Moc08g35790 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g35790
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRING-type E3 ubiquitin transferase
Locationchr8: 26385527 .. 26387980 (+)
RNA-Seq ExpressionMoc08g35790
SyntenyMoc08g35790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGAAGAGCGAGACGATGAACCTCATGCTCACTGATCCCCAGCTTCTTGATTGCTATATTTGCTTTGAACCCTTGTCCGCGCCTCTCTTTCAGGTAAAATTAGCCTCTCTCTGTTTCGATTTGGATCTGATGTTCTTTTGTTCATCATCCACTCTCGAGATTTCGATCAATTTTTTGGATTATGATTACAAATGAAGTTGGGTTGGTTCTGTTGGGCCGATTTGTGAACTGGGTTGCCAGATATCATCGAGTTATGTGTAGATTATTGACTTTTTGAAGTTCTTAAAAGTTAAAAGATGGAGATTATTCTGGACATGATTGTGGGTTTGTGACGTAGTCAAGAGATTTTTGATATTCAAATAAAATCTTAAGTTGAACCCTTTGTTTTTGTTTTGCTTTCAACTCGAATTATATATAGGTTACAGACTCTTTGAAGGTGGAGATTATTCTGGACATGATTTTTAGTTTGTGATGTAATCAACAGATTATGGATCTTCAAATGAAATCTTAAGTTGAACCCTTTGCTTTTTGGTTTTGCTTTCAACTCGAATTATGTATAGATTACAGACTCTTTGGAGTTCTTAGAAGGTGGAGATTATTCTGGACATGATTGTGGGTTTGTGATATAAACAAGAGATTGTTGAAACTTGAAATTCAAATGAAGTCTTGGGTTGAATTTGAAGTCCTCTAAATCGCCTTTCTCTATGTTGTTTTTTTTTTCTTTTAATCTTCCTTGTTTCTAATTTTTATCCTTCTTCTTCTTCTCTCTCCTGCTTGTTCTCCTATTTTCTAATTTAACTAGGTAAATGAAGAATTTAATCTTAAGGTTTGAGTTTAGTTTCAATTTGATCCCATAGTTTGAACTTAGTTTCAATTTAGTTAAACTTTACAAATCGTTACAAGTGGTTACTGTATTGAACGCATGACATGCTATCTATTTATGTGGCAAATGAGACGACGTTGTCATTTTTCTTTTTTGAGTACATCAACATGGGGAGTGGCGATTCGAACCTACAATCTTTTGGTCAGAGATATATGCTTTAATCAATTGAGCTATGTTCAGGTTGACGATGACATTTTCTCTTTGGTTGGCTCTGTTAGTTAATAATATTAGGGTATAAAACTAGTAAAAGAGAAGTGAAAAAGATTGTGAGTTTTCTTGATACGTAGAAAAGGGTTCAGAATAATTTTGAGAAGTTTAATAATTATGGGATCAAATTGAAACTTTTGAAATCATATAGGTCAGATTGAAACTAATTCTAAATGGAGTCTGTGGGGTGAGCAAGTTGAAACTAAGTCCAAATTATTGGACCAAATTAATATAAGCTCAAACCACAGGACCAAATTAATAACTTGAGCCTTTTTAACTACCACAATTCACCTGCCTCTGTTACTTGATAGGATACATTTTCTTTGCCTTCTTCACCTGCCTGAGATTATTCTGGACATTGATACAGTCAGTAAATTGTTGAAATTGAATGAAATCTTCTTAAGCTGAATGGTTTATCTTGTTACTTTATCTCAAAGCATATGTCTGAACAAAAACATCTTTTTTTTTTGTTTCTTTTAATATATATTTTCTTTACCTTTCTATGTCAAACTTGGGGTTGAATTGTGGTTAATATATATGCTGTGAATATTTTTTATGGATTATAGGATTTTCTTCATTGTTTAGAAACAAGAACTTGATGAACATGCCCATTCTAGCATTTTTATATGTATAATGTTGTCTTATTGGCTATTCATATCATCCACTTTGGTATTGAAATGTTGTTTGTTTGGATGAATTAGTGTAAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACGAAAGTTCACAACAACTGCTCAAGCTGTGGCTCGGAAATTGGCCATATGCGTTGCAGACCAATCGAAAAGCTTTTGGAGTCGATCAAGATACAGTGTCGAAACTCAACATACGGCTGCAAAAAGATGATATATTGGAACAAGATAAATGACCACAAGTCATCGTGCTTGTATGAACCATGTTCATGCCCTTCATCCGATTGCTCGTTTGTTGGTTCATCAGGGCACTTGGCCTCGCATTTCATCAAGAAGCACAAAAACTCAGCCCACAGCTTCTCATATGATTCCAAGTTCACAATGTGCTTCAACCCTGGTGAAACTTATCGTATTCTTCGAGAAGAGAAAGATGGAACTCTGTACATCCTCTCTTATACGTCCGAACCTTTTGGGAACGTGGCAACTGTGAGTCGGATTGGACGTTCGTGGTCGAAGAGAAAGTTTCACTATGAAATCAAAGCAAGAGCTGAGGAGTGTGTTCTTCGTTCGCAATCAATAGCGAAGGAGATCCAAGCTCGGGTTGAGGTTCCCCCTCCTTCAACAGGATCCCTTTTGATTCCACAGGACTTCTTTAAATCTTCATCCCATCCGACCTTGGAGTTACGCATCTGGCCTGCAAGCTAA

mRNA sequence

ATGGCGGAGAAGAGCGAGACGATGAACCTCATGCTCACTGATCCCCAGCTTCTTGATTGCTATATTTGCTTTGAACCCTTGTCCGCGCCTCTCTTTCAGTGTAAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACGAAAGTTCACAACAACTGCTCAAGCTGTGGCTCGGAAATTGGCCATATGCGTTGCAGACCAATCGAAAAGCTTTTGGAGTCGATCAAGATACAGTGTCGAAACTCAACATACGGCTGCAAAAAGATGATATATTGGAACAAGATAAATGACCACAAGTCATCGTGCTTGTATGAACCATGTTCATGCCCTTCATCCGATTGCTCGTTTGTTGGTTCATCAGGGCACTTGGCCTCGCATTTCATCAAGAAGCACAAAAACTCAGCCCACAGCTTCTCATATGATTCCAAGTTCACAATGTGCTTCAACCCTGGTGAAACTTATCGTATTCTTCGAGAAGAGAAAGATGGAACTCTGTACATCCTCTCTTATACGTCCGAACCTTTTGGGAACGTGGCAACTGTGAGTCGGATTGGACGTTCGTGGTCGAAGAGAAAGTTTCACTATGAAATCAAAGCAAGAGCTGAGGAGTGTGTTCTTCGTTCGCAATCAATAGCGAAGGAGATCCAAGCTCGGGTTGAGGTTCCCCCTCCTTCAACAGGATCCCTTTTGATTCCACAGGACTTCTTTAAATCTTCATCCCATCCGACCTTGGAGTTACGCATCTGGCCTGCAAGCTAA

Coding sequence (CDS)

ATGGCGGAGAAGAGCGAGACGATGAACCTCATGCTCACTGATCCCCAGCTTCTTGATTGCTATATTTGCTTTGAACCCTTGTCCGCGCCTCTCTTTCAGTGTAAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACGAAAGTTCACAACAACTGCTCAAGCTGTGGCTCGGAAATTGGCCATATGCGTTGCAGACCAATCGAAAAGCTTTTGGAGTCGATCAAGATACAGTGTCGAAACTCAACATACGGCTGCAAAAAGATGATATATTGGAACAAGATAAATGACCACAAGTCATCGTGCTTGTATGAACCATGTTCATGCCCTTCATCCGATTGCTCGTTTGTTGGTTCATCAGGGCACTTGGCCTCGCATTTCATCAAGAAGCACAAAAACTCAGCCCACAGCTTCTCATATGATTCCAAGTTCACAATGTGCTTCAACCCTGGTGAAACTTATCGTATTCTTCGAGAAGAGAAAGATGGAACTCTGTACATCCTCTCTTATACGTCCGAACCTTTTGGGAACGTGGCAACTGTGAGTCGGATTGGACGTTCGTGGTCGAAGAGAAAGTTTCACTATGAAATCAAAGCAAGAGCTGAGGAGTGTGTTCTTCGTTCGCAATCAATAGCGAAGGAGATCCAAGCTCGGGTTGAGGTTCCCCCTCCTTCAACAGGATCCCTTTTGATTCCACAGGACTTCTTTAAATCTTCATCCCATCCGACCTTGGAGTTACGCATCTGGCCTGCAAGCTAA

Protein sequence

MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPTLELRIWPAS
Homology
BLAST of Moc08g35790 vs. NCBI nr
Match: XP_022143328.1 (E3 ubiquitin-protein ligase SINA-like 10 isoform X3 [Momordica charantia])

HSP 1 Score: 528.9 bits (1361), Expect = 2.4e-146
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0

Query: 1   MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIG 60
           MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIG
Sbjct: 1   MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIG 60

Query: 61  HMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSG 120
           HMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSG
Sbjct: 61  HMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSG 120

Query: 121 HLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATV 180
           HLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATV
Sbjct: 121 HLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATV 180

Query: 181 SRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH 240
           SRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH
Sbjct: 181 SRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH 240

Query: 241 PTLELRIWPAS 252
           PTLELRIWPAS
Sbjct: 241 PTLELRIWPAS 251

BLAST of Moc08g35790 vs. NCBI nr
Match: XP_022143327.1 (E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Momordica charantia])

HSP 1 Score: 521.9 bits (1343), Expect = 3.0e-144
Identity = 247/249 (99.20%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM
Sbjct: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 65

Query: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122
           RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL
Sbjct: 66  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 125

Query: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 182
           ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR
Sbjct: 126 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 185

Query: 183 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 242
           IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT
Sbjct: 186 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 245

Query: 243 LELRIWPAS 252
           LELRIWPAS
Sbjct: 246 LELRIWPAS 254

BLAST of Moc08g35790 vs. NCBI nr
Match: XP_022143326.1 (putative E3 ubiquitin-protein ligase SINA-like 6 isoform X1 [Momordica charantia])

HSP 1 Score: 411.4 bits (1056), Expect = 5.7e-111
Identity = 199/247 (80.57%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG EIGHM
Sbjct: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHM 65

Query: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122
            CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVGSSG +
Sbjct: 66  XCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSGQV 125

Query: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETY-RILREEKDGTLYILSYTSEPFGNVATVS 182
            SHF KKHK+ A SFSYDSKFTMCFN GETY RIL+E+ D TLY+LSYTSEPFGN ATVS
Sbjct: 126 FSHFTKKHKSPALSFSYDSKFTMCFNTGETYHRILQEKVDETLYLLSYTSEPFGNAATVS 185

Query: 183 RIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHP 242
           RIGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+SSS  
Sbjct: 186 RIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQ 245

Query: 243 TLELRIW 249
           TLE+RIW
Sbjct: 246 TLEIRIW 252

BLAST of Moc08g35790 vs. NCBI nr
Match: XP_022143329.1 (putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 [Momordica charantia])

HSP 1 Score: 342.0 bits (876), Expect = 4.2e-90
Identity = 171/246 (69.51%), Postives = 185/246 (75.20%), Query Frame = 0

Query: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG EIGHM
Sbjct: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHM 65

Query: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122
            CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVGSSG  
Sbjct: 66  XCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSG-- 125

Query: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 182
                                             +E+ D TLY+LSYTSEPFGN ATVSR
Sbjct: 126 ----------------------------------QEKVDETLYLLSYTSEPFGNAATVSR 185

Query: 183 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 242
           IGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+SSS  T
Sbjct: 186 IGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQT 215

Query: 243 LELRIW 249
           LE+RIW
Sbjct: 246 LEIRIW 215

BLAST of Moc08g35790 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 338.2 bits (866), Expect = 6.1e-89
Identity = 164/247 (66.40%), Postives = 192/247 (77.73%), Query Frame = 0

Query: 4   KSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMR 63
           K+E MN++ +DPQLLDCYIC EPLSAP+FQC+NGHI+CSSCCTKV N C SC   IG++R
Sbjct: 22  KTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIR 81

Query: 64  CRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLA 123
           CR IEK+LESIKI C NS YGCK  +  N+ NDH S C YEPCSCP SDC FVGSS  L 
Sbjct: 82  CRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLC 141

Query: 124 SHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRI 183
           SHF KKHKNSA SFSY+S+FT+C N G++YRIL+ EKDG L+ LS+T E FGN  TVSRI
Sbjct: 142 SHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRI 201

Query: 184 GRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPTL 243
           G S S++KF YEIKA+ +  +L  QSIAKEIQ RVEV PPS  SLLIP +FF SS+   L
Sbjct: 202 GPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV-PPSNRSLLIPNEFFGSSAQTIL 261

Query: 244 ELRIWPA 251
           E RIWP+
Sbjct: 262 EFRIWPS 267

BLAST of Moc08g35790 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.5e-39
Identity = 85/248 (34.27%), Postives = 135/248 (54.44%), Query Frame = 0

Query: 2   AEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGH 61
           + K   +++ L DP +LDC IC EPL  P+FQC NGH++C+ CCTKV N C SC   IG+
Sbjct: 95  SSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGY 154

Query: 62  MRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGH 121
           +RCR +EK++E+ ++ C N+ YGCK+   +     H+  C++ PCSCP  DC + G    
Sbjct: 155 VRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYYKD 214

Query: 122 LASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVS 181
           L +H   +HK+   SF ++++ T+  +  +   IL+EE DG + ++           +VS
Sbjct: 215 LNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVVQVFRALHAVYVSVS 274

Query: 182 RIG-RSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH 241
            I   +    +    +     + +L+   + K IQ +V    P  G +LIP   F  S +
Sbjct: 275 CIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQ-KVTNEHPEDGFMLIPSYLF--SGN 334

Query: 242 PTLELRIW 249
             L L+IW
Sbjct: 335 DNLNLQIW 339

BLAST of Moc08g35790 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 4.0e-35
Identity = 81/240 (33.75%), Postives = 126/240 (52.50%), Query Frame = 0

Query: 11  MLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRCRPIEKL 70
           ML D ++LDC IC+E  + P+FQC NGH++CSSCC K++N C +C S +GH RCR +E +
Sbjct: 42  MLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGHNRCRAMESV 101

Query: 71  LESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLASHFIKKH 130
           LESI I C N+  GCKK + + K   H+  C++  C+CP+ DC++  S   L +H+   H
Sbjct: 102 LESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKDLYTHYRITH 161

Query: 131 K--NSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRIGRSWS 190
              N  ++F  D   ++  N  +   I  E     L+ +    EP+G   TVS I  S  
Sbjct: 162 MEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPSSP 221

Query: 191 K-RKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPTLELRI 248
           +  ++ Y +    +   +  QS   +   ++    P    +LIP    +      LE+RI
Sbjct: 222 ELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLIPNSLLRGD---VLEMRI 278

BLAST of Moc08g35790 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 7.6e-34
Identity = 81/230 (35.22%), Postives = 124/230 (53.91%), Query Frame = 0

Query: 6   ETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRCR 65
           +T + MLTD  +LDC IC++ L  P+FQC NGH++CSSCC K+ N C +C   +GH+RCR
Sbjct: 31  KTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCR 90

Query: 66  PIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLASH 125
            +E++LES+ + CR +  GC K IY+ + + H+  C + PCSCP   C++ GS   L  H
Sbjct: 91  AMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEH 150

Query: 126 FIKKHK--NSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRI 185
           +   H   ++A+SF+  S         +   I R  +   L+++    EP G   +VS I
Sbjct: 151 YDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCI 210

Query: 186 GRSWSK-RKFHYEIKARAEECV-LRSQSIAKEIQARVEVPPPSTGSLLIP 232
             S  +  +F Y +     E V +  QS   +   +V    P    +LIP
Sbjct: 211 APSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIP 260

BLAST of Moc08g35790 vs. ExPASy Swiss-Prot
Match: Q9C9M0 (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.3e-33
Identity = 86/251 (34.26%), Postives = 129/251 (51.39%), Query Frame = 0

Query: 5   SETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRC 64
           S   ++ L +  +L+C  CF+PL  P+FQC NGH++C  CC K+   CS C   IG +RC
Sbjct: 71  SSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRC 130

Query: 65  RPIEKLLESIKIQCRNSTYGCKK-MIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLA 124
           R +EK++++  + C N+ YGCK+   Y N++  H+  C++ PCSCP  DC+++G    L 
Sbjct: 131 RAMEKVIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLI 190

Query: 125 SHFIKKHKNS---AHSFSYDSK--FTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVA 184
           +HF   HK S    +SF +D    F +  +  +   I  EEK G L+++       G  A
Sbjct: 191 NHFRATHKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYA 250

Query: 185 TVSRIGRSWSK-RKFHYEIKARAEECVLRSQSIAKEIQ-ARVEVPPPSTGSLLIPQDFFK 244
           TVS I     + RKF   +        LR     K IQ  R +   P    LLIP  +  
Sbjct: 251 TVSHIAPMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIP-SYMV 310

Query: 245 SSSHPTLELRI 248
           +  H  +E+ I
Sbjct: 311 NGDHMKMEISI 320

BLAST of Moc08g35790 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.9e-32
Identity = 79/250 (31.60%), Postives = 124/250 (49.60%), Query Frame = 0

Query: 4   KSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMR 63
           K +   ++L D  +LDC IC E L++P+FQC NGH++C SCC K+ N C +C   +GH R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 64  CRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLA 123
            R +E +LESI I C N  +GC K  ++ K + H+  C++  CSCPSS C + GS   L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 124 SHFIKKHKN----SAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVAT 183
           +H+   H      +   F   + FT      +   I R  +   L  +    EP G   T
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 184 VSRIGRSWSK-RKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSS 243
           VS I  S  +  +F Y++    +   +  +S   +   +V +  P    +LIP    +  
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPHSLLRGD 261

Query: 244 SHPTLELRIW 249
               LE++++
Sbjct: 262 ---LLEMQVF 268

BLAST of Moc08g35790 vs. ExPASy TrEMBL
Match: A0A6J1CQF8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013227 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.2e-146
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0

Query: 1   MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIG 60
           MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIG
Sbjct: 1   MAEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIG 60

Query: 61  HMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSG 120
           HMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSG
Sbjct: 61  HMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSG 120

Query: 121 HLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATV 180
           HLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATV
Sbjct: 121 HLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATV 180

Query: 181 SRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH 240
           SRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH
Sbjct: 181 SRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH 240

Query: 241 PTLELRIWPAS 252
           PTLELRIWPAS
Sbjct: 241 PTLELRIWPAS 251

BLAST of Moc08g35790 vs. ExPASy TrEMBL
Match: A0A6J1CQH1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013227 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.4e-144
Identity = 247/249 (99.20%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM
Sbjct: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 65

Query: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122
           RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL
Sbjct: 66  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 125

Query: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 182
           ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR
Sbjct: 126 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 185

Query: 183 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 242
           IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT
Sbjct: 186 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 245

Query: 243 LELRIWPAS 252
           LELRIWPAS
Sbjct: 246 LELRIWPAS 254

BLAST of Moc08g35790 vs. ExPASy TrEMBL
Match: A0A6J1CPY7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013227 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.7e-111
Identity = 199/247 (80.57%), Postives = 215/247 (87.04%), Query Frame = 0

Query: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG EIGHM
Sbjct: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHM 65

Query: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122
            CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVGSSG +
Sbjct: 66  XCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSGQV 125

Query: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETY-RILREEKDGTLYILSYTSEPFGNVATVS 182
            SHF KKHK+ A SFSYDSKFTMCFN GETY RIL+E+ D TLY+LSYTSEPFGN ATVS
Sbjct: 126 FSHFTKKHKSPALSFSYDSKFTMCFNTGETYHRILQEKVDETLYLLSYTSEPFGNAATVS 185

Query: 183 RIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHP 242
           RIGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+SSS  
Sbjct: 186 RIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQ 245

Query: 243 TLELRIW 249
           TLE+RIW
Sbjct: 246 TLEIRIW 252

BLAST of Moc08g35790 vs. ExPASy TrEMBL
Match: A0A6J1CP04 (putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111013227 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 2.0e-90
Identity = 171/246 (69.51%), Postives = 185/246 (75.20%), Query Frame = 0

Query: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG EIGHM
Sbjct: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHM 65

Query: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122
            CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVGSSG  
Sbjct: 66  XCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSG-- 125

Query: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 182
                                             +E+ D TLY+LSYTSEPFGN ATVSR
Sbjct: 126 ----------------------------------QEKVDETLYLLSYTSEPFGNAATVSR 185

Query: 183 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 242
           IGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+SSS  T
Sbjct: 186 IGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQT 215

Query: 243 LELRIW 249
           LE+RIW
Sbjct: 246 LEIRIW 215

BLAST of Moc08g35790 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 3.0e-89
Identity = 164/247 (66.40%), Postives = 192/247 (77.73%), Query Frame = 0

Query: 4   KSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMR 63
           K+E MN++ +DPQLLDCYIC EPLSAP+FQC+NGHI+CSSCCTKV N C SC   IG++R
Sbjct: 22  KTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIR 81

Query: 64  CRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLA 123
           CR IEK+LESIKI C NS YGCK  +  N+ NDH S C YEPCSCP SDC FVGSS  L 
Sbjct: 82  CRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLC 141

Query: 124 SHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRI 183
           SHF KKHKNSA SFSY+S+FT+C N G++YRIL+ EKDG L+ LS+T E FGN  TVSRI
Sbjct: 142 SHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRI 201

Query: 184 GRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPTL 243
           G S S++KF YEIKA+ +  +L  QSIAKEIQ RVEV PPS  SLLIP +FF SS+   L
Sbjct: 202 GPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV-PPSNRSLLIPNEFFGSSAQTIL 261

Query: 244 ELRIWPA 251
           E RIWP+
Sbjct: 262 EFRIWPS 267

BLAST of Moc08g35790 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 163.3 bits (412), Expect = 2.5e-40
Identity = 85/248 (34.27%), Postives = 135/248 (54.44%), Query Frame = 0

Query: 2   AEKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGH 61
           + K   +++ L DP +LDC IC EPL  P+FQC NGH++C+ CCTKV N C SC   IG+
Sbjct: 95  SSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGY 154

Query: 62  MRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGH 121
           +RCR +EK++E+ ++ C N+ YGCK+   +     H+  C++ PCSCP  DC + G    
Sbjct: 155 VRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTGYYKD 214

Query: 122 LASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVS 181
           L +H   +HK+   SF ++++ T+  +  +   IL+EE DG + ++           +VS
Sbjct: 215 LNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTILQEENDGHVIVVQVFRALHAVYVSVS 274

Query: 182 RIG-RSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSH 241
            I   +    +    +     + +L+   + K IQ +V    P  G +LIP   F  S +
Sbjct: 275 CIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQ-KVTNEHPEDGFMLIPSYLF--SGN 334

Query: 242 PTLELRIW 249
             L L+IW
Sbjct: 335 DNLNLQIW 339

BLAST of Moc08g35790 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 149.8 bits (377), Expect = 2.9e-36
Identity = 81/240 (33.75%), Postives = 126/240 (52.50%), Query Frame = 0

Query: 11  MLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRCRPIEKL 70
           ML D ++LDC IC+E  + P+FQC NGH++CSSCC K++N C +C S +GH RCR +E +
Sbjct: 42  MLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGHNRCRAMESV 101

Query: 71  LESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLASHFIKKH 130
           LESI I C N+  GCKK + + K   H+  C++  C+CP+ DC++  S   L +H+   H
Sbjct: 102 LESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKDLYTHYRITH 161

Query: 131 K--NSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRIGRSWS 190
              N  ++F  D   ++  N  +   I  E     L+ +    EP+G   TVS I  S  
Sbjct: 162 MEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPSSP 221

Query: 191 K-RKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPTLELRI 248
           +  ++ Y +    +   +  QS   +   ++    P    +LIP    +      LE+RI
Sbjct: 222 ELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLIPNSLLRGD---VLEMRI 278

BLAST of Moc08g35790 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 145.6 bits (366), Expect = 5.4e-35
Identity = 81/230 (35.22%), Postives = 124/230 (53.91%), Query Frame = 0

Query: 6   ETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRCR 65
           +T + MLTD  +LDC IC++ L  P+FQC NGH++CSSCC K+ N C +C   +GH+RCR
Sbjct: 31  KTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPVGHIRCR 90

Query: 66  PIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLASH 125
            +E++LES+ + CR +  GC K IY+ + + H+  C + PCSCP   C++ GS   L  H
Sbjct: 91  AMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGSYKDLYEH 150

Query: 126 FIKKHK--NSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSRI 185
           +   H   ++A+SF+  S         +   I R  +   L+++    EP G   +VS I
Sbjct: 151 YDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVYEKKLLFVVQCFEEPCGVYVSVSCI 210

Query: 186 GRSWSK-RKFHYEIKARAEECV-LRSQSIAKEIQARVEVPPPSTGSLLIP 232
             S  +  +F Y +     E V +  QS   +   +V    P    +LIP
Sbjct: 211 APSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLKVSSQRPKDSFMLIP 260

BLAST of Moc08g35790 vs. TAIR 10
Match: AT1G66650.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 144.8 bits (364), Expect = 9.2e-35
Identity = 86/251 (34.26%), Postives = 129/251 (51.39%), Query Frame = 0

Query: 5   SETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMRC 64
           S   ++ L +  +L+C  CF+PL  P+FQC NGH++C  CC K+   CS C   IG +RC
Sbjct: 71  SSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRC 130

Query: 65  RPIEKLLESIKIQCRNSTYGCKK-MIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLA 124
           R +EK++++  + C N+ YGCK+   Y N++  H+  C++ PCSCP  DC+++G    L 
Sbjct: 131 RAMEKVIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLI 190

Query: 125 SHFIKKHKNS---AHSFSYDSK--FTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVA 184
           +HF   HK S    +SF +D    F +  +  +   I  EEK G L+++       G  A
Sbjct: 191 NHFRATHKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYA 250

Query: 185 TVSRIGRSWSK-RKFHYEIKARAEECVLRSQSIAKEIQ-ARVEVPPPSTGSLLIPQDFFK 244
           TVS I     + RKF   +        LR     K IQ  R +   P    LLIP  +  
Sbjct: 251 TVSHIAPMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIP-SYMV 310

Query: 245 SSSHPTLELRI 248
           +  H  +E+ I
Sbjct: 311 NGDHMKMEISI 320

BLAST of Moc08g35790 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-33
Identity = 79/250 (31.60%), Postives = 124/250 (49.60%), Query Frame = 0

Query: 4   KSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHMR 63
           K +   ++L D  +LDC IC E L++P+FQC NGH++C SCC K+ N C +C   +GH R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 64  CRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHLA 123
            R +E +LESI I C N  +GC K  ++ K + H+  C++  CSCPSS C + GS   L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 124 SHFIKKHKN----SAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVAT 183
           +H+   H      +   F   + FT      +   I R  +   L  +    EP G   T
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 184 VSRIGRSWSK-RKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSS 243
           VS I  S  +  +F Y++    +   +  +S   +   +V +  P    +LIP    +  
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPHSLLRGD 261

Query: 244 SHPTLELRIW 249
               LE++++
Sbjct: 262 ---LLEMQVF 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143328.12.4e-146100.00E3 ubiquitin-protein ligase SINA-like 10 isoform X3 [Momordica charantia][more]
XP_022143327.13.0e-14499.20E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Momordica charantia][more]
XP_022143326.15.7e-11180.57putative E3 ubiquitin-protein ligase SINA-like 6 isoform X1 [Momordica charantia... [more]
XP_022143329.14.2e-9069.51putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 [Momordica charantia... [more]
XP_022143325.16.1e-8966.40E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q84K343.5e-3934.27E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
Q9FKD74.0e-3533.75E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3... [more]
Q9FKD97.6e-3435.22Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702... [more]
Q9C9M01.3e-3334.26E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g6... [more]
Q9FKD51.9e-3231.60Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1CQF81.2e-146100.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322... [more]
A0A6J1CQH11.4e-14499.20RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322... [more]
A0A6J1CPY72.7e-11180.57RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322... [more]
A0A6J1CP042.0e-9069.51putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 OS=Momordica charant... [more]
A0A6J1CNI13.0e-8966.40RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322... [more]
Match NameE-valueIdentityDescription
AT5G37930.12.5e-4034.27Protein with RING/U-box and TRAF-like domains [more]
AT5G37890.12.9e-3633.75Protein with RING/U-box and TRAF-like domains [more]
AT5G37870.15.4e-3535.22Protein with RING/U-box and TRAF-like domains [more]
AT1G66650.19.2e-3534.26Protein with RING/U-box and TRAF-like domains [more]
AT5G37910.11.3e-3331.60Protein with RING/U-box and TRAF-like domains [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 68..126
e-value: 7.2E-15
score: 57.0
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 63..220
e-value: 5.1E-14
score: 52.7
IPR044286E3 ubiquitin-protein ligase SINA-like, plantPANTHERPTHR46632E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4coord: 4..249
NoneNo IPR availablePANTHERPTHR46632:SF16E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4coord: 4..249
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 73..233
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 73..131
score: 13.374914

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g35790.1Moc08g35790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016740 transferase activity
molecular_function GO:0008270 zinc ion binding