Moc08g43680 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43680
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ribonuclease MC-like
Location	chr8: 33587786 .. 33589433 (+)
RNA-Seq Expression	Moc08g43680
Synteny	Moc08g43680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGGGAGAAACAGAGAAATTTTGATAGTGTTATTGAATGTAATAATAATGATGATGGTGTTTGGAGGAGGGGGAGCCAACTTCCAAATGTTCCAAATGGTGCAGCAGTGGCCACCGGCTTATTGCGCTACCTTCCCAAACGACTGCAACACAGCCCTGGCTCAGGCCACCACCAGGTTCACAATACATGGCTACTGGCCCAGTCTAGACAATGGAGACCTGGTAACTCACTGTACAACACATCAGCTCACCCTAGAAGAGGTAATAATTTCCACACCCAATTTTGTAACGCTATCGTTTCTAGTTTCTTTGTTGGTTGTTTCTAGTTTTTTAGAAACATAAAGTTATCTAAAAAGTTTAGAAACAACAAAACTTAAGTTTATTATTTTTTATGTTTATGTTTCTAATTTTTTCCAAAAGGTACGTAAAATCTTTTTCTCTTTTTATTTATTTACTTTTATGATACATAATAGAAATCACAAAATAAATATATAACATACAAATTTTGAAAAAGAAATAAATTAAATTTTATATAGAAACCTTTTATTTCTTTTTAATCCAAAATTTTAGAGTCCAAACATGTTTCTTTTTTCTTAAACAAAGAAAGAACAACCAACAAAATACCACCAAAGCTGTTTTTTGTTTTTTAAATAAAAAAGAGAAAGCGAGAAACAAAGATAAAAGAATTGCATCTTTTTGGATCTTCTTTAGATCTCACCCTCACTTATGAGATGATTCCAAGAATACTAGCACCGTCTTGATCTCCTAAATGTCCACTATTTTGTAATTATGATATATCCACTATTTTTCTCAATTAGAGCTTTTATCTAAAGCCTCTTTTTGTTTTTGTTTCCTCTCATCCCACTTAAATGAAATGATATCTCTCACGATAAGTAATAAACGAGAAATGAGAAACAAGAAATAAAAATATTACCATCGTAATCAGGACGGAGTATAGTACCTTTTTTTTATTATCTTCTTTCACTTGAATTTATGTTTATCTCAGATTACGAATGAAACAGCAGGACTAGATCGGGAGTGGATAACTTTCCAAAAAGGTTGGAATAACTTCCAATTTTGGAGTACACAGTGGAGCAAGCATGGAACTTGCTCTGGTCTTACCCCAGCACTATATTTCGCAGCCGCTTTGGCTCTTAAGCAGCAATATGACCTGTATGCAATTCTAGGCACGCTTGCTCCAACCACCAGTTTCACTAAAAAAGTTGACCTCCGCCAACTTCAACGTGCCATTTATAGCCGTACCGTGACGGAGCCAGCTTTTCGGTGCAGCTTCGGAGAAATTACTGAAGTGGTGTTGTGCGTCTCACCTGGGACTGCTGTAACTCTTTACGGTCCTCTTCAGAACTGCTCCGATGATGTAACTCTAAAAACTTGTTCTTTTAACCGCACCTACTTTTGGAGCCCGCAGAGGCCTCCAACACATCGCACCGCCCCTTCCTTGGTCGCTTCTTCACTGTTCGGGGTTCCGCTTCGGCTTACTTGGGCTTTTGTTGCTGTCATATTATTGTTCCTTGTCGCGGCGAGGGGGAAATTGTTGCAGATCTTCCATCATATGAGGGGGAAATTGTTGCAGATCTTTCCTAATATGAGGACTGCTAGAGCTGATCCAGTGAAGGAGGATTGA

mRNA sequence

ATGATGGGGAGAAACAGAGAAATTTTGATAGTGTTATTGAATGTAATAATAATGATGATGGTGTTTGGAGGAGGGGGAGCCAACTTCCAAATGTTCCAAATGGTGCAGCAGTGGCCACCGGCTTATTGCGCTACCTTCCCAAACGACTGCAACACAGCCCTGGCTCAGGCCACCACCAGGTTCACAATACATGGCTACTGGCCCAGTCTAGACAATGGAGACCTGGTAACTCACTGTACAACACATCAGCTCACCCTAGAAGAGATTACGAATGAAACAGCAGGACTAGATCGGGAGTGGATAACTTTCCAAAAAGGTTGGAATAACTTCCAATTTTGGAGTACACAGTGGAGCAAGCATGGAACTTGCTCTGGTCTTACCCCAGCACTATATTTCGCAGCCGCTTTGGCTCTTAAGCAGCAATATGACCTGTATGCAATTCTAGGCACGCTTGCTCCAACCACCAGTTTCACTAAAAAAGTTGACCTCCGCCAACTTCAACGTGCCATTTATAGCCGTACCGTGACGGAGCCAGCTTTTCGGTGCAGCTTCGGAGAAATTACTGAAGTGGTGTTGTGCGTCTCACCTGGGACTGCTGTAACTCTTTACGGTCCTCTTCAGAACTGCTCCGATGATGTAACTCTAAAAACTTGTTCTTTTAACCGCACCTACTTTTGGAGCCCGCAGAGGCCTCCAACACATCGCACCGCCCCTTCCTTGGTCGCTTCTTCACTGTTCGGGGTTCCGCTTCGGCTTACTTGGGCTTTTGTTGCTGTCATATTATTGTTCCTTGTCGCGGCGAGGGGGAAATTGTTGCAGATCTTCCATCATATGAGGGGGAAATTGTTGCAGATCTTTCCTAATATGAGGACTGCTAGAGCTGATCCAGTGAAGGAGGATTGA

Coding sequence (CDS)

ATGATGGGGAGAAACAGAGAAATTTTGATAGTGTTATTGAATGTAATAATAATGATGATGGTGTTTGGAGGAGGGGGAGCCAACTTCCAAATGTTCCAAATGGTGCAGCAGTGGCCACCGGCTTATTGCGCTACCTTCCCAAACGACTGCAACACAGCCCTGGCTCAGGCCACCACCAGGTTCACAATACATGGCTACTGGCCCAGTCTAGACAATGGAGACCTGGTAACTCACTGTACAACACATCAGCTCACCCTAGAAGAGATTACGAATGAAACAGCAGGACTAGATCGGGAGTGGATAACTTTCCAAAAAGGTTGGAATAACTTCCAATTTTGGAGTACACAGTGGAGCAAGCATGGAACTTGCTCTGGTCTTACCCCAGCACTATATTTCGCAGCCGCTTTGGCTCTTAAGCAGCAATATGACCTGTATGCAATTCTAGGCACGCTTGCTCCAACCACCAGTTTCACTAAAAAAGTTGACCTCCGCCAACTTCAACGTGCCATTTATAGCCGTACCGTGACGGAGCCAGCTTTTCGGTGCAGCTTCGGAGAAATTACTGAAGTGGTGTTGTGCGTCTCACCTGGGACTGCTGTAACTCTTTACGGTCCTCTTCAGAACTGCTCCGATGATGTAACTCTAAAAACTTGTTCTTTTAACCGCACCTACTTTTGGAGCCCGCAGAGGCCTCCAACACATCGCACCGCCCCTTCCTTGGTCGCTTCTTCACTGTTCGGGGTTCCGCTTCGGCTTACTTGGGCTTTTGTTGCTGTCATATTATTGTTCCTTGTCGCGGCGAGGGGGAAATTGTTGCAGATCTTCCATCATATGAGGGGGAAATTGTTGCAGATCTTTCCTAATATGAGGACTGCTAGAGCTGATCCAGTGAAGGAGGATTGA

Protein sequence

MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAFRCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSLVASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED

Homology

[Close]

BLAST of Moc08g43680 vs. NCBI nr
Match: XP_022143193.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 617.8 bits (1592), Expect = 4.8e-173
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTR 60
           MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTR
Sbjct: 1   MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTR 60

Query: 61  FTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKH 120
           FTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKH
Sbjct: 61  FTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKH 120

Query: 121 GTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF 180
           GTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF
Sbjct: 121 GTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF 180

Query: 181 RCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSL 240
           RCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSL
Sbjct: 181 RCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSL 240

Query: 241 VASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED 300
           VASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED
Sbjct: 241 VASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED 300

[Close]

BLAST of Moc08g43680 vs. NCBI nr
Match: KAE8812335.1 (aleurone ribonuclease [Hordeum vulgare])

HSP 1 Score: 102.1 bits (253), Expect = 8.8e-18
Identity = 73/235 (31.06%), Postives = 106/235 (45.11%), Query Frame = 0


Query: 9   LIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWP 68
           L+VL  ++++ +V       F  F +VQQWP ++C T    C     +  T F IHG WP
Sbjct: 3   LVVLSALLLLSLVAVSSAEEFDFFYLVQQWPGSFCDTKKGCCFPDTGKPATDFGIHGLWP 62

Query: 69  S---------LDNG-DLVTH---------CTTHQLTLEEITNETAGLDREWITFQ-KGWN 128
           +         LD   ++VT          C + +L L EI +    LD  W T   KG  
Sbjct: 63  NYAECKTRGELDGALEMVTRRRKKCWPESCNSERLKLWEIRDLVTELDANWPTLACKGGK 122

Query: 129 NFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQR 188
           +F+FW+ +W KHGTCS L    YFA AL  K +++L +IL       S T+   L  ++ 
Sbjct: 123 SFEFWTHEWEKHGTCSNLDQHGYFATALGFKARHNLTSILADAGIVPSDTETYFLSSIRD 182

Query: 189 AIYSRTVTEPAFRCSFG-----EITEVVLCVSPGTAVTLYGPLQ---NCSDDVTL 216
           AI   T       C+ G     ++ +V  C+       +  PL    NC D V L
Sbjct: 183 AIREGTGFTANLECNRGVDGETQLFQVYQCIDRDGENLIDCPLPMQGNCKDRVQL 237

[Close]

BLAST of Moc08g43680 vs. NCBI nr
Match: AAB58718.1 (aleurone ribonuclease, partial [Hordeum vulgare subsp. vulgare])

HSP 1 Score: 99.8 bits (247), Expect = 4.4e-17
Identity = 72/235 (30.64%), Postives = 105/235 (44.68%), Query Frame = 0


Query: 9   LIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWP 68
           L+VL  ++++ +V       F  F +VQQWP ++C T    C     +  T F IHG WP
Sbjct: 3   LVVLSALLLLSLVAVSSAEEFDFFYLVQQWPGSFCDTKKGCCFPDTGKPATDFGIHGLWP 62

Query: 69  S---------LDNG-DLVTH---------CTTHQLTLEEITNETAGLDREWITFQ-KGWN 128
           +         LD   ++VT          C + +L L EI +    LD  W T   KG  
Sbjct: 63  NYAECKTRGELDGALEMVTRRRKKCWPESCNSERLKLWEIRDLVTELDANWPTLACKGGK 122

Query: 129 NFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQR 188
           +F+FW+ +W KHGTCS L    Y A AL  K +++L +IL       S T+   L  ++ 
Sbjct: 123 SFEFWTHEWEKHGTCSNLDQHGYLATALGFKARHNLTSILADAGIVPSDTETYFLSSIRD 182

Query: 189 AIYSRTVTEPAFRCSFG-----EITEVVLCVSPGTAVTLYGPLQ---NCSDDVTL 216
           AI   T       C+ G     ++ +V  C+       +  PL    NC D V L
Sbjct: 183 AIREGTGFTANLECNRGVDGETQLFQVYQCIDRDGENLIDCPLPMQGNCKDRVQL 237

[Close]

BLAST of Moc08g43680 vs. NCBI nr
Match: KAF7809117.1 (extracellular ribonuclease LE-like [Senna tora])

HSP 1 Score: 99.8 bits (247), Expect = 4.4e-17
Identity = 69/225 (30.67%), Postives = 101/225 (44.89%), Query Frame = 0


Query: 8   ILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYW 67
           +L+ LL++ +   V       F  F  VQQWP +YC T  + C     +  + F+IHG W
Sbjct: 15  LLLALLDICVSKSV-------FDFFYFVQQWPGSYCDTKRSCCYPITGKPASNFSIHGLW 74

Query: 68  PSLDNGDLVTHCT-THQLTLEEITNETAGLDREWITFQ-KGWNNFQFWSTQWSKHGTCS- 127
           P+ +NG   + C   H     +I++  +     W +      NN +FW  +WSKHGTCS 
Sbjct: 75  PNYNNGSWPSDCNPKHPFNQSQISDLVSRAQTSWPSLGCPSSNNIKFWKHEWSKHGTCSE 134

Query: 128 --GLTPALYFAAALALKQQYDLYAIL--GTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF 187
             GL    YF   L LK Q DL  IL    + P  SF    ++  ++ AI   +   P  
Sbjct: 135 SVGLDQHAYFQHTLDLKDQIDLLQILQDAGIKPDGSF---YNVSSIEEAIEKGSGYFPGI 194

Query: 188 RCSF-----GEITEVVLCVSPGTAVTLYGPL---QNCSDDVTLKT 218
           RC+F      ++ E+ LCV       +  P+    NC   V   T
Sbjct: 195 RCNFDASRNSQLLEIYLCVDKSATRFIDCPILPHSNCPSQVEFPT 229

[Close]

BLAST of Moc08g43680 vs. NCBI nr
Match: SPT16861.1 (unnamed protein product [Triticum aestivum])

HSP 1 Score: 99.0 bits (245), Expect = 7.5e-17
Identity = 66/206 (32.04%), Postives = 96/206 (46.60%), Query Frame = 0


Query: 7   EILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGY 66
           ++++VL  ++++ +        F  F +VQQWP ++C T    C     +  T F+IHG 
Sbjct: 70  KLVVVLSALLLLSLAAVSSAEEFDFFYLVQQWPGSFCDTKTGCCFPDTGKPATDFSIHGM 129

Query: 67  WPS---------LDNG-DLVT---------HCTTHQLTLEEITNETAGLDREWITFQ-KG 126
           WP+         LD   ++VT          C +  L L EI +    LD  W T   KG
Sbjct: 130 WPNYAKCKTQGELDGVIEMVTKGKKKCWPEFCNSEPLQLWEIKDLVKELDANWPTLACKG 189

Query: 127 WNNFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQL 186
             + +FWS +W KHGTCS L    YFAAAL LK +++L A+L       S ++   L  +
Sbjct: 190 GKSIEFWSHEWEKHGTCSDLGQHGYFAAALGLKARHNLTAVLADTGIVPSDSETYSLSSI 249

Query: 187 QRAIYSRTVTEPAFRCSFGEITEVVL 193
             AI   T       C+ G   EV L
Sbjct: 250 NDAIKEGTGFAAKLECNRGVAGEVQL 275

[Close]

BLAST of Moc08g43680 vs. ExPASy Swiss-Prot
Match: Q40966 (Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 8.6e-16
Identity = 57/189 (30.16%), Postives = 86/189 (45.50%), Query Frame = 0


Query: 17  IMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWPSLDNGDLV 76
           +++++F      F  FQ  QQ+ PA C + P  CN    +    FT+HG WPS  NG   
Sbjct: 16  LIVLIFSASTVGFDYFQFTQQYQPAVCNSNPTPCNDPTDKL---FTVHGLWPSNRNGPDP 75

Query: 77  THCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKHGTCSGLT-----PALY 136
             C T  +  ++I N TA L+  W       ++  FW  +W KHGTC   T       L 
Sbjct: 76  EKCKTTTMNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEREWLKHGTCGYPTIKDDMHYLK 135

Query: 137 FAAALALKQQYDLYAIL--GTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAFRC-----SF 194
               + + Q+ ++ AIL   T+ P  +    VD+    R+      T+P F+C     + 
Sbjct: 136 TVIKMYITQKQNVSAILSKATIQPNGNNRSLVDIENAIRS--GNNNTKPKFKCQKNTRTT 195

[Close]

BLAST of Moc08g43680 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 5.5e-15
Identity = 53/212 (25.00%), Postives = 94/212 (44.34%), Query Frame = 0


Query: 2   MGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRF 61
           M ++++ L++ + V+  ++V      +F  F  VQQWP +YC T  + C     +    F
Sbjct: 1   MMKSQKKLLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDF 60

Query: 62  TIHGYWPSLDNGDLVTHCTTH-QLTLEEITNETAGLDREWITFQ-KGWNNFQFWSTQWSK 121
           +IHG WP+  +G    +C     L   E ++  + +++ W +      +  +FWS +W K
Sbjct: 61  SIHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLK 120

Query: 122 HGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPA 181
           HGTCS L    YF  AL  K + +L   L              +  +++AI       P 
Sbjct: 121 HGTCSALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPF 180

Query: 182 FRCSFG-----EITEVVLCVSPGTAVTLYGPL 207
             C+       ++ +V LCV    +  +  P+
Sbjct: 181 IECNVDSQGNHQLYQVYLCVDSSASKFIDCPI 212

[Close]

BLAST of Moc08g43680 vs. ExPASy Swiss-Prot
Match: O80322 (Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-14
Identity = 57/188 (30.32%), Postives = 86/188 (45.74%), Query Frame = 0


Query: 17  IMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWPSLDNGDLV 76
           +++++       +  FQ  QQ+ PA C + P  CN    +    FT+HG WPS  NG   
Sbjct: 16  LIVLILSSSTVGYDYFQFTQQYQPAVCNSNPTPCNDPTDKL---FTVHGLWPSNRNGPDP 75

Query: 77  THCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKHGTCSGLT-----PALY 136
             C T  L  ++I N TA L+  W       ++  FW  +W KHGTC   T       L 
Sbjct: 76  EKCKTTALNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEKEWIKHGTCGYPTIKDDMHYLQ 135

Query: 137 FAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRT-VTEPAFRC-----SFG 194
               + + Q+ ++ AIL   A   + T +  L  ++ AI   T  T+P F+C     +  
Sbjct: 136 TVIRMYITQKQNVSAILSKAAIQPNGTNR-PLVDIENAIRRGTNNTKPKFKCQKNTRTTT 195

[Close]

BLAST of Moc08g43680 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-13
Identity = 61/198 (30.81%), Postives = 89/198 (44.95%), Query Frame = 0


Query: 29  FQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWPSLDNGDLVTHCTT-HQLTLE 88
           F  F  VQQWPPA C+       +        FTIHG WP   +G  +T+C       + 
Sbjct: 1   FDSFWFVQQWPPAVCSF--QKSGSCPGSGLRTFTIHGLWPQ-GSGTSLTNCPQGSPFDIT 60

Query: 89  EITNETAGLDREWITFQKGWNNFQFWSTQWSKHGTCSGLT--PALYFAAALALKQQYDLY 148
           +I++  + L+  W    +  NN QFWS +W+KHGTCS  T   A YF  A+ ++  YD  
Sbjct: 61  KISHLQSQLNTLWPNVLRA-NNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYD-- 120

Query: 149 AILGTLAPTTSFTK-KVDLRQ-LQRAIYSRTVTEPAFRCSFGEITEVVLCVSPGTAVTLY 208
            I+G L P  +    +   RQ ++  + ++    P  RC     T+V   V     V  +
Sbjct: 121 -IIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQ---VVACF 180

Query: 209 GPLQNCSDDVTLKTCSFN 222
               +   D T  TC  N
Sbjct: 181 AQDGSTLIDCTRDTCGAN 188

[Close]

BLAST of Moc08g43680 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.4e-12
Identity = 50/180 (27.78%), Postives = 76/180 (42.22%), Query Frame = 0


Query: 29  FQMFQMVQQWPPAYCATFPNDCNTAL----AQATTRFTIHGYWPSLDNGDLVTHCTTHQL 88
           F  F +  QWP  YC    + C+       + A T+FTIHG WP  ++G   + C     
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 89  TLEEITNETAGLDREWITFQKG------WNNFQFWSTQWSKHGTCSGLTPAL-----YFA 148
             +EI+    GL++ W +   G           FW  +W KHGTCS  +P       YF 
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCS--SPVFHDEYNYFL 151

Query: 149 AALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAFRCSFGEITEVVLC 194
             L L  ++++  +L       S ++K  L  +  AI +     P   C    I E+ +C
Sbjct: 152 TTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAIDEIRIC 209

[Close]

BLAST of Moc08g43680 vs. ExPASy TrEMBL
Match: A0A6J1CQ41 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111013123 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.3e-173
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTR 60
           MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTR
Sbjct: 1   MMGRNREILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTR 60

Query: 61  FTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKH 120
           FTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKH
Sbjct: 61  FTIHGYWPSLDNGDLVTHCTTHQLTLEEITNETAGLDREWITFQKGWNNFQFWSTQWSKH 120

Query: 121 GTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF 180
           GTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF
Sbjct: 121 GTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAF 180

Query: 181 RCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSL 240
           RCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSL
Sbjct: 181 RCSFGEITEVVLCVSPGTAVTLYGPLQNCSDDVTLKTCSFNRTYFWSPQRPPTHRTAPSL 240

Query: 241 VASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED 300
           VASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED
Sbjct: 241 VASSLFGVPLRLTWAFVAVILLFLVAARGKLLQIFHHMRGKLLQIFPNMRTARADPVKED 300

[Close]

BLAST of Moc08g43680 vs. ExPASy TrEMBL
Match: M0Z404 (Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 4.3e-18
Identity = 73/235 (31.06%), Postives = 106/235 (45.11%), Query Frame = 0


Query: 9   LIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWP 68
           L+VL  ++++ +V       F  F +VQQWP ++C T    C     +  T F IHG WP
Sbjct: 3   LVVLSALLLLSLVAVSSAEEFDFFYLVQQWPGSFCDTKKGCCFPDTGKPATDFGIHGLWP 62

Query: 69  S---------LDNG-DLVTH---------CTTHQLTLEEITNETAGLDREWITFQ-KGWN 128
           +         LD   ++VT          C + +L L EI +    LD  W T   KG  
Sbjct: 63  NYAECKTRGELDGALEMVTRRRKKCWPESCNSERLKLWEIRDLVTELDANWPTLACKGGK 122

Query: 129 NFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQR 188
           +F+FW+ +W KHGTCS L    YFA AL  K +++L +IL       S T+   L  ++ 
Sbjct: 123 SFEFWTHEWEKHGTCSNLDQHGYFATALGFKARHNLTSILADAGIVPSDTETYFLSSIRD 182

Query: 189 AIYSRTVTEPAFRCSFG-----EITEVVLCVSPGTAVTLYGPLQ---NCSDDVTL 216
           AI   T       C+ G     ++ +V  C+       +  PL    NC D V L
Sbjct: 183 AIREGTGFTANLECNRGVDGETQLFQVYQCIDRDGENLIDCPLPMQGNCKDRVQL 237

[Close]

BLAST of Moc08g43680 vs. ExPASy TrEMBL
Match: O04392 (Aleurone ribonuclease (Fragment) OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.1e-17
Identity = 72/235 (30.64%), Postives = 105/235 (44.68%), Query Frame = 0


Query: 9   LIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYWP 68
           L+VL  ++++ +V       F  F +VQQWP ++C T    C     +  T F IHG WP
Sbjct: 3   LVVLSALLLLSLVAVSSAEEFDFFYLVQQWPGSFCDTKKGCCFPDTGKPATDFGIHGLWP 62

Query: 69  S---------LDNG-DLVTH---------CTTHQLTLEEITNETAGLDREWITFQ-KGWN 128
           +         LD   ++VT          C + +L L EI +    LD  W T   KG  
Sbjct: 63  NYAECKTRGELDGALEMVTRRRKKCWPESCNSERLKLWEIRDLVTELDANWPTLACKGGK 122

Query: 129 NFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQR 188
           +F+FW+ +W KHGTCS L    Y A AL  K +++L +IL       S T+   L  ++ 
Sbjct: 123 SFEFWTHEWEKHGTCSNLDQHGYLATALGFKARHNLTSILADAGIVPSDTETYFLSSIRD 182

Query: 189 AIYSRTVTEPAFRCSFG-----EITEVVLCVSPGTAVTLYGPLQ---NCSDDVTL 216
           AI   T       C+ G     ++ +V  C+       +  PL    NC D V L
Sbjct: 183 AIREGTGFTANLECNRGVDGETQLFQVYQCIDRDGENLIDCPLPMQGNCKDRVQL 237

[Close]

BLAST of Moc08g43680 vs. ExPASy TrEMBL
Match: A0A1D5UY15 (Genome assembly, chromosome: II OS=Triticum aestivum OX=4565 GN=CAMPLR22A2D_LOCUS1463 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.6e-17
Identity = 70/237 (29.54%), Postives = 107/237 (45.15%), Query Frame = 0


Query: 7   EILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGY 66
           ++++VL  ++++ +V       F  F +VQQWP ++C T    C     +  T F IHG 
Sbjct: 2   KLVVVLSALLLLSLVAVSSAEEFDFFYLVQQWPGSFCDTKKGCCFPDTGKPATDFGIHGL 61

Query: 67  WPS---------LDNG-DLVT---------HCTTHQLTLEEITNETAGLDREWITFQ-KG 126
           WP+         LD   ++VT         +C + +L L EI +    LD  W T   KG
Sbjct: 62  WPNYAKCKTRGELDGALEMVTKRKKKCWPENCNSERLKLWEIKDLVTELDANWPTLACKG 121

Query: 127 WNNFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQL 186
             + +FW+ +W KHGTCS L    YFA AL  K +++L  IL       S ++   L  +
Sbjct: 122 GKSIEFWTHEWEKHGTCSNLDQHGYFATALGFKARHNLTGILADAGIVPSDSETYFLSSI 181

Query: 187 QRAIYSRTVTEPAFRCSFG-----EITEVVLCVSPGTAVTLYGPLQ---NCSDDVTL 216
           + AI   T       C+ G     ++ +V  C+       +  PL    NC D V L
Sbjct: 182 RDAIKQGTGFTANLECNRGVAGETQLFQVYQCIDRAGENLIDCPLPMQGNCKDRVQL 238

[Close]

BLAST of Moc08g43680 vs. ExPASy TrEMBL
Match: M8CHP4 (Ribonuclease 1 OS=Aegilops tauschii OX=37682 GN=F775_10971 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.6e-17
Identity = 66/206 (32.04%), Postives = 96/206 (46.60%), Query Frame = 0


Query: 7   EILIVLLNVIIMMMVFGGGGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGY 66
           ++++VL  ++++ +        F  F +VQQWP ++C T    C     +  T F+IHG 
Sbjct: 2   KLVVVLSALLLLSLAAVSSAEEFDFFYLVQQWPGSFCDTKTGCCFPDTGKPATDFSIHGM 61

Query: 67  WPS---------LDNG-DLVT---------HCTTHQLTLEEITNETAGLDREWITFQ-KG 126
           WP+         LD   ++VT          C +  L L EI +    LD  W T   KG
Sbjct: 62  WPNYAKCKTQGELDGVIEMVTKGKKKCWPEFCNSEPLQLWEIKDLVKELDANWPTLACKG 121

Query: 127 WNNFQFWSTQWSKHGTCSGLTPALYFAAALALKQQYDLYAILGTLAPTTSFTKKVDLRQL 186
             + +FWS +W KHGTCS L    YFAAAL LK +++L A+L       S ++   L  +
Sbjct: 122 GKSIEFWSHEWEKHGTCSDLGQHGYFAAALGLKARHNLTAVLADTGIVPSDSETYSLSSI 181

Query: 187 QRAIYSRTVTEPAFRCSFGEITEVVL 193
             AI   T       C+ G   EV L
Sbjct: 182 NDAIKEGTGFAAKLECNRGVAGEVQL 207

[Close]

BLAST of Moc08g43680 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 82.0 bits (201), Expect = 8.8e-16
Identity = 59/198 (29.80%), Postives = 89/198 (44.95%), Query Frame = 0


Query: 13  LNVIIMMMVFGG-----GGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIHGYW 72
           +NV++ ++VF G        +F  F  V QWP AYC T    C     +    F IHG W
Sbjct: 7   VNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLW 66

Query: 73  PSLDNGDLVTHC-TTHQLTLEEITNETAGLDREWITFQKGWN-NFQFWSTQWSKHGTCSG 132
           P+ + G   ++C    Q    +I++  + L + W T     N  F FW  +W KHGTCS 
Sbjct: 67  PNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSE 126

Query: 133 --LTPALYFAAALALKQQYDLYAIL--GTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAFR 192
             +    YF  AL LKQ+ +L  IL    + P   F    +L ++  AI       P   
Sbjct: 127 SVMDQHEYFENALKLKQKANLLQILKNSGINPDDGF---YNLDKITNAIKDGIGFTPGIE 186

Query: 193 CS-----FGEITEVVLCV 195
           C+       ++ ++ +CV
Sbjct: 187 CNKDPERNAQLHQIYICV 201

[Close]

BLAST of Moc08g43680 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-13
Identity = 50/180 (27.78%), Postives = 76/180 (42.22%), Query Frame = 0


Query: 29  FQMFQMVQQWPPAYCATFPNDCNTAL----AQATTRFTIHGYWPSLDNGDLVTHCTTHQL 88
           F  F +  QWP  YC    + C+       + A T+FTIHG WP  ++G   + C     
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 89  TLEEITNETAGLDREWITFQKG------WNNFQFWSTQWSKHGTCSGLTPAL-----YFA 148
             +EI+    GL++ W +   G           FW  +W KHGTCS  +P       YF 
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCS--SPVFHDEYNYFL 151

Query: 149 AALALKQQYDLYAILGTLAPTTSFTKKVDLRQLQRAIYSRTVTEPAFRCSFGEITEVVLC 194
             L L  ++++  +L       S ++K  L  +  AI +     P   C    I E+ +C
Sbjct: 152 TTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDAIDEIRIC 209

[Close]

BLAST of Moc08g43680 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 62.4 bits (150), Expect = 7.2e-10
Identity = 51/188 (27.13%), Postives = 77/188 (40.96%), Query Frame = 0


Query: 6   REILIVLLNVIIMMMVFGG-GGANFQMFQMVQQWPPAYCATFPNDCNTALAQATTRFTIH 65
           R I+IV L ++  ++V       +F  F  V  WP A C +    C        + F IH
Sbjct: 2   RGIIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIH 61

Query: 66  GYWPSLDNGDLVTHC-TTHQLTLEEITNETAGLDREWITFQKGW----NNFQFWSTQWSK 125
           G WP  +NG     C  T+   + +I++    ++++W T    W    N    W  +W+K
Sbjct: 62  GLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKW-TEWGVWACPSNETNLWEHEWNK 121

Query: 126 HGTC--SGLTPALYFAAALALKQQYDLYAIL--GTLAPTTSFTKKVDLRQLQRAIYSRTV 184
           HGTC  S      YF   L  K +  L  IL    + P   F     L +++ AI     
Sbjct: 122 HGTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGF---YSLDEIKNAIKCAIG 181

[Close]

BLAST of Moc08g43680 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 49.3 bits (116), Expect = 6.3e-06
Identity = 25/82 (30.49%), Postives = 38/82 (46.34%), Query Frame = 0


Query: 29  FQMFQMVQQWPPAYCATFPNDCNTAL----AQATTRFTIHGYWPSLDNGDLVTHCTTHQL 88
           F  F +  QWP  YC    + C+       + A T+FTIHG WP  ++G   + C     
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 89  TLEEITNETAGLDREWITFQKG 107
             +EI+    GL++ W +   G
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCG 113

The following BLAST results are available for this feature:

BLAST of Moc08g43680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143193.1	4.8e-173	100.00	ribonuclease MC-like [Momordica charantia]	[more]
KAE8812335.1	8.8e-18	31.06	aleurone ribonuclease [Hordeum vulgare]	[more]
AAB58718.1	4.4e-17	30.64	aleurone ribonuclease, partial [Hordeum vulgare subsp. vulgare]	[more]
KAF7809117.1	4.4e-17	30.67	extracellular ribonuclease LE-like [Senna tora]	[more]
SPT16861.1	7.5e-17	32.04	unnamed protein product [Triticum aestivum]	[more]

BLAST of Moc08g43680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q40966	8.6e-16	30.16	Ribonuclease S-4 OS=Pyrus pyrifolia OX=3767 PE=1 SV=2	[more]
P80196	5.5e-15	25.00	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
O80322	1.2e-14	30.32	Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
P23540	1.8e-13	30.81	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42814	3.4e-12	27.78	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]

BLAST of Moc08g43680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQ41	2.3e-173	100.00	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111013123 PE=3 SV=1	[more]
M0Z404	4.3e-18	31.06	Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1	[more]
O04392	2.1e-17	30.64	Aleurone ribonuclease (Fragment) OS=Hordeum vulgare OX=4513 PE=2 SV=1	[more]
A0A1D5UY15	3.6e-17	29.54	Genome assembly, chromosome: II OS=Triticum aestivum OX=4565 GN=CAMPLR22A2D_LOCU...	[more]
M8CHP4	3.6e-17	32.04	Ribonuclease 1 OS=Aegilops tauschii OX=37682 GN=F775_10971 PE=3 SV=1	[more]

BLAST of Moc08g43680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	8.8e-16	29.80	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.4e-13	27.78	ribonuclease 2	[more]
AT1G14210.1	7.2e-10	27.13	Ribonuclease T2 family protein	[more]
AT2G39780.2	6.3e-06	30.49	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 29..196 e-value: 2.9E-32 score: 112.3
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 11..192
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 26..227 e-value: 9.4E-41 score: 142.1
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 28..199
None	No IPR available	PANTHER	PTHR11240:SF18	OS07G0630400 PROTEIN	coord: 11..192
None	No IPR available	CDD	cd00374	RNase_T2	coord: 29..225 e-value: 4.10605E-29 score: 108.312
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 112..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43680.1	Moc08g43680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding