Moc08g35890 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g35890
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description2-isopropylmalate synthase
Locationchr8: 26482383 .. 26490778 (+)
RNA-Seq ExpressionMoc08g35890
SyntenyMoc08g35890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGCGACGAGACGAAACCCATGAACAGCAATCGGAAAACAGCAGTGCTGGCGAATCAGCAAACGTCGACGGTCGGCGGCGGCGGCGGCGAGTTGTGTTGGATAGCAATGAACAAAACCCTAAGGCACAAAATCCTAATGGGCACAAAATCCTAATGGGCACAAAACCCTAATGGGCACAAAACCCTAATGGGCAGGGCACAAAAAGTTTAGAAGAAACCTAGAGCTCTGATACCATATAGAGACTAAGGTTGGAGAGAATCCAATGTAATGTATTCTCATTATTAAAGTCTATATCTATAGACAAATATACAAAGTTGACCTAATCCTATAAGGAGAAGGTAAAGGACAAATTAATATTAATACAATAATATAAAATATACTAATATCTATACTCTAATACAAGTCAAGCTTTATAACCACTCCATTTTTCTTTTTTCTAAAGCATTTGTCAATTATTTCATTGCCTATAAGGGATGTGTCTAGAATTTGCCTTCAGCAACAAAAGCTTATTGATATTCTATGATGATACTCGACAACACTTTTTTCAACCTTTGGATAGTACGCTTTTTTAACAATTGCAGCTTTCCAATCATTGTAGGCTATAACAAGTATTCCTTGTGGGTTTCTAAGGTTTCTATAGCTTTTTTCTGACCTTTGAGCTTCTCAATAAAACCACGTCCAAGCCATCCTCTAAGGGGGTTGTTTTTCCACCAGAAATTGTGGATACCAATTGTCAACATTATAGGATAATGGTCCGAGGTTGTACTCTCAAGCTTTTCTTAACCGATGCTTAAGATAATTTGGTGATGATGGACTCTAATAGAGGAAATGGTCAATGAGGATTAGTGTAGGCGAGTATCTGAAATCGGACCAAGTTACCCTTCCATCGGAAAGAGCTACATCTATGAGCCCGCCTGATTGATGAAGGTGTTGAACTTTCTCATAAACTTGGAGTGTCTTTTGCTTGAAGTATCTATTTTTGAATATTATAGGTAATAACTTATCAAAGTTGTTTTAACTTTGTTTTACTAGAGTTTTTCTTTTATTTTATTTCATGGACATATCTGAGGATAAATAGTCTATCCGTCATTCTACAATACTATTATTTCTTGTATGTAAAAGAGGATGAATATTAAATTTAGTGACAATAGTGTCAATTTTGTACAACTGTTCTTATCTAATTTTTTTTATACAACTGTTTTCCTTTATGAGTTAGATGTGACTGAATGCACGGTGCTGTTGAAGTGTTTTGAGAACATCTGTTTGCGCTGACATGTGTAGGTAGACTTCTTAAATGACATACATCTATTTTCTTCTTTTAAACCATATTTTTATATCTTCAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCGAGACATATCTTTCTGACTAGCAAGATGGTAATTATTTTTCTTACAAAGAAAAGCTTTTTGAAACTCGAGTTAATTTTTAAAAGAAAAATTATTCTTGTCATCCTTTTTTTTTAAATATTTTTTTAATAAAAAAAACGTACAAAGTTAAACGTTCCATAGGGCAGGATATTTCCTGTTGCAAGTTGTTTGTGGCCCTAGGTTGAACTTGAAAGCTAGACAGTTTTGTTCTTGCTTCTAGCTCCTCTTTGAAAGACTTGGAAAGAGAAGAAGGGGCAAAGTTTTTGAGGATGGGAACTGGGAAGAGTGTTTCTTTCTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGAGAGGTTTAATGATCCAACGTCCAAAGTCCTATTTATTTTTTATTTTTTTCATGACTATTGTTTCTTTTTAAAATCTGTTAGTTAGAACCGTTTTTGGTTTTTTTTGTTGTGGTTTAGTGTACTTCAGATTTTGAATTAACGACAGATTGCTGGTTCTAGTTCTCTCTTCGCCTGCACTTTCAATTTTTCTATTCAAGTTTCAACATCCGTATTTCTTATTAATAAAAGGCTACTTACGGACAAACTTATTCCTTAGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGTTAGACTTGTTAGATCTTGATATGCTAGGTTTTGTTGTTCATGGCTAACTATTATGACGTGTTTTCATATTTATCTTTCAAAATCCAGCGTGATGTGAAAGCTTCCAAATATACTCTCATAGCTGACTATGTCAAGGTTCTAGCATCAGTGTTTATTGCTTTAAACACATTTAAAGAAAACAGTTCGCCATTTTTTCCTTTAATTCAACTCTACCCTGTGAGTTAATTGAAAAAAATTTCTTTTTTTAGATTGTCAAATTCAAGAAACAGAGGTACAGAAGGACAGTAAGGGAAGATGATTCTTTCCCTATGAGGTGATGGAGATTACATTGGATTCTCCTCAATTCGGGAGAACGATGAGAGAGTGTGAAACACCTTCTAAAGCACTCCAAGAGAAAGGAAGAAAACAGTGTCTTCCTATATAGTGTTAAAATTTCTATCCAATCTGTAAGAAAATTTTCCAATTCCTTTCAAGCTGAGCGCTCTATATACTTGTAGTCTTTTGCTTGAAAGGGTGGCATAGCACTTAAGACCGATCCTCTTCATCCAAAGCCTAGTTGAATACAAGATGCAGCTGGTTGTATCTTGTAGTTTCATCCTGGCCTCCATTATTCTTGTACTTGAGTCATCAGGTGACAGGTATTTTTGGGGCCATAGAATGTATTTAATTGTTCATAAAGAACCAGTTGTTCATTACTAATTACTGTCCGAGGAGTTACATGATTGTTATAGAGCAGCTACGTTCTCTTATAAAAATGGAGGACTCTTTTAACTATTTTGATGCATAGTCTTGCCGTAAAGTTCATAATGAAAATATTGATTGGCTCTTGTTCTATGTTTTTAATAATTATTACTATTTTTGTTTGAGCCATGTGGGTGACTGTTTATCCTGTTTTGTTACGCTCAAATTGTATCTTAAATATTGCTTTTGTTACACAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGGTATTCCATGTTGCTTTATGGAAAATGACAATAGTGCTTTTATCTTGACAAAATCTCCCTACCTACCTCCCCTGCCTCCCCTTTCCCATCTCTCTATGTGTGTGTAAGCATGTCTGTGCCTGGTTAGAAGAAGAAGTATTTAGAGCATGTCTGTGCCTGGTTAGAAGAAGTAGTATTTAGTGCAGTTGTTCGCTGGGATTTATTTTGAGGCTATTTCGGGGGTTGTTTATTAGGGGCTCCGCATGAGGGCCAAAAAAAGGTGGGGGGAGTGAACAGCAGCAGACCATATGAAAATTAGAAAAAAGTATGTGAAAAATATAATATCGGGGCTTTACCTAAGAGATTGTTTAAAAATACTATGCAGCAACTTTTTTCCTTTTATAATAAAGAAGGGAACAATTGAAAATACCCAAAAAATCGTGCTGTGTACCTTACTGAGTTCACCTTGCCTCAGAGGAGGCAAGCCCCCTAAGGGCGGGTGCCTTGCAGCTGCTAATTATGCCTTTTAAGGTTTGGTATTGTCACTTTATATATGTACGGACATACACGTTCCTTTCTTCTCTCCTAGCTTTTTAAGTGTATATATTTTGGTTTGGTACCTTAAATAGTTTAGCATCTATGATTTATTTAATAATTAATATTTTAGTGTGTATATAAAAATTATTTTCACCCTTCTCTTAAGAAAGGTTTCATTAAAATATTGAGTGTGTTTCAGTGACATTCCAAGTGTCTGAGGTGTAAATTTCGGACAGTTTTCAAAATAGAATTTTTGTGCTTTCTGGAGGGCAAGTACCTCAACTAAAAGGATTAGAATCTAGTAGGCTGGGGACCATGATCAAAGGAAAACGTAGCAAGAGGCCAGCACTGCGAAAACTTTCTCTACCCATTCTATTTCTTGTTTTTCTCTTTCACTCTTCGGGAGTTTGTATCTTTGAACAATTTTTGTTTCTTTTCATATATCAATGAAAAGTTCGTATCTTGTTAAAAAAAAAAGTCCATTAACACAGAAATCCTGCAACAGAGTAGTCGTTCCTTGAGATTCTTTCAAGCTTGCAATAGGTTACCTTCTCTTCTTTCTTCTCAGTATACTATTCTTGTAGCATCTAATGGAACTTCAATTGAGTTATCTTTTGCTCTTCTTTGCTGTCCTGAATATAACTTTTTTTTTTTTTTTTTATCTCATTGAAACCCTTAAATATCATCTCTCATCTCCTTTCATACAACAAATTAATCATCTATTGAAGTAACTTATTATATTTAGTTTCTGGCAGAATTCCTGGGCATTCAAACTATCTACTATGAGTTTCTATTTATATGGCTGAGGTTCTAAGGATAATATGGTATTTTTCATCCTTTGCGTTGAAAACATCACACAACTCTTGCTTTTACAGTGTAGAGATTAAGGTTGGAGAGAATCCAATGTAATGTATTCTCATTATTAAAGTCTATATATATAGACAAATATACAAGGTTGACCTAATCCTATAAGGAGAAGGTAAAAGACAAATTAATATTAATACAAGAATATAAAATATACTAATATATACTCTAACACTCCCCCTCAAGTTGGTGCATATATATTAATCATGCGCAACTTGTTAGACAAATAATCTATTCGTGCTCCATTCAACTTGTGTTCTGTGATTTTTGATATCATTGGGATCACCTCAGAAGTTACGATTGATTTCTTCTCAGCCATAACAAACAAACGAACTTATTTGAACAAAACGACAAAACCAGACCAAACCACCCGAACCAAACAAAACATATGAATCAAACGGTTGAACCAAACCGGATTAGGTGGTTTCTGAAACCAGAAACCCCAAGTGCTACCCCACGAACAACCAGAGGGGCAAAGTCGACGGGTGGCGACTAGCGGTGACGAAAGCACACGGCGACGGCGATTGGTGGCGAGACGAAACCTACGTTCAGCAATCGGAAAACAGCAGTGCTGGCAAATCAGCAAACTGCGGTCGGCGGCGGCAGCGGCGGTCGGCGGTAGTAGTGAGTTGAGTTGGATAGCAATGAAAAACAAAAACCCTAAGGGACAAAATCCTAATGGGCATCAAAACCCTAATGGGCACAAAACCTTAATGGGCAAGAAACTTAGAGCTCTGATACCATGTAGAGACTAAATTTGGAGAGAATCCAATGTAATGTATTCTTATTATTAAAGTCTATATATATAGACAAATATACAAGGTTGACTTAATCCTATAAGGAGAAGGCAAAGGACAAATTAATATTAATACAAGAATATAAAATATACTAATATATAGTATATACTCTAACATACAGATATACTGTTCAAGAGAATCCTAGTATGCCATAGAGGTAGAACCTTTCTGCAGCACTTGAAACACCTCTTTGTTGAGATTCCTTTAGTACACGAAGAGGATCATAGTAACATTGGTAGTATGTTTAATTGCTTAAGAACATAGAAGAGGAGATTGAAGAAGGTATCTTGCAAGAACATGCATAATTTGTAAGGCATTTTCATAAATTCAAGGTCAGATCAATTTTAAGAATATTTGAATACAAATGGAAAAACCGGCCCCTTTTAGCTGTAAAATATCGTATAGTTTGGTACAATGATGCAGCTGTACCTAGGGAGCTGAAGTATATCTGGGACAGGAGGGATGAGGAATATCTGTAGCCATTCTATTGTGGCGTTGAAACTTTAAATTATTTTGTTACAACATCAACAGGATTGACTTCCTTTTGTTTATAATTTCACCCCTTGTATCTGGTCCGAAGTATGCCACCTGCTAACATGGTTCATTTTGACAATTTTTACAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGGTAAATTGGGATCTTCAAGCTCAATGGTTGTGTAATCTTTCTGTATTATGTCTTCCTTTCTGATAATATGTTTACGTTATATCCCATTTTTTCAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGAAGTAGCTGAACAAAAGAAGGTAACTTGAAATTTTGTCCCTCTCTGTTTTCTGAGCGAATTCCCTCCTCTGCCTCCTGCACGTATCTGTAAATACAAAAAAGGATTCTACTTTGGGTGCGATCATACCGGTGCATGATCCCATCAGAACTCTGTAGTTAAGTCTGCTTGGGCGAGAGTAGTACTAAATTGAGTGGCTACTTAGAAAGCTTTCACGTTGCACCCCTTTCAAACTTATTAAAAAAAAAAAAGAAGAAGCTCGTACTTCAACCTATTTCAAGAACATAAATTACCATTCATCAGGAAAGTTTTTTCATATAATTTCTTGCCTTTTTCCCTAATTTTCAGATATTTAGATCTACATTATTTTGCTCTAATTTATATGACATTCCAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCTGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTTTAAATTCTGTTATAGATCATTATATTTGGGAAGTTGTTTGGACCTACTTTTAAATACACATATAGCATCATTTGACAATACCTCAAATGCAGGTCACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTGAAACTCCTCGATGCTGACGGGAAAGAGCATATTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGTTTGTATAGGAGAAACTGCTAGCAAACTTTTTATATTTTCCATTTACATCTGGAATACACTTACAGGAAGATTTCCTGGTAAAGTCAAACTAGGATATCTTAGATAATTTGTTTTATGTTATTCTTGGCTTCCGTCTAAGAAGTCCCTGAATTTTAGAAGTGTCTAATAAATAGGTTATAACATAAAAGTCCTCGTGATCGATAAACCAGGCTTTCACATCCTTTGCATGTGTATCTAGTTCTGGGGTATTACTGAATGATTAACTATGATGTTGTCTTCTTGCCATAAATTTCTTGCTGGTGGTCCAGTGTGTCTTCCTTGAGTGAATCAGACGGTACAAGAGTTATCATTTTTTTTTAATGCAGGAACCAGTTACACTTCTCGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCAACTAATGCAATGACAGGAGAAGCAGTTCAACGAACATTTAGGTAAACTAGCTACAGGATAATGTGATACCTGCTTATTTTCTTTTAAAGGGAAAATTGCAAAATCCACCCCAAGATTTTAGGAGTTGTTACAATTGCACTTTTGCACCATCAGTTGATCGGTTACCCACTAAACTTATGAAATTGTTCGAATTAAACCCTTAAATATTACAAAATGCACAAACCACCCTTAAACCCTTAAAACTACAAAGATTGTTACCAACCCCTAGTTGATGGGTGAGTTTTGCAATTTTTCCTTTTTTAAAATACAATCAATCAAGTATTCTTGTTTGAAGTATTATTCCCTCTGCGATATTACATCTAAACGTCATTAATATTCAACTCGAAAAGATTTGGGATGGAGAATTATCAAATGCTATATCAATCAATCCATATCAAGAACTTCAAAGATGAATCGGGGCTGAATTACGTACGTCTAGCAAGCTATAGCATATCGATAAATGTATGTCTAGTGATCTGTTTTTTATTGTTGCAGTGGTATCGGGGCAGGCATGGACATAGTTGAATCAAGTGTCAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGTGACTGAAAAAAGAACATTGTCTGCATAAAAAATATTGATTTGAGCAGGAAGAATGATGATGGTGGCAATCTTGCATAAAAGCTTCCAGAATTTTGCTCATGAAATTTTCGACCGAGTAAGTTTCGGGATCTTATATCGTTTGTAATTTAGTTGTTTGCGTGTTCTTTGAAACCTTTTCAGAAGTACCTGCTTTAATGTTGTAGTTTGTAAACAGTGAACTGGCTTTAATTATCATTTTATGAATGAACCCTTTTCAGAAGTACCTGCTTGAATGTAAGTCATCTATCAAATGATAACATTTTGATATTTTTTTTATATGCATTCAAAACTTAAAGTTTGTTTTAGTTATTTTTACTGTTGGTTTTGAAATGCTGTTCTCTATCAGGCATTGACCACTGAACCAGCCCAATTGGTGAAACCTAATGTCTTTGTTATTTTATCGTAG

mRNA sequence

ATGATTGGCGACGAGACGAAACCCATGAACAGCAATCGGAAAACAGCAGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCGAGACATATCTTTCTGACTAGCAAGATGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGAAGTAGCTGAACAAAAGAAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCTGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTCACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTGAAACTCCTCGATGCTGACGGGAAAGAGCATATTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGAACCAGTTACACTTCTCGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCAACTAATGCAATGACAGGAGAAGCAGTTCAACGAACATTTAGTGGTATCGGGGCAGGCATGGACATAGTTGAATCAAGTGTCAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGCATTGACCACTGAACCAGCCCAATTGGTGAAACCTAATGTCTTTGTTATTTTATCGTAG

Coding sequence (CDS)

ATGATTGGCGACGAGACGAAACCCATGAACAGCAATCGGAAAACAGCAGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATTCTGGAATTAACTCGAGACATATCTTTCTGACTAGCAAGATGGTAGAAGAATATACTGGCTTGAATGTACAACCGCACAAGGCTATTGTTGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTTAGTGGACGCCATGCCCTTAAAAGTCGACTTCAAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACATATTCTGGCGTTTCAAAGAAGTAGCTGAACAAAAGAAGAGAGTAACAGATGCTGATCTTCGAGCATTGGTATCTGATGAAGTTTTTCAACCAAGTGTTGTCTGGAAGCTGCTAGATTTGCAGGTCACCTGTGGAACTCTTGGTCTTTCTACGGCTACCGTGAAACTCCTCGATGCTGACGGGAAAGAGCATATTAATTGTGCTGTTGGAACGGGGCCTGTAGATTCAGCTTACAAGGCTGTAGATGGCATTGTGAAGGAACCAGTTACACTTCTCGAGTATTCGATGAACGCTGTCACTGAAGGCATTGATGCAATTGCCACGACCCGTGTTGTAATAAGAGGGGAAAACAGTTACACATCAACTAATGCAATGACAGGAGAAGCAGTTCAACGAACATTTAGTGGTATCGGGGCAGGCATGGACATAGTTGAATCAAGTGTCAAGGCCTACATTGGTGCACTAAACAAGATGCTTGGTTTTCAAGGAGTTGACATTAAGGCATTGACCACTGAACCAGCCCAATTGGTGAAACCTAATGTCTTTGTTATTTTATCGTAG

Protein sequence

MIGDETKPMNSNRKTAVVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIKALTTEPAQLVKPNVFVILS
Homology
BLAST of Moc08g35890 vs. NCBI nr
Match: XP_022141882.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 550.4 bits (1417), Expect = 9.9e-153
Identity = 278/284 (97.89%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ
Sbjct: 330 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 389

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW
Sbjct: 390 DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 449

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL+ADGKE
Sbjct: 450 RFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKE 509

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H NCAVGTGPVDSAYKAVDGIVKEPV LLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN
Sbjct: 510 HTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 569

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQGVDIK
Sbjct: 570 AITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 613

BLAST of Moc08g35890 vs. NCBI nr
Match: XP_022141881.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 550.4 bits (1417), Expect = 9.9e-153
Identity = 278/284 (97.89%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ
Sbjct: 330 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 389

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW
Sbjct: 390 DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 449

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL+ADGKE
Sbjct: 450 RFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKE 509

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H NCAVGTGPVDSAYKAVDGIVKEPV LLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN
Sbjct: 510 HTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 569

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQGVDIK
Sbjct: 570 AITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 613

BLAST of Moc08g35890 vs. NCBI nr
Match: XP_038875433.1 (2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 529.3 bits (1362), Expect = 2.4e-146
Identity = 265/284 (93.31%), Postives = 276/284 (97.18%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVM+LQCRGEHVLGGLH+GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ
Sbjct: 338 VVMSLQCRGEHVLGGLHTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 397

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEIISPEDIGYERSN+AGIVLGKLSGRHALKSRL ELGYELD+ENLDN+FW
Sbjct: 398 DGMLKHKGTYEIISPEDIGYERSNEAGIVLGKLSGRHALKSRLLELGYELDSENLDNVFW 457

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLLDADGKE
Sbjct: 458 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKE 517

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI CAVGTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ SYT+TN
Sbjct: 518 HIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTATN 577

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGF GVDIK
Sbjct: 578 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFHGVDIK 621

BLAST of Moc08g35890 vs. NCBI nr
Match: XP_008457079.1 (PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 265/295 (89.83%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQ
Sbjct: 343 VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 402

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+ENL N+FW
Sbjct: 403 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFW 462

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLLDADGKE
Sbjct: 463 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKE 522

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI C+VGTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ SYTSTN
Sbjct: 523 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 582

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIKALTTEPAQLVK 312
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQG+DIK  T E   L+K
Sbjct: 583 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKE-TKEKHYLLK 636

BLAST of Moc08g35890 vs. NCBI nr
Match: XP_011651428.1 (2-isopropylmalate synthase 2, chloroplastic [Cucumis sativus] >KGN57885.1 hypothetical protein Csa_010376 [Cucumis sativus])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 261/284 (91.90%), Postives = 274/284 (96.48%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLH+GINSRHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQ
Sbjct: 341 VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 400

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKS L ELGYELD ENLDN+FW
Sbjct: 401 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFW 460

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLD+QVTCGTLGLSTATVKLLDADGKE
Sbjct: 461 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKE 520

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI C+VGTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ SYTSTN
Sbjct: 521 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 580

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQG+DIK
Sbjct: 581 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 624

BLAST of Moc08g35890 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 7.7e-132
Identity = 230/279 (82.44%), Postives = 258/279 (92.47%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQ
Sbjct: 335 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQ 394

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+FW
Sbjct: 395 DGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFW 454

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADL ALVSDEVFQP  VWKLLD+Q+TCGTLGLST+TVKL D+DGKE
Sbjct: 455 RFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKE 514

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H+ C+VGTGPVD+AYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+N+Y+STN
Sbjct: 515 HVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTN 574

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQ 296
           A+TGE+V+RTFSG GAGMDIV SSVKAY+GALNKMLGF+
Sbjct: 575 AVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

BLAST of Moc08g35890 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 471.1 bits (1211), Expect = 1.0e-131
Identity = 231/279 (82.80%), Postives = 254/279 (91.04%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQ
Sbjct: 337 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQ 396

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEII PE+IG ERSNDAGIVLGKLSGRHALK RL ELGY+LD+E L  IFW
Sbjct: 397 DGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQLSTIFW 456

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDAD+ ALVSDEVFQP  VWKLLD+Q+TCGTLGLSTATVKL DADGKE
Sbjct: 457 RFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKE 516

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H+ C++GTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG N Y+STN
Sbjct: 517 HVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTN 576

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQ 296
           A+TGE VQRTFSG GAGMDIV SSVKAY+GALNKM+ F+
Sbjct: 577 AITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFK 615

BLAST of Moc08g35890 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.0e-117
Identity = 207/294 (70.41%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMAL+CRGE VLGGL++GIN++HI ++SKMVE  +GL+VQPHKAIVGANAF HESGIHQ
Sbjct: 319 VVMALKCRGEQVLGGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQ 378

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHK TYEIISPEDIG  R+N++GIV GKLSG    K ++ ELGYE++ + LD++FW
Sbjct: 379 DGMLKHKDTYEIISPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFW 438

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAE+KK++TD DL AL+SDEVFQP  VW+L ++QVTCG+LGLSTATVKL+DADG+E
Sbjct: 439 RFKSVAEKKKKITDDDLVALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGRE 498

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI+C+VGTGPVD+AYKAVD IVK PVTLLEYSMNAVT+GIDAIA+TRV+IRGEN +TST+
Sbjct: 499 HISCSVGTGPVDAAYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTH 558

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIKALTTEPAQLV 311
           A+TGE V RTFSG GA MDIV SSV+AY+GALNKM+ F+ +  K    E + ++
Sbjct: 559 ALTGETVHRTFSGTGADMDIVISSVRAYVGALNKMMSFRKLMAKNNKPESSAVI 612

BLAST of Moc08g35890 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 8.6e-115
Identity = 202/279 (72.40%), Postives = 243/279 (87.10%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMAL+CRGE VLGGL++GIN++HI  +SKMVEEY+GL VQPHKAIVGANAFAHESGIHQ
Sbjct: 296 VVMALKCRGEQVLGGLYTGINTQHIVPSSKMVEEYSGLQVQPHKAIVGANAFAHESGIHQ 355

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHK TYEIISP+D+G  RSN+AGIVLGKLSGRHALKS++ ELGY++D + L+++FW
Sbjct: 356 DGMLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSGRHALKSKMLELGYDIDGKELEDLFW 415

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAE+KK++TD DL AL+SDEV QP+V WKL D+Q+ CG+LGLSTATVKL++ DG+E
Sbjct: 416 RFKSVAEKKKKITDDDLIALMSDEVLQPNVYWKLGDVQIMCGSLGLSTATVKLINTDGQE 475

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI C+VGTGPVD+AYKAVD IVK P+TLLEYSMNAVTEGIDAIA+TRV I   + +T  N
Sbjct: 476 HIACSVGTGPVDAAYKAVDLIVKVPITLLEYSMNAVTEGIDAIASTRVSICSIDRHTIMN 535

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQ 296
             TG+ + RTFSG GA MD+V SSV+AYIGALNKML ++
Sbjct: 536 GSTGQTIHRTFSGTGADMDVVISSVRAYIGALNKMLSYE 574

BLAST of Moc08g35890 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.1e-96
Identity = 174/276 (63.04%), Postives = 219/276 (79.35%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMAL  +G+H L GL++ IN+RHI  TSKMVEEY+G+++QPHK +VGANAF H SGIHQ
Sbjct: 281 VVMALASKGDHALNGLYTRINTRHILETSKMVEEYSGMHLQPHKPLVGANAFVHASGIHQ 340

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYE ISPE+IG++R+   GIVLGKLSG  AL+ RL+ELGY+L  + +D++FW
Sbjct: 341 DGMLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVFW 400

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           +FK +AE+KK VTD DL+ALVS + F    +WKL DLQVTCGT+GLSTATVKL++ DG  
Sbjct: 401 QFKAMAEKKKVVTDVDLKALVSYKAFHAESIWKLGDLQVTCGTIGLSTATVKLVNIDGST 460

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H+ C++G G VDS YKA++ IVKEP  LL+YS+N+VTEGI    T RVVI  EN++TST 
Sbjct: 461 HVACSIGIGAVDSTYKAINLIVKEPTKLLDYSLNSVTEGIGVNVTARVVICRENNHTSTY 520

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKML 293
           A T +A   TFSGI A MD+V S+VKAY+ ALNK+L
Sbjct: 521 AFTEDANYPTFSGIAAEMDVVVSTVKAYLVALNKLL 556

BLAST of Moc08g35890 vs. ExPASy TrEMBL
Match: A0A6J1CL37 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.8e-153
Identity = 278/284 (97.89%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ
Sbjct: 330 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 389

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW
Sbjct: 390 DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 449

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL+ADGKE
Sbjct: 450 RFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKE 509

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H NCAVGTGPVDSAYKAVDGIVKEPV LLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN
Sbjct: 510 HTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 569

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQGVDIK
Sbjct: 570 AITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 613

BLAST of Moc08g35890 vs. ExPASy TrEMBL
Match: A0A6J1CKJ3 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.8e-153
Identity = 278/284 (97.89%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ
Sbjct: 330 VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 389

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW
Sbjct: 390 DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 449

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLL+ADGKE
Sbjct: 450 RFKAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKE 509

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H NCAVGTGPVDSAYKAVDGIVKEPV LLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN
Sbjct: 510 HTNCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 569

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQGVDIK
Sbjct: 570 AITGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGVDIK 613

BLAST of Moc08g35890 vs. ExPASy TrEMBL
Match: A0A0A0L7C8 (2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.3e-145
Identity = 261/284 (91.90%), Postives = 274/284 (96.48%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLH+GINSRHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQ
Sbjct: 341 VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 400

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKS L ELGYELD ENLDN+FW
Sbjct: 401 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFW 460

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLD+QVTCGTLGLSTATVKLLDADGKE
Sbjct: 461 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKE 520

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI C+VGTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ SYTSTN
Sbjct: 521 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 580

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQG+DIK
Sbjct: 581 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 624

BLAST of Moc08g35890 vs. ExPASy TrEMBL
Match: A0A1S3C4S6 (2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.3e-145
Identity = 265/295 (89.83%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQ
Sbjct: 343 VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 402

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+ENL N+FW
Sbjct: 403 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFW 462

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLLDADGKE
Sbjct: 463 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKE 522

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI C+VGTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ SYTSTN
Sbjct: 523 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 582

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIKALTTEPAQLVK 312
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQG+DIK  T E   L+K
Sbjct: 583 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKE-TKEKHYLLK 636

BLAST of Moc08g35890 vs. ExPASy TrEMBL
Match: A0A5D3BP53 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00350 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.1e-144
Identity = 261/284 (91.90%), Postives = 274/284 (96.48%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMALQCRGEHVLGGLH+GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQ
Sbjct: 340 VVMALQCRGEHVLGGLHTGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 399

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI++PEDIGYERSNDAGIVLGKLSGRHALKSRL ELGYELD+ENL N+FW
Sbjct: 400 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFW 459

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADLRALVSDEVFQP+V+WKLLDLQVTCGTLGLSTATVKLLDADGKE
Sbjct: 460 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKE 519

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           HI C+VGTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+ SYTSTN
Sbjct: 520 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 579

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQGVDIK 301
           A+TGEAVQRTFSGIGAGMDIV SSVKAYIGALNKMLGFQG+DIK
Sbjct: 580 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 623

BLAST of Moc08g35890 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 471.5 bits (1212), Expect = 5.5e-133
Identity = 230/279 (82.44%), Postives = 258/279 (92.47%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQ
Sbjct: 335 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQ 394

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+FW
Sbjct: 395 DGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFW 454

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDADL ALVSDEVFQP  VWKLLD+Q+TCGTLGLST+TVKL D+DGKE
Sbjct: 455 RFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKE 514

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H+ C+VGTGPVD+AYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG+N+Y+STN
Sbjct: 515 HVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTN 574

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQ 296
           A+TGE+V+RTFSG GAGMDIV SSVKAY+GALNKMLGF+
Sbjct: 575 AVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

BLAST of Moc08g35890 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 471.1 bits (1211), Expect = 7.1e-133
Identity = 231/279 (82.80%), Postives = 254/279 (91.04%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMA++CRG+HVLGGL +GI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQ
Sbjct: 337 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQ 396

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DGMLKHKGTYEII PE+IG ERSNDAGIVLGKLSGRHALK RL ELGY+LD+E L  IFW
Sbjct: 397 DGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQLSTIFW 456

Query: 137 RFKEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKE 196
           RFK VAEQKKRVTDAD+ ALVSDEVFQP  VWKLLD+Q+TCGTLGLSTATVKL DADGKE
Sbjct: 457 RFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKE 516

Query: 197 HINCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTN 256
           H+ C++GTGPVDSAYKAVD IVKEP TLLEYSMNAVTEGIDAIATTRV+IRG N Y+STN
Sbjct: 517 HVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTN 576

Query: 257 AMTGEAVQRTFSGIGAGMDIVESSVKAYIGALNKMLGFQ 296
           A+TGE VQRTFSG GAGMDIV SSVKAY+GALNKM+ F+
Sbjct: 577 AITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFK 615

BLAST of Moc08g35890 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 196.8 bits (499), Expect = 2.6e-50
Identity = 94/147 (63.95%), Postives = 123/147 (83.67%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMAL+CRG +V+ G+++ I++R I  TSKMV+EYTGL VQ HK IVGAN F HESGIHQ
Sbjct: 334 VVMALKCRGAYVINGVYTKIDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQ 393

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DG+LK++ TYEI+SPEDIG  +S ++G+VLGKLSGRHA+K RL+ELGYELD+E L+ +F 
Sbjct: 394 DGILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFS 453

Query: 137 RFKEVAEQKKRVTDADLRALV--SDEV 162
            F+++ + KKR+TDADL+ALV  SDE+
Sbjct: 454 LFRDLTKNKKRITDADLKALVTSSDEI 480

BLAST of Moc08g35890 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 193.7 bits (491), Expect = 2.2e-49
Identity = 89/141 (63.12%), Postives = 119/141 (84.40%), Query Frame = 0

Query: 17  VVMALQCRGEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 76
           VVMAL+CRGE ++ G+++ I+SR I  TSKMV+E+TG+ VQPHK IVG N F HESGIHQ
Sbjct: 334 VVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHESGIHQ 393

Query: 77  DGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFW 136
           DG+LK++ TYEI+SPED+G  +S ++GIVLGKLSGRHA+K RL+ELGYE+ +E  ++IF 
Sbjct: 394 DGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFNDIFS 453

Query: 137 RFKEVAEQKKRVTDADLRALV 158
           R++E+ + KKR+TDADL+ALV
Sbjct: 454 RYRELTKDKKRITDADLKALV 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141882.19.9e-15397.892-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia... [more]
XP_022141881.19.9e-15397.892-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia... [more]
XP_038875433.12.4e-14693.312-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida][more]
XP_008457079.11.3e-14489.83PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis melo][more]
XP_011651428.11.3e-14491.902-isopropylmalate synthase 2, chloroplastic [Cucumis sativus] >KGN57885.1 hypoth... [more]
Match NameE-valueIdentityDescription
Q9C5507.7e-13282.442-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q9LPR41.0e-13182.802-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I... [more]
O049747.0e-11770.412-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1[more]
O049738.6e-11572.402-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1[more]
Q398913.1e-9663.04Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CL374.8e-15397.892-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S... [more]
A0A6J1CKJ34.8e-15397.892-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S... [more]
A0A0A0L7C86.3e-14591.902-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1[more]
A0A1S3C4S66.3e-14589.832-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1[more]
A0A5D3BP531.1e-14491.902-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
Match NameE-valueIdentityDescription
AT1G74040.15.5e-13382.442-isopropylmalate synthase 1 [more]
AT1G18500.17.1e-13382.80methylthioalkylmalate synthase-like 4 [more]
AT5G23010.12.6e-5063.95methylthioalkylmalate synthase 1 [more]
AT5G23020.12.2e-4963.122-isopropylmalate synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0137092-isopropylmalate synthase LeuA, allosteric (dimerisation) domainSMARTSM00917LeuA_dimer_2coord: 147..292
e-value: 9.3E-29
score: 111.5
IPR0137092-isopropylmalate synthase LeuA, allosteric (dimerisation) domainPFAMPF08502LeuA_dimercoord: 148..292
e-value: 1.6E-36
score: 125.1
NoneNo IPR availableGENE3D1.10.238.260coord: 55..168
e-value: 1.8E-38
score: 133.3
NoneNo IPR availablePANTHERPTHR10277:SF652-ISOPROPYLMALATE SYNTHASE A-LIKE ISOFORM X1coord: 16..296
NoneNo IPR availablePANTHERPTHR10277HOMOCITRATE SYNTHASE-RELATEDcoord: 16..296
IPR0362302-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamilyGENE3D3.30.160.270coord: 176..292
e-value: 1.1E-24
score: 88.7
IPR0362302-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamilySUPERFAMILY1109212-isopropylmalate synthase LeuA, allosteric (dimerisation) domaincoord: 171..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g35890.1Moc08g35890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009098 leucine biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003852 2-isopropylmalate synthase activity