Moc08g41050 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g41050
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectinesterase
Location	chr8: 31499400 .. 31500962 (+)
RNA-Seq Expression	Moc08g41050
Synteny	Moc08g41050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCTTCTCCCTCCATTTCTCTTACTCTTCACCCTGTCTAATGCTGCTGTTTTTGGTTCTGAAGCTGTGGCTTATGCAAGCAACTCTGAGGTTTTAAGAATTGAATCCTCCATTGTGGTTGACAAGTCTGGCACGGGCGATTTCTGGACCGTTCAAGCAGCCATCGATTCGGTTCCATCTAATAACAACAAATGGATTAAGATTCAAATCAATCCTGGCGAATACAAGTAATAATGCTAAAACATGTGTATGCCTACTCTATGCTTCCAGCTGCTGTTTCCATTTGCTATTGAGCTTTATTTCTAATTATGGTTGGCAAACATTTTGCAGCGAAAAAGTGACAATCCCAGGGGACAAACCTTACATTTTTCTCGATGGAGGTGATCGTAGGACTACGATTATCACGTTCGATGACCATCAAAGAACAGATCTTAGTGCTACATTTACTTCAATTCCTGATAACGTTGTTGCCAGAGGCATTACCTTTAAGGTGCTGTTGCTTCCATTGCTTGATTTGACATAAACTGAATTTATGTTCACATCATTGAAAATTCATAGCAAATTAGGCTGCAAGTTAATCATTGCTCAATCATTGATCAGTGTCTATCTTGTTAGAATTGTTGCTTGTGTTTGATTAGATATTGTCGTGGTGCAATGCAGAACTCCTTCAATCTTGCAGAAGCGCCGCAACTGTATGATTATAGTGGTGGAACTTACATTACACAAGCAGTAGCTGCAAGAATTTTCGGTGACAGATCGGCATTTTTCGACTGCGGATTCATGGGGTTTCAAGACACATTATGGGATGTACAAGGCCGCCATCTCTTTGAAAATTGCTATATCGAAGGTGCCATAGACTTCATCTTTGGTAGAGGGCAATCAATTTACAAGGTACTCTATAAACTTTCCAAGTTGATGCAAGAAGGCCATTCATGGTTCTGTTTCTGCCAACTTCAAATGCCGATACCGTTTCTTTATTCAATTTGGGGAGACTTACACTGCAACTTGAAACCAAAAATGCATGTTTTTGACAATGCAGGACTGCTTGATAAATGTTAATGTTGCATCTCTCCCTCAAGTGCACCAAGGATTCATTACCGCACAAGGTCGAGAATCGGCAGCAGACTCGAGCGGGTTTATATTTAAACAGTGCAATGTAACGGGGGCTGGCCAAGTGTTTTTGGGGAGAGCTTATCGTCCTTATTCCAGAGTTATATTTGAGGATACTTCATTGGATTCGGTGGTAGCACCAGAGGGATGGGATGCCTGGAACTACAAAGGCCAAGAGTAAGTTCACAGAAACAAAAAGAAACAAAGAAAAGAAATAGCCTCTGACTTTTAAAGTTTTCTTGATTGAATTGCCAAGTTCTTATAATTGTTTTTATTGATGTTACATTCTTTAGGGAAAACTTTATGTATGTTGAGGTGAATGGTAGAGGGCCAGGATCTTCCACTACCAAGAGAGTTCCATGGGAGAAGAAGCTTGATGCCTCAGAACTAAGGAACTTCTCATTGGAATTCTTCATTGACCAAGATGGGTGGATTCCCACGATCCCTTGA

mRNA sequence

ATGCAGCTTCTCCCTCCATTTCTCTTACTCTTCACCCTGTCTAATGCTGCTGTTTTTGGTTCTGAAGCTGTGGCTTATGCAAGCAACTCTGAGGTTTTAAGAATTGAATCCTCCATTGTGGTTGACAAGTCTGGCACGGGCGATTTCTGGACCGTTCAAGCAGCCATCGATTCGGTTCCATCTAATAACAACAAATGGATTAAGATTCAAATCAATCCTGGCGAATACAACGAAAAAGTGACAATCCCAGGGGACAAACCTTACATTTTTCTCGATGGAGGTGATCGTAGGACTACGATTATCACGTTCGATGACCATCAAAGAACAGATCTTAGTGCTACATTTACTTCAATTCCTGATAACGTTGTTGCCAGAGGCATTACCTTTAAGAACTCCTTCAATCTTGCAGAAGCGCCGCAACTGTATGATTATAGTGGTGGAACTTACATTACACAAGCAGTAGCTGCAAGAATTTTCGGTGACAGATCGGCATTTTTCGACTGCGGATTCATGGGGTTTCAAGACACATTATGGGATGTACAAGGCCGCCATCTCTTTGAAAATTGCTATATCGAAGGTGCCATAGACTTCATCTTTGGTAGAGGGCAATCAATTTACAAGGACTGCTTGATAAATGTTAATGTTGCATCTCTCCCTCAAGTGCACCAAGGATTCATTACCGCACAAGGTCGAGAATCGGCAGCAGACTCGAGCGGGTTTATATTTAAACAGTGCAATGTAACGGGGGCTGGCCAAGTGTTTTTGGGGAGAGCTTATCGTCCTTATTCCAGAGTTATATTTGAGGATACTTCATTGGATTCGGTGGTAGCACCAGAGGGATGGGATGCCTGGAACTACAAAGGCCAAGAGGAAAACTTTATGTATGTTGAGGTGAATGGTAGAGGGCCAGGATCTTCCACTACCAAGAGAGTTCCATGGGAGAAGAAGCTTGATGCCTCAGAACTAAGGAACTTCTCATTGGAATTCTTCATTGACCAAGATGGGTGGATTCCCACGATCCCTTGA

Coding sequence (CDS)

ATGCAGCTTCTCCCTCCATTTCTCTTACTCTTCACCCTGTCTAATGCTGCTGTTTTTGGTTCTGAAGCTGTGGCTTATGCAAGCAACTCTGAGGTTTTAAGAATTGAATCCTCCATTGTGGTTGACAAGTCTGGCACGGGCGATTTCTGGACCGTTCAAGCAGCCATCGATTCGGTTCCATCTAATAACAACAAATGGATTAAGATTCAAATCAATCCTGGCGAATACAACGAAAAAGTGACAATCCCAGGGGACAAACCTTACATTTTTCTCGATGGAGGTGATCGTAGGACTACGATTATCACGTTCGATGACCATCAAAGAACAGATCTTAGTGCTACATTTACTTCAATTCCTGATAACGTTGTTGCCAGAGGCATTACCTTTAAGAACTCCTTCAATCTTGCAGAAGCGCCGCAACTGTATGATTATAGTGGTGGAACTTACATTACACAAGCAGTAGCTGCAAGAATTTTCGGTGACAGATCGGCATTTTTCGACTGCGGATTCATGGGGTTTCAAGACACATTATGGGATGTACAAGGCCGCCATCTCTTTGAAAATTGCTATATCGAAGGTGCCATAGACTTCATCTTTGGTAGAGGGCAATCAATTTACAAGGACTGCTTGATAAATGTTAATGTTGCATCTCTCCCTCAAGTGCACCAAGGATTCATTACCGCACAAGGTCGAGAATCGGCAGCAGACTCGAGCGGGTTTATATTTAAACAGTGCAATGTAACGGGGGCTGGCCAAGTGTTTTTGGGGAGAGCTTATCGTCCTTATTCCAGAGTTATATTTGAGGATACTTCATTGGATTCGGTGGTAGCACCAGAGGGATGGGATGCCTGGAACTACAAAGGCCAAGAGGAAAACTTTATGTATGTTGAGGTGAATGGTAGAGGGCCAGGATCTTCCACTACCAAGAGAGTTCCATGGGAGAAGAAGCTTGATGCCTCAGAACTAAGGAACTTCTCATTGGAATTCTTCATTGACCAAGATGGGTGGATTCCCACGATCCCTTGA

Protein sequence

MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTIP

Homology

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BLAST of Moc08g41050 vs. NCBI nr
Match: KAA0048077.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 530.8 bits (1366), Expect = 8.7e-147
Identity = 250/341 (73.31%), Postives = 289/341 (84.75%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQL PPFLLLFTL NA VFG EAV +A   + L ++S +VVDKSG GDF TVQAAIDSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NNN+WIKIQINPG Y EKVTIP +KP+I+L+G DR  TIITFDDHQ+TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ARGITF+NSFNL EAP+L+    GTYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQGFITAQGR SAAD SGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK C +TG+G+  LGRAY P+SRVIF+D  +  VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTIP 342
            GPG+ST+ RVPW K LDAS+LR FS+E FI+QDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Moc08g41050 vs. NCBI nr
Match: TYJ96459.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 530.0 bits (1364), Expect = 1.5e-146
Identity = 250/341 (73.31%), Postives = 289/341 (84.75%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQL PPFLLLFTL NA VFG EAV +A   + L ++S +VVDKSG GDF TVQAAIDSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NNN+WIKIQINPG Y EKVTIP +KP+I+L+G DR  TIITFDDHQ+TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ARGITF+NSFNL EAP+L+    GTYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQGFITAQGR SAAD SGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK C +TG+G+  LGRAY P+SRVIF+D  +  VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTIP 342
            GPG+ST+ RVPW K LDAS+LR FS+E FI+QDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

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BLAST of Moc08g41050 vs. NCBI nr
Match: XP_016901459.1 (PREDICTED: probable pectinesterase 66 [Cucumis melo])

HSP 1 Score: 513.1 bits (1320), Expect = 1.9e-141
Identity = 251/371 (67.65%), Postives = 290/371 (78.17%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQL PPFLLLFTL NA VFG EAV +A   + L ++S +VVDKSG GDF TVQAAIDSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYN------EKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSAT 120
            NNN+WIKIQINPG YN      EKVTIP +KP+I+L+G DR  TIITFDDHQ+TD SAT
Sbjct: 61  PNNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSAT 120

Query: 121 FTSIPDNVVARGITF------------------------KNSFNLAEAPQLYDYSGGTYI 180
           FTS P N++ARGITF                        +NSFNL EAP+L+    GTYI
Sbjct: 121 FTSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYI 180

Query: 181 TQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCL 240
           TQA+AARI+GD+SAFF+CGF G+QDTLWDVQGRH F+NCYIEGAIDFIFG GQS+YKDCL
Sbjct: 181 TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCL 240

Query: 241 INVNVASLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDT 300
           INVNVASLPQVHQGFITAQGR SAAD SGF+FK C +TG+G+  LGRAY P+SRVIF+D 
Sbjct: 241 INVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDA 300

Query: 301 SLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFF 342
            +  VVAPEGWDAWN+K +E+NFMYVE N  GPG+ST+ RVPW K LDAS+LR FS+E F
Sbjct: 301 IMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESF 360

[Close]

BLAST of Moc08g41050 vs. NCBI nr
Match: KGN61008.1 (hypothetical protein Csa_021278 [Cucumis sativus])

HSP 1 Score: 510.4 bits (1313), Expect = 1.2e-140
Identity = 239/340 (70.29%), Postives = 284/340 (83.53%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQ+ PPFLLLFTL NA VFG EAV  A +  VL I+S ++VDKSG G+F TVQAAIDSVP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDF-VLTIQSVVIVDKSGNGNFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NNN WIKIQINPG Y EKVTIP +KP+I+L+G D   T+ITFDDHQ+TD SATFTS P 
Sbjct: 61  PNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ RGITF+NSFNL EAP+L+    GTYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           QGRH F +CYIEGAIDFIFG GQS+Y+DC+INVNVASLPQV+QG+ITAQ R+SAAD SGF
Sbjct: 181 QGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK+C + G+G+  LGRAY P+SRVIF+D  + SVVAPEGW AW++KG+EENFMYVE N 
Sbjct: 241 VFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENC 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTI 341
            GPG+ST+ RVPW K LDAS L  FS+E FI+QDGWIPT+
Sbjct: 301 TGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTV 339

[Close]

BLAST of Moc08g41050 vs. NCBI nr
Match: XP_038889344.1 (probable pectinesterase 66 [Benincasa hispida])

HSP 1 Score: 500.7 bits (1288), Expect = 9.7e-138
Identity = 234/341 (68.62%), Postives = 284/341 (83.28%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           M LL PFLLL TL NA VFG EAV +A   +++ ++S +VVDKSG G+  TVQ+AIDSVP
Sbjct: 1   MLLLAPFLLLVTLFNATVFGFEAVNHARTFDLI-VQSVVVVDKSGRGNVHTVQSAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NN +WIKIQIN G Y EKVTIP +KP+I+L+G  R  T+ITF+DHQ+TD SATFTS P 
Sbjct: 61  PNNIQWIKIQINSGVYKEKVTIPQEKPFIYLEGAGRSNTVITFNDHQQTDTSATFTSYPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ARGITF+NSFNLAEAP+++  + GT+ TQA+AARI+GD+SAFF CGF GFQDTLWDV
Sbjct: 121 NIIARGITFENSFNLAEAPEIFVPNDGTFFTQALAARIYGDKSAFFSCGFKGFQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           +GRH F  CYIEGAIDFIFG GQS+YK+CLINVNVA LPQV+QGFITAQGR+SA D SGF
Sbjct: 181 RGRHFFHACYIEGAIDFIFGSGQSVYKNCLINVNVAKLPQVNQGFITAQGRKSATDPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK+C +TG+G+  LGRAY P+SRVIFED  + +VVAP+GWDAWN+KGQE+NFMYVE N 
Sbjct: 241 VFKRCTITGSGKALLGRAYGPFSRVIFEDAIMGTVVAPDGWDAWNFKGQEQNFMYVEENS 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTIP 342
            GPGSST+ RVPW + LDAS+LR FS+E FI+QD WIPT+P
Sbjct: 301 TGPGSSTSMRVPWARTLDASQLRRFSVESFINQDRWIPTLP 340

[Close]

BLAST of Moc08g41050 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-79
Identity = 147/317 (46.37%), Postives = 197/317 (62.15%), Query Frame = 0


Query: 26  YASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGD 85
           Y  ++   +I  +I VD +G G+F TVQ+AIDS+   N+ WI++    G Y EKVTIP +
Sbjct: 22  YGYSAAAEQIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKE 81

Query: 86  KPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYS 145
           K +I+L G     T+I +DDHQ TD+SATFT+  D++V  GITFKN++N+         +
Sbjct: 82  KGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVP-------N 141

Query: 146 GGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSI 205
               I  AVAAR+ GDR    D  F+G QDTL+D +GRH ++ C I G IDFIFG GQS+
Sbjct: 142 NKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSL 201

Query: 206 YKDCLINVNVA-SLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSR 265
           +K+C +N+ +    P    G ITA  R S +D  GF+F  C VTG G+  LGRA+   +R
Sbjct: 202 FKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNAR 261

Query: 266 VIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRN 325
           VIF+ + L  VV P GWDAW  KG E +  +VE    G G+ T++RVPW KKL  SE+  
Sbjct: 262 VIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDG 321

Query: 326 FSLEFFIDQDGWIPTIP 342
           F+   FIDQDGWI   P
Sbjct: 322 FASVSFIDQDGWISRFP 331

[Close]

BLAST of Moc08g41050 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.7e-79
Identity = 144/306 (47.06%), Postives = 190/306 (62.09%), Query Frame = 0


Query: 36  ESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGD 95
           +  + VD+SG G+F T+Q AIDSVP NN  W  I +  G Y EK+ IP +KP+I L G  
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 96  RRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVA 155
           +R T + +DDH     S TF+++ DN V + ITF NS+N     ++         T AVA
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 156 ARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNV 215
           A I GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFIFG GQSIY+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 216 ASLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSV 275
             L     G+ITAQGR +  D++GFIF  C V G G  FLGR +R YSRVIF +++L  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 276 VAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDG 335
           V PEGWDAWN+ G E   ++ E    G G++  +RV W KKL  S ++N +   FI++ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 330

Query: 336 WIPTIP 342
           W+  +P
Sbjct: 331 WVEDLP 332

[Close]

BLAST of Moc08g41050 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.1e-78
Identity = 143/303 (47.19%), Postives = 192/303 (63.37%), Query Frame = 0


Query: 39  IVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRT 98
           ++VD+SG G+F T+Q AIDSVP NN  W  I +  G Y EK+TIP  KP+I + G  +R+
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 99  TIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARI 158
           T + +DDH     S TF ++ DN V + ITF NS+N     ++           AVAA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 159 FGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASL 218
            GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFI G GQSIY+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 219 PQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAP 278
                G+ITAQGR +A D++GF+F  C V G G+ +LGRA+RPYSRVIF +++L  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 279 EGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIP 338
            GW  WNY+G E+   Y E    G GS+T++R  W KKL AS +++ +   FI++ GW+ 
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 327

Query: 339 TIP 342
            +P
Sbjct: 329 DLP 327

[Close]

BLAST of Moc08g41050 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.4e-77
Identity = 143/309 (46.28%), Postives = 190/309 (61.49%), Query Frame = 0


Query: 34  RIESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDG 93
           +I  +I+V+ +    F TVQ+AIDS+P  N  WI+I I+ G Y+EKVTIP  K YI++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 94  GDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQA 153
           G    TII + DHQ T+ SATFTS P N++  GITFKN +N+A        S  +    A
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA--------SSSSPTKPA 155

Query: 154 VAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINV 213
           VAA + GD+ A  D  F GFQDTL+D  GRH ++ C I G IDFIFG  QSI++ C + +
Sbjct: 156 VAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKL 215

Query: 214 NVASLPQVH-QGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSL 273
            V   P     G ITAQGR+S  D  GF+FK C V G+G+  LGRA++ YSRVIF  +  
Sbjct: 216 RVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 275

Query: 274 DSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFID 333
              + P GWDAW  KGQE +  +VE    G G+ T+KRVPW  K    ++  F+   FID
Sbjct: 276 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 335

Query: 334 QDGWIPTIP 342
           ++GW+  +P
Sbjct: 336 EEGWLSRLP 336

[Close]

BLAST of Moc08g41050 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 2.9e-68
Identity = 128/252 (50.79%), Postives = 172/252 (68.25%), Query Frame = 0


Query: 93  GGDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTY-IT 152
           G  +R T IT++ H  TD+S+TFTS P ++V R ++  N++N      L   +G ++ I 
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIK 101

Query: 153 QAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLI 212
            AVA  ++GD+SAF++C F+G QDT+WD  GRH F+NCYIEGAIDFIFG GQS+Y+DC I
Sbjct: 102 PAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHI 161

Query: 213 NVNVASL-PQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDT 272
           N    +L  +V  G+ITAQGR S +D SGF+F + +V+G+  V+LGRAY P+SRVIF  T
Sbjct: 162 NATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQT 221

Query: 273 SLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELR-NFSLEF 332
            L SVV PEGW +W+Y G E +F Y EV  +G GS  ++RVPW  KL +   +  FS+  
Sbjct: 222 DLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISN 281

Query: 333 FIDQDGWIPTIP 342
           FIDQD WI  IP
Sbjct: 282 FIDQDQWISNIP 291

[Close]

BLAST of Moc08g41050 vs. ExPASy TrEMBL
Match: A0A5A7U1G9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001480 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.2e-147
Identity = 250/341 (73.31%), Postives = 289/341 (84.75%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQL PPFLLLFTL NA VFG EAV +A   + L ++S +VVDKSG GDF TVQAAIDSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NNN+WIKIQINPG Y EKVTIP +KP+I+L+G DR  TIITFDDHQ+TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ARGITF+NSFNL EAP+L+    GTYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQGFITAQGR SAAD SGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK C +TG+G+  LGRAY P+SRVIF+D  +  VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTIP 342
            GPG+ST+ RVPW K LDAS+LR FS+E FI+QDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Moc08g41050 vs. ExPASy TrEMBL
Match: A0A5D3BBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001020 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.2e-147
Identity = 250/341 (73.31%), Postives = 289/341 (84.75%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQL PPFLLLFTL NA VFG EAV +A   + L ++S +VVDKSG GDF TVQAAIDSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NNN+WIKIQINPG Y EKVTIP +KP+I+L+G DR  TIITFDDHQ+TD SATFTS P 
Sbjct: 61  PNNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ARGITF+NSFNL EAP+L+    GTYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           QGRH F+NCYIEGAIDFIFG GQS+YKDCLINVNVASLPQVHQGFITAQGR SAAD SGF
Sbjct: 181 QGRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK C +TG+G+  LGRAY P+SRVIF+D  +  VVAPEGWDAWN+K +E+NFMYVE N 
Sbjct: 241 VFKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENC 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTIP 342
            GPG+ST+ RVPW K LDAS+LR FS+E FI+QDGWIP +P
Sbjct: 301 TGPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIPRLP 340

[Close]

BLAST of Moc08g41050 vs. ExPASy TrEMBL
Match: A0A1S4DZS3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 9.1e-142
Identity = 251/371 (67.65%), Postives = 290/371 (78.17%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQL PPFLLLFTL NA VFG EAV +A   + L ++S +VVDKSG GDF TVQAAIDSVP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFD-LTVQSVVVVDKSGRGDFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYN------EKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSAT 120
            NNN+WIKIQINPG YN      EKVTIP +KP+I+L+G DR  TIITFDDHQ+TD SAT
Sbjct: 61  PNNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSAT 120

Query: 121 FTSIPDNVVARGITF------------------------KNSFNLAEAPQLYDYSGGTYI 180
           FTS P N++ARGITF                        +NSFNL EAP+L+    GTYI
Sbjct: 121 FTSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYI 180

Query: 181 TQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCL 240
           TQA+AARI+GD+SAFF+CGF G+QDTLWDVQGRH F+NCYIEGAIDFIFG GQS+YKDCL
Sbjct: 181 TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCL 240

Query: 241 INVNVASLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDT 300
           INVNVASLPQVHQGFITAQGR SAAD SGF+FK C +TG+G+  LGRAY P+SRVIF+D 
Sbjct: 241 INVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDA 300

Query: 301 SLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFF 342
            +  VVAPEGWDAWN+K +E+NFMYVE N  GPG+ST+ RVPW K LDAS+LR FS+E F
Sbjct: 301 IMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESF 360

[Close]

BLAST of Moc08g41050 vs. ExPASy TrEMBL
Match: A0A0A0LH00 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 5.9e-141
Identity = 239/340 (70.29%), Postives = 284/340 (83.53%), Query Frame = 0


Query: 1   MQLLPPFLLLFTLSNAAVFGSEAVAYASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVP 60
           MQ+ PPFLLLFTL NA VFG EAV  A +  VL I+S ++VDKSG G+F TVQAAIDSVP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDF-VLTIQSVVIVDKSGNGNFQTVQAAIDSVP 60

Query: 61  SNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPD 120
            NNN WIKIQINPG Y EKVTIP +KP+I+L+G D   T+ITFDDHQ+TD SATFTS P 
Sbjct: 61  PNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPP 120

Query: 121 NVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDV 180
           N++ RGITF+NSFNL EAP+L+    GTYITQA+AARI+GD+SAFF+CGF G+QDTLWDV
Sbjct: 121 NIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDV 180

Query: 181 QGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASLPQVHQGFITAQGRESAADSSGF 240
           QGRH F +CYIEGAIDFIFG GQS+Y+DC+INVNVASLPQV+QG+ITAQ R+SAAD SGF
Sbjct: 181 QGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGF 240

Query: 241 IFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNG 300
           +FK+C + G+G+  LGRAY P+SRVIF+D  + SVVAPEGW AW++KG+EENFMYVE N 
Sbjct: 241 VFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENC 300

Query: 301 RGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIPTI 341
            GPG+ST+ RVPW K LDAS L  FS+E FI+QDGWIPT+
Sbjct: 301 TGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTV 339

[Close]

BLAST of Moc08g41050 vs. ExPASy TrEMBL
Match: A0A6J1G5A0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.7e-124
Identity = 207/306 (67.65%), Postives = 255/306 (83.33%), Query Frame = 0


Query: 33  LRIESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLD 92
           L+I+S IVVDKSG G+F T+QAAIDSVP NNNKW+KIQ+NPGEY E VTIP  K +I+L 
Sbjct: 14  LKIQSVIVVDKSGKGNFKTIQAAIDSVPLNNNKWVKIQVNPGEYREMVTIPSTKSFIYLQ 73

Query: 93  GGDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQ 152
           GG R TT+ITF+ HQ+T+ SATF S+ DN++ARGITF+NSFNLA APQL+D   GT+ TQ
Sbjct: 74  GGSRSTTVITFNGHQQTNTSATFNSMADNIIARGITFENSFNLANAPQLFDKITGTFFTQ 133

Query: 153 AVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLIN 212
           A+AARI+GD+SAF  CGF GFQDTLWDV+GRH F++CYIEGAIDFIFG GQSIY+ CLI 
Sbjct: 134 ALAARIYGDKSAFISCGFKGFQDTLWDVKGRHYFKSCYIEGAIDFIFGNGQSIYEGCLIY 193

Query: 213 VNVASLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSL 272
           VNV  LP+V+QG+ITAQGR+SA D SGF+F+QC +TG+G+ FLGRAY P+SRVIF+DT++
Sbjct: 194 VNVGLLPEVNQGYITAQGRQSANDPSGFVFQQCTITGSGKGFLGRAYGPFSRVIFKDTNM 253

Query: 273 DSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFID 332
             VVAPEGWDAW++KGQE NF+Y+E N RG GSS +KRVPW      S+L  +S++ FI+
Sbjct: 254 GKVVAPEGWDAWHFKGQETNFVYIESNSRGIGSSPSKRVPWAMTQGNSQLSGYSVKSFIN 313

Query: 333 QDGWIP 339
           QDGWIP
Sbjct: 314 QDGWIP 319

[Close]

BLAST of Moc08g41050 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.2e-80
Identity = 147/317 (46.37%), Postives = 197/317 (62.15%), Query Frame = 0


Query: 26  YASNSEVLRIESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGD 85
           Y  ++   +I  +I VD +G G+F TVQ+AIDS+   N+ WI++    G Y EKVTIP +
Sbjct: 22  YGYSAAAEQIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKE 81

Query: 86  KPYIFLDGGDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYS 145
           K +I+L G     T+I +DDHQ TD+SATFT+  D++V  GITFKN++N+         +
Sbjct: 82  KGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVP-------N 141

Query: 146 GGTYITQAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSI 205
               I  AVAAR+ GDR    D  F+G QDTL+D +GRH ++ C I G IDFIFG GQS+
Sbjct: 142 NKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSL 201

Query: 206 YKDCLINVNVA-SLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSR 265
           +K+C +N+ +    P    G ITA  R S +D  GF+F  C VTG G+  LGRA+   +R
Sbjct: 202 FKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNAR 261

Query: 266 VIFEDTSLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRN 325
           VIF+ + L  VV P GWDAW  KG E +  +VE    G G+ T++RVPW KKL  SE+  
Sbjct: 262 VIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDG 321

Query: 326 FSLEFFIDQDGWIPTIP 342
           F+   FIDQDGWI   P
Sbjct: 322 FASVSFIDQDGWISRFP 331

[Close]

BLAST of Moc08g41050 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.4e-80
Identity = 144/306 (47.06%), Postives = 190/306 (62.09%), Query Frame = 0


Query: 36  ESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGD 95
           +  + VD+SG G+F T+Q AIDSVP NN  W  I +  G Y EK+ IP +KP+I L G  
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 96  RRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVA 155
           +R T + +DDH     S TF+++ DN V + ITF NS+N     ++         T AVA
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 156 ARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNV 215
           A I GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFIFG GQSIY+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 216 ASLPQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSV 275
             L     G+ITAQGR +  D++GFIF  C V G G  FLGR +R YSRVIF +++L  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 276 VAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDG 335
           V PEGWDAWN+ G E   ++ E    G G++  +RV W KKL  S ++N +   FI++ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 330

Query: 336 WIPTIP 342
           W+  +P
Sbjct: 331 WVEDLP 332

[Close]

BLAST of Moc08g41050 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.2e-79
Identity = 143/303 (47.19%), Postives = 192/303 (63.37%), Query Frame = 0


Query: 39  IVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDGGDRRT 98
           ++VD+SG G+F T+Q AIDSVP NN  W  I +  G Y EK+TIP  KP+I + G  +R+
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 99  TIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQAVAARI 158
           T + +DDH     S TF ++ DN V + ITF NS+N     ++           AVAA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 159 FGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINVNVASL 218
            GD+SAF+  GF G QDTLWD  GRH F  C I+GA+DFI G GQSIY+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 219 PQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSLDSVVAP 278
                G+ITAQGR +A D++GF+F  C V G G+ +LGRA+RPYSRVIF +++L  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 279 EGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFIDQDGWIP 338
            GW  WNY+G E+   Y E    G GS+T++R  W KKL AS +++ +   FI++ GW+ 
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 327

Query: 339 TIP 342
            +P
Sbjct: 329 DLP 327

[Close]

BLAST of Moc08g41050 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 3.2e-78
Identity = 143/309 (46.28%), Postives = 190/309 (61.49%), Query Frame = 0


Query: 34  RIESSIVVDKSGTGDFWTVQAAIDSVPSNNNKWIKIQINPGEYNEKVTIPGDKPYIFLDG 93
           +I  +I+V+ +    F TVQ+AIDS+P  N  WI+I I+ G Y+EKVTIP  K YI++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 94  GDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTYITQA 153
           G    TII + DHQ T+ SATFTS P N++  GITFKN +N+A        S  +    A
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA--------SSSSPTKPA 155

Query: 154 VAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLINV 213
           VAA + GD+ A  D  F GFQDTL+D  GRH ++ C I G IDFIFG  QSI++ C + +
Sbjct: 156 VAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKL 215

Query: 214 NVASLPQVH-QGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDTSL 273
            V   P     G ITAQGR+S  D  GF+FK C V G+G+  LGRA++ YSRVIF  +  
Sbjct: 216 RVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 275

Query: 274 DSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELRNFSLEFFID 333
              + P GWDAW  KGQE +  +VE    G G+ T+KRVPW  K    ++  F+   FID
Sbjct: 276 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 335

Query: 334 QDGWIPTIP 342
           ++GW+  +P
Sbjct: 336 EEGWLSRLP 336

[Close]

BLAST of Moc08g41050 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.0e-69
Identity = 128/252 (50.79%), Postives = 172/252 (68.25%), Query Frame = 0


Query: 93  GGDRRTTIITFDDHQRTDLSATFTSIPDNVVARGITFKNSFNLAEAPQLYDYSGGTY-IT 152
           G  +R T IT++ H  TD+S+TFTS P ++V R ++  N++N      L   +G ++ I 
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIK 101

Query: 153 QAVAARIFGDRSAFFDCGFMGFQDTLWDVQGRHLFENCYIEGAIDFIFGRGQSIYKDCLI 212
            AVA  ++GD+SAF++C F+G QDT+WD  GRH F+NCYIEGAIDFIFG GQS+Y+DC I
Sbjct: 102 PAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHI 161

Query: 213 NVNVASL-PQVHQGFITAQGRESAADSSGFIFKQCNVTGAGQVFLGRAYRPYSRVIFEDT 272
           N    +L  +V  G+ITAQGR S +D SGF+F + +V+G+  V+LGRAY P+SRVIF  T
Sbjct: 162 NATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQT 221

Query: 273 SLDSVVAPEGWDAWNYKGQEENFMYVEVNGRGPGSSTTKRVPWEKKLDASELR-NFSLEF 332
            L SVV PEGW +W+Y G E +F Y EV  +G GS  ++RVPW  KL +   +  FS+  
Sbjct: 222 DLSSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISN 281

Query: 333 FIDQDGWIPTIP 342
           FIDQD WI  IP
Sbjct: 282 FIDQDQWISNIP 291

The following BLAST results are available for this feature:

BLAST of Moc08g41050 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0048077.1	8.7e-147	73.31	putative pectinesterase 66 [Cucumis melo var. makuwa]	[more]
TYJ96459.1	1.5e-146	73.31	putative pectinesterase 66 [Cucumis melo var. makuwa]	[more]
XP_016901459.1	1.9e-141	67.65	PREDICTED: probable pectinesterase 66 [Cucumis melo]	[more]
KGN61008.1	1.2e-140	70.29	hypothetical protein Csa_021278 [Cucumis sativus]	[more]
XP_038889344.1	9.7e-138	68.62	probable pectinesterase 66 [Benincasa hispida]	[more]

BLAST of Moc08g41050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4PSQ5	1.6e-79	46.37	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
Q4PSN0	4.7e-79	47.06	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
Q3E9D3	3.1e-78	47.19	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
O64479	4.4e-77	46.28	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
O04953	2.9e-68	50.79	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of Moc08g41050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U1G9	4.2e-147	73.31	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00148...	[more]
A0A5D3BBR9	7.2e-147	73.31	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00102...	[more]
A0A1S4DZS3	9.1e-142	67.65	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1	[more]
A0A0A0LH00	5.9e-141	70.29	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1	[more]
A0A6J1G5A0	1.7e-124	67.65	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1	[more]

BLAST of Moc08g41050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47280.1	1.2e-80	46.37	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	3.4e-80	47.06	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	2.2e-79	47.19	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	3.2e-78	46.28	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	2.0e-69	50.79	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 39..333 e-value: 6.2E-62 score: 209.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 32..340 e-value: 1.1E-101 score: 342.4
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 3..341
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 3..341
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 191..200
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 38..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g41050.1	Moc08g41050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity