Moc08g43810 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43810
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	acidic mammalian chitinase-like
Location	chr8: 33659418 .. 33660411 (-)
RNA-Seq Expression	Moc08g43810
Synteny	Moc08g43810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCATCCAACAACGGTCGTGAAGGGTGGTTATTGGTTGGGCAGCACGGCGGAGAAACTTCCTGCTGCCTGTATTGATGCTACGTTGTACAGCCATCTATTCTGTGGATGTGTAGAAATCGATGCCACAAGTCATGAAGTGACGATTCCTTCCATGGAGACCAAGAAGTTTTCTGTAAATGTGAGATCAAGCAATCCGAACGTGAAAATTTTGCTGTCAATTAGAGGAGACAGTAAAGTATTCTCTAAAATGGCAAAGGAGCCTACACTACGCGATGCGTTCATCAAATCTTCGATCCACGCGACAGTTGCTGGTGGGTTTAACGGCCTGGATTTATAATGGCTGTACCCATCTTCTCCAATTGACATGGAAAACTTTGAAACCCTGATTAGGAAATGGCGAGCGGCGGCTATGGATGACAGCTAGAACCTACAACCGATCAAAGTTGATCCTGGTGGCTTTGGTGTCGAGCTTACCCTACATTGAGAAGGGTGTTGAGTACCCAGGTGAAGCAATCAAACAAAACTTGGACTGGGTGAACCTCATTTGTTACGACTTTTTCACCCCAGTTTTCCTTCTCTCGTTACACTGGACCATCCTCCGCATCGCAAGTTTCAGGATCGATTACTGGATTCGTCTAGTTCCTCCTAAGCAGATTGTGTTGGGAATCCCTTTCCACGGTTGGTCCTGGAAACTCGACGACGCTCTCCAGCACGACGTGTTTCCCAACGCCTATAGCCAACCTGCATCTGATGCGCCATGCCACGGCTACGACGACATCAAGAAGTTCATAGATGAGACTGAGGTTGAAGTTGTCCCAAATGACCCCAGATTTGTGATTGCTTACACCTTTGCCAATACGACCTGGATTACCTACGAAAGTGAATCCACCATTCCTGTGAAAATTGAATTTGCAAAGCAAAAGAATCTGCTTACACCTTTGCCAATACGACCCGGATTACCTACGAAAGTGAATCCACCATTCCTGTGA

mRNA sequence

ATGTCTCATCCAACAACGGTCGTGAAGGGTGGTTATTGGTTGGGCAGCACGGCGGAGAAACTTCCTGCTGCCTGTATTGATGCTACGTTGTACAGCCATCTATTCTGTGGATGTGTAGAAATCGATGCCACAAGTCATGAAGTGACGATTCCTTCCATGGAGACCAAGAAGTTTTCTGTAAATGTGAGATCAAGCAATCCGAACGTGAAAATTTTGCTGTCAATTAGAGGAGACAGTAAAGTATTCTCTAAAATGGCAAAGGAGCCTACACTACGCGATGCGTTCATCAAATCTTCGATCCACGCGACAGTTGCTGGAAATGGCGAGCGGCGGCTATGGATGACAGCTAGAACCTACAACCGATCAAAGTTGATCCTGGTGGCTTTGGTGTCGAGCTTACCCTACATTGAGAAGGGTGTTGAGTACCCAGGTGAAGCAATCAAACAAAACTTGGACTGGGTGAACCTCATTTGTTACGACTTTTTCACCCCAGTTTTCCTTCTCTCGTTACACTGGACCATCCTCCGCATCGCAAGTTTCAGGATCGATTACTGGATTCGTCTAGTTCCTCCTAAGCAGATTGTGTTGGGAATCCCTTTCCACGGTTGGTCCTGGAAACTCGACGACGCTCTCCAGCACGACGTGTTTCCCAACGCCTATAGCCAACCTGCATCTGATGCGCCATGCCACGGCTACGACGACATCAAGAAGTTCATAGATGAGACTGAGGTTGAAGTTGTCCCAAATGACCCCAGATTTGTGATTGCTTACACCTTTGCCAATACGACCTGGATTACCTACGAAAGTGAATCCACCATTCCTGTGAAAATTGAATTTGCAAAGCAAAAGAATCTGCTTACACCTTTGCCAATACGACCCGGATTACCTACGAAAGTGAATCCACCATTCCTGTGA

Coding sequence (CDS)

ATGTCTCATCCAACAACGGTCGTGAAGGGTGGTTATTGGTTGGGCAGCACGGCGGAGAAACTTCCTGCTGCCTGTATTGATGCTACGTTGTACAGCCATCTATTCTGTGGATGTGTAGAAATCGATGCCACAAGTCATGAAGTGACGATTCCTTCCATGGAGACCAAGAAGTTTTCTGTAAATGTGAGATCAAGCAATCCGAACGTGAAAATTTTGCTGTCAATTAGAGGAGACAGTAAAGTATTCTCTAAAATGGCAAAGGAGCCTACACTACGCGATGCGTTCATCAAATCTTCGATCCACGCGACAGTTGCTGGAAATGGCGAGCGGCGGCTATGGATGACAGCTAGAACCTACAACCGATCAAAGTTGATCCTGGTGGCTTTGGTGTCGAGCTTACCCTACATTGAGAAGGGTGTTGAGTACCCAGGTGAAGCAATCAAACAAAACTTGGACTGGGTGAACCTCATTTGTTACGACTTTTTCACCCCAGTTTTCCTTCTCTCGTTACACTGGACCATCCTCCGCATCGCAAGTTTCAGGATCGATTACTGGATTCGTCTAGTTCCTCCTAAGCAGATTGTGTTGGGAATCCCTTTCCACGGTTGGTCCTGGAAACTCGACGACGCTCTCCAGCACGACGTGTTTCCCAACGCCTATAGCCAACCTGCATCTGATGCGCCATGCCACGGCTACGACGACATCAAGAAGTTCATAGATGAGACTGAGGTTGAAGTTGTCCCAAATGACCCCAGATTTGTGATTGCTTACACCTTTGCCAATACGACCTGGATTACCTACGAAAGTGAATCCACCATTCCTGTGAAAATTGAATTTGCAAAGCAAAAGAATCTGCTTACACCTTTGCCAATACGACCCGGATTACCTACGAAAGTGAATCCACCATTCCTGTGA

Protein sequence

MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSVNVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATVAGNGERRLWMTARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWVNLICYDFFTPVFLLSLHWTILRIASFRIDYWIRLVPPKQIVLGIPFHGWSWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIAYTFANTTWITYESESTIPVKIEFAKQKNLLTPLPIRPGLPTKVNPPFL

Homology

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BLAST of Moc08g43810 vs. NCBI nr
Match: XP_022143253.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 319.3 bits (817), Expect = 3.6e-83
Identity = 180/325 (55.38%), Postives = 207/325 (63.69%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL STA KLPA+ IDATLY+HLFCG  EID T HEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATV--AGNGERRLWM---- 120
            V+ SNP+VK LLSIRGDSKVFS +AK PT RD F+ SSI A V    +G    W+    
Sbjct: 61  KVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 120

Query: 121 --------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                                AR  NR + ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 PTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPVFLLSLHWT-----------ILRIASFRIDYWIRLVPPKQIVLGIPFHGW 240
           NL+CYDF+TP    S  +T               A F I+ W + VPPK+IV GIPFHGW
Sbjct: 181 NLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGW 240

Query: 241 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFID--ETEVEVVPNDPRFVIAYTFAN 287
           +W L +AL+HDVF  A    AS      Y ++KKFID   +  + +PNDPRF IAY +A 
Sbjct: 241 AWTLANALEHDVFSKATGLAASGQ--MDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAE 300

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BLAST of Moc08g43810 vs. NCBI nr
Match: XP_022143252.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 315.8 bits (808), Expect = 3.9e-82
Identity = 179/333 (53.75%), Postives = 209/333 (62.76%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL +TA KLPA+ IDATLY+HLFCG  EI+ATSHEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHA------------------ 120
            V+ SNPNVK LLSI GDSKVFS +AK PT RD FIKSSI A                  
Sbjct: 61  KVKMSNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSS 120

Query: 121 --------TVAGNGERRLWMTARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                   T+ G   + +   ARTYNR+  ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 PTDMQNFETLIGKWRQAVVDDARTYNRAARILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPV------------FLLSLHWTILRIASFRIDYWIRLVPPKQIVLGIPFHG 240
           NL+CYDF+TP              L + +   L  A F I+ W + VP  +IV GIPFHG
Sbjct: 181 NLVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLS-ACFGIESWTKYVPATKIVFGIPFHG 240

Query: 241 WSWKLDDALQHDVFPNAYSQPASDAPC-------HGYDDIKKFI--DETEVEVVPNDPRF 287
           W+W+L D  ++DVF  A    ASD            Y ++KKFI  +  + + +PNDPRF
Sbjct: 241 WAWQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPRF 300

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BLAST of Moc08g43810 vs. NCBI nr
Match: XP_022143018.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 313.5 bits (802), Expect = 2.0e-81
Identity = 178/325 (54.77%), Postives = 204/325 (62.77%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL STA KLPA+ IDATLY+HLFCG  EID T HEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDXTXHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATV--AGNGERRLWM---- 120
            V+ SNP+VK LLSIRGDSKVFS +AK PT RD F+ SSI A V    +G    W+    
Sbjct: 61  KVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 120

Query: 121 --------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                                AR  NR + ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 XTDMQNFKXLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPVFLLSLHWT-----------ILRIASFRIDYWIRLVPPKQIVLGIPFHGW 240
           NL+CYDF+TP    S  +T               A F I+ W + VP K+IV GIPFHGW
Sbjct: 181 NLVCYDFYTPAEGSSPDYTGPSSALFNRXSNSLSAXFGIESWTKYVPXKKIVFGIPFHGW 240

Query: 241 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFID--ETEVEVVPNDPRFVIAYTFAN 287
           +W L +A +HDVF  A    AS      Y ++KKFID   +    +PNDPRF IAY +A 
Sbjct: 241 AWTLANAXEHDVFSKATGXAASGQ--MDYSEVKKFIDAHASVSXNIPNDPRFGIAYAYAE 300

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BLAST of Moc08g43810 vs. NCBI nr
Match: AAM18075.1 (class V chitinase [Momordica charantia])

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-78
Identity = 174/324 (53.70%), Postives = 201/324 (62.04%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL STA KLPA+ IDATLY+HLFCG  EIDATSHEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATV--AGNGERRLWM---- 120
            V+ SNP+VK LLSIRGDSKVFS +AK PT RD F+ SSI A V    +G    W+    
Sbjct: 61  KVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 120

Query: 121 --------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                                AR  NR + ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 PTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPVFLLSLHWT-----------ILRIASFRIDYWIRLVPPKQIVLGIPFHGW 240
           NL+CYDF+TP    S  +T               A F I+ W + VP K+IV GIPFHGW
Sbjct: 181 NLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGW 240

Query: 241 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEV-VPNDPRFVIAYTFANT 287
           +W L +A +HD+F  A     S      Y ++K      + E  +PNDPRF IAY +  T
Sbjct: 241 AWTLANAAEHDMFSKA---TGSCVWPMDYSEVKSHRRARQCEYNIPNDPRFGIAYAYVET 300

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BLAST of Moc08g43810 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-67
Identity = 154/331 (46.53%), Postives = 193/331 (58.31%), Query Frame = 0


Query: 2   SHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMET---KKF 61
           S PT+ V GGYWL ST ++LP + IDA L++HLFCG  EID  S+EVT+P+ +     +F
Sbjct: 5   SKPTSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQF 64

Query: 62  SVNVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATVAG--NGERRLWM-- 121
           S  VR SNP+VK LLSIRGD  VF+KMA EPT RD FI+SSI     G  NG    W+  
Sbjct: 65  SSTVRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYP 124

Query: 122 ----------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLD 181
                                  A+TY+R + ILVA VS+LPY+E  + YP E IKQNLD
Sbjct: 125 SSEEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLD 184

Query: 182 WVNLICYDFFTPVFLLS-------LHWTILR--IASFRIDYWIRL---VPPKQIVLGIPF 241
           WVNLI YDF+TP    S       LH  + R   A+F ID WI+    +P KQIV GIPF
Sbjct: 185 WVNLISYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPF 244

Query: 242 HGWSWKLDDALQHDVFPNAYSQPASDAPCH-----GYDDIKKFIDETEVEVVPNDPRFVI 287
           HGW+WKL +  +HDVF  A      D            ++K+FI +   + V +D R+ I
Sbjct: 245 HGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSI 304

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BLAST of Moc08g43810 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 101/330 (30.61%), Postives = 150/330 (45.45%), Query Frame = 0


Query: 6   TVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV---NV 65
           TVVK  YW    A + P   ID++L++HLFC   ++++ +++VT+ S    KFS     V
Sbjct: 26  TVVKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTV 85

Query: 66  RSSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIH------------------ 125
           +  NP+VK LLSI G   D   ++ MA  PT R +FI SSI                   
Sbjct: 86  QRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPS 145

Query: 126 --------ATVAGNGERRLWMTARTYNRSKLILVALV--SSLPYIEKGVEYPGEAIKQNL 185
                    T+       +   A +  + +L+L A V  S+  Y    V YP  A+  +L
Sbjct: 146 SATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSL 205

Query: 186 DWVNLICYDFFTPVFLLSLHWTILR--------------IASFRIDYWIRL-VPPKQIVL 245
           DWVNL+ YDF+ P       W+ +                       WI+  +P K+ VL
Sbjct: 206 DWVNLMAYDFYGP------GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVL 265

Query: 246 GIPFHGWSWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIA 287
           G P++G++W+L +A  H  +        S     GY  I+KFI +     V N    V  
Sbjct: 266 GFPYYGYAWRLTNANSHSYYAPTTGAAISPDGSIGYGQIRKFIVDNGATTVYNS-TVVGD 325

[Close]

BLAST of Moc08g43810 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 97.8 bits (242), Expect = 2.2e-19
Identity = 89/330 (26.97%), Postives = 144/330 (43.64%), Query Frame = 0


Query: 5   TTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCV-------EIDATSHEVTIPSMETKK 64
           TT VKG YW+ +     P + ID +L++H+F   V       +++    + T  +     
Sbjct: 34  TTRVKGIYWIENPL--FPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTT 93

Query: 65  FSVNVRSSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIHA--TVAGNGERRL 124
           F+   ++  P +  LLSI G   +S +F+ +A +PT R  FI S+I    T   +G    
Sbjct: 94  FTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFD 153

Query: 125 W-------------------------MTARTYNRSKLILVALVSSLPYIEKGVE--YPGE 184
           W                          TA T     L+  A+  ++ +   G    YP +
Sbjct: 154 WEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVD 213

Query: 185 AIKQNLDWVNLICYDF--------FTPVFLLSLHWTILRIASFRIDYWIR-LVPPKQIVL 244
           +I +NLDWVN++ YD           P  +      +  ++   +  WIR  V P++IV+
Sbjct: 214 SINKNLDWVNVMSYDLRGSGSNVTGAPSGMFDSKSNVSVVSG--LFSWIRGGVAPEKIVM 273

Query: 245 GIPFHGWSWKLDDALQHDV-FPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVI 286
           G+P +G SWKL D   H +  PN    P  D     Y  +  F  +   +VV  D     
Sbjct: 274 GMPLYGKSWKLQDPNVHGIGAPNVGPGPGVDGGM-AYFQVVDFNKQMGAKVV-YDKETGS 333

[Close]

BLAST of Moc08g43810 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 1.9e-18
Identity = 86/321 (26.79%), Postives = 142/321 (44.24%), Query Frame = 0


Query: 8   VKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETK---KFSVNVRS 67
           V+  YW          + ID   ++H++   V+    S  + I     K   K+   + +
Sbjct: 55  VRAAYW--PAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYD-GIHN 114

Query: 68  SNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIH-------------------- 127
             P V  LLSI G   ++ +FS+MA     R +FI S+IH                    
Sbjct: 115 IRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 128 ------ATVAGNGERRLWMTARTYNRSKLILVALV---SSLPYIEKGV-EYPGEAIKQNL 187
                 A +     + L + A T  +S+L+L + V   S++  I  G   YP  AI++ L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 188 DWVNLICYDF-----FTPVFLLSLHWTILRIAS-FRIDYWIRL-VPPKQIVLGIPFHGWS 247
           DW + +C+D+         F  +L+     I++ + I  WI   VP +++V+G+P +G +
Sbjct: 235 DWASPMCFDYNGAWANETGFNAALYDPNSNISTKYGIGSWIGSGVPAEKLVMGLPLYGRA 294

Query: 248 WKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIAYTFANTTW 286
           W+L D   H V   A            YD+I  F  +T  +VV  D   V  Y+++ TTW
Sbjct: 295 WELKDPNDHGVGAKAVGPAVDTDGSMDYDEILVFNKDTGAKVV-YDEVAVSFYSYSGTTW 354

[Close]

BLAST of Moc08g43810 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.0e-16
Identity = 81/322 (25.16%), Postives = 141/322 (43.79%), Query Frame = 0


Query: 8   VKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETK---KFSVNVRS 67
           V+  YW     +    + ID   ++H+    ++ +  S ++ I     K    F   +R 
Sbjct: 40  VRSAYW--PAGDDFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 68  SNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIHAT------------------ 127
            +P VK LLSI G   +S +FS++A     R+ FI S+I                     
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 128 --------VAGNGERRLWMTARTYNRSKLILVALV---SSLPYIEK--GVEYPGEAIKQN 187
                   +       L+  A+   + +L+L + V   S++  I K     YP +AI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 188 LDWVNLICYDFF-----TPVFLLSLHWTILRIA-SFRIDYWIRL-VPPKQIVLGIPFHGW 247
           LDW + +C+D+         F  +L+ +   I+ +F +  WI+  V P+++V+G+  +G 
Sbjct: 220 LDWASPMCFDYHGTWDNNTDFNAALYDSKSEISTNFGLHSWIKSGVRPEKLVMGLALYGR 279

Query: 248 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIAYTFANTT 286
           +W+L D   + V   A            Y++I KF  ++   VV  D   +  Y++A TT
Sbjct: 280 AWELKDPNVNGVGAEAVGPATDTDGSMNYNEILKFNKQSGANVV-YDKVAISFYSYAGTT 339

[Close]

BLAST of Moc08g43810 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.1e-14
Identity = 83/335 (24.78%), Postives = 141/335 (42.09%), Query Frame = 0


Query: 2   SHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPS---METKKF 61
           S   T VKG YWL +     P   +D +L++H+F   +  +  ++++ I S   +    F
Sbjct: 29  SSSITRVKGIYWLENPF--FPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTF 88

Query: 62  SVNVRSSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIHA--TVAGNGERRLW 121
           +   ++ +P    L SI G   +S + + +A +P    AFI S+I    T   +G    W
Sbjct: 89  TKTFKTKSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDW 148

Query: 122 MTAR------------------------TYNRSKLILVALV---SSLPYIEKGVEYPGEA 181
              +                        T  R  L+L A V    +     +   YP  +
Sbjct: 149 EFPKNTKEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNS 208

Query: 182 IKQNLDWVNLICYDF--------------FTPVFLLSLHWTILRIASFRIDYWIRL-VPP 241
           I +NLDWVN++ Y+               F P   +S+   +L         WI   V P
Sbjct: 209 INENLDWVNVMSYELRGPRSNKTGAPSGTFDPKSNVSVVSGLLS--------WIHSGVVP 268

Query: 242 KQIVLGIPFHGWSWKLDDALQHDV-FPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPND 286
           +++V+G+P +G SWKL D   H +  P+  S P  +     Y  +  F  +   + V  D
Sbjct: 269 EKLVMGMPLYGKSWKLRDPNVHGIGAPSVGSGPGVNG-LMAYFQVLDFNRQKSAK-VEYD 328

[Close]

BLAST of Moc08g43810 vs. ExPASy TrEMBL
Match: A0A6J1CQ93 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.7e-83
Identity = 180/325 (55.38%), Postives = 207/325 (63.69%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL STA KLPA+ IDATLY+HLFCG  EID T HEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATV--AGNGERRLWM---- 120
            V+ SNP+VK LLSIRGDSKVFS +AK PT RD F+ SSI A V    +G    W+    
Sbjct: 61  KVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 120

Query: 121 --------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                                AR  NR + ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 PTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPVFLLSLHWT-----------ILRIASFRIDYWIRLVPPKQIVLGIPFHGW 240
           NL+CYDF+TP    S  +T               A F I+ W + VPPK+IV GIPFHGW
Sbjct: 181 NLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGW 240

Query: 241 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFID--ETEVEVVPNDPRFVIAYTFAN 287
           +W L +AL+HDVF  A    AS      Y ++KKFID   +  + +PNDPRF IAY +A 
Sbjct: 241 AWTLANALEHDVFSKATGLAASGQ--MDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAE 300

[Close]

BLAST of Moc08g43810 vs. ExPASy TrEMBL
Match: A0A6J1CQ95 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.9e-82
Identity = 179/333 (53.75%), Postives = 209/333 (62.76%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL +TA KLPA+ IDATLY+HLFCG  EI+ATSHEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHA------------------ 120
            V+ SNPNVK LLSI GDSKVFS +AK PT RD FIKSSI A                  
Sbjct: 61  KVKMSNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSS 120

Query: 121 --------TVAGNGERRLWMTARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                   T+ G   + +   ARTYNR+  ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 PTDMQNFETLIGKWRQAVVDDARTYNRAARILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPV------------FLLSLHWTILRIASFRIDYWIRLVPPKQIVLGIPFHG 240
           NL+CYDF+TP              L + +   L  A F I+ W + VP  +IV GIPFHG
Sbjct: 181 NLVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLS-ACFGIESWTKYVPATKIVFGIPFHG 240

Query: 241 WSWKLDDALQHDVFPNAYSQPASDAPC-------HGYDDIKKFI--DETEVEVVPNDPRF 287
           W+W+L D  ++DVF  A    ASD            Y ++KKFI  +  + + +PNDPRF
Sbjct: 241 WAWQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPRF 300

[Close]

BLAST of Moc08g43810 vs. ExPASy TrEMBL
Match: A0A6J1CPP6 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013006 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 9.4e-82
Identity = 178/325 (54.77%), Postives = 204/325 (62.77%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL STA KLPA+ IDATLY+HLFCG  EID T HEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDXTXHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATV--AGNGERRLWM---- 120
            V+ SNP+VK LLSIRGDSKVFS +AK PT RD F+ SSI A V    +G    W+    
Sbjct: 61  KVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 120

Query: 121 --------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                                AR  NR + ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 XTDMQNFKXLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPVFLLSLHWT-----------ILRIASFRIDYWIRLVPPKQIVLGIPFHGW 240
           NL+CYDF+TP    S  +T               A F I+ W + VP K+IV GIPFHGW
Sbjct: 181 NLVCYDFYTPAEGSSPDYTGPSSALFNRXSNSLSAXFGIESWTKYVPXKKIVFGIPFHGW 240

Query: 241 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFID--ETEVEVVPNDPRFVIAYTFAN 287
           +W L +A +HDVF  A    AS      Y ++KKFID   +    +PNDPRF IAY +A 
Sbjct: 241 AWTLANAXEHDVFSKATGXAASGQ--MDYSEVKKFIDAHASVSXNIPNDPRFGIAYAYAE 300

[Close]

BLAST of Moc08g43810 vs. ExPASy TrEMBL
Match: Q8S2V9 (Class V chitinase OS=Momordica charantia OX=3673 GN=Chi5 PE=2 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 5.7e-79
Identity = 174/324 (53.70%), Postives = 201/324 (62.04%), Query Frame = 0


Query: 1   MSHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV 60
           MSHP T+V GGYWL STA KLPA+ IDATLY+HLFCG  EIDATSHEVTIPS E   FS 
Sbjct: 1   MSHP-TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSE 60

Query: 61  NVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATV--AGNGERRLWM---- 120
            V+ SNP+VK LLSIRGDSKVFS +AK PT RD F+ SSI A V    +G    W+    
Sbjct: 61  KVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 120

Query: 121 --------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLDWV 180
                                AR  NR + ILVA V +LPYIEK VEYPGEAIKQNLDWV
Sbjct: 121 PTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWV 180

Query: 181 NLICYDFFTPVFLLSLHWT-----------ILRIASFRIDYWIRLVPPKQIVLGIPFHGW 240
           NL+CYDF+TP    S  +T               A F I+ W + VP K+IV GIPFHGW
Sbjct: 181 NLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGW 240

Query: 241 SWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEV-VPNDPRFVIAYTFANT 287
           +W L +A +HD+F  A     S      Y ++K      + E  +PNDPRF IAY +  T
Sbjct: 241 AWTLANAAEHDMFSKA---TGSCVWPMDYSEVKSHRRARQCEYNIPNDPRFGIAYAYVET 300

[Close]

BLAST of Moc08g43810 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 7.8e-68
Identity = 154/331 (46.53%), Postives = 193/331 (58.31%), Query Frame = 0


Query: 2   SHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMET---KKF 61
           S PT+ V GGYWL ST ++LP + IDA L++HLFCG  EID  S+EVT+P+ +     +F
Sbjct: 5   SKPTSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQF 64

Query: 62  SVNVRSSNPNVKILLSIRGDSKVFSKMAKEPTLRDAFIKSSIHATVAG--NGERRLWM-- 121
           S  VR SNP+VK LLSIRGD  VF+KMA EPT RD FI+SSI     G  NG    W+  
Sbjct: 65  SSTVRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYP 124

Query: 122 ----------------------TARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLD 181
                                  A+TY+R + ILVA VS+LPY+E  + YP E IKQNLD
Sbjct: 125 SSEEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLD 184

Query: 182 WVNLICYDFFTPVFLLS-------LHWTILR--IASFRIDYWIRL---VPPKQIVLGIPF 241
           WVNLI YDF+TP    S       LH  + R   A+F ID WI+    +P KQIV GIPF
Sbjct: 185 WVNLISYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPF 244

Query: 242 HGWSWKLDDALQHDVFPNAYSQPASDAPCH-----GYDDIKKFIDETEVEVVPNDPRFVI 287
           HGW+WKL +  +HDVF  A      D            ++K+FI +   + V +D R+ I
Sbjct: 245 HGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSI 304

[Close]

BLAST of Moc08g43810 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 146.0 bits (367), Expect = 5.0e-35
Identity = 108/327 (33.03%), Postives = 156/327 (47.71%), Query Frame = 0


Query: 2   SHPTTVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV- 61
           S   TVVK  YW   +  + P A ID++L++HLFC   +I+  +++V + S    KFS  
Sbjct: 20  SSAQTVVKATYWFAES--ESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTF 79

Query: 62  --NVRSSNPNVKILLSIRGD---SKVFSKMAKEPTLRDAFIKSSIH-------------- 121
              VR  NP VK LLSI GD   +  F+ MA  PT R  FI SSI               
Sbjct: 80  TQTVRRRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNW 139

Query: 122 -----ATVAGNGER--RLW-----MTARTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQ 181
                 T   N  +  R W       AR+  + +L+L A V    Y    V +P  A+  
Sbjct: 140 KYPSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAV-FYSYSYYSVLHPVNAVAD 199

Query: 182 NLDWVNLICYDFF---------TPVFLLSLHWTILRIASFRIDYWIRL-VPPKQIVLGIP 241
           +LDWVNL+ YDF+         +P  L     T        +  W +  +P K+ VLG P
Sbjct: 200 SLDWVNLVAYDFYESGSSRVTCSPAPLYD-PITTGPSGDAGVRAWTQAGLPAKKAVLGFP 259

Query: 242 FHGWSWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIAYTF 287
            +G++W L DA  H+ + N+     S     GYD I++FI + +  +V N    V  Y +
Sbjct: 260 LYGYAWCLTDAKNHNYYANSSGPAISPDGSIGYDQIRRFIVDNKATMVYNS-NLVQNYCY 319

[Close]

BLAST of Moc08g43810 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 138.7 bits (348), Expect = 8.0e-33
Identity = 101/330 (30.61%), Postives = 150/330 (45.45%), Query Frame = 0


Query: 6   TVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMETKKFSV---NV 65
           TVVK  YW    A + P   ID++L++HLFC   ++++ +++VT+ S    KFS     V
Sbjct: 26  TVVKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTV 85

Query: 66  RSSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIH------------------ 125
           +  NP+VK LLSI G   D   ++ MA  PT R +FI SSI                   
Sbjct: 86  QRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPS 145

Query: 126 --------ATVAGNGERRLWMTARTYNRSKLILVALV--SSLPYIEKGVEYPGEAIKQNL 185
                    T+       +   A +  + +L+L A V  S+  Y    V YP  A+  +L
Sbjct: 146 SATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSL 205

Query: 186 DWVNLICYDFFTPVFLLSLHWTILR--------------IASFRIDYWIRL-VPPKQIVL 245
           DWVNL+ YDF+ P       W+ +                       WI+  +P K+ VL
Sbjct: 206 DWVNLMAYDFYGP------GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVL 265

Query: 246 GIPFHGWSWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIA 287
           G P++G++W+L +A  H  +        S     GY  I+KFI +     V N    V  
Sbjct: 266 GFPYYGYAWRLTNANSHSYYAPTTGAAISPDGSIGYGQIRKFIVDNGATTVYNS-TVVGD 325

[Close]

BLAST of Moc08g43810 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 137.5 bits (345), Expect = 1.8e-32
Identity = 107/321 (33.33%), Postives = 156/321 (48.60%), Query Frame = 0


Query: 7   VVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIPSMET---KKFSVNVR 66
           VVK  YW  +T    PA  ID++L++HLFC   +++A S+E+TI +        F+  V+
Sbjct: 6   VVKASYWFPAT--DFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQ 65

Query: 67  SSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIHATVAGN------------- 126
             NP+VK LLSI G   D   F+ MA  P  R +FI+S+I  TVA +             
Sbjct: 66  QRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTI--TVARSYGFHGLDLDWEYP 125

Query: 127 -GERRL---------WMTA--RTYNRSKLILVALVSSLPYIE--KGVEYPGEAIKQNLDW 186
             E  +         W +A     N S    + L +++ Y    +GV YP  AI  +LDW
Sbjct: 126 RNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSNYQGVPYPVLAISNSLDW 185

Query: 187 VNLICYDFFTPVFL------LSLHW-TILRIASFRIDYWIRL-VPPKQIVLGIPFHGWSW 246
           +NL+ YDF+ P +        SL+  T  R     +  W    +P K+ VLG P++GW+W
Sbjct: 186 INLMAYDFYGPGWSTVTGPPASLYLPTDGRSGDSGVRDWTEAGLPAKKAVLGFPYYGWAW 245

Query: 247 KLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIAYTFANTTWI 287
            L D   +    N      SD     Y  ++ +I +     V +D   V  Y +A TTWI
Sbjct: 246 TLADPDVNGYDANTTGPAISDDGEISYRQLQTWIVDNGATKV-HDDMMVGDYCYAGTTWI 305

[Close]

BLAST of Moc08g43810 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 129.8 bits (325), Expect = 3.7e-30
Identity = 98/324 (30.25%), Postives = 143/324 (44.14%), Query Frame = 0


Query: 6   TVVKGGYWLGSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIP---SMETKKFSVNV 65
           ++VK  YW+       PA  ID+T ++HLFC   ++D+++HEVTI    S +   F+  V
Sbjct: 12  SIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTV 71

Query: 66  RSSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSI------------------- 125
           +  N +V+ LLSI G   D  V + MA     R AFI SSI                   
Sbjct: 72  KDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPS 131

Query: 126 -HATVAGNGER-RLWMTA------RTYNRSKLILVALVSSLPYIEKGVEYPGEAIKQNLD 185
               +A  G+  + W  A      RT     L+  A+  S  Y   G EYP +AI  NLD
Sbjct: 132 NDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYY--GEEYPVQAIADNLD 191

Query: 186 WVNLICYDFF---------TPVFLLSLHWTILRIASFRIDYWIRL-VPPKQIVLGIPFHG 245
           +VN++ YDF+          P  L        R     +  W+   +P K+ VLG  + G
Sbjct: 192 FVNIMAYDFYGPGWSPVTGPPAALFDPSNPAGRSGDSGLSKWLEAKLPAKKAVLGFSYCG 251

Query: 246 WSWKLDDALQHDVFPNAYSQPASDAPCHGYDDIKKFIDETEVEVVPNDPRFVIAYTFANT 287
           W+W L+DA  +           S      Y  I+ +I +       +DP  +  Y +  T
Sbjct: 252 WAWTLEDAENNGYDAATDGAAISSDGSITYAKIRNYIIDNGAATF-HDPAVIGFYCYVGT 311

[Close]

BLAST of Moc08g43810 vs. TAIR 10
Match: AT4G19720.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 129.4 bits (324), Expect = 4.9e-30
Identity = 105/332 (31.63%), Postives = 149/332 (44.88%), Query Frame = 0


Query: 5   TTVVKGGYWL---------GSTAEKLPAACIDATLYSHLFCGCVEIDATSHEVTIP---S 64
           T VVK  YW          GS   +  A  ID+TL++HLFC   ++D  ++ V +     
Sbjct: 3   TEVVKASYWFPDGASSPTTGSVVPQSSAVLIDSTLFTHLFCAFADLDPQTNSVVVSGAHE 62

Query: 65  METKKFSVNVRSSNPNVKILLSIRG---DSKVFSKMAKEPTLRDAFIKSSIHAT--VAGN 124
            E   F+  V+  NP+V+ LLSI G   D   F+ MA  PT R +FI S+I +      +
Sbjct: 63  QEFSNFTKIVKKKNPHVQTLLSIGGRNADKSAFASMASNPTSRKSFIWSAISSARYYRFD 122

Query: 125 GERRLWM------------------------TARTYNRSKLILVALVSSLPYIEKGVEYP 184
           G   +W                          A    R  L+L A V   P +   V YP
Sbjct: 123 GLDLVWKYPKDDVEMRNFGQLLEQWREAIEDDAERTERMPLLLTAAVYYSP-VYDSVSYP 182

Query: 185 GEAIKQNLDWVNLICYDFFT-------PVFLLSLHWTILRIASFRIDYWIRL-VPPKQIV 244
              IK+ LDWVNLI YDF++       P  L            + +  WI+  +P K+ V
Sbjct: 183 IREIKKKLDWVNLIAYDFYSSSTTIGPPAALFDPSNPKGPCGDYGLKEWIKAGLPAKKAV 242

Query: 245 LGIPFHGWSWKLDDALQHDVFPNAYSQPASDAP-CHGYDDIKKFIDETEVEVVPNDPRFV 287
           LG P+ GW+W L     +D    A S+ A+ A     YD IK+ I + +   V  D   V
Sbjct: 243 LGFPYVGWTWSLGSG--NDA---ATSRVATSAEGSINYDQIKRLIVDHKARPV-FDSTVV 302

The following BLAST results are available for this feature:

BLAST of Moc08g43810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143253.1	3.6e-83	55.38	chitotriosidase-1-like [Momordica charantia]	[more]
XP_022143252.1	3.9e-82	53.75	acidic mammalian chitinase-like [Momordica charantia]	[more]
XP_022143018.1	2.0e-81	54.77	acidic mammalian chitinase-like [Momordica charantia]	[more]
AAM18075.1	1.2e-78	53.70	class V chitinase [Momordica charantia]	[more]
XP_022159350.1	1.6e-67	46.53	chitinase-3-like protein 2 [Momordica charantia]	[more]

BLAST of Moc08g43810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	1.1e-31	30.61	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
U5N4E3	2.2e-19	26.97	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A1B1J8Z2	1.9e-18	26.79	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072VEP0	1.0e-16	25.16	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]
A0A072UR65	8.1e-14	24.78	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]

BLAST of Moc08g43810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQ93	1.7e-83	55.38	chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1	[more]
A0A6J1CQ95	1.9e-82	53.75	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 P...	[more]
A0A6J1CPP6	9.4e-82	54.77	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013006 P...	[more]
Q8S2V9	5.7e-79	53.70	Class V chitinase OS=Momordica charantia OX=3673 GN=Chi5 PE=2 SV=1	[more]
A0A6J1DYG4	7.8e-68	46.53	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]

BLAST of Moc08g43810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19820.1	5.0e-35	33.03	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19810.1	8.0e-33	30.61	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	1.8e-32	33.33	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	3.7e-30	30.25	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19720.1	4.9e-30	31.63	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 7..286 e-value: 3.0E-12 score: 56.7
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 115..285 e-value: 2.7E-25 score: 91.4
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 5..105 e-value: 5.5E-14 score: 54.0
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 116..287
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 116..287 coord: 4..102
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 4..102
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 120..285 e-value: 6.8E-20 score: 72.0
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 11..100 e-value: 2.4E-8 score: 34.1
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 7..304 score: 10.154351
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 200..267 e-value: 2.7E-25 score: 91.4
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 9..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43810.1	Moc08g43810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding