Moc08g36860 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g36860
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr8: 27445744 .. 27450821 (+)
RNA-Seq ExpressionMoc08g36860
SyntenyMoc08g36860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAACCTCTGCGCCATACTCTCGCCGCCGCCCAAACCAGCGGCGAAGCAACCGGCGAACACCTTCCCGGACCCGCCGCCTCTGTCCATTTCCAGCAACCGATGGTCTCGAATGCGGTCCTCGCGGAGGGAGAAGCCCGGAGAGTCGCTGACTCCGGAGCAGGCTCTGGAAGTAGCCGCGGCGGCGCTCTACCAGAAACGCCCTCTGGATTCTTCTCTGCCGTTTGATCGGTCTACTTCGCTCCGGCAACCGGCCCCCGGCAAGAAGAATCGGAACGCCTTGCCGCGGAGTTCGAGCTCGCGGCCTCGCTCGCTCACTGACCCTCTTCTTCAGCCTCATCAGCTTGTTAATCAGGTTGGTCGAGATTGTTGGACTTCGAGTTTTGAAATTGATATGAATGATCGGAGATTCGTTGTTTGATTGTTGATGGCTGTTTTTTTTCCTTCTTTATGATGCAAATTGTGAATTTTGTTTGGGGGATTTCTAATTTATCTGTGTGGGAAAACCGAATCATTTTTGGGTCAGTTTTGGAACCAATTTTAGTCAGAAATTCAATCGGATTGATCCCTCTAAGTGCATTTACTTTCACTTTGAATGAGAGCATTTAAACATTCAATTGGATTCCAATCATTCAAAAATATAATGAAATGAATGCTATCTAAAATTTTCTCATCCTGTATTTAATAAACAGTTTCAGTGTAAATACTATATGTCAAATATGGACATTTTAAACTAGAAAGAGAATAAATTAACATAGTTTTGATCAATGGTTCTCAAAATAAAGGAGTAAAGAATGATAATAGTCCCCTGAAAAGGAAAGGAGTAAAACATAATGATGTGTATAATTCCAAAGATTTTAATATAGAATCGGAATCAGTCTGTATTGATGCACGTCTAAATCAAAATAGACCAATTGGATATTCTGTTCAAATTATTAATGGAGATTGCAACTATATTCTGCCATTTATTTTGTCTTTATTCTTATACCATTTTACAAATGGTTATTGTTTGAATTTCTTGGCTAAAGGATTTGTGGTTCTAATAAGTTTCAAATTTACAGGATATAAAGTTAGATGACTTGGAAACAAATCACTTTGTTCTTGTCCATGGAGGTGGTTTTGGTGCTTGGTGTTGGTATAAAACTATTGCCCTTCTTGAAGAAGCGGGCTATAGAGCCACGGCAATAGACCTAACTGGCTCAGGAATTCATTCGTTTGATCCGAACAGCGTCGTGAATCTGGCGCAGTATGTGCAGCCACTTACTGATTTCCTTGAAAAGCTTCCTGATGACGAAAAGGTTTCCTCATTCCCATATCAAACGTCCTTTTTAACTCAGTTTATTAAGTATGTATATATTCATAGATATACGATATGTACATACATTAGTATGTATGTATATTGAATGCGTATTCTCATGGGTGTCGAGAATATACCGAAAATATTAAATTTACTTAAGCCCAATAGTTGGAGACCTTCTCGGGTCTAGGGGTGGTTGAACTATAATGTTCTTGAGACAGTTTCTCTGTTTCAATCGACTTTGTTTGTTTCCAATCACGCTGGTGTATTTTGTGCTTAACTTCCTCTCTCTAAGCCGAATTTCTTTCATATTTTTAATTTATGCAGGTTATCCTAGTTGGGCATGATTTTGGTGGTGCTTGTATTTCATATGAAATGGAATTATTTCCTCTTAAAATTGCAAAGGCTATTTTCATTGCTGCAGCAATGTTGAGTGATGGCCAAAACACTCTTGATATGTTCTCTTTACAGGTATTCTCATCTGCTGATAAATTCCAAATTTTTTGTACCTTTGGACTTGATTATATATATGTTGAAGTTTGAATTCATTTAAAATCAGTATCTGTCGGTATGAATGCTTAGTTTGTGAGAATTCAGATGCTGTTTCTCTTCTCTCTTGTTTCTGGCTTTTAGTCGATGCTGCTCGGCAATCTTTCTGCACTCCTAGGATCTTGTTGATTTTAGTTTCTTTCCAATGAACTATGCTTGATTCTGTGCTAGGCTGGTTCGGACGATGTCATGCGACAGGCTCAAGTCTTCGTGTATGCGAATGGCAACGGCAAGACTCCAACTGCTATTGAATTGAAAAAACCATTGTTAAAGGACTTGTTCTTCAATCAAACTCCAACCAAGGTAACTACGGGCTCGTTTGATAACGTTTTCGTTTCTTGTTTCTTATTTTCAATTTTCTTAAGAAATCAAAGAATTTTACAACTAACTTTGTTTCTTGTTTCTACTTTTTTGGAACATTCTCACAATTGTATAGAAATTTTGGAAACAACACAAACAAGTTTCTATTTTCTTGTTCTCATTTCCAATTTGTTCCAAAACATAAATAAAAATATTGTTTTATCATACTTGAATTTTGAAACAAGAAACAGGAACATTACCAAACGTGCCCTACTTTTCTTCTTTAATTCTTTCACTTTCTTACATAACTCATATAGATTATGATATACCCGAAACAATGAACAAATAGTGCGATCACTAAAACTTAGCCAATTGATCACTCCATTGTCCTTACTCAGGATGTAGCTTTGGCATCTGTTTCAATGAGACCGATACCCTTCCCACCTGTTTTAGAAAAACTCCATCTTTCAGATGCAAAGTACAGATCAGTGAGACGGTTTTACATACAGACGCTGAACGACAATGCCGTACCCGCCCCAATCCAGGCGAGCTTGATCACAAGAAACCCTCCGGAACAGGTCTTCAGTCTAAAAGGTGCAGATCATTCCCCCTTTTTCTCAAAGCCTCAGGCATTGCATAGGCTATTGGTAGAGATCTCAAGAATCAAAAGATCTTAGGAAGCAGTCTCTCTCACCAGCTAGATTGGTTTGCATTGAAAGTTTTTCCAAGCTTTTGCTTTATTGGTATGGCATGTGAATGAATAAAGTTGGTGGGGCTGCTCTCCAAGAGGGATGAAATGAAAAGCCCTGATCTTGCCACCTCATTTTCTATTTCTTCTTCTTATTTTTTTATAAAAATATTACATTAATTAAAAGAGCAATGAGGTTGCAAGATGAGAATCATCTGGTCCCAAAACGGGCTGTTTTAATGGCCTTAATTGGAGGAGAGGTCAGCCCAAAGTTTGGGTATCAAAGTATCAATAATATGAATGCAATTAGGCATTGAAAGAAGTTCGGAATCAAGTTATACTTTCCTCTTTGCTTCTGTTTTTATTTTATTTTTTTAGGGAAAGATAACTACATATTGCATCAAGGTTAAAATACTATTTTGGTCCTGTACTTTCGATTTTGGTTCATTTTGATCTTTGTACCTTTGAATGTTCATTTTGGTCGTTGTAATTTTGTGTTCATATTGGTCATTATACTCTAAATTTTTGTCTATTTTGGTCAATTGTGTTTTCTAAATATTTATTTTGTTATCTACATTTTTTGGTCAATTCATTTTATTTTTTATCTCATTTTTCCGATCACAGTTTTAACACAATACTTCACTTGATATATTTCTTGAAATGTAGTCATAACTTTAAATTAAAGCGTTATGTATAAAATTTGTGTTAAAATTTTACTAATAGAGACCAAAATAGCCACTTTCTAAAAGTTCAATGACTAAAAACATATTTAAATACATAGACTAAGATGAACCTCCGAACAGTGATCAAAATATAGATAAAGCTAAGAATACAGCTACGGAAATAATATTTTAATCTGTATTAAATATGTAAATATTGTCTTTTATTTAATTGTTATTTTCCCCTATTTTCAGAAAAGAAAGAGGGGATTAGAAAAAGAAAGATATTAGAACAACCAACCACTTTTTTTTTTTTTTTTAAGGATAAATACGTAACTAGGTGCGCCTATGTTTAGGTTTGCTCTCTGATAAATTTTTCGTTCTCTGTATTTTAAAATTATGCTTCCAAATGTACGGTTTCTTCGATGTGTTTATCACCTCTCTTCAAAAGGCATTTGAATTCCAAACTAAAATCCAATGACCCAAGTTGGTAAAACTATTTTAGTTTTCATAATTTGAGTTAAGTTGAAAAACTAGATAACAAAACACATAAATACATAGTGTTTATAAGCTTAATTTCCAAAAATAAAAAACAAAAAATAAAATGTTATTTATAAGCTTAATTTCCAAAAATAAAAAACAAAAAATAAAATGTTATCAAATGACACCTTTAAAACACAGTTTTTAAAAACTTGATTTTGTTTCAAAAATTTGATTCAGAAGTCAAACATATTGTGAAGAAAGTTGAAAAATACATGCAAATGCAAAACAAATGGCGAGGAAGCAAAGCAAAGAAATCTGCGTCACAAATGAGTCCAAAGGTATTGAGCTTAACAATGTTGAGTAGTTTAATTTTAAGTGCAAGAAGAAGGGTGGGCAGCATATAACATGTGTTGTGGCCTCAGTAAACATGCAAGAATTATATTGGACCCAACGTTTTTAAAAAATATATCGGGAGAAAATTCACACACCCGCGAGTCAAGCTTTCTCTCGACGTTTTTAAAAAATTGTTGGGATAGGTCTATTTCTCCCGATGATTTTTAAAAATCCGTTGGGAGGAGTTTACTAATATCAAACAACTAGAATAAACTCATAATTCTTGTAGCGTAGGTCGATGCAGATATGAGGGATTTGGGCTTGAAAGATGATACTTGTATTATTGAATTTGATTTTCTGCTGGTTAAAAATTCAGTTTGAAAATAATTTAACTCAACATTTCTCCCTGTCATACTTTAAGTTTGATAGCTTCCCCATTATTTTTGCTAGGTGTAACCAAAGTATTAAATCCAATGGAAGACATTTTCATACGGTCATGAGTGGGACATTTTCGGAAAAATTATATTTCGTGGACTAAATACAATTTATATTTGATATTTGGGGTTCATACAATCTCTAATATTTATCATATGATTCTTTCCCTTGAATTCTTGGTTTTGATCGGCCTCTTGGCGGCTTGTGGGCGGCTTTGTTGATCTTGATCGGTCTATCGGCAGTTTGTTGATCTTGATCGGCCTATTGGCGGCTTGTTGATCTTGATCGGCCTCAAGCTTGGTGATGATCTTGATCGACCTCTGGCTTTGTGA

mRNA sequence

ATGGGGAACCTCTGCGCCATACTCTCGCCGCCGCCCAAACCAGCGGCGAAGCAACCGGCGAACACCTTCCCGGACCCGCCGCCTCTGTCCATTTCCAGCAACCGATGGTCTCGAATGCGGTCCTCGCGGAGGGAGAAGCCCGGAGAGTCGCTGACTCCGGAGCAGGCTCTGGAAGTAGCCGCGGCGGCGCTCTACCAGAAACGCCCTCTGGATTCTTCTCTGCCGTTTGATCGGTCTACTTCGCTCCGGCAACCGGCCCCCGGCAAGAAGAATCGGAACGCCTTGCCGCGGAGTTCGAGCTCGCGGCCTCGCTCGCTCACTGACCCTCTTCTTCAGCCTCATCAGCTTGTTAATCAGGATATAAAGTTAGATGACTTGGAAACAAATCACTTTGTTCTTGTCCATGGAGGTGGTTTTGGTGCTTGGTGTTGGTATAAAACTATTGCCCTTCTTGAAGAAGCGGGCTATAGAGCCACGGCAATAGACCTAACTGGCTCAGGAATTCATTCGTTTGATCCGAACAGCGTCGTGAATCTGGCGCAGTATGTGCAGCCACTTACTGATTTCCTTGAAAAGCTTCCTGATGACGAAAAGGTTATCCTAGTTGGGCATGATTTTGGTGGTGCTTGTATTTCATATGAAATGGAATTATTTCCTCTTAAAATTGCAAAGGCTATTTTCATTGCTGCAGCAATGTTGAGTGATGGCCAAAACACTCTTGATATGTTCTCTTTACAGGCTGGTTCGGACGATGTCATGCGACAGGCTCAAGTCTTCGTGTATGCGAATGGCAACGGCAAGACTCCAACTGCTATTGAATTGAAAAAACCATTGTTAAAGGACTTGTTCTTCAATCAAACTCCAACCAAGGATGTAGCTTTGGCATCTGTTTCAATGAGACCGATACCCTTCCCACCTGTTTTAGAAAAACTCCATCTTTCAGATGCAAAGTACAGATCAGTGAGACGGTTTTACATACAGACGCTGAACGACAATGCCGTACCCGCCCCAATCCAGGCGAGCTTGATCACAAGAAACCCTCCGGAACAGGTCTTCAGTCTAAAAGGTGCAGATCATTCCCCCTTTTTCTCAAAGCCTCAGGCATTGCATAGGCTATTGTTTGTTGATCTTGATCGGCCTATTGGCGGCTTGTTGATCTTGATCGGCCTCAAGCTTGGTGATGATCTTGATCGACCTCTGGCTTTGTGA

Coding sequence (CDS)

ATGGGGAACCTCTGCGCCATACTCTCGCCGCCGCCCAAACCAGCGGCGAAGCAACCGGCGAACACCTTCCCGGACCCGCCGCCTCTGTCCATTTCCAGCAACCGATGGTCTCGAATGCGGTCCTCGCGGAGGGAGAAGCCCGGAGAGTCGCTGACTCCGGAGCAGGCTCTGGAAGTAGCCGCGGCGGCGCTCTACCAGAAACGCCCTCTGGATTCTTCTCTGCCGTTTGATCGGTCTACTTCGCTCCGGCAACCGGCCCCCGGCAAGAAGAATCGGAACGCCTTGCCGCGGAGTTCGAGCTCGCGGCCTCGCTCGCTCACTGACCCTCTTCTTCAGCCTCATCAGCTTGTTAATCAGGATATAAAGTTAGATGACTTGGAAACAAATCACTTTGTTCTTGTCCATGGAGGTGGTTTTGGTGCTTGGTGTTGGTATAAAACTATTGCCCTTCTTGAAGAAGCGGGCTATAGAGCCACGGCAATAGACCTAACTGGCTCAGGAATTCATTCGTTTGATCCGAACAGCGTCGTGAATCTGGCGCAGTATGTGCAGCCACTTACTGATTTCCTTGAAAAGCTTCCTGATGACGAAAAGGTTATCCTAGTTGGGCATGATTTTGGTGGTGCTTGTATTTCATATGAAATGGAATTATTTCCTCTTAAAATTGCAAAGGCTATTTTCATTGCTGCAGCAATGTTGAGTGATGGCCAAAACACTCTTGATATGTTCTCTTTACAGGCTGGTTCGGACGATGTCATGCGACAGGCTCAAGTCTTCGTGTATGCGAATGGCAACGGCAAGACTCCAACTGCTATTGAATTGAAAAAACCATTGTTAAAGGACTTGTTCTTCAATCAAACTCCAACCAAGGATGTAGCTTTGGCATCTGTTTCAATGAGACCGATACCCTTCCCACCTGTTTTAGAAAAACTCCATCTTTCAGATGCAAAGTACAGATCAGTGAGACGGTTTTACATACAGACGCTGAACGACAATGCCGTACCCGCCCCAATCCAGGCGAGCTTGATCACAAGAAACCCTCCGGAACAGGTCTTCAGTCTAAAAGGTGCAGATCATTCCCCCTTTTTCTCAAAGCCTCAGGCATTGCATAGGCTATTGTTTGTTGATCTTGATCGGCCTATTGGCGGCTTGTTGATCTTGATCGGCCTCAAGCTTGGTGATGATCTTGATCGACCTCTGGCTTTGTGA

Protein sequence

MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVAAAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLLFVDLDRPIGGLLILIGLKLGDDLDRPLAL
Homology
BLAST of Moc08g36860 vs. NCBI nr
Match: XP_022158512.1 (putative methylesterase 11, chloroplastic [Momordica charantia])

HSP 1 Score: 747.7 bits (1929), Expect = 5.3e-212
Identity = 375/379 (98.94%), Postives = 377/379 (99.47%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA
Sbjct: 72  MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 131

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD
Sbjct: 132 AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 191

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA
Sbjct: 192 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 251

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL
Sbjct: 252 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 311

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR
Sbjct: 312 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 371

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS
Sbjct: 372 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 431

Query: 361 PFFSKPQALHRLLFVDLDR 380
           PFFSKPQALHRLL V++ R
Sbjct: 432 PFFSKPQALHRLL-VEISR 449

BLAST of Moc08g36860 vs. NCBI nr
Match: KAG6607066.1 (putative methylesterase 11, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 628.2 bits (1619), Expect = 4.7e-176
Identity = 317/373 (84.99%), Postives = 334/373 (89.54%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGN+CA  S PPKP  KQP NTFP+PPPLSISSNRW R RSSRREK GES++ EQAL VA
Sbjct: 1   MGNICATFS-PPKPVVKQPENTFPNPPPLSISSNRWFRTRSSRREKVGESMSSEQAL-VA 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P+D S  FDRSTSLRQP  G+KNRN LPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKHPMDCS--FDRSTSLRQPPSGRKNRNVLPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEE GYRATAIDLTGSG+HSFDPNS+ +L+
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEVGYRATAIDLTGSGVHSFDPNSITDLS 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAMLSDGQNTL
Sbjct: 181 QYVQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHFKIAKAVFIAAAMLSDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAG+DDVMRQAQVFVYANGN   PT IELKKPLLKDLFFNQT  KDVALASVSMR
Sbjct: 241 DMFSLQAGADDVMRQAQVFVYANGNDNAPTTIELKKPLLKDLFFNQTTAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKL LS+ KY SVRRFYIQTLNDNA+P  IQ +LI R+PPEQVF LKGADHS
Sbjct: 301 PIPFPPVLEKLRLSETKYGSVRRFYIQTLNDNAIPVSIQETLIERSPPEQVFILKGADHS 360

Query: 361 PFFSKPQALHRLL 374
           PFFSKPQALHRLL
Sbjct: 361 PFFSKPQALHRLL 369

BLAST of Moc08g36860 vs. NCBI nr
Match: XP_023524731.1 (putative methylesterase 11, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.9 bits (1618), Expect = 6.2e-176
Identity = 317/373 (84.99%), Postives = 334/373 (89.54%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  S PPKP  KQP NTFP+PPPLSISSNRW R RSSRREK GES++ EQAL VA
Sbjct: 1   MGNLCATFS-PPKPVVKQPENTFPNPPPLSISSNRWFRTRSSRREKVGESMSSEQAL-VA 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P+D S  FDRSTSLRQP  G+KNRN LPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKHPMDCS--FDRSTSLRQPPSGRKNRNVLPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEE GYRATAIDLTGSG+HSFDPNS+ +L+
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEVGYRATAIDLTGSGVHSFDPNSITDLS 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAMLSDGQNTL
Sbjct: 181 QYVQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHFKIAKAVFIAAAMLSDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAG+DDVMRQAQVFVYANGN   PT IELKKPLLKDLFFNQT  KDVALASVSMR
Sbjct: 241 DMFSLQAGADDVMRQAQVFVYANGNDNAPTTIELKKPLLKDLFFNQTTAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKL LS+ +Y SVRRFYIQTLNDNA+P  IQ +LI R+PPEQVF LKGADHS
Sbjct: 301 PIPFPPVLEKLRLSETRYGSVRRFYIQTLNDNAIPVSIQETLIERSPPEQVFILKGADHS 360

Query: 361 PFFSKPQALHRLL 374
           PFFSKPQALHRLL
Sbjct: 361 PFFSKPQALHRLL 369

BLAST of Moc08g36860 vs. NCBI nr
Match: XP_022998085.1 (putative methylesterase 11, chloroplastic [Cucurbita maxima])

HSP 1 Score: 627.1 bits (1616), Expect = 1.1e-175
Identity = 317/373 (84.99%), Postives = 333/373 (89.28%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  S PPKP  KQP NTFP+PPPLSISSNRW R RSSRREK GES++ EQAL VA
Sbjct: 1   MGNLCATFS-PPKPVVKQPENTFPNPPPLSISSNRWFRTRSSRREKVGESMSSEQAL-VA 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P+D S  FDRSTSLRQP  G KNRN LPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKHPMDCS--FDRSTSLRQPPSGSKNRNVLPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEE GYRATAIDLTGSG+HSFDPNS+ +L+
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEVGYRATAIDLTGSGVHSFDPNSITDLS 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAMLSDGQNTL
Sbjct: 181 QYVQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHFKIAKAVFIAAAMLSDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMF+LQAG+DDVMRQAQVFVYANGN   PT IELKKPLLKDLFFNQT  KDVALASVSMR
Sbjct: 241 DMFTLQAGADDVMRQAQVFVYANGNDNAPTTIELKKPLLKDLFFNQTTAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKL LS+ KY SVRRFYIQTLNDNA+P  IQ +LI R+PPEQVF LKGADHS
Sbjct: 301 PIPFPPVLEKLRLSETKYGSVRRFYIQTLNDNAIPVSIQETLIERSPPEQVFILKGADHS 360

Query: 361 PFFSKPQALHRLL 374
           PFFSKPQALHRLL
Sbjct: 361 PFFSKPQALHRLL 369

BLAST of Moc08g36860 vs. NCBI nr
Match: XP_022948800.1 (putative methylesterase 11, chloroplastic [Cucurbita moschata])

HSP 1 Score: 626.3 bits (1614), Expect = 1.8e-175
Identity = 316/373 (84.72%), Postives = 333/373 (89.28%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  S PPKP  KQP NTFP+PPPLSISSNRW R RSSRREK GES++ EQAL  A
Sbjct: 1   MGNLCATFS-PPKPVVKQPENTFPNPPPLSISSNRWFRTRSSRREKVGESMSSEQAL-AA 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P+D S  FDRSTSLRQP  G+KNRN LPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKHPMDCS--FDRSTSLRQPPSGRKNRNVLPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEE GYRATAIDLTGSG+HSFDPNS+ +L+
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEVGYRATAIDLTGSGVHSFDPNSITDLS 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAMLSDGQNTL
Sbjct: 181 QYVQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHFKIAKAVFIAAAMLSDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAG+DDVMRQAQVFVYANGN   PT IELKKPLLKDLFFNQT  KDVALASVSMR
Sbjct: 241 DMFSLQAGADDVMRQAQVFVYANGNDNAPTTIELKKPLLKDLFFNQTTAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKL LS+ +Y SVRRFYIQTLNDNA+P  IQ +LI R+PPEQVF LKGADHS
Sbjct: 301 PIPFPPVLEKLRLSETRYGSVRRFYIQTLNDNAIPVSIQETLIERSPPEQVFILKGADHS 360

Query: 361 PFFSKPQALHRLL 374
           PFFSKPQALHRLL
Sbjct: 361 PFFSKPQALHRLL 369

BLAST of Moc08g36860 vs. ExPASy Swiss-Prot
Match: Q9FW03 (Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES11 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 9.7e-132
Identity = 244/382 (63.87%), Postives = 293/382 (76.70%), Query Frame = 0

Query: 1   MGNLCAILSPPP-----KPAAKQPANTFPDPPPLSISSNRWS---RMRSSRRE-KPGESL 60
           MGNLC++ +PP      KP  K+ ++         ++SNRW+   R  SSRR+ K  ++L
Sbjct: 1   MGNLCSLFTPPKPVKKRKPITKRQSSIGASSSGSGLNSNRWNNRVRSSSSRRDNKFEDAL 60

Query: 61  TPEQALEVAAAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLL 120
             E AL  AA    Q+     SLPFDRS S R      K +N LPRSSSSR RS TDPLL
Sbjct: 61  IQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSK-KNQLPRSSSSRSRSSTDPLL 120

Query: 121 QPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSF 180
           QPHQ +NQ IKLDDLETNHFVLVHGG FGAWCWYKTIALLEE G++ TAIDL G GI+S 
Sbjct: 121 QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSI 180

Query: 181 DPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAA 240
           + N + +L+QYV+PLTD LEKLP  EKVILVGHDFGGACISY MELFP KI+KA+F+AAA
Sbjct: 181 NINGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAA 240

Query: 241 MLSDGQNTLDMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKD 300
           ML++GQ+TLDMFSL+AG +D+MR+AQ+F+Y NGN   PTAI+L K LLKDL FNQ+P+KD
Sbjct: 241 MLTNGQSTLDMFSLKAGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKD 300

Query: 301 VALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQV 360
           VALASVSMR IPF PVLEKL LSDA Y SVRR+YI+TL DNA+P  +Q ++I  +PPE+V
Sbjct: 301 VALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKV 360

Query: 361 FSLKGADHSPFFSKPQALHRLL 374
           + LKGADH+PFFSKPQALH+LL
Sbjct: 361 YRLKGADHAPFFSKPQALHKLL 381

BLAST of Moc08g36860 vs. ExPASy Swiss-Prot
Match: F4I0K9 (Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES15 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.7e-102
Identity = 190/346 (54.91%), Postives = 253/346 (73.12%), Query Frame = 0

Query: 49  ESLTPEQALEVAAAALYQKRPLDSSL--PFDRSTSLRQPAP-----------------GK 108
           +SL  +Q L  A   L+ + P +S+   PF RSTS+    P                  K
Sbjct: 88  DSLIQDQTL-AATNILFSQTPRNSNSAPPFRRSTSVVYTQPPTAAVAASVGSVSGALTPK 147

Query: 109 KNRNALPRSSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIA 168
           K+     RSSS+R RS TDP+L+P+QL+++++K++  ET  FVLVHGGGFGAWCWYKTI 
Sbjct: 148 KSTYGYVRSSSNRQRSSTDPVLKPNQLLDKELKVEGAETKRFVLVHGGGFGAWCWYKTIT 207

Query: 169 LLEEAGYRATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGA 228
           LLE+ G++  A+DLTGSG+ SFD N++ +LAQYV+PL  F + L   EKVILVGHDFGGA
Sbjct: 208 LLEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFFDTLKPTEKVILVGHDFGGA 267

Query: 229 CISYEMELFPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGSD-DVMRQAQVFVYANGNGKT 288
           C+SY ME++P KIAKAIFI+AAML++ Q+TLD+F+ Q  S+ D+M Q  +F+YANG    
Sbjct: 268 CMSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQVHLFLYANGKKNP 327

Query: 289 PTAIELKKPLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQT 348
           PTA++  + LL+D FFNQ+P KDVALASVSMRPIPF PV+EKLH+S+  Y S+RRFYI+T
Sbjct: 328 PTAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEKNYGSIRRFYIKT 387

Query: 349 LNDN-AVPAPIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
           + D+ AVP  +Q ++I  NPPEQVF LKG+DH+PFFS+PQ+L+R+L
Sbjct: 388 MEDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRIL 432

BLAST of Moc08g36860 vs. ExPASy Swiss-Prot
Match: F4IE65 (Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES13 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 4.0e-101
Identity = 185/340 (54.41%), Postives = 249/340 (73.24%), Query Frame = 0

Query: 54  EQALEVAAAALYQKRPLDSSL----PFDRSTSLRQPA---------------PGKKNRNA 113
           EQ L  A   L+ + P +S+      F RSTS+  P+                 KK+   
Sbjct: 94  EQTL-AATNLLFNQTPRNSNSVVPPSFRRSTSVVYPSAQPSGTSSGPVSAVQTPKKSSAG 153

Query: 114 LPRSSSSRPRSLTDPLLQPHQLVNQDI-KLDDLETNHFVLVHGGGFGAWCWYKTIALLEE 173
             RSSSSR RS TDP+++P+QLV++++ K++  ET  FVLVHGGGFGAWCWYKTI LLE+
Sbjct: 154 FVRSSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYKTITLLEK 213

Query: 174 AGYRATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISY 233
            G++  A++LTGSG+ S D N++ +LA Y +PL  F E L   EKVILVGHDFGGAC+SY
Sbjct: 214 HGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEKVILVGHDFGGACMSY 273

Query: 234 EMELFPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIE 293
            ME+FP KIAKA+FI+AAML++GQ+TLD+F+ Q GS+D+M+QAQ+F+YANG    PTA++
Sbjct: 274 AMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDLMQQAQIFLYANGKKNPPTAVD 333

Query: 294 LKKPLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNA 353
             + LL+D  FNQ+P KD+ALASVS+RPIPF PV EK+H+S+  Y S+RRFYI+T+ D A
Sbjct: 334 FDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKTMEDYA 393

Query: 354 VPAPIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
           VP  +Q ++I  NPPEQVF LKG+DH+PFFS+PQ+L+++L
Sbjct: 394 VPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKIL 432

BLAST of Moc08g36860 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-78
Identity = 141/277 (50.90%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 98  SSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYR 157
           S+S+R R+L+DP     Q+ +     + L    FVLVHG GFGAWCWYK +A LEE+G  
Sbjct: 68  STSTRKRTLSDPFSNGKQVPDFS---ESLIVKKFVLVHGEGFGAWCWYKMVASLEESGLS 127

Query: 158 ATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMEL 217
              +DLTG G +  D N+V  L +Y +PL D LE LP++EKVILVGH  GGA ISY +E 
Sbjct: 128 PVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALER 187

Query: 218 FPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGS-DDVMRQAQVFVYANGNGKTPTAIELKK 277
           FP KI+KAIF+ A M+SDGQ   D+FS + GS +  M+++Q  +Y NG  K PT    +K
Sbjct: 188 FPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEK 247

Query: 278 PLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPA 337
           P +K L+FNQ+P KD+ALA +SMRP+P  P++EK+ L+  +Y   RRFY+QTL+D A+  
Sbjct: 248 PHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSP 307

Query: 338 PIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
            +Q  L+  N PE VF +KG+DH PFFSKPQ+LH++L
Sbjct: 308 DVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKIL 341

BLAST of Moc08g36860 vs. ExPASy Swiss-Prot
Match: Q940H7 (Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES12 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.7e-75
Identity = 138/277 (49.82%), Postives = 187/277 (67.51%), Query Frame = 0

Query: 98  SSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYR 157
           S+SSR  +L+D      Q+      L+ L+   FVLVHG GFGAWCWYKTIA LEE+G  
Sbjct: 69  STSSRRGTLSDSFSNNKQVPE---FLESLKVKKFVLVHGEGFGAWCWYKTIASLEESGLS 128

Query: 158 ATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMEL 217
              +DL GSG +  D NSV  L +Y +PL + ++ LP +EKVILVGH  GGAC+SY +E 
Sbjct: 129 PVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVSYALER 188

Query: 218 FPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGS-DDVMRQAQVFVYANGNGKTPTAIELKK 277
           FP KI+KAIFI A M++DGQ   D+F+ + GS +  M+++Q  +Y NG     T    +K
Sbjct: 189 FPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATGFMFEK 248

Query: 278 PLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPA 337
             +K L+FNQ+P KD+AL+ +SMRP+P  P++EKL LS  +Y   RRFY+QTL+D A+  
Sbjct: 249 QHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDDLALSP 308

Query: 338 PIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
            +Q  L+  N PE VF +KG+DH PFFSKPQ+LH++L
Sbjct: 309 DVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKIL 342

BLAST of Moc08g36860 vs. ExPASy TrEMBL
Match: A0A6J1DXF2 (putative methylesterase 11, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111024985 PE=4 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 2.6e-212
Identity = 375/379 (98.94%), Postives = 377/379 (99.47%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA
Sbjct: 72  MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 131

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD
Sbjct: 132 AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 191

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA
Sbjct: 192 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 251

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL
Sbjct: 252 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 311

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR
Sbjct: 312 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 371

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS
Sbjct: 372 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 431

Query: 361 PFFSKPQALHRLLFVDLDR 380
           PFFSKPQALHRLL V++ R
Sbjct: 432 PFFSKPQALHRLL-VEISR 449

BLAST of Moc08g36860 vs. ExPASy TrEMBL
Match: A0A6J1KBM9 (putative methylesterase 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111492839 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.1e-176
Identity = 317/373 (84.99%), Postives = 333/373 (89.28%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  S PPKP  KQP NTFP+PPPLSISSNRW R RSSRREK GES++ EQAL VA
Sbjct: 1   MGNLCATFS-PPKPVVKQPENTFPNPPPLSISSNRWFRTRSSRREKVGESMSSEQAL-VA 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P+D S  FDRSTSLRQP  G KNRN LPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKHPMDCS--FDRSTSLRQPPSGSKNRNVLPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEE GYRATAIDLTGSG+HSFDPNS+ +L+
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEVGYRATAIDLTGSGVHSFDPNSITDLS 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAMLSDGQNTL
Sbjct: 181 QYVQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHFKIAKAVFIAAAMLSDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMF+LQAG+DDVMRQAQVFVYANGN   PT IELKKPLLKDLFFNQT  KDVALASVSMR
Sbjct: 241 DMFTLQAGADDVMRQAQVFVYANGNDNAPTTIELKKPLLKDLFFNQTTAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKL LS+ KY SVRRFYIQTLNDNA+P  IQ +LI R+PPEQVF LKGADHS
Sbjct: 301 PIPFPPVLEKLRLSETKYGSVRRFYIQTLNDNAIPVSIQETLIERSPPEQVFILKGADHS 360

Query: 361 PFFSKPQALHRLL 374
           PFFSKPQALHRLL
Sbjct: 361 PFFSKPQALHRLL 369

BLAST of Moc08g36860 vs. ExPASy TrEMBL
Match: A0A6J1GAX7 (putative methylesterase 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452361 PE=4 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 8.7e-176
Identity = 316/373 (84.72%), Postives = 333/373 (89.28%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  S PPKP  KQP NTFP+PPPLSISSNRW R RSSRREK GES++ EQAL  A
Sbjct: 1   MGNLCATFS-PPKPVVKQPENTFPNPPPLSISSNRWFRTRSSRREKVGESMSSEQAL-AA 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P+D S  FDRSTSLRQP  G+KNRN LPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKHPMDCS--FDRSTSLRQPPSGRKNRNVLPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEE GYRATAIDLTGSG+HSFDPNS+ +L+
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEVGYRATAIDLTGSGVHSFDPNSITDLS 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QYVQPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAMLSDGQNTL
Sbjct: 181 QYVQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHFKIAKAVFIAAAMLSDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAG+DDVMRQAQVFVYANGN   PT IELKKPLLKDLFFNQT  KDVALASVSMR
Sbjct: 241 DMFSLQAGADDVMRQAQVFVYANGNDNAPTTIELKKPLLKDLFFNQTTAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           PIPFPPVLEKL LS+ +Y SVRRFYIQTLNDNA+P  IQ +LI R+PPEQVF LKGADHS
Sbjct: 301 PIPFPPVLEKLRLSETRYGSVRRFYIQTLNDNAIPVSIQETLIERSPPEQVFILKGADHS 360

Query: 361 PFFSKPQALHRLL 374
           PFFSKPQALHRLL
Sbjct: 361 PFFSKPQALHRLL 369

BLAST of Moc08g36860 vs. ExPASy TrEMBL
Match: A0A0A0L0Y0 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G129620 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 7.6e-172
Identity = 311/379 (82.06%), Postives = 331/379 (87.34%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  SPP     K P  T P+P   SISSNRWSRMRSSRR   G+SLT + AL+ A
Sbjct: 1   MGNLCATFSPP-----KPPPTTLPNPSSFSISSNRWSRMRSSRRHDAGDSLTSDHALD-A 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK P D S+ FDRSTSLRQP  GKKNRNALPRSSSSR RSLTDPLLQPHQLVNQD
Sbjct: 61  AAALFQKLPPDCSVSFDRSTSLRQPTSGKKNRNALPRSSSSRARSLTDPLLQPHQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNS+ +LA
Sbjct: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSITDLA 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QY QPL D LEKLPD +KVILVGHDFGGACISY MELF  KIAKA+F+AAAML+DGQNTL
Sbjct: 181 QYTQPLIDLLEKLPDGKKVILVGHDFGGACISYAMELFHSKIAKAVFVAAAMLNDGQNTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAGSDDVM+QAQVFVY+NGN   PTAIELKKPLLKDLFFNQTP KDVALASVSMR
Sbjct: 241 DMFSLQAGSDDVMQQAQVFVYSNGNDNPPTAIELKKPLLKDLFFNQTPAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           P+PFPPVLEKL LS+ KY SVRRFYIQTLNDNA+P PIQ SLI RNPPEQVF LKGADHS
Sbjct: 301 PVPFPPVLEKLRLSEKKYGSVRRFYIQTLNDNAIPVPIQESLIERNPPEQVFYLKGADHS 360

Query: 361 PFFSKPQALHRLLFVDLDR 380
           PFFSKPQALHR LFV++ +
Sbjct: 361 PFFSKPQALHR-LFVEISK 372

BLAST of Moc08g36860 vs. ExPASy TrEMBL
Match: A0A5D3BWN4 (Putative methylesterase 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold121G001150 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.9e-171
Identity = 312/379 (82.32%), Postives = 331/379 (87.34%), Query Frame = 0

Query: 1   MGNLCAILSPPPKPAAKQPANTFPDPPPLSISSNRWSRMRSSRREKPGESLTPEQALEVA 60
           MGNLCA  SPP     K P  T P+P   SIS+NRWSRMRSSRR   G+SLT +QAL+ A
Sbjct: 1   MGNLCATFSPP-----KPPPTTLPNPASFSISTNRWSRMRSSRRHNAGDSLTSDQALD-A 60

Query: 61  AAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLLQPHQLVNQD 120
           AAAL+QK PLD SL FDRSTSLRQP  GKKNRNALPRSSSSR RSLTDPLLQP QLVNQD
Sbjct: 61  AAALFQKPPLDCSLSFDRSTSLRQPTSGKKNRNALPRSSSSRARSLTDPLLQPQQLVNQD 120

Query: 121 IKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSVVNLA 180
           IKLDDLET HFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNS+ +LA
Sbjct: 121 IKLDDLETIHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSFDPNSITDLA 180

Query: 181 QYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAAMLSDGQNTL 240
           QY QPLTDFLEKLPD EKVILVGHDFGGACISY MELF  KIAKA+FIAAAML+DG+NTL
Sbjct: 181 QYTQPLTDFLEKLPDGEKVILVGHDFGGACISYAMELFHSKIAKAVFIAAAMLNDGENTL 240

Query: 241 DMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKDVALASVSMR 300
           DMFSLQAGS +VM+QAQVFVY+NGN   PTAIELKKPLLKDLFFNQTP KDVALASVSMR
Sbjct: 241 DMFSLQAGSGEVMQQAQVFVYSNGNENPPTAIELKKPLLKDLFFNQTPAKDVALASVSMR 300

Query: 301 PIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQVFSLKGADHS 360
           P+PF PVLEKL LSD KY SVRRFYIQTLNDNA+P PIQ SLI RNPPEQVF LKGADHS
Sbjct: 301 PVPFSPVLEKLRLSDTKYGSVRRFYIQTLNDNAIPVPIQESLIERNPPEQVFYLKGADHS 360

Query: 361 PFFSKPQALHRLLFVDLDR 380
           PFFSKPQALHR LFV++ +
Sbjct: 361 PFFSKPQALHR-LFVEISK 372

BLAST of Moc08g36860 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 471.5 bits (1212), Expect = 6.9e-133
Identity = 244/382 (63.87%), Postives = 293/382 (76.70%), Query Frame = 0

Query: 1   MGNLCAILSPPP-----KPAAKQPANTFPDPPPLSISSNRWS---RMRSSRRE-KPGESL 60
           MGNLC++ +PP      KP  K+ ++         ++SNRW+   R  SSRR+ K  ++L
Sbjct: 1   MGNLCSLFTPPKPVKKRKPITKRQSSIGASSSGSGLNSNRWNNRVRSSSSRRDNKFEDAL 60

Query: 61  TPEQALEVAAAALYQKRPLDSSLPFDRSTSLRQPAPGKKNRNALPRSSSSRPRSLTDPLL 120
             E AL  AA    Q+     SLPFDRS S R      K +N LPRSSSSR RS TDPLL
Sbjct: 61  IQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSK-KNQLPRSSSSRSRSSTDPLL 120

Query: 121 QPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYRATAIDLTGSGIHSF 180
           QPHQ +NQ IKLDDLETNHFVLVHGG FGAWCWYKTIALLEE G++ TAIDL G GI+S 
Sbjct: 121 QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSI 180

Query: 181 DPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMELFPLKIAKAIFIAAA 240
           + N + +L+QYV+PLTD LEKLP  EKVILVGHDFGGACISY MELFP KI+KA+F+AAA
Sbjct: 181 NINGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAA 240

Query: 241 MLSDGQNTLDMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIELKKPLLKDLFFNQTPTKD 300
           ML++GQ+TLDMFSL+AG +D+MR+AQ+F+Y NGN   PTAI+L K LLKDL FNQ+P+KD
Sbjct: 241 MLTNGQSTLDMFSLKAGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKD 300

Query: 301 VALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPAPIQASLITRNPPEQV 360
           VALASVSMR IPF PVLEKL LSDA Y SVRR+YI+TL DNA+P  +Q ++I  +PPE+V
Sbjct: 301 VALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKV 360

Query: 361 FSLKGADHSPFFSKPQALHRLL 374
           + LKGADH+PFFSKPQALH+LL
Sbjct: 361 YRLKGADHAPFFSKPQALHKLL 381

BLAST of Moc08g36860 vs. TAIR 10
Match: AT1G69240.1 (methyl esterase 15 )

HSP 1 Score: 372.9 bits (956), Expect = 3.3e-103
Identity = 190/346 (54.91%), Postives = 253/346 (73.12%), Query Frame = 0

Query: 49  ESLTPEQALEVAAAALYQKRPLDSSL--PFDRSTSLRQPAP-----------------GK 108
           +SL  +Q L  A   L+ + P +S+   PF RSTS+    P                  K
Sbjct: 88  DSLIQDQTL-AATNILFSQTPRNSNSAPPFRRSTSVVYTQPPTAAVAASVGSVSGALTPK 147

Query: 109 KNRNALPRSSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIA 168
           K+     RSSS+R RS TDP+L+P+QL+++++K++  ET  FVLVHGGGFGAWCWYKTI 
Sbjct: 148 KSTYGYVRSSSNRQRSSTDPVLKPNQLLDKELKVEGAETKRFVLVHGGGFGAWCWYKTIT 207

Query: 169 LLEEAGYRATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGA 228
           LLE+ G++  A+DLTGSG+ SFD N++ +LAQYV+PL  F + L   EKVILVGHDFGGA
Sbjct: 208 LLEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFFDTLKPTEKVILVGHDFGGA 267

Query: 229 CISYEMELFPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGSD-DVMRQAQVFVYANGNGKT 288
           C+SY ME++P KIAKAIFI+AAML++ Q+TLD+F+ Q  S+ D+M Q  +F+YANG    
Sbjct: 268 CMSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQVHLFLYANGKKNP 327

Query: 289 PTAIELKKPLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQT 348
           PTA++  + LL+D FFNQ+P KDVALASVSMRPIPF PV+EKLH+S+  Y S+RRFYI+T
Sbjct: 328 PTAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEKNYGSIRRFYIKT 387

Query: 349 LNDN-AVPAPIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
           + D+ AVP  +Q ++I  NPPEQVF LKG+DH+PFFS+PQ+L+R+L
Sbjct: 388 MEDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRIL 432

BLAST of Moc08g36860 vs. TAIR 10
Match: AT1G26360.1 (methyl esterase 13 )

HSP 1 Score: 369.8 bits (948), Expect = 2.8e-102
Identity = 185/340 (54.41%), Postives = 249/340 (73.24%), Query Frame = 0

Query: 54  EQALEVAAAALYQKRPLDSSL----PFDRSTSLRQPA---------------PGKKNRNA 113
           EQ L  A   L+ + P +S+      F RSTS+  P+                 KK+   
Sbjct: 94  EQTL-AATNLLFNQTPRNSNSVVPPSFRRSTSVVYPSAQPSGTSSGPVSAVQTPKKSSAG 153

Query: 114 LPRSSSSRPRSLTDPLLQPHQLVNQDI-KLDDLETNHFVLVHGGGFGAWCWYKTIALLEE 173
             RSSSSR RS TDP+++P+QLV++++ K++  ET  FVLVHGGGFGAWCWYKTI LLE+
Sbjct: 154 FVRSSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYKTITLLEK 213

Query: 174 AGYRATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISY 233
            G++  A++LTGSG+ S D N++ +LA Y +PL  F E L   EKVILVGHDFGGAC+SY
Sbjct: 214 HGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEKVILVGHDFGGACMSY 273

Query: 234 EMELFPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGSDDVMRQAQVFVYANGNGKTPTAIE 293
            ME+FP KIAKA+FI+AAML++GQ+TLD+F+ Q GS+D+M+QAQ+F+YANG    PTA++
Sbjct: 274 AMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDLMQQAQIFLYANGKKNPPTAVD 333

Query: 294 LKKPLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNA 353
             + LL+D  FNQ+P KD+ALASVS+RPIPF PV EK+H+S+  Y S+RRFYI+T+ D A
Sbjct: 334 FDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKTMEDYA 393

Query: 354 VPAPIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
           VP  +Q ++I  NPPEQVF LKG+DH+PFFS+PQ+L+++L
Sbjct: 394 VPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKIL 432

BLAST of Moc08g36860 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-79
Identity = 141/277 (50.90%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 98  SSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYR 157
           S+S+R R+L+DP     Q+ +     + L    FVLVHG GFGAWCWYK +A LEE+G  
Sbjct: 68  STSTRKRTLSDPFSNGKQVPDFS---ESLIVKKFVLVHGEGFGAWCWYKMVASLEESGLS 127

Query: 158 ATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMEL 217
              +DLTG G +  D N+V  L +Y +PL D LE LP++EKVILVGH  GGA ISY +E 
Sbjct: 128 PVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEKVILVGHSTGGASISYALER 187

Query: 218 FPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGS-DDVMRQAQVFVYANGNGKTPTAIELKK 277
           FP KI+KAIF+ A M+SDGQ   D+FS + GS +  M+++Q  +Y NG  K PT    +K
Sbjct: 188 FPEKISKAIFVCATMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEK 247

Query: 278 PLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPA 337
           P +K L+FNQ+P KD+ALA +SMRP+P  P++EK+ L+  +Y   RRFY+QTL+D A+  
Sbjct: 248 PHMKGLYFNQSPNKDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSP 307

Query: 338 PIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
            +Q  L+  N PE VF +KG+DH PFFSKPQ+LH++L
Sbjct: 308 DVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSLHKIL 341

BLAST of Moc08g36860 vs. TAIR 10
Match: AT4G09900.1 (methyl esterase 12 )

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-76
Identity = 138/277 (49.82%), Postives = 187/277 (67.51%), Query Frame = 0

Query: 98  SSSSRPRSLTDPLLQPHQLVNQDIKLDDLETNHFVLVHGGGFGAWCWYKTIALLEEAGYR 157
           S+SSR  +L+D      Q+      L+ L+   FVLVHG GFGAWCWYKTIA LEE+G  
Sbjct: 69  STSSRRGTLSDSFSNNKQVPE---FLESLKVKKFVLVHGEGFGAWCWYKTIASLEESGLS 128

Query: 158 ATAIDLTGSGIHSFDPNSVVNLAQYVQPLTDFLEKLPDDEKVILVGHDFGGACISYEMEL 217
              +DL GSG +  D NSV  L +Y +PL + ++ LP +EKVILVGH  GGAC+SY +E 
Sbjct: 129 PVTVDLAGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEKVILVGHSTGGACVSYALER 188

Query: 218 FPLKIAKAIFIAAAMLSDGQNTLDMFSLQAGS-DDVMRQAQVFVYANGNGKTPTAIELKK 277
           FP KI+KAIFI A M++DGQ   D+F+ + GS +  M+++Q  +Y NG     T    +K
Sbjct: 189 FPEKISKAIFICATMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATGFMFEK 248

Query: 278 PLLKDLFFNQTPTKDVALASVSMRPIPFPPVLEKLHLSDAKYRSVRRFYIQTLNDNAVPA 337
             +K L+FNQ+P KD+AL+ +SMRP+P  P++EKL LS  +Y   RRFY+QTL+D A+  
Sbjct: 249 QHMKGLYFNQSPNKDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDDLALSP 308

Query: 338 PIQASLITRNPPEQVFSLKGADHSPFFSKPQALHRLL 374
            +Q  L+  N PE VF +KG+DH PFFSKPQ+LH++L
Sbjct: 309 DVQEKLVRENSPEAVFKIKGSDHCPFFSKPQSLHKIL 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158512.15.3e-21298.94putative methylesterase 11, chloroplastic [Momordica charantia][more]
KAG6607066.14.7e-17684.99putative methylesterase 11, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_023524731.16.2e-17684.99putative methylesterase 11, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022998085.11.1e-17584.99putative methylesterase 11, chloroplastic [Cucurbita maxima][more]
XP_022948800.11.8e-17584.72putative methylesterase 11, chloroplastic [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FW039.7e-13263.87Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4I0K94.7e-10254.91Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4IE654.0e-10154.41Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q9FVW31.6e-7850.90Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q940H71.7e-7549.82Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
A0A6J1DXF22.6e-21298.94putative methylesterase 11, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1KBM95.1e-17684.99putative methylesterase 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1GAX78.7e-17684.72putative methylesterase 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0L0Y07.6e-17282.06AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G129... [more]
A0A5D3BWN42.9e-17182.32Putative methylesterase 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
Match NameE-valueIdentityDescription
AT3G29770.16.9e-13363.87methyl esterase 11 [more]
AT1G69240.13.3e-10354.91methyl esterase 15 [more]
AT1G26360.12.8e-10254.41methyl esterase 13 [more]
AT1G33990.11.2e-7950.90methyl esterase 14 [more]
AT4G09900.11.2e-7649.82methyl esterase 12 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 131..370
e-value: 2.1E-19
score: 71.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 123..375
e-value: 2.8E-63
score: 216.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 130..373
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..54
NoneNo IPR availablePANTHERPTHR10992:SF1025METHYLESTERASE 15, CHLOROPLASTIC-RELATEDcoord: 47..373
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 47..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g36860.1Moc08g36860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001732 formation of cytoplasmic translation initiation complex
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
cellular_component GO:0016282 eukaryotic 43S preinitiation complex
cellular_component GO:0033290 eukaryotic 48S preinitiation complex
cellular_component GO:0071541 eukaryotic translation initiation factor 3 complex, eIF3m
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity
molecular_function GO:0003743 translation initiation factor activity