Moc08g35780 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc08g35780
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RING-type E3 ubiquitin transferase
Location	chr8: 26381584 .. 26384139 (+)
RNA-Seq Expression	Moc08g35780
Synteny	Moc08g35780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGCGAAAAGGAGAACAGCGAGACGATGAACCTCATACTCACTGATCCCCAGCTTCTTGATTGCTATATTTGCTTTGAACCCTTGTCCGCGCCTCTCTTTCAGGTAAAATTAGCCTCTCTCTGTTTCGATTTGGATCTGATGTTCTTTTGTTCATCATCCACTCTCAAGATTTGGATCCATTTTTTGGATTATGATTATAAATGAAGTTGGGTTGGTTTCCGTTGGGTCGATTTGTGAATTGTGTTGCCAGATATAATTGAGTTATGTGTGGATTATTGACTCTTTGAAGTTCTTAAAAGTTAAAAGATGGAGATTATTCTGGACATGTTTGTGGGTTTGCGATGTAGTCAAGAGATTATTGATATTCAAATAAAATCTTAAGTTGAACCGTTTGTTTTTTGTTTTTTGTTTTTGTTTTTGTTTTTTTTGCTTTCAATTCGAATTATATATGGATTTCAAACTCTTTGAAGTTCTTAAAAGGTGGAGGTTGTTCTGGACATGATTGTGGGTTTGTGATGTAATCAACAGATTATTGATATTCAAATGAAATCTTAAGTTGAGCCCTTTGTTTTTTGTTTTGGTTTTGCTTTCAACTCGAATTATATGTAGATTTCAAATAAGGTGGAGAATGGAGATTATTCTGGACATGATTGTGGCCTGTGGGTTTGTGATATAATCAAGAGATTGTTGAAACTTTAAATTCAAATGAAGTCTTAGGTTGAATTTGAAGACCTTTAAATCCCATTTCTCTGTGTTGTTTTTTTTTCACTAATCTTCTTTGTTTTTAATTTTTATTTTGCTTTCAACTAGATAGATATAATGCTTCCTTCTTCTTCTTCTCCTCTCTGCTTGTTCTCCTATTTTCTAATTTAACTAGGTTAAATGACCAATTTAGTTTTGAAGGTTGAATTTAGTTTTAATATTATCCCTGTATTTTGAACTTAGTTTCAATTTGTCAAACCTTACCAATCATTACAAGTGGTTACTATATTGAACCCATGAAATGTTATCTATTTATGTGCCAAATGAGATGACGTTTTCTTTTCCTTTTAGTACATCAACATGTGGGGTGGAGATTTGAACCTATAACCTTTATGGGGATATATATCTTAACCAATTAAACTTTTATTCAGCTCGCTTTCTGTGCTAATTATACTAGGGTATAAAATTTAGTAAAAGAGAAGTAAAAAGATTGTGAATTTTTTTGTTAAGTAAAAAAAGGTTTAGGAAAATTTGTAAGTTCAAAAATTATAGGGATTAAATTAGAACGAAATCATAGGGGTTTAGATTGAAATTAAGTCTAAATGAAGTCGTGCGGGGGCATTGAGCAAGTTGAAACTAAGTCCAAATTAGGGGGCACTGAGCAAGTTGAAACTAAGTCCAAATAAGTCCAAATTATAGGGACTGAGTTAAGATTAAGCTCAAACTATAAATTAATATCTTTAGCCTTTTTTTAACTATCACTATTCACTTGCCGCTGTTACTTGATATGATACATTTTCTTTTCCTTCTTCACCTGCCTGAGATTATTCTGGACATTGATATAGTCAAGAAATTGTTGAAATTGAAATGAAATCTTCTTTAGCTGAATGGTTTATCTTGTTACTTTACGTTATCTCAAAGCATATATCTGAACAAAAACATGTTATTTTGATATTTTCTTCACCTTTCTATGTCAAACTTGGGGTTGAATTGTTGTTAATATATATGCTGTGAATATTTTTTATGGATTATAGGATTTTCTTCATTGTTTAGAGACAAGAACATGAAAGGGATGAATGAATATGCCCATTCTTGCATTTTTATATTTATAACGTTGTCTTATTTACAATTCATATCATCCACTCTGGTGTTGAAATGTTGTTTGTTTGGATGAATTAGTGTAAGAATGGGCATATTTCTTGCTCTAGCTGCTGCATCAAAGCTCACAACCAATGCTCGAGCTGTGGCTTGGAAATTGGCCATATGAGTTGCAGACCAATCGAAAAGCTTCTGGAGTCGGTCAAGAAGCATTGTCGAAACTCAATATACGGCTGCAAAACGATGATATATTGGAACAAGATAAATGACCACGAGTCATTGTGCGTGTATGAACCATGTTCATGCCCTTTATCCAATTGCTCATTTGTGGGTTCATCCGGGCAGGTATTCTCACATTTCACCAAGAAGCACAAAAGCCCTGCCCTGAGCTTCTCATATGATTCCAAATTCACAATGTGCTTCAACACTGGTGAAACTTATCATCGTATTCTTCAGGAAAAGGTAGATGAAACTCTGTACCTCCTCTCTTACACGTCCGAACCTTTTGGGAATGCAGCAACCGTGAGTCGGATTGGACGTTCGATGTCGATGAGAAAGTTTCACTATGAAATCAAAGCAAAAGCTGAGGAGCGTGTTCTTTGTTTGCAATCAATAGCGAAGGAGATCCGAGCTCAGGTTGAGGTTACCTCTCCTTCAAATGGATCCCTTTTGATTCCAAAGGACTTCTTTGAATCTTCATCCCGGCAGACCTTGGAGATACGCATCTGGTGGCGTGCAAGCCAAAATTAG

mRNA sequence

ATGGCGGGCGAAAAGGAGAACAGCGAGACGATGAACCTCATACTCACTGATCCCCAGCTTCTTGATTGCTATATTTGCTTTGAACCCTTGTCCGCGCCTCTCTTTCAGTGTAAGAATGGGCATATTTCTTGCTCTAGCTGCTGCATCAAAGCTCACAACCAATGCTCGAGCTGTGGCTTGGAAATTGGCCATATGAGTTGCAGACCAATCGAAAAGCTTCTGGAGTCGGTCAAGAAGCATTGTCGAAACTCAATATACGGCTGCAAAACGATGATATATTGGAACAAGATAAATGACCACGAGTCATTGTGCGTGTATGAACCATGTTCATGCCCTTTATCCAATTGCTCATTTGTGGGTTCATCCGGGCAGGAAAAGGTAGATGAAACTCTGTACCTCCTCTCTTACACGTCCGAACCTTTTGGGAATGCAGCAACCGTGAGTCGGATTGGACGTTCGATGTCGATGAGAAAGTTTCACTATGAAATCAAAGCAAAAGCTGAGGAGCGTGTTCTTTGTTTGCAATCAATAGCGAAGGAGATCCGAGCTCAGGTTGAGGTTACCTCTCCTTCAAATGGATCCCTTTTGATTCCAAAGGACTTCTTTGAATCTTCATCCCGGCAGACCTTGGAGATACGCATCTGGTGGCGTGCAAGCCAAAATTAG

Coding sequence (CDS)

ATGGCGGGCGAAAAGGAGAACAGCGAGACGATGAACCTCATACTCACTGATCCCCAGCTTCTTGATTGCTATATTTGCTTTGAACCCTTGTCCGCGCCTCTCTTTCAGTGTAAGAATGGGCATATTTCTTGCTCTAGCTGCTGCATCAAAGCTCACAACCAATGCTCGAGCTGTGGCTTGGAAATTGGCCATATGAGTTGCAGACCAATCGAAAAGCTTCTGGAGTCGGTCAAGAAGCATTGTCGAAACTCAATATACGGCTGCAAAACGATGATATATTGGAACAAGATAAATGACCACGAGTCATTGTGCGTGTATGAACCATGTTCATGCCCTTTATCCAATTGCTCATTTGTGGGTTCATCCGGGCAGGAAAAGGTAGATGAAACTCTGTACCTCCTCTCTTACACGTCCGAACCTTTTGGGAATGCAGCAACCGTGAGTCGGATTGGACGTTCGATGTCGATGAGAAAGTTTCACTATGAAATCAAAGCAAAAGCTGAGGAGCGTGTTCTTTGTTTGCAATCAATAGCGAAGGAGATCCGAGCTCAGGTTGAGGTTACCTCTCCTTCAAATGGATCCCTTTTGATTCCAAAGGACTTCTTTGAATCTTCATCCCGGCAGACCTTGGAGATACGCATCTGGTGGCGTGCAAGCCAAAATTAG

Protein sequence

MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN

Homology

[Close]

BLAST of Moc08g35780 vs. NCBI nr
Match: XP_022143329.1 (putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 [Momordica charantia])

HSP 1 Score: 460.3 bits (1183), Expect = 9.4e-126
Identity = 220/221 (99.55%), Postives = 220/221 (99.55%), Query Frame = 0


Query: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60
           MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL
Sbjct: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60

Query: 61  EIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120
           EIGHM CRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG
Sbjct: 61  EIGHMXCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120

Query: 121 SSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKE 180
           SSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKE
Sbjct: 121 SSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKE 180

Query: 181 IRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN 222
           IRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN
Sbjct: 181 IRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN 221

[Close]

BLAST of Moc08g35780 vs. NCBI nr
Match: XP_022143326.1 (putative E3 ubiquitin-protein ligase SINA-like 6 isoform X1 [Momordica charantia])

HSP 1 Score: 441.8 bits (1135), Expect = 3.5e-120
Identity = 220/258 (85.27%), Postives = 220/258 (85.27%), Query Frame = 0


Query: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60
           MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL
Sbjct: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60

Query: 61  EIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120
           EIGHM CRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG
Sbjct: 61  EIGHMXCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120

Query: 121 SSG-------------------------------------QEKVDETLYLLSYTSEPFGN 180
           SSG                                     QEKVDETLYLLSYTSEPFGN
Sbjct: 121 SSGQVFSHFTKKHKSPALSFSYDSKFTMCFNTGETYHRILQEKVDETLYLLSYTSEPFGN 180

Query: 181 AATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFE 222
           AATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFE
Sbjct: 181 AATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFE 240

[Close]

BLAST of Moc08g35780 vs. NCBI nr
Match: XP_022143327.1 (E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Momordica charantia])

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-94
Identity = 178/251 (70.92%), Postives = 191/251 (76.10%), Query Frame = 0


Query: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60
           MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG 
Sbjct: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGS 60

Query: 61  EIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120
           EIGHM CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVG
Sbjct: 61  EIGHMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVG 120

Query: 121 SSG------------------------------------QEKVDETLYLLSYTSEPFGNA 180
           SSG                                    +E+ D TLY+LSYTSEPFGN 
Sbjct: 121 SSGHLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNV 180

Query: 181 ATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFES 216
           ATVSRIGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+S
Sbjct: 181 ATVSRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKS 240

[Close]

BLAST of Moc08g35780 vs. NCBI nr
Match: XP_022143328.1 (E3 ubiquitin-protein ligase SINA-like 10 isoform X3 [Momordica charantia])

HSP 1 Score: 344.0 bits (881), Expect = 9.8e-91
Identity = 171/246 (69.51%), Postives = 185/246 (75.20%), Query Frame = 0


Query: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHM 65
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG EIGHM
Sbjct: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62

Query: 66  SCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSG-- 125
            CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVGSSG  
Sbjct: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122

Query: 126 ----------------------------------QEKVDETLYLLSYTSEPFGNAATVSR 185
                                             +E+ D TLY+LSYTSEPFGN ATVSR
Sbjct: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 182

Query: 186 IGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQT 216
           IGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+SSS  T
Sbjct: 183 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 242

[Close]

BLAST of Moc08g35780 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 262.3 bits (669), Expect = 3.7e-66
Identity = 135/244 (55.33%), Postives = 161/244 (65.98%), Query Frame = 0


Query: 8   SETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSC 67
           +E MN+I +DPQLLDCYIC EPLSAP+FQC+NGHI+CSSCC K  N+C SC L IG++ C
Sbjct: 23  TEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRC 82

Query: 68  RPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSG---- 127
           R IEK+LES+K  C NSIYGCK  +  N+ NDH SLC YEPCSCP+S+C FVGSS     
Sbjct: 83  RAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCS 142

Query: 128 --------------------------------QEKVDETLYLLSYTSEPFGNAATVSRIG 187
                                           Q + D  L+ LS+T E FGNA TVSRIG
Sbjct: 143 HFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIG 202

Query: 188 RSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQTLE 216
            S S +KF YEIKAK +  +LCLQSIAKEI+ +VEV  PSN SLLIP +FF SS++  LE
Sbjct: 203 PSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV-PPSNRSLLIPNEFFGSSAQTILE 262

[Close]

BLAST of Moc08g35780 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.8e-31
Identity = 55/119 (46.22%), Postives = 80/119 (67.23%), Query Frame = 0


Query: 3   GEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEI 62
           G K   +T + +LTD  +LDC IC++ L  P+FQC NGH++CSSCC K  N+C +C L +
Sbjct: 25  GRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPV 84

Query: 63  GHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGS 122
           GH+ CR +E++LESV   CR +  GC   IY+ + + HE +C + PCSCP+  C++ GS
Sbjct: 85  GHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGS 143

[Close]

BLAST of Moc08g35780 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.9e-29
Identity = 51/110 (46.36%), Postives = 74/110 (67.27%), Query Frame = 0


Query: 11  MNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSCRPI 70
           +++ L DP +LDC IC EPL  P+FQC NGH++C+ CC K  N+C SC L IG++ CR +
Sbjct: 101 LSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAM 160

Query: 71  EKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 121
           EK++E+ +  C N+ YGCK    +     HE +CV+ PCSCP+ +C + G
Sbjct: 161 EKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTG 210

[Close]

BLAST of Moc08g35780 vs. ExPASy Swiss-Prot
Match: Q9C9M0 (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.2e-28
Identity = 50/114 (43.86%), Postives = 77/114 (67.54%), Query Frame = 0


Query: 8   SETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSC 67
           S   ++ L +  +L+C  CF+PL  P+FQC NGH++C  CCIK   +CS C L IG + C
Sbjct: 71  SSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRC 130

Query: 68  RPIEKLLESVKKHCRNSIYGCK-TMIYWNKINDHESLCVYEPCSCPLSNCSFVG 121
           R +EK++++    C N+IYGCK +  Y N++  HE +CV+ PCSCP+ +C+++G
Sbjct: 131 RAMEKVIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIG 184

[Close]

BLAST of Moc08g35780 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.7e-26
Identity = 68/249 (27.31%), Postives = 112/249 (44.98%), Query Frame = 0


Query: 5   KENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGH 64
           K +++  + +L D ++LDC IC+E  + P+FQC NGH++CSSCC K +N+C +C   +GH
Sbjct: 33  KRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGH 92

Query: 65  MSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSGQ 124
             CR +E +LES+   C N+  GCK  + + K   HE  C++  C+CP  +C++  S   
Sbjct: 93  NRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKD 152

Query: 125 --------------------------------------EKVDETLYLLSYTSEPFGNAAT 184
                                                 E +   L+ +    EP+G   T
Sbjct: 153 LYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVT 212

Query: 185 VSRIG-RSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESS 215
           VS I   S  + ++ Y +    +   +  QS   +   ++   +P    +LIP       
Sbjct: 213 VSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLIPNSLLRG- 272

[Close]

BLAST of Moc08g35780 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-25
Identity = 48/109 (44.04%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 13  LILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSCRPIEK 72
           ++L D  +LDC IC E L++P+FQC NGH++C SCC K  N+C +C L +GH   R +E 
Sbjct: 28  IVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMES 87

Query: 73  LLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGS 122
           +LES+   C N  +GC    ++ K + HE  C++  CSCP S C + GS
Sbjct: 88  VLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGS 136

[Close]

BLAST of Moc08g35780 vs. ExPASy TrEMBL
Match: A0A6J1CP04 (putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111013227 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.5e-126
Identity = 220/221 (99.55%), Postives = 220/221 (99.55%), Query Frame = 0


Query: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60
           MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL
Sbjct: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60

Query: 61  EIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120
           EIGHM CRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG
Sbjct: 61  EIGHMXCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120

Query: 121 SSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKE 180
           SSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKE
Sbjct: 121 SSGQEKVDETLYLLSYTSEPFGNAATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKE 180

Query: 181 IRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN 222
           IRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN
Sbjct: 181 IRAQVEVTSPSNGSLLIPKDFFESSSRQTLEIRIWWRASQN 221

[Close]

BLAST of Moc08g35780 vs. ExPASy TrEMBL
Match: A0A6J1CPY7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013227 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.7e-120
Identity = 220/258 (85.27%), Postives = 220/258 (85.27%), Query Frame = 0


Query: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60
           MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL
Sbjct: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60

Query: 61  EIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120
           EIGHM CRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG
Sbjct: 61  EIGHMXCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120

Query: 121 SSG-------------------------------------QEKVDETLYLLSYTSEPFGN 180
           SSG                                     QEKVDETLYLLSYTSEPFGN
Sbjct: 121 SSGQVFSHFTKKHKSPALSFSYDSKFTMCFNTGETYHRILQEKVDETLYLLSYTSEPFGN 180

Query: 181 AATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFE 222
           AATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFE
Sbjct: 181 AATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFE 240

[Close]

BLAST of Moc08g35780 vs. ExPASy TrEMBL
Match: A0A6J1CQH1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013227 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 5.4e-95
Identity = 178/251 (70.92%), Postives = 191/251 (76.10%), Query Frame = 0


Query: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGL 60
           MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG 
Sbjct: 1   MAGEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGS 60

Query: 61  EIGHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 120
           EIGHM CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVG
Sbjct: 61  EIGHMRCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVG 120

Query: 121 SSG------------------------------------QEKVDETLYLLSYTSEPFGNA 180
           SSG                                    +E+ D TLY+LSYTSEPFGN 
Sbjct: 121 SSGHLASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNV 180

Query: 181 ATVSRIGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFES 216
           ATVSRIGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+S
Sbjct: 181 ATVSRIGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKS 240

[Close]

BLAST of Moc08g35780 vs. ExPASy TrEMBL
Match: A0A6J1CQF8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013227 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.7e-91
Identity = 171/246 (69.51%), Postives = 185/246 (75.20%), Query Frame = 0


Query: 6   ENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHM 65
           E SETMNL+LTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCC K HN CSSCG EIGHM
Sbjct: 3   EKSETMNLMLTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCTKVHNNCSSCGSEIGHM 62

Query: 66  SCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSG-- 125
            CRPIEKLLES+K  CRNS YGCK MIYWNKINDH+S C+YEPCSCP S+CSFVGSSG  
Sbjct: 63  RCRPIEKLLESIKIQCRNSTYGCKKMIYWNKINDHKSSCLYEPCSCPSSDCSFVGSSGHL 122

Query: 126 ----------------------------------QEKVDETLYLLSYTSEPFGNAATVSR 185
                                             +E+ D TLY+LSYTSEPFGN ATVSR
Sbjct: 123 ASHFIKKHKNSAHSFSYDSKFTMCFNPGETYRILREEKDGTLYILSYTSEPFGNVATVSR 182

Query: 186 IGRSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQT 216
           IGRS S RKFHYEIKA+AEE VL  QSIAKEI+A+VEV  PS GSLLIP+DFF+SSS  T
Sbjct: 183 IGRSWSKRKFHYEIKARAEECVLRSQSIAKEIQARVEVPPPSTGSLLIPQDFFKSSSHPT 242

[Close]

BLAST of Moc08g35780 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.8e-66
Identity = 135/244 (55.33%), Postives = 161/244 (65.98%), Query Frame = 0


Query: 8   SETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSC 67
           +E MN+I +DPQLLDCYIC EPLSAP+FQC+NGHI+CSSCC K  N+C SC L IG++ C
Sbjct: 23  TEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIACSSCCTKVQNKCPSCSLSIGYIRC 82

Query: 68  RPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSG---- 127
           R IEK+LES+K  C NSIYGCK  +  N+ NDH SLC YEPCSCP+S+C FVGSS     
Sbjct: 83  RAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLCTYEPCSCPVSDCLFVGSSEKLCS 142

Query: 128 --------------------------------QEKVDETLYLLSYTSEPFGNAATVSRIG 187
                                           Q + D  L+ LS+T E FGNA TVSRIG
Sbjct: 143 HFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKDGALFFLSFTFEIFGNAVTVSRIG 202

Query: 188 RSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESSSRQTLE 216
            S S +KF YEIKAK +  +LCLQSIAKEI+ +VEV  PSN SLLIP +FF SS++  LE
Sbjct: 203 PSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV-PPSNRSLLIPNEFFGSSAQTILE 262

[Close]

BLAST of Moc08g35780 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-32
Identity = 55/119 (46.22%), Postives = 80/119 (67.23%), Query Frame = 0


Query: 3   GEKENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEI 62
           G K   +T + +LTD  +LDC IC++ L  P+FQC NGH++CSSCC K  N+C +C L +
Sbjct: 25  GRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLACSSCCPKLRNKCPACALPV 84

Query: 63  GHMSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGS 122
           GH+ CR +E++LESV   CR +  GC   IY+ + + HE +C + PCSCP+  C++ GS
Sbjct: 85  GHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKICNFSPCSCPVQGCNYTGS 143

[Close]

BLAST of Moc08g35780 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 130.2 bits (326), Expect = 2.1e-30
Identity = 51/110 (46.36%), Postives = 74/110 (67.27%), Query Frame = 0


Query: 11  MNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSCRPI 70
           +++ L DP +LDC IC EPL  P+FQC NGH++C+ CC K  N+C SC L IG++ CR +
Sbjct: 101 LSVTLLDPDVLDCPICCEPLKIPIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAM 160

Query: 71  EKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVG 121
           EK++E+ +  C N+ YGCK    +     HE +CV+ PCSCP+ +C + G
Sbjct: 161 EKVIEASRVSCLNAKYGCKESTSYGNRFSHEQVCVFTPCSCPILDCHYTG 210

[Close]

BLAST of Moc08g35780 vs. TAIR 10
Match: AT1G66650.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 125.6 bits (314), Expect = 5.1e-29
Identity = 50/114 (43.86%), Postives = 77/114 (67.54%), Query Frame = 0


Query: 8   SETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSC 67
           S   ++ L +  +L+C  CF+PL  P+FQC NGH++C  CCIK   +CS C L IG + C
Sbjct: 71  SSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRC 130

Query: 68  RPIEKLLESVKKHCRNSIYGCK-TMIYWNKINDHESLCVYEPCSCPLSNCSFVG 121
           R +EK++++    C N+IYGCK +  Y N++  HE +CV+ PCSCP+ +C+++G
Sbjct: 131 RAMEKVIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIG 184

[Close]

BLAST of Moc08g35780 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 119.0 bits (297), Expect = 4.8e-27
Identity = 68/249 (27.31%), Postives = 112/249 (44.98%), Query Frame = 0


Query: 5   KENSETMNLILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGH 64
           K +++  + +L D ++LDC IC+E  + P+FQC NGH++CSSCC K +N+C +C   +GH
Sbjct: 33  KRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGH 92

Query: 65  MSCRPIEKLLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGSSGQ 124
             CR +E +LES+   C N+  GCK  + + K   HE  C++  C+CP  +C++  S   
Sbjct: 93  NRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKD 152

Query: 125 --------------------------------------EKVDETLYLLSYTSEPFGNAAT 184
                                                 E +   L+ +    EP+G   T
Sbjct: 153 LYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVT 212

Query: 185 VSRIG-RSMSMRKFHYEIKAKAEERVLCLQSIAKEIRAQVEVTSPSNGSLLIPKDFFESS 215
           VS I   S  + ++ Y +    +   +  QS   +   ++   +P    +LIP       
Sbjct: 213 VSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSFQTPQENFMLIPNSLLRG- 272

[Close]

BLAST of Moc08g35780 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 118.2 bits (295), Expect = 8.1e-27
Identity = 48/109 (44.04%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 13  LILTDPQLLDCYICFEPLSAPLFQCKNGHISCSSCCIKAHNQCSSCGLEIGHMSCRPIEK 72
           ++L D  +LDC IC E L++P+FQC NGH++C SCC K  N+C +C L +GH   R +E 
Sbjct: 28  IVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSRSRAMES 87

Query: 73  LLESVKKHCRNSIYGCKTMIYWNKINDHESLCVYEPCSCPLSNCSFVGS 122
           +LES+   C N  +GC    ++ K + HE  C++  CSCP S C + GS
Sbjct: 88  VLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGS 136

The following BLAST results are available for this feature:

BLAST of Moc08g35780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143329.1	9.4e-126	99.55	putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 [Momordica charantia...	[more]
XP_022143326.1	3.5e-120	85.27	putative E3 ubiquitin-protein ligase SINA-like 6 isoform X1 [Momordica charantia...	[more]
XP_022143327.1	1.1e-94	70.92	E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Momordica charantia]	[more]
XP_022143328.1	9.8e-91	69.51	E3 ubiquitin-protein ligase SINA-like 10 isoform X3 [Momordica charantia]	[more]
XP_022143325.1	3.7e-66	55.33	E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia]	[more]

BLAST of Moc08g35780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKD9	1.8e-31	46.22	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702...	[more]
Q84K34	2.9e-29	46.36	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9C9M0	7.2e-28	43.86	E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g6...	[more]
Q9FKD7	6.7e-26	27.31	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3...	[more]
Q9FKD5	1.1e-25	44.04	Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Moc08g35780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP04	4.5e-126	99.55	putative E3 ubiquitin-protein ligase SINA-like 6 isoform X4 OS=Momordica charant...	[more]
A0A6J1CPY7	1.7e-120	85.27	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A6J1CQH1	5.4e-95	70.92	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A6J1CQF8	4.7e-91	69.51	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A6J1CNI1	1.8e-66	55.33	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]

BLAST of Moc08g35780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37870.1	1.3e-32	46.22	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37930.1	2.1e-30	46.36	Protein with RING/U-box and TRAF-like domains	[more]
AT1G66650.1	5.1e-29	43.86	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	4.8e-27	27.31	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37910.1	8.1e-27	44.04	Protein with RING/U-box and TRAF-like domains	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 71..126 e-value: 2.0E-12 score: 49.1
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 124..217
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 5..123
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 76..203
IPR044286	E3 ubiquitin-protein ligase SINA-like, plant	PANTHER	PTHR46632	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 5..123 coord: 124..217
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 76..142 score: 9.859484

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc08g35780.1	Moc08g35780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding