Moc08g43050 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g43050
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H-like domain containing protein
Location	chr8: 33071849 .. 33075321 (-)
RNA-Seq Expression	Moc08g43050
Synteny	Moc08g43050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAGTTCGAATGGTTGGACTACTGAGAGTTACTGGTCGCCTCCTCCTGACTGGTTGGATTAAAATAAACGTGGATGTAGCATGCAAGAAACATCAATTTAGAACTGCAATAGGGATTGTTTGCAGAAATGAAAAGGGGCAAATTTTGGCAGCAGCATCTCGTAGAATGGAGGCTTACCATGATCCGCTAATGGCAGAATCTCTTGCTCTCCTTGAAGGCCTGCGTCTGGCTGACCATCTGAATATCCAACAAGTACAGTTTGAATCAGATTCTTTGCAATTGATCAATTTGCTAACTGAGGATCCTATTTCTTGGTGCGGTTCCAATGTCTGGTTAGAAGATATACGTGTATTGGCGAGAAATTTCATTGATTTCAATTTTTTGCATGTCTGTCGGTCCTCAAACAAGGCAGCCCGCAGGGTTGCTTCTTTTGCTGTTGGTTTTGATAATCCCTGTTTTTGGGCTCTGATTTTCCTGAATGGTTAATGGATGTAACCACTCAGGATATGTTATGTTTCAATATACTAGCCATAACGGCTTTGGTTGAGTTTCAATAAAGTTCCTTTTATCCTGGTCATATTCGATCAGTTATATGGGTACCGTCCAAATTCATCGAAACCAATTTTTTCTCCTAGAAAAAAATGTCAAACAGCCTCTAACCAAGAATTTATAAGAGTTCAAATCATGCCTCGTATTAATTAGACATCTCAGGATAATCTATTAATTTTCTTGTCCGATAAGTTACAACATTTTTTAAAATATTCATCACATGAACTAAAAAACAACGACAACTTGAGCACTCAATTGGTTAAGACAATTATCTTTATTTTCCAATCAATAGACTGTGAATTTAATTTGTGCTGTACTTTGAAAAAAATAGAAATAGAAAAAAATTTGGTGCTAAAATCCAAGAGATATTGAATTCTACAAAGTATAGACCATACAAATAGGCTCAGCAAATAAGACGTTTAGGTAATTGAAAATATTTGGCTCTTTAAGACTTCTACTTGACACCTGATCATTCTATTTAAATAAAAACATTTATGCTTTGTTCGATTTCAAATTCTAGAATTCTAAATTATAGAAATGATTTCTAATTCTTAATTGCATTTAGAATCTACAAATTTTGATGGATTTTAAATTAGTTAAAAATAAAATTTTTTGTTGGATGATGGCAGGAAATGAAAATAAAATATATATTTTACCATGCCTTTATAATTATAAAACAAAATCTTAACAACATTCTTCTTATATCAAAACCCTACTCCCTTTTCTTATGAATTCTCGTTCGTATCTCATTGTCTCCTTTTTTCTTTTTTTTTTTTTTGTATTCCTCCTCTTTCTTCATCTCCCTTTTATTTCATCATCTCATTTTTTCTTTCTTCTCTTTATACCTTCTTCTCCATTTTATTTCTTTTTTTTTCTTATCTCATCTTCATTTTCTTTAGTCATTTTATCTCATCTCTCATTTTATCTCATCTCATTCTCTATGCATTGGTTTCATTTGGTCTCGTTCTTTTTTGGTTCAGTTTGATTTCTTCAATTAAACTGGTTGTAAGGGTAAAATTGTAATATAGTCAACTTTCAATTAAAATCCGATCAAATCCAATCTATTTTGGATGGAAATTTTTTTGGGACAAAACGGGATTTTAAATCATATTGGATTTCAAATTCTATTTTTTTTAATCCAACCAAACACGAAAAATGATTTTCCTCATAAATCTGAGGAACTTGTAGAGTAATTATTTGTGTTTCGCGAGTTTTTTTTCCTTTTTTATTTAAGTGATAAGAAGTCGTAAACAGTTGAAAAATCTTCTTAACATTTATCTTTTCTTATATTCGGTACCTTATATTGGGTAAGGCAGTATAAATGTGGTTTCAATTTATCTTTTCTTATTTTTTTATTTGTTGCTTGAAGTTTTGCGTTGCGTCTATTTTGATTTAATTCTGTTATAATTGATATTTCGGAACCAGAAAGATAATATTAGTGGACTGTTTTGTCCTTACCAGTAAGGATGTTTATAAAGGATGTCAACCGTTAGAACTGCTCCAACTTCTGTTCGTAGAGAATTTAACTTTTGGAGGGAAAAATATGACCCTTTTTCCTGCCTAATGAGAAAAATGAGTTTAGGCAGAAAATGGAACTAGGTCAAAATTGACAAAAGTCGGCGACGTAAGCTCGCTGACTCGGAACACTCGGAGATAACCTGCACAAGATTAGGACAAACTCTCTCACGCTCAAGTTAGTGAAACAGCTCGGACTCAATAGTAAGTTCAAGAAGAAATTTTATATCTGGTGCGAAAATAGTGCCCTCTATTTATAGGTTTGGGATTCGACAGACCTCCCGATCGACAGCGTCGAATGGCAGATCGGACATGTCGGACTCTGCATGCCAGTCCTTGGAGCAAATCAAGCTTCTTATGAGAAGAGTATAACAAATGATTGCCTAGGTCTCCATCTCGGAAGGTTTGAGGTAAAGCTGACTCAGATTATCCCTCACGTGGATATATTCCTGTCCTCCATATGACATAGCGATTTTCTTCCCTAAACAAAATGATTTATGCTAGATCACCCAAATAGATTTGTTAGATTTTTTATAGGAAAGAAAATGCTTTATGAAATATTAACTTTTGGTTTGTTAAAGACTGATAAGTGAAAGATAATTTAAAGATAAGTGAAAACTTATGTTTGGCAAGAGGTAATTTAATGACCAAAAGTTTATGCTTACTAAATAAATTCAGTCATTTTTGTCGTGTATAATATGATTGAATTTCGAGTTGGTAATATTTTATAACATGGTTTAATCATATGAAAAAGAAAGGGAAAAAAAAGGAAGGTAGGAAGGAAAAAATATTGGTTTATAATTATTTAGGTTAAATGTATGTAGCTTTAAGAAAATAAAAAAAAAAACGTAATCAACACTTGTATCTATCAAGAGGGCATGAGTTCAAATCCTCAAGCACTTCCTGTTAACAGGAAGCCACATAAACTTTAAAGAAATGGTCCTTAGCATGAGGAACAGGAGGATTAAAGATTCTCGAAGGATGATATTGAAATCTTACTAGAAAAAGAAAATCTTACTAGAAAAAGAAAGACGGAAAAAAAAAATTTAGTTTTGCAGTGAAAGTTGCGGATCTTTTCGCCCCCCTCTCCCGACTCAGTCACATGCAACCAGGCTTGAAACCCGGCCCACTCTCCCTTCTGTGCCCCCAGACCAAAAAAAAGTATGGTCTTTCTGAGAAGAAAGAAGAGCATATCTACCTTGAAGACCTTCTGGAAAGTCGCCTATGGAAAGAGCATACCCAACCAATATGAGCCTGTCCGAGACTTGCCATTCCCATGGAAGAGAGTTTCCCTGCGTATTGATTCATTACCGACTGAACGGGAAATGCTTGCTTCTATTCTTTCTCTCCGAGCGATGAACTGTGGTTGTCTAGCTGTACGGAATCCCTGGGGAGTGAAGGGATAG

mRNA sequence

ATGCAAGTTCGAATGGTTGGACTACTGAGAGTTACTGGTCGCCTCCTCCTGACTGGTTGGATTAAAATAAACGTGGATGTAGCATGCAAGAAACATCAATTTAGAACTGCAATAGGGATTGTTTGCAGAAATGAAAAGGGGCAAATTTTGGCAGCAGCATCTCGTAGAATGGAGGCTTACCATGATCCGCTAATGGCAGAATCTCTTGCTCTCCTTGAAGGCCTGCGTCTGGCTGACCATCTGAATATCCAACAAGTACAGTTTGAATCAGATTCTTTGCAATTGATCAATTTGCTAACTGAGGATCCTATTTCTTGGTGCGGTTCCAATGTCTGGTTAGAAGATATACGTGTATTGGCGAGAAATTTCATTGATTTCAATTTTTTGCATGTCTGTCGGTCCTCAAACAAGGCAGCCCGCAGGGTTGCTTCTTTTGCTGTTGGTTTTGATAATCCCTGTTTTTGGGCTCTGATTTTCCTGAATGACCTCCCGATCGACAGCGTCGAATGGCAGATCGGACATGTCGGACTCTGCATGCCAGTCCTTGGAGCAAATCAAGCTTCTTATGAGAAGAGTATAACAAATGATTGCCTAGGTCTCCATCTCGGAAGAAAGAAGAGCATATCTACCTTGAAGACCTTCTGGAAAGTCGCCTATGGAAAGAGCATACCCAACCAATATGAGCCTGTCCGAGACTTGCCATTCCCATGGAAGAGAGTTTCCCTGCGTATTGATTCATTACCGACTGAACGGGAAATGCTTGCTTCTATTCTTTCTCTCCGAGCGATGAACTGTGGTTGTCTAGCTGTACGGAATCCCTGGGGAGTGAAGGGATAG

Coding sequence (CDS)

ATGCAAGTTCGAATGGTTGGACTACTGAGAGTTACTGGTCGCCTCCTCCTGACTGGTTGGATTAAAATAAACGTGGATGTAGCATGCAAGAAACATCAATTTAGAACTGCAATAGGGATTGTTTGCAGAAATGAAAAGGGGCAAATTTTGGCAGCAGCATCTCGTAGAATGGAGGCTTACCATGATCCGCTAATGGCAGAATCTCTTGCTCTCCTTGAAGGCCTGCGTCTGGCTGACCATCTGAATATCCAACAAGTACAGTTTGAATCAGATTCTTTGCAATTGATCAATTTGCTAACTGAGGATCCTATTTCTTGGTGCGGTTCCAATGTCTGGTTAGAAGATATACGTGTATTGGCGAGAAATTTCATTGATTTCAATTTTTTGCATGTCTGTCGGTCCTCAAACAAGGCAGCCCGCAGGGTTGCTTCTTTTGCTGTTGGTTTTGATAATCCCTGTTTTTGGGCTCTGATTTTCCTGAATGACCTCCCGATCGACAGCGTCGAATGGCAGATCGGACATGTCGGACTCTGCATGCCAGTCCTTGGAGCAAATCAAGCTTCTTATGAGAAGAGTATAACAAATGATTGCCTAGGTCTCCATCTCGGAAGAAAGAAGAGCATATCTACCTTGAAGACCTTCTGGAAAGTCGCCTATGGAAAGAGCATACCCAACCAATATGAGCCTGTCCGAGACTTGCCATTCCCATGGAAGAGAGTTTCCCTGCGTATTGATTCATTACCGACTGAACGGGAAATGCTTGCTTCTATTCTTTCTCTCCGAGCGATGAACTGTGGTTGTCTAGCTGTACGGAATCCCTGGGGAGTGAAGGGATAG

Protein sequence

MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLNDLPIDSVEWQIGHVGLCMPVLGANQASYEKSITNDCLGLHLGRKKSISTLKTFWKVAYGKSIPNQYEPVRDLPFPWKRVSLRIDSLPTEREMLASILSLRAMNCGCLAVRNPWGVKG

Homology

[Close]

BLAST of Moc08g43050 vs. NCBI nr
Match: XP_022142956.1 (uncharacterized protein LOC111012950 [Momordica charantia])

HSP 1 Score: 327.0 bits (837), Expect = 1.6e-85
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAY 60
           MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAY
Sbjct: 13  MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAY 72

Query: 61  HDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLA 120
           HDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLA
Sbjct: 73  HDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLA 132

Query: 121 RNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLN 162
           RNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLN
Sbjct: 133 RNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLN 173

[Close]

BLAST of Moc08g43050 vs. NCBI nr
Match: XP_022154440.1 (uncharacterized protein LOC111021711 [Momordica charantia])

HSP 1 Score: 130.2 bits (326), Expect = 2.8e-26
Identity = 63/148 (42.57%), Postives = 96/148 (64.86%), Query Frame = 0


Query: 20  WIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLAD 79
           W+KINVD ACK    RT IG+VC+N+KG+I+ A  R+++ +++PL  E+LA+LEGL L  
Sbjct: 135 WVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFYEPLQVETLAILEGLWLTQ 194

Query: 80  HLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAA 139
            L++  VQ +SDS   INL++++       NVW++DI + A NFID  F+H+ RSS+   
Sbjct: 195 CLDLIFVQVDSDSQNSINLISQEYDPSHQPNVWVDDIHMCAINFIDILFMHIQRSSSNVT 254

Query: 140 RRVASFAVGFDNPCFWALIFLNDLPIDS 168
            R+AS A+  +    W   F N L +++
Sbjct: 255 HRLASTAISSNPNKIWISRFPNWLTVEA 282

[Close]

BLAST of Moc08g43050 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 92.4 bits (228), Expect = 6.5e-15
Identity = 46/56 (82.14%), Postives = 47/56 (83.93%), Query Frame = 0


Query: 19  GWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEG 75
           GWIKINVD ACKKHQFRT IGIVCRNEKGQILAAAS      HDPLMAESLAL +G
Sbjct: 159 GWIKINVDAACKKHQFRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

[Close]

BLAST of Moc08g43050 vs. NCBI nr
Match: XP_018850378.1 (uncharacterized protein LOC109012930 [Juglans regia] >KAF5441962.1 hypothetical protein F2P56_037084 [Juglans regia])

HSP 1 Score: 88.6 bits (218), Expect = 9.3e-14
Identity = 47/144 (32.64%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 21  IKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLADH 80
           IK N D A  K   R  IG++ RN +G I+A+    M+ + DPL+ E++A          
Sbjct: 17  IKANWDAAIDKANSRVGIGVIVRNSEGAIMASLCSSMDLFPDPLLGEAIAARRASSFCAD 76

Query: 81  LNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAAR 140
           L  Q V  E DSL ++  +     SW  S + + DI++L   F+ ++ LHV R  N  A 
Sbjct: 77  LGFQHVILEGDSLLVVKAIQNKEDSWSDSGLVIRDIKILLSKFLSWSILHVHREVNVIAH 136

Query: 141 RVASFAVGFDNPCFWALIFLNDLP 165
            +A FA+     C    I + D P
Sbjct: 137 HLAKFALSCQEDC----IMIEDCP 156

[Close]

BLAST of Moc08g43050 vs. NCBI nr
Match: KAG2717700.1 (hypothetical protein I3760_03G188300 [Carya illinoinensis] >KAG6722946.1 hypothetical protein I3842_03G186800 [Carya illinoinensis])

HSP 1 Score: 88.6 bits (218), Expect = 9.3e-14
Identity = 47/135 (34.81%), Postives = 75/135 (55.56%), Query Frame = 0


Query: 21  IKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLADH 80
           +K+NVD A  K Q+R  +GI+ R+  G +L AAS++    +DP   E LA+L GL+L   
Sbjct: 304 LKLNVDGAIFKEQYRAGVGIILRDTNGDVLLAASKKESMVNDPAEVELLAMLRGLQLVLP 363

Query: 81  LNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAAR 140
           L I+++  ESDSL ++  L ++  SW      +++ ++L   F      HV R  N+AA 
Sbjct: 364 LGIEELIIESDSLVMVTQLQQEDESWSVLGNLIKETKMLMAWFKGCTIQHVGRLGNEAAH 423

Query: 141 RVASFAVGFDNPCFW 156
           R+  FA   ++   W
Sbjct: 424 RLGKFAWHIEDISIW 438

[Close]

BLAST of Moc08g43050 vs. ExPASy TrEMBL
Match: A0A6J1CNR4 (uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012950 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 7.5e-86
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAY 60
           MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAY
Sbjct: 13  MQVRMVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAY 72

Query: 61  HDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLA 120
           HDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLA
Sbjct: 73  HDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLA 132

Query: 121 RNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLN 162
           RNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLN
Sbjct: 133 RNFIDFNFLHVCRSSNKAARRVASFAVGFDNPCFWALIFLN 173

[Close]

BLAST of Moc08g43050 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.4e-26
Identity = 63/148 (42.57%), Postives = 96/148 (64.86%), Query Frame = 0


Query: 20  WIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLAD 79
           W+KINVD ACK    RT IG+VC+N+KG+I+ A  R+++ +++PL  E+LA+LEGL L  
Sbjct: 135 WVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFYEPLQVETLAILEGLWLTQ 194

Query: 80  HLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAA 139
            L++  VQ +SDS   INL++++       NVW++DI + A NFID  F+H+ RSS+   
Sbjct: 195 CLDLIFVQVDSDSQNSINLISQEYDPSHQPNVWVDDIHMCAINFIDILFMHIQRSSSNVT 254

Query: 140 RRVASFAVGFDNPCFWALIFLNDLPIDS 168
            R+AS A+  +    W   F N L +++
Sbjct: 255 HRLASTAISSNPNKIWISRFPNWLTVEA 282

[Close]

BLAST of Moc08g43050 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 3.1e-15
Identity = 46/56 (82.14%), Postives = 47/56 (83.93%), Query Frame = 0


Query: 19  GWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEG 75
           GWIKINVD ACKKHQFRT IGIVCRNEKGQILAAAS      HDPLMAESLAL +G
Sbjct: 159 GWIKINVDAACKKHQFRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

[Close]

BLAST of Moc08g43050 vs. ExPASy TrEMBL
Match: A0A2I4H2K4 (uncharacterized protein LOC109012930 OS=Juglans regia OX=51240 GN=LOC109012930 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 4.5e-14
Identity = 47/144 (32.64%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 21  IKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLADH 80
           IK N D A  K   R  IG++ RN +G I+A+    M+ + DPL+ E++A          
Sbjct: 17  IKANWDAAIDKANSRVGIGVIVRNSEGAIMASLCSSMDLFPDPLLGEAIAARRASSFCAD 76

Query: 81  LNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAAR 140
           L  Q V  E DSL ++  +     SW  S + + DI++L   F+ ++ LHV R  N  A 
Sbjct: 77  LGFQHVILEGDSLLVVKAIQNKEDSWSDSGLVIRDIKILLSKFLSWSILHVHREVNVIAH 136

Query: 141 RVASFAVGFDNPCFWALIFLNDLP 165
            +A FA+     C    I + D P
Sbjct: 137 HLAKFALSCQEDC----IMIEDCP 156

[Close]

BLAST of Moc08g43050 vs. ExPASy TrEMBL
Match: A0A803QG78 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.7e-13
Identity = 46/136 (33.82%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 21   IKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLADH 80
            +K+N+D A  +    T +G V R+  G ++AA S+++    +P   E+LAL  G+  A H
Sbjct: 1168 VKLNIDAAVDRSTKVTGLGAVFRDSMGMVVAAFSKKLLGSFEPHEMEALALFHGVHYALH 1227

Query: 81   LNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAAR 140
                Q+Q E+D+L++ N L     +    N  + D+  L   F + N  HV R++N AA 
Sbjct: 1228 FQFPQLQIETDALRVKNALYIPTAACSTFNDIIMDVSSLLSFFPNVNVSHVKRTANTAAH 1287

Query: 141  RVASFAVGFDNPCFWA 157
             +A FA+G D  CFW+
Sbjct: 1288 GLAKFALGVDEDCFWS 1303

[Close]

BLAST of Moc08g43050 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-11
Identity = 42/132 (31.82%), Postives = 60/132 (45.45%), Query Frame = 0


Query: 20  WIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLAD 79
           W+K N D    +   R  IG V RNEKG++    +R +      L AE  A+   +    
Sbjct: 427 WVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLS 486

Query: 80  HLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAA 139
                 V FESDS  LI +L  D I W      ++D++ L   F +  F+ + R  N  A
Sbjct: 487 RFQYNYVIFESDSQVLIEILNNDEI-WPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLA 546

Query: 140 RRVASFAVGFDN 152
            RVA  ++ F N
Sbjct: 547 ERVARESLSFLN 557

[Close]

BLAST of Moc08g43050 vs. TAIR 10
Match: AT2G13980.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 7.6e-06
Identity = 39/136 (28.68%), Postives = 61/136 (44.85%), Query Frame = 0


Query: 21  IKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLADH 80
           IK N D         +    + RN  G      S +++    PL AE+ ALL  L+    
Sbjct: 21  IKCNYDAGFNVQNLDSTARWIIRNHDGIAQHWGSLQLDNTSTPLEAETKALLAALQQTWI 80

Query: 81  LNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKAAR 140
               +V  E D   L NL++        +N+ L+DIR+ A+ F +  F  V R  NK A 
Sbjct: 81  RGYLRVIMEGDCETLTNLVSGSSSHNHLANL-LDDIRIWAKKFSNVQFSFVRRGGNKVAH 140

Query: 141 RVASFAVGFDNPCFWA 157
            +A   +G ++ CF++
Sbjct: 141 ELAK--LGCNSTCFYS 153

[Close]

BLAST of Moc08g43050 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 4.9e-05
Identity = 30/81 (37.04%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 63  PLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARN 122
           PLMAE++AL   L+ A  + I ++   SDS QLI  +T +  S     + + DI  L+  
Sbjct: 73  PLMAEAIALFLALQYAQSIGITKLSMASDSQQLITAITSESPSTEFYGI-IFDILNLSLG 132

Query: 123 FIDFNFLHVCRSSNKAARRVA 144
           F D +F  V RS N+ A  +A
Sbjct: 133 FADVSFSFVPRSENRVADELA 152

[Close]

BLAST of Moc08g43050 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 8.4e-05
Identity = 36/121 (29.75%), Postives = 59/121 (48.76%), Query Frame = 0


Query: 20  WIKINVDVACKKHQFRTA-IGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRLA 79
           WIK N D A   HQ R A  G + R+E G  + +         D L +E  AL+  ++ A
Sbjct: 193 WIKYNYDGAF-NHQTRNAKAGWLMRDENGVYMGSGQAIGSTTSDSLESEFQALIIAMQHA 252

Query: 80  DHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWLEDIRVLARNFIDFNFLHVCRSSNKA 139
                ++V FE DS Q+  L+  + +++ G   W+ + R   + F +  F  V R++N+ 
Sbjct: 253 WSQGYRKVIFEGDSKQVEELMNNEKLNF-GRFNWIREGRFWQKRFEEAVFKWVPRTNNQP 311

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BLAST of Moc08g43050 vs. TAIR 10
Match: AT5G38920.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.4e-04
Identity = 29/95 (30.53%), Postives = 51/95 (53.68%), Query Frame = 0


Query: 64  LMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTE-------DPISWCGSNVWLEDI 123
           L+ E+ A+   +     LN ++V FESDS QL+++L +       DPI        L+DI
Sbjct: 84  LVTEAEAMRWAVLSLSRLNYRKVIFESDSQQLVSILVDNGDMPILDPI--------LQDI 143

Query: 124 RVLARNFIDFNFLHVCRSSNKAARRVASFAVGFDN 152
           ++L ++F +  F+ + R  N  A R+A  ++  +N
Sbjct: 144 KLLLQHFEETKFVFMHRGGNGVADRIAKESLSLEN 170

The following BLAST results are available for this feature:

BLAST of Moc08g43050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142956.1	1.6e-85	100.00	uncharacterized protein LOC111012950 [Momordica charantia]	[more]
XP_022154440.1	2.8e-26	42.57	uncharacterized protein LOC111021711 [Momordica charantia]	[more]
XP_022145060.1	6.5e-15	82.14	uncharacterized protein LOC111014578 [Momordica charantia]	[more]
XP_018850378.1	9.3e-14	32.64	uncharacterized protein LOC109012930 [Juglans regia] >KAF5441962.1 hypothetical ...	[more]
KAG2717700.1	9.3e-14	34.81	hypothetical protein I3760_03G188300 [Carya illinoinensis] >KAG6722946.1 hypothe...	[more]

BLAST of Moc08g43050 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc08g43050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNR4	7.5e-86	100.00	uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DJL6	1.4e-26	42.57	uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1CTE3	3.1e-15	82.14	uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A2I4H2K4	4.5e-14	32.64	uncharacterized protein LOC109012930 OS=Juglans regia OX=51240 GN=LOC109012930 P...	[more]
A0A803QG78	1.7e-13	33.82	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Moc08g43050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	1.6e-11	31.82	Ribonuclease H-like superfamily protein	[more]
AT2G13980.1	7.6e-06	28.68	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G09490.1	4.9e-05	37.04	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G25270.1	8.4e-05	29.75	Ribonuclease H-like superfamily protein	[more]
AT5G38920.1	1.4e-04	30.53	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 24..146 e-value: 2.7E-24 score: 85.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 20..149 e-value: 2.7E-16 score: 61.7
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 18..156
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 23..143 e-value: 2.89739E-26 score: 97.77
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 19..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g43050.1	Moc08g43050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity