Moc08g43650 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43650
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	chitotriosidase-1-like
Location	chr8: 33569279 .. 33571497 (-)
RNA-Seq Expression	Moc08g43650
Synteny	Moc08g43650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCCAAGTTTTGTCCGTGCTGCTTACTGGTTTGCCAGCACCAATGCCGAGCTTCCTTCTGCCAATATCGATGCTAACTTTTTCACCCATCTGTTTTGCGGGTATGCTATTGTAAACCCCCAAACTTACGAGGTCACCATTGATTCTTCAGAAGCTACGATAATCAATTATTTTACTTTGACTGTCAGTCTAAGCAATCCGACAGTCAAAACTCTGCTATCCATCAAAGGAGATGGAAGCGTACTCGCTAAAATGGCTAGCGAACCCAAAAATCGTGAAACCTTTATTAAGTCCTCGATTAAGGAAGCAAAAGAGGGAACGTTTGATGGCTTGGATCTACAGTGGCTGTACCCATCTTCTCAAGAAGAAATGGAAAATTTCGAAAGCCTTCTTATTGAGTGGCATAGCGCTGTTGTTGAGGATGCTAGGAATTACCATAGACAACAGTTGATCCTTGTTGCTGCAGTCTCCAATTTGCCCATTGTACACCACAATATTCAGTATCCAATTGATACAATAATACAAACCTTGGATTGGGTCAACCTTTTTTCTTATGATTTTTACACTCCGACTTCTTCAGTGAAGTGTACTGGACCATCCTCAGCACTCTACAATCCAAAGACAGACTCCTTGTCTGTAAATTCCGGTATGAATTAATTCAAACGCTAAATATGTGTTTTAGTCTCTAATATATATATGTGTGTGTTTTTTGTTTGTATTGTTTACGATCTTTATGTTAACAAATATCAAAACTTAATTGTGTGATTTTTTTTGTTAGTCAATATTAAGATTGGCCAAAGCGAACATACCTCAACGGTAATTAACTAGAGGTTGTGAGTTCGAATCTCCTCCCTAATATGTTGTACTAAAAAAAATGGTCAATATTAAGATTGACAGGGATCATAAACTTGGTGTTTCAATAATTAGTGTGTGCTTTGGCATATGCTTGGTGGAATATATGGAAATCAAAACCAAGTGGCAACCTTTATTAGATCTCATTATTTTAACAGATCTGATTTTGGATAGGAGAGCTAGTTTGAGCTTTTGGGCAGGGGCACAATATTAATATTATTTATAGGATTGGTATATCATAAACATTTGTTAGGTTTCTTATCTTTTCAAACTGCACACCCACCGTTCCTTAATTCCATTTCATTTGCTCTTTTCATGCATTCTTAACACCGGTTATTTGTGTCAATCTAAGTCTATCTCAACTAGTCAAGATATGTAACTTCGACCAAAAAGTCGTGTGTTCGAATTCCCACCCCACATGTTGTTGTACTAAAAAACCTCTGATTACTCGTGTTGAATTTATGGAACAGGTATCGAGGCATGGATCAAATGTTATCCTAATTTGCCTTCCCAACAGATTGTATTTGGAATTCCTTTCCACGGTTGGGCATGGAAACTTGCCGATCGTCTCCAGCACGACGTGTTTTCGGAGGCGGATGGAGCAGCAATAGGACACGATATTTCTTCAGATGGCCAAATCTACTACTCCAATATTAAGAAGTTTATAGAGAGTAATAAGGCTAAGAATCTAAAAAATGACGGCAAATATGTAATTGGGTACACATATTTTGATACAACTTGGATTGCCTACGAAAATGAAGTAACCATACCAGAGAAGCTTAGATTGGCAAAGTCCCTCCACGTGCTTGGCTACTTTGCTTCCAACATTGCAGCCGATGATGATGTTAACACTCTTGCTAACGCAGCTAAAGCCAATTCGTGGCTGAGGCTGCCCAAATAAGTAGGAGATTTTTATGTTTTGTAGTTTCTATTGTCTTTTGTGTTGTGTGTGTAAGATTTGAGGTCTTGATGTATCCAATAAAATGAAATGCGGGTTGTCTTTCCGTGAACTACATGTACAGGTTGATGGTTTTGTCCATCCATCGGACCCTCTTCCCCTTGTTTTTCAACCACCCAGCTCGATCCTCCCCTCTGTTGTCGTGGCGCTAGGTAAGCAAGTATGAAGGAGAGGCTAGAAAAGGCGAGGCAAGAGGCTCTCCGTTTCTAGGAATATTGGAACCTCTAAAGGTAGATGTTCGGAAGATGAGAATGCAGTTGCTTCTCAAAGAACGACTAGGGATGAAAGGCCAACCAAGATCCAAAGGGCAAAACTTCTACGACAGTTTCCCCCTTCGTCGCTTTTGGCTAAGCATCTTTCGGCGGAGGCGACTAGTCGCTTCTCTGGCAAAGTTGCGAGTTTGTGA

mRNA sequence

ATGGACCCAAGTTTTGTCCGTGCTGCTTACTGGTTTGCCAGCACCAATGCCGAGCTTCCTTCTGCCAATATCGATGCTAACTTTTTCACCCATCTGTTTTGCGGGTATGCTATTGTAAACCCCCAAACTTACGAGGTCACCATTGATTCTTCAGAAGCTACGATAATCAATTATTTTACTTTGACTGTCAGTCTAAGCAATCCGACAGTCAAAACTCTGCTATCCATCAAAGGAGATGGAAGCGTACTCGCTAAAATGGCTAGCGAACCCAAAAATCGTGAAACCTTTATTAAGTCCTCGATTAAGGAAGCAAAAGAGGGAACGTTTGATGGCTTGGATCTACAGTGGCTGTACCCATCTTCTCAAGAAGAAATGGAAAATTTCGAAAGCCTTCTTATTGAGTGGCATAGCGCTGTTGTTGAGGATGCTAGGAATTACCATAGACAACAGTTGATCCTTGTTGCTGCAGTCTCCAATTTGCCCATTGTACACCACAATATTCAGTATCCAATTGATACAATAATACAAACCTTGGATTGGGTCAACCTTTTTTCTTATGATTTTTACACTCCGACTTCTTCAGTGAAGTGTACTGGACCATCCTCAGCACTCTACAATCCAAAGACAGACTCCTTGTCTGTAAATTCCGGTATCGAGGCATGGATCAAATGTTATCCTAATTTGCCTTCCCAACAGATTGTATTTGGAATTCCTTTCCACGGTTGGGCATGGAAACTTGCCGATCGTCTCCAGCACGACGTGTTTTCGGAGGCGGATGGAGCAGCAATAGGACACGATATTTCTTCAGATGGCCAAATCTACTACTCCAATATTAAGAAGTTTATAGAGAGTAATAAGGCTAAGAATCTAAAAAATGACGGCAAATATGTAATTGGGTACACATATTTTGATACAACTTGGATTGCCTACGAAAATGAAGTAACCATACCAGAGAAGCTTAGATTGGCAAAGTCCCTCCACGTGCTTGGCTACTTTGCTTCCAACATTGCAGCCGATGATGATGTTGATGGTTTTGTCCATCCATCGGACCCTCTTCCCCTTGTTTTTCAACCACCCAGCTCGATCCTCCCCTCTGTTGTCGTGGCGCTAGGTAGATGTTCGGAAGATGAGAATGCAGTTGCTTCTCAAAGAACGACTAGGGATGAAAGGCCAACCAAGATCCAAAGGGCAAAACTTCTACGACAGTTTCCCCCTTCGTCGCTTTTGGCTAAGCATCTTTCGGCGGAGGCGACTAGTCGCTTCTCTGGCAAAGTTGCGAGTTTGTGA

Coding sequence (CDS)

ATGGACCCAAGTTTTGTCCGTGCTGCTTACTGGTTTGCCAGCACCAATGCCGAGCTTCCTTCTGCCAATATCGATGCTAACTTTTTCACCCATCTGTTTTGCGGGTATGCTATTGTAAACCCCCAAACTTACGAGGTCACCATTGATTCTTCAGAAGCTACGATAATCAATTATTTTACTTTGACTGTCAGTCTAAGCAATCCGACAGTCAAAACTCTGCTATCCATCAAAGGAGATGGAAGCGTACTCGCTAAAATGGCTAGCGAACCCAAAAATCGTGAAACCTTTATTAAGTCCTCGATTAAGGAAGCAAAAGAGGGAACGTTTGATGGCTTGGATCTACAGTGGCTGTACCCATCTTCTCAAGAAGAAATGGAAAATTTCGAAAGCCTTCTTATTGAGTGGCATAGCGCTGTTGTTGAGGATGCTAGGAATTACCATAGACAACAGTTGATCCTTGTTGCTGCAGTCTCCAATTTGCCCATTGTACACCACAATATTCAGTATCCAATTGATACAATAATACAAACCTTGGATTGGGTCAACCTTTTTTCTTATGATTTTTACACTCCGACTTCTTCAGTGAAGTGTACTGGACCATCCTCAGCACTCTACAATCCAAAGACAGACTCCTTGTCTGTAAATTCCGGTATCGAGGCATGGATCAAATGTTATCCTAATTTGCCTTCCCAACAGATTGTATTTGGAATTCCTTTCCACGGTTGGGCATGGAAACTTGCCGATCGTCTCCAGCACGACGTGTTTTCGGAGGCGGATGGAGCAGCAATAGGACACGATATTTCTTCAGATGGCCAAATCTACTACTCCAATATTAAGAAGTTTATAGAGAGTAATAAGGCTAAGAATCTAAAAAATGACGGCAAATATGTAATTGGGTACACATATTTTGATACAACTTGGATTGCCTACGAAAATGAAGTAACCATACCAGAGAAGCTTAGATTGGCAAAGTCCCTCCACGTGCTTGGCTACTTTGCTTCCAACATTGCAGCCGATGATGATGTTGATGGTTTTGTCCATCCATCGGACCCTCTTCCCCTTGTTTTTCAACCACCCAGCTCGATCCTCCCCTCTGTTGTCGTGGCGCTAGGTAGATGTTCGGAAGATGAGAATGCAGTTGCTTCTCAAAGAACGACTAGGGATGAAAGGCCAACCAAGATCCAAAGGGCAAAACTTCTACGACAGTTTCCCCCTTCGTCGCTTTTGGCTAAGCATCTTTCGGCGGAGGCGACTAGTCGCTTCTCTGGCAAAGTTGCGAGTTTGTGA

Protein sequence

MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVDGFVHPSDPLPLVFQPPSSILPSVVVALGRCSEDENAVASQRTTRDERPTKIQRAKLLRQFPPSSLLAKHLSAEATSRFSGKVASL

Homology

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BLAST of Moc08g43650 vs. NCBI nr
Match: XP_022143191.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 697.2 bits (1798), Expect = 8.8e-197
Identity = 342/343 (99.71%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60
           MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT
Sbjct: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60

Query: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120
           LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS
Sbjct: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120

Query: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180
           SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW
Sbjct: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180

Query: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240
           VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH
Sbjct: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240

Query: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGY 300
           GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGY
Sbjct: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGY 300

Query: 301 TYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVD 344
           TYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDV+
Sbjct: 301 TYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVN 343

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BLAST of Moc08g43650 vs. NCBI nr
Match: XP_022143178.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 443.0 bits (1138), Expect = 3.0e-120
Identity = 215/294 (73.13%), Postives = 248/294 (84.35%), Query Frame = 0


Query: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60
           M P  VR  YWF ST AELPSANIDA FF+HLFCGY IV+P+TY+VTIDSSE  +IN F+
Sbjct: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60

Query: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120
            TV LSNP VKTLLSI+GDG VL KM SE  NRE FI SSI+ A EG F+GLDL+WLYPS
Sbjct: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120

Query: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180
           SQ+EME FE++LIEWHSA+VEDA+NYHRQQLILVAAVSNLPIV HNIQYPIDTIIQTLDW
Sbjct: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180

Query: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240
           VNL SYDFYTP SS   TGPSSALYN KT+ +S++SGI++WI  +PNLPS++IVFGIPFH
Sbjct: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240

Query: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDG 295
           GWAWKLAD  +HD+FS+A+G A+G  ISSDG+I YSNIKKFI+ N+A+N K  G
Sbjct: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMG 294

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BLAST of Moc08g43650 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 421.8 bits (1083), Expect = 7.2e-114
Identity = 211/341 (61.88%), Postives = 258/341 (75.66%), Query Frame = 0


Query: 4   SFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTV 63
           S V   YW +STN +LP +NIDA  FTHLFCG+A ++  +YEVT+ +S+AT+IN F+ TV
Sbjct: 9   SCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTV 68

Query: 64  SLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQE 123
            +SNP VKTLLSI+GD  V AKMASEP  R+ FI+SSI+ A +G F+GLDLQWLYPSS+E
Sbjct: 69  RMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEE 128

Query: 124 EMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNL 183
           EM+NFE LL  W  A V+DA+ YHR Q ILVA+VSNLP V  +I YP++ I Q LDWVNL
Sbjct: 129 EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNL 188

Query: 184 FSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWA 243
            SYDFYTP SS   TGPSSAL+N    SLS N GI+AWI+   NLP +QIVFGIPFHGWA
Sbjct: 189 ISYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWA 248

Query: 244 WKLADRLQHDVFSEADGAAIGHD-ISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTY 303
           WKLA+  +HDVFS+A G A G   IS +GQI  SN+K+FI+ N A+ +K+D +Y I Y Y
Sbjct: 249 WKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAY 308

Query: 304 FDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVD 344
             TTWIAYE+E TIP+K+  AKS  VLGYFA NIAADD+V+
Sbjct: 309 AGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVN 349

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BLAST of Moc08g43650 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.8 bits (1070), Expect = 2.3e-112
Identity = 204/336 (60.71%), Postives = 249/336 (74.11%), Query Frame = 0


Query: 5   FVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVS 64
           FV A YW AST+  LP+ANIDA  FTHLF GYA +N  TY+VTID S+  ++N F+ TV 
Sbjct: 16  FVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVR 75

Query: 65  LSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEE 124
           LSNP VKTLLSI  D +V A MA++P NR+ FIKSSI  A+ G FDGLDLQWLYPSSQEE
Sbjct: 76  LSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEE 135

Query: 125 MENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLF 184
           M NF++L+  WH AVV+DA +    QL+LVA VSNLP +  NIQYP D I Q LDWVNL 
Sbjct: 136 MGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLL 195

Query: 185 SYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAW 244
           SYDFYTP S+   TGPSSA +NPK ++LS N GI +W +   NLP ++IVFGIPFHGWAW
Sbjct: 196 SYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAW 255

Query: 245 KLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFD 304
           KL +  QH +F++ADGAA G  I   GQI+YS+++KFIE N+A     D  Y++ YTYF+
Sbjct: 256 KLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYFE 315

Query: 305 TTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADD 341
           TTWI YE++ TI +K+ LAK+  VLGYFA NIAADD
Sbjct: 316 TTWITYESDYTIADKIALAKADKVLGYFAFNIAADD 351

[Close]

BLAST of Moc08g43650 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.5 bits (1064), Expect = 1.1e-111
Identity = 203/335 (60.60%), Postives = 248/335 (74.03%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65
           V A YW AST+  LP+ANIDA  FTHLF GYA +N  TY+VTID S+  ++N F+ TV L
Sbjct: 2   VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 61

Query: 66  SNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEEM 125
           SNP VKTLLSI  D +V A MA++P NR+ FIKSSI  A+ G FDGLDLQWLYPSSQEEM
Sbjct: 62  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 121

Query: 126 ENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLFS 185
            NF++L+  WH AVV+DA +    QL+LVA VSNLP +  NIQYP D I Q LDWVNL S
Sbjct: 122 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 181

Query: 186 YDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAWK 245
           YDFYTP S+   TGPSSA +NPK ++LS N GI +W +   NLP ++IVFGIPFHGWAWK
Sbjct: 182 YDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 241

Query: 246 LADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDT 305
           L +  QH +F++ADGAA G  I   GQI+YS+++KFIE N+A     D  Y++ YTYF+T
Sbjct: 242 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYFET 301

Query: 306 TWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADD 341
           TWI YE++ TI +K+ LAK+  VLGYFA NIAADD
Sbjct: 302 TWITYESDYTIADKIALAKADKVLGYFAFNIAADD 336

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BLAST of Moc08g43650 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 5.6e-69
Identity = 150/347 (43.23%), Postives = 215/347 (61.96%), Query Frame = 0


Query: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60
           M  + V+A+YWF +  +E P  +ID++ FTHLFC +A +N QT +VT+ S+     + FT
Sbjct: 23  MAQTVVKASYWFPA--SEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFT 82

Query: 61  LTVSLSNPTVKTLLSIKG---DGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWL 120
            TV   NP+VKTLLSI G   D +  A MAS P +R++FI SSI+ A+   F GLDL W 
Sbjct: 83  QTVQRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWE 142

Query: 121 YPSSQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV--SNLPIVHHNIQYPIDTII 180
           YPSS  EM NF +LL EW SAVV +A +  + +L+L AAV  SN    ++++ YP+  + 
Sbjct: 143 YPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSN---NYYSVLYPVSAVA 202

Query: 181 QTLDWVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVF 240
            +LDWVNL +YDFY P  S + TGP +AL++P     S ++G  +WI+    LP+++ V 
Sbjct: 203 SSLDWVNLMAYDFYGPGWS-RVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVL 262

Query: 241 GIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGK 300
           G P++G+AW+L +   H  ++   GAA    IS DG I Y  I+KFI  N A  + N   
Sbjct: 263 GFPYYGYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVYN--S 322

Query: 301 YVIG-YTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDD 342
            V+G Y Y  T WI Y++  +I  K+R AK   +LGYF+ ++ ADD+
Sbjct: 323 TVVGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN 355

[Close]

BLAST of Moc08g43650 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.6e-44
Identity = 112/333 (33.63%), Postives = 177/333 (53.15%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65
           V+  YW    N   P   +D + FTH+F  +   N  TY++ I SS+   +N FT T   
Sbjct: 35  VKGIYWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKT 94

Query: 66  SNPTVKTLLSIKGDG---SVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQ 125
            +P   TL SI G G   S+LA +AS+P     FI S+I  A+   FDG+DL W +P + 
Sbjct: 95  KSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNT 154

Query: 126 EEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV---SNLPIVHHNIQYPIDTIIQTLD 185
           +EM +   +L +W  A+ ++     R  L+L AAV    N  I      YP+++I + LD
Sbjct: 155 KEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLD 214

Query: 186 WVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPF 245
           WVN+ SY+   P S+   TG  S  ++PK+ ++SV SG+ +WI  +  +  +++V G+P 
Sbjct: 215 WVNVMSYELRGPRSNK--TGAPSGTFDPKS-NVSVVSGLLSWI--HSGVVPEKLVMGMPL 274

Query: 246 HGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIG 305
           +G +WKL D   H + + + G+  G     +G + Y  +  F     AK ++ D      
Sbjct: 275 YGKSWKLRDPNVHGIGAPSVGSGPG----VNGLMAYFQVLDFNRQKSAK-VEYDVDTASV 334

Query: 306 YTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYF 333
           Y+Y  +TWI Y+N  T+  K+  A++L + GYF
Sbjct: 335 YSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYF 355

[Close]

BLAST of Moc08g43650 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 9.0e-43
Identity = 114/343 (33.24%), Postives = 194/343 (56.56%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65
           VRAAYW A   ++  +++ID N+FTH++  +    P+++ + I  S       +   +  
Sbjct: 55  VRAAYWPAW--SDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYD-GIHN 114

Query: 66  SNPTVKTLLSIKGDG---SVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQ 125
             P V TLLSI G G   ++ ++MAS  +NR +FI S+I  A++  F+GLDL W +P  +
Sbjct: 115 IRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 126 EEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV---SNLPIVHHNIQ-YPIDTIIQTL 185
           ++M N   LL EW+ A+V +A    + +L+L +AV   S + ++ +  + YP+  I + L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 186 DWVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIP 245
           DW +   +D+    ++   TG ++ALY+P + ++S   GI +WI     +P++++V G+P
Sbjct: 235 DWASPMCFDYNGAWANE--TGFNAALYDPNS-NISTKYGIGSWIG--SGVPAEKLVMGLP 294

Query: 246 FHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVI 305
            +G AW+L D   H V ++A G A    + +DG + Y  I  F +   AK +  D   V 
Sbjct: 295 LYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGAK-VVYDEVAVS 354

Query: 306 GYTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDD 342
            Y+Y  TTWI Y++  +I +K++ A+S+ + GYF   I  D D
Sbjct: 355 FYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKD 384

[Close]

BLAST of Moc08g43650 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 2.0e-42
Identity = 116/344 (33.72%), Postives = 191/344 (55.52%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65
           VR+AYW A    +   + ID N+FTH+   +    P ++++ I  S       F   +  
Sbjct: 40  VRSAYWPAGD--DFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 66  SNPTVKTLLSIKGDGS---VLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQ 125
            +P VKTLLSI G GS   + +++AS  +NRE FI S+I+ A++  FDG+DL W +P +Q
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 126 EEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV---SNLPIV--HHNIQYPIDTIIQT 185
           ++M N   L  EW++A+  +A+   + +L+L +AV   S + ++  H    YP   I + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 186 LDWVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGI 245
           LDW +   +D++    +   T  ++ALY+ K++ +S N G+ +WIK    +  +++V G+
Sbjct: 220 LDWASPMCFDYHGTWDN--NTDFNAALYDSKSE-ISTNFGLHSWIK--SGVRPEKLVMGL 279

Query: 246 PFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYV 305
             +G AW+L D   + V +EA G A      +DG + Y+ I KF + + A N+  D   +
Sbjct: 280 ALYGRAWELKDPNVNGVGAEAVGPA----TDTDGSMNYNEILKFNKQSGA-NVVYDKVAI 339

Query: 306 IGYTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDD 342
             Y+Y  TTWI Y++  +I  K+R AKSL + GYF   +  D D
Sbjct: 340 SFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKD 371

[Close]

BLAST of Moc08g43650 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 7.6e-42
Identity = 114/339 (33.63%), Postives = 182/339 (53.69%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSE------ATIINYF 65
           V+  YW    N   P ++ID + FTH+F  YA V+P  +   ++  E      AT +  F
Sbjct: 37  VKGIYWI--ENPLFPPSSIDTSLFTHIF--YAFVSPNKFTYKLEEEEEDSTTVATSLTTF 96

Query: 66  TLTVSLSNPTVKTLLSIKG---DGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQW 125
           T T     P + TLLSI G   + ++ A +AS+P  R TFI S+I+ A+   FDG+D  W
Sbjct: 97  TNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDW 156

Query: 126 LYPSSQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV---SNLPIVHHNIQYPIDT 185
            +P++ +EM +   LL +W  A+ ++  +  R  L+L AAV    N  +      YP+D+
Sbjct: 157 EFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDS 216

Query: 186 IIQTLDWVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQI 245
           I + LDWVN+ SYD     S+V  TG  S +++ K+ ++SV SG+ +WI+    +  ++I
Sbjct: 217 INKNLDWVNVMSYDLRGSGSNV--TGAPSGMFDSKS-NVSVVSGLFSWIR--GGVAPEKI 276

Query: 246 VFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKND 305
           V G+P +G +WKL D   H + +   G   G     DG + Y  +  F +   AK +  D
Sbjct: 277 VMGMPLYGKSWKLQDPNVHGIGAPNVGPGPG----VDGGMAYFQVVDFNKQMGAK-VVYD 336

Query: 306 GKYVIGYTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYF 333
            +    Y+Y  +TWI Y++  T+  K+  A++L + GYF
Sbjct: 337 KETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYF 361

[Close]

BLAST of Moc08g43650 vs. ExPASy TrEMBL
Match: A0A6J1CPI6 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.3e-197
Identity = 342/343 (99.71%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60
           MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT
Sbjct: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60

Query: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120
           LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS
Sbjct: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120

Query: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180
           SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW
Sbjct: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180

Query: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240
           VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH
Sbjct: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240

Query: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGY 300
           GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGY
Sbjct: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGY 300

Query: 301 TYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVD 344
           TYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDV+
Sbjct: 301 TYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVN 343

[Close]

BLAST of Moc08g43650 vs. ExPASy TrEMBL
Match: A0A6J1CQ27 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111013111 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 1.5e-120
Identity = 215/294 (73.13%), Postives = 248/294 (84.35%), Query Frame = 0


Query: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60
           M P  VR  YWF ST AELPSANIDA FF+HLFCGY IV+P+TY+VTIDSSE  +IN F+
Sbjct: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60

Query: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120
            TV LSNP VKTLLSI+GDG VL KM SE  NRE FI SSI+ A EG F+GLDL+WLYPS
Sbjct: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120

Query: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180
           SQ+EME FE++LIEWHSA+VEDA+NYHRQQLILVAAVSNLPIV HNIQYPIDTIIQTLDW
Sbjct: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180

Query: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240
           VNL SYDFYTP SS   TGPSSALYN KT+ +S++SGI++WI  +PNLPS++IVFGIPFH
Sbjct: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240

Query: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDG 295
           GWAWKLAD  +HD+FS+A+G A+G  ISSDG+I YSNIKKFI+ N+A+N K  G
Sbjct: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMG 294

[Close]

BLAST of Moc08g43650 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 3.5e-114
Identity = 211/341 (61.88%), Postives = 258/341 (75.66%), Query Frame = 0


Query: 4   SFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTV 63
           S V   YW +STN +LP +NIDA  FTHLFCG+A ++  +YEVT+ +S+AT+IN F+ TV
Sbjct: 9   SCVNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTV 68

Query: 64  SLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQE 123
            +SNP VKTLLSI+GD  V AKMASEP  R+ FI+SSI+ A +G F+GLDLQWLYPSS+E
Sbjct: 69  RMSNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEE 128

Query: 124 EMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNL 183
           EM+NFE LL  W  A V+DA+ YHR Q ILVA+VSNLP V  +I YP++ I Q LDWVNL
Sbjct: 129 EMQNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNL 188

Query: 184 FSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWA 243
            SYDFYTP SS   TGPSSAL+N    SLS N GI+AWI+   NLP +QIVFGIPFHGWA
Sbjct: 189 ISYDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWA 248

Query: 244 WKLADRLQHDVFSEADGAAIGHD-ISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTY 303
           WKLA+  +HDVFS+A G A G   IS +GQI  SN+K+FI+ N A+ +K+D +Y I Y Y
Sbjct: 249 WKLANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAY 308

Query: 304 FDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVD 344
             TTWIAYE+E TIP+K+  AKS  VLGYFA NIAADD+V+
Sbjct: 309 AGTTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVN 349

[Close]

BLAST of Moc08g43650 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 9.4e-112
Identity = 201/336 (59.82%), Postives = 249/336 (74.11%), Query Frame = 0


Query: 5   FVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVS 64
           FV A YW AST+  LP+ANIDA  FTHLF GYA +N  TY+VT+D S+  +I+ F+ TV 
Sbjct: 10  FVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSSTVK 69

Query: 65  LSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEE 124
           LSNP VKTLLSI  D +V A MA++P NR+ FIKSSI  A+ G FDGLDLQWLYPSSQ++
Sbjct: 70  LSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 125 MENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLF 184
           M+NF++L+  WH AVV+DA +    QL+LVA VSNLP +  NIQYP D I Q LDWVNL 
Sbjct: 130 MDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLL 189

Query: 185 SYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAW 244
           SYDFYTP SS   TGPSSA +NPK ++LS N GI +W +   NLP ++IVFGIPFHGWAW
Sbjct: 190 SYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAW 249

Query: 245 KLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFD 304
           KL +  QH +F++ADGAA G  I   GQI+YS+++KFIE N+A     D  Y++ Y Y++
Sbjct: 250 KLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYE 309

Query: 305 TTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADD 341
           TTWI YE+E TI +K+ LAK+  VLGYFA NIAADD
Sbjct: 310 TTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

[Close]

BLAST of Moc08g43650 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 3.6e-111
Identity = 199/336 (59.23%), Postives = 249/336 (74.11%), Query Frame = 0


Query: 5   FVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVS 64
           FV A YW AST+  LP+ANIDA  FTHLF GYA +N  TY+VT+D S+  +I+ F+ TV 
Sbjct: 10  FVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSSTVK 69

Query: 65  LSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEE 124
           LSNP VKTLLSI GD +V A MA++P NR+ FIKSSI  A+ G FDGLDLQWLYPSSQ++
Sbjct: 70  LSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQ 129

Query: 125 MENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLF 184
           M NF++L+  WH AVV+DA +    QL+LVA VSNLP + +NIQYP D I Q LDWVNL 
Sbjct: 130 MANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWVNLL 189

Query: 185 SYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAW 244
           SYDFYTP S    TGPSSA +NPK ++LS N GI +W +   NL  ++IVFGIPFHGWAW
Sbjct: 190 SYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHGWAW 249

Query: 245 KLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFD 304
           KLA+  QH +F++ADGAA G  I   GQI+YS+++KFIE N+A     D  Y++ Y Y++
Sbjct: 250 KLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYE 309

Query: 305 TTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADD 341
           TTWI +E+E T+ +K+ LAK+  VLGYFA NIAADD
Sbjct: 310 TTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

[Close]

BLAST of Moc08g43650 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 263.1 bits (671), Expect = 4.0e-70
Identity = 150/347 (43.23%), Postives = 215/347 (61.96%), Query Frame = 0


Query: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60
           M  + V+A+YWF +  +E P  +ID++ FTHLFC +A +N QT +VT+ S+     + FT
Sbjct: 23  MAQTVVKASYWFPA--SEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFT 82

Query: 61  LTVSLSNPTVKTLLSIKG---DGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWL 120
            TV   NP+VKTLLSI G   D +  A MAS P +R++FI SSI+ A+   F GLDL W 
Sbjct: 83  QTVQRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWE 142

Query: 121 YPSSQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV--SNLPIVHHNIQYPIDTII 180
           YPSS  EM NF +LL EW SAVV +A +  + +L+L AAV  SN    ++++ YP+  + 
Sbjct: 143 YPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSN---NYYSVLYPVSAVA 202

Query: 181 QTLDWVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVF 240
            +LDWVNL +YDFY P  S + TGP +AL++P     S ++G  +WI+    LP+++ V 
Sbjct: 203 SSLDWVNLMAYDFYGPGWS-RVTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVL 262

Query: 241 GIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGK 300
           G P++G+AW+L +   H  ++   GAA    IS DG I Y  I+KFI  N A  + N   
Sbjct: 263 GFPYYGYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVYN--S 322

Query: 301 YVIG-YTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDD 342
            V+G Y Y  T WI Y++  +I  K+R AK   +LGYF+ ++ ADD+
Sbjct: 323 TVVGDYCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN 355

[Close]

BLAST of Moc08g43650 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 252.3 bits (643), Expect = 7.0e-67
Identity = 145/339 (42.77%), Postives = 201/339 (59.29%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65
           V+A YWFA   +E P A ID++ FTHLFC +A +N  TY+V + S      + FT TV  
Sbjct: 26  VKATYWFA--ESESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 66  SNPTVKTLLSIKGD---GSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQ 125
            NPTVKTLLSI GD       A MAS P +R+ FI SSIK A+   F GLDL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 126 EEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVN 185
            EM+NF  LL EW  AV  +AR+  + +L+L AAV      ++++ +P++ +  +LDWVN
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVN 205

Query: 186 LFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGW 245
           L +YDFY   SS + T   + LY+P T   S ++G+ AW +    LP+++ V G P +G+
Sbjct: 206 LVAYDFYESGSS-RVTCSPAPLYDPITTGPSGDAGVRAWTQA--GLPAKKAVLGFPLYGY 265

Query: 246 AWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTY 305
           AW L D   H+ ++ + G A    IS DG I Y  I++FI  NKA  + N    V  Y Y
Sbjct: 266 AWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMVYN-SNLVQNYCY 325

Query: 306 FDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDD 342
              TWI Y++  +I  K++ AK   +LGYF+ +I ADD+
Sbjct: 326 AKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

[Close]

BLAST of Moc08g43650 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 245.4 bits (625), Expect = 8.6e-65
Identity = 145/341 (42.52%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 4   SFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTV 63
           S V+A+YW      + P+ NID+  FTHLFC +A V+  T+EVTI ++ +  ++ FT TV
Sbjct: 12  SIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTV 71

Query: 64  SLSNPTVKTLLSIKG---DGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 123
              N  V+TLLSI G   D +VLA MAS  KNR+ FI SSI  A++  F GLDL W YPS
Sbjct: 72  KDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPS 131

Query: 124 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 183
           +  EM NF  L+ EW +AVVE++   ++  L+L AAV   P  ++  +YP+  I   LD+
Sbjct: 132 NDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSP-DYYGEEYPVQAIADNLDF 191

Query: 184 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDS-LSVNSGIEAWIKCYPNLPSQQIVFGIPF 243
           VN+ +YDFY P  S   TGP +AL++P   +  S +SG+  W++    LP+++ V G  +
Sbjct: 192 VNIMAYDFYGPGWS-PVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKAVLGFSY 251

Query: 244 HGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIG 303
            GWAW L D   +   +  DGAA    ISSDG I Y+ I+ +I  N A    +    VIG
Sbjct: 252 CGWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAATFHDPA--VIG 311

Query: 304 -YTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAAD 340
            Y Y  TTWI Y++  +I  K+R AK   +LGYF+ ++ AD
Sbjct: 312 FYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

[Close]

BLAST of Moc08g43650 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 144/342 (42.11%), Postives = 202/342 (59.06%), Query Frame = 0


Query: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65
           V+A+YWF +T  + P+ +ID++ FTHLFC +A +  ++YE+TI +      + FT TV  
Sbjct: 7   VKASYWFPAT--DFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQQ 66

Query: 66  SNPTVKTLLSIKG---DGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQ 125
            NP VKTLLSI G   D    A MAS P +R +FI+S+I  A+   F GLDL W YP ++
Sbjct: 67  RNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRNE 126

Query: 126 EEMENFESLLIEWHSAVVEDARNYHRQQLILVAAV---SNLPIVHHNIQYPIDTIIQTLD 185
           EEM +F  LL EW SAV  ++ +     LIL AAV   SN    +  + YP+  I  +LD
Sbjct: 127 EEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSN----YQGVPYPVLAISNSLD 186

Query: 186 WVNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPF 245
           W+NL +YDFY P  S   TGP ++LY P TD  S +SG+  W +    LP+++ V G P+
Sbjct: 187 WINLMAYDFYGPGWST-VTGPPASLYLP-TDGRSGDSGVRDWTEA--GLPAKKAVLGFPY 246

Query: 246 HGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIG 305
           +GWAW LAD    DV +  D    G  IS DG+I Y  ++ +I  N A  + +D   V  
Sbjct: 247 YGWAWTLAD---PDV-NGYDANTTGPAISDDGEISYRQLQTWIVDNGATKV-HDDMMVGD 306

Query: 306 YTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDD 342
           Y Y  TTWI Y++E +I  K+  AK   +LGYF+ ++  DD+
Sbjct: 307 YCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

[Close]

BLAST of Moc08g43650 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 235.3 bits (599), Expect = 8.9e-62
Identity = 145/350 (41.43%), Postives = 202/350 (57.71%), Query Frame = 0


Query: 4   SFVRAAYWFASTNAE--------LPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATI 63
           S V+A+YWF    ++         PS+ ID+  FTHLFC +A V+  T+EVTI ++ +  
Sbjct: 12  SIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQ 71

Query: 64  INYFTLTVSLSNPTVKTLLSIKG---DGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGL 123
            + FT TV   N  V+TLLSI G   D +VLA MAS  KNR+ FI SSI  A++  F GL
Sbjct: 72  FSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGL 131

Query: 124 DLQWLYPSSQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPID 183
           DL W YPS+  EM NF  LL EW +AVVE++   ++  L+L AAV   P  +  ++YP+ 
Sbjct: 132 DLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSP-QYDGVEYPVK 191

Query: 184 TIIQTLDWVNLFSYDFYTPTSSVKCTGPSSALYNPKTD--SLSVNSGIEAWIKCYPNLPS 243
            I   LD+VN+ +YDFY P  S   TGP +AL++  ++    S NSG+  W+     LP 
Sbjct: 192 AIADNLDFVNIMAYDFYGPGWS-PVTGPPAALFHDPSNPAGRSGNSGLRKWLD-EAKLPP 251

Query: 244 QQIVFGIPFHGWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNL 303
           ++ V G P+ GWAW L D   +   +  DGAA    IS DG I Y+ I+ +I  N A   
Sbjct: 252 KKAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGAATF 311

Query: 304 KNDGKYVIG-YTYFDTTWIAYENEVTIPEKLRLAKSLHVLGYFASNIAAD 340
            +    VIG Y Y   TWI Y++  +I  K++ AK   +LGYF+ ++ AD
Sbjct: 312 HDPA--VIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGAD 352

The following BLAST results are available for this feature:

BLAST of Moc08g43650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143191.1	8.8e-197	99.71	chitotriosidase-1-like [Momordica charantia]	[more]
XP_022143178.1	3.0e-120	73.13	chitinase-3-like protein 2 [Momordica charantia]	[more]
XP_022159350.1	7.2e-114	61.88	chitinase-3-like protein 2 [Momordica charantia]	[more]
XP_023521369.1	2.3e-112	60.71	acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023545402.1	1.1e-111	60.60	acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc08g43650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	5.6e-69	43.23	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
A0A072UR65	3.6e-44	33.63	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
A0A1B1J8Z2	9.0e-43	33.24	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072VEP0	2.0e-42	33.72	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]
U5N4E3	7.6e-42	33.63	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]

BLAST of Moc08g43650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPI6	4.3e-197	99.71	chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1	[more]
A0A6J1CQ27	1.5e-120	73.13	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111013111 PE=4 S...	[more]
A0A6J1DYG4	3.5e-114	61.88	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]
A0A6J1G2P4	9.4e-112	59.82	acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE...	[more]
A0A6J1KB82	3.6e-111	59.23	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1	[more]

BLAST of Moc08g43650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	4.0e-70	43.23	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	7.0e-67	42.77	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	8.6e-65	42.52	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	1.4e-62	42.11	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	8.9e-62	41.43	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 5..339 e-value: 3.6E-58 score: 209.2
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 239..310 e-value: 4.1E-75 score: 255.2
IPR029070	Chitinase insertion domain superfamily	SUPERFAMILY	54556	Chitinase insertion domain	coord: 240..310
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..340 e-value: 4.1E-75 score: 255.2
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 4..342
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 4..342
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 9..338 e-value: 4.5E-52 score: 177.7
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 5..353 score: 38.202759
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 7..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43650.1	Moc08g43650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0016787	hydrolase activity