Moc09g00340 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g00340
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	S-protein homolog
Location	chr9: 400575 .. 404396 (-)
RNA-Seq Expression	Moc09g00340
Synteny	Moc09g00340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGGGCAAAGTGTGTATATATATTTATTCATGTCCAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAATATATCCAACAAATAGGTTTGTGGCTGTTTTGTTGCTGGTATCGGCAGCTCTTGTGGGGGCTCAGACGAAGCCGTCGGGGTCACCGTCGTTGTTGGTGACTCAAAGATGGCATGTTCACGTGGTTAATAATCTAAACAACAATTTCTTGGATGTGCACTGCAAGTCGAAAGACGATGATTTGGGCTATCAACGCTTGGTTCGTGGAGCTGACTTCCAATGGAGCTTTAAAGTTAACTTTTGGGGAACAACTTTGTTTTGGTGTAACTTACACAAGCCAGGTGCCTATGTCACGTTTGAAGTATTTTGGCCTGAATCAAGGAATGCATGGCTTCGTAATAGGTGCCATAATGGAACTGAAGGACATTGCATTTGGACGGCTAAAGATGATGGAATTTACTTGAGAAATATTCCGACTAATTCTGAAGAGTTGATTCATAAGTGGATCAGTTTGAAATAGTGAAACTTAATTCATTTTGTCATCAACTCATGAAAAATATGCATGCATGTCCTAGTTGTTTTTCTAAATAAGGCTCTATTTGGTCATGTTAGCATTTCCCTTATTTTCCTTTCCTTAGTTTCTTTGTCTCTTGTATTTTGTCTTTAATTAACAAAGAGAATAAATATGAGTGTTGTATAATATCTATGGATTCTTGCTTTTATTTTTATTTTTTTTTACGGAAATGCATTTAATAACGGCCGGTAAAAGAAAAATTACATTTGAAAGATCTAATTAGCATGTTAAAATATTATTTTATTCACAAAATCTCAATATTTTTTATATTAGAAAATAAAACAATTAAATGCATGTAATTGTAACGATAATTAAAAGACAATATAGTAAAGAGACGATACAAAGACGTTTTTAACGTGGTTCCATTTCTCAACCTACCCCACCAGACCACACCTAAAGAATAAGATTAACAATAAAAAATTAGTACAATTTACAATCAATATTAACTTATACGTAAAAGAACTCCTTCTTAAATCTGTCGCGATACTACTTGATCGACTTCTCCTTAGACTAAATCTTTTCAAATACAAGCTCCCATGTATGTTGTAGAACACTGCTTAAACTCCCTTTAACGGTAGATGCATCTTCTCCCAAGACTTTATAAAAATCTCACAAAAGAAAAACAACTCACATAATTTGTACTTATCGAATTACATAAAAAAGATTAAAATAAATAAATAAATAAAATCTCTCAAAATATTACCCCTTAAACAATTCTTGAACAATTAATTCAACATTTCTATGTTCCACTTAAGATTACACAATGAATAGATAAGAAAAAATTGACGTGGGAAGGAAATTGAATTTAACACAATTCTAATTAAACCACAAATCTCTAGATACAATTAAATCACAATTCTCGCACAATCCGATATAGTAAAACTTTCCATATTGTACTATGTCATCCATATTTCCAAAAAAATAAACTAGTCGTGGAAACATTTAGAGCTCAAATTCGAATTCGAAAACACAAACAAGTAATTGAATTAGACACATAAAATAAATAAAGGAAATGACTAAAATTCCTTTCAACATTCAATGGCATTAATTGGAAAAACGCTATCTCTCGACTTTCCAGTATCATGGTTGTTAACGATCTTCGCTTTCACAATCTCGCCATAGGAAGATAAAGCCTCTTTTAAGGCATCATTATCAATGGTGCAAACGAGGCCACCATTGAAGATGGAACTCAACATTTCAAAAAGCCATTGAAATCAAAATCAAAACCCTAGGAAGGGGCAAAAGGGGAAGAAACCATCAAACCGTACCAATCAAGTGAGGTTTGGATTCCTCTAACAGTTCGATTCGGTTTCTATTTTCACATAAAACATGCAGTTTGCTTTTGATTCAACTCTTAAATCGAGCTTAACCAATCCGTGAACATCCCAACTTGATTCATTTAATAGAGAGTAATTTACAACAACCACTGCAGACTTCGTCTAGCCTTTCCCTTGTCAAAGAGGACCGATGAGGTCCACTCTGATCCCCATTGAGCAAAAGACCAAGGATCATTATGTTCGTTAGTGGCTCAAGTGGTTGTCTGGGTACTGCTCTAAATCACTAACATCTGTCGCATCTCTTTGTAAACTGCATGACATCGTTTAACGAGGTTGGCCAATAATACCCTTGGCAAATAATTTTGTGACTTAAGGACCGTGCTTCAAAGTTATTATTGCATACTCGTTCATGTATCACTATCATGACATAGTTAGCTTCATCAAGAGCGACCCACTTTAAGAGTGGCAAGAGTACCTTATCTTGTATGGGTTTCCATCCTTAAGTGTATGTACCGAGCTACCTCGCCGTTTGAGTTGTCGGGCTTGCATTTTCTCTAGTGGTAGAGTTCCATCCTTCAAATACATGACTAGTGGATTCATCCAATTATGCTCGAACTATTCTGATCCATCGACCTCTATCACATCTTGTACTTTTATGGTCAGCTCGGTAAGGATTTTAACTTATAGTTCTTCTCAAATCTGTCTCGTAGGCTCCAGCAAATCGAACCAAGGAATTCGTTCTTAACATTCTAAAAACATTGGATTTGTCTGATTTTGAATTGGAAAACTATGACAAATAGGTCTTTGACCTTTACTTAGTAATTTTCCGTTCGTAGCTCTTTCGCATTTTGGTTCACAATCAATTGTGAATCACTAGGAATAAGAAGATTGGTGATGTCCAATATATTTCGTCATTCTCAATCCTACTAGTAGAGTTTCATATTCAACTTTGTTATTAGAATCATGAAAACGGAATCGAAGCGCATACTCAAATCATTCCCCATCAATGAGACGAATAGCACCCATGCACCACATCCTCTAATTTTCAAGGATCCAGCTACATTGAGTGTTTCAATTTGGTTTTTCCTTGAGGTATGGAATGAGTTGAAACTTCACATTGTACATTGGGTATTCCGAGTCTTTTTTGGGCTATAAAATCTGCTATAGCCCAAAAAAGAAAATGAATGCTTTTATATTTGTAGTCTTAGTTGTAGGTTTATCCAACAGGTAACCATGATGTGAATCAAATATTCTATTCGATGAATTAATTTCTTGAAAGGTTAACAAATTCCTGGCTTAATATTGCAATCTCATCATTTACCAAATGAAAAGAAAAAAAAATCTCTTGCATTATACAATTTTAGGTAAGTTAATTTAACTTTCATAATCTCTTTACATTATACGATTCCTTTTCTTGCTTGGGCAAAGTGTGTATATGTATTTATTCATGTACAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAAAATATCCAACAAATAGGTACGTGGTTGTCTTGTTGCTGGTCATGGTGGCTCTTGTGGGTGCATCAGGGTTACCGTTAGGAAGATGGAACGTTCACATTGTTAATAAGTTAAACAATGATGTCTTGGCTGTGCACTGCAAGTCGAAAGACAATGATTTGCGCTATCAACGCTTGGTTCCTGGAGCTGATTTCCATTGGAGCTTTCGAGTGAATGTGTGGGGAACAACCTTATTTTGGTGCAACTTACAAAAGTCAAGTGCCTATGCCTCGTTTGATACATTTTGGCCTGAGTCGAAGAATCAGTGGCTTCGTTATAGGTGCACCGATAGAACCAAGGGAAATTGCATTTGGACGGCCAAAGATGACGGAATTTACTTGAAAAACATTCCTGATAATGTTGAAGAGTTGGTTCATAAGTGGACCAGTTCCTAA

mRNA sequence

ATGCTTGGGCAAAGTGTGTATATATATTTATTCATGTCCAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAATATATCCAACAAATAGGTTTGTGGCTGTTTTGTTGCTGGTATCGGCAGCTCTTGTGGGGGCTCAGACGAAGCCGTCGGGGTCACCGTCGTTGTTGGTGACTCAAAGATGGCATGTTCACGTGGTTAATAATCTAAACAACAATTTCTTGGATGTGCACTGCAAGTCGAAAGACGATGATTTGGGCTATCAACGCTTGGTTCGTGGAGCTGACTTCCAATGGAGCTTTAAAGTTAACTTTTGGGGAACAACTTTGTTTTGGTGTAACTTACACAAGCCAGGTGCCTATGTCACGTTTGAAGTATTTTGGCCTGAATCAAGGAATGCATGGCTTCGTAATAGGTGCCATAATGGAACTGAAGGACATTGCATTTGGACGGCTAAAGATGATGGAATTTACTTGAGAAATATTCCGACTAATTCTGAAGAGTTGATTCATAAGTGGATCAGGTTACCGTTAGGAAGATGGAACGTTCACATTGTTAATAAGTTAAACAATGATGTCTTGGCTGTGCACTGCAAGTCGAAAGACAATGATTTGCGCTATCAACGCTTGGTTCCTGGAGCTGATTTCCATTGGAGCTTTCGAGTGAATGTGTGGGGAACAACCTTATTTTGGTGCAACTTACAAAAGTCAAGTGCCTATGCCTCGTTTGATACATTTTGGCCTGAGTCGAAGAATCAGTGGCTTCGTTATAGGTGCACCGATAGAACCAAGGGAAATTGCATTTGGACGGCCAAAGATGACGGAATTTACTTGAAAAACATTCCTGATAATGTTGAAGAGTTGGTTCATAAGTGGACCAGTTCCTAA

Coding sequence (CDS)

ATGCTTGGGCAAAGTGTGTATATATATTTATTCATGTCCAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAATATATCCAACAAATAGGTTTGTGGCTGTTTTGTTGCTGGTATCGGCAGCTCTTGTGGGGGCTCAGACGAAGCCGTCGGGGTCACCGTCGTTGTTGGTGACTCAAAGATGGCATGTTCACGTGGTTAATAATCTAAACAACAATTTCTTGGATGTGCACTGCAAGTCGAAAGACGATGATTTGGGCTATCAACGCTTGGTTCGTGGAGCTGACTTCCAATGGAGCTTTAAAGTTAACTTTTGGGGAACAACTTTGTTTTGGTGTAACTTACACAAGCCAGGTGCCTATGTCACGTTTGAAGTATTTTGGCCTGAATCAAGGAATGCATGGCTTCGTAATAGGTGCCATAATGGAACTGAAGGACATTGCATTTGGACGGCTAAAGATGATGGAATTTACTTGAGAAATATTCCGACTAATTCTGAAGAGTTGATTCATAAGTGGATCAGGTTACCGTTAGGAAGATGGAACGTTCACATTGTTAATAAGTTAAACAATGATGTCTTGGCTGTGCACTGCAAGTCGAAAGACAATGATTTGCGCTATCAACGCTTGGTTCCTGGAGCTGATTTCCATTGGAGCTTTCGAGTGAATGTGTGGGGAACAACCTTATTTTGGTGCAACTTACAAAAGTCAAGTGCCTATGCCTCGTTTGATACATTTTGGCCTGAGTCGAAGAATCAGTGGCTTCGTTATAGGTGCACCGATAGAACCAAGGGAAATTGCATTTGGACGGCCAAAGATGACGGAATTTACTTGAAAAACATTCCTGATAATGTTGAAGAGTTGGTTCATAAGTGGACCAGTTCCTAA

Protein sequence

MLGQSVYIYLFMSNMNCNKDRETSFVKKMGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIRLPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQKSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS

Homology

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BLAST of Moc09g00340 vs. NCBI nr
Match: XP_022143839.1 (S-protein homolog 1-like [Momordica charantia])

HSP 1 Score: 340.5 bits (872), Expect = 1.5e-89
Identity = 153/154 (99.35%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 29  MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 88
           MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK
Sbjct: 1   MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 60

Query: 89  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 148
           DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN
Sbjct: 61  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 120

Query: 149 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIRL 183
           GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWI L
Sbjct: 121 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISL 154

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BLAST of Moc09g00340 vs. NCBI nr
Match: KAE8646229.1 (hypothetical protein Csa_016318 [Cucumis sativus])

HSP 1 Score: 305.4 bits (781), Expect = 5.3e-79
Identity = 152/296 (51.35%), Postives = 186/296 (62.84%), Query Frame = 0


Query: 41  VLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQRLV-R 100
           VL L +AALVGAQ      PS      W V +VN L++  L+VHC+SK+DDLG   LV R
Sbjct: 8   VLWLATAALVGAQV-----PS------WSVSIVNGLSHLDLNVHCQSKNDDLGNHHLVKR 67

Query: 101 GADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPES-RNAWLRNRCHNGTEGHCIWTA 160
           G  ++W+FK NFWGTTLFWC L K  AYV+FE FWPES  N WLR+RC  G EG CIW A
Sbjct: 68  GDIYKWNFKENFWGTTLFWCKLEKSDAYVSFETFWPESWSNTWLRDRC--GPEGTCIWVA 127

Query: 161 KDDGIYLRNIPTNSEELIHKW--------------------------------IRLPLGR 220
           KD GIYL+N P N +E +HKW                                IRLP  R
Sbjct: 128 KDGGIYLKNNPANRDEFVHKWWIGKKMMMRNVVVLWLMTATLVAEAQISNKVVIRLPPVR 187

Query: 221 WNVHIVNKLNNDVLAVHCKSKDNDLRYQRLV-PGADFHWSFRVNVWGTTLFWCNLQKSSA 280
           + +H+ N L N  ++ HC+SKD+DL  Q LV  G +F W+F+ N W TTLFWC L+KS+A
Sbjct: 188 YFIHVANDLTNQDMSAHCQSKDDDLGIQHLVHRGDEFRWNFKENFWKTTLFWCRLEKSNA 247

Query: 281 YASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS 302
           Y SFD FWPE K+ WLR RC  R  G CIW+A+DDGIYL+N+P   EE+VHKW S+
Sbjct: 248 YVSFDVFWPERKHHWLRDRCGSR--GVCIWSARDDGIYLRNLPAQSEEIVHKWIST 288

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BLAST of Moc09g00340 vs. NCBI nr
Match: XP_022143852.1 (S-protein homolog 74-like [Momordica charantia])

HSP 1 Score: 273.5 bits (698), Expect = 2.2e-69
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0


Query: 182 LPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQ 241
           LPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQ
Sbjct: 27  LPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQ 86

Query: 242 KSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS 301
           KSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS
Sbjct: 87  KSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS 146

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BLAST of Moc09g00340 vs. NCBI nr
Match: KAG7014486.1 (S-protein-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 226.1 bits (575), Expect = 4.1e-55
Identity = 125/326 (38.34%), Postives = 165/326 (50.61%), Query Frame = 0


Query: 37  RFVAVLLLVSAALVGAQTKPSGSPSLLVTQR---WHVHVVNNLNNNFLDVHCKSKDDDLG 96
           R VA L LV    VGAQ +       +   R   +++H+VN+L    + VHCKSKDDDLG
Sbjct: 3   RNVAALFLVMVGFVGAQLEVESEKINVEPPRISSYNIHLVNDLQVLGITVHCKSKDDDLG 62

Query: 97  YQRLV-RGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEG 156
              L  RG D+ +SFK+N W +TLFWC + K  AY++FE FW E  + WLRNRC +G  G
Sbjct: 63  VHNLPHRGDDYHFSFKINVWQSTLFWCRVEKQNAYISFECFWTEIHHRWLRNRCEDGDVG 122

Query: 157 HCIWTAKDDGIYLRNIPTNSEELIHKWIRLP----------------------------L 216
            C W  KDDGIYLR+   N +EL+   I  P                            L
Sbjct: 123 TCTWKFKDDGIYLRHNSANRDELVPGIIYQPKPSRYYVHVVNGLSTLPLTVHCQSKDDDL 182

Query: 217 G--------------------------RWNVHIVNKLNN-DVLAVHCKSKDNDLRYQRLV 276
           G                          RW+V + N LN  + ++VHC+S+D+DL    L 
Sbjct: 183 GIHHLPNRGDDFQWNFKTKGSVFPAFIRWHVTVTNGLNGAEAMSVHCRSQDDDLGLHTLP 242

Query: 277 PGADFHWSFRVNVWGTTLFWCNLQK-----SSAYASFDTFWPESKNQWLRYRCTDRTKGN 299
           P ADF WSF  N + TTLFWC LQK         A+F  FW +++   L  +C  +   N
Sbjct: 243 PTADFSWSFEANFFHTTLFWCRLQKGGGGGGGVEAAFKVFWHDAR---LFEKCGWK---N 302

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BLAST of Moc09g00340 vs. NCBI nr
Match: KAB2636489.1 (hypothetical protein D8674_027023 [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 222.6 bits (566), Expect = 4.5e-54
Identity = 103/258 (39.92%), Postives = 144/258 (55.81%), Query Frame = 0


Query: 81  LDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNA 140
           L  HC+S DDDLG + +  G +F WSF+ N  G+TL+WC++H    + +F+VFWPE  ++
Sbjct: 7   LVAHCESLDDDLGIRDISDGNEFNWSFRENLSGSTLYWCDMHNDHQHASFKVFWPERNHS 66

Query: 141 WLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEE-----------------LIH------ 200
           WLR RC+      C W AKDDGIYLR IP + E+                 + H      
Sbjct: 67  WLRYRCN---YKECFWIAKDDGIYLRVIPESREDGGARRKQAVEDPTRHKTITHSTAKQG 126

Query: 201 ----------------KWIRLPLGRWNVHIVNKLN-NDVLAVHCKSKDNDLRYQRLVPGA 260
                           KW   P  RW+VH+VN L     L  HC+SK++D+  + + PGA
Sbjct: 127 QTLALSTPCLANFHEDKWELRP-ERWHVHVVNNLGARKTLFAHCQSKNDDIGNRTIAPGA 186

Query: 261 DFHWSFRVNVWGTTLFWCNLQKSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDD 299
           +  WSF+ N +GTTL+WC  +    + +FD +W ESK+ WLRYRC  +    C W AKDD
Sbjct: 187 EITWSFKPNFFGTTLYWCYTRTDHEHTAFDVYWEESKHNWLRYRCNYK---ECFWIAKDD 246

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BLAST of Moc09g00340 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.6e-17
Identity = 47/113 (41.59%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V VVN L     L +HCKSK+DDLG   L     F W+F  N   +T FWC ++K   
Sbjct: 41  WQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMNKDNG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKW 180
           ++   VFW +     L +RC  G + +CIWTAK DG+YL N  +  + L  KW
Sbjct: 101 HMNVNVFWDD---VILFHRC--GWK-NCIWTAKTDGLYLWNSASGEDVLSRKW 147

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BLAST of Moc09g00340 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-15
Identity = 44/113 (38.94%), Postives = 60/113 (53.10%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V V N L     L +HCKSK++DLG   L     F W+F  N   +TLFWC + K   
Sbjct: 41  WQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDRFSWNFGENMLHSTLFWCYMSKDDG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKW 180
           ++  +VFW +     L +RC      +C+WTAK+DG+YL N     + L  KW
Sbjct: 101 HMNVKVFWDD---VILFHRC---DWKNCVWTAKNDGLYLWNSAIGEDVLSEKW 147

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BLAST of Moc09g00340 vs. ExPASy Swiss-Prot
Match: F2Q9V4 (S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 6.8e-13
Identity = 44/144 (30.56%), Postives = 66/144 (45.83%), Query Frame = 0


Query: 41  VLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQRLVRG 100
           ++ +V  +L+G +T         +       V+ N N+  L VHCKS+DDD G+  L +G
Sbjct: 4   IIFIVLISLIGCETLQHDGKVFPMKGPLTRVVIYNDNDYLLGVHCKSRDDDHGFHILQKG 63

Query: 101 ADFQWSFKVNFWGTTLFWCNLH----KPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIW 160
             + W F VNF  +TL++C       K G +  ++     S       RC N     C W
Sbjct: 64  GLYGWMFYVNFMNSTLYFCGFSQEQVKKGVFDIYKAVRDSS-------RCRN-----CTW 123

Query: 161 TAKDDGIYLRNIPTNSEELIHKWI 181
            AK+DGIY          L +KW+
Sbjct: 124 EAKEDGIYGYGEIPKKNPLFYKWL 135

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BLAST of Moc09g00340 vs. ExPASy Swiss-Prot
Match: F4JLQ5 (S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-12
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 0


Query: 56  PSGSPSLLVTQRWHVHVVNNLNNNF-LDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGT 115
           PS + S+  T +  V + N+L N   L  HCKSKDDDLG + L  G  + +SF   F+G 
Sbjct: 36  PSSTNSVFPTSKRTVEINNDLGNQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGR 95

Query: 116 TLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEE 175
           TL++C+   P    +F+++  + R++   N+C +     C+W  + +G    N  T   +
Sbjct: 96  TLYFCSFSWPNESHSFDIY-KDHRDSGGDNKCES---DRCVWKIRRNGPCRFNDETKQFD 155

Query: 176 LIHKW 180
           L + W
Sbjct: 156 LCYPW 156

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BLAST of Moc09g00340 vs. ExPASy Swiss-Prot
Match: Q9LW22 (S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-12
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 73  VNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEV 132
           +N  N N L VHCKSK++D+G + L  G    +SFK NFWGTT FWCNL+K   Y  +  
Sbjct: 32  LNPKNKNILKVHCKSKNNDIGVKYLKIGEVMSFSFKTNFWGTTEFWCNLYKGPDYKRY-- 91

Query: 133 FWPESRNAWLRNRCHNGTEGHCIWTAKDDGIY 165
                  A+              W A+DDGIY
Sbjct: 92  ---RGITAYQAIGLFAKDGSSYNWLARDDGIY 118

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BLAST of Moc09g00340 vs. ExPASy TrEMBL
Match: A0A6J1CQI8 (S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013653 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 7.1e-90
Identity = 153/154 (99.35%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 29  MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 88
           MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK
Sbjct: 1   MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 60

Query: 89  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 148
           DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN
Sbjct: 61  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 120

Query: 149 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIRL 183
           GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWI L
Sbjct: 121 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISL 154

[Close]

BLAST of Moc09g00340 vs. ExPASy TrEMBL
Match: A0A6J1CS23 (S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013665 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 1.1e-69
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0


Query: 182 LPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQ 241
           LPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQ
Sbjct: 27  LPLGRWNVHIVNKLNNDVLAVHCKSKDNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQ 86

Query: 242 KSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS 301
           KSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS
Sbjct: 87  KSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTSS 146

[Close]

BLAST of Moc09g00340 vs. ExPASy TrEMBL
Match: A0A5N5I9W3 (S-protein homolog OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_027023 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 2.2e-54
Identity = 103/258 (39.92%), Postives = 144/258 (55.81%), Query Frame = 0


Query: 81  LDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNA 140
           L  HC+S DDDLG + +  G +F WSF+ N  G+TL+WC++H    + +F+VFWPE  ++
Sbjct: 7   LVAHCESLDDDLGIRDISDGNEFNWSFRENLSGSTLYWCDMHNDHQHASFKVFWPERNHS 66

Query: 141 WLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEE-----------------LIH------ 200
           WLR RC+      C W AKDDGIYLR IP + E+                 + H      
Sbjct: 67  WLRYRCN---YKECFWIAKDDGIYLRVIPESREDGGARRKQAVEDPTRHKTITHSTAKQG 126

Query: 201 ----------------KWIRLPLGRWNVHIVNKLN-NDVLAVHCKSKDNDLRYQRLVPGA 260
                           KW   P  RW+VH+VN L     L  HC+SK++D+  + + PGA
Sbjct: 127 QTLALSTPCLANFHEDKWELRP-ERWHVHVVNNLGARKTLFAHCQSKNDDIGNRTIAPGA 186

Query: 261 DFHWSFRVNVWGTTLFWCNLQKSSAYASFDTFWPESKNQWLRYRCTDRTKGNCIWTAKDD 299
           +  WSF+ N +GTTL+WC  +    + +FD +W ESK+ WLRYRC  +    C W AKDD
Sbjct: 187 EITWSFKPNFFGTTLYWCYTRTDHEHTAFDVYWEESKHNWLRYRCNYK---ECFWIAKDD 246

[Close]

BLAST of Moc09g00340 vs. ExPASy TrEMBL
Match: A0A6J1JMS5 (S-protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111488300 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 2.8e-46
Identity = 91/146 (62.33%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 39  VAVLLLVSAALVGAQTKPSGSPSLLV---TQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQ 98
           V VLLL   A VGAQ      P ++      R++VHV N L+   L VHC+SKDDDLG  
Sbjct: 5   VVVLLLGMVAFVGAQV-----PGIIYQPKPSRYYVHVANGLSTLPLTVHCQSKDDDLGIH 64

Query: 99  RLV-RGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHC 158
            L  RG DFQW+FKVNFWGTTLFWC L +P AYVTFE FWPES+N WLR+RC NGT+G C
Sbjct: 65  HLPNRGDDFQWNFKVNFWGTTLFWCRLERPDAYVTFETFWPESKNTWLRDRCRNGTQGTC 124

Query: 159 IWTAKDDGIYLRNIPTNSEELIHKWI 181
           +W AKDDGIYL N P N++ELIHKWI
Sbjct: 125 LWIAKDDGIYLFNFPANNDELIHKWI 145

[Close]

BLAST of Moc09g00340 vs. ExPASy TrEMBL
Match: A0A5D3DIG9 (S-protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00380 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 4.8e-46
Identity = 90/145 (62.07%), Postives = 105/145 (72.41%), Query Frame = 0


Query: 37  RFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQR 96
           R V VL L    LV  Q      P  L   R+ VHVVN L+   LDVHC+SKDDDLGY R
Sbjct: 4   RNVVVLWLAMMVLVEGQVSKWSKPPPL-PSRYFVHVVNGLSYQSLDVHCQSKDDDLGYHR 63

Query: 97  LVR-GADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCI 156
           LV+ G +FQW+F+ NFWGTTLFWC L KP AYV FE FWPE++N WLR+RC  G +G CI
Sbjct: 64  LVKQGDEFQWNFEENFWGTTLFWCRLEKPDAYVAFESFWPETKNVWLRHRC--GNQGTCI 123

Query: 157 WTAKDDGIYLRNIPTNSEELIHKWI 181
           WTAKDDGIYLRN+PTN++E +HKWI
Sbjct: 124 WTAKDDGIYLRNMPTNADEFVHKWI 145

[Close]

BLAST of Moc09g00340 vs. TAIR 10
Match: AT4G16295.1 (S-protein homologue 1 )

HSP 1 Score: 90.1 bits (222), Expect = 3.2e-18
Identity = 47/113 (41.59%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V VVN L     L +HCKSK+DDLG   L     F W+F  N   +T FWC ++K   
Sbjct: 41  WQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMNKDNG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKW 180
           ++   VFW +     L +RC  G + +CIWTAK DG+YL N  +  + L  KW
Sbjct: 101 HMNVNVFWDD---VILFHRC--GWK-NCIWTAKTDGLYLWNSASGEDVLSRKW 147

[Close]

BLAST of Moc09g00340 vs. TAIR 10
Match: AT4G29035.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 85.5 bits (210), Expect = 8.0e-17
Identity = 44/113 (38.94%), Postives = 60/113 (53.10%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V V N L     L +HCKSK++DLG   L     F W+F  N   +TLFWC + K   
Sbjct: 41  WQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDRFSWNFGENMLHSTLFWCYMSKDDG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKW 180
           ++  +VFW +     L +RC      +C+WTAK+DG+YL N     + L  KW
Sbjct: 101 HMNVKVFWDD---VILFHRC---DWKNCVWTAKNDGLYLWNSAIGEDVLSEKW 147

[Close]

BLAST of Moc09g00340 vs. TAIR 10
Match: AT4G16195.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-13
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 0


Query: 56  PSGSPSLLVTQRWHVHVVNNLNNNF-LDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGT 115
           PS + S+  T +  V + N+L N   L  HCKSKDDDLG + L  G  + +SF   F+G 
Sbjct: 36  PSSTNSVFPTSKRTVEINNDLGNQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGR 95

Query: 116 TLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEE 175
           TL++C+   P    +F+++  + R++   N+C +     C+W  + +G    N  T   +
Sbjct: 96  TLYFCSFSWPNESHSFDIY-KDHRDSGGDNKCES---DRCVWKIRRNGPCRFNDETKQFD 155

Query: 176 LIHKW 180
           L + W
Sbjct: 156 LCYPW 156

[Close]

BLAST of Moc09g00340 vs. TAIR 10
Match: AT3G26880.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-13
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 73  VNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEV 132
           +N  N N L VHCKSK++D+G + L  G    +SFK NFWGTT FWCNL+K   Y  +  
Sbjct: 32  LNPKNKNILKVHCKSKNNDIGVKYLKIGEVMSFSFKTNFWGTTEFWCNLYKGPDYKRY-- 91

Query: 133 FWPESRNAWLRNRCHNGTEGHCIWTAKDDGIY 165
                  A+              W A+DDGIY
Sbjct: 92  ---RGITAYQAIGLFAKDGSSYNWLARDDGIY 118

[Close]

BLAST of Moc09g00340 vs. TAIR 10
Match: AT5G06030.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 69.3 bits (168), Expect = 5.9e-12
Identity = 38/109 (34.86%), Postives = 56/109 (51.38%), Query Frame = 0


Query: 73  VNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEV 132
           + N N+  L +HCKS+DDDLG+  L +G  F W F VNF  +TL +C   +        +
Sbjct: 50  IQNYNDYLLAIHCKSRDDDLGFHILAKGELFGWKFHVNFRYSTLCFCGFSQRQINKGVFI 109

Query: 133 FWPESRNAWLRNRCHNGTEGHCIWTAKDDGIY-LRNIPTNSEELIHKWI 181
            +  SR+ +   RC N     C W A+ DG +   +IPT    L + W+
Sbjct: 110 IYVASRDFY---RCAN-----CTWKAEKDGFHGYGDIPTRG-YLFYNWL 149

The following BLAST results are available for this feature:

BLAST of Moc09g00340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143839.1	1.5e-89	99.35	S-protein homolog 1-like [Momordica charantia]	[more]
KAE8646229.1	5.3e-79	51.35	hypothetical protein Csa_016318 [Cucumis sativus]	[more]
XP_022143852.1	2.2e-69	100.00	S-protein homolog 74-like [Momordica charantia]	[more]
KAG7014486.1	4.1e-55	38.34	S-protein-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAB2636489.1	4.5e-54	39.92	hypothetical protein D8674_027023 [Pyrus ussuriensis x Pyrus communis]	[more]

BLAST of Moc09g00340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JLS0	4.6e-17	41.59	S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1	[more]
Q2HQ46	1.1e-15	38.94	S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1	[more]
F2Q9V4	6.8e-13	30.56	S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2	[more]
F4JLQ5	1.5e-12	32.00	S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1	[more]
Q9LW22	2.0e-12	41.30	S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1	[more]

BLAST of Moc09g00340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQI8	7.1e-90	99.35	S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013653 PE=3 SV=1	[more]
A0A6J1CS23	1.1e-69	100.00	S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013665 PE=3 SV=1	[more]
A0A5N5I9W3	2.2e-54	39.92	S-protein homolog OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_0270...	[more]
A0A6J1JMS5	2.8e-46	62.33	S-protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111488300 PE=3 SV=1	[more]
A0A5D3DIG9	4.8e-46	62.07	S-protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00...	[more]

BLAST of Moc09g00340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16295.1	3.2e-18	41.59	S-protein homologue 1	[more]
AT4G29035.1	8.0e-17	38.94	Plant self-incompatibility protein S1 family	[more]
AT4G16195.1	1.1e-13	32.00	Plant self-incompatibility protein S1 family	[more]
AT3G26880.1	1.4e-13	41.30	Plant self-incompatibility protein S1 family	[more]
AT5G06030.1	5.9e-12	34.86	Plant self-incompatibility protein S1 family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010264	Plant self-incompatibility S1	PFAM	PF05938	Self-incomp_S1	coord: 188..298 e-value: 2.6E-26 score: 92.2 coord: 69..179 e-value: 1.4E-26 score: 93.0
IPR010264	Plant self-incompatibility S1	PANTHER	PTHR31232	FAMILY NOT NAMED	coord: 186..299 coord: 40..180
None	No IPR available	PANTHER	PTHR31232:SF93	BINDING PROTEIN, PUTATIVE-RELATED	coord: 186..299
None	No IPR available	PANTHER	PTHR31232:SF93	BINDING PROTEIN, PUTATIVE-RELATED	coord: 40..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g00340.1	Moc09g00340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060320	rejection of self pollen
cellular_component	GO:0005576	extracellular region