Moc08g43740 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43740
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	chitinase-3-like protein 2
Location	chr8: 33616883 .. 33617968 (-)
RNA-Seq Expression	Moc08g43740
Synteny	Moc08g43740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAAATTTTGTGAATGGTGGCTACTGGCTGAGCAACGAGGCTGCGCAATTTCCTGCTGACAAGATGGATGCTACGCTTTACACCCATCTGTTCTGTGGATATGCAGAAATCGACATCCAAAGTTATGAGGTGATAGTTCCTTTTGGGCAATATAACATAGTCAATGCATTCTCTTCCCAGGTGAAATTGAGCAATCCAGATGTGAAAACTATGCTATCTATTAGGGGAGAGAAAGAAATATTCTCTCAAATGGCAGGCAACCGCCTAACACGCGAAGTATTTATCAAATCCTCGATCAAGGCAGCAGTTGCTGGCAACTTTAACGGCTTGGATTTATGCTGGCTATACCCGTCTACACAAGATGATATGGAAAACTTCGATTACTTGATTACTGAATGGCGCCAGGTCGCTGAGGATGACGCTAGAGACAACAACCGAAAAGCGTCGTGGCTCCTTGTGGCTTCGGTGCCGAACGCACCCTATGTTGAGCCCAATATACATGTTGTGTACCCAGCTACAGCAATCAGCCAGAACCTAGACTGGGTCAATCTTATTTCTTACGACTTTTACACCCCAACTTCATGCCCTGGTTACACTGCACCATCCTCCGCATTCAACAACCACAAAGCCAACCTCTTGTCCGCACATTTCGGTATCGATTCGTGGAGTAAAATCCTTGGCGATTTGCCTTTTGATAAGATTGTGTTTGGAATTCCTTTCCACGGGTGGGCCTGGAAGCTCACCAACCCTCTCCAGCACAACGTGTTTTCCAAGGCCGATGGCCCGACTGAGTCTACTGGAGAATATGAGATTGCCCTAGATGGTAAAATTGTCTACTACGAGGTCCAGAACTTCATACATGAGACTCAAGCTGAAGTTGTCCCAAATGACCGGAAATTTGTGATTGCCTACGCCTTTAATGCCACTACCACCTGGATTGCCTTCGAGAGTAAAGACACCATTTCTCAGAAAGTTAACTTAGCCAGGTCAATGATGGCGCTGGGTTACTTTGCTTCCAACGTTGCTGCTGACGATGAACTCTACACTCTGGCCACCGCTGCCAAATCCAGTTGGTCCTCCTAA

mRNA sequence

ATGACAAATTTTGTGAATGGTGGCTACTGGCTGAGCAACGAGGCTGCGCAATTTCCTGCTGACAAGATGGATGCTACGCTTTACACCCATCTGTTCTGTGGATATGCAGAAATCGACATCCAAAGTTATGAGGTGATAGTTCCTTTTGGGCAATATAACATAGTCAATGCATTCTCTTCCCAGGTGAAATTGAGCAATCCAGATGTGAAAACTATGCTATCTATTAGGGGAGAGAAAGAAATATTCTCTCAAATGGCAGGCAACCGCCTAACACGCGAAGTATTTATCAAATCCTCGATCAAGGCAGCAGTTGCTGGCAACTTTAACGGCTTGGATTTATGCTGGCTATACCCGTCTACACAAGATGATATGGAAAACTTCGATTACTTGATTACTGAATGGCGCCAGGTCGCTGAGGATGACGCTAGAGACAACAACCGAAAAGCGTCGTGGCTCCTTGTGGCTTCGGTGCCGAACGCACCCTATGTTGAGCCCAATATACATGTTGTGTACCCAGCTACAGCAATCAGCCAGAACCTAGACTGGGTCAATCTTATTTCTTACGACTTTTACACCCCAACTTCATGCCCTGGTTACACTGCACCATCCTCCGCATTCAACAACCACAAAGCCAACCTCTTGTCCGCACATTTCGGTATCGATTCGTGGAGTAAAATCCTTGGCGATTTGCCTTTTGATAAGATTGTGTTTGGAATTCCTTTCCACGGGTGGGCCTGGAAGCTCACCAACCCTCTCCAGCACAACGTGTTTTCCAAGGCCGATGGCCCGACTGAGTCTACTGGAGAATATGAGATTGCCCTAGATGGTAAAATTGTCTACTACGAGGTCCAGAACTTCATACATGAGACTCAAGCTGAAGTTGTCCCAAATGACCGGAAATTTGTGATTGCCTACGCCTTTAATGCCACTACCACCTGGATTGCCTTCGAGAGTAAAGACACCATTTCTCAGAAAGTTAACTTAGCCAGGTCAATGATGGCGCTGGGTTACTTTGCTTCCAACGTTGCTGCTGACGATGAACTCTACACTCTGGCCACCGCTGCCAAATCCAGTTGGTCCTCCTAA

Coding sequence (CDS)

ATGACAAATTTTGTGAATGGTGGCTACTGGCTGAGCAACGAGGCTGCGCAATTTCCTGCTGACAAGATGGATGCTACGCTTTACACCCATCTGTTCTGTGGATATGCAGAAATCGACATCCAAAGTTATGAGGTGATAGTTCCTTTTGGGCAATATAACATAGTCAATGCATTCTCTTCCCAGGTGAAATTGAGCAATCCAGATGTGAAAACTATGCTATCTATTAGGGGAGAGAAAGAAATATTCTCTCAAATGGCAGGCAACCGCCTAACACGCGAAGTATTTATCAAATCCTCGATCAAGGCAGCAGTTGCTGGCAACTTTAACGGCTTGGATTTATGCTGGCTATACCCGTCTACACAAGATGATATGGAAAACTTCGATTACTTGATTACTGAATGGCGCCAGGTCGCTGAGGATGACGCTAGAGACAACAACCGAAAAGCGTCGTGGCTCCTTGTGGCTTCGGTGCCGAACGCACCCTATGTTGAGCCCAATATACATGTTGTGTACCCAGCTACAGCAATCAGCCAGAACCTAGACTGGGTCAATCTTATTTCTTACGACTTTTACACCCCAACTTCATGCCCTGGTTACACTGCACCATCCTCCGCATTCAACAACCACAAAGCCAACCTCTTGTCCGCACATTTCGGTATCGATTCGTGGAGTAAAATCCTTGGCGATTTGCCTTTTGATAAGATTGTGTTTGGAATTCCTTTCCACGGGTGGGCCTGGAAGCTCACCAACCCTCTCCAGCACAACGTGTTTTCCAAGGCCGATGGCCCGACTGAGTCTACTGGAGAATATGAGATTGCCCTAGATGGTAAAATTGTCTACTACGAGGTCCAGAACTTCATACATGAGACTCAAGCTGAAGTTGTCCCAAATGACCGGAAATTTGTGATTGCCTACGCCTTTAATGCCACTACCACCTGGATTGCCTTCGAGAGTAAAGACACCATTTCTCAGAAAGTTAACTTAGCCAGGTCAATGATGGCGCTGGGTTACTTTGCTTCCAACGTTGCTGCTGACGATGAACTCTACACTCTGGCCACCGCTGCCAAATCCAGTTGGTCCTCCTAA

Protein sequence

MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWSS

Homology

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BLAST of Moc08g43740 vs. NCBI nr
Match: XP_022143190.1 (probable chitinase 10 [Momordica charantia])

HSP 1 Score: 744.6 bits (1921), Expect = 4.1e-211
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0


Query: 1   MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS 60
           MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS
Sbjct: 2   MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS 61

Query: 61  QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST 120
           QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST
Sbjct: 62  QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST 121

Query: 121 QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL 180
           QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL
Sbjct: 122 QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL 181

Query: 181 DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP 240
           DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP
Sbjct: 182 DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP 241

Query: 241 FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK 300
           FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK
Sbjct: 242 FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK 301

Query: 301 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS 360
           FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS
Sbjct: 302 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS 361

Query: 361 S 362
           S
Sbjct: 362 S 362

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BLAST of Moc08g43740 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 406.8 bits (1044), Expect = 2.0e-109
Identity = 202/359 (56.27%), Postives = 263/359 (73.26%), Query Frame = 0


Query: 2   TNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQ 61
           T+ VNGGYWLS+   Q P   +DA L+THLFCG+AEID  SYEV VP  Q  ++N FSS 
Sbjct: 8   TSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSST 67

Query: 62  VKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 121
           V++SNPDVKT+LSIRG++++F++MA     R+ FI+SSI+ A  G FNGLDL WLYPS++
Sbjct: 68  VRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSE 127

Query: 122 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLD 181
           ++M+NF+ L+  WRQ A DDA+  +R   W+LVASV N PYVE +I  VYP   I QNLD
Sbjct: 128 EEMQNFEILLGAWRQAAVDDAKTYHR-PQWILVASVSNLPYVESSI--VYPVEEIKQNLD 187

Query: 182 WVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPF 241
           WVNLISYDFYTP S P  T PSSA +N  A  LSA+FGID+W + L +LP  +IVFGIPF
Sbjct: 188 WVNLISYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPF 247

Query: 242 HGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRKF 301
           HGWAWKL NP +H+VFSKA GP +  G+  I+ +G+I    V+ FI +  A+ V +D ++
Sbjct: 248 HGWAWKLANPDKHDVFSKALGPAK--GDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRY 307

Query: 302 VIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS 361
            I+YA+ A TTWIA+ES+ TI  K++ A+S   LGYFA N+AADDE+ TLA AA+++WS
Sbjct: 308 SISYAY-AGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNWS 360

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BLAST of Moc08g43740 vs. NCBI nr
Match: XP_022143253.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 384.0 bits (985), Expect = 1.4e-102
Identity = 200/359 (55.71%), Postives = 251/359 (69.92%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           VNGGYWLS+ A + PA  +DATLYTHLFCGY EID   +EV +P  +   V+ FS +VK+
Sbjct: 7   VNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTE---VSHFSEKVKV 66

Query: 65  SNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQDDM 124
           SNP VKT+LSIRG+ ++FS +A     R+ F+ SSIKAAV   F+GLDL WLYPS+  DM
Sbjct: 67  SNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSPTDM 126

Query: 125 ENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLDWVN 184
           +NF  LI +WRQ A DDAR+NNR    +LVASVPN PY+E N+   YP  AI QNLDWVN
Sbjct: 127 QNFKTLIVKWRQAAVDDARNNNRPER-ILVASVPNLPYIEKNVE--YPGEAIKQNLDWVN 186

Query: 185 LISYDFYTPT--SCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPFH 244
           L+ YDFYTP   S P YT PSSA  N K+N LSA FGI+SW+K    +P  KIVFGIPFH
Sbjct: 187 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY---VPPKKIVFGIPFH 246

Query: 245 GWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFI--HETQAEVVPNDRK 304
           GWAW L N L+H+VFSKA G         +A  G++ Y EV+ FI  H + ++ +PND +
Sbjct: 247 GWAWTLANALEHDVFSKATG---------LAASGQMDYSEVKKFIDAHASVSKNIPNDPR 306

Query: 305 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSW 360
           F IAYA+ A TTWIA+ES+ TI  K+  A+    LGYFA N++ DD++ TLA AA+S+W
Sbjct: 307 FGIAYAY-AETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 346

[Close]

BLAST of Moc08g43740 vs. NCBI nr
Match: XP_022143252.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 382.9 bits (982), Expect = 3.1e-102
Identity = 199/359 (55.43%), Postives = 252/359 (70.19%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           VNGGYWLSN A + PA  +DATLYTHLFCGYAEI+  S+EV +P  +   V+ FS +VK+
Sbjct: 7   VNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTE---VSHFSEKVKM 66

Query: 65  SNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQDDM 124
           SNP+VKT+LSI G+ ++FS +A     R+ FIKSSIKAAV   F+GLDL WLYPS+  DM
Sbjct: 67  SNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSSPTDM 126

Query: 125 ENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLDWVN 184
           +NF+ LI +WRQ   DDAR  NR A+ +LVASVPN PY+E N+   YP  AI QNLDWVN
Sbjct: 127 QNFETLIGKWRQAVVDDARTYNR-AARILVASVPNLPYIEKNVE--YPGEAIKQNLDWVN 186

Query: 185 LISYDFYTPT--SCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPFH 244
           L+ YDFYTP   S  G T PSSA  N     LSA FGI+SW+K    +P  KIVFGIPFH
Sbjct: 187 LVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLSACFGIESWTKY---VPATKIVFGIPFH 246

Query: 245 GWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFI--HETQAEVVPNDRK 304
           GWAW+L +P +++VFSKA GP  S   Y I  DG++ Y EV+ FI  +  + + +PND +
Sbjct: 247 GWAWQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPR 306

Query: 305 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSW 360
           F IAYA+ A  TWIA+ES+ TI  K+ LA+    LGYFA N++ DD++ TL  AAK++W
Sbjct: 307 FGIAYAY-ANATWIAYESESTIPVKIELAKQNQLLGYFAWNISGDDDVNTLYNAAKTNW 355

[Close]

BLAST of Moc08g43740 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 379.8 bits (974), Expect = 2.6e-101
Identity = 197/359 (54.87%), Postives = 251/359 (69.92%), Query Frame = 0


Query: 1   MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS 60
           +T FVN GYWL++   + PA  +DATL+THLF GYA+++  +Y+V V   Q  +++ FSS
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSS 66

Query: 61  QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST 120
            VKLSNP+VKT+LSI  +  +F+ MA +   R+ FIKSSI AA  G F+GLDL WLYPS+
Sbjct: 67  TVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL 180
           QD M+NF  LI  W Q   DDA   +     LLVA+V N PY++ NI   YPA AI+Q L
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLS-LPQLLLVATVSNLPYLQSNIQ--YPADAINQKL 186

Query: 181 DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP 240
           DWVNL+SYDFYTP S    T PSSAF+N KAN LSA+FGI SW++ L +LP  KIVFGIP
Sbjct: 187 DWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIP 246

Query: 241 FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK 300
           FHGWAWKL N  QH +F+KADG   +TGEY I   G+I Y +VQ FI + QA V   D+ 
Sbjct: 247 FHGWAWKLFNSHQHTLFAKADG--AATGEY-IGPHGQIFYSDVQKFIEDNQAGVEDKDKS 306

Query: 301 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSW 360
           +++AYA+   TTWI +ES+ TI+ K+ LA++   LGYFA N+AADD   TLA AAKS+W
Sbjct: 307 YLVAYAY-YETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Moc08g43740 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-52
Identity = 130/360 (36.11%), Postives = 193/360 (53.61%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           V   YW    A++FP   +D++L+THLFC +A+++ Q+ +V V        + F+  V+ 
Sbjct: 28  VKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 65  SNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 124
            NP VKT+LSI G   +K  ++ MA N  +R+ FI SSI+ A +  F+GLDL W YPS+ 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 125 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLD 184
            +M NF  L+ EWR     +A  ++ K   LL A+V    Y      V+YP +A++ +LD
Sbjct: 148 TEMTNFGTLLREWRSAVVAEA-SSSGKPRLLLAAAV---FYSNNYYSVLYPVSAVASSLD 207

Query: 185 WVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPF 244
           WVNL++YDFY P        P++ F+   A   S   G  SW  I   LP  K V G P+
Sbjct: 208 WVNLMAYDFYGPGWSRVTGPPAALFDPSNAG-PSGDAGTRSW--IQAGLPAKKAVLGFPY 267

Query: 245 HGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRKF 304
           +G+AW+LTN   H+ ++   G         I+ DG I Y +++ FI +  A  V N    
Sbjct: 268 YGYAWRLTNANSHSYYAPTTGAA-------ISPDGSIGYGQIRKFIVDNGATTVYNST-- 327

Query: 305 VIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWSS 362
           V+     A T WI ++   +I  KV  A+    LGYF+ +V ADD    L+ AA  +W +
Sbjct: 328 VVGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAWDA 368

[Close]

BLAST of Moc08g43740 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 108/364 (29.67%), Postives = 186/364 (51.10%), Query Frame = 0


Query: 2   TNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIV----NA 61
           T  V G YW+ N    FP   +D +L+TH+F  +   +  +Y++         V      
Sbjct: 34  TTRVKGIYWIENPL--FPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTT 93

Query: 62  FSSQVKLSNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLC 121
           F++  K   P + T+LSI G      +F+ +A +   R  FI S+I+ A    F+G+D  
Sbjct: 94  FTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFD 153

Query: 122 WLYPSTQDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPAT 181
           W +P+T  +M +   L+ +WR+   D+    +R    L  A      +       +YP  
Sbjct: 154 WEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVD 213

Query: 182 AISQNLDWVNLISYDFY-TPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFD 241
           +I++NLDWVN++SYD   + ++  G  APS  F++ K+N +S   G+ SW  I G +  +
Sbjct: 214 SINKNLDWVNVMSYDLRGSGSNVTG--APSGMFDS-KSN-VSVVSGLFSW--IRGGVAPE 273

Query: 242 KIVFGIPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAE 301
           KIV G+P +G +WKL +P  H + +   GP          +DG + Y++V +F  +  A+
Sbjct: 274 KIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGP-------GVDGGMAYFQVVDFNKQMGAK 333

Query: 302 VVPNDRKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLAT 358
           VV  D++    Y+++  +TWI ++   T+S KV  A+++   GYF      D   + ++T
Sbjct: 334 VV-YDKETGSVYSYSG-STWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSDWKVST 380

[Close]

BLAST of Moc08g43740 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 154.5 bits (389), Expect = 2.4e-36
Identity = 111/364 (30.49%), Postives = 182/364 (50.00%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           V   YW +   + F    +D   +TH++  + +   +S+ + +          +   +  
Sbjct: 55  VRAAYWPA--WSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDG-IHN 114

Query: 65  SNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 124
             P V T+LSI G      +FS+MA ++  R  FI S+I  A    FNGLDL W +P  +
Sbjct: 115 IRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 125 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVV------YPATA 184
            DM N   L+ EW +    +A + +RK+  LL ++V    Y    I ++      YP  A
Sbjct: 175 KDMSNLALLLKEWYKALVVEA-NTSRKSRLLLTSAV----YFNSTISLIGNGPRSYPVRA 234

Query: 185 ISQNLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKI 244
           I + LDW + + +D+    +    T  ++A  +  +N +S  +GI SW  I   +P +K+
Sbjct: 235 IRKYLDWASPMCFDY--NGAWANETGFNAALYDPNSN-ISTKYGIGSW--IGSGVPAEKL 294

Query: 245 VFGIPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVV 304
           V G+P +G AW+L +P  H V +KA GP   T       DG + Y E+  F  +T A+VV
Sbjct: 295 VMGLPLYGRAWELKDPNDHGVGAKAVGPAVDT-------DGSMDYDEILVFNKDTGAKVV 354

Query: 305 PNDRKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAA 360
             D   V  Y+++  TTWI ++   +I++KV  ARSM   GYF   +  D + +T++  A
Sbjct: 355 -YDEVAVSFYSYSG-TTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKD-WTISKQA 395

[Close]

BLAST of Moc08g43740 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.9e-36
Identity = 106/361 (29.36%), Postives = 180/361 (49.86%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           V G YWL N    FP   +D +L+TH+F  +   +  +Y++ +   Q   +N F+   K 
Sbjct: 35  VKGIYWLENPF--FPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKT 94

Query: 65  SNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 124
            +P   T+ SI G      + + +A +      FI S+I  A    F+G+DL W +P   
Sbjct: 95  KSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNT 154

Query: 125 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVA---SVPNAPYVEPNIHVVYPATAISQ 184
            +M +   ++ +WR+   D+     R    L  A   +V  + Y EP    +YP  +I++
Sbjct: 155 KEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPR---MYPVNSINE 214

Query: 185 NLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFG 244
           NLDWVN++SY+   P S     APS  F + K+N +S   G+ SW  I   +  +K+V G
Sbjct: 215 NLDWVNVMSYELRGPRS-NKTGAPSGTF-DPKSN-VSVVSGLLSW--IHSGVVPEKLVMG 274

Query: 245 IPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPND 304
           +P +G +WKL +P  H +      P+  +G     ++G + Y++V +F  +  A+ V  D
Sbjct: 275 MPLYGKSWKLRDPNVHGI----GAPSVGSGP---GVNGLMAYFQVLDFNRQKSAK-VEYD 334

Query: 305 RKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSS 360
                 Y+++  +TWI +++  T+S KV  A+++   GYF      D   + +AT A  +
Sbjct: 335 VDTASVYSYSG-STWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKA 376

[Close]

BLAST of Moc08g43740 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.8e-35
Identity = 106/365 (29.04%), Postives = 184/365 (50.41%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           V   YW + +   F    +D   +TH+   + + +  S+++ +          F   ++ 
Sbjct: 40  VRSAYWPAGD--DFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 65  SNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 124
            +P VKT+LSI G      +FS++A  +  RE+FI S+I+ A    F+G+DL W +P TQ
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 125 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVV-------YPAT 184
            DM N   L  EW      +A+   RK   LL ++V    Y    + ++       YP  
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAK-VRRKPRLLLTSAV----YYNSTVRLIGKHGPRSYPTQ 219

Query: 185 AISQNLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDK 244
           AI++ LDW + + +D++   +    T  ++A  + K+  +S +FG+ SW K  G  P +K
Sbjct: 220 AINKYLDWASPMCFDYH--GTWDNNTDFNAALYDSKSE-ISTNFGLHSWIK-SGVRP-EK 279

Query: 245 IVFGIPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEV 304
           +V G+  +G AW+L +P  + V ++A GP   T       DG + Y E+  F  ++ A V
Sbjct: 280 LVMGLALYGRAWELKDPNVNGVGAEAVGPATDT-------DGSMNYNEILKFNKQSGANV 339

Query: 305 VPNDRKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATA 360
           V +  K  I++   A TTWI ++   +I+ KV  A+S+   GYF   +  D + ++++  
Sbjct: 340 VYD--KVAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKD-WSISKQ 382

[Close]

BLAST of Moc08g43740 vs. ExPASy TrEMBL
Match: A0A6J1CN47 (probable chitinase 10 OS=Momordica charantia OX=3673 GN=LOC111013119 PE=4 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.0e-211
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0


Query: 1   MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS 60
           MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS
Sbjct: 2   MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS 61

Query: 61  QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST 120
           QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST
Sbjct: 62  QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST 121

Query: 121 QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL 180
           QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL
Sbjct: 122 QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL 181

Query: 181 DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP 240
           DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP
Sbjct: 182 DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP 241

Query: 241 FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK 300
           FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK
Sbjct: 242 FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK 301

Query: 301 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS 360
           FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS
Sbjct: 302 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS 361

Query: 361 S 362
           S
Sbjct: 362 S 362

[Close]

BLAST of Moc08g43740 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 9.7e-110
Identity = 202/359 (56.27%), Postives = 263/359 (73.26%), Query Frame = 0


Query: 2   TNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQ 61
           T+ VNGGYWLS+   Q P   +DA L+THLFCG+AEID  SYEV VP  Q  ++N FSS 
Sbjct: 8   TSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSST 67

Query: 62  VKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 121
           V++SNPDVKT+LSIRG++++F++MA     R+ FI+SSI+ A  G FNGLDL WLYPS++
Sbjct: 68  VRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSE 127

Query: 122 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLD 181
           ++M+NF+ L+  WRQ A DDA+  +R   W+LVASV N PYVE +I  VYP   I QNLD
Sbjct: 128 EEMQNFEILLGAWRQAAVDDAKTYHR-PQWILVASVSNLPYVESSI--VYPVEEIKQNLD 187

Query: 182 WVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPF 241
           WVNLISYDFYTP S P  T PSSA +N  A  LSA+FGID+W + L +LP  +IVFGIPF
Sbjct: 188 WVNLISYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPF 247

Query: 242 HGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRKF 301
           HGWAWKL NP +H+VFSKA GP +  G+  I+ +G+I    V+ FI +  A+ V +D ++
Sbjct: 248 HGWAWKLANPDKHDVFSKALGPAK--GDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRY 307

Query: 302 VIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWS 361
            I+YA+ A TTWIA+ES+ TI  K++ A+S   LGYFA N+AADDE+ TLA AA+++WS
Sbjct: 308 SISYAY-AGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNWS 360

[Close]

BLAST of Moc08g43740 vs. ExPASy TrEMBL
Match: A0A6J1CQ93 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 6.8e-103
Identity = 200/359 (55.71%), Postives = 251/359 (69.92%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           VNGGYWLS+ A + PA  +DATLYTHLFCGY EID   +EV +P  +   V+ FS +VK+
Sbjct: 7   VNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTE---VSHFSEKVKV 66

Query: 65  SNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQDDM 124
           SNP VKT+LSIRG+ ++FS +A     R+ F+ SSIKAAV   F+GLDL WLYPS+  DM
Sbjct: 67  SNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSPTDM 126

Query: 125 ENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLDWVN 184
           +NF  LI +WRQ A DDAR+NNR    +LVASVPN PY+E N+   YP  AI QNLDWVN
Sbjct: 127 QNFKTLIVKWRQAAVDDARNNNRPER-ILVASVPNLPYIEKNVE--YPGEAIKQNLDWVN 186

Query: 185 LISYDFYTPT--SCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPFH 244
           L+ YDFYTP   S P YT PSSA  N K+N LSA FGI+SW+K    +P  KIVFGIPFH
Sbjct: 187 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKY---VPPKKIVFGIPFH 246

Query: 245 GWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFI--HETQAEVVPNDRK 304
           GWAW L N L+H+VFSKA G         +A  G++ Y EV+ FI  H + ++ +PND +
Sbjct: 247 GWAWTLANALEHDVFSKATG---------LAASGQMDYSEVKKFIDAHASVSKNIPNDPR 306

Query: 305 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSW 360
           F IAYA+ A TTWIA+ES+ TI  K+  A+    LGYFA N++ DD++ TLA AA+S+W
Sbjct: 307 FGIAYAY-AETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 346

[Close]

BLAST of Moc08g43740 vs. ExPASy TrEMBL
Match: A0A6J1CQ95 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.5e-102
Identity = 199/359 (55.43%), Postives = 252/359 (70.19%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           VNGGYWLSN A + PA  +DATLYTHLFCGYAEI+  S+EV +P  +   V+ FS +VK+
Sbjct: 7   VNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTE---VSHFSEKVKM 66

Query: 65  SNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQDDM 124
           SNP+VKT+LSI G+ ++FS +A     R+ FIKSSIKAAV   F+GLDL WLYPS+  DM
Sbjct: 67  SNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSSPTDM 126

Query: 125 ENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLDWVN 184
           +NF+ LI +WRQ   DDAR  NR A+ +LVASVPN PY+E N+   YP  AI QNLDWVN
Sbjct: 127 QNFETLIGKWRQAVVDDARTYNR-AARILVASVPNLPYIEKNVE--YPGEAIKQNLDWVN 186

Query: 185 LISYDFYTPT--SCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPFH 244
           L+ YDFYTP   S  G T PSSA  N     LSA FGI+SW+K    +P  KIVFGIPFH
Sbjct: 187 LVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLSACFGIESWTKY---VPATKIVFGIPFH 246

Query: 245 GWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFI--HETQAEVVPNDRK 304
           GWAW+L +P +++VFSKA GP  S   Y I  DG++ Y EV+ FI  +  + + +PND +
Sbjct: 247 GWAWQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPR 306

Query: 305 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSW 360
           F IAYA+ A  TWIA+ES+ TI  K+ LA+    LGYFA N++ DD++ TL  AAK++W
Sbjct: 307 FGIAYAY-ANATWIAYESESTIPVKIELAKQNQLLGYFAWNISGDDDVNTLYNAAKTNW 355

[Close]

BLAST of Moc08g43740 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.3e-101
Identity = 197/359 (54.87%), Postives = 251/359 (69.92%), Query Frame = 0


Query: 1   MTNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSS 60
           +T FVN GYWL++   + PA  +DATL+THLF GYA+++  +Y+V V   Q  +++ FSS
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSS 66

Query: 61  QVKLSNPDVKTMLSIRGEKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPST 120
            VKLSNP+VKT+LSI  +  +F+ MA +   R+ FIKSSI AA  G F+GLDL WLYPS+
Sbjct: 67  TVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 121 QDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNL 180
           QD M+NF  LI  W Q   DDA   +     LLVA+V N PY++ NI   YPA AI+Q L
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLS-LPQLLLVATVSNLPYLQSNIQ--YPADAINQKL 186

Query: 181 DWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIP 240
           DWVNL+SYDFYTP S    T PSSAF+N KAN LSA+FGI SW++ L +LP  KIVFGIP
Sbjct: 187 DWVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIP 246

Query: 241 FHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRK 300
           FHGWAWKL N  QH +F+KADG   +TGEY I   G+I Y +VQ FI + QA V   D+ 
Sbjct: 247 FHGWAWKLFNSHQHTLFAKADG--AATGEY-IGPHGQIFYSDVQKFIEDNQAGVEDKDKS 306

Query: 301 FVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSW 360
           +++AYA+   TTWI +ES+ TI+ K+ LA++   LGYFA N+AADD   TLA AAKS+W
Sbjct: 307 YLVAYAY-YETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

[Close]

BLAST of Moc08g43740 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 209.1 bits (531), Expect = 5.7e-54
Identity = 125/345 (36.23%), Postives = 191/345 (55.36%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           V   YW +   ++ P  ++D++L+THLFC +A+I+  +Y+VIV        + F+  V+ 
Sbjct: 26  VKATYWFAE--SESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 65  SNPDVKTMLSIRGE---KEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 124
            NP VKT+LSI G+      F+ MA N  +R++FI SSIK A +  F+GLDL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 125 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLD 184
            +M+NF  L+ EWR   E +AR +  K   LL A+V    Y      V++P  A++ +LD
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSG-KPRLLLTAAV---FYSYSYYSVLHPVNAVADSLD 205

Query: 185 WVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPF 244
           WVNL++YDFY   S     +P+  ++       S   G+ +W++    LP  K V G P 
Sbjct: 206 WVNLVAYDFYESGSSRVTCSPAPLYDPITTG-PSGDAGVRAWTQ--AGLPAKKAVLGFPL 265

Query: 245 HGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRKF 304
           +G+AW LT+   HN ++ + GP        I+ DG I Y +++ FI + +A +V N    
Sbjct: 266 YGYAWCLTDAKNHNYYANSSGPA-------ISPDGSIGYDQIRRFIVDNKATMVYNS-NL 325

Query: 305 VIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADD 347
           V  Y + A  TWI ++   +I  KV  A+    LGYF+ ++ ADD
Sbjct: 326 VQNYCY-AKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADD 352

[Close]

BLAST of Moc08g43740 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 208.8 bits (530), Expect = 7.5e-54
Identity = 130/360 (36.11%), Postives = 193/360 (53.61%), Query Frame = 0


Query: 5   VNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQVKL 64
           V   YW    A++FP   +D++L+THLFC +A+++ Q+ +V V        + F+  V+ 
Sbjct: 28  VKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 65  SNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPSTQ 124
            NP VKT+LSI G   +K  ++ MA N  +R+ FI SSI+ A +  F+GLDL W YPS+ 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 125 DDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQNLD 184
            +M NF  L+ EWR     +A  ++ K   LL A+V    Y      V+YP +A++ +LD
Sbjct: 148 TEMTNFGTLLREWRSAVVAEA-SSSGKPRLLLAAAV---FYSNNYYSVLYPVSAVASSLD 207

Query: 185 WVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFGIPF 244
           WVNL++YDFY P        P++ F+   A   S   G  SW  I   LP  K V G P+
Sbjct: 208 WVNLMAYDFYGPGWSRVTGPPAALFDPSNAG-PSGDAGTRSW--IQAGLPAKKAVLGFPY 267

Query: 245 HGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPNDRKF 304
           +G+AW+LTN   H+ ++   G         I+ DG I Y +++ FI +  A  V N    
Sbjct: 268 YGYAWRLTNANSHSYYAPTTGAA-------ISPDGSIGYGQIRKFIVDNGATTVYNST-- 327

Query: 305 VIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELYTLATAAKSSWSS 362
           V+     A T WI ++   +I  KV  A+    LGYF+ +V ADD    L+ AA  +W +
Sbjct: 328 VVGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAWDA 368

[Close]

BLAST of Moc08g43740 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 204.9 bits (520), Expect = 1.1e-52
Identity = 124/348 (35.63%), Postives = 188/348 (54.02%), Query Frame = 0


Query: 2   TNFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQ 61
           ++ V   YW    A  FPA  +D++L+THLFC +A+++ +SYE+ +        +AF+  
Sbjct: 4   SDVVKASYWF--PATDFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTET 63

Query: 62  VKLSNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYP 121
           V+  NP VKT+LSI G   +K+ F+ MA N  +R  FI+S+I  A +  F+GLDL W YP
Sbjct: 64  VQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYP 123

Query: 122 STQDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVYPATAISQ 181
             +++M +F  L+ EWR   E ++  +   A  L  A   ++ Y      V YP  AIS 
Sbjct: 124 RNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSNYQ----GVPYPVLAISN 183

Query: 182 NLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFG 241
           +LDW+NL++YDFY P        P+S +        S   G+  W++    LP  K V G
Sbjct: 184 SLDWINLMAYDFYGPGWSTVTGPPASLYLPTDGR--SGDSGVRDWTE--AGLPAKKAVLG 243

Query: 242 IPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPND 301
            P++GWAW L +P  +   +   GP        I+ DG+I Y ++Q +I +  A  V +D
Sbjct: 244 FPYYGWAWTLADPDVNGYDANTTGPA-------ISDDGEISYRQLQTWIVDNGATKV-HD 303

Query: 302 RKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADD 347
              V  Y + A TTWI ++S+ +I  KV  A+    LGYF+ +V  DD
Sbjct: 304 DMMVGDYCY-AGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDD 332

[Close]

BLAST of Moc08g43740 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-51
Identity = 120/347 (34.58%), Postives = 178/347 (51.30%), Query Frame = 0


Query: 3   NFVNGGYWLSNEAAQFPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNIVNAFSSQV 62
           + V   YW+      FPA  +D+T +THLFC +A++D  ++EV +       V++F+  V
Sbjct: 12  SIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTV 71

Query: 63  KLSNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGLDLCWLYPS 122
           K  N DV+T+LSI G   +K + + MA N   R+ FI SSI  A   +F GLDL W YPS
Sbjct: 72  KDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPS 131

Query: 123 TQDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIH-VVYPATAISQ 182
              +M NF  L+ EWR    +++ D   +   LL A+V    Y  P+ +   YP  AI+ 
Sbjct: 132 NDVEMANFGKLVKEWRAAVVEES-DRTNQLPLLLTAAV----YYSPDYYGEEYPVQAIAD 191

Query: 183 NLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLLSAHFGIDSWSKILGDLPFDKIVFG 242
           NLD+VN+++YDFY P   P    P++ F+       S   G+  W  +   LP  K V G
Sbjct: 192 NLDFVNIMAYDFYGPGWSPVTGPPAALFDPSNPAGRSGDSGLSKW--LEAKLPAKKAVLG 251

Query: 243 IPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHETQAEVVPND 302
             + GWAW L +   +   +  DG         I+ DG I Y +++N+I +  A    + 
Sbjct: 252 FSYCGWAWTLEDAENNGYDAATDGAA-------ISSDGSITYAKIRNYIIDNGAATFHDP 311

Query: 303 RKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAAD 346
              VI +     TTWI ++   +I  KV  A+    LGYF+ +V AD
Sbjct: 312 A--VIGFYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

[Close]

BLAST of Moc08g43740 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 125/372 (33.60%), Postives = 188/372 (50.54%), Query Frame = 0


Query: 3   NFVNGGYWLSNEAAQ--------FPADKMDATLYTHLFCGYAEIDIQSYEVIVPFGQYNI 62
           + V   YW  +  +Q         P+  +D+TL+THLFC +A++D  ++EV +       
Sbjct: 12  SIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQ 71

Query: 63  VNAFSSQVKLSNPDVKTMLSIRG---EKEIFSQMAGNRLTREVFIKSSIKAAVAGNFNGL 122
            ++F+  VK  N DV+T+LSI G   +K + + MA N   R+ FI SSI  A   +F GL
Sbjct: 72  FSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGL 131

Query: 123 DLCWLYPSTQDDMENFDYLITEWRQVAEDDARDNNRKASWLLVASVPNAPYVEPNIHVVY 182
           DL W YPS   +M NF  L+ EWR    +++ D   +   LL A+V  +P  +    V Y
Sbjct: 132 DLAWEYPSNDVEMTNFGKLLEEWRAAVVEES-DKTNQLPLLLTAAVYYSPQYD---GVEY 191

Query: 183 PATAISQNLDWVNLISYDFYTPTSCPGYTAPSSAFNNHKANLL--SAHFGIDSWSKILGD 242
           P  AI+ NLD+VN+++YDFY P   P  T P +A  +  +N    S + G+  W      
Sbjct: 192 PVKAIADNLDFVNIMAYDFYGPGWSP-VTGPPAALFHDPSNPAGRSGNSGLRKWLD-EAK 251

Query: 243 LPFDKIVFGIPFHGWAWKLTNPLQHNVFSKADGPTESTGEYEIALDGKIVYYEVQNFIHE 302
           LP  K V G P+ GWAW L +   +   +  DG         I+ DG I Y +++N+I +
Sbjct: 252 LPPKKAVLGFPYCGWAWTLEDAENNGYDAATDGAA-------ISPDGSITYAKIRNYIVD 311

Query: 303 TQAEVVPNDRKFVIAYAFNATTTWIAFESKDTISQKVNLARSMMALGYFASNVAADDELY 362
             A    +    VI +      TWI ++   +I  KV  A+    LGYF+ +V AD    
Sbjct: 312 NGAATFHDPA--VIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNC- 367

The following BLAST results are available for this feature:

BLAST of Moc08g43740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143190.1	4.1e-211	100.00	probable chitinase 10 [Momordica charantia]	[more]
XP_022159350.1	2.0e-109	56.27	chitinase-3-like protein 2 [Momordica charantia]	[more]
XP_022143253.1	1.4e-102	55.71	chitotriosidase-1-like [Momordica charantia]	[more]
XP_022143252.1	3.1e-102	55.43	acidic mammalian chitinase-like [Momordica charantia]	[more]
XP_022946077.1	2.6e-101	54.87	acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm...	[more]

BLAST of Moc08g43740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	1.1e-52	36.11	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
U5N4E3	1.3e-37	29.67	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A1B1J8Z2	2.4e-36	30.49	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072UR65	6.9e-36	29.36	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
A0A072VEP0	5.8e-35	29.04	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]

BLAST of Moc08g43740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CN47	2.0e-211	100.00	probable chitinase 10 OS=Momordica charantia OX=3673 GN=LOC111013119 PE=4 SV=1	[more]
A0A6J1DYG4	9.7e-110	56.27	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]
A0A6J1CQ93	6.8e-103	55.71	chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1	[more]
A0A6J1CQ95	1.5e-102	55.43	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 P...	[more]
A0A6J1G2P4	1.3e-101	54.87	acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE...	[more]

BLAST of Moc08g43740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19820.1	5.7e-54	36.23	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19810.1	7.5e-54	36.11	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	1.1e-52	35.63	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	1.6e-51	34.58	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	1.6e-48	33.60	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 4..345 e-value: 5.3E-56 score: 202.0
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 22..346 e-value: 3.6E-67 score: 229.1
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 4..354
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 4..354
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 7..339 e-value: 8.3E-50 score: 170.3
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 4..361 score: 43.102829
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 241..316 e-value: 3.6E-67 score: 229.1
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 3..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43740.1	Moc08g43740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0016787	hydrolase activity