Moc08g42100 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g42100
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	miraculin-like
Location	chr8: 32299276 .. 32299869 (+)
RNA-Seq Expression	Moc08g42100
Synteny	Moc08g42100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGTTGCATAAGTCTCTTGCCATCTTGAGCTCCCTTTGCCTGATCTTCATGGCCATATCTTCATCCACAGCTCAACCTCCTCCGGTGCTCGACACCAACGGCCAAGCTCTACGACGCGGTATTGAGTACTACATCAAGCCCGCGATCACCGACGTTGCAGGCAATCTCACCCTGAAAAGTCGTAGCCGTGCCCCTTGCCCGCTCTACGTTGGCCAGGAACCGGTTGCTTCGATGGAGATCGGTCTCCCAGTCATCTTCACCCCCTACGCCGCGGACGAGGATATAATAAGAGAAGGCATGGATTTCAAGGTTGTGTTCCAGGCGTCGTCGACGTGCATAATGTTGACACAATGGAGAGTGGAGGAGACAGAGGCTGCCACAGGGAGGAGGCTGGTGGGGACCGGGGACGACAACGGCCCGACCGGGATGTTCAGGATCGACAGGAGCAACCTCGGAGTTTACAATATAGGATGGTGTCCCGAGATGTTTGGGAGACCAAGGTGTGGAAGTGCAGGCATTTTGGTTGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

mRNA sequence

ATGACGTTGCATAAGTCTCTTGCCATCTTGAGCTCCCTTTGCCTGATCTTCATGGCCATATCTTCATCCACAGCTCAACCTCCTCCGGTGCTCGACACCAACGGCCAAGCTCTACGACGCGGTATTGAGTACTACATCAAGCCCGCGATCACCGACGTTGCAGGCAATCTCACCCTGAAAAGTCGTAGCCGTGCCCCTTGCCCGCTCTACGTTGGCCAGGAACCGGTTGCTTCGATGGAGATCGGTCTCCCAGTCATCTTCACCCCCTACGCCGCGGACGAGGATATAATAAGAGAAGGCATGGATTTCAAGGTTGTGTTCCAGGCGTCGTCGACGTGCATAATGTTGACACAATGGAGAGTGGAGGAGACAGAGGCTGCCACAGGGAGGAGGCTGGTGGGGACCGGGGACGACAACGGCCCGACCGGGATGTTCAGGATCGACAGGAGCAACCTCGGAGTTTACAATATAGGATGGTGTCCCGAGATGTTTGGGAGACCAAGGTGTGGAAGTGCAGGCATTTTGGTTGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Coding sequence (CDS)

ATGACGTTGCATAAGTCTCTTGCCATCTTGAGCTCCCTTTGCCTGATCTTCATGGCCATATCTTCATCCACAGCTCAACCTCCTCCGGTGCTCGACACCAACGGCCAAGCTCTACGACGCGGTATTGAGTACTACATCAAGCCCGCGATCACCGACGTTGCAGGCAATCTCACCCTGAAAAGTCGTAGCCGTGCCCCTTGCCCGCTCTACGTTGGCCAGGAACCGGTTGCTTCGATGGAGATCGGTCTCCCAGTCATCTTCACCCCCTACGCCGCGGACGAGGATATAATAAGAGAAGGCATGGATTTCAAGGTTGTGTTCCAGGCGTCGTCGACGTGCATAATGTTGACACAATGGAGAGTGGAGGAGACAGAGGCTGCCACAGGGAGGAGGCTGGTGGGGACCGGGGACGACAACGGCCCGACCGGGATGTTCAGGATCGACAGGAGCAACCTCGGAGTTTACAATATAGGATGGTGTCCCGAGATGTTTGGGAGACCAAGGTGTGGAAGTGCAGGCATTTTGGTTGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Protein sequence

MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGSAFPFEFVKA

Homology

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BLAST of Moc08g42100 vs. NCBI nr
Match: XP_022150789.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 399.4 bits (1025), Expect = 1.8e-107
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK 60
           MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK
Sbjct: 1   MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK 60

Query: 61  SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR 120
           SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR
Sbjct: 61  SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR 120

Query: 121 VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV 180
           VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV
Sbjct: 121 VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV 180

Query: 181 RLLALDGSAFPFEFVKA 198
           RLLALDGSAFPFEFVKA
Sbjct: 181 RLLALDGSAFPFEFVKA 197

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BLAST of Moc08g42100 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 310.8 bits (795), Expect = 8.2e-81
Identity = 158/195 (81.03%), Postives = 169/195 (86.67%), Query Frame = 0


Query: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62
           LH SLAI S L L+FMAI +STAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSR
Sbjct: 2   LHNSLAIFSFL-LVFMAI-TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSR 61

Query: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122
           S APCPLYVGQEP+AS  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+
Sbjct: 62  SNAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVD 121

Query: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182
           E E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRL
Sbjct: 122 EMESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRL 181

Query: 183 LALDGSAFPFEFVKA 198
           LALDG+AFPFEFVKA
Sbjct: 182 LALDGNAFPFEFVKA 193

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BLAST of Moc08g42100 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.8 bits (782), Expect = 2.6e-79
Identity = 156/195 (80.00%), Postives = 167/195 (85.64%), Query Frame = 0


Query: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62
           LH SLAI S L L+FMAI +STAQ PPVLDT+GQ L+RG+EYYIKPAITDVAGNLTLKSR
Sbjct: 2   LHNSLAIFSYL-LVFMAI-TSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSR 61

Query: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122
           S APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+
Sbjct: 62  SNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVD 121

Query: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182
           E E+ATGRR VG G+DN PTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRL
Sbjct: 122 EMESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRL 181

Query: 183 LALDGSAFPFEFVKA 198
           LALDG+AFPFEFVKA
Sbjct: 182 LALDGNAFPFEFVKA 193

[Close]

BLAST of Moc08g42100 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 305.4 bits (781), Expect = 3.4e-79
Identity = 155/195 (79.49%), Postives = 168/195 (86.15%), Query Frame = 0


Query: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62
           LH SLAI S L L+F+AI +STAQ PPVLDT+GQ LRRG+EYYIKPAIT+VAGNLTLK+R
Sbjct: 2   LHNSLAIFSYL-LVFLAI-TSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNR 61

Query: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122
           S APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+
Sbjct: 62  SNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVD 121

Query: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182
           E E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRL
Sbjct: 122 EMESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRL 181

Query: 183 LALDGSAFPFEFVKA 198
           LALDG+AFPFEFVKA
Sbjct: 182 LALDGNAFPFEFVKA 193

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BLAST of Moc08g42100 vs. NCBI nr
Match: KAG7030262.1 (hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 299.3 bits (765), Expect = 2.5e-77
Identity = 148/180 (82.22%), Postives = 158/180 (87.78%), Query Frame = 0


Query: 18  MAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCPLYVGQEPVA 77
           MAI +STAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSRS APCPLYVGQEP+A
Sbjct: 1   MAI-TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIA 60

Query: 78  SMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAATGRRLVGTGD 137
           S  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+E E+ATGRR VG G+
Sbjct: 61  STNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGN 120

Query: 138 DNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGSAFPFEFVKA 197
           DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLALDG+AFPFEFVKA
Sbjct: 121 DNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 178

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BLAST of Moc08g42100 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.4e-29
Identity = 81/202 (40.10%), Postives = 116/202 (57.43%), Query Frame = 0


Query: 5   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 64
           ++L IL+ +CL      ++T     VLDT G+ +    EY+I+P IT+  G  T+ SR+ 
Sbjct: 8   RTLIILAHVCLFI----TTTTIAQFVLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSRNE 67

Query: 65  APCPLYVGQEPVASMEIGLPVIFTPYAA--DEDIIREGMDFKVVFQASSTCIMLTQWRVE 124
           + CPL+VG E +  +  GL V FTP+A   D D +R   D ++ FQASS+C+  T+WR+ 
Sbjct: 68  S-CPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLG 127

Query: 125 ETEAATGRRLVGTGDDNGPTG----MFRIDRSNL-GVYNIGWCPEMF---GRPRCGSAGI 184
           E +  +GRRL+ TG D+   G     FRI  + L G+YNI WCP       +  CG+  +
Sbjct: 128 EKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDM 187

Query: 185 LVEDGVRLLALDGSAFPFEFVK 197
           L E+G  LLALDG   P  F K
Sbjct: 188 LNENGKILLALDGGPLPLVFQK 203

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BLAST of Moc08g42100 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.4e-29
Identity = 80/205 (39.02%), Postives = 125/205 (60.98%), Query Frame = 0


Query: 2   TLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKS 61
           T+  ++ IL+++ L  + +++S AQ   V+DT+G+ +    +Y+I+PAIT   G+LTL +
Sbjct: 3   TISFTIFILANVWL--LVVTTSIAQ--FVIDTSGEPVENDEDYFIRPAITGNGGSLTLVT 62

Query: 62  RSRAPCPLYVGQEPVASMEIGLPVIFTPYAA--DEDIIREGMDFKVVFQASSTCIMLTQW 121
           R+   CP  VG +P A    G  V+ +P+ +  +ED +R G D +V+FQA ++C   T+W
Sbjct: 63  RN--SCPFNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEW 122

Query: 122 RVEETEAATGRRLVGTGDDNGPTG----MFRIDRS-NLGVYNIGWCPEMF---GRPRCGS 181
           R+ E +A TGRR + TG D+   G     FRI ++ + G++NI WCP       +  CG+
Sbjct: 123 RLGERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGT 182

Query: 182 AGILVEDGVRLLALDGSAFPFEFVK 197
            GI+ E+G  LLALDGSA P  F K
Sbjct: 183 VGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of Moc08g42100 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.4e-28
Identity = 79/201 (39.30%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 5   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 64
           +SL I     +  +  ++S AQ   V+DT+G+ +    EY+I+PAIT   G  TL +   
Sbjct: 4   RSLTIFILAHVWLLMATTSIAQ--FVIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT-GN 63

Query: 65  APCPLYVGQEPVASMEIGLPVIFTPYAA--DEDIIREGMDFKVVFQASSTCIMLTQWRVE 124
            PCPL+VG +      +G+ V FTP+A   D+D +R   D +V F  S++C   T WR+ 
Sbjct: 64  GPCPLHVGLDNTEG-TLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLG 123

Query: 125 ETEAATGRRLVGTGDDNG---PTGMFRIDRSNL-GVYNIGWCPE---MFGRPRCGSAGIL 184
           E +A +GRRL+ TG DNG       FRI ++   G YNI WCP       + +CG+ G++
Sbjct: 124 EKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVI 183

Query: 185 VEDGVRLLALDGSAFPFEFVK 197
            E+G  LLALDG A P  F K
Sbjct: 184 RENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of Moc08g42100 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-17
Identity = 62/192 (32.29%), Postives = 92/192 (47.92%), Query Frame = 0


Query: 20  ISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKS---RSRAPCPLYVGQEPV 79
           +S++ + P PVLD +G+ LR G  YYI P + D  G LT+ +        CP  V Q   
Sbjct: 26  LSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR- 85

Query: 80  ASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTC--IMLTQWRVEETEAATGRRLVG 139
             ++   P+ F P    ED++R   D  + F A   C     T WR+++ + +TG+  V 
Sbjct: 86  KEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVT 145

Query: 140 TGDDNGPTG------MFRIDR-SNLGVYNIGWCPEMFG--RPRCGSAGILVED-GVRLLA 197
            G   G  G       F+I+     G Y + +CP + G  + +CG  GI ++  G R LA
Sbjct: 146 IGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLA 205

[Close]

BLAST of Moc08g42100 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 8.7e-17
Identity = 58/146 (39.73%), Postives = 78/146 (53.42%), Query Frame = 0


Query: 11  SSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCPLY 70
           SS  L + A  S  A PPPV DT+G  LR    YY+  A     G LT+       CPL+
Sbjct: 9   SSSPLFWPAPPSRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLF 68

Query: 71  VGQEPVASMEIGLPVIFTPY--AADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAAT 130
           V Q+P    + G PV  TPY  A  + IIR   D ++ F+A +TC+  T+W + ++E A 
Sbjct: 69  VSQDPNGQHD-GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAA 128

Query: 131 GRRLVGTG--DDNGPTG---MFRIDR 150
           GRR V TG   D  P+G    FRI++
Sbjct: 129 GRRHVITGPVKDPSPSGRENAFRIEK 152

[Close]

BLAST of Moc08g42100 vs. ExPASy TrEMBL
Match: A0A6J1DB43 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 8.5e-108
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK 60
           MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK
Sbjct: 1   MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK 60

Query: 61  SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR 120
           SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR
Sbjct: 61  SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR 120

Query: 121 VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV 180
           VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV
Sbjct: 121 VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV 180

Query: 181 RLLALDGSAFPFEFVKA 198
           RLLALDGSAFPFEFVKA
Sbjct: 181 RLLALDGSAFPFEFVKA 197

[Close]

BLAST of Moc08g42100 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.0e-81
Identity = 158/195 (81.03%), Postives = 169/195 (86.67%), Query Frame = 0


Query: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62
           LH SLAI S L L+FMAI +STAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSR
Sbjct: 2   LHNSLAIFSFL-LVFMAI-TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSR 61

Query: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122
           S APCPLYVGQEP+AS  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+
Sbjct: 62  SNAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVD 121

Query: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182
           E E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRL
Sbjct: 122 EMESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRL 181

Query: 183 LALDGSAFPFEFVKA 198
           LALDG+AFPFEFVKA
Sbjct: 182 LALDGNAFPFEFVKA 193

[Close]

BLAST of Moc08g42100 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.7e-79
Identity = 155/195 (79.49%), Postives = 168/195 (86.15%), Query Frame = 0


Query: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62
           LH SLAI S L L+F+AI +STAQ PPVLDT+GQ LRRG+EYYIKPAIT+VAGNLTLK+R
Sbjct: 2   LHNSLAIFSYL-LVFLAI-TSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNR 61

Query: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122
           S APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+
Sbjct: 62  SNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVD 121

Query: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182
           E E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRL
Sbjct: 122 EMESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRL 181

Query: 183 LALDGSAFPFEFVKA 198
           LALDG+AFPFEFVKA
Sbjct: 182 LALDGNAFPFEFVKA 193

[Close]

BLAST of Moc08g42100 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.8e-70
Identity = 139/197 (70.56%), Postives = 153/197 (77.66%), Query Frame = 0


Query: 1   MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK 60
           M LHKSLAI    CL FMAI +STAQ PPVLDTNGQ LRRG+EYYI PAITDV GNLTLK
Sbjct: 2   MMLHKSLAIFGYACL-FMAI-TSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLK 61

Query: 61  SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR 120
           SRS APCPL+VGQEPV S  IGLPV F P  A +DII EG    +VF+A STC   TQWR
Sbjct: 62  SRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWR 121

Query: 121 VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV 180
           V+ TE+ TGRR VG GD++GP G+F I R N G YNI WCP M GRPRCG AGILVE+GV
Sbjct: 122 VDATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPAMMGRPRCGRAGILVENGV 181

Query: 181 RLLALDGSAFPFEFVKA 198
           RL+ALDG AFPFEF+KA
Sbjct: 182 RLVALDGDAFPFEFIKA 195

[Close]

BLAST of Moc08g42100 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.1e-70
Identity = 138/195 (70.77%), Postives = 152/195 (77.95%), Query Frame = 0


Query: 3   LHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSR 62
           LHKSLAI    CL FMAI +STAQ PPVLDTNGQ LRRG+EYYI PAITDV GNLTLKSR
Sbjct: 2   LHKSLAIFGYACL-FMAI-TSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSR 61

Query: 63  SRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVE 122
           S APCPL+VGQEPV S  IGLPV F P  A +DII EG    +VF+A STC   TQWRV+
Sbjct: 62  SNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVD 121

Query: 123 ETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRL 182
            TE+ TGRR VG GD++GP G+F I R N G YNI WCP M GRPRCG AGILVE+GVRL
Sbjct: 122 ATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPAMMGRPRCGRAGILVENGVRL 181

Query: 183 LALDGSAFPFEFVKA 198
           +ALDG AFPFEF+KA
Sbjct: 182 VALDGDAFPFEFIKA 193

The following BLAST results are available for this feature:

BLAST of Moc08g42100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150789.1	1.8e-107	100.00	miraculin-like [Momordica charantia]	[more]
XP_022946294.1	8.2e-81	81.03	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_023545477.1	2.6e-79	80.00	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022999136.1	3.4e-79	79.49	miraculin-like [Cucurbita maxima]	[more]
KAG7030262.1	2.5e-77	82.22	hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Moc08g42100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV1	3.4e-29	40.10	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
G7LCV7	3.4e-29	39.02	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	8.4e-28	39.30	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	1.0e-17	32.29	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P07596	8.7e-17	39.73	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of Moc08g42100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DB43	8.5e-108	100.00	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1	[more]
A0A6J1G3G3	4.0e-81	81.03	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	1.7e-79	79.49	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A5D3D468	1.8e-70	70.56	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	4.1e-70	70.77	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]

BLAST of Moc08g42100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 29..197 e-value: 6.0E-27 score: 105.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 30..196 e-value: 1.7E-38 score: 132.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..196
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 30..46
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 29..196 e-value: 3.47695E-37 score: 125.147
None	No IPR available	GENE3D	2.80.10.50		coord: 24..197 e-value: 4.4E-43 score: 148.9
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 8..196
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g42100.1	Moc08g42100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0050896	response to stimulus
molecular_function	GO:0004866	endopeptidase inhibitor activity