Moc08g45780 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g45780
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionThreonine synthase
Locationchr8: 35095838 .. 35097460 (-)
RNA-Seq ExpressionMoc08g45780
SyntenyMoc08g45780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCTCTTTCTCCCTCTACTCCACACTCTCCCCCAAACACGATTCTCTCTTTCCCCGCCCCGCCGCCCGGAATTCTTTCACCGTCGTCTGTTCCTCCGCCACCTCCGACCCCGCTTCCTCCCCTCTCCTCCACTCATCTCCGCCCATCCGCCTCAACGGCCACCAGCTCGCCGGTGCAAACACCCTAGACCAAGCTCGTCCGCGTCGCTCCATTGTCGGCGACGGCGACCGTAATTTCTCGGCCAAGTACGTGCCGTTTGGTTCTGGCTTTGATTCCCCCGAGTGGTACTCGCTCGACGACATTGTTTATCGGAGCCGCTCCGGCGGGCTTCTCGATGTTCAGCACGACATGGAAGCTTTGAAGAACTTCGACGGAGCGTATTGGCGAAACCTCTTCGATTCGCGCGTGGGGAAGACAACTTGGCCATATGGCTCCGGCGTGTGGAGCAAGAAGGAGTGGGTGCTGCCGGAGATTGATTCCGACGACATCGTCAGCGCCTTCGAGGGAAATTCGAATCTCTTCTGGGCAGAGCGTTTTGGCAAACAGTTTCTAGGTATGAATGACGTGTGGGTCAAACACTGTGGAATTAGTCACACAGGCAGTTTCAAAGACCTTGGAATGACCGTTTTGGTCAGCCAAGTCAATCGTTTGAGGAAAATGAACCGTCCAGTCATTGGTGTTGGCTGTGCCTCAACTGGGGACACCTCCGCCGCGCTATCTGCCTATTGTGCCGCTGCAGGAATTCCATCGATTGTCTTCCTCCCGACCGATAAGATTTCTATCGCACAGTTAGTGCAACCAATTGCAAATGGCGCTTTTGTCTTAAGCATGGACACCGATTTTGACGGTTGCATGAAATTAATCCGAGAAGTCACAGCAGAGCTTCCAATTTACCTAGCAAATTCACTCAACAGTTTAAGATTGGAAGGGCAAAAGACAGCGGCCATTGAAATTCTTCAGCAGTTTGATTGGGAAGTCCCCGATTGGGTTATCATACCAGGGGGCAACTTAGGGAATATATACGCATTTTACAAAGGATTTAAAATGTGCCAAGAATTAGGGCTCGTCGACAAAATTCCGAGGCTGGTTTGTGCTCAAGCGGCAAACGCAAATCCGCTTTACTTATATTACAAATCTGGCTGGAAAGAATTTACGCCATTGAAGGCGAGCACGACGTTTGCATCTGCAATTCAGATTGGAGATCCAGTTTCCATAGACAGAGCTGTGTTTGCTCTGAAGAACTCCGATGGGATTGTGGAAGAAGCGACCGAGGAGGAATTAATGGATGCCACAGCTTTGGCTGATTCGACGGGGATGTTCATTTGCCCGCACACAGGGGTCGCACTAACAGCTCTGATCAAGCTCAGAAACAGAGACGTCATCAGAGCTAATGATAGAACGGTGGTGGTAAGCACAGCCCATGGCCTGAAATTCACTCAATCCAAGATCGATTACCACTCCAGAGCAATCCCGAGCATGGATTGCCGGTTTGCGAACCCACCAGTGGAAGTGAAAGCGGATTTTGGAGCGGTTATGGATGTTCTTAAGGATCATATATTGAGGAGGAGTCGTAAGTCTCAGTTATATGCGAATTTTTCTTTTTCTGAAAGTCAGTAA

mRNA sequence

ATGGCCTCCTCTTTCTCCCTCTACTCCACACTCTCCCCCAAACACGATTCTCTCTTTCCCCGCCCCGCCGCCCGGAATTCTTTCACCGTCGTCTGTTCCTCCGCCACCTCCGACCCCGCTTCCTCCCCTCTCCTCCACTCATCTCCGCCCATCCGCCTCAACGGCCACCAGCTCGCCGGTGCAAACACCCTAGACCAAGCTCGTCCGCGTCGCTCCATTGTCGGCGACGGCGACCGTAATTTCTCGGCCAAGTACGTGCCGTTTGGTTCTGGCTTTGATTCCCCCGAGTGGTACTCGCTCGACGACATTGTTTATCGGAGCCGCTCCGGCGGGCTTCTCGATGTTCAGCACGACATGGAAGCTTTGAAGAACTTCGACGGAGCGTATTGGCGAAACCTCTTCGATTCGCGCGTGGGGAAGACAACTTGGCCATATGGCTCCGGCGTGTGGAGCAAGAAGGAGTGGGTGCTGCCGGAGATTGATTCCGACGACATCGTCAGCGCCTTCGAGGGAAATTCGAATCTCTTCTGGGCAGAGCGTTTTGGCAAACAGTTTCTAGGTATGAATGACGTGTGGGTCAAACACTGTGGAATTAGTCACACAGGCAGTTTCAAAGACCTTGGAATGACCGTTTTGGTCAGCCAAGTCAATCGTTTGAGGAAAATGAACCGTCCAGTCATTGGTGTTGGCTGTGCCTCAACTGGGGACACCTCCGCCGCGCTATCTGCCTATTGTGCCGCTGCAGGAATTCCATCGATTGTCTTCCTCCCGACCGATAAGATTTCTATCGCACAGTTAGTGCAACCAATTGCAAATGGCGCTTTTGTCTTAAGCATGGACACCGATTTTGACGGTTGCATGAAATTAATCCGAGAAGTCACAGCAGAGCTTCCAATTTACCTAGCAAATTCACTCAACAGTTTAAGATTGGAAGGGCAAAAGACAGCGGCCATTGAAATTCTTCAGCAGTTTGATTGGGAAGTCCCCGATTGGGTTATCATACCAGGGGGCAACTTAGGGAATATATACGCATTTTACAAAGGATTTAAAATGTGCCAAGAATTAGGGCTCGTCGACAAAATTCCGAGGCTGGTTTGTGCTCAAGCGGCAAACGCAAATCCGCTTTACTTATATTACAAATCTGGCTGGAAAGAATTTACGCCATTGAAGGCGAGCACGACGTTTGCATCTGCAATTCAGATTGGAGATCCAGTTTCCATAGACAGAGCTGTGTTTGCTCTGAAGAACTCCGATGGGATTGTGGAAGAAGCGACCGAGGAGGAATTAATGGATGCCACAGCTTTGGCTGATTCGACGGGGATGTTCATTTGCCCGCACACAGGGGTCGCACTAACAGCTCTGATCAAGCTCAGAAACAGAGACGTCATCAGAGCTAATGATAGAACGGTGGTGGTAAGCACAGCCCATGGCCTGAAATTCACTCAATCCAAGATCGATTACCACTCCAGAGCAATCCCGAGCATGGATTGCCGGTTTGCGAACCCACCAGTGGAAGTGAAAGCGGATTTTGGAGCGGTTATGGATGTTCTTAAGGATCATATATTGAGGAGGAGTCGTAAGTCTCAGTTATATGCGAATTTTTCTTTTTCTGAAAGTCAGTAA

Coding sequence (CDS)

ATGGCCTCCTCTTTCTCCCTCTACTCCACACTCTCCCCCAAACACGATTCTCTCTTTCCCCGCCCCGCCGCCCGGAATTCTTTCACCGTCGTCTGTTCCTCCGCCACCTCCGACCCCGCTTCCTCCCCTCTCCTCCACTCATCTCCGCCCATCCGCCTCAACGGCCACCAGCTCGCCGGTGCAAACACCCTAGACCAAGCTCGTCCGCGTCGCTCCATTGTCGGCGACGGCGACCGTAATTTCTCGGCCAAGTACGTGCCGTTTGGTTCTGGCTTTGATTCCCCCGAGTGGTACTCGCTCGACGACATTGTTTATCGGAGCCGCTCCGGCGGGCTTCTCGATGTTCAGCACGACATGGAAGCTTTGAAGAACTTCGACGGAGCGTATTGGCGAAACCTCTTCGATTCGCGCGTGGGGAAGACAACTTGGCCATATGGCTCCGGCGTGTGGAGCAAGAAGGAGTGGGTGCTGCCGGAGATTGATTCCGACGACATCGTCAGCGCCTTCGAGGGAAATTCGAATCTCTTCTGGGCAGAGCGTTTTGGCAAACAGTTTCTAGGTATGAATGACGTGTGGGTCAAACACTGTGGAATTAGTCACACAGGCAGTTTCAAAGACCTTGGAATGACCGTTTTGGTCAGCCAAGTCAATCGTTTGAGGAAAATGAACCGTCCAGTCATTGGTGTTGGCTGTGCCTCAACTGGGGACACCTCCGCCGCGCTATCTGCCTATTGTGCCGCTGCAGGAATTCCATCGATTGTCTTCCTCCCGACCGATAAGATTTCTATCGCACAGTTAGTGCAACCAATTGCAAATGGCGCTTTTGTCTTAAGCATGGACACCGATTTTGACGGTTGCATGAAATTAATCCGAGAAGTCACAGCAGAGCTTCCAATTTACCTAGCAAATTCACTCAACAGTTTAAGATTGGAAGGGCAAAAGACAGCGGCCATTGAAATTCTTCAGCAGTTTGATTGGGAAGTCCCCGATTGGGTTATCATACCAGGGGGCAACTTAGGGAATATATACGCATTTTACAAAGGATTTAAAATGTGCCAAGAATTAGGGCTCGTCGACAAAATTCCGAGGCTGGTTTGTGCTCAAGCGGCAAACGCAAATCCGCTTTACTTATATTACAAATCTGGCTGGAAAGAATTTACGCCATTGAAGGCGAGCACGACGTTTGCATCTGCAATTCAGATTGGAGATCCAGTTTCCATAGACAGAGCTGTGTTTGCTCTGAAGAACTCCGATGGGATTGTGGAAGAAGCGACCGAGGAGGAATTAATGGATGCCACAGCTTTGGCTGATTCGACGGGGATGTTCATTTGCCCGCACACAGGGGTCGCACTAACAGCTCTGATCAAGCTCAGAAACAGAGACGTCATCAGAGCTAATGATAGAACGGTGGTGGTAAGCACAGCCCATGGCCTGAAATTCACTCAATCCAAGATCGATTACCACTCCAGAGCAATCCCGAGCATGGATTGCCGGTTTGCGAACCCACCAGTGGAAGTGAAAGCGGATTTTGGAGCGGTTATGGATGTTCTTAAGGATCATATATTGAGGAGGAGTCGTAAGTCTCAGTTATATGCGAATTTTTCTTTTTCTGAAAGTCAGTAA

Protein sequence

MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ
Homology
BLAST of Moc08g45780 vs. NCBI nr
Match: XP_022154650.1 (threonine synthase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG 60
           MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG
Sbjct: 25  MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG 84

Query: 61  ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME 120
           ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME
Sbjct: 85  ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME 144

Query: 121 ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER 180
           ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER
Sbjct: 145 ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER 204

Query: 181 FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA 240
           FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA
Sbjct: 205 FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA 264

Query: 241 LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 300
           LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY
Sbjct: 265 LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 324

Query: 301 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK 360
           LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK
Sbjct: 325 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK 384

Query: 361 IPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGI 420
           IPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGI
Sbjct: 385 IPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGI 444

Query: 421 VEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKF 480
           VEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKF
Sbjct: 445 VEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKF 504

Query: 481 TQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ 540
           TQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ
Sbjct: 505 TQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ 564

BLAST of Moc08g45780 vs. NCBI nr
Match: XP_038891002.1 (threonine synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 934.5 bits (2414), Expect = 4.1e-268
Identity = 466/531 (87.76%), Postives = 490/531 (92.28%), Query Frame = 0

Query: 1   MASS-FSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGH 60
           MASS FS  ++ S K DSLF R A RNS   FTVVCSSATSD A+SP+LHSSPP+   GH
Sbjct: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPL---GH 60

Query: 61  QLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQ 120
           Q A AN  D +  R S+  D   NFSAKYVPFGSG+DS EWYSLD+IVYRSRSGGLLDVQ
Sbjct: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120

Query: 121 HDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLF 180
           H+MEALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLF
Sbjct: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGD 240
           WAERFGKQFLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLP DKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEF+PLKA+TTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 SDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAH 480
           S GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR VI A+DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRK 528
           GLKFTQSKIDYHSRAIP M+CR+ANPPVEVKA+ GAVMDVLK+H+L+++ K
Sbjct: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPK 528

BLAST of Moc08g45780 vs. NCBI nr
Match: XP_022946725.1 (threonine synthase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 932.9 bits (2410), Expect = 1.2e-267
Identity = 461/534 (86.33%), Postives = 489/534 (91.57%), Query Frame = 0

Query: 1   MASS-FSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGH 60
           MASS FS  ++LSPK  SLF RP  RNS   FT+ C SATSD A+SP+L SS P+ L   
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSAASPVLDSSLPLGLQNP 60

Query: 61  QLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQ 120
           Q A  +  D +   RS+  DG RNFSAKYVPFGSGFDS EWYSLD+IVYRS+SGGLLDVQ
Sbjct: 61  QFASVDIQDDSHRSRSVAADGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLF 180
           H+MEALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGD 240
           WAERFGK+FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEF+PLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 SDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAH 480
           S GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR VI+ +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQL 531
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+H+ R++ K +L
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKMEL 534

BLAST of Moc08g45780 vs. NCBI nr
Match: XP_022999212.1 (threonine synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 930.6 bits (2404), Expect = 6.0e-267
Identity = 457/532 (85.90%), Postives = 487/532 (91.54%), Query Frame = 0

Query: 2   ASSFSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGHQL 61
           +S FS  ++LSPK  SLF RP  RNS   FT+ C SATSD  +SP+L SS P+ +   Q 
Sbjct: 3   SSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQF 62

Query: 62  AGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHD 121
           A  +  D +   RS+ GDG RNFSAKYVPFGSGFDS EWYSLD+IVYRS+SGGLLDVQH+
Sbjct: 63  ACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHN 122

Query: 122 MEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWA 181
           MEALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLFWA
Sbjct: 123 MEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWA 182

Query: 182 ERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTS 241
           ERFGK+FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGDTS
Sbjct: 183 ERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTS 242

Query: 242 AALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELP 301
           AALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAELP
Sbjct: 243 AALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAELP 302

Query: 302 IYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLV 361
           IYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELGLV
Sbjct: 303 IYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLV 362

Query: 362 DKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSD 421
           DKIPRLVCAQAANANPLYLYYKSGWKEF+PLKASTTFASAIQIGDPVSIDRAVFALKNS 
Sbjct: 363 DKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKNSG 422

Query: 422 GIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGL 481
           GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR VI+ +DRTVVVSTAHGL
Sbjct: 423 GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHGL 482

Query: 482 KFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQL 531
           KFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+H+ R++ K +L
Sbjct: 483 KFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKMEL 534

BLAST of Moc08g45780 vs. NCBI nr
Match: KAG6599529.1 (Threonine synthase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 927.5 bits (2396), Expect = 5.1e-266
Identity = 460/534 (86.14%), Postives = 489/534 (91.57%), Query Frame = 0

Query: 1   MASS-FSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGH 60
           MASS FS  ++LSPK  SLF RP  RNS   FT+VC SATSD A+SP+L SS P+R+   
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIVCPSATSDSAASPVLDSSLPLRVQNP 60

Query: 61  QLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQ 120
           Q A  +  D +   RS+  DG RNFSAKYVPFGSGFDS E YSLD+IVYRS+SGGLLDVQ
Sbjct: 61  QFASVDIQDDSHRSRSLAADGGRNFSAKYVPFGSGFDSSECYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLF 180
           H+MEALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGD 240
           WAERFGK+FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEF+ LKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSALKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 SDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAH 480
           S GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR VI+ +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQL 531
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+H+ R++ K +L
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKMEL 534

BLAST of Moc08g45780 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.1e-236
Identity = 405/500 (81.00%), Postives = 444/500 (88.80%), Query Frame = 0

Query: 28  FTVVCSSATSDPASSPLLHSSPPIRLNGHQLAGANTLDQARPRRSIVGDGDRNFSAKYVP 87
           FT + ++AT+D A S   H  P         A  N  ++AR   S       NFSA+YVP
Sbjct: 34  FTPIKATATND-AISQQKHRRP---------ADENIREEARRHCS-----SHNFSARYVP 93

Query: 88  FGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDMEALKNFDGAYWRNLFDSRVGKTTWPYGS 147
           F +G +S EWYSLD+IVYRSRSGGLLDVQHDM+ALK FDG YWR+LFDSRVGKTTWPYGS
Sbjct: 94  FNAGPNSDEWYSLDEIVYRSRSGGLLDVQHDMDALKKFDGQYWRSLFDSRVGKTTWPYGS 153

Query: 148 GVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMNDVWVKHCGISHTGSFKDL 207
           GVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGM D+WVKHCGISHTGSFKDL
Sbjct: 154 GVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMTDLWVKHCGISHTGSFKDL 213

Query: 208 GMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAALSAYCAAAGIPSIVFLPTDKISIAQLV 267
           GMTVLVSQVNRLRKM++PV+GVGCASTGDTSAALSAYCA+AGIPSIVFLP +KIS+AQLV
Sbjct: 214 GMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLV 273

Query: 268 QPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWE 327
           QPIANGAFVLS+DTDFDGCM+LIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWE
Sbjct: 274 QPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWE 333

Query: 328 VPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFT 387
           VP+WVI+PGGNLGNIYAFYKGF+MC+ELGLVD+IPRLVCAQAANANPLYL+YKSGWK+F 
Sbjct: 334 VPEWVIVPGGNLGNIYAFYKGFQMCKELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFK 393

Query: 388 PLKASTTFASAIQIGDPVSIDRAVFALKNSDGIVEEATEEELMDATALADSTGMFICPHT 447
           P+KA+TTFASAIQIGDPVSIDRAVFAL+  +GIVEEATEEELMDA A ADSTGMFICPHT
Sbjct: 394 PVKANTTFASAIQIGDPVSIDRAVFALQQCNGIVEEATEEELMDAMAQADSTGMFICPHT 453

Query: 448 GVALTALIKLRNRDVIRANDRTVVVSTAHGLKFTQSKIDYHSRAIPSMDCRFANPPVEVK 507
           GVALTAL KLRN  VI   DRTVVVSTAHGLKFTQSKIDYHS+ I  M+CRFANPPVEVK
Sbjct: 454 GVALTALFKLRNSGVIAPTDRTVVVSTAHGLKFTQSKIDYHSKEIKDMECRFANPPVEVK 513

Query: 508 ADFGAVMDVLKDHILRRSRK 528
           ADFG+VMDVLK ++L ++ K
Sbjct: 514 ADFGSVMDVLKSYLLSQNSK 518

BLAST of Moc08g45780 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.2e-235
Identity = 408/517 (78.92%), Postives = 447/517 (86.46%), Query Frame = 0

Query: 9   STLSPKHDSLFPRPAA---RNSFTVVCSSATSDPASSPLLHS-SPPIRLNGHQLAGANTL 68
           S+L+PK D +    +    R+   V+  +A  +   +P+  +  PP R         N  
Sbjct: 12  SSLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPPHRTED------NIR 71

Query: 69  DQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDMEALKN 128
           D+AR  RS   +    FSAKYVPF +   S E YSLD+IVYRSRSGGLLDV+HDMEALK 
Sbjct: 72  DEARRNRS---NAVNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVEHDMEALKR 131

Query: 129 FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQ 188
           FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQ
Sbjct: 132 FDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQ 191

Query: 189 FLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAALSAY 248
           FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPV+GVGCASTGDTSAALSAY
Sbjct: 192 FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAY 251

Query: 249 CAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANS 308
           CA+AGIPSIVFLP +KIS+AQLVQPIANGAFVLS+DTDFDGCMKLIRE+TAELPIYLANS
Sbjct: 252 CASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAELPIYLANS 311

Query: 309 LNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDKIPRL 368
           LNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGFKMCQELGLVD+IPR+
Sbjct: 312 LNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGLVDRIPRM 371

Query: 369 VCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGIVEEA 428
           VCAQAANANPLYL+YKSGWK+F P+ ASTTFASAIQIGDPVSIDRAV+ALK  +GIVEEA
Sbjct: 372 VCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKCNGIVEEA 431

Query: 429 TEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKFTQSK 488
           TEEELMDA A ADSTGMFICPHTGVALTAL KLRN+ VI   DRTVVVSTAHGLKFTQSK
Sbjct: 432 TEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHGLKFTQSK 491

Query: 489 IDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHI 522
           IDYHS AIP M CRF+NPPV+VKADFGAVMDVLK ++
Sbjct: 492 IDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Moc08g45780 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.1e-230
Identity = 406/522 (77.78%), Postives = 446/522 (85.44%), Query Frame = 0

Query: 3   SSFSL--YSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG 62
           +SFSL   +T  P H     +P +  SFTV C+SA+  PA  P     P       +   
Sbjct: 2   ASFSLPHSATYFPSHSETSLKPHSAASFTVRCTSAS--PAVPPQTPQKP------RRSPD 61

Query: 63  ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME 122
            N  D+AR R   +    +N SA+YVPF +   S E YSLD+IVYRS+SG LLDVQHD  
Sbjct: 62  ENIRDEARRRPHQL----QNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFA 121

Query: 123 ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER 182
           ALK +DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAER
Sbjct: 122 ALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAER 181

Query: 183 FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA 242
           FGKQ+L MND+WVKHCGISHTGSFKDLGM+VLVSQVNRLRKMN+PVIGVGCASTGDTSAA
Sbjct: 182 FGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAA 241

Query: 243 LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 302
           LSAYCA+AGIPSIVFLP DKIS+AQLVQPIANGAFVLS+DTDFDGCM LIREVTAELPIY
Sbjct: 242 LSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIY 301

Query: 303 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK 362
           LANSLNSLRLEGQKTAAIEILQQF+W+VPDWVI+PGGNLGNIYAFYKGF MC+ELGLVD+
Sbjct: 302 LANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDR 361

Query: 363 IPRLVCAQAANANPLYLYYKSGWKE-FTPLKASTTFASAIQIGDPVSIDRAVFALKNSDG 422
           IPRLVCAQAANANPLYL+YKSG+KE F PLKA+TTFASAIQIGDPVSIDRAV+ALK S+G
Sbjct: 362 IPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNG 421

Query: 423 IVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLK 482
           IVEEATEEELMDATALADSTGMFICPHTGVALTAL+KLR   VI ANDRTVVVSTAHGLK
Sbjct: 422 IVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLK 481

Query: 483 FTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHI 522
           FTQSKIDYHS+ I  M CR ANPPV+VKA FG+VMDVLK+++
Sbjct: 482 FTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

BLAST of Moc08g45780 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.2e-72
Identity = 156/423 (36.88%), Postives = 244/423 (57.68%), Query Frame = 0

Query: 98  YSLDDIVYRSRSGGLLDVQHDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 157
           Y +D+I+Y    GGLL++ +D E +K+        + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 158 PEIDSDDIVSAFEGNSNLFWAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVN 217
           P  D   IVS  EG + L+      K+ LG+ +++VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 218 RLRKMNRPVIGVGCASTGDTSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVL 277
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 278 SMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGG 337
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W+VPD VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 338 NLGNIYAFYKGFKMCQELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFAS 397
           N GNI A +KGFK  +  G++D++P++   QA  A P+   ++   K+  P K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 398 AIQIGDPVSIDRAVFALKNSDGIVEEATEEELMDA-TALADSTGMFICPHTGVALTALIK 457
           AI+IG+PV+  +A+ A+ +S G  E  T+EE+++A   LA   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 458 LRNRDVIRANDRTVVVSTAHGLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDV 517
           L    +I  ++R V ++T HGLK             P    R +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 518 LKD 520
           LK+
Sbjct: 432 LKE 404

BLAST of Moc08g45780 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.1e-64
Identity = 153/391 (39.13%), Postives = 230/391 (58.82%), Query Frame = 0

Query: 98  YSLDDIVYR-SRSGGLLDVQHDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 157
           YS D+++Y  S+  GLLDV +D  ++K           D    KT  P    VW K   +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVW-KYAKL 72

Query: 158 LPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQV 217
           LP     + V+  EG + L+  +R  ++ +G+  ++VKH G++ TGSFKD GMTV V++ 
Sbjct: 73  LPV--EREPVTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVTKA 132

Query: 218 NRLRKMNRPVIGVGCASTGDTSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFV 277
             L  MN     V CASTG+TSAAL+ Y A AGIP +V LP  K+++ ++ Q + +GA V
Sbjct: 133 LEL-GMNT----VACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 278 LSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPG 337
           LS+  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D++VPD +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 338 GNLGNIYAFYKGFKMCQELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFA 397
           GN GNI A YKGF+  + LG+ D +P++   QA  + P+    KSG    TP +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 398 SAIQIGDPVSIDRAVFALKNSDGIVEEATEEELMDATA-LADSTGMFICPHTGVALTALI 457
           +AI+IG+PV+  +A+ A++ S G  E  T+EE++ A   LA   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 458 KLRNRDVIRANDRTVVVSTAHGLKFTQSKID 487
           KL +  VI  ++  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of Moc08g45780 vs. ExPASy TrEMBL
Match: A0A6J1DK79 (Threonine synthase OS=Momordica charantia OX=3673 GN=LOC111021858 PE=3 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG 60
           MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG
Sbjct: 25  MASSFSLYSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG 84

Query: 61  ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME 120
           ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME
Sbjct: 85  ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME 144

Query: 121 ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER 180
           ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER
Sbjct: 145 ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER 204

Query: 181 FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA 240
           FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA
Sbjct: 205 FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA 264

Query: 241 LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 300
           LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY
Sbjct: 265 LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 324

Query: 301 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK 360
           LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK
Sbjct: 325 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK 384

Query: 361 IPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGI 420
           IPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGI
Sbjct: 385 IPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGI 444

Query: 421 VEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKF 480
           VEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKF
Sbjct: 445 VEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKF 504

Query: 481 TQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ 540
           TQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ
Sbjct: 505 TQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQLYANFSFSESQ 564

BLAST of Moc08g45780 vs. ExPASy TrEMBL
Match: A0A6J1G4S3 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111450711 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.8e-268
Identity = 461/534 (86.33%), Postives = 489/534 (91.57%), Query Frame = 0

Query: 1   MASS-FSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGH 60
           MASS FS  ++LSPK  SLF RP  RNS   FT+ C SATSD A+SP+L SS P+ L   
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSAASPVLDSSLPLGLQNP 60

Query: 61  QLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQ 120
           Q A  +  D +   RS+  DG RNFSAKYVPFGSGFDS EWYSLD+IVYRS+SGGLLDVQ
Sbjct: 61  QFASVDIQDDSHRSRSVAADGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLF 180
           H+MEALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGD 240
           WAERFGK+FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEF+PLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 SDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAH 480
           S GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR VI+ +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQL 531
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+H+ R++ K +L
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKMEL 534

BLAST of Moc08g45780 vs. ExPASy TrEMBL
Match: A0A6J1KA83 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111493658 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.9e-267
Identity = 457/532 (85.90%), Postives = 487/532 (91.54%), Query Frame = 0

Query: 2   ASSFSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGHQL 61
           +S FS  ++LSPK  SLF RP  RNS   FT+ C SATSD  +SP+L SS P+ +   Q 
Sbjct: 3   SSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQF 62

Query: 62  AGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHD 121
           A  +  D +   RS+ GDG RNFSAKYVPFGSGFDS EWYSLD+IVYRS+SGGLLDVQH+
Sbjct: 63  ACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHN 122

Query: 122 MEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWA 181
           MEALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLFWA
Sbjct: 123 MEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWA 182

Query: 182 ERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTS 241
           ERFGK+FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGDTS
Sbjct: 183 ERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTS 242

Query: 242 AALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELP 301
           AALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAELP
Sbjct: 243 AALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAELP 302

Query: 302 IYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLV 361
           IYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELGLV
Sbjct: 303 IYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLV 362

Query: 362 DKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSD 421
           DKIPRLVCAQAANANPLYLYYKSGWKEF+PLKASTTFASAIQIGDPVSIDRAVFALKNS 
Sbjct: 363 DKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKNSG 422

Query: 422 GIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGL 481
           GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR VI+ +DRTVVVSTAHGL
Sbjct: 423 GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHGL 482

Query: 482 KFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQL 531
           KFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+H+ R++ K +L
Sbjct: 483 KFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKMEL 534

BLAST of Moc08g45780 vs. ExPASy TrEMBL
Match: A0A0A0LHR0 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G000280 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.5e-265
Identity = 459/534 (85.96%), Postives = 489/534 (91.57%), Query Frame = 0

Query: 1   MASS-FSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGH 60
           MASS FS  ++LSPK DSLF RP  RNS   FT+VCSSATSD A S ++ SS P+ ++ H
Sbjct: 1   MASSLFSFNTSLSPKPDSLFRRPTRRNSSSPFTLVCSSATSDSAPSHVIDSSLPLGVHTH 60

Query: 61  QLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQ 120
           Q       D +   RS+  D DRNFSAKYVPFGSG+DS EWYSLDDIVYRSRSGGLLDVQ
Sbjct: 61  QFQ-----DDSHRHRSVAVDADRNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLF 180
           H+M+ALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGD 240
           WAERFGKQFLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEF+PLKA+TTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 SDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAH 480
           S+GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR +I  NDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGNDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRKSQL 531
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKA+FGAVMDVLK+H+  ++ K +L
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSGKNPKFEL 529

BLAST of Moc08g45780 vs. ExPASy TrEMBL
Match: A0A5A7V3M7 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00370 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.7e-263
Identity = 455/531 (85.69%), Postives = 485/531 (91.34%), Query Frame = 0

Query: 1   MASS-FSLYSTLSPKHDSLFPRPAARNS---FTVVCSSATSDPASSPLLHSSPPIRLNGH 60
           MASS F   ++LSPK DSL  RP  RNS   FT+VCSSATSD A SP++ SS P+ ++ H
Sbjct: 1   MASSLFPFNTSLSPKPDSLLRRPTRRNSSSPFTLVCSSATSDSAPSPVIDSSLPLGVHTH 60

Query: 61  QLAGANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQ 120
           Q       D +   RS+  D   NFSAKYVPFGSG+DS EWYSLDDIVYRSRSGGLLDVQ
Sbjct: 61  QFQ-----DDSHRHRSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HDMEALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLF 180
           H+M+ALK FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGN+NLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNTNLF 180

Query: 181 WAERFGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGD 240
           WAERFGKQFLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV+GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLP DKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWKEF+PLKA+TTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 SDGIVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAH 480
           S+GIVEEATEEELMDATA ADSTGMFICPHTGVALTALIKLRNR +I  +DRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHILRRSRK 528
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKA+FGAVMDVLK+H+ R+S K
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSRKSPK 526

BLAST of Moc08g45780 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 816.2 bits (2107), Expect = 1.5e-236
Identity = 408/517 (78.92%), Postives = 447/517 (86.46%), Query Frame = 0

Query: 9   STLSPKHDSLFPRPAA---RNSFTVVCSSATSDPASSPLLHS-SPPIRLNGHQLAGANTL 68
           S+L+PK D +    +    R+   V+  +A  +   +P+  +  PP R         N  
Sbjct: 12  SSLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPPHRTED------NIR 71

Query: 69  DQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDMEALKN 128
           D+AR  RS   +    FSAKYVPF +   S E YSLD+IVYRSRSGGLLDV+HDMEALK 
Sbjct: 72  DEARRNRS---NAVNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVEHDMEALKR 131

Query: 129 FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQ 188
           FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQ
Sbjct: 132 FDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQ 191

Query: 189 FLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAALSAY 248
           FLGMND+WVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPV+GVGCASTGDTSAALSAY
Sbjct: 192 FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAY 251

Query: 249 CAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIYLANS 308
           CA+AGIPSIVFLP +KIS+AQLVQPIANGAFVLS+DTDFDGCMKLIRE+TAELPIYLANS
Sbjct: 252 CASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAELPIYLANS 311

Query: 309 LNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDKIPRL 368
           LNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGFKMCQELGLVD+IPR+
Sbjct: 312 LNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGLVDRIPRM 371

Query: 369 VCAQAANANPLYLYYKSGWKEFTPLKASTTFASAIQIGDPVSIDRAVFALKNSDGIVEEA 428
           VCAQAANANPLYL+YKSGWK+F P+ ASTTFASAIQIGDPVSIDRAV+ALK  +GIVEEA
Sbjct: 372 VCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKCNGIVEEA 431

Query: 429 TEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLKFTQSK 488
           TEEELMDA A ADSTGMFICPHTGVALTAL KLRN+ VI   DRTVVVSTAHGLKFTQSK
Sbjct: 432 TEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHGLKFTQSK 491

Query: 489 IDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHI 522
           IDYHS AIP M CRF+NPPV+VKADFGAVMDVLK ++
Sbjct: 492 IDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Moc08g45780 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 799.7 bits (2064), Expect = 1.5e-231
Identity = 406/522 (77.78%), Postives = 446/522 (85.44%), Query Frame = 0

Query: 3   SSFSL--YSTLSPKHDSLFPRPAARNSFTVVCSSATSDPASSPLLHSSPPIRLNGHQLAG 62
           +SFSL   +T  P H     +P +  SFTV C+SA+  PA  P     P       +   
Sbjct: 2   ASFSLPHSATYFPSHSETSLKPHSAASFTVRCTSAS--PAVPPQTPQKP------RRSPD 61

Query: 63  ANTLDQARPRRSIVGDGDRNFSAKYVPFGSGFDSPEWYSLDDIVYRSRSGGLLDVQHDME 122
            N  D+AR R   +    +N SA+YVPF +   S E YSLD+IVYRS+SG LLDVQHD  
Sbjct: 62  ENIRDEARRRPHQL----QNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFA 121

Query: 123 ALKNFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAER 182
           ALK +DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFWAER
Sbjct: 122 ALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAER 181

Query: 183 FGKQFLGMNDVWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVIGVGCASTGDTSAA 242
           FGKQ+L MND+WVKHCGISHTGSFKDLGM+VLVSQVNRLRKMN+PVIGVGCASTGDTSAA
Sbjct: 182 FGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAA 241

Query: 243 LSAYCAAAGIPSIVFLPTDKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 302
           LSAYCA+AGIPSIVFLP DKIS+AQLVQPIANGAFVLS+DTDFDGCM LIREVTAELPIY
Sbjct: 242 LSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIY 301

Query: 303 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFKMCQELGLVDK 362
           LANSLNSLRLEGQKTAAIEILQQF+W+VPDWVI+PGGNLGNIYAFYKGF MC+ELGLVD+
Sbjct: 302 LANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDR 361

Query: 363 IPRLVCAQAANANPLYLYYKSGWKE-FTPLKASTTFASAIQIGDPVSIDRAVFALKNSDG 422
           IPRLVCAQAANANPLYL+YKSG+KE F PLKA+TTFASAIQIGDPVSIDRAV+ALK S+G
Sbjct: 362 IPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNG 421

Query: 423 IVEEATEEELMDATALADSTGMFICPHTGVALTALIKLRNRDVIRANDRTVVVSTAHGLK 482
           IVEEATEEELMDATALADSTGMFICPHTGVALTAL+KLR   VI ANDRTVVVSTAHGLK
Sbjct: 422 IVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLK 481

Query: 483 FTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHI 522
           FTQSKIDYHS+ I  M CR ANPPV+VKA FG+VMDVLK+++
Sbjct: 482 FTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154650.10.0e+00100.00threonine synthase, chloroplastic-like [Momordica charantia][more]
XP_038891002.14.1e-26887.76threonine synthase, chloroplastic-like [Benincasa hispida][more]
XP_022946725.11.2e-26786.33threonine synthase, chloroplastic-like [Cucurbita moschata][more]
XP_022999212.16.0e-26785.90threonine synthase, chloroplastic-like [Cucurbita maxima][more]
KAG6599529.15.1e-26686.14Threonine synthase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
Q9MT281.1e-23681.00Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9S7B52.2e-23578.92Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ... [more]
Q9SSP52.1e-23077.78Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ... [more]
Q588605.2e-7236.88Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266... [more]
Q8TQD43.1e-6439.13Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /... [more]
Match NameE-valueIdentityDescription
A0A6J1DK790.0e+00100.00Threonine synthase OS=Momordica charantia OX=3673 GN=LOC111021858 PE=3 SV=1[more]
A0A6J1G4S35.8e-26886.33Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111450711 PE=3 SV=1[more]
A0A6J1KA832.9e-26785.90Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111493658 PE=3 SV=1[more]
A0A0A0LHR03.5e-26585.96Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G000280 PE=3 SV=1[more]
A0A5A7V3M75.7e-26385.69Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00... [more]
Match NameE-valueIdentityDescription
AT4G29840.11.5e-23678.92Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT1G72810.11.5e-23177.78Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 168..474
e-value: 6.3E-48
score: 163.7
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 156..482
e-value: 2.0E-106
score: 357.8
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 204..305
e-value: 2.0E-106
score: 357.8
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 97..520
IPR004450Threonine synthase-likeTIGRFAMTIGR00260TIGR00260coord: 155..479
e-value: 1.1E-60
score: 203.6
NoneNo IPR availablePIRSRPIRSR038945-2PIRSR038945-2coord: 148..462
e-value: 2.0E-70
score: 235.3
NoneNo IPR availablePIRSRPIRSR038945-1PIRSR038945-1coord: 148..462
e-value: 2.0E-70
score: 235.3
NoneNo IPR availablePANTHERPTHR10314:SF176THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 33..524
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 33..524
NoneNo IPR availableCDDcd01563Thr-synth_1coord: 150..480
e-value: 1.20112E-145
score: 420.076
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 195..209

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g45780.1Moc08g45780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004795 threonine synthase activity