Moc08g43780 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43780
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	acidic mammalian chitinase-like
Location	chr8: 33648288 .. 33649331 (+)
RNA-Seq Expression	Moc08g43780
Synteny	Moc08g43780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCATCCAACAATTGTGAACGGTGGTTATTGGCTGAGCAGCACGGCCGGTAAATTACCTGCTTCTAACATTGATGCTACGCTTTACACTCATCTCTTCTGCGGATATGGAGAAATCGACCCCACAAAACATGAAGTGACGATTCCTTCAACAGAAGTGAGTCACTTCTCCGAAAAAGTGAAAGTGAGCAATCCTAGTGTAAAGACTCTGCTGTCCATTAGAGGAGACAGCAAAGTTTTCTCAGACATTGCAAAAGTACCCACAGCACGAGACACGTTCGTCAATTCCTCGATCAAGGCGGCTGTTGATTGTGGGTTTGACGGCCTGGATTTACAGTGGTTGTACCCATCTTCTCCGACTGATATGCAAAATTTTAAAACCCTGATTGTGAAGTGGCGGCAGGCGGCTGTGGACGACGCTAGAAACAACAACCGACCGGAGCGAATCCTGGTGGCTTCCGTGCCGAACCTACCATACATTGAGAAGAATGTTGAGTACCCAGGTGAAGCAATCAAACAAAACCTCGACTGGGTGAACCTTGTTTGTTACGACTTTTACACCCCAGCCGAAGGTTCATCCCCCGATTACACTGGACCATCTTCCGCACTCTTCAACCGCAAGTCCAACTCCTTGTCTGCACGCTTCGGGATCGAATCCTGGACAAAATACGTTCCTCCCAAGAAGATCGTGTTCGGAATCCCTTTCCACGGCTGGGCATGGACACTCGCCAATGCTCTCGAGCACGACGTGTTTTCGAAGGCGACTGGCCTAGCTGCGTCTGGCCAAATGGACTACTCTGAGGTGAAAAAGTTCATAGACGCCCACGCCAGTGTGAGTAAGAACATCCCCAATGACCCCAGATTTGGGATTGCCTACGCCTATGCCGAAACCACCTGGATTGCGTACGAAAGTGAATCCACCATTCCTGTGAAAATTAAATTCGCAAAGCAAAACCAGCTGCTGGGTTACTTTGCTTGGAACATCTCTGGCGACGACGACGTCAACACTCTTGCTAACGCTGCCCAATCCAATTGGCAGTGA

mRNA sequence

ATGTCTCATCCAACAATTGTGAACGGTGGTTATTGGCTGAGCAGCACGGCCGGTAAATTACCTGCTTCTAACATTGATGCTACGCTTTACACTCATCTCTTCTGCGGATATGGAGAAATCGACCCCACAAAACATGAAGTGACGATTCCTTCAACAGAAGTGAGTCACTTCTCCGAAAAAGTGAAAGTGAGCAATCCTAGTGTAAAGACTCTGCTGTCCATTAGAGGAGACAGCAAAGTTTTCTCAGACATTGCAAAAGTACCCACAGCACGAGACACGTTCGTCAATTCCTCGATCAAGGCGGCTGTTGATTGTGGGTTTGACGGCCTGGATTTACAGTGGTTGTACCCATCTTCTCCGACTGATATGCAAAATTTTAAAACCCTGATTGTGAAGTGGCGGCAGGCGGCTGTGGACGACGCTAGAAACAACAACCGACCGGAGCGAATCCTGGTGGCTTCCGTGCCGAACCTACCATACATTGAGAAGAATGTTGAGTACCCAGGTGAAGCAATCAAACAAAACCTCGACTGGGTGAACCTTGTTTGTTACGACTTTTACACCCCAGCCGAAGGTTCATCCCCCGATTACACTGGACCATCTTCCGCACTCTTCAACCGCAAGTCCAACTCCTTGTCTGCACGCTTCGGGATCGAATCCTGGACAAAATACGTTCCTCCCAAGAAGATCGTGTTCGGAATCCCTTTCCACGGCTGGGCATGGACACTCGCCAATGCTCTCGAGCACGACGTGTTTTCGAAGGCGACTGGCCTAGCTGCGTCTGGCCAAATGGACTACTCTGAGGTGAAAAAGTTCATAGACGCCCACGCCAGTGTGAGTAAGAACATCCCCAATGACCCCAGATTTGGGATTGCCTACGCCTATGCCGAAACCACCTGGATTGCGTACGAAAGTGAATCCACCATTCCTGTGAAAATTAAATTCGCAAAGCAAAACCAGCTGCTGGGTTACTTTGCTTGGAACATCTCTGGCGACGACGACGTCAACACTCTTGCTAACGCTGCCCAATCCAATTGGCAGTGA

Coding sequence (CDS)

ATGTCTCATCCAACAATTGTGAACGGTGGTTATTGGCTGAGCAGCACGGCCGGTAAATTACCTGCTTCTAACATTGATGCTACGCTTTACACTCATCTCTTCTGCGGATATGGAGAAATCGACCCCACAAAACATGAAGTGACGATTCCTTCAACAGAAGTGAGTCACTTCTCCGAAAAAGTGAAAGTGAGCAATCCTAGTGTAAAGACTCTGCTGTCCATTAGAGGAGACAGCAAAGTTTTCTCAGACATTGCAAAAGTACCCACAGCACGAGACACGTTCGTCAATTCCTCGATCAAGGCGGCTGTTGATTGTGGGTTTGACGGCCTGGATTTACAGTGGTTGTACCCATCTTCTCCGACTGATATGCAAAATTTTAAAACCCTGATTGTGAAGTGGCGGCAGGCGGCTGTGGACGACGCTAGAAACAACAACCGACCGGAGCGAATCCTGGTGGCTTCCGTGCCGAACCTACCATACATTGAGAAGAATGTTGAGTACCCAGGTGAAGCAATCAAACAAAACCTCGACTGGGTGAACCTTGTTTGTTACGACTTTTACACCCCAGCCGAAGGTTCATCCCCCGATTACACTGGACCATCTTCCGCACTCTTCAACCGCAAGTCCAACTCCTTGTCTGCACGCTTCGGGATCGAATCCTGGACAAAATACGTTCCTCCCAAGAAGATCGTGTTCGGAATCCCTTTCCACGGCTGGGCATGGACACTCGCCAATGCTCTCGAGCACGACGTGTTTTCGAAGGCGACTGGCCTAGCTGCGTCTGGCCAAATGGACTACTCTGAGGTGAAAAAGTTCATAGACGCCCACGCCAGTGTGAGTAAGAACATCCCCAATGACCCCAGATTTGGGATTGCCTACGCCTATGCCGAAACCACCTGGATTGCGTACGAAAGTGAATCCACCATTCCTGTGAAAATTAAATTCGCAAAGCAAAACCAGCTGCTGGGTTACTTTGCTTGGAACATCTCTGGCGACGACGACGTCAACACTCTTGCTAACGCTGCCCAATCCAATTGGCAGTGA

Protein sequence

MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEKVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWAWTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ

Homology

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BLAST of Moc08g43780 vs. NCBI nr
Match: XP_022143253.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.6e-204
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300
           WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

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BLAST of Moc08g43780 vs. NCBI nr
Match: XP_022143018.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 696.4 bits (1796), Expect = 1.2e-196
Identity = 337/347 (97.12%), Postives = 337/347 (97.12%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDXTXHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSX 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKXLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRXSNSLSAXFGIESWTKYVPXKKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300
           WTLANA EHDVFSKATG AASGQMDYSEVKKFIDAHASVS NIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANAXEHDVFSKATGXAASGQMDYSEVKKFIDAHASVSXNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

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BLAST of Moc08g43780 vs. NCBI nr
Match: AAM18075.1 (class V chitinase [Momordica charantia])

HSP 1 Score: 659.8 bits (1701), Expect = 1.3e-185
Identity = 322/347 (92.80%), Postives = 325/347 (93.66%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR+SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300
           WTLANA EHD+FSKATG +    MDYSEVK    A      NIPNDPRFGIAYAY ETTW
Sbjct: 241 WTLANAAEHDMFSKATG-SCVWPMDYSEVKSHRRAR-QCEYNIPNDPRFGIAYAYVETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           I YESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IGYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 345

[Close]

BLAST of Moc08g43780 vs. NCBI nr
Match: XP_022143252.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 607.4 bits (1565), Expect = 7.5e-170
Identity = 298/355 (83.94%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLS+TAGKLPASNIDATLYTHLFCGY EI+ T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VK+SNP+VKTLLSI GDSKVFSDIAKVPTARDTF+ SSIKAAV+ GFDGLDLQWLYPSSP
Sbjct: 61  VKMSNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNF+TLI KWRQA VDDAR  NR  RILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFETLIGKWRQAVVDDARTYNRAARILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSS   TGPSSALFNR + SLSA FGIESWTKYVP  KIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLSACFGIESWTKYVPATKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAAS---------GQMDYSEVKKFIDAHASVSKNIPNDPRFGI 300
           W LA+  E+DVFSKA+G AAS         GQM+YSEVKKFI A+A   KNIPNDPRFGI
Sbjct: 241 WQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPRFGI 300

Query: 301 AYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           AYAYA  TWIAYESESTIPVKI+ AKQNQLLGYFAWNISGDDDVNTL NAA++NW
Sbjct: 301 AYAYANATWIAYESESTIPVKIELAKQNQLLGYFAWNISGDDDVNTLYNAAKTNW 355

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BLAST of Moc08g43780 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 431.8 bits (1109), Expect = 5.6e-117
Identity = 218/359 (60.72%), Postives = 270/359 (75.21%), Query Frame = 0


Query: 2   SHPT-IVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTE---VSHF 61
           S PT  VNGGYWLSST  +LP SNIDA L+THLFCG+ EID   +EVT+P+++   ++ F
Sbjct: 5   SKPTSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQF 64

Query: 62  SEKVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYP 121
           S  V++SNP VKTLLSIRGD  VF+ +A  PT RD F+ SSI+ A    F+GLDLQWLYP
Sbjct: 65  SSTVRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYP 124

Query: 122 SSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLD 181
           SS  +MQNF+ L+  WRQAAVDDA+  +RP+ ILVASV NLPY+E ++ YP E IKQNLD
Sbjct: 125 SSEEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLD 184

Query: 182 WVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTK---YVPPKKIVFGI 241
           WVNL+ YDFYTPA  SSP  TGPSSAL NR + SLSA FGI++W +    +P K+IVFGI
Sbjct: 185 WVNLISYDFYTPA--SSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGI 244

Query: 242 PFHGWAWTLANALEHDVFSKATG-------LAASGQMDYSEVKKFIDAHASVSKNIPNDP 301
           PFHGWAW LAN  +HDVFSKA G       ++ +GQ+D S VK+FI  +   ++ + +D 
Sbjct: 245 PFHGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNH--AQYVKDDS 304

Query: 302 RFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           R+ I+YAYA TTWIAYESE TIP KI  AK +++LGYFAWNI+ DD+VNTLANAAQ+NW
Sbjct: 305 RYSISYAYAGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

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BLAST of Moc08g43780 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 143/353 (40.51%), Postives = 210/353 (59.49%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPST---EVSHFSEKV 64
           T+V   YW    A + P ++ID++L+THLFC + +++   ++VT+ S    + S F++ V
Sbjct: 26  TVVKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTV 85

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           +  NPSVKTLLSI G   D   ++ +A  PT+R +F++SSI+ A   GF GLDL W YPS
Sbjct: 86  QRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPS 145

Query: 125 SPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASV--PNLPYIEKNVEYPGEAIKQNL 184
           S T+M NF TL+ +WR A V +A ++ +P  +L A+V   N  Y   +V YP  A+  +L
Sbjct: 146 SATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSL 205

Query: 185 DWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY-VPPKKIVFGIP 244
           DWVNL+ YDFY P        TGP +ALF+  +   S   G  SW +  +P KK V G P
Sbjct: 206 DWVNLMAYDFYGPGWSR---VTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFP 265

Query: 245 FHGWAWTLANALEHDVFSKATGLAAS--GQMDYSEVKKFIDAHASVSKNIPNDPRFGIAY 304
           ++G+AW L NA  H  ++  TG A S  G + Y +++KFI  + + +  + N    G  Y
Sbjct: 266 YYGYAWRLTNANSHSYYAPTTGAAISPDGSIGYGQIRKFIVDNGATT--VYNSTVVG-DY 325

Query: 305 AYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
            YA T WI Y+   +I  K+++AKQ  LLGYF+W++ G DD + L+ AA   W
Sbjct: 326 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHV-GADDNSGLSRAASQAW 366

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BLAST of Moc08g43780 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 9.5e-51
Identity = 124/364 (34.07%), Postives = 193/364 (53.02%), Query Frame = 0


Query: 2   SHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTK---------HEVTIPST 61
           S  T V G YW+ +     P S+ID +L+TH+F  Y  + P K          + T  +T
Sbjct: 32  SSTTRVKGIYWIENPL--FPPSSIDTSLFTHIF--YAFVSPNKFTYKLEEEEEDSTTVAT 91

Query: 62  EVSHFSEKVKVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDG 121
            ++ F+   K   P + TLLSI G   +S +F+ IA  PTAR TF+NS+I+ A   GFDG
Sbjct: 92  SLTTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDG 151

Query: 122 LDLQWLYPSSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASV---PNLPYIEKNVE 181
           +D  W +P++  +M +   L+ +WR+A  D+  + +RP  +L A+V    N     +   
Sbjct: 152 IDFDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRM 211

Query: 182 YPGEAIKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTK-YV 241
           YP ++I +NLDWVN++ YD      GS  + TG  S +F+ KSN +S   G+ SW +  V
Sbjct: 212 YPVDSINKNLDWVNVMSYDL----RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWIRGGV 271

Query: 242 PPKKIVFGIPFHGWAWTLANALEHDVFSK--ATGLAASGQMDYSEVKKFIDAHASVSKNI 301
            P+KIV G+P +G +W L +   H + +     G    G M Y +V   +D +  +   +
Sbjct: 272 APEKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGPGVDGGMAYFQV---VDFNKQMGAKV 331

Query: 302 PNDPRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQ 348
             D   G  Y+Y+ +TWI Y+   T+ VK+ FA+  +L GYF W  +G D  +   + A 
Sbjct: 332 VYDKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFW-AAGYDTSDWKVSTAS 382

[Close]

BLAST of Moc08g43780 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 8.6e-44
Identity = 112/356 (31.46%), Postives = 186/356 (52.25%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEV---SHFSEKV 64
           T V G YWL +     P + +D +L+TH+F  +   +   +++ I S+++   + F++  
Sbjct: 33  TRVKGIYWLENPF--FPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTF 92

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           K  +P   TL SI G   +S + + IA  P A   F+NS+I  A   GFDG+DL W +P 
Sbjct: 93  KTKSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPK 152

Query: 125 SPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASV---PNLPYIEKNVEYPGEAIKQN 184
           +  +M +   ++ +WR+A  D+     RP  +L A+V    N     +   YP  +I +N
Sbjct: 153 NTKEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINEN 212

Query: 185 LDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWT-KYVPPKKIVFGI 244
           LDWVN++ Y+      G   + TG  S  F+ KSN +S   G+ SW    V P+K+V G+
Sbjct: 213 LDWVNVMSYEL----RGPRSNKTGAPSGTFDPKSN-VSVVSGLLSWIHSGVVPEKLVMGM 272

Query: 245 PFHGWAWTLANALEHDVF--SKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIA 304
           P +G +W L +   H +   S  +G   +G M Y +V   +D +   S  +  D      
Sbjct: 273 PLYGKSWKLRDPNVHGIGAPSVGSGPGVNGLMAYFQV---LDFNRQKSAKVEYDVDTASV 332

Query: 305 YAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVN-TLANAAQSNWQ 348
           Y+Y+ +TWI Y++  T+ +K+ FA+  +L GYF W ++G D ++  +A  A   W+
Sbjct: 333 YSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFW-VAGLDTLDWKIATQASKAWK 377

[Close]

BLAST of Moc08g43780 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-43
Identity = 114/354 (32.20%), Postives = 185/354 (52.26%), Query Frame = 0


Query: 7   VNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEV---SHFSEKVKV 66
           V   YW          S ID   +TH+   + + +P   ++ I  + +    +F + ++ 
Sbjct: 40  VRSAYW--PAGDDFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 67  SNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 126
            +P VKTLLSI G   +S +FS+IA     R+ F+NS+I+ A    FDG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 127 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASV---PNLPYIEKN--VEYPGEAIKQN 186
            DM N   L  +W  A   +A+   +P  +L ++V     +  I K+    YP +AI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 187 LDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY-VPPKKIVFGI 246
           LDW + +C+D++    G+  + T  ++AL++ KS  +S  FG+ SW K  V P+K+V G+
Sbjct: 220 LDWASPMCFDYH----GTWDNNTDFNAALYDSKS-EISTNFGLHSWIKSGVRPEKLVMGL 279

Query: 247 PFHGWAWTLANALEHDVFSKATGLA--ASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIA 306
             +G AW L +   + V ++A G A    G M+Y+E+ KF   +     N+  D      
Sbjct: 280 ALYGRAWELKDPNVNGVGAEAVGPATDTDGSMNYNEILKF---NKQSGANVVYDKVAISF 339

Query: 307 YAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           Y+YA TTWI Y+   +I  K++FAK   L GYF W +  D D  +++  A + W
Sbjct: 340 YSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDW-SISKQASNAW 382

[Close]

BLAST of Moc08g43780 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 174.1 bits (440), Expect = 2.8e-42
Identity = 115/356 (32.30%), Postives = 178/356 (50.00%), Query Frame = 0


Query: 7   VNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEKVKVSN- 66
           V   YW          S+ID   +TH++  + +  P    + I  +      +   + N 
Sbjct: 55  VRAAYW--PAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNI 114

Query: 67  -PSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSPT 126
            P V TLLSI G   ++ +FS++A     R +F+NS+I  A    F+GLDL W +P    
Sbjct: 115 RPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDEK 174

Query: 127 DMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNV--------EYPGEAIK 186
           DM N   L+ +W +A V +A N +R  R+L+ S     Y    +         YP  AI+
Sbjct: 175 DMSNLALLLKEWYKALVVEA-NTSRKSRLLLTSA---VYFNSTISLIGNGPRSYPVRAIR 234

Query: 187 QNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWT-KYVPPKKIVF 246
           + LDW + +C+D+     G+  + TG ++AL++  SN +S ++GI SW    VP +K+V 
Sbjct: 235 KYLDWASPMCFDY----NGAWANETGFNAALYDPNSN-ISTKYGIGSWIGSGVPAEKLVM 294

Query: 247 GIPFHGWAWTLANALEHDVFSKATGLA--ASGQMDYSEVKKFIDAHASVSKNIPNDPRFG 306
           G+P +G AW L +  +H V +KA G A    G MDY E+  F   +      +  D    
Sbjct: 295 GLPLYGRAWELKDPNDHGVGAKAVGPAVDTDGSMDYDEILVF---NKDTGAKVVYDEVAV 354

Query: 307 IAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
             Y+Y+ TTWI Y+   +I  K++FA+   L GYF W I  D D  T++  A + W
Sbjct: 355 SFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQASNAW 395

[Close]

BLAST of Moc08g43780 vs. ExPASy TrEMBL
Match: A0A6J1CQ93 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.2e-204
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300
           WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

[Close]

BLAST of Moc08g43780 vs. ExPASy TrEMBL
Match: A0A6J1CPP6 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013006 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 5.9e-197
Identity = 337/347 (97.12%), Postives = 337/347 (97.12%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDXTXHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSX 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKXLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRXSNSLSAXFGIESWTKYVPXKKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300
           WTLANA EHDVFSKATG AASGQMDYSEVKKFIDAHASVS NIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANAXEHDVFSKATGXAASGQMDYSEVKKFIDAHASVSXNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

[Close]

BLAST of Moc08g43780 vs. ExPASy TrEMBL
Match: Q8S2V9 (Class V chitinase OS=Momordica charantia OX=3673 GN=Chi5 PE=2 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 6.1e-186
Identity = 322/347 (92.80%), Postives = 325/347 (93.66%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR+SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300
           WTLANA EHD+FSKATG +    MDYSEVK    A      NIPNDPRFGIAYAY ETTW
Sbjct: 241 WTLANAAEHDMFSKATG-SCVWPMDYSEVKSHRRAR-QCEYNIPNDPRFGIAYAYVETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           I YESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IGYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 345

[Close]

BLAST of Moc08g43780 vs. ExPASy TrEMBL
Match: A0A6J1CQ95 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.6e-170
Identity = 298/355 (83.94%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLS+TAGKLPASNIDATLYTHLFCGY EI+ T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120
           VK+SNP+VKTLLSI GDSKVFSDIAKVPTARDTF+ SSIKAAV+ GFDGLDLQWLYPSSP
Sbjct: 61  VKMSNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNF+TLI KWRQA VDDAR  NR  RILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFETLIGKWRQAVVDDARTYNRAARILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSS   TGPSSALFNR + SLSA FGIESWTKYVP  KIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLSACFGIESWTKYVPATKIVFGIPFHGWA 240

Query: 241 WTLANALEHDVFSKATGLAAS---------GQMDYSEVKKFIDAHASVSKNIPNDPRFGI 300
           W LA+  E+DVFSKA+G AAS         GQM+YSEVKKFI A+A   KNIPNDPRFGI
Sbjct: 241 WQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPRFGI 300

Query: 301 AYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           AYAYA  TWIAYESESTIPVKI+ AKQNQLLGYFAWNISGDDDVNTL NAA++NW
Sbjct: 301 AYAYANATWIAYESESTIPVKIELAKQNQLLGYFAWNISGDDDVNTLYNAAKTNW 355

[Close]

BLAST of Moc08g43780 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.7e-117
Identity = 218/359 (60.72%), Postives = 270/359 (75.21%), Query Frame = 0


Query: 2   SHPT-IVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTE---VSHF 61
           S PT  VNGGYWLSST  +LP SNIDA L+THLFCG+ EID   +EVT+P+++   ++ F
Sbjct: 5   SKPTSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQF 64

Query: 62  SEKVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYP 121
           S  V++SNP VKTLLSIRGD  VF+ +A  PT RD F+ SSI+ A    F+GLDLQWLYP
Sbjct: 65  SSTVRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYP 124

Query: 122 SSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLD 181
           SS  +MQNF+ L+  WRQAAVDDA+  +RP+ ILVASV NLPY+E ++ YP E IKQNLD
Sbjct: 125 SSEEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLD 184

Query: 182 WVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTK---YVPPKKIVFGI 241
           WVNL+ YDFYTPA  SSP  TGPSSAL NR + SLSA FGI++W +    +P K+IVFGI
Sbjct: 185 WVNLISYDFYTPA--SSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGI 244

Query: 242 PFHGWAWTLANALEHDVFSKATG-------LAASGQMDYSEVKKFIDAHASVSKNIPNDP 301
           PFHGWAW LAN  +HDVFSKA G       ++ +GQ+D S VK+FI  +   ++ + +D 
Sbjct: 245 PFHGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNH--AQYVKDDS 304

Query: 302 RFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           R+ I+YAYA TTWIAYESE TIP KI  AK +++LGYFAWNI+ DD+VNTLANAAQ+NW
Sbjct: 305 RYSISYAYAGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

[Close]

BLAST of Moc08g43780 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-69
Identity = 143/353 (40.51%), Postives = 210/353 (59.49%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPST---EVSHFSEKV 64
           T+V   YW    A + P ++ID++L+THLFC + +++   ++VT+ S    + S F++ V
Sbjct: 26  TVVKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTV 85

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           +  NPSVKTLLSI G   D   ++ +A  PT+R +F++SSI+ A   GF GLDL W YPS
Sbjct: 86  QRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPS 145

Query: 125 SPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASV--PNLPYIEKNVEYPGEAIKQNL 184
           S T+M NF TL+ +WR A V +A ++ +P  +L A+V   N  Y   +V YP  A+  +L
Sbjct: 146 SATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSL 205

Query: 185 DWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY-VPPKKIVFGIP 244
           DWVNL+ YDFY P        TGP +ALF+  +   S   G  SW +  +P KK V G P
Sbjct: 206 DWVNLMAYDFYGPGWSR---VTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFP 265

Query: 245 FHGWAWTLANALEHDVFSKATGLAAS--GQMDYSEVKKFIDAHASVSKNIPNDPRFGIAY 304
           ++G+AW L NA  H  ++  TG A S  G + Y +++KFI  + + +  + N    G  Y
Sbjct: 266 YYGYAWRLTNANSHSYYAPTTGAAISPDGSIGYGQIRKFIVDNGATT--VYNSTVVG-DY 325

Query: 305 AYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
            YA T WI Y+   +I  K+++AKQ  LLGYF+W++ G DD + L+ AA   W
Sbjct: 326 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHV-GADDNSGLSRAASQAW 366

[Close]

BLAST of Moc08g43780 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 243.8 bits (621), Expect = 2.0e-64
Identity = 134/343 (39.07%), Postives = 204/343 (59.48%), Query Frame = 0


Query: 2   SHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPS---TEVSHFS 61
           S  T+V   YW + +  + P + ID++L+THLFC + +I+   ++V + S    + S F+
Sbjct: 21  SAQTVVKATYWFAES--ESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFT 80

Query: 62  EKVKVSNPSVKTLLSIRGD---SKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWL 121
           + V+  NP+VKTLLSI GD   +  F+ +A  PT+R  F++SSIK A  CGF GLDL W 
Sbjct: 81  QTVRRRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWK 140

Query: 122 YPSSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQN 181
           YPS  T+M NF  L+ +WR A   +AR++ +P  +L A+V    Y   +V +P  A+  +
Sbjct: 141 YPSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAV-FYSYSYYSVLHPVNAVADS 200

Query: 182 LDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY-VPPKKIVFGI 241
           LDWVNLV YDFY   E  S   T   + L++  +   S   G+ +WT+  +P KK V G 
Sbjct: 201 LDWVNLVAYDFY---ESGSSRVTCSPAPLYDPITTGPSGDAGVRAWTQAGLPAKKAVLGF 260

Query: 242 PFHGWAWTLANALEHDVFSKATGLAAS--GQMDYSEVKKFI-DAHASVSKNIPNDPRFGI 301
           P +G+AW L +A  H+ ++ ++G A S  G + Y ++++FI D  A++  N         
Sbjct: 261 PLYGYAWCLTDAKNHNYYANSSGPAISPDGSIGYDQIRRFIVDNKATMVYN----SNLVQ 320

Query: 302 AYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDD 335
            Y YA+ TWI Y+   +I +K+K+AKQ  LLGYF+W+I  DD+
Sbjct: 321 NYCYAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

[Close]

BLAST of Moc08g43780 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 232.3 bits (591), Expect = 6.1e-61
Identity = 131/346 (37.86%), Postives = 198/346 (57.23%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPS---TEVSHF 60
           MS   +V   YW  +T    PA++ID++L+THLFC + +++   +E+TI +        F
Sbjct: 1   MSESDVVKASYWFPAT--DFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAF 60

Query: 61  SEKVKVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQW 120
           +E V+  NP VKTLLSI G   D   F+ +A  P +R +F+ S+I  A   GF GLDL W
Sbjct: 61  TETVQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDW 120

Query: 121 LYPSSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKN---VEYPGEA 180
            YP +  +M +F  L+ +WR A   ++ ++     IL A+V    Y   N   V YP  A
Sbjct: 121 EYPRNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLA 180

Query: 181 IKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY-VPPKKI 240
           I  +LDW+NL+ YDFY P   +    TGP ++L+   ++  S   G+  WT+  +P KK 
Sbjct: 181 ISNSLDWINLMAYDFYGPGWST---VTGPPASLY-LPTDGRSGDSGVRDWTEAGLPAKKA 240

Query: 241 VFGIPFHGWAWTLANALEHDVFSKATGLAAS--GQMDYSEVKKFIDAHASVSKNIPNDPR 300
           V G P++GWAWTLA+   +   +  TG A S  G++ Y +++ +I  + +    + +D  
Sbjct: 241 VLGFPYYGWAWTLADPDVNGYDANTTGPAISDDGEISYRQLQTWIVDNGAT--KVHDDMM 300

Query: 301 FGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDD 335
            G  Y YA TTWI Y+SE +I  K+ +AKQ  LLGYF+W++ GDD+
Sbjct: 301 VG-DYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

[Close]

BLAST of Moc08g43780 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-60
Identity = 138/362 (38.12%), Postives = 198/362 (54.70%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGK--------LPASNIDATLYTHLFCGYGEIDPTKHEVTIP---STE 64
           +IV   YW      +         P+S ID+TL+THLFC + ++D + HEVTI    S +
Sbjct: 12  SIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQ 71

Query: 65  VSHFSEKVKVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGL 124
            S F+E VK  N  V+TLLSI G   D  V + +A     R  F++SSI  A    F GL
Sbjct: 72  FSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGL 131

Query: 125 DLQWLYPSSPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGE 184
           DL W YPS+  +M NF  L+ +WR A V+++   N+   +L A+V   P  +  VEYP +
Sbjct: 132 DLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYD-GVEYPVK 191

Query: 185 AIKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSN--SLSARFGIESW--TKYVP 244
           AI  NLD+VN++ YDFY P  G SP  TGP +ALF+  SN    S   G+  W     +P
Sbjct: 192 AIADNLDFVNIMAYDFYGP--GWSP-VTGPPAALFHDPSNPAGRSGNSGLRKWLDEAKLP 251

Query: 245 PKKIVFGIPFHGWAWTLANALE--HDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIP 304
           PKK V G P+ GWAWTL +A    +D  +    ++  G + Y++++ +I  + + +    
Sbjct: 252 PKKAVLGFPYCGWAWTLEDAENNGYDAATDGAAISPDGSITYAKIRNYIVDNGAATF--- 311

Query: 305 NDPRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQS 347
           +DP     Y Y   TWI Y+   +I  K+K+AK   LLGYF+W++  D +   L+ AA  
Sbjct: 312 HDPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAASI 365

[Close]

BLAST of Moc08g43780 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 226.1 bits (575), Expect = 4.4e-59
Identity = 131/340 (38.53%), Postives = 192/340 (56.47%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIP---STEVSHFSEKV 64
           +IV   YW+       PA NID+T +THLFC + ++D + HEVTI    S +VS F+  V
Sbjct: 12  SIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTV 71

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           K  N  V+TLLSI G   D  V + +A     R  F++SSI  A    F GLDL W YPS
Sbjct: 72  KDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPS 131

Query: 125 SPTDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLP--YIEKNVEYPGEAIKQNL 184
           +  +M NF  L+ +WR A V+++   N+   +L A+V   P  Y E   EYP +AI  NL
Sbjct: 132 NDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGE---EYPVQAIADNL 191

Query: 185 DWVNLVCYDFYTPAEGSSPDYTGPSSALFNRKSNS-LSARFGIESWTK-YVPPKKIVFGI 244
           D+VN++ YDFY P  G SP  TGP +ALF+  + +  S   G+  W +  +P KK V G 
Sbjct: 192 DFVNIMAYDFYGP--GWSP-VTGPPAALFDPSNPAGRSGDSGLSKWLEAKLPAKKAVLGF 251

Query: 245 PFHGWAWTLANALE--HDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIA 304
            + GWAWTL +A    +D  +    +++ G + Y++++ +I  + + +    +DP     
Sbjct: 252 SYCGWAWTLEDAENNGYDAATDGAAISSDGSITYAKIRNYIIDNGAATF---HDPAVIGF 311

Query: 305 YAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGD 333
           Y Y  TTWI Y+   +I  K+++AK   LLGYF+W++  D
Sbjct: 312 YCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

The following BLAST results are available for this feature:

BLAST of Moc08g43780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143253.1	4.6e-204	100.00	chitotriosidase-1-like [Momordica charantia]	[more]
XP_022143018.1	1.2e-196	97.12	acidic mammalian chitinase-like [Momordica charantia]	[more]
AAM18075.1	1.3e-185	92.80	class V chitinase [Momordica charantia]	[more]
XP_022143252.1	7.5e-170	83.94	acidic mammalian chitinase-like [Momordica charantia]	[more]
XP_022159350.1	5.6e-117	60.72	chitinase-3-like protein 2 [Momordica charantia]	[more]

BLAST of Moc08g43780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	5.0e-68	40.51	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
U5N4E3	9.5e-51	34.07	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A072UR65	8.6e-44	31.46	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
A0A072VEP0	1.9e-43	32.20	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]
A0A1B1J8Z2	2.8e-42	32.30	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]

BLAST of Moc08g43780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQ93	2.2e-204	100.00	chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1	[more]
A0A6J1CPP6	5.9e-197	97.12	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013006 P...	[more]
Q8S2V9	6.1e-186	92.80	Class V chitinase OS=Momordica charantia OX=3673 GN=Chi5 PE=2 SV=1	[more]
A0A6J1CQ95	3.6e-170	83.94	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 P...	[more]
A0A6J1DYG4	2.7e-117	60.72	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]

BLAST of Moc08g43780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	3.6e-69	40.51	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	2.0e-64	39.07	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	6.1e-61	37.86	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	1.8e-60	38.12	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	4.4e-59	38.53	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 6..332 e-value: 1.9E-66 score: 236.7
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 9..332 e-value: 2.5E-58 score: 198.3
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 6..347 score: 46.92128
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..333 e-value: 1.2E-79 score: 270.1
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 5..341
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 5..341
None	No IPR available	CDD	cd02879	GH18_plant_chitinase_class_V	coord: 4..338 e-value: 7.80316E-141 score: 399.432
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 236..303 e-value: 1.2E-79 score: 270.1
IPR029070	Chitinase insertion domain superfamily	SUPERFAMILY	54556	Chitinase insertion domain	coord: 237..303
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 7..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43780.1	Moc08g43780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0016787	hydrolase activity