Moc08g43880 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g43880
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionNEDD8 ultimate buster 1
Locationchr8: 33695072 .. 33700253 (+)
RNA-Seq ExpressionMoc08g43880
SyntenyMoc08g43880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAACTGAAGATTTCAGGGCCATGGGCTGGTGTGTTGGAGGTGGAGCTGGACAATTGGACTGTGCCAATGTTGAGGGAAGAAGTAGCAAGGCGATCAAACTGTGGAATTGAGTCGATTAATTTGATATGCGGTGGTAGAGTTTTGAAAGATGGCGATGGAAGCGAGAAGCTGATCAATTTGGGTGTTAAGAACAACTCTAGGATTCTTGCCAGGCGGGTCTCTACTGAGGAGGGCAAGTCACTTAAGGATGAGTTGATGGCTGAGGAAGAACGCTCTACCAGGCTCGCTCGAGTCAAGTAATTCTTTCACTTTTTTACTTCAATAAAGACGTTTATTGGATGCCTGTAGCTTAATTTGACTGAAAATTGAGTTTATTCAGATACCAATGCATTATGTGTTCATTTGATTTTGGGATGATTAGTTTTTAGAATTTTCATGGTCGGTCCCTGTGCTTGTTACTTCTATGAAGAAATTGGTCAAATTTTGTCGAAGCTTTTTGGGTCAAATGGGTTTGCTTTTCACAGTCATCTCTTAACTATTTTTTTGTAAAATATAACAATGGAAGTTGGATTTTGGATTACTGCTCGAAGTAAAAGATCATATTGGGTACGTATGAATAAAACCAGACTCCTAAACTTCCCTATGAAGTTGAAGACTGGTGTGGTTGTGTAACAATATGCTTTGTTGTACGATTTTGGCTCTCTTTATAACTTCAATTTTTTTTTTTTAAGACCTGCTTTGATCTTGGAAGCTTTTAGTTCAGCAATGAAAAGTAATTTTCATTTAGAGTTGGGATATGGTAGAATGCTAATCAAATGACAATCAACTTTTGGAGTATATTCTTCCCCATGGGAGGGCCATGAGTAATTGTGAAGATTTTAAGGACATTTTCTTCTGTGGTAGCTGGTTGTCAGTCTCATATTGTACTCATGTCAAGTGTAGATAGGCATGTAGGCTGTATGATAATTTCTGATTTCAATGTCTAATACTTTGTTTGGATTCTATCTTATCTGCTAGCATCTTCTCTAAACTTTCAGGGCAGCTGCCACTGCTCTAGCCAAGAGGCATGCTAATGGTTTCCTCCCTGTTGAAGATTTCAATATAGAACTTGAGGATCAAAGTGGACAGAAAGTTCATATGGGGTCTGAAACTGACCAAAGGTAGGGATAAAATCATGTTTCCTTCTTGTCAAATAAAATGTAAGAAATTAATTTGTTGCTGAGGATTTAAAGTCTTCTTTGGTCCTAAAATTTTGAGGCAAAATTGAATTCACATTCTACAGGGCAATCATGATGGGGTTGATGCTTCATGCAAACGCAAAACAGCTTATAAAAAAGGGAAATTACAAAGAAGCATTAGAAGTACTCGTCATGGGAGAGGTCTGTTGCCGGTTGATCACTTTAAATGATGTTAGGCATTAGCTATGAAAAATTTTAGTTGTTTCGCCCTTTTTGAAGATCCAGAAATAATTTAAGACGAACACAAAGATTAAAGCTTTTATCAATATCGACACCTCCCTCGAACGAAAAGGAAAAGATCAAATATAGCACCACAGTATAATTGATCTGCTGCATACATTTCTTTATTTCTGTTATGGTTGACAACTAATTTTGAAGTGCTCTGCCTTAATGGTCTTTATTTATTGACCAATGGTTCCAAATTCTAATTCCCATGACTGATTTTTTAAGGTCATTTTCTAACTTTGGATCAGTTCGATCATTTAGCTTGCAATGATTTTCAATATCAATTCTCCTTTTGTTTTCTTTCTTTACCTCTAAATTGTAGATGCTAAAACATGGTTTATGGTTTTATTATCCTAAGTTGTTATGCAAGATAATTGGTATCAGTTAAATTTTATTTGTTATGAGAGAATACTAATCAATGTGAATTCCTTTCTTGTAGGAGGCTTTCTCTCTTTGCAATCCAAAAGTCATTGAGGTAAATGACTCAATCTTTAACCTTGTTAGGTTTTTCTTCCCTGATTGATTGATTCTGCTCCCCCCTCCCCCCTAACTATTCGTTTATAACATTTCAACAAAATTTCGAATCAGACCTTTTTAAATTTATTATTCGTTAATTTGAGGCTGTTTTTGGTGCTGTCTACAGTTGTGATTCGATTTGAGGCATTGATGCTATGTGAGAATATACAAATTAATGATTTAGGGTAGTTGTTAGCCATAGCCTTTTCTTCTCCATGTCACCGAAGGTGGTGTTTCCATCAGGGTTTCTTAAATGCAGTTCTTAATGTTTTTCAACTCCTTGAGAATGTTTGATTTTCTTTCTGACAATTATGACTTTTCCTTTCTACCAGTTTGTTGACAACGTCCCGATACTCCAAATAGACATGGTGTGGTGCTATTTCATGCTAAAAGACATCAAGTGGCTCTCGGAGGCAGGACTACGCCTTGCGAAGGCTAGAGAAGGAATCGAACGAGCTCATGGGAAGGATTCTTCCCGTGTTCGACTCCTTCAAGCTGGTTGCCATCCCGAGCTTGCCTTGTAAGTGACTGCATTTATTGCCAATAGTTGGAGGGCGTGGTTTCCAGATTAAAGCTAATCACATTCTGTTATCATGTCAGACATTTGAGACTGGAGCTGCTGGAAGGGGTGGCTGCATACCATAGCTGTCAGTTTGATAAGGCTCAAAAGGCAATCAACTCGGCACAAGCAAAATATTTTCAGGTTAGTTTATCACATTACGGAATTAGTTGGATCGAGCTGAGGTATAATATATGTGAGGTAATTTGGTTTTATTGAATGCCTGCACTATAGTTGTAGTAATACCTATAGTTTCATTTTGATATTTACAGATGCCCTCTTGTGTTACTATGCAATTGGTGTTGTGTTTGGCATGTCTTTTAAAGTCCACGTGGAGGATTAAAAGTGTTTCTTGTAAAAATTGGTTGTGTTTTGGTTTAATTTAAAAAGTGCTTTAAATTTTTAACCCATTAAAATACTTTCTAAAATTTTCGATAATCAGAAGTAGTTTGAAAAATTCTAAACACATATAAAAAATACTCGAGCGTTTTTAGACAAAGCATTTATTTAGTGCTTACAAAATAAATGTTTCACTCAACAACACTTTTTCCAAAAGCATCCCAAACTAACACTGACCCTAAGAACAAACTGTCTTCAAATTTCAGCTACAAGTGCCAGACGAAGCATTATCACTTGTTATGAGTATGGGCTTTGAGGAAAGAGAAGCAAAGAGCGCACTGCGTATGTGCCATCAAGATGTAGCAAATGCTGTTGATTTTCTTGTTGGGGAAAGGGCAAAGAGAGAGAAAAAACGGGAAGAAGATATTCAACGCGAGAAGGAAATTGAGCAAGTGTTCTTAGCAAAACTGACTTCTATTTTCGTCTGGTAGGTTACATTCGATCTATAATATTAATAACCAGTATAAAATTTTCAGGGAGCAGAAACGCTATGGAATGACACCCTCCAAGAAGGCTGTTGATCTTCAGAGATTGAAAGAATTGGTATCTATAATAGGGTAAGTAGACTAACCAATATTGTTCAAAATGACAGAAGAAACGATCTTTGCATGTGATAACAAAGGCCTGGATTAGTAAGAGTCGTGTTGTGACTACTTCTCATGAAGACTTTCCATGAAATGGTCTACATAGTCTTTTAGGTTCTAGCTTCACTCGTTTTCGCCCGTTTCACAATGTTCTTCTCTATGTATTTTGCAAGGAAAAGTTAAAGGTAGGCTGGGTAGTCTTATGAGTTTAGAGCATCATGGATTGGTTTTGAAGAATGCCATCAGATTCTTATTCGATATGTCCCTTTCTACAGAAGGGAAATGTCTTTGGAAATGTCATTAATATTATTTTTCATGATCTTGAAATATCCATGGATACATGCTCAGCCAGTAAGATAGACAAGACTCGAGCCTAATATTCAAGGTTTGATTACATCTTTAGTAGATTCCTCCAATACTTGTCTTTCTATACCTACAATGAATCTTGTCTATCTTCCCGGATAGTCTGTTTGGAGCTGGAGGCACAGCAAATTCTGACTAAACGTGGTCATCTTGTAAGTGGGTGGTCAAGGTAATTTTGAGCCAATGCATTTTAAAGCAGGCAAATCTTGGTTTTAGAAAGGGACTCGGCACATACATTTGATATTTCGGTTTTTTCGGGTTGAAGCCTGACCTGGTAGACTTCTTAATGCTTAATGTTATTGATACGTTTTCCAGCTCACTTGGGAGGTTTAATTTAATGGCTTTGCAGCTCATCATCAGACAGATGTTTTCAATATATATTTTGTACCGTTGAGCTTCTTAAATTAAATTGTTTGGCAAACCATGGTATTCATTATGATAGAGCTTTCATCAATTGAGGTGAAATTGAGATGTTCAATATGAGCATTCTTGTAGTCATCATTCAAGGTGAAACTAACTTGGTTTTGCAGATATGAGAGGGAGTTAGCTGCTGAGGCCCTGAGAAGAAACGAGAATGACTTTCAGAAAGCATTGGACGATTTGACAAATCCAGAAACTAACTCTCTTATACAGGTGTCTTGAGTTTTGGATTGTATCCATAGTGTGAGTTGTTCTTTTTTTATTGAACTTCCATAGTGCTAAATCCCTGTCATCTGTGATTCTGTAGAACACTATTGAGTCGAGGAAAAGGAAACGACAACGTCAAACTGCAGATTCTACAGTTGATACACTTGTTCGCATGGGTTTCGATAGAACAAAAGGTCTCTATCTCTGAACAAGATGTTATTTTTAACAGGTTTCAAGACAGGATGTGTTTGATTGAACTAGTGATGCTGCTCTAACTTTAAATTTGTTATTTGTGTGCAGCCGTTGCAGCCTCTCAGGCTGCTGGTTCCTTAGAGCAAGCACTTCATCTATTATCACTTCCTGAGACAAATACTACGGTCGATGGGCAACCCGACTCTGCCTTTGCAGCCGTGGCTTTTGAGGGTGCAAATTCCTCCCTCTCAAACATTGATGGAAAAGAAGACATGTTAGACAACTTTGATGACGGGGAAGGCCCTTCCACTGCTGAAGAAGAACGAGACTCGGAGATGGAAAGCAAACTTGCCATGGAACTCAATGGTGCTACTGCCGTATCAGATTATGACATGGACGTAACAAAAGAAGGCGAAGCCATAACCGAGTACTTGGTTTTCTTGGATTCAGTGGAGAATCTTGAGAAAGCTTAA

mRNA sequence

ATGGCAAAACTGAAGATTTCAGGGCCATGGGCTGGTGTGTTGGAGGTGGAGCTGGACAATTGGACTGTGCCAATGTTGAGGGAAGAAGTAGCAAGGCGATCAAACTGTGGAATTGAGTCGATTAATTTGATATGCGGTGGTAGAGTTTTGAAAGATGGCGATGGAAGCGAGAAGCTGATCAATTTGGGTGTTAAGAACAACTCTAGGATTCTTGCCAGGCGGGTCTCTACTGAGGAGGGCAAGTCACTTAAGGATGAGTTGATGGCTGAGGAAGAACGCTCTACCAGGCTCGCTCGAGTCAAGGCAGCTGCCACTGCTCTAGCCAAGAGGCATGCTAATGGTTTCCTCCCTGTTGAAGATTTCAATATAGAACTTGAGGATCAAAGTGGACAGAAAGTTCATATGGGGTCTGAAACTGACCAAAGGGCAATCATGATGGGGTTGATGCTTCATGCAAACGCAAAACAGCTTATAAAAAAGGGAAATTACAAAGAAGCATTAGAAGTACTCGTCATGGGAGAGGAGGCTTTCTCTCTTTGCAATCCAAAAGTCATTGAGTTTGTTGACAACGTCCCGATACTCCAAATAGACATGGTGTGGTGCTATTTCATGCTAAAAGACATCAAGTGGCTCTCGGAGGCAGGACTACGCCTTGCGAAGGCTAGAGAAGGAATCGAACGAGCTCATGGGAAGGATTCTTCCCGTGTTCGACTCCTTCAAGCTGGTTGCCATCCCGAGCTTGCCTTACATTTGAGACTGGAGCTGCTGGAAGGGGTGGCTGCATACCATAGCTGTCAGTTTGATAAGGCTCAAAAGGCAATCAACTCGGCACAAGCAAAATATTTTCAGCTACAAGTGCCAGACGAAGCATTATCACTTGTTATGAGTATGGGCTTTGAGGAAAGAGAAGCAAAGAGCGCACTGCGTATGTGCCATCAAGATGTAGCAAATGCTGTTGATTTTCTTGTTGGGGAAAGGGCAAAGAGAGAGAAAAAACGGGAAGAAGATATTCAACGCGAGAAGGAAATTGAGCAACAGAAACGCTATGGAATGACACCCTCCAAGAAGGCTGTTGATCTTCAGAGATTGAAAGAATTGGTATCTATAATAGGATATGAGAGGGAGTTAGCTGCTGAGGCCCTGAGAAGAAACGAGAATGACTTTCAGAAAGCATTGGACGATTTGACAAATCCAGAAACTAACTCTCTTATACAGAACACTATTGAGTCGAGGAAAAGGAAACGACAACGTCAAACTGCAGATTCTACAGTTGATACACTTGTTCGCATGGGTTTCGATAGAACAAAAGCCGTTGCAGCCTCTCAGGCTGCTGGTTCCTTAGAGCAAGCACTTCATCTATTATCACTTCCTGAGACAAATACTACGGTCGATGGGCAACCCGACTCTGCCTTTGCAGCCGTGGCTTTTGAGGGTGCAAATTCCTCCCTCTCAAACATTGATGGAAAAGAAGACATGTTAGACAACTTTGATGACGGGGAAGGCCCTTCCACTGCTGAAGAAGAACGAGACTCGGAGATGGAAAGCAAACTTGCCATGGAACTCAATGGTGCTACTGCCGTATCAGATTATGACATGGACGTAACAAAAGAAGGCGAAGCCATAACCGAGTACTTGGTTTTCTTGGATTCAGTGGAGAATCTTGAGAAAGCTTAA

Coding sequence (CDS)

ATGGCAAAACTGAAGATTTCAGGGCCATGGGCTGGTGTGTTGGAGGTGGAGCTGGACAATTGGACTGTGCCAATGTTGAGGGAAGAAGTAGCAAGGCGATCAAACTGTGGAATTGAGTCGATTAATTTGATATGCGGTGGTAGAGTTTTGAAAGATGGCGATGGAAGCGAGAAGCTGATCAATTTGGGTGTTAAGAACAACTCTAGGATTCTTGCCAGGCGGGTCTCTACTGAGGAGGGCAAGTCACTTAAGGATGAGTTGATGGCTGAGGAAGAACGCTCTACCAGGCTCGCTCGAGTCAAGGCAGCTGCCACTGCTCTAGCCAAGAGGCATGCTAATGGTTTCCTCCCTGTTGAAGATTTCAATATAGAACTTGAGGATCAAAGTGGACAGAAAGTTCATATGGGGTCTGAAACTGACCAAAGGGCAATCATGATGGGGTTGATGCTTCATGCAAACGCAAAACAGCTTATAAAAAAGGGAAATTACAAAGAAGCATTAGAAGTACTCGTCATGGGAGAGGAGGCTTTCTCTCTTTGCAATCCAAAAGTCATTGAGTTTGTTGACAACGTCCCGATACTCCAAATAGACATGGTGTGGTGCTATTTCATGCTAAAAGACATCAAGTGGCTCTCGGAGGCAGGACTACGCCTTGCGAAGGCTAGAGAAGGAATCGAACGAGCTCATGGGAAGGATTCTTCCCGTGTTCGACTCCTTCAAGCTGGTTGCCATCCCGAGCTTGCCTTACATTTGAGACTGGAGCTGCTGGAAGGGGTGGCTGCATACCATAGCTGTCAGTTTGATAAGGCTCAAAAGGCAATCAACTCGGCACAAGCAAAATATTTTCAGCTACAAGTGCCAGACGAAGCATTATCACTTGTTATGAGTATGGGCTTTGAGGAAAGAGAAGCAAAGAGCGCACTGCGTATGTGCCATCAAGATGTAGCAAATGCTGTTGATTTTCTTGTTGGGGAAAGGGCAAAGAGAGAGAAAAAACGGGAAGAAGATATTCAACGCGAGAAGGAAATTGAGCAACAGAAACGCTATGGAATGACACCCTCCAAGAAGGCTGTTGATCTTCAGAGATTGAAAGAATTGGTATCTATAATAGGATATGAGAGGGAGTTAGCTGCTGAGGCCCTGAGAAGAAACGAGAATGACTTTCAGAAAGCATTGGACGATTTGACAAATCCAGAAACTAACTCTCTTATACAGAACACTATTGAGTCGAGGAAAAGGAAACGACAACGTCAAACTGCAGATTCTACAGTTGATACACTTGTTCGCATGGGTTTCGATAGAACAAAAGCCGTTGCAGCCTCTCAGGCTGCTGGTTCCTTAGAGCAAGCACTTCATCTATTATCACTTCCTGAGACAAATACTACGGTCGATGGGCAACCCGACTCTGCCTTTGCAGCCGTGGCTTTTGAGGGTGCAAATTCCTCCCTCTCAAACATTGATGGAAAAGAAGACATGTTAGACAACTTTGATGACGGGGAAGGCCCTTCCACTGCTGAAGAAGAACGAGACTCGGAGATGGAAAGCAAACTTGCCATGGAACTCAATGGTGCTACTGCCGTATCAGATTATGACATGGACGTAACAAAAGAAGGCGAAGCCATAACCGAGTACTTGGTTTTCTTGGATTCAGTGGAGAATCTTGAGAAAGCTTAA

Protein sequence

MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLINLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVEDFNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLCNPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQAGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFEEREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDLQRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTADSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGANSSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEAITEYLVFLDSVENLEKA
Homology
BLAST of Moc08g43880 vs. NCBI nr
Match: XP_022143095.1 (NEDD8 ultimate buster 1 [Momordica charantia])

HSP 1 Score: 1059.7 bits (2739), Expect = 8.8e-306
Identity = 556/557 (99.82%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI
Sbjct: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED
Sbjct: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIE+QKRYGMTPSKKAVDL
Sbjct: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA
Sbjct: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN
Sbjct: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA
Sbjct: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYLVFLDSVENLEKA
Sbjct: 541 ITEYLVFLDSVENLEKA 557

BLAST of Moc08g43880 vs. NCBI nr
Match: XP_023537732.1 (NEDD8 ultimate buster 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.0 bits (2327), Expect = 5.2e-258
Identity = 471/557 (84.56%), Postives = 509/557 (91.38%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW+GVL+VELDNWTV MLREE+ARRSNCG+ESINLICGGR+LKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LGVKNNSRILARRVSTEEGKSL  ELMAEEERS RLARVKAAATALAKRHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV++GSETDQRAIMMGLMLHANAKQLI+KGNYKEALEVL+MGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPKVIEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC FDKA++AINSAQ KY QLQVPDEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E EAK ALRMCHQDV  AVDFL  ER KREKKREEDI+R+KEI++QKRYGMTPSKKAVDL
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKEL S IGYE+ LAAEALRRNEND QKALDDL NPETNS+IQN +ESRKRKRQRQT 
Sbjct: 361 QRLKELAS-IGYEKNLAAEALRRNENDIQKALDDLINPETNSVIQNAVESRKRKRQRQTT 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D+ VD LV MGFDRT+A+AA QA GSLEQA++LLSLP+T +TVDGQPDS       E A+
Sbjct: 421 DAAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSGSEDAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SS+SNI+  +DMLDNFD+GEGPST EE RDSEMES+LA EL+GA+AVSDYDMDVTKEGEA
Sbjct: 481 SSISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N EKA
Sbjct: 541 ITEYLALLDSLMNSEKA 556

BLAST of Moc08g43880 vs. NCBI nr
Match: XP_022938166.1 (NEDD8 ultimate buster 1 [Cucurbita moschata])

HSP 1 Score: 899.4 bits (2323), Expect = 1.5e-257
Identity = 470/557 (84.38%), Postives = 510/557 (91.56%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW+GVL+VELDNWTV MLREE+ARRSNCG+ESINLICGGR+LKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LGVKNNSRILARRVSTEEGKSL  ELMAEEERS RLARVKAAATALAKRHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV++GSETDQRAIMMGLMLHANAKQLI+KGNYKEALEVL+MGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC FDKA++AINSAQ KY QLQVPDEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E EAK ALRMCHQDV  AVDFL  ER KR+KKREEDI+R+KEI++QKRYGMTPSKKAVDL
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKEL S IGYE+ LAAEALRRNEND QKALDDL NPETNS+IQN +ESRKRKRQRQT 
Sbjct: 361 QRLKELAS-IGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTT 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D+ VD LV MGFDRT+A+AA QA GSLEQA++LLSLP+T +TVDGQPDS     A E A+
Sbjct: 421 DAAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSASEYAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SS+SNI+  +DMLDNFD+GEGPST EE RDSEMES+LA EL+GA+AVSDYDMDVTKEGEA
Sbjct: 481 SSISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N EKA
Sbjct: 541 ITEYLALLDSLMNSEKA 556

BLAST of Moc08g43880 vs. NCBI nr
Match: KAG6586366.1 (NEDD8 ultimate buster 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 899.4 bits (2323), Expect = 1.5e-257
Identity = 470/557 (84.38%), Postives = 510/557 (91.56%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW+GVL+VELDNWTV MLREE+ARRSNCG+ESINLICGGR+LKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LGVKNNSRILARRVSTEEGKSL  ELMAEEERS RLARVKAAATALAKRHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV++GSETDQRAIMMGLMLHANAKQLI+KGNYKEALEVL+MGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC FDKA++AINSAQ KY QLQVPDEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E EAK ALRMCHQDV  AVDFL  ER KR+KKREEDI+R+KEI++QKRYGMTPSKKAVDL
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKEL S IGYE+ LAAEALRRNEND QKALDDL NPETNS+IQN +ESRKRKRQRQT 
Sbjct: 361 QRLKELAS-IGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTT 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D+ VD LV MGFDRT+A+AA QA GSLEQA++LLSLP+T +TVDGQPDS   A A E  +
Sbjct: 421 DAAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTASASEYGS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SS+SNI+  +DMLDNFD+GEGPST EE RDSEMES+LA EL+GA+AVSDYDMDVTKEGEA
Sbjct: 481 SSISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N EKA
Sbjct: 541 ITEYLALLDSLMNSEKA 556

BLAST of Moc08g43880 vs. NCBI nr
Match: XP_022965661.1 (NEDD8 ultimate buster 1 [Cucurbita maxima])

HSP 1 Score: 896.0 bits (2314), Expect = 1.7e-256
Identity = 470/557 (84.38%), Postives = 507/557 (91.02%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW+GVL+VELDNWTV MLREE+ARRSNCG+ESINLICGGR+LKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LGVKNNSRILA RVSTEEGKSL  ELMAEEERS RLARVKAAATALAKRHANGFLPVED
Sbjct: 61  DLGVKNNSRILAMRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV++GSETDQRAIMMGLMLHANAKQLI+KGNYKEALEVL+MGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPKVIEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC FDKA++AINSAQ KY QLQVPDEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E EAK ALRMCHQDV  AVDFL  ER KREKKREEDI+R+KEI++QKRYGMTPSKKAVDL
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKEL S IGYE+ LAAEALRRNEND QKALDDL NPETNS IQN +ESRKRKRQRQT 
Sbjct: 361 QRLKELAS-IGYEKNLAAEALRRNENDIQKALDDLINPETNSFIQNAVESRKRKRQRQTT 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D+ VD LV MGFDRT+A+AA QA GSLEQA++LLSLP+T +TVDGQPDS   A A E A+
Sbjct: 421 DAAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTASASEDAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SS+SNI+  +DMLDNFD+GEGPST EE RDSEMES+LA EL+ A+AVSDYDMDVTKEGEA
Sbjct: 481 SSISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDRASAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N  KA
Sbjct: 541 ITEYLALLDSLMNSAKA 556

BLAST of Moc08g43880 vs. ExPASy Swiss-Prot
Match: P54729 (NEDD8 ultimate buster 1 OS=Mus musculus OX=10090 GN=Nub1 PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 3.6e-28
Identity = 138/547 (25.23%), Postives = 252/547 (46.07%), Query Frame = 0

Query: 26  LREEVARRSNCGIESINLICGGRVLKDGDGSEKLINLGVKNNSRILARRVSTEEGKSLKD 85
           LR ++A         I ++   + L+ G   E+    GV +N + +   +   E    K+
Sbjct: 113 LRCQIAETFGFQENYIKIVINKKQLQLGKSLEE---QGVTHNVKAMVLELKQSEEDVRKN 172

Query: 86  ELMAEEE------RSTRLARVKAAATALAKRHANGFLPVEDFNIELEDQSGQKVHMGSET 145
             + EEE      +  R+ R K     LA+R      P     +++ +Q+G+ + +    
Sbjct: 173 LQLEEEEQNEAELKERRIQRTKRGLEILAERAEMVVDPETMPYLDIANQTGRSLRI-PPA 232

Query: 146 DQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLCNPKVIEFVDNVPILQIDMV 205
           +++A+M+ +  H   +  +K+  Y  AL  L+  +  F  C  ++++ VDN  +LQ+D+V
Sbjct: 233 ERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADRYFCECK-ELLDTVDNYAVLQLDIV 292

Query: 206 WCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQAGCHPELALHLRLELLEGV 265
           WCYF L+ ++ L +A  +L  A++  +  +G++  R+  ++  C  E  L LRL LL+G+
Sbjct: 293 WCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGI 352

Query: 266 AAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFEEREAKSALRMCHQDVANAV 325
             YHS   ++A++ +N A+  + +L +    +  ++ +GF  +EA+  LR C  +V +A 
Sbjct: 353 QNYHSGNGEEAREYLNKARQLFKELYIDPSKVHNLLQLGFTAQEARLGLRACDGNVDHAA 412

Query: 326 DFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDLQRLKELVSI--IGYERELA 385
             +         +REE  Q  KE ++++R            +RL+ + ++  +GY  + A
Sbjct: 413 THI-------SNRREELAQIRKEEKEKRR------------RRLENVNTLRGMGYSTQAA 472

Query: 386 AEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTADSTVDTLVRMGFDRTKA 445
            +AL +   +   AL  L +      +Q+  +     RQ   +  +++ LV MGFD   A
Sbjct: 473 KQALHQARGNLDDALKVLLSNPHMWWLQDA-DPENNSRQASPSQESINQLVYMGFDTVVA 532

Query: 446 VAASQAAGSLEQ------ALHLLSLPETNTTVDGQPDSAFAAVAFEGANSSLSNIDGKED 505
            AA +  G   Q      A H  SLP         PD     + F G +SS +       
Sbjct: 533 EAALRVFGGNVQLAAQTLAHHGGSLP---------PD-----LQFSGEDSSPTPSTSP-- 592

Query: 506 MLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGE--AITEYLVFLD 557
                 D  G S+A  + D E E+   +  +      DY +D T E E   I EYL +++
Sbjct: 593 -----SDSAGTSSASTDEDMETEAVNEILEDIPEHEEDY-LDSTLEDEEVIIAEYLSYVE 612

BLAST of Moc08g43880 vs. ExPASy Swiss-Prot
Match: Q9Y5A7 (NEDD8 ultimate buster 1 OS=Homo sapiens OX=9606 GN=NUB1 PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-27
Identity = 138/547 (25.23%), Postives = 250/547 (45.70%), Query Frame = 0

Query: 26  LREEVARRSNCGIESINLICGGRVLKDGDGSEKLINLGVKNNSRILARRVSTEEGKSLKD 85
           LR ++A         I ++   + L+ G   + L   GV +N + +   +   E  + K+
Sbjct: 112 LRSKIAETFGLQENYIKIVINKKQLQLG---KTLEEQGVAHNVKAMVLELKQSEEDARKN 171

Query: 86  ELMAEEE------RSTRLARVKAAATALAKRHANGFL-PVEDFNIELEDQSGQKVHMGSE 145
             + EEE      +  ++ R K     LAKR A   + P     +++ +Q+G+ + +   
Sbjct: 172 FQLEEEEQNEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRI-PP 231

Query: 146 TDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLCNPKVIEFVDNVPILQIDM 205
           ++++A+M+ +  H   +  +K+  Y  AL  L+  ++ F  C  ++++ VDN  +LQ+D+
Sbjct: 232 SERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDI 291

Query: 206 VWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQAGCHPELALHLRLELLEG 265
           VWCYF L+ ++ L +A  +L  A++  +  +G++  R+  ++  C  E  L LRL LL+G
Sbjct: 292 VWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQG 351

Query: 266 VAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFEEREAKSALRMCHQDVANA 325
           +  YHS    +A + +N A+  + +L +    +  ++ +GF  +EA+  LR C  +V +A
Sbjct: 352 IRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHA 411

Query: 326 VDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDLQRLKELVSIIGYERELAA 385
              +         +REE  Q  KE +++KR      ++  +++ LK +    GY    A 
Sbjct: 412 ATHIT-------NRREELAQIRKEEKEKKR------RRLENIRFLKGM----GYSTHAAQ 471

Query: 386 EALRRNENDFQKALDD-LTNPETNSLIQNTIESRKRKRQRQTADSTVDTLVRMGFDRTKA 445
           + L     +  +AL   L+NP+   L  N        RQ   +   +D LV MGFD   A
Sbjct: 472 QVLHAASGNLDEALKILLSNPQMWWL--NDSNPETDNRQESPSQENIDRLVYMGFDALVA 531

Query: 446 VAASQA-AGSLEQALHLL-----SLPETNTTVDGQPDSAFAAVAFEGANSSLSNIDGKED 505
            AA +   G+++ A   L     SLP                         LS  D    
Sbjct: 532 EAALRVFRGNVQLAAQTLAHNGGSLP---------------------PELPLSPEDSLSP 591

Query: 506 MLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGE--AITEYLVFLD 557
              +  D  G S+A  + D E E+   +  +      DY +D T E E   I EYL +++
Sbjct: 592 PATSPSDSAGTSSASTDEDMETEAVNEILEDIPEHEEDY-LDSTLEDEEIIIAEYLSYVE 613

BLAST of Moc08g43880 vs. ExPASy TrEMBL
Match: A0A6J1CMT5 (NEDD8 ultimate buster 1 OS=Momordica charantia OX=3673 GN=LOC111013048 PE=4 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.3e-306
Identity = 556/557 (99.82%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI
Sbjct: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED
Sbjct: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIE+QKRYGMTPSKKAVDL
Sbjct: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA
Sbjct: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN
Sbjct: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA
Sbjct: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYLVFLDSVENLEKA
Sbjct: 541 ITEYLVFLDSVENLEKA 557

BLAST of Moc08g43880 vs. ExPASy TrEMBL
Match: A0A6J1FCD2 (NEDD8 ultimate buster 1 OS=Cucurbita moschata OX=3662 GN=LOC111444333 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.4e-258
Identity = 470/557 (84.38%), Postives = 510/557 (91.56%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW+GVL+VELDNWTV MLREE+ARRSNCG+ESINLICGGR+LKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LGVKNNSRILARRVSTEEGKSL  ELMAEEERS RLARVKAAATALAKRHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV++GSETDQRAIMMGLMLHANAKQLI+KGNYKEALEVL+MGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC FDKA++AINSAQ KY QLQVPDEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E EAK ALRMCHQDV  AVDFL  ER KR+KKREEDI+R+KEI++QKRYGMTPSKKAVDL
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKEL S IGYE+ LAAEALRRNEND QKALDDL NPETNS+IQN +ESRKRKRQRQT 
Sbjct: 361 QRLKELAS-IGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTT 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D+ VD LV MGFDRT+A+AA QA GSLEQA++LLSLP+T +TVDGQPDS     A E A+
Sbjct: 421 DAAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSASEYAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SS+SNI+  +DMLDNFD+GEGPST EE RDSEMES+LA EL+GA+AVSDYDMDVTKEGEA
Sbjct: 481 SSISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N EKA
Sbjct: 541 ITEYLALLDSLMNSEKA 556

BLAST of Moc08g43880 vs. ExPASy TrEMBL
Match: A0A6J1HMA3 (NEDD8 ultimate buster 1 OS=Cucurbita maxima OX=3661 GN=LOC111465491 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.2e-257
Identity = 470/557 (84.38%), Postives = 507/557 (91.02%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW+GVL+VELDNWTV MLREE+ARRSNCG+ESINLICGGR+LKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LGVKNNSRILA RVSTEEGKSL  ELMAEEERS RLARVKAAATALAKRHANGFLPVED
Sbjct: 61  DLGVKNNSRILAMRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV++GSETDQRAIMMGLMLHANAKQLI+KGNYKEALEVL+MGEEAFSLC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPKVIEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVRLLQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC FDKA++AINSAQ KY QLQVPDEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E EAK ALRMCHQDV  AVDFL  ER KREKKREEDI+R+KEI++QKRYGMTPSKKAVDL
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           QRLKEL S IGYE+ LAAEALRRNEND QKALDDL NPETNS IQN +ESRKRKRQRQT 
Sbjct: 361 QRLKELAS-IGYEKNLAAEALRRNENDIQKALDDLINPETNSFIQNAVESRKRKRQRQTT 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D+ VD LV MGFDRT+A+AA QA GSLEQA++LLSLP+T +TVDGQPDS   A A E A+
Sbjct: 421 DAAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTASASEDAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SS+SNI+  +DMLDNFD+GEGPST EE RDSEMES+LA EL+ A+AVSDYDMDVTKEGEA
Sbjct: 481 SSISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDRASAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N  KA
Sbjct: 541 ITEYLALLDSLMNSAKA 556

BLAST of Moc08g43880 vs. ExPASy TrEMBL
Match: A0A1S3C5V6 (NEDD8 ultimate buster 1 OS=Cucumis melo OX=3656 GN=LOC103497022 PE=4 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 9.4e-245
Identity = 451/557 (80.97%), Postives = 499/557 (89.59%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW GVL+VELDNWTV MLREE+ARRSNC +ESINLI GGR+LKDGDGSEKL+
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVRMLREEIARRSNCSVESINLISGGRILKDGDGSEKLV 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LG+KNNSRILARRVSTEEGK+LK+ELMAEEERSTRLARVKAAATALA+RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FNIELEDQSGQKV+ GSETDQ+AIMMGLMLHANAKQLI+KGNYK+ALEVLVMGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRMLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC+FDKA++AINSA+AKYFQLQV DEALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E+EAK ALRMCHQDV  AVDFL  ER KRE+KREEDI+R++EI +QK YGMTPSKKAV+L
Sbjct: 301 EKEAKRALRMCHQDVCKAVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           Q L++L S IGYE+ELAAEALRRNEND QKALDDL +PE+NS+IQN IESRKRKRQR+  
Sbjct: 361 QILEKLTS-IGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAIESRKRKRQRKRV 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D  VD LVRMGFD+T+A AA +A GSLEQAL +LS P TNT V GQPDS  A +A EGA+
Sbjct: 421 DGAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGTNTAVHGQPDSVNAVLASEGAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SSLSNI   ++MLD  DD  G S  EE+RDS ME +LA ELNGATAVSDYDMDVTKEGEA
Sbjct: 481 SSLSNIAENQEMLDTLDDEGGSSIGEEDRDSVMEDELARELNGATAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N E A
Sbjct: 541 ITEYLGLLDSMMNSENA 556

BLAST of Moc08g43880 vs. ExPASy TrEMBL
Match: A0A0A0LI64 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008690 PE=4 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.1e-242
Identity = 443/557 (79.53%), Postives = 502/557 (90.13%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60
           MAKLKISGPW GVL+VELDNWTV MLREE+ARRSNCG+ESINLI GGR+LKDGDGSEKL+
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60

Query: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120
           +LG+KNNSRILARRV+ EEGK+LK+ELMAEEERS+RLARVKAAATALA+RHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180
           FN+ELEDQSGQKV+ GSETDQ+AIMMGLMLHANAKQLI+KGNYK+ALEVLVMGEEAF+LC
Sbjct: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+++AINSA+AKYFQLQV D+ALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300

Query: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAVDL 360
           E+EAK ALRMC+QDV  AVDFL  ER +RE+KREED++R++EI +QK YGMTPSKKAV+L
Sbjct: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420
           Q L++L S IGYE+ELAAEALRRNEND QKALDDL +PE+NS+IQN +ESRKRKRQR+ A
Sbjct: 361 QILEKLAS-IGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRA 420

Query: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480
           D  VD LV MGFD+T+A AA +A GSLEQA+ +LS P TN+TVDGQPDS  AA+  EGA+
Sbjct: 421 DDAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGAS 480

Query: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540
           SSLSNI   ++MLD  DD  GPS  EE+RD EME +LA ELNGATAVSDYDMDVTKEGEA
Sbjct: 481 SSLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEA 540

Query: 541 ITEYLVFLDSVENLEKA 558
           ITEYL  LDS+ N E A
Sbjct: 541 ITEYLGLLDSMMNSENA 556

BLAST of Moc08g43880 vs. TAIR 10
Match: AT2G12550.1 (ubiquitin-associated (UBA)/TS-N domain-containing protein )

HSP 1 Score: 571.2 bits (1471), Expect = 8.9e-163
Identity = 321/562 (57.12%), Postives = 418/562 (74.38%), Query Frame = 0

Query: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLK-DGDGSEKL 60
           MAKLK++G W G+LEVEL+NWT+ MLR+EVA+RS    ESI LI  G++LK DGD +  L
Sbjct: 1   MAKLKVAGTWGGILEVELENWTLNMLRDEVAKRSGLDPESIKLIFAGKILKDDGDATLTL 60

Query: 61  INLGVKNNSRILA-RRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPV 120
             LG+K NS+IL+ R+ + EEGKS    +MAEEER+ RL+RVKAAATAL+KRHA+G LP+
Sbjct: 61  SQLGIKENSKILSCRQAAPEEGKS----IMAEEERARRLSRVKAAATALSKRHADGSLPI 120

Query: 121 EDFNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFS 180
           +D+NIELEDQ G KV  G+ETDQ A MMGLMLH  AK LI+   Y +A+EVL M EE+F 
Sbjct: 121 QDYNIELEDQGGHKVQFGTETDQSAAMMGLMLHTKAKSLIEIEMYSDAVEVLAMAEESFL 180

Query: 181 LCNPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRL 240
           LC+PK++E VDN+P+++ID+VWCYF+L+DIK LS+AG+RL +AR+G+ERAHGKD SRVRL
Sbjct: 181 LCDPKILELVDNLPMMEIDIVWCYFLLRDIKCLSDAGVRLVRARKGLERAHGKDLSRVRL 240

Query: 241 LQAGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMG 300
           LQAG  PE+A++LRLELLEGV AYH+ Q DKA  A+ SA AK+ QLQ+PDE LSLVM MG
Sbjct: 241 LQAGQSPEMAIYLRLELLEGVVAYHTGQNDKALNALKSAHAKFLQLQIPDETLSLVMGMG 300

Query: 301 FEEREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEQQKRYGMTPSKKAV 360
           F+E++AK ALR+ +QD+A++VDFL+ ERAK+ +KRE+D+QR+ EI +QK+YG+TP KKAV
Sbjct: 301 FQEKDAKRALRLNNQDIASSVDFLIEERAKKAQKREQDLQRQSEILEQKKYGVTPMKKAV 360

Query: 361 DLQRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQ 420
           D+Q L+ LVS IGY RELAAE+LRRNEND QKALD LT+P+ NS IQ  IESRKRKRQ Q
Sbjct: 361 DMQMLERLVS-IGYARELAAESLRRNENDIQKALDILTDPKVNSTIQAYIESRKRKRQEQ 420

Query: 421 TADSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLL-----SLPETNTTVDGQPDSAFAA 480
               +V  LV MGF+R KA +A +A GS E  +  L     + P T TT      S+   
Sbjct: 421 LVGISVAELVSMGFERGKATSALEAGGSREDTIQRLLSASVANPGTTTTSVINATSSTNN 480

Query: 481 VAFEGA---NSSLSNIDGKEDMLDNFDDGEGPST--AEEERDSEMESKLAMELNGATAVS 540
           V  E +     +  + + K++  D+  +    S+   EEERDSE+E ++A E+    A+S
Sbjct: 481 VGAESSGFGGGAEQDSEMKDETTDDIANRASTSSDGMEEERDSEIEDEIADEIARVDALS 540

Query: 541 DYDMDVTKEGEAITEYLVFLDS 551
            YD+++ KE EAI EYL  LD+
Sbjct: 541 AYDINLDKEIEAINEYLAMLDA 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143095.18.8e-30699.82NEDD8 ultimate buster 1 [Momordica charantia][more]
XP_023537732.15.2e-25884.56NEDD8 ultimate buster 1 [Cucurbita pepo subsp. pepo][more]
XP_022938166.11.5e-25784.38NEDD8 ultimate buster 1 [Cucurbita moschata][more]
KAG6586366.11.5e-25784.38NEDD8 ultimate buster 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022965661.11.7e-25684.38NEDD8 ultimate buster 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P547293.6e-2825.23NEDD8 ultimate buster 1 OS=Mus musculus OX=10090 GN=Nub1 PE=1 SV=2[more]
Q9Y5A71.1e-2725.23NEDD8 ultimate buster 1 OS=Homo sapiens OX=9606 GN=NUB1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CMT54.3e-30699.82NEDD8 ultimate buster 1 OS=Momordica charantia OX=3673 GN=LOC111013048 PE=4 SV=1[more]
A0A6J1FCD27.4e-25884.38NEDD8 ultimate buster 1 OS=Cucurbita moschata OX=3662 GN=LOC111444333 PE=4 SV=1[more]
A0A6J1HMA38.2e-25784.38NEDD8 ultimate buster 1 OS=Cucurbita maxima OX=3661 GN=LOC111465491 PE=4 SV=1[more]
A0A1S3C5V69.4e-24580.97NEDD8 ultimate buster 1 OS=Cucumis melo OX=3656 GN=LOC103497022 PE=4 SV=1[more]
A0A0A0LI641.1e-24279.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008690 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G12550.18.9e-16357.12ubiquitin-associated (UBA)/TS-N domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 327..347
NoneNo IPR availableGENE3D3.10.20.90coord: 8..84
e-value: 4.3E-6
score: 28.3
NoneNo IPR availableGENE3D1.10.8.10coord: 287..325
e-value: 4.3E-10
score: 41.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..519
NoneNo IPR availableCDDcd14291UBA1_NUB1_likecoord: 288..322
e-value: 6.56502E-8
score: 46.673
NoneNo IPR availableCDDcd14270UBAcoord: 363..393
e-value: 0.00639848
score: 32.3229
IPR015940Ubiquitin-associated domainSMARTSM00165uba_6coord: 359..397
e-value: 0.22
score: 20.0
coord: 420..456
e-value: 5.6
score: 8.7
coord: 287..324
e-value: 9.3E-5
score: 31.8
IPR015940Ubiquitin-associated domainPFAMPF00627UBAcoord: 289..322
e-value: 1.7E-6
score: 27.7
IPR015940Ubiquitin-associated domainPROSITEPS50030UBAcoord: 285..325
score: 11.920369
IPR039749NEDD8 ultimate buster 1PANTHERPTHR12948NEDD8 ULTIMATE BUSTER-1 BS4 PROTEINcoord: 20..550
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 22..70
score: 8.654931
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 20..71
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 262..325
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 347..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g43880.1Moc08g43880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:2000058 regulation of ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0031593 polyubiquitin modification-dependent protein binding
molecular_function GO:0005515 protein binding