Moc08g43710 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43710
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	chitotriosidase-1-like
Location	chr8: 33601792 .. 33602835 (-)
RNA-Seq Expression	Moc08g43710
Synteny	Moc08g43710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCATCCAACAATTGTGAACGGTGGTTATTGGCTGAGCAGCACGGCCGGTAAATTACCTGCTTCTAACATTGATGCTACGCTTTACACTCATCTCTTCTGCGGATATGGAGAAATCGACGCCACAAGTCATGAAGTGACGATTCCTTCAACAGAAGTGAGTCACTTCTCCGAAAAAGTGAAAGTGAGCAATCCTAGTGTAAAGACTCTGCTGTCCATTAGAGGAGACAGCAAAGTTTTCTCAGACATTGCAAAAGTACCCACAGCACGAGACACGTTCGTCAATTCCTCGATCAAGGCGGCTGTTGATTGTGGGTTTGACGGCCTGGATTTACAGTGGCTGTACCCATCTTCTCTGACTGATATGCAAAATTTTAAAATCCTGATTGTGAAGTGGCGGCAGGCGGCTGTGGACGACGCTAGAAACAACAACCGACCGGAGCGGATCCTGGTGGCTTCGGTGCCGAACCTACCATACATTGAGAAGAATGTTGAGTACCCAGGTGAAGCAATCAAACAAAACCTCGACTGGGTGAACCTTGTTTGTTACGACTTTTACACCCCAGCCGAAGGTTCGTCCCCCGATTACACTGGACCATCTTCCGCACTCTTCAACCGCAGGTCCAACTCCTTGTCCGCACACTTCGGGATCGAATCCTGGACAAAATACGTTCCTGCCAAGAAGATCGTGTTCGGAATCCCTTTCCACGGCTGGGCATGGACACTCGCCAATGCTGCCGAGCACGACGTGTTTTCGAAGGCGACTGGCCCAGCTGCGTCTGGCCAAATGGACTATTCTGAGGTGAAAAAGTTCATAGACGCGCACGCCAGTGTGAGTATAAACATCCCCAATGACCCCAGATTTGGGATTGCCTACGCCTATGCTGAAACCACCTGGATTGCGTACGAAAGTGAATCCACCATTCCTGTGAAAATTAAATTCGCAAAGCAAAACCAGCTGCTGGGTTACTTTGCTTGGAACATCTCTGGCGACGACGACGTCAACACTCTTGCTAACGCTGCCCAATCCAATTGGCAGTGA

mRNA sequence

ATGTCTCATCCAACAATTGTGAACGGTGGTTATTGGCTGAGCAGCACGGCCGGTAAATTACCTGCTTCTAACATTGATGCTACGCTTTACACTCATCTCTTCTGCGGATATGGAGAAATCGACGCCACAAGTCATGAAGTGACGATTCCTTCAACAGAAGTGAGTCACTTCTCCGAAAAAGTGAAAGTGAGCAATCCTAGTGTAAAGACTCTGCTGTCCATTAGAGGAGACAGCAAAGTTTTCTCAGACATTGCAAAAGTACCCACAGCACGAGACACGTTCGTCAATTCCTCGATCAAGGCGGCTGTTGATTGTGGGTTTGACGGCCTGGATTTACAGTGGCTGTACCCATCTTCTCTGACTGATATGCAAAATTTTAAAATCCTGATTGTGAAGTGGCGGCAGGCGGCTGTGGACGACGCTAGAAACAACAACCGACCGGAGCGGATCCTGGTGGCTTCGGTGCCGAACCTACCATACATTGAGAAGAATGTTGAGTACCCAGGTGAAGCAATCAAACAAAACCTCGACTGGGTGAACCTTGTTTGTTACGACTTTTACACCCCAGCCGAAGGTTCGTCCCCCGATTACACTGGACCATCTTCCGCACTCTTCAACCGCAGGTCCAACTCCTTGTCCGCACACTTCGGGATCGAATCCTGGACAAAATACGTTCCTGCCAAGAAGATCGTGTTCGGAATCCCTTTCCACGGCTGGGCATGGACACTCGCCAATGCTGCCGAGCACGACGTGTTTTCGAAGGCGACTGGCCCAGCTGCGTCTGGCCAAATGGACTATTCTGAGGTGAAAAAGTTCATAGACGCGCACGCCAGTGTGAGTATAAACATCCCCAATGACCCCAGATTTGGGATTGCCTACGCCTATGCTGAAACCACCTGGATTGCGTACGAAAGTGAATCCACCATTCCTGTGAAAATTAAATTCGCAAAGCAAAACCAGCTGCTGGGTTACTTTGCTTGGAACATCTCTGGCGACGACGACGTCAACACTCTTGCTAACGCTGCCCAATCCAATTGGCAGTGA

Coding sequence (CDS)

ATGTCTCATCCAACAATTGTGAACGGTGGTTATTGGCTGAGCAGCACGGCCGGTAAATTACCTGCTTCTAACATTGATGCTACGCTTTACACTCATCTCTTCTGCGGATATGGAGAAATCGACGCCACAAGTCATGAAGTGACGATTCCTTCAACAGAAGTGAGTCACTTCTCCGAAAAAGTGAAAGTGAGCAATCCTAGTGTAAAGACTCTGCTGTCCATTAGAGGAGACAGCAAAGTTTTCTCAGACATTGCAAAAGTACCCACAGCACGAGACACGTTCGTCAATTCCTCGATCAAGGCGGCTGTTGATTGTGGGTTTGACGGCCTGGATTTACAGTGGCTGTACCCATCTTCTCTGACTGATATGCAAAATTTTAAAATCCTGATTGTGAAGTGGCGGCAGGCGGCTGTGGACGACGCTAGAAACAACAACCGACCGGAGCGGATCCTGGTGGCTTCGGTGCCGAACCTACCATACATTGAGAAGAATGTTGAGTACCCAGGTGAAGCAATCAAACAAAACCTCGACTGGGTGAACCTTGTTTGTTACGACTTTTACACCCCAGCCGAAGGTTCGTCCCCCGATTACACTGGACCATCTTCCGCACTCTTCAACCGCAGGTCCAACTCCTTGTCCGCACACTTCGGGATCGAATCCTGGACAAAATACGTTCCTGCCAAGAAGATCGTGTTCGGAATCCCTTTCCACGGCTGGGCATGGACACTCGCCAATGCTGCCGAGCACGACGTGTTTTCGAAGGCGACTGGCCCAGCTGCGTCTGGCCAAATGGACTATTCTGAGGTGAAAAAGTTCATAGACGCGCACGCCAGTGTGAGTATAAACATCCCCAATGACCCCAGATTTGGGATTGCCTACGCCTATGCTGAAACCACCTGGATTGCGTACGAAAGTGAATCCACCATTCCTGTGAAAATTAAATTCGCAAAGCAAAACCAGCTGCTGGGTTACTTTGCTTGGAACATCTCTGGCGACGACGACGTCAACACTCTTGCTAACGCTGCCCAATCCAATTGGCAGTGA

Protein sequence

MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEKVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWAWTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ

Homology

[Close]

BLAST of Moc08g43710 vs. NCBI nr
Match: XP_022143018.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 694.9 bits (1792), Expect = 3.6e-196
Identity = 337/347 (97.12%), Postives = 337/347 (97.12%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDXTXHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSX 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKXLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRXSNSLSAXFGIESWTKYVPXKKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTW 300
           WTLANA EHDVFSKATG AASGQMDYSEVKKFIDAHASVS NIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANAXEHDVFSKATGXAASGQMDYSEVKKFIDAHASVSXNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

[Close]

BLAST of Moc08g43710 vs. NCBI nr
Match: XP_022143253.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 694.5 bits (1791), Expect = 4.6e-196
Identity = 337/347 (97.12%), Postives = 338/347 (97.41%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR+SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTW 300
           WTLANA EHDVFSKATG AASGQMDYSEVKKFIDAHASVS NIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

[Close]

BLAST of Moc08g43710 vs. NCBI nr
Match: AAM18075.1 (class V chitinase [Momordica charantia])

HSP 1 Score: 664.1 bits (1712), Expect = 6.7e-187
Identity = 326/347 (93.95%), Postives = 328/347 (94.52%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTW 300
           WTLANAAEHD+FSKATG +    MDYSEVK    A      NIPNDPRFGIAYAY ETTW
Sbjct: 241 WTLANAAEHDMFSKATG-SCVWPMDYSEVKSHRRAR-QCEYNIPNDPRFGIAYAYVETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           I YESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IGYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 345

[Close]

BLAST of Moc08g43710 vs. NCBI nr
Match: XP_022143252.1 (acidic mammalian chitinase-like [Momordica charantia])

HSP 1 Score: 606.7 bits (1563), Expect = 1.3e-169
Identity = 299/355 (84.23%), Postives = 315/355 (88.73%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLS+TAGKLPASNIDATLYTHLFCGY EI+ATSHEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VK+SNP+VKTLLSI GDSKVFSDIAKVPTARDTF+ SSIKAAV+ GFDGLDLQWLYPSS 
Sbjct: 61  VKMSNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNF+ LI KWRQA VDDAR  NR  RILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFETLIGKWRQAVVDDARTYNRAARILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSS   TGPSSALFNR + SLSA FGIESWTKYVPA KIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLSACFGIESWTKYVPATKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAAS---------GQMDYSEVKKFIDAHASVSINIPNDPRFGI 300
           W LA+  E+DVFSKA+GPAAS         GQM+YSEVKKFI A+A    NIPNDPRFGI
Sbjct: 241 WQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPRFGI 300

Query: 301 AYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           AYAYA  TWIAYESESTIPVKI+ AKQNQLLGYFAWNISGDDDVNTL NAA++NW
Sbjct: 301 AYAYANATWIAYESESTIPVKIELAKQNQLLGYFAWNISGDDDVNTLYNAAKTNW 355

[Close]

BLAST of Moc08g43710 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 437.2 bits (1123), Expect = 1.3e-118
Identity = 224/360 (62.22%), Postives = 272/360 (75.56%), Query Frame = 0


Query: 2   SHPT-IVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTE---VSHF 61
           S PT  VNGGYWLSST  +LP SNIDA L+THLFCG+ EID  S+EVT+P+++   ++ F
Sbjct: 5   SKPTSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQF 64

Query: 62  SEKVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYP 121
           S  V++SNP VKTLLSIRGD  VF+ +A  PT RD F+ SSI+ A    F+GLDLQWLYP
Sbjct: 65  SSTVRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYP 124

Query: 122 SSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLD 181
           SS  +MQNF+IL+  WRQAAVDDA+  +RP+ ILVASV NLPY+E ++ YP E IKQNLD
Sbjct: 125 SSEEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLD 184

Query: 182 WVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTK---YVPAKKIVFGI 241
           WVNL+ YDFYTPA  SSP  TGPSSAL NR + SLSA+FGI++W +    +P K+IVFGI
Sbjct: 185 WVNLISYDFYTPA--SSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGI 244

Query: 242 PFHGWAWTLANAAEHDVFSKATGPA-------ASGQMDYSEVKKFI-DAHASVSINIPND 301
           PFHGWAW LAN  +HDVFSKA GPA        +GQ+D S VK+FI   HA     + +D
Sbjct: 245 PFHGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQY---VKDD 304

Query: 302 PRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
            R+ I+YAYA TTWIAYESE TIP KI  AK +++LGYFAWNI+ DD+VNTLANAAQ+NW
Sbjct: 305 SRYSISYAYAGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

[Close]

BLAST of Moc08g43710 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 143/353 (40.51%), Postives = 212/353 (60.06%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPST---EVSHFSEKV 64
           T+V   YW    A + P ++ID++L+THLFC + ++++ +++VT+ S    + S F++ V
Sbjct: 26  TVVKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTV 85

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           +  NPSVKTLLSI G   D   ++ +A  PT+R +F++SSI+ A   GF GLDL W YPS
Sbjct: 86  QRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPS 145

Query: 125 SLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASV--PNLPYIEKNVEYPGEAIKQNL 184
           S T+M NF  L+ +WR A V +A ++ +P  +L A+V   N  Y   +V YP  A+  +L
Sbjct: 146 SATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSL 205

Query: 185 DWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKY-VPAKKIVFGIP 244
           DWVNL+ YDFY P        TGP +ALF+  +   S   G  SW +  +PAKK V G P
Sbjct: 206 DWVNLMAYDFYGPGWSR---VTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFP 265

Query: 245 FHGWAWTLANAAEHDVFSKATGPAAS--GQMDYSEVKKFIDAHASVSINIPNDPRFGIAY 304
           ++G+AW L NA  H  ++  TG A S  G + Y +++KFI  + + ++   N    G  Y
Sbjct: 266 YYGYAWRLTNANSHSYYAPTTGAAISPDGSIGYGQIRKFIVDNGATTVY--NSTVVG-DY 325

Query: 305 AYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
            YA T WI Y+   +I  K+++AKQ  LLGYF+W++ G DD + L+ AA   W
Sbjct: 326 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHV-GADDNSGLSRAASQAW 366

[Close]

BLAST of Moc08g43710 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.4e-49
Identity = 120/362 (33.15%), Postives = 193/362 (53.31%), Query Frame = 0


Query: 2   SHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGY-------GEIDATSHEVTIPSTEV 61
           S  T V G YW+ +     P S+ID +L+TH+F  +        +++    + T  +T +
Sbjct: 32  SSTTRVKGIYWIENPL--FPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSL 91

Query: 62  SHFSEKVKVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLD 121
           + F+   K   P + TLLSI G   +S +F+ IA  PTAR TF+NS+I+ A   GFDG+D
Sbjct: 92  TTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGID 151

Query: 122 LQWLYPSSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASV---PNLPYIEKNVEYP 181
             W +P++  +M +   L+ +WR+A  D+  + +RP  +L A+V    N     +   YP
Sbjct: 152 FDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYP 211

Query: 182 GEAIKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTK-YVPA 241
            ++I +NLDWVN++ YD      GS  + TG  S +F+ +SN +S   G+ SW +  V  
Sbjct: 212 VDSINKNLDWVNVMSYDL----RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWIRGGVAP 271

Query: 242 KKIVFGIPFHGWAWTLANAAEHDVFSK--ATGPAASGQMDYSEVKKFIDAHASVSINIPN 301
           +KIV G+P +G +W L +   H + +     GP   G M Y +V   +D +  +   +  
Sbjct: 272 EKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGPGVDGGMAYFQV---VDFNKQMGAKVVY 331

Query: 302 DPRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSN 348
           D   G  Y+Y+ +TWI Y+   T+ VK+ FA+  +L GYF W  +G D  +   + A   
Sbjct: 332 DKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFW-AAGYDTSDWKVSTASKA 382

[Close]

BLAST of Moc08g43710 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.3e-43
Identity = 111/356 (31.18%), Postives = 187/356 (52.53%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEV---SHFSEKV 64
           T V G YWL +     P + +D +L+TH+F  +   +  ++++ I S+++   + F++  
Sbjct: 33  TRVKGIYWLENPF--FPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTF 92

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           K  +P   TL SI G   +S + + IA  P A   F+NS+I  A   GFDG+DL W +P 
Sbjct: 93  KTKSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPK 152

Query: 125 SLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASV---PNLPYIEKNVEYPGEAIKQN 184
           +  +M +   ++ +WR+A  D+     RP  +L A+V    N     +   YP  +I +N
Sbjct: 153 NTKEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINEN 212

Query: 185 LDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWT-KYVPAKKIVFGI 244
           LDWVN++ Y+      G   + TG  S  F+ +SN +S   G+ SW    V  +K+V G+
Sbjct: 213 LDWVNVMSYEL----RGPRSNKTGAPSGTFDPKSN-VSVVSGLLSWIHSGVVPEKLVMGM 272

Query: 245 PFHGWAWTLANAAEHDVF--SKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIA 304
           P +G +W L +   H +   S  +GP  +G M Y +V   +D +   S  +  D      
Sbjct: 273 PLYGKSWKLRDPNVHGIGAPSVGSGPGVNGLMAYFQV---LDFNRQKSAKVEYDVDTASV 332

Query: 305 YAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVN-TLANAAQSNWQ 348
           Y+Y+ +TWI Y++  T+ +K+ FA+  +L GYF W ++G D ++  +A  A   W+
Sbjct: 333 YSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFW-VAGLDTLDWKIATQASKAWK 377

[Close]

BLAST of Moc08g43710 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 7.3e-43
Identity = 117/356 (32.87%), Postives = 180/356 (50.56%), Query Frame = 0


Query: 7   VNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEKVKVSN- 66
           V   YW          S+ID   +TH++  + +    S  + I  +      +   + N 
Sbjct: 55  VRAAYW--PAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNI 114

Query: 67  -PSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSLT 126
            P V TLLSI G   ++ +FS++A     R +F+NS+I  A    F+GLDL W +P    
Sbjct: 115 RPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDEK 174

Query: 127 DMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNV--------EYPGEAIK 186
           DM N  +L+ +W +A V +A N +R  R+L+ S     Y    +         YP  AI+
Sbjct: 175 DMSNLALLLKEWYKALVVEA-NTSRKSRLLLTSA---VYFNSTISLIGNGPRSYPVRAIR 234

Query: 187 QNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWT-KYVPAKKIVF 246
           + LDW + +C+D+     G+  + TG ++AL++  SN +S  +GI SW    VPA+K+V 
Sbjct: 235 KYLDWASPMCFDY----NGAWANETGFNAALYDPNSN-ISTKYGIGSWIGSGVPAEKLVM 294

Query: 247 GIPFHGWAWTLANAAEHDVFSKATGPA--ASGQMDYSEVKKFIDAHASVSINIPNDPRFG 306
           G+P +G AW L +  +H V +KA GPA    G MDY E+  F   +      +  D    
Sbjct: 295 GLPLYGRAWELKDPNDHGVGAKAVGPAVDTDGSMDYDEILVF---NKDTGAKVVYDEVAV 354

Query: 307 IAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
             Y+Y+ TTWI Y+   +I  K++FA+   L GYF W I  D D  T++  A + W
Sbjct: 355 SFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQASNAW 395

[Close]

BLAST of Moc08g43710 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 7.3e-43
Identity = 113/354 (31.92%), Postives = 187/354 (52.82%), Query Frame = 0


Query: 7   VNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEV---SHFSEKVKV 66
           V   YW          S ID   +TH+   + + +  S ++ I  + +    +F + ++ 
Sbjct: 40  VRSAYW--PAGDDFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 67  SNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 126
            +P VKTLLSI G   +S +FS+IA     R+ F+NS+I+ A    FDG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 127 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASV---PNLPYIEKN--VEYPGEAIKQN 186
            DM N  +L  +W  A   +A+   +P  +L ++V     +  I K+    YP +AI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 187 LDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKY-VPAKKIVFGI 246
           LDW + +C+D++    G+  + T  ++AL++ +S  +S +FG+ SW K  V  +K+V G+
Sbjct: 220 LDWASPMCFDYH----GTWDNNTDFNAALYDSKS-EISTNFGLHSWIKSGVRPEKLVMGL 279

Query: 247 PFHGWAWTLANAAEHDVFSKATGPA--ASGQMDYSEVKKFIDAHASVSINIPNDPRFGIA 306
             +G AW L +   + V ++A GPA    G M+Y+E+ KF   +     N+  D      
Sbjct: 280 ALYGRAWELKDPNVNGVGAEAVGPATDTDGSMNYNEILKF---NKQSGANVVYDKVAISF 339

Query: 307 YAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           Y+YA TTWI Y+   +I  K++FAK   L GYF W +  D D  +++  A + W
Sbjct: 340 YSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDW-SISKQASNAW 382

[Close]

BLAST of Moc08g43710 vs. ExPASy TrEMBL
Match: A0A6J1CPP6 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013006 PE=4 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.7e-196
Identity = 337/347 (97.12%), Postives = 337/347 (97.12%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDXTXHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSX 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKXLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRXSNSLSAXFGIESWTKYVPXKKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTW 300
           WTLANA EHDVFSKATG AASGQMDYSEVKKFIDAHASVS NIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANAXEHDVFSKATGXAASGQMDYSEVKKFIDAHASVSXNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

[Close]

BLAST of Moc08g43710 vs. ExPASy TrEMBL
Match: A0A6J1CQ93 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 2.2e-196
Identity = 337/347 (97.12%), Postives = 338/347 (97.41%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEID T HEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDPTKHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNR+SNSLSA FGIESWTKYVP KKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRKSNSLSARFGIESWTKYVPPKKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTW 300
           WTLANA EHDVFSKATG AASGQMDYSEVKKFIDAHASVS NIPNDPRFGIAYAYAETTW
Sbjct: 241 WTLANALEHDVFSKATGLAASGQMDYSEVKKFIDAHASVSKNIPNDPRFGIAYAYAETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 347

[Close]

BLAST of Moc08g43710 vs. ExPASy TrEMBL
Match: Q8S2V9 (Class V chitinase OS=Momordica charantia OX=3673 GN=Chi5 PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.3e-187
Identity = 326/347 (93.95%), Postives = 328/347 (94.52%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSS 
Sbjct: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNFK LIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFKTLIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAASGQMDYSEVKKFIDAHASVSINIPNDPRFGIAYAYAETTW 300
           WTLANAAEHD+FSKATG +    MDYSEVK    A      NIPNDPRFGIAYAY ETTW
Sbjct: 241 WTLANAAEHDMFSKATG-SCVWPMDYSEVKSHRRAR-QCEYNIPNDPRFGIAYAYVETTW 300

Query: 301 IAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 348
           I YESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ
Sbjct: 301 IGYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNWQ 345

[Close]

BLAST of Moc08g43710 vs. ExPASy TrEMBL
Match: A0A6J1CQ95 (acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.2e-170
Identity = 299/355 (84.23%), Postives = 315/355 (88.73%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTEVSHFSEK 60
           MSHPTIVNGGYWLS+TAGKLPASNIDATLYTHLFCGY EI+ATSHEVTIPSTEVSHFSEK
Sbjct: 1   MSHPTIVNGGYWLSNTAGKLPASNIDATLYTHLFCGYAEINATSHEVTIPSTEVSHFSEK 60

Query: 61  VKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPSSL 120
           VK+SNP+VKTLLSI GDSKVFSDIAKVPTARDTF+ SSIKAAV+ GFDGLDLQWLYPSS 
Sbjct: 61  VKMSNPNVKTLLSITGDSKVFSDIAKVPTARDTFIKSSIKAAVNSGFDGLDLQWLYPSSP 120

Query: 121 TDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180
           TDMQNF+ LI KWRQA VDDAR  NR  RILVASVPNLPYIEKNVEYPGEAIKQNLDWVN
Sbjct: 121 TDMQNFETLIGKWRQAVVDDARTYNRAARILVASVPNLPYIEKNVEYPGEAIKQNLDWVN 180

Query: 181 LVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKYVPAKKIVFGIPFHGWA 240
           LVCYDFYTPAEGSS   TGPSSALFNR + SLSA FGIESWTKYVPA KIVFGIPFHGWA
Sbjct: 181 LVCYDFYTPAEGSSAGNTGPSSALFNRNNGSLSACFGIESWTKYVPATKIVFGIPFHGWA 240

Query: 241 WTLANAAEHDVFSKATGPAAS---------GQMDYSEVKKFIDAHASVSINIPNDPRFGI 300
           W LA+  E+DVFSKA+GPAAS         GQM+YSEVKKFI A+A    NIPNDPRFGI
Sbjct: 241 WQLADPPEYDVFSKASGPAASDVGYVIGTDGQMEYSEVKKFIGANACKGKNIPNDPRFGI 300

Query: 301 AYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
           AYAYA  TWIAYESESTIPVKI+ AKQNQLLGYFAWNISGDDDVNTL NAA++NW
Sbjct: 301 AYAYANATWIAYESESTIPVKIELAKQNQLLGYFAWNISGDDDVNTLYNAAKTNW 355

[Close]

BLAST of Moc08g43710 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 6.5e-119
Identity = 224/360 (62.22%), Postives = 272/360 (75.56%), Query Frame = 0


Query: 2   SHPT-IVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPSTE---VSHF 61
           S PT  VNGGYWLSST  +LP SNIDA L+THLFCG+ EID  S+EVT+P+++   ++ F
Sbjct: 5   SKPTSCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQF 64

Query: 62  SEKVKVSNPSVKTLLSIRGDSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYP 121
           S  V++SNP VKTLLSIRGD  VF+ +A  PT RD F+ SSI+ A    F+GLDLQWLYP
Sbjct: 65  SSTVRMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYP 124

Query: 122 SSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQNLD 181
           SS  +MQNF+IL+  WRQAAVDDA+  +RP+ ILVASV NLPY+E ++ YP E IKQNLD
Sbjct: 125 SSEEEMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLD 184

Query: 182 WVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTK---YVPAKKIVFGI 241
           WVNL+ YDFYTPA  SSP  TGPSSAL NR + SLSA+FGI++W +    +P K+IVFGI
Sbjct: 185 WVNLISYDFYTPA--SSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGI 244

Query: 242 PFHGWAWTLANAAEHDVFSKATGPA-------ASGQMDYSEVKKFI-DAHASVSINIPND 301
           PFHGWAW LAN  +HDVFSKA GPA        +GQ+D S VK+FI   HA     + +D
Sbjct: 245 PFHGWAWKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQY---VKDD 304

Query: 302 PRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
            R+ I+YAYA TTWIAYESE TIP KI  AK +++LGYFAWNI+ DD+VNTLANAAQ+NW
Sbjct: 305 SRYSISYAYAGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

[Close]

BLAST of Moc08g43710 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 259.6 bits (662), Expect = 3.6e-69
Identity = 143/353 (40.51%), Postives = 212/353 (60.06%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPST---EVSHFSEKV 64
           T+V   YW    A + P ++ID++L+THLFC + ++++ +++VT+ S    + S F++ V
Sbjct: 26  TVVKASYWF--PASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTV 85

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           +  NPSVKTLLSI G   D   ++ +A  PT+R +F++SSI+ A   GF GLDL W YPS
Sbjct: 86  QRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPS 145

Query: 125 SLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASV--PNLPYIEKNVEYPGEAIKQNL 184
           S T+M NF  L+ +WR A V +A ++ +P  +L A+V   N  Y   +V YP  A+  +L
Sbjct: 146 SATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSL 205

Query: 185 DWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKY-VPAKKIVFGIP 244
           DWVNL+ YDFY P        TGP +ALF+  +   S   G  SW +  +PAKK V G P
Sbjct: 206 DWVNLMAYDFYGPGWSR---VTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFP 265

Query: 245 FHGWAWTLANAAEHDVFSKATGPAAS--GQMDYSEVKKFIDAHASVSINIPNDPRFGIAY 304
           ++G+AW L NA  H  ++  TG A S  G + Y +++KFI  + + ++   N    G  Y
Sbjct: 266 YYGYAWRLTNANSHSYYAPTTGAAISPDGSIGYGQIRKFIVDNGATTVY--NSTVVG-DY 325

Query: 305 AYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQSNW 347
            YA T WI Y+   +I  K+++AKQ  LLGYF+W++ G DD + L+ AA   W
Sbjct: 326 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHV-GADDNSGLSRAASQAW 366

[Close]

BLAST of Moc08g43710 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 250.0 bits (637), Expect = 2.8e-66
Identity = 134/342 (39.18%), Postives = 207/342 (60.53%), Query Frame = 0


Query: 2   SHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPS---TEVSHFS 61
           S  T+V   YW + +  + P + ID++L+THLFC + +I+  +++V + S    + S F+
Sbjct: 21  SAQTVVKATYWFAES--ESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFT 80

Query: 62  EKVKVSNPSVKTLLSIRGD---SKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWL 121
           + V+  NP+VKTLLSI GD   +  F+ +A  PT+R  F++SSIK A  CGF GLDL W 
Sbjct: 81  QTVRRRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWK 140

Query: 122 YPSSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGEAIKQN 181
           YPS  T+M NF  L+ +WR A   +AR++ +P  +L A+V    Y   +V +P  A+  +
Sbjct: 141 YPSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAV-FYSYSYYSVLHPVNAVADS 200

Query: 182 LDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKY-VPAKKIVFGI 241
           LDWVNLV YDFY   E  S   T   + L++  +   S   G+ +WT+  +PAKK V G 
Sbjct: 201 LDWVNLVAYDFY---ESGSSRVTCSPAPLYDPITTGPSGDAGVRAWTQAGLPAKKAVLGF 260

Query: 242 PFHGWAWTLANAAEHDVFSKATGPAAS--GQMDYSEVKKFIDAHASVSINIPNDPRFGIA 301
           P +G+AW L +A  H+ ++ ++GPA S  G + Y ++++FI  + +  +   N  +    
Sbjct: 261 PLYGYAWCLTDAKNHNYYANSSGPAISPDGSIGYDQIRRFIVDNKATMVYNSNLVQ---N 320

Query: 302 YAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDD 335
           Y YA+ TWI Y+   +I +K+K+AKQ  LLGYF+W+I  DD+
Sbjct: 321 YCYAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

[Close]

BLAST of Moc08g43710 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 240.0 bits (611), Expect = 2.9e-63
Identity = 135/346 (39.02%), Postives = 203/346 (58.67%), Query Frame = 0


Query: 1   MSHPTIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIPS---TEVSHF 60
           MS   +V   YW  +T    PA++ID++L+THLFC + +++A S+E+TI +        F
Sbjct: 1   MSESDVVKASYWFPAT--DFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAF 60

Query: 61  SEKVKVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQW 120
           +E V+  NP VKTLLSI G   D   F+ +A  P +R +F+ S+I  A   GF GLDL W
Sbjct: 61  TETVQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDW 120

Query: 121 LYPSSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKN---VEYPGEA 180
            YP +  +M +F  L+ +WR A   ++ ++     IL A+V    Y   N   V YP  A
Sbjct: 121 EYPRNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLA 180

Query: 181 IKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNSLSAHFGIESWTKY-VPAKKI 240
           I  +LDW+NL+ YDFY P   +    TGP ++L+   ++  S   G+  WT+  +PAKK 
Sbjct: 181 ISNSLDWINLMAYDFYGPGWST---VTGPPASLY-LPTDGRSGDSGVRDWTEAGLPAKKA 240

Query: 241 VFGIPFHGWAWTLANAAEHDVFSKATGPAAS--GQMDYSEVKKFIDAHASVSINIPNDPR 300
           V G P++GWAWTLA+   +   +  TGPA S  G++ Y +++ +I  + +  ++  +D  
Sbjct: 241 VLGFPYYGWAWTLADPDVNGYDANTTGPAISDDGEISYRQLQTWIVDNGATKVH--DDMM 300

Query: 301 FGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDD 335
            G  Y YA TTWI Y+SE +I  K+ +AKQ  LLGYF+W++ GDD+
Sbjct: 301 VG-DYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

[Close]

BLAST of Moc08g43710 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 141/363 (38.84%), Postives = 203/363 (55.92%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGK--------LPASNIDATLYTHLFCGYGEIDATSHEVTIP---STE 64
           +IV   YW      +         P+S ID+TL+THLFC + ++D+++HEVTI    S +
Sbjct: 12  SIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQ 71

Query: 65  VSHFSEKVKVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGL 124
            S F+E VK  N  V+TLLSI G   D  V + +A     R  F++SSI  A    F GL
Sbjct: 72  FSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGL 131

Query: 125 DLQWLYPSSLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLPYIEKNVEYPGE 184
           DL W YPS+  +M NF  L+ +WR A V+++   N+   +L A+V   P  +  VEYP +
Sbjct: 132 DLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYD-GVEYPVK 191

Query: 185 AIKQNLDWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSN--SLSAHFGIESW--TKYVP 244
           AI  NLD+VN++ YDFY P  G SP  TGP +ALF+  SN    S + G+  W     +P
Sbjct: 192 AIADNLDFVNIMAYDFYGP--GWSP-VTGPPAALFHDPSNPAGRSGNSGLRKWLDEAKLP 251

Query: 245 AKKIVFGIPFHGWAWTLANAAEHDVFSKATGPAA---SGQMDYSEVKKFIDAHASVSINI 304
            KK V G P+ GWAWTL + AE++ +  AT  AA    G + Y++++ +I  + + +   
Sbjct: 252 PKKAVLGFPYCGWAWTLED-AENNGYDAATDGAAISPDGSITYAKIRNYIVDNGAATF-- 311

Query: 305 PNDPRFGIAYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGDDDVNTLANAAQ 347
            +DP     Y Y   TWI Y+   +I  K+K+AK   LLGYF+W++  D +   L+ AA 
Sbjct: 312 -HDPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAAS 365

[Close]

BLAST of Moc08g43710 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 227.3 bits (578), Expect = 2.0e-59
Identity = 136/341 (39.88%), Postives = 197/341 (57.77%), Query Frame = 0


Query: 5   TIVNGGYWLSSTAGKLPASNIDATLYTHLFCGYGEIDATSHEVTIP---STEVSHFSEKV 64
           +IV   YW+       PA NID+T +THLFC + ++D+++HEVTI    S +VS F+  V
Sbjct: 12  SIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTV 71

Query: 65  KVSNPSVKTLLSIRG---DSKVFSDIAKVPTARDTFVNSSIKAAVDCGFDGLDLQWLYPS 124
           K  N  V+TLLSI G   D  V + +A     R  F++SSI  A    F GLDL W YPS
Sbjct: 72  KDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPS 131

Query: 125 SLTDMQNFKILIVKWRQAAVDDARNNNRPERILVASVPNLP--YIEKNVEYPGEAIKQNL 184
           +  +M NF  L+ +WR A V+++   N+   +L A+V   P  Y E   EYP +AI  NL
Sbjct: 132 NDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGE---EYPVQAIADNL 191

Query: 185 DWVNLVCYDFYTPAEGSSPDYTGPSSALFNRRSNS-LSAHFGIESWTK-YVPAKKIVFGI 244
           D+VN++ YDFY P  G SP  TGP +ALF+  + +  S   G+  W +  +PAKK V G 
Sbjct: 192 DFVNIMAYDFYGP--GWSP-VTGPPAALFDPSNPAGRSGDSGLSKWLEAKLPAKKAVLGF 251

Query: 245 PFHGWAWTLANAAEHDVFSKATGPAA---SGQMDYSEVKKFIDAHASVSINIPNDPRFGI 304
            + GWAWTL + AE++ +  AT  AA    G + Y++++ +I  + + +    +DP    
Sbjct: 252 SYCGWAWTLED-AENNGYDAATDGAAISSDGSITYAKIRNYIIDNGAATF---HDPAVIG 311

Query: 305 AYAYAETTWIAYESESTIPVKIKFAKQNQLLGYFAWNISGD 333
            Y Y  TTWI Y+   +I  K+++AK   LLGYF+W++  D
Sbjct: 312 FYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

The following BLAST results are available for this feature:

BLAST of Moc08g43710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143018.1	3.6e-196	97.12	acidic mammalian chitinase-like [Momordica charantia]	[more]
XP_022143253.1	4.6e-196	97.12	chitotriosidase-1-like [Momordica charantia]	[more]
AAM18075.1	6.7e-187	93.95	class V chitinase [Momordica charantia]	[more]
XP_022143252.1	1.3e-169	84.23	acidic mammalian chitinase-like [Momordica charantia]	[more]
XP_022159350.1	1.3e-118	62.22	chitinase-3-like protein 2 [Momordica charantia]	[more]

BLAST of Moc08g43710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	5.0e-68	40.51	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
U5N4E3	1.4e-49	33.15	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A072UR65	3.3e-43	31.18	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
A0A1B1J8Z2	7.3e-43	32.87	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072VEP0	7.3e-43	31.92	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]

BLAST of Moc08g43710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPP6	1.7e-196	97.12	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013006 P...	[more]
A0A6J1CQ93	2.2e-196	97.12	chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013164 PE=4 SV=1	[more]
Q8S2V9	3.3e-187	93.95	Class V chitinase OS=Momordica charantia OX=3673 GN=Chi5 PE=2 SV=1	[more]
A0A6J1CQ95	6.2e-170	84.23	acidic mammalian chitinase-like OS=Momordica charantia OX=3673 GN=LOC111013163 P...	[more]
A0A6J1DYG4	6.5e-119	62.22	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]

BLAST of Moc08g43710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	3.6e-69	40.51	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	2.8e-66	39.18	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	2.9e-63	39.02	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	1.5e-59	38.84	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	2.0e-59	39.88	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 6..332 e-value: 6.2E-68 score: 241.7
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 9..332 e-value: 7.5E-57 score: 193.4
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 6..347 score: 45.175934
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 236..303 e-value: 5.2E-79 score: 268.0
IPR029070	Chitinase insertion domain superfamily	SUPERFAMILY	54556	Chitinase insertion domain	coord: 237..303
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..333 e-value: 5.2E-79 score: 268.0
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 5..341
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 5..341
None	No IPR available	CDD	cd02879	GH18_plant_chitinase_class_V	coord: 4..338 e-value: 6.91895E-141 score: 399.432
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 10..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43710.1	Moc08g43710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0016787	hydrolase activity