Moc08g35750 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc08g35750
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionATP-dependent Clp protease proteolytic subunit
Locationchr8: 26359455 .. 26363019 (+)
RNA-Seq ExpressionMoc08g35750
SyntenyMoc08g35750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATGAGTTTCACATACACGCCTTCTCCTTCGCCTCAGAGGCTCATGCGCTTCGACCATGGAGTTCTAAGTTCTCAGTTTCCACTTTTCACTCCTCGGAAGCACTCCCATGGCGGCAATTCCAGTTTAAATGGTCTTAGGACCCCAAAATCGGCTCCTCTAGTGAAGGCCTCGCTCAGTTCTTCGTCGATGAGGCGGACCTTATCAAGTAATTGGGACGTTTTGGAGAATTTTTCTGCAACTTCCGCTCCATCGCTGGCTAGGTTCGAAGAGCTCGACACTACCAACATGCTTCTCCGCCAGCGAATCATCTTCTTGGGCTCTCAGGTAGTCTCCCTCTCTCTCTCTGTGAAACAAGATTCCGATTCCTGATTTCTGGACCAGTTGTGTTGAATATTTTTGCAGCTTTCTTAGGTTTAAATTGTGAGGTTTTGCTTTTTTCATTATGTTGAATTGGTTTTGTTTGGATGATTTGTGAATTGTTTAACTTGACTTGTAGACTACCTGTTACTGTTAGTGATCTATTGGTGCTACTGGCAGGTTGATGACATGACAGCAGATTTCATCATAAGCCAGCTACTGTTTCTTGATGCTGAAGACTCAACAAAAGACATCAAATTGTTTATCAACTCACCTGGTGGCTCTGTCACTGCTGGTATTTTTAGCATCATTTAATTTTCATCATGAAATCCTTGATTAATATTTTTAGAGTGATAAATTATAGTTCTGATACGAAGTTGTTGGAAGGGTGATCTCGTAAAAATTAAAATCTTTATTGTTCACACCTGCTTTCCACGGAATCCCTTCCCTTCCCTTCATTCTTCTAGGCTGGTTTTAGCCTTGGCCTCAAATAGGCTTCTTATCACTCTGTAAGCACGTATATGTAGAGTGTGAAGTATATTGCTAAATCCACATATATATTTTTTCACTTCAAAATGAGATCATGGCGCCTTATGTGTTGATCAAATGCAGATAATAGAATGGCAATGCTCTTTAGCTTTCCATCCTTTTAAATCGGAATGTCGGAATGTAGATCTCAATGCAAGATAAGCATATTTCAAAATTTTGGAATCATTGAAACATTTGAGATTCAGGTCCTAATCTGTTGTGCTTTGCTTTGACTTGATTATTCTCACTTTGGAAGTAGCTTGGGAAGTGTCATATCTGAGCCAGGATCATAATCCGTCTAGTTGTGAGCCAAGAGTGTCCGTTAGTGGTTGTGTTATTGTATTTTTGTTTTATGATAGTTTAATTCATTCATGTATTAGAGAGAACAGTGTGACATGCTTAGTTTTCAAAAAATATTCTTATTTCTCTTCCATGTTCAGTTAAACTAGTTATTACTTGTTACCACCATAGTTGTAACTTTTGCCATCAACCAAATGAAAAGTTTAGAATGTGGTGTGGCAATCATGGGGCAAGAGTTTGGATGATATTCATATTACCTGATTCAATTTCATTTCGCATGAAATAATATATTTTCTGATTAGGAGGGTTGCATAAACATACCCTTGCTGTGGGTTGGTTTCTTTCCTTCAACTGGCTTAAGAATTATATTACTGAAAATTGTGCAATGCCCATTCTGTCTAGTGAGTTCGACTTTCCTCCCCGTTGCCTCGTTATTTGTAAGCTGTAACTAGCATATGAACCCACTTGCCTCCCCAAGCATTTACCTCTTCTATCGTTTTCCCTCACTAATGCAGCCCTTATTATGTTCGTTTATCTTCTGTTCATCTCCTCCTTCTTCAATCATTTCAAGGCCTTCTCAAATTGAGCTTTGGAGTTCCACTTTTTCTTTCGGTCTACCTCTAATGTTGTGTGTTGTTTTTCATCCATATATGTTTCTCAACATTTTGTGGCTGCATGTAGGAATGGGAATATACGATGCAATGAAGTTGTGCAAGGCCGACGTTTCAACAGTTTGTCTAGGACTCGCTGCATCTATGGGAGCATTTCTTCTAGCTGCTGGTTCAAAGGGAAAGCGATTCTGCATGCCCAATGGGAGAGTGATGATCCATCAACCTCTCGGAACAGCTGGAGGGAAGGTGAGTACACTATTATTTTCTATGGGGCTGATGTTAAAAGTTAGATCATAAAGTTCATAGCTCGTTTTCCACCGTGATTGCATTTTTTTGTTTCACCTTCATTTTATTTACTTTTCTAGTCCCGGTTGAACTTGCTAAATATCTAAATTATTCTACGTTCATTCTATTAAGTGGTTGTGGAAACATGCAGATATATGTTTATGAGGATTTGATGTAGTTGTTGGAATTGTATGATGGCCATAGGGAGATCAAGATCAACAGCCATCTGAAAATACCAAAAATTTCTTCCAATTAAGTAATTCTTGAGTATTGCATAATCTAGTTGTTATAAACTTGAGTTATTTTGTGTGTGAACTTATTGATGGGAACATACCAGCCGTGCAGCATGTGTATTGTGTATACCCGAAGAGAGGACAGCAAAAAGAAGAAAGAGAGTTAGTTATGCTTTTTTAGAGTACGTGCACTAGAGTGAGCCCCATTTTTGAAATAGGTCGGACACGGCCATAGGTTGTGAGGGTGGGTTTATCTATGGGTTTAGGATCTCTTTTTACGCATCTGAGAATTCTGTTAGTATTTTGGCCTTGGGGAATATTTTTCCTTGAGGAGGGAGAGTTCTTGGCCTCTCAAAGAGCTAAGGTTACTGGCTGTTTGCGCCCTGATTTGGGATGATTAGTTGAATATATTGGAGATAGGCCATCTGAGGCCTATCACTTGTTTTTTTTTTTAGTCCAAAAGGTGGGGTGGGGGATCGAACTCACAACCTCTTGATTTTGGAGATATATGTCAATTACTGCTGAGCTATGCTCGCTTTGGGAAGGCCTATCACTGGTTTATAAAGACGCAATTGGCATTCTTTAGCTCGCCCACCTAGAGTGGGTATAAGGGCATAGAATCAATTATCAAATGCAGGAACTTTTCCCAAATTTTATAACTATTAATTTGCCCTGTTGATCTATATAATATGTAAATTTGAGCTTCCACTTATGGTAGTTCTCCCCTAAACACTTTGTAATTCATTGGTTACCCCGTTGTCCATGCCAGGCAACAGAGATGAGTATACGGATACGAGAAATGGCGTATCACAAGATCAAGCTGAACAAAATTTTTTCAAGAATCACAGGAAAGCCATTAGAGCAGGTTTTGCCTCTACATTGTTTCTTTAACTCTTCTCGGTTCTCAGTTTCTTTCTGAAACCCATGAAATTTATGAGTTGTCATTTGCAGATTGAATTGGACACGGACCGAGATAATTTCATGAATCCCTGGGAAGCTAAGGAATATGGTTTGATTGATGAAGTTATTGATGATGGGAAGCCAGGACTCATTGCTCCAATTGCAGATGCAGCTGCTCCGCCAAAAACCCGAGTCTGGGATCTATGGAAGGTGGAAGGGAGTAGGAAAGCCAAGAAGAATCTGCCTTCAGAAGACAAGATCTTGCAGAATGGATATCAAGGGGGTCAAGGAAACGACGAGGAGCGCGGTACCCAACAAGAAACAGAAGAACCGAGTCCTGTATGA

mRNA sequence

ATGGAGATGAGTTTCACATACACGCCTTCTCCTTCGCCTCAGAGGCTCATGCGCTTCGACCATGGAGTTCTAAGTTCTCAGTTTCCACTTTTCACTCCTCGGAAGCACTCCCATGGCGGCAATTCCAGTTTAAATGGTCTTAGGACCCCAAAATCGGCTCCTCTAGTGAAGGCCTCGCTCAGTTCTTCGTCGATGAGGCGGACCTTATCAAGTAATTGGGACGTTTTGGAGAATTTTTCTGCAACTTCCGCTCCATCGCTGGCTAGGTTCGAAGAGCTCGACACTACCAACATGCTTCTCCGCCAGCGAATCATCTTCTTGGGCTCTCAGGTTGATGACATGACAGCAGATTTCATCATAAGCCAGCTACTGTTTCTTGATGCTGAAGACTCAACAAAAGACATCAAATTGTTTATCAACTCACCTGGTGGCTCTGTCACTGCTGGAATGGGAATATACGATGCAATGAAGTTGTGCAAGGCCGACGTTTCAACAGTTTGTCTAGGACTCGCTGCATCTATGGGAGCATTTCTTCTAGCTGCTGGTTCAAAGGGAAAGCGATTCTGCATGCCCAATGGGAGAGTGATGATCCATCAACCTCTCGGAACAGCTGGAGGGAAGGCAACAGAGATGAGTATACGGATACGAGAAATGGCGTATCACAAGATCAAGCTGAACAAAATTTTTTCAAGAATCACAGGAAAGCCATTAGAGCAGATTGAATTGGACACGGACCGAGATAATTTCATGAATCCCTGGGAAGCTAAGGAATATGGTTTGATTGATGAAGTTATTGATGATGGGAAGCCAGGACTCATTGCTCCAATTGCAGATGCAGCTGCTCCGCCAAAAACCCGAGTCTGGGATCTATGGAAGGTGGAAGGGAGTAGGAAAGCCAAGAAGAATCTGCCTTCAGAAGACAAGATCTTGCAGAATGGATATCAAGGGGGTCAAGGAAACGACGAGGAGCGCGGTACCCAACAAGAAACAGAAGAACCGAGTCCTGTATGA

Coding sequence (CDS)

ATGGAGATGAGTTTCACATACACGCCTTCTCCTTCGCCTCAGAGGCTCATGCGCTTCGACCATGGAGTTCTAAGTTCTCAGTTTCCACTTTTCACTCCTCGGAAGCACTCCCATGGCGGCAATTCCAGTTTAAATGGTCTTAGGACCCCAAAATCGGCTCCTCTAGTGAAGGCCTCGCTCAGTTCTTCGTCGATGAGGCGGACCTTATCAAGTAATTGGGACGTTTTGGAGAATTTTTCTGCAACTTCCGCTCCATCGCTGGCTAGGTTCGAAGAGCTCGACACTACCAACATGCTTCTCCGCCAGCGAATCATCTTCTTGGGCTCTCAGGTTGATGACATGACAGCAGATTTCATCATAAGCCAGCTACTGTTTCTTGATGCTGAAGACTCAACAAAAGACATCAAATTGTTTATCAACTCACCTGGTGGCTCTGTCACTGCTGGAATGGGAATATACGATGCAATGAAGTTGTGCAAGGCCGACGTTTCAACAGTTTGTCTAGGACTCGCTGCATCTATGGGAGCATTTCTTCTAGCTGCTGGTTCAAAGGGAAAGCGATTCTGCATGCCCAATGGGAGAGTGATGATCCATCAACCTCTCGGAACAGCTGGAGGGAAGGCAACAGAGATGAGTATACGGATACGAGAAATGGCGTATCACAAGATCAAGCTGAACAAAATTTTTTCAAGAATCACAGGAAAGCCATTAGAGCAGATTGAATTGGACACGGACCGAGATAATTTCATGAATCCCTGGGAAGCTAAGGAATATGGTTTGATTGATGAAGTTATTGATGATGGGAAGCCAGGACTCATTGCTCCAATTGCAGATGCAGCTGCTCCGCCAAAAACCCGAGTCTGGGATCTATGGAAGGTGGAAGGGAGTAGGAAAGCCAAGAAGAATCTGCCTTCAGAAGACAAGATCTTGCAGAATGGATATCAAGGGGGTCAAGGAAACGACGAGGAGCGCGGTACCCAACAAGAAACAGAAGAACCGAGTCCTGTATGA

Protein sequence

MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV
Homology
BLAST of Moc08g35750 vs. NCBI nr
Match: XP_022143418.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Momordica charantia])

HSP 1 Score: 663.3 bits (1710), Expect = 1.1e-186
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60
           MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL
Sbjct: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60

Query: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII 120
           SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII
Sbjct: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII 120

Query: 121 SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA 180
           SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA
Sbjct: 121 SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA 180

Query: 181 AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI 240
           AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI
Sbjct: 181 AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI 240

Query: 241 ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK 300
           ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK
Sbjct: 241 ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK 300

Query: 301 KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 337
           KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV
Sbjct: 301 KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 336

BLAST of Moc08g35750 vs. NCBI nr
Match: XP_038902354.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 578.9 bits (1491), Expect = 2.8e-161
Identity = 290/336 (86.31%), Postives = 308/336 (91.67%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60
           ME+SFTY PS SP RLMR DHG+ SS FP+  P KHSHGGNS+ N  R   S P+V  S 
Sbjct: 1   MELSFTYAPSSSPHRLMRIDHGLPSSYFPISIPSKHSHGGNSTSNAFRIRNSVPVVNPSF 60

Query: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII 120
           +SSS+RRTLSS+WDVL+N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQVDDMTADFII
Sbjct: 61  NSSSLRRTLSSSWDVLDNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQVDDMTADFII 120

Query: 121 SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA 180
           SQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGAFLLA
Sbjct: 121 SQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGAFLLA 180

Query: 181 AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI 240
           +GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREM YHKIKLNKIFSRITGKPLEQI
Sbjct: 181 SGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMTYHKIKLNKIFSRITGKPLEQI 240

Query: 241 ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK 300
           ELDTDRDNFMNPWEAK+YGLIDEVIDDGKPGLIAP A+A  PPKTRVWDLWKVEGSRK K
Sbjct: 241 ELDTDRDNFMNPWEAKDYGLIDEVIDDGKPGLIAPTAEATPPPKTRVWDLWKVEGSRKTK 300

Query: 301 KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 337
           KNLPSE+KILQNGYQ GQG+DEERG+QQETEE SPV
Sbjct: 301 KNLPSENKILQNGYQEGQGSDEERGSQQETEETSPV 336

BLAST of Moc08g35750 vs. NCBI nr
Match: XP_008457103.1 (PREDICTED: ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucumis melo])

HSP 1 Score: 566.2 bits (1458), Expect = 1.8e-157
Identity = 291/339 (85.84%), Postives = 309/339 (91.15%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGV-LSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK- 60
           ME+SFT+ PSPSP  L RFDHG+  SS  P+ TP  HSHG N + N  R    S P++  
Sbjct: 1   MELSFTHAPSPSPYGLKRFDHGLHHSSHVPISTPSNHSHGRNFTSNNFRIRNCSVPVLNH 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTAD 120
            SLSSSS+RRTLSSNWDVL N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQVDD+TAD
Sbjct: 61  PSLSSSSLRRTLSSNWDVLSNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQVDDVTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL
Sbjct: 181 LLASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWKVEGSR
Sbjct: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRVWDLWKVEGSR 300

Query: 301 KAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 337
           KAKKNLPSE KIL+NGYQGG+G+DEERG+QQETEEPSPV
Sbjct: 301 KAKKNLPSESKILKNGYQGGEGSDEERGSQQETEEPSPV 339

BLAST of Moc08g35750 vs. NCBI nr
Match: XP_022948597.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 558.9 bits (1439), Expect = 2.9e-155
Identity = 290/338 (85.80%), Postives = 305/338 (90.24%), Query Frame = 0

Query: 1   MEMSFTYTPSPS-PQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPL--VK 60
           ME+SFTY PSPS   RLM F+HGVL+S FP+ TP KHSHG NSS N  R  KS P+  VK
Sbjct: 1   MELSFTYAPSPSLHSRLMCFNHGVLASPFPVSTPFKHSHGANSSSNAFRIRKSVPVAAVK 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTAD 120
           ASLSSS +RRTLSSNWDVL N+SA SAP+L RFEELDTTNMLLRQRIIFLGSQVDDMTAD
Sbjct: 61  ASLSSSPLRRTLSSNWDVLGNYSAASAPALPRFEELDTTNMLLRQRIIFLGSQVDDMTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKL INSPGGSVTAGMGIYDAMKLCKADVSTVC+GLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLLINSPGGSVTAGMGIYDAMKLCKADVSTVCMGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLAAGSKGKRFCMPNGRVMIHQPLGTAGG+AT+MSIR+REMAYHKIKLNKI SRITGKPL
Sbjct: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGRATDMSIRLREMAYHKIKLNKILSRITGKPL 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWK+EGSR
Sbjct: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEANPPPKTRVWDLWKIEGSR 300

Query: 301 KAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSP 336
           KAKKNLPSE KILQNGY   QG+DEER  Q ETEEPSP
Sbjct: 301 KAKKNLPSETKILQNGY---QGSDEERSPQPETEEPSP 335

BLAST of Moc08g35750 vs. NCBI nr
Match: XP_011651427.1 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucumis sativus] >KGN57884.1 hypothetical protein Csa_010181 [Cucumis sativus])

HSP 1 Score: 558.9 bits (1439), Expect = 2.9e-155
Identity = 288/339 (84.96%), Postives = 305/339 (89.97%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK-A 60
           ME+SFT+ PSPSP  L R DHG  SS FP+ TP KHSHGGN + N  R    S P+V   
Sbjct: 1   MELSFTHAPSPSPYGLKRSDHGFQSSHFPISTPSKHSHGGNFTSNAFRIRNCSVPVVNHP 60

Query: 61  SLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADF 120
           S SSSS+RRTLSSNWDV  N+S +SA SL RFEELDTTNMLLRQRIIFLGSQVDDMTADF
Sbjct: 61  SHSSSSLRRTLSSNWDVFSNYSVSSARSLPRFEELDTTNMLLRQRIIFLGSQVDDMTADF 120

Query: 121 IISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFL 180
           IISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFL
Sbjct: 121 IISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFL 180

Query: 181 LAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLE 240
           LA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP+E
Sbjct: 181 LASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPIE 240

Query: 241 QIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRK 300
           QIELDT+RDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTR+WDLWKVEGSRK
Sbjct: 241 QIELDTERDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRIWDLWKVEGSRK 300

Query: 301 AKKNLPSEDKILQNGY-QGGQGNDEERGTQQETEEPSPV 337
           AKKNLPSE KIL+NGY +GGQG+D+ERG QQETEEPSPV
Sbjct: 301 AKKNLPSESKILKNGYEEGGQGSDKERGGQQETEEPSPV 339

BLAST of Moc08g35750 vs. ExPASy Swiss-Prot
Match: Q9SXJ6 (ATP-dependent Clp protease proteolytic subunit 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPP3 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.8e-113
Identity = 218/305 (71.48%), Postives = 242/305 (79.34%), Query Frame = 0

Query: 17  MRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAP--LVKASLS-SSSMRRTLSSNW 76
           +R      S+   L  P K+    N  +   R PK++    V++S+S S   R+TLSSNW
Sbjct: 5   LRLASSSTSNPICLLNPGKNL---NFPIRNHRIPKTSKPFCVRSSMSLSKPPRQTLSSNW 64

Query: 77  DVLENFS----ATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAE 136
           DV  +FS    A S   L  FEELDTTNMLLRQRI+FLGSQVDDMTAD +ISQLL LDAE
Sbjct: 65  DV-SSFSIDSVAQSPSRLPSFEELDTTNMLLRQRIVFLGSQVDDMTADLVISQLLLLDAE 124

Query: 137 DSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFC 196
           DS +DI LFINSPGGS+TAGMGIYDAMK CKADVSTVCLGLAASMGAFLLA+GSKGKR+C
Sbjct: 125 DSERDITLFINSPGGSITAGMGIYDAMKQCKADVSTVCLGLAASMGAFLLASGSKGKRYC 184

Query: 197 MPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNF 256
           MPN +VMIHQPLGTAGGKATEMSIRIREM YHKIKLNKIFSRITGKP  +IE DTDRDNF
Sbjct: 185 MPNSKVMIHQPLGTAGGKATEMSIRIREMMYHKIKLNKIFSRITGKPESEIESDTDRDNF 244

Query: 257 MNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDKI 315
           +NPWEAKEYGLID VIDDGKPGLIAPI D   PPKT+VWDLWKVEG++K   NLPSE  +
Sbjct: 245 LNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWDLWKVEGTKKDNTNLPSERSM 304

BLAST of Moc08g35750 vs. ExPASy Swiss-Prot
Match: Q8YXH5 (ATP-dependent Clp protease proteolytic subunit 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 109/173 (63.01%), Postives = 138/173 (79.77%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIY 153
           D  + LLR+RI+FLG QVD   A+ I++QLLFLDAED  KDI L+INSPGGSVTAGMGI+
Sbjct: 18  DIYSRLLRERIVFLGQQVDSNLANLIVAQLLFLDAEDPEKDIYLYINSPGGSVTAGMGIF 77

Query: 154 DAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSI 213
           D MK  + DV T+C GLAASMGAFLL+AG+KGKR  +P+ R+MIHQPLG A G+AT++ I
Sbjct: 78  DTMKHIRPDVCTICTGLAASMGAFLLSAGAKGKRMSLPHSRIMIHQPLGGAQGQATDIEI 137

Query: 214 RIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVID 267
           + RE+ YHK +LN   +  TG+P+E+I  DT+RD FM+P EAK+YGLID+VID
Sbjct: 138 QAREILYHKRRLNDYLAEHTGQPIERIAEDTERDFFMSPDEAKDYGLIDQVID 190

BLAST of Moc08g35750 vs. ExPASy Swiss-Prot
Match: Q3MFR5 (ATP-dependent Clp protease proteolytic subunit 1 OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=clpP1 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.3e-56
Identity = 109/173 (63.01%), Postives = 138/173 (79.77%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIY 153
           D  + LLR+RIIFLG QVD   A+ I++QLLFLDAED  KDI L+INSPGGSVTAGMGI+
Sbjct: 18  DIYSRLLRERIIFLGQQVDSNLANLIVAQLLFLDAEDPEKDIYLYINSPGGSVTAGMGIF 77

Query: 154 DAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSI 213
           D MK  + DV T+C GLAASMGAFLL+AG+KGKR  +P+ R+MIHQPLG A G+AT++ I
Sbjct: 78  DTMKHIRPDVCTICTGLAASMGAFLLSAGTKGKRMSLPHSRIMIHQPLGGAQGQATDIEI 137

Query: 214 RIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVID 267
           + RE+ YHK +LN   +  TG+P+E+I  DT+RD FM+P EA++YGLID+VID
Sbjct: 138 QAREILYHKRRLNDYLAEHTGQPIERIAEDTERDFFMSPDEARDYGLIDQVID 190

BLAST of Moc08g35750 vs. ExPASy Swiss-Prot
Match: O34125 (ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=clpP2 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.3e-56
Identity = 106/172 (61.63%), Postives = 138/172 (80.23%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIY 153
           D  + LLR+RI+FLG+ VDD  AD I++QLLFL+AED  KDI+L+INSPGGSVTAGM IY
Sbjct: 53  DIYSRLLRERIVFLGTGVDDAVADSIVAQLLFLEAEDPEKDIQLYINSPGGSVTAGMAIY 112

Query: 154 DAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSI 213
           D M+    DV+T+C GLAASMGAFLL+ G++GKR  +P+ R+MIHQPLG A G+A ++ I
Sbjct: 113 DTMQQVAPDVATICFGLAASMGAFLLSGGAQGKRMALPSARIMIHQPLGGAQGQAVDIEI 172

Query: 214 RIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVI 266
           + RE+ YHK  LN + ++ TG+PLE+IE+DTDRD FM+P EAK YGLID+V+
Sbjct: 173 QAREILYHKSTLNDLLAQHTGQPLEKIEVDTDRDFFMSPEEAKAYGLIDQVL 224

BLAST of Moc08g35750 vs. ExPASy Swiss-Prot
Match: Q5N1P6 (ATP-dependent Clp protease proteolytic subunit 3 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=clpP3 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.3e-56
Identity = 106/172 (61.63%), Postives = 138/172 (80.23%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIY 153
           D  + LLR+RI+FLG+ VDD  AD I++QLLFL+AED  KDI+L+INSPGGSVTAGM IY
Sbjct: 53  DIYSRLLRERIVFLGTGVDDAVADSIVAQLLFLEAEDPEKDIQLYINSPGGSVTAGMAIY 112

Query: 154 DAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSI 213
           D M+    DV+T+C GLAASMGAFLL+ G++GKR  +P+ R+MIHQPLG A G+A ++ I
Sbjct: 113 DTMQQVAPDVATICFGLAASMGAFLLSGGAQGKRMALPSARIMIHQPLGGAQGQAVDIEI 172

Query: 214 RIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVI 266
           + RE+ YHK  LN + ++ TG+PLE+IE+DTDRD FM+P EAK YGLID+V+
Sbjct: 173 QAREILYHKSTLNDLLAQHTGQPLEKIEVDTDRDFFMSPEEAKAYGLIDQVL 224

BLAST of Moc08g35750 vs. ExPASy TrEMBL
Match: A0A6J1CQN6 (ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN=LOC111013299 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.4e-187
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60
           MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL
Sbjct: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASL 60

Query: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII 120
           SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII
Sbjct: 61  SSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFII 120

Query: 121 SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA 180
           SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA
Sbjct: 121 SQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLA 180

Query: 181 AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI 240
           AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI
Sbjct: 181 AGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQI 240

Query: 241 ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK 300
           ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK
Sbjct: 241 ELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAK 300

Query: 301 KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 337
           KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV
Sbjct: 301 KNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 336

BLAST of Moc08g35750 vs. ExPASy TrEMBL
Match: A0A1S3C615 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103496858 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 8.9e-158
Identity = 291/339 (85.84%), Postives = 309/339 (91.15%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGV-LSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK- 60
           ME+SFT+ PSPSP  L RFDHG+  SS  P+ TP  HSHG N + N  R    S P++  
Sbjct: 1   MELSFTHAPSPSPYGLKRFDHGLHHSSHVPISTPSNHSHGRNFTSNNFRIRNCSVPVLNH 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTAD 120
            SLSSSS+RRTLSSNWDVL N+SA+SAPSL RFEELDTTNMLLRQRIIFLGSQVDD+TAD
Sbjct: 61  PSLSSSSLRRTLSSNWDVLSNYSASSAPSLPRFEELDTTNMLLRQRIIFLGSQVDDVTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVST+CLGLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTICLGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL
Sbjct: 181 LLASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWKVEGSR
Sbjct: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRVWDLWKVEGSR 300

Query: 301 KAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSPV 337
           KAKKNLPSE KIL+NGYQGG+G+DEERG+QQETEEPSPV
Sbjct: 301 KAKKNLPSESKILKNGYQGGEGSDEERGSQQETEEPSPV 339

BLAST of Moc08g35750 vs. ExPASy TrEMBL
Match: A0A0A0LA84 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G363170 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 288/339 (84.96%), Postives = 305/339 (89.97%), Query Frame = 0

Query: 1   MEMSFTYTPSPSPQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPK-SAPLVK-A 60
           ME+SFT+ PSPSP  L R DHG  SS FP+ TP KHSHGGN + N  R    S P+V   
Sbjct: 1   MELSFTHAPSPSPYGLKRSDHGFQSSHFPISTPSKHSHGGNFTSNAFRIRNCSVPVVNHP 60

Query: 61  SLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADF 120
           S SSSS+RRTLSSNWDV  N+S +SA SL RFEELDTTNMLLRQRIIFLGSQVDDMTADF
Sbjct: 61  SHSSSSLRRTLSSNWDVFSNYSVSSARSLPRFEELDTTNMLLRQRIIFLGSQVDDMTADF 120

Query: 121 IISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFL 180
           IISQLLFLDAED TKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFL
Sbjct: 121 IISQLLFLDAEDPTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFL 180

Query: 181 LAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLE 240
           LA+GSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKP+E
Sbjct: 181 LASGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPIE 240

Query: 241 QIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRK 300
           QIELDT+RDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTR+WDLWKVEGSRK
Sbjct: 241 QIELDTERDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEATPPPKTRIWDLWKVEGSRK 300

Query: 301 AKKNLPSEDKILQNGY-QGGQGNDEERGTQQETEEPSPV 337
           AKKNLPSE KIL+NGY +GGQG+D+ERG QQETEEPSPV
Sbjct: 301 AKKNLPSESKILKNGYEEGGQGSDKERGGQQETEEPSPV 339

BLAST of Moc08g35750 vs. ExPASy TrEMBL
Match: A0A6J1G9P1 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452225 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.4e-155
Identity = 290/338 (85.80%), Postives = 305/338 (90.24%), Query Frame = 0

Query: 1   MEMSFTYTPSPS-PQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPL--VK 60
           ME+SFTY PSPS   RLM F+HGVL+S FP+ TP KHSHG NSS N  R  KS P+  VK
Sbjct: 1   MELSFTYAPSPSLHSRLMCFNHGVLASPFPVSTPFKHSHGANSSSNAFRIRKSVPVAAVK 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTAD 120
           ASLSSS +RRTLSSNWDVL N+SA SAP+L RFEELDTTNMLLRQRIIFLGSQVDDMTAD
Sbjct: 61  ASLSSSPLRRTLSSNWDVLGNYSAASAPALPRFEELDTTNMLLRQRIIFLGSQVDDMTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKL INSPGGSVTAGMGIYDAMKLCKADVSTVC+GLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLLINSPGGSVTAGMGIYDAMKLCKADVSTVCMGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLAAGSKGKRFCMPNGRVMIHQPLGTAGG+AT+MSIR+REMAYHKIKLNKI SRITGKPL
Sbjct: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGRATDMSIRLREMAYHKIKLNKILSRITGKPL 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWK+EGSR
Sbjct: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEANPPPKTRVWDLWKIEGSR 300

Query: 301 KAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSP 336
           KAKKNLPSE KILQNGY   QG+DEER  Q ETEEPSP
Sbjct: 301 KAKKNLPSETKILQNGY---QGSDEERSPQPETEEPSP 335

BLAST of Moc08g35750 vs. ExPASy TrEMBL
Match: A0A6J1KEX5 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111492640 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.7e-153
Identity = 286/338 (84.62%), Postives = 303/338 (89.64%), Query Frame = 0

Query: 1   MEMSFTYTPSPS-PQRLMRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAPL--VK 60
           ME+SFTY  SPS   RLM ++HGVL+S FP+ TP KHSHG NSS N  R  KS P+  VK
Sbjct: 1   MELSFTYAASPSLHSRLMCYNHGVLASPFPVSTPFKHSHGANSSSNAFRIRKSVPVAAVK 60

Query: 61  ASLSSSSMRRTLSSNWDVLENFSATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTAD 120
           ASLSSS +RRTLSSNWDVL N+SA S P+L RFEELDTTNMLLRQRIIFLGSQVDDMTAD
Sbjct: 61  ASLSSSPLRRTLSSNWDVLGNYSAASTPALPRFEELDTTNMLLRQRIIFLGSQVDDMTAD 120

Query: 121 FIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAF 180
           FIISQLLFLDAED TKDIKL INSPGGSVTAGMGIYDAMKLCKADVSTVC+GLAASMGAF
Sbjct: 121 FIISQLLFLDAEDPTKDIKLLINSPGGSVTAGMGIYDAMKLCKADVSTVCMGLAASMGAF 180

Query: 181 LLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPL 240
           LLA+GSKGKRFCMPNGRVMIHQPLGTAGG+AT+MSIR+REMAYHKIKLNKI SRITGKPL
Sbjct: 181 LLASGSKGKRFCMPNGRVMIHQPLGTAGGRATDMSIRLREMAYHKIKLNKILSRITGKPL 240

Query: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSR 300
           EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIA+A  PPKTRVWDLWK+EGSR
Sbjct: 241 EQIELDTDRDNFMNPWEAKEYGLIDEVIDDGKPGLIAPIAEANPPPKTRVWDLWKIEGSR 300

Query: 301 KAKKNLPSEDKILQNGYQGGQGNDEERGTQQETEEPSP 336
           KAKKNLPSE KILQNGY   QG+DEER  Q ETEEPSP
Sbjct: 301 KAKKNLPSETKILQNGY---QGSDEERSPQPETEEPSP 335

BLAST of Moc08g35750 vs. TAIR 10
Match: AT1G66670.1 (CLP protease proteolytic subunit 3 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.7e-114
Identity = 218/305 (71.48%), Postives = 242/305 (79.34%), Query Frame = 0

Query: 17  MRFDHGVLSSQFPLFTPRKHSHGGNSSLNGLRTPKSAP--LVKASLS-SSSMRRTLSSNW 76
           +R      S+   L  P K+    N  +   R PK++    V++S+S S   R+TLSSNW
Sbjct: 5   LRLASSSTSNPICLLNPGKNL---NFPIRNHRIPKTSKPFCVRSSMSLSKPPRQTLSSNW 64

Query: 77  DVLENFS----ATSAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAE 136
           DV  +FS    A S   L  FEELDTTNMLLRQRI+FLGSQVDDMTAD +ISQLL LDAE
Sbjct: 65  DV-SSFSIDSVAQSPSRLPSFEELDTTNMLLRQRIVFLGSQVDDMTADLVISQLLLLDAE 124

Query: 137 DSTKDIKLFINSPGGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFC 196
           DS +DI LFINSPGGS+TAGMGIYDAMK CKADVSTVCLGLAASMGAFLLA+GSKGKR+C
Sbjct: 125 DSERDITLFINSPGGSITAGMGIYDAMKQCKADVSTVCLGLAASMGAFLLASGSKGKRYC 184

Query: 197 MPNGRVMIHQPLGTAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNF 256
           MPN +VMIHQPLGTAGGKATEMSIRIREM YHKIKLNKIFSRITGKP  +IE DTDRDNF
Sbjct: 185 MPNSKVMIHQPLGTAGGKATEMSIRIREMMYHKIKLNKIFSRITGKPESEIESDTDRDNF 244

Query: 257 MNPWEAKEYGLIDEVIDDGKPGLIAPIADAAAPPKTRVWDLWKVEGSRKAKKNLPSEDKI 315
           +NPWEAKEYGLID VIDDGKPGLIAPI D   PPKT+VWDLWKVEG++K   NLPSE  +
Sbjct: 245 LNPWEAKEYGLIDAVIDDGKPGLIAPIGDGTPPPKTKVWDLWKVEGTKKDNTNLPSERSM 304

BLAST of Moc08g35750 vs. TAIR 10
Match: AT5G45390.1 (CLP protease P4 )

HSP 1 Score: 201.8 bits (512), Expect = 8.5e-52
Identity = 115/260 (44.23%), Postives = 158/260 (60.77%), Query Frame = 0

Query: 25  SSQFPLFTPRKHSHGGNSSLNGLRTPKSAPLVKASLSSSSMRRTLSS--NWDVLENFSAT 84
           SS  P     + +   ++S +    P +  L    L S  +R T  S   +       + 
Sbjct: 8   SSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPTKLISPPLRTTSPSPLRFANASIEMSQ 67

Query: 85  SAPSLARFEELDTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSP 144
           +  S  R  E D   +LLR+RI+FLGS +DD  AD I+SQLL LDA+D  KDIKLFINSP
Sbjct: 68  TQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLLDAKDPKKDIKLFINSP 127

Query: 145 GGSVTAGMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLG 204
           GGS++A M IYD ++L +ADVST+ LG+AAS  + +L AG+KGKRF MPN R+MIHQPLG
Sbjct: 128 GGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGKRFAMPNTRIMIHQPLG 187

Query: 205 TAGGKATEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLID 264
            A G+A ++ I+ +E+ ++K  +  I +  T +  EQ+  D DRD +M+P EA EYGLID
Sbjct: 188 GASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDRDRYMSPIEAVEYGLID 247

Query: 265 EVIDDGKPGLIAPIADAAAP 283
            VID      + P+ D   P
Sbjct: 248 GVIDGDSIIPLEPVPDRVKP 267

BLAST of Moc08g35750 vs. TAIR 10
Match: AT1G02560.1 (nuclear encoded CLP protease 5 )

HSP 1 Score: 199.5 bits (506), Expect = 4.2e-51
Identity = 103/177 (58.19%), Postives = 131/177 (74.01%), Query Frame = 0

Query: 89  RFEELDTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTA 148
           RF+ +   + L + RII  G  VDD  A+ I++QLL+LDA D TKDI +++NSPGGSVTA
Sbjct: 111 RFQSI--ISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVTA 170

Query: 149 GMGIYDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKA 208
           GM I+D M+  + DVSTVC+GLAASMGAFLL+AG+KGKR+ +PN R+MIHQPLG A G  
Sbjct: 171 GMAIFDTMRHIRPDVSTVCVGLAASMGAFLLSAGTKGKRYSLPNSRIMIHQPLGGAQGGQ 230

Query: 209 TEMSIRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVI 266
           T++ I+  EM +HK  LN   +  TG+ LE+I  DTDRD FM+  EAKEYGLID VI
Sbjct: 231 TDIDIQANEMLHHKANLNGYLAYHTGQSLEKINQDTDRDFFMSAKEAKEYGLIDGVI 285

BLAST of Moc08g35750 vs. TAIR 10
Match: AT5G23140.1 (nuclear-encoded CLP protease P7 )

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-41
Identity = 79/174 (45.40%), Postives = 126/174 (72.41%), Query Frame = 0

Query: 94  DTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGIY 153
           D  + LL++RII +   ++D T+  +++QLL+L++E+ +K I +++NSPGG VTAG+ IY
Sbjct: 48  DIFSRLLKERIICINGPINDDTSHVVVAQLLYLESENPSKPIHMYLNSPGGHVTAGLAIY 107

Query: 154 DAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMSI 213
           D M+  ++ +ST+CLG AASM + LLAAG+KG+R  +PN  VMIHQP G   G+A +++I
Sbjct: 108 DTMQYIRSPISTICLGQAASMASLLLAAGAKGQRRSLPNATVMIHQPSGGYSGQAKDITI 167

Query: 214 RIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVIDD 268
             +++      LN+++ + TG+PL+ +  + DRD+FM P EAK +G+IDEVID+
Sbjct: 168 HTKQIVRVWDALNELYVKHTGQPLDVVANNMDRDHFMTPEEAKAFGIIDEVIDE 221

BLAST of Moc08g35750 vs. TAIR 10
Match: AT1G11750.1 (CLP protease proteolytic subunit 6 )

HSP 1 Score: 149.1 bits (375), Expect = 6.6e-36
Identity = 74/174 (42.53%), Postives = 112/174 (64.37%), Query Frame = 0

Query: 93  LDTTNMLLRQRIIFLGSQVDDMTADFIISQLLFLDAEDSTKDIKLFINSPGGSVTAGMGI 152
           LD +++L R RIIF+G  ++   A  +ISQL+ L + D   DI +++N PGGS  + + I
Sbjct: 95  LDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLNCPGGSTYSVLAI 154

Query: 153 YDAMKLCKADVSTVCLGLAASMGAFLLAAGSKGKRFCMPNGRVMIHQPLGTAGGKATEMS 212
           YD M   K  V TV  G+AAS GA LLA G KG R+ MPN RVMIHQP    GG   ++ 
Sbjct: 155 YDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQPQTGCGGHVEDVR 214

Query: 213 IRIREMAYHKIKLNKIFSRITGKPLEQIELDTDRDNFMNPWEAKEYGLIDEVID 267
            ++ E    + K++++++  TG+PLE+++  T+RD F++  EA E+GLID +++
Sbjct: 215 RQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGLIDGLLE 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143418.11.1e-186100.00ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Momordica chara... [more]
XP_038902354.12.8e-16186.31ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Benincasa hispi... [more]
XP_008457103.11.8e-15785.84PREDICTED: ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucu... [more]
XP_022948597.12.9e-15585.80ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucurbita mosch... [more]
XP_011651427.12.9e-15584.96ATP-dependent Clp protease proteolytic subunit 3, chloroplastic [Cucumis sativus... [more]
Match NameE-valueIdentityDescription
Q9SXJ63.8e-11371.48ATP-dependent Clp protease proteolytic subunit 3, chloroplastic OS=Arabidopsis t... [more]
Q8YXH51.9e-5663.01ATP-dependent Clp protease proteolytic subunit 1 OS=Nostoc sp. (strain PCC 7120 ... [more]
Q3MFR53.3e-5663.01ATP-dependent Clp protease proteolytic subunit 1 OS=Trichormus variabilis (strai... [more]
O341257.3e-5661.63ATP-dependent Clp protease proteolytic subunit 2 OS=Synechococcus elongatus (str... [more]
Q5N1P67.3e-5661.63ATP-dependent Clp protease proteolytic subunit 3 OS=Synechococcus sp. (strain AT... [more]
Match NameE-valueIdentityDescription
A0A6J1CQN65.4e-187100.00ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN... [more]
A0A1S3C6158.9e-15885.84ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LA841.4e-15584.96ATP-dependent Clp protease proteolytic subunit OS=Cucumis sativus OX=3659 GN=Csa... [more]
A0A6J1G9P11.4e-15585.80ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KEX51.7e-15384.62ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G66670.12.7e-11471.48CLP protease proteolytic subunit 3 [more]
AT5G45390.18.5e-5244.23CLP protease P4 [more]
AT1G02560.14.2e-5158.19nuclear encoded CLP protease 5 [more]
AT5G23140.12.3e-4145.40nuclear-encoded CLP protease P7 [more]
AT1G11750.16.6e-3642.53CLP protease proteolytic subunit 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001907ATP-dependent Clp protease proteolytic subunitPRINTSPR00127CLPPROTEASEPcoord: 243..262
score: 58.0
coord: 165..182
score: 68.61
coord: 94..109
score: 46.25
coord: 186..205
score: 52.0
coord: 134..154
score: 64.64
IPR001907ATP-dependent Clp protease proteolytic subunitHAMAPMF_00444ClpPcoord: 75..268
score: 36.987617
IPR001907ATP-dependent Clp protease proteolytic subunitCDDcd07017S14_ClpP_2coord: 94..264
e-value: 1.27327E-102
score: 297.046
NoneNo IPR availableGENE3D3.90.226.10coord: 79..269
e-value: 1.8E-71
score: 241.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 308..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..336
NoneNo IPR availablePANTHERPTHR10381:SF50ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 3, CHLOROPLASTICcoord: 66..312
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPFAMPF00574CLP_proteasecoord: 93..266
e-value: 1.0E-73
score: 247.1
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPANTHERPTHR10381ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNITcoord: 66..312
IPR033135ClpP, histidine active sitePROSITEPS00382CLP_PROTEASE_HIScoord: 187..200
IPR018215ClpP, Ser active sitePROSITEPS00381CLP_PROTEASE_SERcoord: 165..176
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 93..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc08g35750.1Moc08g35750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
biological_process GO:0006508 proteolysis
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0009368 endopeptidase Clp complex
cellular_component GO:0009526 plastid envelope
molecular_function GO:0051117 ATPase binding
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0004252 serine-type endopeptidase activity