Moc08g42110 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g42110
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Kunitz-type trypsin inhibitor
Location	chr8: 32301923 .. 32302766 (+)
RNA-Seq Expression	Moc08g42110
Synteny	Moc08g42110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATGTTATTGCAGAAGCCACTTGCCATCTTCAGCTGCCTATTCCTCTTCATGGCCATATATTCCACAGCCCAATCTCCTCCGGTGCTCGACACCGACGGCCGTCCTCTGCAGCGCGGTGTCGAGTACTTCATCAAGCCCGCCGTCACCGACGTTGCTGGCAACCTTACCCTGTTAACCCGCAAAGGTGATCCTTGCCCGTTCCATGTCGGTCAAGTACCTCTTCGTTCGACAGATATAGGTATATATTAAACTACTATGAACTCAAAAAAATTACAATCATATTTAATCTTTTACACGTATACTTAACCCTCCTCTTTATATCTGGACCGGATATTAATTTGATTCATAATTTCAACCTGTGAATTATTAATATAAATGGTATTACAGATATTTGCATTTGTCTTTCTAAGAGCTACTTATACCAATAAAAATAATTATCCTCATCCCTATTTGCAAACCCAACAATTATGATTTAATATTTTATTATATTCTCAATCTTAGGTTTTTGGCGGGTGAAGATACAATAAGAGAAGGAAGAGATTTGAAGGTCGTGTTCCAAGCGTTTTCAATTTGCATAACGTCGACTGCATGGAGAGTGGGCGAGACGGAGCCTACCACTGGAAGAAGGTTGGTGGCGACCGGGAACGACGATACCGCCACCGGGTTCTTCAGGATCGACAAGAGCGACCTTGGAGTTTACAATCTGGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGGTTGAGAAAGGAGTGAGGTTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTCGAGTTTGTGAGAGCTTAA

mRNA sequence

ATGATGATGTTATTGCAGAAGCCACTTGCCATCTTCAGCTGCCTATTCCTCTTCATGGCCATATATTCCACAGCCCAATCTCCTCCGGTGCTCGACACCGACGGCCGTCCTCTGCAGCGCGGTGTCGAGTACTTCATCAAGCCCGCCGTCACCGACGTTGCTGGCAACCTTACCCTGTTAACCCGCAAAGGTGATCCTTGCCCGTTCCATGTCGGTCAAGTACCTCTTCGTTCGACAGATATAGATACAATAAGAGAAGGAAGAGATTTGAAGGTCGTGTTCCAAGCGTTTTCAATTTGCATAACGTCGACTGCATGGAGAGTGGGCGAGACGGAGCCTACCACTGGAAGAAGGTTGGTGGCGACCGGGAACGACGATACCGCCACCGGGTTCTTCAGGATCGACAAGAGCGACCTTGGAGTTTACAATCTGGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGGTTGAGAAAGGAGTGAGGTTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTCGAGTTTGTGAGAGCTTAA

Coding sequence (CDS)

ATGATGATGTTATTGCAGAAGCCACTTGCCATCTTCAGCTGCCTATTCCTCTTCATGGCCATATATTCCACAGCCCAATCTCCTCCGGTGCTCGACACCGACGGCCGTCCTCTGCAGCGCGGTGTCGAGTACTTCATCAAGCCCGCCGTCACCGACGTTGCTGGCAACCTTACCCTGTTAACCCGCAAAGGTGATCCTTGCCCGTTCCATGTCGGTCAAGTACCTCTTCGTTCGACAGATATAGATACAATAAGAGAAGGAAGAGATTTGAAGGTCGTGTTCCAAGCGTTTTCAATTTGCATAACGTCGACTGCATGGAGAGTGGGCGAGACGGAGCCTACCACTGGAAGAAGGTTGGTGGCGACCGGGAACGACGATACCGCCACCGGGTTCTTCAGGATCGACAAGAGCGACCTTGGAGTTTACAATCTGGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGGTTGAGAAAGGAGTGAGGTTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTCGAGTTTGTGAGAGCTTAA

Protein sequence

MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDPCPFHVGQVPLRSTDIDTIREGRDLKVVFQAFSICITSTAWRVGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGVRLLALDGSAFPFEFVRA

Homology

[Close]

BLAST of Moc08g42110 vs. NCBI nr
Match: XP_022150807.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 376.7 bits (966), Expect = 1.2e-100
Identity = 187/200 (93.50%), Postives = 187/200 (93.50%), Query Frame = 0


Query: 1   MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 60
           MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL
Sbjct: 1   MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDPCPFHVGQVPLRSTDI             DTIREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 180
           VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE
Sbjct: 121 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 180

Query: 181 KGVRLLALDGSAFPFEFVRA 188
           KGVRLLALDGSAFPFEFVRA
Sbjct: 181 KGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Moc08g42110 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 317.8 bits (813), Expect = 6.4e-83
Identity = 156/198 (78.79%), Postives = 168/198 (84.85%), Query Frame = 0


Query: 3   MLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTR 62
           M+L K L  FS L L MA+ S AQSPPVLDT+GRPLQ  VEYFIKPAVTDVAGNLTLLTR
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 60

Query: 63  KGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWRVG 122
           KGD CPFHVGQVPLRS +I             DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 120

Query: 123 ETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKG 182
           ET+PTTGRR V TG+DDTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG
Sbjct: 121 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 180

Query: 183 VRLLALDGSAFPFEFVRA 188
           +RLLALDGSAFPFEFVRA
Sbjct: 181 LRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Moc08g42110 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 316.2 bits (809), Expect = 1.9e-82
Identity = 155/197 (78.68%), Postives = 167/197 (84.77%), Query Frame = 0


Query: 4   LLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRK 63
           +L K L  FS L L MA+ S AQSPPVLDT+GRPLQ  VEYFIKPAVTDVAGNLTLLTRK
Sbjct: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60

Query: 64  GDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWRVGE 123
           GD CPFHVGQVPLRS +I             DTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120

Query: 124 TEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGV 183
           T+PTTGRR V TG+DDTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG+
Sbjct: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180

Query: 184 RLLALDGSAFPFEFVRA 188
           RLLALDGSAFPFEFVRA
Sbjct: 181 RLLALDGSAFPFEFVRA 197

[Close]

BLAST of Moc08g42110 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.1 bits (806), Expect = 4.1e-82
Identity = 153/198 (77.27%), Postives = 168/198 (84.85%), Query Frame = 0


Query: 3   MLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTR 62
           M+L K L  FS L L MA+ S AQSPPVLDT+GRPLQ  VEYFIKPA+TDVAGNLTLLTR
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTR 60

Query: 63  KGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWRVG 122
           KGD CPFHVGQVPLRS +I             DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 120

Query: 123 ETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKG 182
           ET+PTTGRR V TG++DTATG+FRID+ + G+YN+GWCPSDVPIRGRPRCGRAGIL+EKG
Sbjct: 121 ETDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKG 180

Query: 183 VRLLALDGSAFPFEFVRA 188
           +RLLALDGSAFPFEFVRA
Sbjct: 181 LRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Moc08g42110 vs. NCBI nr
Match: KAG6586275.1 (hypothetical protein SDJN03_19008, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 148/182 (81.32%), Postives = 159/182 (87.36%), Query Frame = 0


Query: 19  MAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDPCPFHVGQVPLRS 78
           MA+ S AQSPPVLDT+GRPLQ  VEYFIKPAVTDVAGNLTLLTRKGD CPFHVGQVPLRS
Sbjct: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 60

Query: 79  TDI-------------DTIREGRDLKVVFQAFSICITSTAWRVGETEPTTGRRLVATGND 138
            +I             DTIREGRDLKVVFQAFSICITSTAWRVGET+PTTGRR V TG+D
Sbjct: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 120

Query: 139 DTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGVRLLALDGSAFPFEFV 188
           DTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG+RLLALDGSAFPFEFV
Sbjct: 121 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180

[Close]

BLAST of Moc08g42110 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 8.8e-35
Identity = 79/192 (41.15%), Postives = 116/192 (60.42%), Query Frame = 0


Query: 11  IFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDPCPFH 70
           IF    +++ + +T+ +  V+DT G P++   +YFI+PA+T   G+LTL+TR  + CPF+
Sbjct: 8   IFILANVWLLVVTTSIAQFVIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFN 67

Query: 71  VGQVP-------------LRSTDIDTIREGRDLKVVFQAFSICITSTAWRVGETEPTTGR 130
           VG  P             + + + D +R GRDL+V+FQA + C  ST WR+GE + TTGR
Sbjct: 68  VGLDPDAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGR 127

Query: 131 RLVATGNDDTATG----FFRIDKS-DLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGVRL 185
           R + TG DD+  G    FFRI ++   G++N+ WCP++V    +  CG  GI+ E G  L
Sbjct: 128 RFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKIL 187

[Close]

BLAST of Moc08g42110 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-31
Identity = 76/196 (38.78%), Postives = 107/196 (54.59%), Query Frame = 0


Query: 7   KPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDP 66
           + L IF    +++ + +T+ +  V+DT G P++   EYFI+PA+T   G  TL+T  G P
Sbjct: 4   RSLTIFILAHVWLLMATTSIAQFVIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-P 63

Query: 67  CPFHVGQVPLRST--------------DIDTIREGRDLKVVFQAFSICITSTAWRVGETE 126
           CP HVG      T              D D +R  RDL+V F   + C  ST WR+GE +
Sbjct: 64  CPLHVGLDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKD 123

Query: 127 PTTGRRLVATGNDDTA---TGFFRIDKSDL-GVYNLGWCPSDVPIRGRPRCGRAGILVEK 185
            T+GRRL+ TG D+ A     FFRI ++   G YN+ WCP++     + +CG  G++ E 
Sbjct: 124 ATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIREN 183

[Close]

BLAST of Moc08g42110 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-30
Identity = 79/202 (39.11%), Postives = 112/202 (55.45%), Query Frame = 0


Query: 2   MMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLT 61
           M L  + L I + + LF+   + AQ   VLDT G P++   EYFI+P +T+  G  T+++
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVS 62

Query: 62  RKGDPCPFHVG--------QVPLRST------DIDTIREGRDLKVVFQAFSICITSTAWR 121
           R  + CP HVG         + ++ T      D D +R  RDL++ FQA S C+ ST WR
Sbjct: 63  R-NESCPLHVGLELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWR 122

Query: 122 VGETEPTTGRRLVATGNDDTATG----FFRIDKSDL-GVYNLGWCPSDVPIRGRPRCGRA 181
           +GE +  +GRRL+ TG D    G    FFRI ++ L G+YN+ WCP++V    +  CG  
Sbjct: 123 LGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTV 182

Query: 182 GILVEKGVRLLALDGSAFPFEF 185
            +L E G  LLALDG   P  F
Sbjct: 183 DMLNENGKILLALDGGPLPLVF 201

[Close]

BLAST of Moc08g42110 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-15
Identity = 70/214 (32.71%), Postives = 100/214 (46.73%), Query Frame = 0


Query: 7   KPLAIFSCLFLFM-AIYSTAQSP----------PVLDTDGRPLQRGVEYFIKPAVTDVAG 66
           K L + S  F F+ A+ + A +P          PVLD DG  L+ G  Y+I P + D  G
Sbjct: 2   KELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGG 61

Query: 67  NLTL--LTRKGD-PCPFHVGQV--------PL----RSTDIDTIREGRDLKVVFQAFSIC 126
            LT+   T  G   CP  V Q         PL     +   D +R   DL + F AF  C
Sbjct: 62  GLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPC 121

Query: 127 --ITSTAWRVGETEPTTGRRLVATG------NDDTATGFFRIDK-SDLGVYNLGWCPSDV 185
              +ST WR+ + + +TG+  V  G        +T + +F+I++    G Y L +CP+ V
Sbjct: 122 RWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT-V 181

[Close]

BLAST of Moc08g42110 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.5e-15
Identity = 57/196 (29.08%), Postives = 88/196 (44.90%), Query Frame = 0


Query: 12  FSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDPCPFHV 71
           F  +    A+ ++     V+D DG  +     Y++ P +    G LTL  R G PCP+ +
Sbjct: 12  FYLVLALTAVLASNAYGAVVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGRGGQPCPYDI 71

Query: 72  GQ--------VPLRSTD----IDTIREGRDLKVVFQ-AFSICITSTAWRVGETEPTTGRR 131
            Q        +P++ ++    +  + E ++L +      +ICI ST WRVGE +    + 
Sbjct: 72  VQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQY 131

Query: 132 LVATG------NDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILV-EKGVR 188
            V  G        D+   FF+I+KS    Y   +CP      G P+C   GI + E GVR
Sbjct: 132 FVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVR 191

[Close]

BLAST of Moc08g42110 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 5.6e-101
Identity = 187/200 (93.50%), Postives = 187/200 (93.50%), Query Frame = 0


Query: 1   MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 60
           MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL
Sbjct: 1   MMMLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDPCPFHVGQVPLRSTDI             DTIREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 180
           VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE
Sbjct: 121 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 180

Query: 181 KGVRLLALDGSAFPFEFVRA 188
           KGVRLLALDGSAFPFEFVRA
Sbjct: 181 KGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Moc08g42110 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.1e-83
Identity = 156/198 (78.79%), Postives = 168/198 (84.85%), Query Frame = 0


Query: 3   MLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTR 62
           M+L K L  FS L L MA+ S AQSPPVLDT+GRPLQ  VEYFIKPAVTDVAGNLTLLTR
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 60

Query: 63  KGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWRVG 122
           KGD CPFHVGQVPLRS +I             DTIREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 120

Query: 123 ETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKG 182
           ET+PTTGRR V TG+DDTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG
Sbjct: 121 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 180

Query: 183 VRLLALDGSAFPFEFVRA 188
           +RLLALDGSAFPFEFVRA
Sbjct: 181 LRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Moc08g42110 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.4e-80
Identity = 151/198 (76.26%), Postives = 164/198 (82.83%), Query Frame = 0


Query: 3   MLLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTR 62
           M+L K L  FS L L MA  S AQSPPVLD +GRPL+  VEYFIKPA+TDVAGNLTLLTR
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTR 60

Query: 63  KGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTAWRVG 122
           KGD CPFHVGQVPLRS +I             D IREGRDLKVVFQAFSICITSTAWRVG
Sbjct: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVG 120

Query: 123 ETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKG 182
           E +PTTGRR V TG+DDTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG
Sbjct: 121 EADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 180

Query: 183 VRLLALDGSAFPFEFVRA 188
           +RLLALDGSAFPFEFVRA
Sbjct: 181 LRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Moc08g42110 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.4e-74
Identity = 142/201 (70.65%), Postives = 160/201 (79.60%), Query Frame = 0


Query: 1   MMMLLQKPLAIFSCLFLFMAIYSTAQ--SPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLT 60
           MM   +  +A+FS L  FMAI STAQ   PPVLDT+GRPLQR VEYFIKPA+TDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LLTRKGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITSTA 120
           L+TRKGD CPF+VGQVPL S +I             DTIREGRDLK VFQAF+ICIT T 
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WRVGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGIL 180
           W+VGE +P +GRR V  GND+TATG+FRIDKS+LGVYN+GWCPSDVP +GRPRCG AGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 VEKGVRLLALDGSAFPFEFVR 187
           +EKGVR LALDG AFPFEFVR
Sbjct: 181 IEKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of Moc08g42110 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.1e-72
Identity = 141/202 (69.80%), Postives = 155/202 (76.73%), Query Frame = 0


Query: 1   MMMLLQKPLAIFSCLFLFMAIYSTA---QSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNL 60
           MM   +   AIFS L  FMAI STA     PPVLDT+GRPLQR  EYFIKPA+TDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLLTRKGDPCPFHVGQVPLRSTDI-------------DTIREGRDLKVVFQAFSICITST 120
           TL+TRKGD CPF+VGQVPL S +              DTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 AWRVGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGI 180
            W+VGE +P TGRR V  G D+TATG+FRIDKSDLGVYN+GWCPSDVPI+GRPRCG AGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LVEKGVRLLALDGSAFPFEFVR 187
           L+EKGVR LALDG AFPFEFVR
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVR 202

[Close]

BLAST of Moc08g42110 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 83.2 bits (204), Expect = 2.5e-16
Identity = 57/196 (29.08%), Postives = 88/196 (44.90%), Query Frame = 0


Query: 12  FSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDPCPFHV 71
           F  +    A+ ++     V+D DG  +     Y++ P +    G LTL  R G PCP+ +
Sbjct: 12  FYLVLALTAVLASNAYGAVVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGRGGQPCPYDI 71

Query: 72  GQ--------VPLRSTD----IDTIREGRDLKVVFQ-AFSICITSTAWRVGETEPTTGRR 131
            Q        +P++ ++    +  + E ++L +      +ICI ST WRVGE +    + 
Sbjct: 72  VQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQY 131

Query: 132 LVATG------NDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILV-EKGVR 188
            V  G        D+   FF+I+KS    Y   +CP      G P+C   GI + E GVR
Sbjct: 132 FVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVR 191

The following BLAST results are available for this feature:

BLAST of Moc08g42110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150807.1	1.2e-100	93.50	miraculin-like [Momordica charantia]	[more]
XP_022938134.1	6.4e-83	78.79	alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata]	[more]
KAG7021121.1	1.9e-82	78.68	hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023521354.1	4.1e-82	77.27	alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381...	[more]
KAG6586275.1	1.3e-80	81.32	hypothetical protein SDJN03_19008, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Moc08g42110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	8.8e-35	41.15	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	1.2e-31	38.78	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV1	2.2e-30	39.11	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P13087	1.2e-15	32.71	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q8RXD5	3.5e-15	29.08	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]

BLAST of Moc08g42110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBS1	5.6e-101	93.50	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1	[more]
A0A6J1FD70	3.1e-83	78.79	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	2.4e-80	76.26	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]
A0A0A0LGB4	3.4e-74	70.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A5A7V7Y2	1.1e-72	69.80	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]

BLAST of Moc08g42110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	2.5e-16	29.08	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 29..187 e-value: 1.5E-19 score: 81.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 30..185 e-value: 3.3E-31 score: 108.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 9..187
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 30..46
None	No IPR available	GENE3D	2.80.10.50		coord: 27..187 e-value: 1.3E-33 score: 118.0
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 9..187
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g42110.1	Moc08g42110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity