIADVTRPLEVETDASYFALGGVLLQDGHPIAYESRKLNDAERRYVASVKEMLAVVYCLRAWRQYLLRAKFVVKTDNSSVCHFFNEPKLLSKQARWQEYLAEFDFQFEHKPRRANQAADALSRKSEHAALCMLAYLKASKLTGSIREAIRENLQNDLAAQAIIQLANEGKTRQFWVENDLLFTKGNRLYVPRSGNLRKLLLGECHDTMWAGHAGW
Homology
BLAST of Moc09g01950 vs. NCBI nr
Match: XP_022155185.1 (uncharacterized protein LOC111022320 [Momordica charantia])

HSP 1 Score: 550.4 bits (1417), Expect = 1.1e-152
Identity = 326/606 (53.80%), Postives = 336/606 (55.45%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            MGVAKRVSVKL TWSG VDFVIVRMD+FD+VL ++FLLEHKVIPMPLAKCLVVT SDP V
Sbjct: 481  MGVAKRVSVKLGTWSGLVDFVIVRMDDFDVVLGIKFLLEHKVIPMPLAKCLVVTSSDPIV 540

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQTSIKQPSGVKMISALQLKKG+A+DEPTFMAIPV EG NSEE VPREIQRVLE YAD  
Sbjct: 541  VQTSIKQPSGVKMISALQLKKGIAQDEPTFMAIPVAEGRNSEELVPREIQRVLEAYADVM 600

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 601  PDNLPKNLPPRPGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 660

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 661  PYGAPVLFQKKKDXSLRLCIDYRALNKLXVRNKXPLPIITDLFDQLHGAKYFSKLDLRSG 720

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 721  YYQVFHEYLDKFVVVYLDDIVVYSPTMEEHQVHLQLVFEKLKQNQLYVKREKCSFAQERI 780

Query: 301  ------------------------------------------------------------ 360
                                                                        
Sbjct: 781  SFLGHVIECGRIGMEDGKVKAIQEWKIPTSITELRSFLGLANYYRRFVEGFSKRTGPLTD 840

BLAST of Moc09g01950 vs. NCBI nr
Match: TYK08407.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 438.0 bits (1125), Expect = 8.1e-119
Identity = 257/607 (42.34%), Postives = 304/607 (50.08%), Query Frame = 0

Query: 1   MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
           +G+ KR +++L  WSG VDFV+V++D+FD+VL MEFLLEH+VI MPLAKCLV+TGS P+V
Sbjct: 41  IGLVKRTTIRLGGWSGLVDFVVVKIDDFDVVLGMEFLLEHQVILMPLAKCLVITGSTPSV 100

Query: 61  VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
           VQ  ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 101 VQVDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVM 160

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 161 PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRLAKA 220

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 221 PYGALVLFQKKKDGNLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCT 280

Query: 241 --------------------------------------------NQ-------------- 300
                                                       NQ              
Sbjct: 281 LMNQVFHEYLDKFVVVYLDDIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQER 340

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 341 INFLGHVIECGRIGMEEGKIAAIRDWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLT 400

BLAST of Moc09g01950 vs. NCBI nr
Match: TYK07954.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 427.2 bits (1097), Expect = 1.4e-115
Identity = 260/648 (40.12%), Postives = 305/648 (47.07%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            +G+ KR  ++L  WSG VDFV+V+MD+FD+VL MEFLLEH+VIPMPLAKCLV+TG  P+V
Sbjct: 491  IGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSV 550

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQT ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 551  VQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLNSSENSGETVPKEIVRVLEKYRDVM 610

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 611  PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 670

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 671  PYGAPVLFQKKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSG 730

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 731  YYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLD 790

Query: 301  -------------------------NQ--------------------------------- 360
                                     NQ                                 
Sbjct: 791  DIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGK 850

BLAST of Moc09g01950 vs. NCBI nr
Match: KAA0036830.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 427.2 bits (1097), Expect = 1.4e-115
Identity = 260/648 (40.12%), Postives = 304/648 (46.91%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            +G+ KR  ++L  WSG VDFV+V+MD+FD+VL MEFLLEH+VIPMPLAKCLV+TG  P+V
Sbjct: 521  IGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSV 580

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQT ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 581  VQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVM 640

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 641  PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 700

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 701  PYGAPVLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSG 760

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 761  YYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLD 820

Query: 301  -------------------------NQ--------------------------------- 360
                                     NQ                                 
Sbjct: 821  DIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGK 880

BLAST of Moc09g01950 vs. NCBI nr
Match: KAA0067557.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 426.8 bits (1096), Expect = 1.9e-115
Identity = 259/648 (39.97%), Postives = 304/648 (46.91%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            +G+ KR  ++L  WSG VDFV+V+MD+FD+VL MEFLLEH+VIPMPLAKCLV+TG  P+V
Sbjct: 517  IGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSV 576

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQT ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 577  VQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVM 636

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 637  PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 696

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 697  PYGAPVLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSG 756

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 757  YYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLD 816

Query: 301  -------------------------NQ--------------------------------- 360
                                     NQ                                 
Sbjct: 817  DIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGK 876

BLAST of Moc09g01950 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 6.0e-24
Identity = 59/143 (41.26%), Postives = 85/143 (59.44%), Query Frame = 0

Query: 126 PECHAAFESLKKVMMEGPVLGIADVTRPLEVETDASYFALGGVLLQDGHPIAYESRKLND 185
           PE  +AF+ LK ++ E P+L + D T+   + TDAS  ALG VL QDGHP++Y SR LN+
Sbjct: 481 PEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLNE 540

Query: 186 AERRYVASVKEMLAVVYCLRAWRQYLLRAKFVVKTDNSSVCHFFNEPKLLSKQARWQEYL 245
            E  Y    KE+LA+V+  + +R YLL   F + +D+  +   +      SK  RW+  L
Sbjct: 541 HEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWLYRMKDPNSKLTRWRVKL 600

Query: 246 AEFDFQFEHKPRRANQAADALSR 269
           +EFDF  ++   + N  ADALSR
Sbjct: 601 SEFDFDIKYIKGKENCVADALSR 623

BLAST of Moc09g01950 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.3e-22
Identity = 70/255 (27.45%), Postives = 125/255 (49.02%), Query Frame = 0

Query: 121 KWNWTPECHAAFESLKKVMMEGPVLGIADVTRPLEVETDASYFALGGVLLQDG-----HP 180
           +W WTP    A E++K+ ++  PVL   D ++ + +ETDAS  A+G VL Q       +P
Sbjct: 675 RWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYP 734

Query: 181 IAYESRKLNDAERRYVASVKEMLAVVYCLRAWRQYLLRA--KFVVKTDNSS-VCHFFNEP 240
           + Y S K++ A+  Y  S KEMLA++  L+ WR YL      F + TD+ + +    NE 
Sbjct: 735 VGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNES 794

Query: 241 KLLSKQ-ARWQEYLAEFDFQFEHKPRRANQAADALSRKSEHAALC-------MLAYLKAS 300
           +  +K+ ARWQ +L +F+F+  ++P  AN  ADALSR  +             + ++   
Sbjct: 795 EPENKRLARWQLFLQDFNFEINYRPGSANHIADALSRIVDETEPIPKDSEDNSINFVNQI 854

Query: 301 KLTGSIREAIRENLQNDLAAQAIIQLANEGKTRQFWVENDLLFTKGNRLYVPRSGNLRKL 360
            +T   +  +     ND     ++   ++       +++ LL    +++ +P    L + 
Sbjct: 855 SITDDFKNQVVTEYTNDTKLLNLLNNEDKRVEENIQLKDGLLINSKDQILLPNDTQLTRT 914

BLAST of Moc09g01950 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.3e-22
Identity = 70/255 (27.45%), Postives = 125/255 (49.02%), Query Frame = 0

Query: 121 KWNWTPECHAAFESLKKVMMEGPVLGIADVTRPLEVETDASYFALGGVLLQDG-----HP 180
           +W WTP    A E++K+ ++  PVL   D ++ + +ETDAS  A+G VL Q       +P
Sbjct: 675 RWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYP 734

Query: 181 IAYESRKLNDAERRYVASVKEMLAVVYCLRAWRQYLLRA--KFVVKTDNSS-VCHFFNEP 240
           + Y S K++ A+  Y  S KEMLA++  L+ WR YL      F + TD+ + +    NE 
Sbjct: 735 VGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNES 794

Query: 241 KLLSKQ-ARWQEYLAEFDFQFEHKPRRANQAADALSRKSEHAALC-------MLAYLKAS 300
           +  +K+ ARWQ +L +F+F+  ++P  AN  ADALSR  +             + ++   
Sbjct: 795 EPENKRLARWQLFLQDFNFEINYRPGSANHIADALSRIVDETEPIPKDSEDNSINFVNQI 854

Query: 301 KLTGSIREAIRENLQNDLAAQAIIQLANEGKTRQFWVENDLLFTKGNRLYVPRSGNLRKL 360
            +T   +  +     ND     ++   ++       +++ LL    +++ +P    L + 
Sbjct: 855 SITDDFKNQVVTEYTNDTKLLNLLNNEDKRVEENIQLKDGLLINSKDQILLPNDTQLTRT 914

BLAST of Moc09g01950 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.3e-22
Identity = 70/255 (27.45%), Postives = 125/255 (49.02%), Query Frame = 0

Query: 121 KWNWTPECHAAFESLKKVMMEGPVLGIADVTRPLEVETDASYFALGGVLLQDG-----HP 180
           +W WTP    A E++K+ ++  PVL   D ++ + +ETDAS  A+G VL Q       +P
Sbjct: 675 RWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYP 734

Query: 181 IAYESRKLNDAERRYVASVKEMLAVVYCLRAWRQYLLRA--KFVVKTDNSS-VCHFFNEP 240
           + Y S K++ A+  Y  S KEMLA++  L+ WR YL      F + TD+ + +    NE 
Sbjct: 735 VGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNES 794

Query: 241 KLLSKQ-ARWQEYLAEFDFQFEHKPRRANQAADALSRKSEHAALC-------MLAYLKAS 300
           +  +K+ ARWQ +L +F+F+  ++P  AN  ADALSR  +             + ++   
Sbjct: 795 EPENKRLARWQLFLQDFNFEINYRPGSANHIADALSRIVDETEPIPKDSEDNSINFVNQI 854

Query: 301 KLTGSIREAIRENLQNDLAAQAIIQLANEGKTRQFWVENDLLFTKGNRLYVPRSGNLRKL 360
            +T   +  +     ND     ++   ++       +++ LL    +++ +P    L + 
Sbjct: 855 SITDDFKNQVVTEYTNDTKLLNLLNNEDKRVEENIQLKDGLLINSKDQILLPNDTQLTRT 914

BLAST of Moc09g01950 vs. ExPASy Swiss-Prot
Match: P0CT35 (Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-2 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.3e-22
Identity = 70/255 (27.45%), Postives = 125/255 (49.02%), Query Frame = 0

Query: 121 KWNWTPECHAAFESLKKVMMEGPVLGIADVTRPLEVETDASYFALGGVLLQDG-----HP 180
           +W WTP    A E++K+ ++  PVL   D ++ + +ETDAS  A+G VL Q       +P
Sbjct: 675 RWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAVLSQKHDDDKYYP 734

Query: 181 IAYESRKLNDAERRYVASVKEMLAVVYCLRAWRQYLLRA--KFVVKTDNSS-VCHFFNEP 240
           + Y S K++ A+  Y  S KEMLA++  L+ WR YL      F + TD+ + +    NE 
Sbjct: 735 VGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYLESTIEPFKILTDHRNLIGRITNES 794

Query: 241 KLLSKQ-ARWQEYLAEFDFQFEHKPRRANQAADALSRKSEHAALC-------MLAYLKAS 300
           +  +K+ ARWQ +L +F+F+  ++P  AN  ADALSR  +             + ++   
Sbjct: 795 EPENKRLARWQLFLQDFNFEINYRPGSANHIADALSRIVDETEPIPKDSEDNSINFVNQI 854

Query: 301 KLTGSIREAIRENLQNDLAAQAIIQLANEGKTRQFWVENDLLFTKGNRLYVPRSGNLRKL 360
            +T   +  +     ND     ++   ++       +++ LL    +++ +P    L + 
Sbjct: 855 SITDDFKNQVVTEYTNDTKLLNLLNNEDKRVEENIQLKDGLLINSKDQILLPNDTQLTRT 914

BLAST of Moc09g01950 vs. ExPASy TrEMBL
Match: A0A6J1DLQ6 (uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022320 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 5.4e-153
Identity = 326/606 (53.80%), Postives = 336/606 (55.45%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            MGVAKRVSVKL TWSG VDFVIVRMD+FD+VL ++FLLEHKVIPMPLAKCLVVT SDP V
Sbjct: 481  MGVAKRVSVKLGTWSGLVDFVIVRMDDFDVVLGIKFLLEHKVIPMPLAKCLVVTSSDPIV 540

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQTSIKQPSGVKMISALQLKKG+A+DEPTFMAIPV EG NSEE VPREIQRVLE YAD  
Sbjct: 541  VQTSIKQPSGVKMISALQLKKGIAQDEPTFMAIPVAEGRNSEELVPREIQRVLEAYADVM 600

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 601  PDNLPKNLPPRPGIDHEIELLPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 660

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 661  PYGAPVLFQKKKDXSLRLCIDYRALNKLXVRNKXPLPIITDLFDQLHGAKYFSKLDLRSG 720

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 721  YYQVFHEYLDKFVVVYLDDIVVYSPTMEEHQVHLQLVFEKLKQNQLYVKREKCSFAQERI 780

Query: 301  ------------------------------------------------------------ 360
                                                                        
Sbjct: 781  SFLGHVIECGRIGMEDGKVKAIQEWKIPTSITELRSFLGLANYYRRFVEGFSKRTGPLTD 840

BLAST of Moc09g01950 vs. ExPASy TrEMBL
Match: A0A5D3C8W7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00190 PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 3.9e-119
Identity = 257/607 (42.34%), Postives = 304/607 (50.08%), Query Frame = 0

Query: 1   MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
           +G+ KR +++L  WSG VDFV+V++D+FD+VL MEFLLEH+VI MPLAKCLV+TGS P+V
Sbjct: 41  IGLVKRTTIRLGGWSGLVDFVVVKIDDFDVVLGMEFLLEHQVILMPLAKCLVITGSTPSV 100

Query: 61  VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
           VQ  ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 101 VQVDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVM 160

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 161 PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRLAKA 220

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 221 PYGALVLFQKKKDGNLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCT 280

Query: 241 --------------------------------------------NQ-------------- 300
                                                       NQ              
Sbjct: 281 LMNQVFHEYLDKFVVVYLDDIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQER 340

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 341 INFLGHVIECGRIGMEEGKIAAIRDWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLT 400

BLAST of Moc09g01950 vs. ExPASy TrEMBL
Match: A0A5A7T0E2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20G001310 PE=4 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 6.9e-116
Identity = 260/648 (40.12%), Postives = 304/648 (46.91%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            +G+ KR  ++L  WSG VDFV+V+MD+FD+VL MEFLLEH+VIPMPLAKCLV+TG  P+V
Sbjct: 521  IGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSV 580

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQT ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 581  VQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVM 640

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 641  PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 700

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 701  PYGAPVLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSG 760

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 761  YYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLD 820

Query: 301  -------------------------NQ--------------------------------- 360
                                     NQ                                 
Sbjct: 821  DIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGK 880

BLAST of Moc09g01950 vs. ExPASy TrEMBL
Match: A0A5D3C9P8 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00750 PE=4 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 6.9e-116
Identity = 260/648 (40.12%), Postives = 305/648 (47.07%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            +G+ KR  ++L  WSG VDFV+V+MD+FD+VL MEFLLEH+VIPMPLAKCLV+TG  P+V
Sbjct: 491  IGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSV 550

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQT ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 551  VQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLNSSENSGETVPKEIVRVLEKYRDVM 610

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 611  PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 670

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 671  PYGAPVLFQKKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSG 730

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 731  YYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLD 790

Query: 301  -------------------------NQ--------------------------------- 360
                                     NQ                                 
Sbjct: 791  DIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGK 850

BLAST of Moc09g01950 vs. ExPASy TrEMBL
Match: A0A5D3C4R1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001990 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 9.1e-116
Identity = 259/648 (39.97%), Postives = 304/648 (46.91%), Query Frame = 0

Query: 1    MGVAKRVSVKLETWSGQVDFVIVRMDNFDIVLEMEFLLEHKVIPMPLAKCLVVTGSDPTV 60
            +G+ KR  ++L  WSG VDFV+V+MD+FD+VL MEFLLEH+VIPMPLAKCLV+TG  P+V
Sbjct: 521  IGLVKRTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSV 580

Query: 61   VQTSIKQPSGVKMISALQLKKGLARDEPTFMAIPVVEGGNSEETVPREIQRVLEVYAD-- 120
            VQT ++QP G+KMISA+QLKKGL+RDEPTFMAIP+    NS ETVP+EI RVLE Y D  
Sbjct: 581  VQTDLRQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVM 640

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 641  PDSLPKSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKA 700

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 701  PYGAPVLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSG 760

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 761  YYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLD 820

Query: 301  -------------------------NQ--------------------------------- 360
                                     NQ                                 
Sbjct: 821  DIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGK 880

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155185.11.1e-15253.80uncharacterized protein LOC111022320 [Momordica charantia][more]
TYK08407.18.1e-11942.34reverse transcriptase [Cucumis melo var. makuwa][more]
TYK07954.11.4e-11540.12reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0036830.11.4e-11540.12reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0067557.11.9e-11539.97reverse transcriptase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P043236.0e-2441.26Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
Q9UR074.3e-2227.45Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT414.3e-2227.45Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT344.3e-2227.45Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P0CT354.3e-2227.45Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
A0A6J1DLQ65.4e-15353.80uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5D3C8W73.9e-11942.34Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold65... [more]
A0A5A7T0E26.9e-11640.12Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20... [more]
A0A5D3C9P86.9e-11640.12Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26... [more]
A0A5D3C4R19.1e-11639.97Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.20.370coord: 149..220
e-value: 2.3E-7
score: 32.7
NoneNo IPR availablePANTHERPTHR45835YALI0A06105Pcoord: 85..297
NoneNo IPR availableCDDcd09274RNase_HI_RT_Ty3coord: 155..269
e-value: 9.0377E-52
score: 166.129
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 124..218
e-value: 1.2E-29
score: 102.2
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 115..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g01950.1Moc09g01950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity